MD03G1006300.v1.1

acyl-activating enzyme 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005780
Physical Location & Seq
Forward (+)
520337 .. 525242
4906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1006300.v1.1.491

Sequence Viewer

Length: 2187 bp
ATGGTGAAGAACATAAGTGAGGTGGGAGTGGAGAACTTTGTTCAGGCAGGGCTAGACGTTGAAGAGGCCAAGGATTTCGATAGAGTCCTCAAAAATACAATCTCCCGCACGAACACAACGGACCCAAAAGAGATATGGCGGCAGATAGTGGCTGAGAGATTGCTGAAACCATCACACCCTCATGAGCTGCACCAGTTGATCTACTACTCAGTCTATGCCAATTGGGATGCCTCCACCAAAGGCCCTCCTCTCTACTGGTTCCCTTCTCTACACCAGTCGCGACAAACAAACTTGGGGCGATTGATGGAAAAGCACGGTTCAGAGCTTTTAGGAACATCATATAAGGATCCCATAACAAGTTATACACTCTTCCAGGAATTCTCTGTTGGGAATCCCGAGGCTTACTGGTCACTTGTTCTAAAAGAGCTCTCGGTTTCATTTCGAAAAGTCCCAAAGTGTATTTTGGATAGGACTGACAAATCAAAACGCAGTGGATCCTGGCTTCCACATTCAGTTTTCAATATTGCTGAATGTTGTTTGCTACCCACCAGCCACCCAAGAAAAGAGGATGATAGTTTGGCTGTTGTATGGAGGGATGAAGGTTGCGACGATGATTCTTTTGTTAATCATATGACATTAAAGGAACTTCGGGAACAAGTAATGTTGGTGGCTAATGCACTGGATGCAACCTTTTCAAAGGGTGATGCAATTGCTATCGACATGCCAATGAATGTCAATGCGGTTATCATATATTTAGCAATTGTACTAGCAGGATTCGTTGTTGTATCAATAGCAGACAGTTTTGCTACAAATGAAATTGCAACTCGTCTACGCGTGTCTAAAGCAAAGGGTGTCTTTACCCAGGATTTCATACTAAGGGGAGGCCGGAAGTTTCCTCTCTACAGTCGAGTTGCAGAGGCCGCTCCACATTTAGCTATTGTGCTTCCAGCAATTGGGAGCAATGTAGGCATTCAGTTAAGAGAGCAGGATCTATCCTGGCATGATTTTCTTTCTCGTGTCGATCACCTTCCAAGAAAGAACTACTACTCTCCAGTCTATCAGCCAGTCGACTCCGTGACTAATATTCTCTTTTCATCTGGAACCACAGGAGATCCGAAAGCTATTCCATGGACACAACTTTCACCAATTAGATGTGCTGCTGATTCATGGTCTCACAATGATAATCAAGTTGGGGATGTCTTCTGCTGGCCAACAAATTTGGGATGGGTTATGGGTCCAATCTTACTCTACTCGTGCTTTCTAACTGGTTCAACTCTTGCTCTGTATCATGGGTCTCCTCTCAGTCGTGGCTTTGGAAAATTTGTTCAGGATGCCAGAGTAACTATATTGGGCACAGTTCCAAGCTTAGTAAAGGCTTGGAAGAGTACACAGTGTATGAAAGGCCTAGACTGGACAAAGATAAGATCATTTTGTTCCACTGGTGAAGCGTCTAGCGTTGATGATGACCTTTGGCTCGCTTCCCGTGCTTACTATGGACCCATCATTGAATGTTGTGGGGGCACAGAGCTTGCTTCGTCCTACATCATGGGAAGTCAGCTACAGCCACAAGCTTTTGGAGCATTTAGCACAAAGTCGATGACAACAGGCCTTGTCATCTTTGATGAACATGGAATTCCTTATCCAGAGGAGCAAGCTTGTGTTGGGGAAGTGGGTTTGTTCCCGCAATTAATGGGAGCGACTGACAGGCTGCTAAATGCTGATCATGAAAAAGTTTACTTCAAGGGAATGCCGATGTATAATGGAACGCAACTTAGGAGACACGGAGACATACTGAAAAGAACTGTTGGAGGCTATTTGATTGTTCAAGGCAGGTCCGATGATACCATGAACCTCGGTGGCATCAAGACAAGTTCTGTTGAAATTGAGCGCGTCTGTGACCGGGCTGATGAAAGCATCTTGGAGACAGCTGCAGTCAGTGTTGCACCCAAGAGTGGTGGTCCTGAACAGTTGATTATATTTGTCGTATTAAATAAGGGATATAACTCCGAACCAGACGAGCTGAAGAAGAAATTCTCAAAAGCCATTCAAAGCAACCTTAACCCTTTGTTTAAGGTGAGCTTGGTCAAGATTGTACCAGAATTTCCTCGAACAGCGTCCAACAAGTTACTAAGAAGAGTTTTGAGGGACCAAGTTAAGCATGAGCTCTCTGTTCGGAGCAGAATGTAG

Protein Analysis

729

Amino Acids

81.12

Weight (kDa)

7.24

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 191 - 556 2.2e-36 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1821
AccB7I CCANNNNNTGG 1 cut(s) 953
AccBSI CCGCTC 1 cut(s) 923
AccI GTMKAC 2 cut(s) 829, 1068
AccII CGCG 3 cut(s) 280, 834, 1890
AciI CCGC 5 cut(s) 106, 139, 740, 921, 1682
AclWI GGATC 6 cut(s) 341, 354, 489, 502, 996, 1106
AcoI YGGCCR 1 cut(s) 1208
AcsI RAATTY 6 cut(s) 377, 1216, 1319, 1632, 2030, 2099
AcuI CTGAAG 1 cut(s) 2042
AfaI GTAC 3 cut(s) 765, 1387, 2094
AfiI CCNNNNNNNGG 2 cut(s) 953, 1952
AflIII ACRYGT 1 cut(s) 832
AgsI TTSAA 9 cut(s) 62, 520, 696, 1272, 1508, 1741, 1826, 1880, 2048
AjnI CCWGG 4 cut(s) 372, 497, 861, 995
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AloI GAACNNNNNNTCC 2 cut(s) 1308, 1340
Alw21I GWGCWC 2 cut(s) 429, 2166
Alw26I GTCTC 5 cut(s) 1176, 1299, 1771, 1779, 1916
AlwI GGATC 6 cut(s) 341, 354, 489, 502, 996, 1106
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 7 cut(s) 66, 241, 883, 918, 1208, 1402, 1606
ApeKI GCWGC 4 cut(s) 187, 1157, 1708, 1928
ApoI RAATTY 6 cut(s) 377, 1216, 1319, 1632, 2030, 2099
AseI ATTAAT 1 cut(s) 1688
Asp700I GAANNNNTTC 1 cut(s) 2030
AspLEI GCGC 1 cut(s) 1890
AspS9I GGNCC 7 cut(s) 121, 242, 1235, 1496, 1833, 1958, 2146
AsuC2I CCSGG 1 cut(s) 1901
AsuHPI GGTGA 6 cut(s) 16, 713, 1016, 1134, 1454, 2086
AsuII TTCGAA 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 6 cut(s) 121, 1235, 1496, 1833, 1958, 2146
BaeGI GKGCMC 2 cut(s) 1355, 1523
BalI TGGCCA 1 cut(s) 1210
BamHI GGATCC 2 cut(s) 346, 494
BanII GRGCYC 2 cut(s) 429, 2166
BauI CACGAG 2 cut(s) 1014, 1252
BbsI GAAGAC 1 cut(s) 1192
Bbv12I GWGCWC 2 cut(s) 429, 2166
BbvI GCAGC 4 cut(s) 174, 1144, 1695, 1915
BccI CCATC 4 cut(s) 178, 298, 1218, 1508
BciT130I CCWGG 4 cut(s) 374, 499, 863, 997
BclI TGATCA 1 cut(s) 1720
BcnI CCSGG 1 cut(s) 1901
BcoDI GTCTC 5 cut(s) 1176, 1299, 1771, 1779, 1916
BfaI CTAG 4 cut(s) 53, 767, 1406, 1452
BfmI CTRYAG 3 cut(s) 901, 1559, 1929
BfuAI ACCTGC 1 cut(s) 1821
BisI GCNGC 6 cut(s) 140, 188, 921, 1158, 1709, 1929
BlsI GCNGC 6 cut(s) 141, 189, 922, 1159, 1710, 1930
Bme1390I CCNGG 5 cut(s) 374, 499, 863, 997, 1901
Bme18I GGWCC 6 cut(s) 121, 1235, 1496, 1833, 1958, 2146
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 7 cut(s) 121, 242, 1235, 1496, 1833, 1958, 2146
BmiI GGNNCC 8 cut(s) 123, 260, 348, 496, 1102, 1236, 1498, 2147
BmrFI CCNGG 5 cut(s) 374, 499, 863, 997, 1901
BmsI GCATC 6 cut(s) 217, 673, 694, 1321, 1869, 1923
BpiI GAAGAC 1 cut(s) 1192
BpmI CTGGAG 1 cut(s) 1035
Bpu14I TTCGAA 1 cut(s) 442
BpuMI CCSGG 1 cut(s) 1901
BsaI GGTCTC 2 cut(s) 1176, 1299
BsaJI CCNNGG 5 cut(s) 69, 396, 861, 1127, 1852
Bsc4I CCNNNNNNNGG 2 cut(s) 953, 1952
Bse3DI GCAATG 1 cut(s) 967
BseBI CCWGG 4 cut(s) 374, 499, 863, 997
BseDI CCNNGG 5 cut(s) 69, 396, 861, 1127, 1852
BseGI GGATG 7 cut(s) 232, 574, 601, 688, 1201, 1229, 1336
BseLI CCNNNNNNNGG 2 cut(s) 953, 1952
BseMI GCAATG 1 cut(s) 967
BseMII CTCAG 3 cut(s) 144, 222, 1315
BseRI GAGGAG 3 cut(s) 237, 1287, 1661
BseSI GKGCMC 2 cut(s) 1355, 1523
BseXI GCAGC 4 cut(s) 174, 1144, 1695, 1915
BsgI GTGCAG 1 cut(s) 173
Bsh1236I CGCG 3 cut(s) 280, 834, 1890
BshFI GGCC 7 cut(s) 68, 243, 885, 920, 1210, 1404, 1608
BsiHKAI GWGCWC 2 cut(s) 429, 2166
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 2 cut(s) 886, 1900
BslFI GGGAC 2 cut(s) 434, 2159
BslI CCNNNNNNNGG 2 cut(s) 953, 1952
BsmAI GTCTC 5 cut(s) 1176, 1299, 1771, 1779, 1916
BsmFI GGGAC 2 cut(s) 434, 2159
BsmI GAATGC 2 cut(s) 969, 1752
BsnI GGCC 7 cut(s) 68, 243, 885, 920, 1210, 1404, 1608
Bso31I GGTCTC 2 cut(s) 1176, 1299
BsoBI CYCGRG 1 cut(s) 395
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 4 cut(s) 429, 1355, 1523, 2166
Bsp143I GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
Bsp19I CCATGG 1 cut(s) 1127
Bsp68I TCGCGA 1 cut(s) 280
BspACI CCGC 5 cut(s) 106, 139, 740, 921, 1682
BspANI GGCC 7 cut(s) 68, 243, 885, 920, 1210, 1404, 1608
BspCNI CTCAG 3 cut(s) 145, 221, 1314
BspFNI CGCG 3 cut(s) 280, 834, 1890
BspHI TCATGA 2 cut(s) 181, 1723
BspLI GGNNCC 8 cut(s) 123, 260, 348, 496, 1102, 1236, 1498, 2147
BspMAI CTGCAG 1 cut(s) 1933
BspMI ACCTGC 1 cut(s) 1821
BspPI GGATC 6 cut(s) 341, 354, 489, 502, 996, 1106
BspT104I TTCGAA 1 cut(s) 442
BspTNI GGTCTC 2 cut(s) 1176, 1299
BsrBI CCGCTC 1 cut(s) 923
BsrDI GCAATG 1 cut(s) 967
BssECI CCNNGG 5 cut(s) 69, 396, 861, 1127, 1852
BssMI GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
BssSI CACGAG 2 cut(s) 1014, 1252
BssT1I CCWWGG 2 cut(s) 69, 1127
Bst2BI CACGAG 2 cut(s) 1014, 1252
Bst2UI CCWGG 4 cut(s) 374, 499, 863, 997
Bst4CI ACNGT 7 cut(s) 317, 800, 905, 1357, 1392, 1804, 1968
Bst6I CTCTTC 4 cut(s) 57, 374, 1376, 2128
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 4 cut(s) 1208, 1476, 1530, 1653
BstDEI CTNAG 7 cut(s) 153, 208, 875, 1301, 1366, 1772, 2129
BstDSI CCRYGG 1 cut(s) 1127
BstF5I GGATG 7 cut(s) 232, 574, 601, 688, 1201, 1229, 1336
BstFNI CGCG 3 cut(s) 280, 834, 1890
BstHHI GCGC 1 cut(s) 1890
BstKTI GATC 8 cut(s) 201, 349, 497, 991, 1024, 1114, 1427, 1723
BstMAI GTCTC 5 cut(s) 1176, 1299, 1771, 1779, 1916
BstMBI GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
BstMWI GCNNNNNNNGC 5 cut(s) 683, 920, 966, 1484, 1577
BstNI CCWGG 4 cut(s) 374, 499, 863, 997
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 5 cut(s) 372, 497, 861, 995, 1899
BstSFI CTRYAG 3 cut(s) 901, 1559, 1929
BstSLI GKGCMC 2 cut(s) 1355, 1523
BstUI CGCG 3 cut(s) 280, 834, 1890
BstV1I GCAGC 4 cut(s) 174, 1144, 1695, 1915
BstV2I GAAGAC 1 cut(s) 1192
BstX2I RGATCY 4 cut(s) 346, 494, 988, 1111
BstYI RGATCY 4 cut(s) 346, 494, 988, 1111
BsuRI GGCC 7 cut(s) 68, 243, 885, 920, 1210, 1404, 1608
BtgI CCRYGG 1 cut(s) 1127
BtsCI GGATG 7 cut(s) 232, 574, 601, 688, 1201, 1229, 1336
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 5 cut(s) 496, 677, 1397, 1437, 1942
BtuMI TCGCGA 1 cut(s) 280
BveI ACCTGC 1 cut(s) 1821
Cac8I GCNNGC 4 cut(s) 1208, 1476, 1530, 1653
CciI TCATGA 2 cut(s) 181, 1723
CfoI GCGC 1 cut(s) 1890
Cfr13I GGNCC 7 cut(s) 121, 242, 1235, 1496, 1833, 1958, 2146
CseI GACGC 3 cut(s) 1437, 1879, 2103
Csp6I GTAC 3 cut(s) 764, 1386, 2093
CspCI CAANNNNNGTGG 2 cut(s) 1936, 1971
CviQI GTAC 3 cut(s) 764, 1386, 2093
DdeI CTNAG 7 cut(s) 153, 208, 875, 1301, 1366, 1772, 2129
DpnI GATC 8 cut(s) 200, 348, 496, 990, 1023, 1113, 1426, 1722
DpnII GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
EaeI YGGCCR 1 cut(s) 1208
Eam1104I CTCTTC 4 cut(s) 57, 374, 1376, 2128
EarI CTCTTC 4 cut(s) 57, 374, 1376, 2128
Ecl136II GAGCTC 2 cut(s) 427, 2164
Eco130I CCWWGG 2 cut(s) 69, 1127
Eco147I AGGCCT 2 cut(s) 1404, 1608
Eco24I GRGCYC 2 cut(s) 429, 2166
Eco31I GGTCTC 2 cut(s) 1176, 1299
Eco47I GGWCC 6 cut(s) 121, 1235, 1496, 1833, 1958, 2146
Eco53kI GAGCTC 2 cut(s) 427, 2164
Eco57I CTGAAG 1 cut(s) 2042
Eco88I CYCGRG 1 cut(s) 395
EcoICRI GAGCTC 2 cut(s) 427, 2164
EcoO109I RGGNCCY 1 cut(s) 242
EcoRI GAATTC 2 cut(s) 377, 1632
EcoRII CCWGG 4 cut(s) 372, 497, 861, 995
EcoT14I CCWWGG 2 cut(s) 69, 1127
EcoT38I GRGCYC 2 cut(s) 429, 2166
ErhI CCWWGG 2 cut(s) 69, 1127
FalI AAGNNNNNCTT 4 cut(s) 839, 871, 2063, 2095
FaqI GGGAC 2 cut(s) 434, 2159
FauI CCCGC 2 cut(s) 113, 1689
FauNDI CATATG 1 cut(s) 630
FbaI TGATCA 1 cut(s) 1720
FblI GTMKAC 2 cut(s) 829, 1068
Fnu4HI GCNGC 6 cut(s) 140, 188, 921, 1158, 1709, 1929
FokI GGATG 7 cut(s) 239, 581, 608, 695, 1208, 1236, 1343
FriOI GRGCYC 2 cut(s) 429, 2166
Fsp4HI GCNGC 6 cut(s) 140, 188, 921, 1158, 1709, 1929
FspBI CTAG 4 cut(s) 53, 767, 1406, 1452
GlaI GCGC 1 cut(s) 1889
GluI GCNGC 6 cut(s) 140, 188, 921, 1158, 1709, 1929
GsuI CTGGAG 1 cut(s) 1035
HaeIII GGCC 7 cut(s) 68, 243, 885, 920, 1210, 1404, 1608
HapII CCGG 2 cut(s) 886, 1900
HgaI GACGC 3 cut(s) 1437, 1879, 2103
HhaI GCGC 1 cut(s) 1890
Hin6I GCGC 1 cut(s) 1888
HinP1I GCGC 1 cut(s) 1888
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HindIII AAGCTT 3 cut(s) 1363, 1569, 1653
HinfI GANTC 6 cut(s) 84, 391, 614, 774, 1070, 1163
HpaII CCGG 2 cut(s) 886, 1900
HphI GGTGA 6 cut(s) 16, 713, 1016, 1134, 1454, 2086
Hpy166II GTNNAC 4 cut(s) 830, 1069, 1388, 1735
Hpy188I TCNGA 5 cut(s) 322, 1116, 1837, 2008, 2175
Hpy8I GTNNAC 4 cut(s) 830, 1069, 1388, 1735
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 3 cut(s) 273, 593, 1037
HpyCH4III ACNGT 7 cut(s) 317, 800, 905, 1357, 1392, 1804, 1968
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 8 cut(s) 190, 677, 686, 707, 821, 914, 1931, 1943
HpyF10VI GCNNNNNNNGC 5 cut(s) 683, 920, 966, 1484, 1577
HpyF3I CTNAG 7 cut(s) 153, 208, 875, 1301, 1366, 1772, 2129
HpySE526I ACGT 1 cut(s) 57
HspAI GCGC 1 cut(s) 1888
Ksp22I TGATCA 1 cut(s) 1720
Kzo9I GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
LmnI GCTCC 6 cut(s) 928, 957, 1577, 1648, 1694, 2175
Lsp1109I GCAGC 4 cut(s) 174, 1144, 1695, 1915
LweI GCATC 6 cut(s) 217, 673, 694, 1321, 1869, 1923
MaeI CTAG 4 cut(s) 53, 767, 1406, 1452
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 5 cut(s) 408, 1075, 1339, 1895, 2124
MalI GATC 8 cut(s) 200, 348, 496, 990, 1023, 1113, 1426, 1722
MbiI CCGCTC 1 cut(s) 923
MboI GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
MboII GAAGA 8 cut(s) 19, 74, 361, 1192, 1393, 2035, 2038, 2145
MfeI CAATTG 4 cut(s) 220, 708, 759, 951
MflI RGATCY 4 cut(s) 346, 494, 988, 1111
MhlI GDGCHC 4 cut(s) 429, 1355, 1523, 2166
MlsI TGGCCA 1 cut(s) 1210
MluI ACGCGT 1 cut(s) 832
MluNI TGGCCA 1 cut(s) 1210
MlyI GAGTC 2 cut(s) 93, 1064
MmeI TCCRAC 2 cut(s) 1786, 2142
Mox20I TGGCCA 1 cut(s) 1210
MroXI GAANNNNTTC 1 cut(s) 2030
MscI TGGCCA 1 cut(s) 1210
MseI TTAA 8 cut(s) 624, 638, 977, 1688, 1988, 2058, 2070, 2154
MslI CAYNNNNRTG 2 cut(s) 180, 725
Msp20I TGGCCA 1 cut(s) 1210
MspA1I CMGCKG 1 cut(s) 1928
MspI CCGG 2 cut(s) 886, 1900
MspR9I CCNGG 5 cut(s) 374, 499, 863, 997, 1901
MunI CAATTG 4 cut(s) 220, 708, 759, 951
Mva1269I GAATGC 2 cut(s) 969, 1752
MvaI CCWGG 4 cut(s) 374, 499, 863, 997
MvnI CGCG 3 cut(s) 280, 834, 1890
MwoI GCNNNNNNNGC 5 cut(s) 683, 920, 966, 1484, 1577
NciI CCSGG 1 cut(s) 1901
NcoI CCATGG 1 cut(s) 1127
NdeI CATATG 1 cut(s) 630
NdeII GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
NlaIV GGNNCC 8 cut(s) 123, 260, 348, 496, 1102, 1236, 1498, 2147
NmuCI GTSAC 3 cut(s) 408, 1075, 1895
NruI TCGCGA 1 cut(s) 280
NspI RCATGY 1 cut(s) 724
NspV TTCGAA 1 cut(s) 442
PagI TCATGA 2 cut(s) 181, 1723
PceI AGGCCT 2 cut(s) 1404, 1608
PctI GAATGC 2 cut(s) 969, 1752
PdmI GAANNNNTTC 1 cut(s) 2030
PfeI GAWTC 4 cut(s) 391, 614, 774, 1163
PflMI CCANNNNNTGG 1 cut(s) 953
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 6 cut(s) 141, 189, 922, 1159, 1710, 1930
PleI GAGTC 2 cut(s) 92, 1064
PpsI GAGTC 2 cut(s) 92, 1064
PshBI ATTAAT 1 cut(s) 1688
Psp124BI GAGCTC 2 cut(s) 429, 2166
Psp6I CCWGG 4 cut(s) 372, 497, 861, 995
PspGI CCWGG 4 cut(s) 372, 497, 861, 995
PspN4I GGNNCC 8 cut(s) 123, 260, 348, 496, 1102, 1236, 1498, 2147
PspPI GGNCC 7 cut(s) 121, 242, 1235, 1496, 1833, 1958, 2146
PstI CTGCAG 1 cut(s) 1933
PsuI RGATCY 4 cut(s) 346, 494, 988, 1111
PvuII CAGCTG 1 cut(s) 1928
RruI TCGCGA 1 cut(s) 280
RsaI GTAC 3 cut(s) 765, 1387, 2094
RsaNI GTAC 3 cut(s) 764, 1386, 2093
RseI CAYNNNNRTG 2 cut(s) 180, 725
SacI GAGCTC 2 cut(s) 429, 2166
SalI GTCGAC 1 cut(s) 1067
SaqAI TTAA 8 cut(s) 624, 638, 977, 1688, 1988, 2058, 2070, 2154
SatI GCNGC 6 cut(s) 140, 188, 921, 1158, 1709, 1929
Sau3AI GATC 8 cut(s) 198, 346, 494, 988, 1021, 1111, 1424, 1720
Sau96I GGNCC 7 cut(s) 121, 242, 1235, 1496, 1833, 1958, 2146
SchI GAGTC 2 cut(s) 93, 1064
ScrFI CCNGG 5 cut(s) 374, 499, 863, 997, 1901
SduI GDGCHC 4 cut(s) 429, 1355, 1523, 2166
SfaNI GCATC 6 cut(s) 217, 673, 694, 1321, 1869, 1923
SfcI CTRYAG 3 cut(s) 901, 1559, 1929
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 6 cut(s) 121, 1235, 1496, 1833, 1958, 2146
SmiMI CAYNNNNRTG 2 cut(s) 180, 725
SseBI AGGCCT 2 cut(s) 1404, 1608
SsiI CCGC 5 cut(s) 106, 139, 740, 921, 1682
SspI AATATT 2 cut(s) 523, 1084
SspMI CTAG 4 cut(s) 53, 767, 1406, 1452
SstI GAGCTC 2 cut(s) 429, 2166
StuI AGGCCT 2 cut(s) 1404, 1608
StyD4I CCNGG 5 cut(s) 372, 497, 861, 995, 1899
StyI CCWWGG 2 cut(s) 69, 1127
TaaI ACNGT 7 cut(s) 317, 800, 905, 1357, 1392, 1804, 1968
TaiI ACGT 1 cut(s) 60
TaqI TCGA 8 cut(s) 78, 442, 717, 907, 1020, 1068, 1595, 2107
TatI WGTACW 2 cut(s) 763, 1385
TauI GCSGC 2 cut(s) 142, 923
TfiI GAWTC 4 cut(s) 391, 614, 774, 1163
Tru1I TTAA 8 cut(s) 624, 638, 977, 1688, 1988, 2058, 2070, 2154
Tru9I TTAA 8 cut(s) 624, 638, 977, 1688, 1988, 2058, 2070, 2154
TscAI CASTG 5 cut(s) 496, 684, 1397, 1444, 1942
TseFI GTSAC 3 cut(s) 408, 1075, 1895
TseI GCWGC 4 cut(s) 187, 1157, 1708, 1928
Tsp45I GTSAC 3 cut(s) 408, 1075, 1895
TspGWI ACGGA 3 cut(s) 134, 1063, 1797
TspRI CASTG 5 cut(s) 496, 684, 1397, 1444, 1942
Van91I CCANNNNNTGG 1 cut(s) 953
VpaK11BI GGWCC 6 cut(s) 121, 1235, 1496, 1833, 1958, 2146
VspI ATTAAT 1 cut(s) 1688
XapI RAATTY 6 cut(s) 377, 1216, 1319, 1632, 2030, 2099
XceI RCATGY 1 cut(s) 724
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
XmiI GTMKAC 2 cut(s) 829, 1068
XmnI GAANNNNTTC 1 cut(s) 2030
XspI CTAG 4 cut(s) 53, 767, 1406, 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.