Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0016056

Physical Location & Seq

Genomic Coordinates Reverse (-)

1629132 .. 1635067

Gene Structure 5936 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1021100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGGAAGCTGGAGGGAGCAGCAAGAACCAGACGGAGCCTAAGAAACGCCAATCTAAGACGCCCAGAAAGCCCAAAGATAGCGTTCTCGAACAGAAATCGCCGGCGGAGTTCTTTGCGGAGAACAAGAACATTGCTGGGTTCGATAATCCAGGGAAATGTCTTTACACTACTGTGAGGGAACTTGTTGAGAACTCACTTGATTCCACGGAGTCCATATCGGAGCTTCCTGTGATAGAAATAACGATTGAAGATATTGAGAAAAGCAAGTTCAATTCTATGATTGGTCTTATTGATCGAGATCGTGTTGATGAGGCATTATACGATGATTATGAAACAGCTAAAGCTCGTGAGAAACGATTAGCAAAGGAAGCTCGTGCTCAAGAATTACAGGCAAAAAATGCTTCCTCTGGAAAGATGGTCAAAGAACCTCCAGCTGCAAAGACCATAAAGGGTCGAGGTGAGGCTTCATTTTACAGGGTTACATGCAAGGATAATGGAAAGGGGATGCCACATGATGACATTCCAAATATGTTTGGACGAGTGTTATCTGGAACAAAATATGGCTTGAAGCAGACGCGTGGGAAGTTTGGTCTAGGTGCAAAGATGGCATTAATTTGGTCAAAGATGAGTACAGGGCTTCCTATAGAGATCTCGTCATCTTTGAAGGGCCAAAATTATATTTCATTTTGCAGGCTGGATATAGATATTCATCGGAATATTCCTCATGTCCATTTACATGAAAAACGGGATAACAAGGTTCGGTGGCATGGAGCTGAGATCCAAGTTGTTATTGAAGGAAATTGGACAAGTTACCGTTCCAAGATATTGCATTACATGCGACAAATGGCTGTTATAACTCCTTATGCCGAGTTTCTTTTTAAATTTGTATCAGGTGCAGCAGATAAAAGCATTACCATAAGGTTTGCTCGGAGAACAGATGTGATGCCTCCAGTTCCTCTTGAGACAAAGCACCATCCATCGTCCGTTGACTTATTGCTAATAAAGCGCCTCATTGAAGAAAATCCAAAGCAAAACCTTTTACAGTTTCTTCAGCATGAATTTGTTAATATACGAAAAGCATATGCTGAACGACTAATTGGGGAATTGGGTCCTGAGTTCAGTCCAAAAATGCCTATTAAGTCTCTAACTTCTCAACAAATTGTTCGCATCCATCAGTTATTTCGTCAAGCTAAGTTTGATGATCCTAGTGGTGATTGTCTGAGTCCTGCAGGGGAATACAATCTTCGTCTAGGAATTATAAAGGAGCTGCATCCAGACATGGTTGCAACTTATTCTGGAAGTGCCCGAGTTTTTGAAGGCCACCCATTCATTGTGGAAGCTGGTGTCAGTGTGGGTGGAAAAGATGTCAAGCAAGGTTTGAATATTTTCCGATTTGCAAACCGAATTCCACTTCTTTTCGAACAAGGGGCTGATGTTGTCACCAGGACCGCTCTTAAGAGAATCAATTGGAGTAGTTACAAGATCAACCAAACTCAGGATAAGATTGGTGTCTTTGTGAGCATTGTAAGCACAAAAATACCCTTTAAAGGAACGGGGAAGGAGTATATTGGAGATGATATAACTGAAATAGCTTCAGCTGTCAAGTCTGCCCTTCAACAGTGTTGCATCCAGCTAAAAACCAAGATAGTGAAGAAAATGCAAGCTCGTGAACAGCAGGAGAGAAAGCGAAGTTTGAGCAAGTATCTTGATAATGCTTGTACTGTTTTTTATGATGTGTGGAAGGAGATCAAAGCGCAGAATTCGGAACAATCACATGCAGCAAAGAAGCAACGCGATGAAATGTACAAGGAAATATCAAATCAAACAGTTACAAAAGCAATACTCAAGGAAAAGCTTTTACAACATGTTGAACAGGTGGACTATGAGATGGCATTGGAGTACGCTACGCAAAGCGGAGTGAGTGAGGAACCCAGAGAAGCGATATTTCTGCAGTCACTTGAAGCTGCTGAAGATAGCTTTATTGATTTGCATAGTCCCACATTTGTCTTTCGGCTGTTCCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003735 GO:0003824 GO:0003916 GO:0003918 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0007389 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009330 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010467 GO:0014070 GO:0015935 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0019538 GO:0022402 GO:0022613 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0033993 GO:0034641 GO:0034645 GO:0042023 GO:0042221 GO:0042254 GO:0042623 GO:0042802 GO:0042803 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044446 GO:0044464 GO:0044786 GO:0046483 GO:0046983 GO:0048856 GO:0048869 GO:0050896 GO:0061505 GO:0071704 GO:0071840 GO:0090304 GO:0090558 GO:0140097 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HATPase_c HATPase_c_3 Topo-VIb_trans

Protein Analysis

675

Amino Acids

76.45

Weight (kDa)

9.18

Isoelectric Point (pI)

51.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c_3	PF13589	159 - 219	6.7e-06	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Topo-VIb_trans	PF09239	402 - 561	1.1e-56	Topoisomerase VI B subunit, transducer

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20780
fragaria_vesca	FvH4_3g43550
malus_domestica	MD03G1021100.v1.1
prunus_persica	Prupe.6G018600_v2.0.a1 Prupe.6G018600_v2.0.a1 Prupe.6G018600_v2.0.a1
pyrus_communis	pycom03g01820
rosa_chinensis	RchiOBHm_Chr5g0078101
rosa_laevigata	RLG00000036751
rosa_multiflora	Rmu_sc0008741.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000360 Rroxscaffold_1G00004120
rosa_rugosa	Rorug05G0458500
rosa_samantha	Rh5AG513100
rosa_wichuraiana	Rw5G047590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	854, 1259
AccBSI	CCGCTC	1 cut(s)	1451
AccII	CGCG	2 cut(s)	577, 1794
AciI	CCGC	5 cut(s)	104, 116, 1449, 1914, 2020
AclWI	GGATC	2 cut(s)	772, 1196
AcsI	RAATTY	4 cut(s)	881, 1058, 1404, 1759
AcuI	CTGAAG	3 cut(s)	1034, 1578, 1989
AcyI	GRCGYC	1 cut(s)	59
AfaI	GTAC	4 cut(s)	631, 1720, 1805, 1901
AfiI	CCNNNNNNNGG	2 cut(s)	1495, 1547
AflII	CTTAAG	1 cut(s)	1454
AflIII	ACRYGT	2 cut(s)	575, 1864
AjnI	CCWGG	2 cut(s)	148, 1442
AjuI	GAANNNNNNNTTGG	2 cut(s)	1080, 1112
AleI	CACNNNNGTG	1 cut(s)	170
Alw21I	GWGCWC	1 cut(s)	379
Alw26I	GTCTC	2 cut(s)	956, 1146
AlwI	GGATC	2 cut(s)	772, 1196
Ama87I	CYCGRG	1 cut(s)	1305
AoxI	GGCC	2 cut(s)	667, 1318
ApeKI	GCWGC	6 cut(s)	18, 434, 896, 1267, 1778, 1964
ApoI	RAATTY	4 cut(s)	881, 1058, 1404, 1759
AseI	ATTAAT	1 cut(s)	611
Asp700I	GAANNNNTTC	1 cut(s)	1385
AspLEI	GCGC	2 cut(s)	1008, 1756
AspS9I	GGNCC	3 cut(s)	667, 1109, 1446
AsuHPI	GGTGA	3 cut(s)	470, 1223, 1432
AsuII	TTCGAA	1 cut(s)	1419
AvaI	CYCGRG	1 cut(s)	1305
AvaII	GGWCC	2 cut(s)	1109, 1446
BaeGI	GKGCMC	1 cut(s)	1306
BauI	CACGAG	3 cut(s)	345, 372, 1665
Bbv12I	GWGCWC	1 cut(s)	379
BbvI	GCAGC	6 cut(s)	30, 421, 908, 1254, 1790, 1951
BccI	CCATC	6 cut(s)	409, 598, 981, 985, 1179, 1882
BcgI	CGANNNNNNTGC	2 cut(s)	1930, 1964
BciT130I	CCWGG	2 cut(s)	150, 1444
BcoDI	GTCTC	2 cut(s)	956, 1146
BfaI	CTAG	4 cut(s)	593, 1206, 1250, 2023
BfmI	CTRYAG	3 cut(s)	642, 1227, 1949
BfoI	RGCGCY	1 cut(s)	1009
BfrI	CTTAAG	1 cut(s)	1454
BglII	AGATCT	1 cut(s)	648
BisI	GCNGC	6 cut(s)	19, 435, 897, 1268, 1779, 1965
BlsI	GCNGC	6 cut(s)	20, 436, 898, 1269, 1780, 1966
Bme1390I	CCNGG	2 cut(s)	150, 1444
Bme18I	GGWCC	2 cut(s)	1109, 1446
BmeT110I	CYCGRG	1 cut(s)	1305
BmgT120I	GGNCC	3 cut(s)	667, 1109, 1446
BmiI	GGNNCC	3 cut(s)	36, 1110, 1929
BmrFI	CCNGG	2 cut(s)	150, 1444
BmsI	GCATC	5 cut(s)	495, 933, 1176, 1279, 1635
BpmI	CTGGAG	3 cut(s)	30, 414, 933
Bpu14I	TTCGAA	1 cut(s)	1419
BpuEI	CTTGAG	3 cut(s)	363, 980, 1829
BsaBI	GATNNNNATC	2 cut(s)	297, 708
BsaHI	GRCGYC	1 cut(s)	59
BsaJI	CCNNGG	2 cut(s)	149, 204
Bsc4I	CCNNNNNNNGG	2 cut(s)	1495, 1547
Bse118I	RCCGGY	1 cut(s)	100
Bse1I	ACTGG	1 cut(s)	950
Bse3DI	GCAATG	1 cut(s)	129
Bse8I	GATNNNNATC	2 cut(s)	297, 708
BseBI	CCWGG	2 cut(s)	150, 1444
BseDI	CCNNGG	2 cut(s)	149, 204
BseGI	GGATG	5 cut(s)	510, 973, 1167, 1270, 1626
BseJI	GATNNNNATC	2 cut(s)	297, 708
BseLI	CCNNNNNNNGG	2 cut(s)	1495, 1547
BseMI	GCAATG	1 cut(s)	129
BseMII	CTCAG	4 cut(s)	765, 1104, 1211, 1508
BseNI	ACTGG	1 cut(s)	950
BseSI	GKGCMC	1 cut(s)	1306
BseXI	GCAGC	6 cut(s)	30, 421, 908, 1254, 1790, 1951
BseYI	CCCAGC	1 cut(s)	134
BsgI	GTGCAG	1 cut(s)	915
Bsh1236I	CGCG	2 cut(s)	577, 1794
BshFI	GGCC	2 cut(s)	669, 1320
BsiHKAI	GWGCWC	1 cut(s)	379
BsiHKCI	CYCGRG	1 cut(s)	1305
BsiSI	CCGG	1 cut(s)	101
BslFI	GGGAC	1 cut(s)	1980
BslI	CCNNNNNNNGG	2 cut(s)	1495, 1547
BsmAI	GTCTC	2 cut(s)	956, 1146
BsmFI	GGGAC	1 cut(s)	1980
BsnI	GGCC	2 cut(s)	669, 1320
BsoBI	CYCGRG	1 cut(s)	1305
Bsp119I	TTCGAA	1 cut(s)	1419
Bsp1286I	GDGCHC	2 cut(s)	379, 1306
Bsp1407I	TGTACA	1 cut(s)	1803
Bsp143I	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
BspACI	CCGC	5 cut(s)	104, 116, 1449, 1914, 2020
BspANI	GGCC	2 cut(s)	669, 1320
BspCNI	CTCAG	4 cut(s)	766, 1105, 1212, 1507
BspFNI	CGCG	2 cut(s)	577, 1794
BspLI	GGNNCC	3 cut(s)	36, 1110, 1929
BspMAI	CTGCAG	2 cut(s)	1231, 1953
BspPI	GGATC	2 cut(s)	772, 1196
BspT104I	TTCGAA	1 cut(s)	1419
BspTI	CTTAAG	1 cut(s)	1454
BsrBI	CCGCTC	1 cut(s)	1451
BsrDI	GCAATG	1 cut(s)	129
BsrFI	RCCGGY	1 cut(s)	100
BsrGI	TGTACA	1 cut(s)	1803
BsrI	ACTGG	1 cut(s)	950
BssAI	RCCGGY	1 cut(s)	100
BssECI	CCNNGG	2 cut(s)	149, 204
BssMI	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	3 cut(s)	345, 372, 1665
Bst2BI	CACGAG	3 cut(s)	345, 372, 1665
Bst2UI	CCWGG	2 cut(s)	150, 1444
Bst4CI	ACNGT	6 cut(s)	172, 815, 1044, 1620, 1723, 1828
BstACI	GRCGYC	1 cut(s)	59
BstAFI	CTTAAG	1 cut(s)	1454
BstAPI	GCANNNNNTGC	2 cut(s)	398, 835
BstAUI	TGTACA	1 cut(s)	1803
BstBI	TTCGAA	1 cut(s)	1419
BstC8I	GCNNGC	3 cut(s)	102, 692, 1662
BstDEI	CTNAG	7 cut(s)	39, 54, 774, 1113, 1191, 1220, 1494
BstDSI	CCRYGG	1 cut(s)	204
BstF5I	GGATG	5 cut(s)	510, 973, 1167, 1270, 1626
BstFNI	CGCG	2 cut(s)	577, 1794
BstH2I	RGCGCY	1 cut(s)	1009
BstHHI	GCGC	2 cut(s)	1008, 1756
BstKTI	GATC	7 cut(s)	295, 301, 651, 780, 1204, 1485, 1749
BstMAI	GTCTC	2 cut(s)	956, 1146
BstMBI	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
BstMWI	GCNNNNNNNGC	7 cut(s)	67, 368, 398, 605, 835, 1003, 1527
BstNI	CCWGG	2 cut(s)	150, 1444
BstNSI	RCATGY	4 cut(s)	486, 838, 1778, 1868
BstSCI	CCNGG	2 cut(s)	148, 1442
BstSFI	CTRYAG	3 cut(s)	642, 1227, 1949
BstSLI	GKGCMC	1 cut(s)	1306
BstUI	CGCG	2 cut(s)	577, 1794
BstV1I	GCAGC	6 cut(s)	30, 421, 908, 1254, 1790, 1951
BstX2I	RGATCY	2 cut(s)	648, 777
BstYI	RGATCY	2 cut(s)	648, 777
BsuRI	GGCC	2 cut(s)	669, 1320
BtgI	CCRYGG	1 cut(s)	204
BtgZI	GCGATG	1 cut(s)	1809
BtsCI	GGATG	5 cut(s)	510, 973, 1167, 1270, 1626
BtsIMutI	CAGTG	2 cut(s)	1354, 1625
Cac8I	GCNNGC	3 cut(s)	102, 692, 1662
CfoI	GCGC	2 cut(s)	1008, 1756
Cfr10I	RCCGGY	1 cut(s)	100
Cfr13I	GGNCC	3 cut(s)	667, 1109, 1446
CseI	GACGC	2 cut(s)	67, 583
Csp6I	GTAC	4 cut(s)	630, 1719, 1804, 1900
CviQI	GTAC	4 cut(s)	630, 1719, 1804, 1900
DdeI	CTNAG	7 cut(s)	39, 54, 774, 1113, 1191, 1220, 1494
DpnI	GATC	7 cut(s)	294, 300, 650, 779, 1203, 1484, 1748
DpnII	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
DraI	TTTAAA	2 cut(s)	880, 1546
EciI	GGCGGA	1 cut(s)	119
Eco47I	GGWCC	2 cut(s)	1109, 1446
Eco57I	CTGAAG	3 cut(s)	1034, 1578, 1989
Eco88I	CYCGRG	1 cut(s)	1305
EcoO109I	RGGNCCY	1 cut(s)	1109
EcoRI	GAATTC	2 cut(s)	1404, 1759
EcoRII	CCWGG	2 cut(s)	148, 1442
FaqI	GGGAC	1 cut(s)	1980
FauNDI	CATATG	1 cut(s)	1081
Fnu4HI	GCNGC	6 cut(s)	19, 435, 897, 1268, 1779, 1965
FokI	GGATG	5 cut(s)	517, 960, 1154, 1257, 1613
Fsp4HI	GCNGC	6 cut(s)	19, 435, 897, 1268, 1779, 1965
FspBI	CTAG	4 cut(s)	593, 1206, 1250, 2023
GlaI	GCGC	2 cut(s)	1007, 1755
GluI	GCNGC	6 cut(s)	19, 435, 897, 1268, 1779, 1965
GsaI	CCCAGC	1 cut(s)	138
GsuI	CTGGAG	3 cut(s)	30, 414, 933
HaeII	RGCGCY	1 cut(s)	1009
HaeIII	GGCC	2 cut(s)	669, 1320
HapII	CCGG	1 cut(s)	101
HgaI	GACGC	2 cut(s)	67, 583
HhaI	GCGC	2 cut(s)	1008, 1756
Hin1I	GRCGYC	1 cut(s)	59
Hin6I	GCGC	2 cut(s)	1006, 1754
HinP1I	GCGC	2 cut(s)	1006, 1754
HincII	GTYRAC	1 cut(s)	988
HindII	GTYRAC	1 cut(s)	988
HindIII	AAGCTT	1 cut(s)	1853
HinfI	GANTC	4 cut(s)	200, 209, 1222, 1461
HpaII	CCGG	1 cut(s)	101
HphI	GGTGA	3 cut(s)	470, 1223, 1432
Hpy166II	GTNNAC	3 cut(s)	988, 1670, 1879
Hpy188I	TCNGA	6 cut(s)	220, 714, 930, 1221, 1391, 1765
Hpy8I	GTNNAC	3 cut(s)	988, 1670, 1879
HpyAV	CCTTC	6 cut(s)	658, 788, 1310, 1552, 1622, 1735
HpyCH4III	ACNGT	6 cut(s)	172, 815, 1044, 1620, 1723, 1828
HpyF10VI	GCNNNNNNNGC	7 cut(s)	67, 368, 398, 605, 835, 1003, 1527
HpyF3I	CTNAG	7 cut(s)	39, 54, 774, 1113, 1191, 1220, 1494
Hsp92I	GRCGYC	1 cut(s)	59
HspAI	GCGC	2 cut(s)	1006, 1754
KroI	GCCGGC	1 cut(s)	100
KroNI	GCCGGC	1 cut(s)	102
Kzo9I	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
LmnI	GCTCC	5 cut(s)	15, 34, 220, 770, 1264
Lsp1109I	GCAGC	6 cut(s)	30, 421, 908, 1254, 1790, 1951
LweI	GCATC	5 cut(s)	495, 933, 1176, 1279, 1635
MaeI	CTAG	4 cut(s)	593, 1206, 1250, 2023
MaeIII	GTNAC	6 cut(s)	478, 809, 1438, 1475, 1828, 1953
MalI	GATC	7 cut(s)	294, 300, 650, 779, 1203, 1484, 1748
MbiI	CCGCTC	1 cut(s)	1451
MboI	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
MboII	GAAGA	6 cut(s)	260, 1028, 1040, 1235, 1663, 1982
MfeI	CAATTG	1 cut(s)	1465
MflI	RGATCY	2 cut(s)	648, 777
MhlI	GDGCHC	2 cut(s)	379, 1306
MluI	ACGCGT	1 cut(s)	575
MlyI	GAGTC	2 cut(s)	218, 1231
MreI	CGCCGGCG	1 cut(s)	100
MroNI	GCCGGC	1 cut(s)	100
MroXI	GAANNNNTTC	1 cut(s)	1385
MseI	TTAA	6 cut(s)	611, 879, 1065, 1137, 1455, 1545
MslI	CAYNNNNRTG	2 cut(s)	170, 735
MspA1I	CMGCKG	2 cut(s)	434, 1598
MspCI	CTTAAG	1 cut(s)	1454
MspI	CCGG	1 cut(s)	101
MspR9I	CCNGG	2 cut(s)	150, 1444
MunI	CAATTG	1 cut(s)	1465
MvaI	CCWGG	2 cut(s)	150, 1444
MvnI	CGCG	2 cut(s)	577, 1794
MwoI	GCNNNNNNNGC	7 cut(s)	67, 368, 398, 605, 835, 1003, 1527
NaeI	GCCGGC	1 cut(s)	102
NdeI	CATATG	1 cut(s)	1081
NdeII	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
NgoMIV	GCCGGC	1 cut(s)	100
NlaIV	GGNNCC	3 cut(s)	36, 1110, 1929
NmeAIII	GCCGAG	1 cut(s)	892
NmuCI	GTSAC	2 cut(s)	1438, 1953
NspI	RCATGY	4 cut(s)	486, 838, 1778, 1868
NspV	TTCGAA	1 cut(s)	1419
OliI	CACNNNNGTG	1 cut(s)	170
PciI	ACATGT	1 cut(s)	1864
PcsI	WCGNNNNNNNCGW	1 cut(s)	352
PdiI	GCCGGC	1 cut(s)	102
PdmI	GAANNNNTTC	1 cut(s)	1385
PfeI	GAWTC	2 cut(s)	200, 1461
PkrI	GCNGC	6 cut(s)	20, 436, 898, 1269, 1780, 1966
PleI	GAGTC	2 cut(s)	217, 1230
PpsI	GAGTC	2 cut(s)	217, 1230
PpuMI	RGGWCCY	1 cut(s)	1109
PscI	ACATGT	1 cut(s)	1864
PshBI	ATTAAT	1 cut(s)	611
PsiI	TTATAA	2 cut(s)	854, 1259
Psp5II	RGGWCCY	1 cut(s)	1109
Psp6I	CCWGG	2 cut(s)	148, 1442
PspFI	CCCAGC	1 cut(s)	134
PspGI	CCWGG	2 cut(s)	148, 1442
PspN4I	GGNNCC	3 cut(s)	36, 1110, 1929
PspPI	GGNCC	3 cut(s)	667, 1109, 1446
PspPPI	RGGWCCY	1 cut(s)	1109
PstI	CTGCAG	2 cut(s)	1231, 1953
PsuI	RGATCY	2 cut(s)	648, 777
PvuII	CAGCTG	2 cut(s)	434, 1598
RsaI	GTAC	4 cut(s)	631, 1720, 1805, 1901
RsaNI	GTAC	4 cut(s)	630, 1719, 1804, 1900
RseI	CAYNNNNRTG	2 cut(s)	170, 735
SaqAI	TTAA	6 cut(s)	611, 879, 1065, 1137, 1455, 1545
SatI	GCNGC	6 cut(s)	19, 435, 897, 1268, 1779, 1965
Sau3AI	GATC	7 cut(s)	292, 298, 648, 777, 1201, 1482, 1746
Sau96I	GGNCC	3 cut(s)	667, 1109, 1446
SbfI	CCTGCAGG	1 cut(s)	1231
SchI	GAGTC	2 cut(s)	218, 1231
ScrFI	CCNGG	2 cut(s)	150, 1444
SdaI	CCTGCAGG	1 cut(s)	1231
SduI	GDGCHC	2 cut(s)	379, 1306
SfaNI	GCATC	5 cut(s)	495, 933, 1176, 1279, 1635
SfcI	CTRYAG	3 cut(s)	642, 1227, 1949
SfuI	TTCGAA	1 cut(s)	1419
SgrAI	CRCCGGYG	1 cut(s)	100
SinI	GGWCC	2 cut(s)	1109, 1446
SmiMI	CAYNNNNRTG	2 cut(s)	170, 735
SmlI	CTYRAG	4 cut(s)	378, 959, 1454, 1844
SmoI	CTYRAG	4 cut(s)	378, 959, 1454, 1844
Sse8387I	CCTGCAGG	1 cut(s)	1231
SsiI	CCGC	5 cut(s)	104, 116, 1449, 1914, 2020
SspI	AATATT	2 cut(s)	718, 1384
SspMI	CTAG	4 cut(s)	593, 1206, 1250, 2023
StyD4I	CCNGG	2 cut(s)	148, 1442
TaaI	ACNGT	6 cut(s)	172, 815, 1044, 1620, 1723, 1828
TaqI	TCGA	5 cut(s)	87, 141, 295, 454, 1419
TatI	WGTACW	3 cut(s)	629, 1718, 1803
TfiI	GAWTC	2 cut(s)	200, 1461
Tru1I	TTAA	6 cut(s)	611, 879, 1065, 1137, 1455, 1545
Tru9I	TTAA	6 cut(s)	611, 879, 1065, 1137, 1455, 1545
TscAI	CASTG	2 cut(s)	1354, 1625
TseFI	GTSAC	2 cut(s)	1438, 1953
TseI	GCWGC	6 cut(s)	18, 434, 896, 1267, 1778, 1964
Tsp45I	GTSAC	2 cut(s)	1438, 1953
TspDTI	ATGAA	8 cut(s)	345, 456, 672, 698, 753, 1071, 1318, 1812
TspGWI	ACGGA	3 cut(s)	47, 221, 973
TspRI	CASTG	2 cut(s)	1354, 1625
Vha464I	CTTAAG	1 cut(s)	1454
VpaK11BI	GGWCC	2 cut(s)	1109, 1446
VspI	ATTAAT	1 cut(s)	611
XapI	RAATTY	4 cut(s)	881, 1058, 1404, 1759
XceI	RCATGY	4 cut(s)	486, 838, 1778, 1868
XmnI	GAANNNNTTC	1 cut(s)	1385
XspI	CTAG	4 cut(s)	593, 1206, 1250, 2023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.