Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0016056

Physical Location & Seq

Genomic Coordinates Forward (+)

84145537 .. 84151189

Gene Structure 5653 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000036751

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2001 bp

ATGGAGAGTAGTAAGAAACCCAAATCAAAGACTCCAGCAAAGAAGCCCAAAGACAGTGTTCTTGAGCAGAAATCTCCGGCTGAGTTCTTCGCTGAGAACAAGAACATTGCTGGATTTGATAATCCAGGGAAATGTCTTTATACTACTGTGAGGGAACTTGTGGAGAACTCACTCGATTCGACGGAGTCCATATCAGAGCTTCCTCTGATAGAAATCACAATTGAAGATATTGAGAAAAGCAAGTTCAATTCTATGATTGGTCTTGTTGATCGAGACCGTGTTGATGAGGAACTGTATGATGATCATGAAACGGCTAAGGCTCGTGAGAAACGATTAGCAAAGGAAGCTCAAGCCCAAGAATTACAAGCAAAGAATGCTTCCCTTGGAAAGAAAATCAAAGAGCCTGCAGCTCCAAAGACCATGAAGGGTCGAGGTGGGGCTGCATTTTACAGGGTTACTTGCAAGGATAATGGACGAGGAATGCCACATGATGACATTCCAAATATGTTTGGACGAGTACTGTCTGGGACAAAATATGGCTTGAAGCAGACACGTGGAAAGTTTGGTCTAGGTGCAAAGATGGCATTAATTTGGTCGAAGATGAGTACAGGGCTTCCTATAGATATCTCTTCATCAATGAAGGGCCAAAGTTATAATTCTTTCTGCAGACTGGATATAGATATTCATCGGAACATTCCTCATGTTCATATACATGAAAAACGGGATAACAAGGATAGGTGGCATGGTGCTGAAATCCAAGTTGTTATTGAGGGAAATTGGACAACTTACCGTTCCAAGATATTGCATTACATGCGACAAATGGCTGTTATCACTCCTTATGCTGAGTTCATTTTTAAATTTGTTTCAGATGTCTCTGATAAAAATGTCACCATAAGGTTTGCACGAAGAACTGATGTAATGCCTCCTGTTCCTCTTGAGACAAAGCACCATCCCTCATCTGTTGACTTATTGCTAATCAAACGCCTCATTGAAGAAACTTCAAAGCAGAACCTTTTACAGTTTCTTCAGCATGAATTCGTTAATATAGGAAAATCCTATGCTGAACGACTAATTGGGGAATTGGGTCCTGAGTTCAGTCCGAAGATGCCTGTGAAATCTCTAACTTCTCAGCAAATTGTACGCATCCATCAGTTATTTCGTCAAGCCAAGTTTAATGATCCTAGTGGTGATTGCCTGAGTCCTGCAGGGGAATACAATCTCCGTCTAGGAATTATAAAGGAGCTGCATCCGGAGTTGGTTGCTACTTATTCTGGAAGTGCTCAAGTTTTTGAAGGCCACCCATTCATTGTGGAAGCTGGTGTCAGTGTTGGTGGAAAAGATGTCAAACAAGGTTTGAATATTTTTCGATTTGCAAATCGGATTCCACTTCTTTTTGAGCAAGGTGCTGATGTTGTCACAAGGACCACCCTTAAGAGAATCAATTGGAGTAGTTACAAGATCAACCAGACTCAGGATAAAGTTGGTGTTTTTGTGAGTATTGTAAGCACAAAGATTCCCTTTAAAGGAACTGGGAAGGAGTATATTGGAGATGATATAACTGAAATAGCTTCTGCTGTCAAGTCTGCCATTCAACACTGTTGCATTCAGCTGAAAACCAAGATAGTGAAGAATATCCAAGCTCGTGAACAAAAAGAGAGAAAACGGAATTTGAGCAAGTATGCTGAAAATGTTGCTGGTGCTGTATGTGGTGTACTGAAGCAAATGCAACAAATACAAGCATCAAAGAAGAGACGTTATGATGGCAAGGAAGCAGAAATACTTAAGAAATTGTCATCTGGGATGATTACAAAAGAAATGGTCAAGGAAAAGCTTGTACAACATGTTGAACAGGTGGACTCGGAGATGGCAATGGAGTTTGCGGCAAATAGTGGAGTGAGCGAGGAACCCAGAGAATGTATATATCTTCAATCATTAGAAGCTGCTGAAGATAACTTTATCGACTTGCATAGTCCCCTATTTGTCTTTCGACTGTTCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003735 GO:0003824 GO:0003916 GO:0003918 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0007389 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009330 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010467 GO:0014070 GO:0015935 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0019538 GO:0022402 GO:0022613 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0033993 GO:0034641 GO:0034645 GO:0042023 GO:0042221 GO:0042254 GO:0042623 GO:0042802 GO:0042803 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044446 GO:0044464 GO:0044786 GO:0046483 GO:0046983 GO:0048856 GO:0048869 GO:0050896 GO:0061505 GO:0071704 GO:0071840 GO:0090304 GO:0090558 GO:0140097 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HATPase_c HATPase_c_3 Topo-VIb_trans

Protein Analysis

667

Amino Acids

75.13

Weight (kDa)

9.14

Isoelectric Point (pI)

47.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topo-VIb_trans	PF09239	394 - 553	1.6e-55	Topoisomerase VI B subunit, transducer

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20780
fragaria_vesca	FvH4_3g43550
malus_domestica	MD03G1021100.v1.1
prunus_persica	Prupe.6G018600_v2.0.a1 Prupe.6G018600_v2.0.a1 Prupe.6G018600_v2.0.a1
pyrus_communis	pycom03g01820
rosa_chinensis	RchiOBHm_Chr5g0078101
rosa_laevigata	RLG00000036751
rosa_multiflora	Rmu_sc0008741.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000360 Rroxscaffold_1G00004120
rosa_rugosa	Rorug05G0458500
rosa_samantha	Rh5AG513100
rosa_wichuraiana	Rw5G047590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	654, 1235
AccIII	TCCGGA	1 cut(s)	1249
AciI	CCGC	1 cut(s)	1880
AclWI	GGATC	1 cut(s)	1172
AcsI	RAATTY	3 cut(s)	857, 1034, 1666
AcuI	CTGAAG	3 cut(s)	1010, 1736, 1965
AcvI	CACGTG	1 cut(s)	554
AfaI	GTAC	5 cut(s)	519, 607, 1140, 1713, 1836
AfiI	CCNNNNNNNGG	2 cut(s)	1471, 1523
AflII	CTTAAG	2 cut(s)	1430, 1781
AflIII	ACRYGT	2 cut(s)	551, 1840
AjnI	CCWGG	1 cut(s)	124
AjuI	GAANNNNNNNTTGG	2 cut(s)	1056, 1088
Alw21I	GWGCWC	1 cut(s)	1282
Alw26I	GTCTC	4 cut(s)	267, 877, 932, 1744
AlwI	GGATC	1 cut(s)	1172
Aor13HI	TCCGGA	1 cut(s)	1249
AoxI	GGCC	2 cut(s)	643, 1294
ApeKI	GCWGC	4 cut(s)	407, 440, 1243, 1940
ApoI	RAATTY	3 cut(s)	857, 1034, 1666
AseI	ATTAAT	1 cut(s)	587
AspS9I	GGNCC	3 cut(s)	643, 1085, 1422
AsuHPI	GGTGA	2 cut(s)	880, 1199
AvaII	GGWCC	2 cut(s)	1085, 1422
BarI	GAAGNNNNNNTAC	2 cut(s)	1761, 1793
BauI	CACGAG	2 cut(s)	321, 1641
BbrPI	CACGTG	1 cut(s)	554
Bbv12I	GWGCWC	1 cut(s)	1282
BbvI	GCAGC	4 cut(s)	419, 427, 1230, 1927
BccI	CCATC	5 cut(s)	574, 957, 1155, 1754, 1858
BceAI	ACGGC	1 cut(s)	327
BcgI	CGANNNNNNTGC	1 cut(s)	1977
BciT130I	CCWGG	1 cut(s)	126
BclI	TGATCA	1 cut(s)	301
BcoDI	GTCTC	4 cut(s)	267, 877, 932, 1744
BfaI	CTAG	4 cut(s)	569, 1182, 1226, 1999
BfmI	CTRYAG	4 cut(s)	405, 618, 664, 1203
BfrI	CTTAAG	2 cut(s)	1430, 1781
BisI	GCNGC	5 cut(s)	408, 441, 1244, 1881, 1941
BlsI	GCNGC	5 cut(s)	409, 442, 1245, 1882, 1942
BmcAI	AGTACT	1 cut(s)	519
Bme1390I	CCNGG	1 cut(s)	126
Bme18I	GGWCC	2 cut(s)	1085, 1422
BmgT120I	GGNCC	3 cut(s)	643, 1085, 1422
BmiI	GGNNCC	2 cut(s)	1086, 1905
BmrFI	CCNGG	1 cut(s)	126
BmrI	ACTGGG	1 cut(s)	1539
BmsI	GCATC	4 cut(s)	1095, 1152, 1255, 1748
BmuI	ACTGGG	1 cut(s)	1539
BpmI	CTGGAG	1 cut(s)	18
Bpu10I	CCTNAGC	1 cut(s)	315
BpuEI	CTTGAG	4 cut(s)	83, 333, 956, 1266
BsaAI	YACGTR	1 cut(s)	554
BsaBI	GATNNNNATC	2 cut(s)	212, 684
BsaI	GGTCTC	1 cut(s)	267
BsaJI	CCNNGG	2 cut(s)	125, 382
BsaWI	WCCGGW	1 cut(s)	1249
Bsc4I	CCNNNNNNNGG	2 cut(s)	1471, 1523
Bse1I	ACTGG	2 cut(s)	675, 1534
Bse3DI	GCAATG	2 cut(s)	105, 1875
Bse8I	GATNNNNATC	2 cut(s)	212, 684
BseAI	TCCGGA	1 cut(s)	1249
BseBI	CCWGG	1 cut(s)	126
BseDI	CCNNGG	2 cut(s)	125, 382
BseGI	GGATG	4 cut(s)	949, 1143, 1246, 1806
BseJI	GATNNNNATC	2 cut(s)	212, 684
BseLI	CCNNNNNNNGG	2 cut(s)	1471, 1523
BseMI	GCAATG	2 cut(s)	105, 1875
BseMII	CTCAG	7 cut(s)	72, 84, 834, 1080, 1142, 1187, 1484
BseNI	ACTGG	2 cut(s)	675, 1534
BseXI	GCAGC	4 cut(s)	419, 427, 1230, 1927
BshFI	GGCC	2 cut(s)	645, 1296
BsiHKAI	GWGCWC	1 cut(s)	1282
BsiSI	CCGG	2 cut(s)	77, 1250
BslFI	GGGAC	2 cut(s)	541, 1956
BslI	CCNNNNNNNGG	2 cut(s)	1471, 1523
BsmAI	GTCTC	4 cut(s)	267, 877, 932, 1744
BsmBI	CGTCTC	1 cut(s)	1744
BsmFI	GGGAC	2 cut(s)	541, 1956
BsmI	GAATGC	3 cut(s)	379, 486, 1602
BsnI	GGCC	2 cut(s)	645, 1296
Bso31I	GGTCTC	1 cut(s)	267
Bsp1286I	GDGCHC	1 cut(s)	1282
Bsp13I	TCCGGA	1 cut(s)	1249
Bsp1407I	TGTACA	1 cut(s)	1834
Bsp143I	GATC	4 cut(s)	268, 301, 1177, 1458
BspACI	CCGC	1 cut(s)	1880
BspANI	GGCC	2 cut(s)	645, 1296
BspCNI	CTCAG	7 cut(s)	73, 85, 835, 1081, 1141, 1188, 1483
BspEI	TCCGGA	1 cut(s)	1249
BspHI	TCATGA	1 cut(s)	304
BspLI	GGNNCC	2 cut(s)	1086, 1905
BspMAI	CTGCAG	3 cut(s)	409, 668, 1207
BspPI	GGATC	1 cut(s)	1172
BspTI	CTTAAG	2 cut(s)	1430, 1781
BspTNI	GGTCTC	1 cut(s)	267
BsrDI	GCAATG	2 cut(s)	105, 1875
BsrGI	TGTACA	1 cut(s)	1834
BsrI	ACTGG	2 cut(s)	675, 1534
BssECI	CCNNGG	2 cut(s)	125, 382
BssMI	GATC	4 cut(s)	268, 301, 1177, 1458
BssSI	CACGAG	2 cut(s)	321, 1641
BssT1I	CCWWGG	1 cut(s)	382
Bst2BI	CACGAG	2 cut(s)	321, 1641
Bst2UI	CCWGG	1 cut(s)	126
Bst4CI	ACNGT	9 cut(s)	56, 148, 278, 294, 522, 791, 1020, 1598, 1992
Bst6I	CTCTTC	2 cut(s)	634, 1742
BstAFI	CTTAAG	2 cut(s)	1430, 1781
BstAPI	GCANNNNNTGC	2 cut(s)	374, 811
BstAUI	TGTACA	1 cut(s)	1834
BstBAI	YACGTR	1 cut(s)	554
BstC8I	GCNNGC	1 cut(s)	405
BstDEI	CTNAG	8 cut(s)	81, 93, 315, 843, 1089, 1128, 1196, 1470
BstF5I	GGATG	4 cut(s)	949, 1143, 1246, 1806
BstKTI	GATC	4 cut(s)	271, 304, 1180, 1461
BstMAI	GTCTC	4 cut(s)	267, 877, 932, 1744
BstMBI	GATC	4 cut(s)	268, 301, 1177, 1458
BstMWI	GCNNNNNNNGC	4 cut(s)	344, 374, 581, 811
BstNI	CCWGG	1 cut(s)	126
BstNSI	RCATGY	2 cut(s)	814, 1844
BstSCI	CCNGG	1 cut(s)	124
BstSFI	CTRYAG	4 cut(s)	405, 618, 664, 1203
BstV1I	GCAGC	4 cut(s)	419, 427, 1230, 1927
BsuRI	GGCC	2 cut(s)	645, 1296
BtsCI	GGATG	4 cut(s)	949, 1143, 1246, 1806
BtsIMutI	CAGTG	3 cut(s)	61, 1330, 1594
Cac8I	GCNNGC	1 cut(s)	405
CciI	TCATGA	1 cut(s)	304
Cfr13I	GGNCC	3 cut(s)	643, 1085, 1422
Csp6I	GTAC	5 cut(s)	518, 606, 1139, 1712, 1835
CviAII	CATG	9 cut(s)	305, 421, 488, 701, 713, 743, 811, 1031, 1841
CviQI	GTAC	5 cut(s)	518, 606, 1139, 1712, 1835
DdeI	CTNAG	8 cut(s)	81, 93, 315, 843, 1089, 1128, 1196, 1470
DpnI	GATC	4 cut(s)	270, 303, 1179, 1460
DpnII	GATC	4 cut(s)	268, 301, 1177, 1458
DraI	TTTAAA	2 cut(s)	856, 1522
Eam1104I	CTCTTC	2 cut(s)	634, 1742
EarI	CTCTTC	2 cut(s)	634, 1742
Eco130I	CCWWGG	1 cut(s)	382
Eco31I	GGTCTC	1 cut(s)	267
Eco32I	GATATC	1 cut(s)	625
Eco47I	GGWCC	2 cut(s)	1085, 1422
Eco57I	CTGAAG	3 cut(s)	1010, 1736, 1965
Eco72I	CACGTG	1 cut(s)	554
EcoO109I	RGGNCCY	1 cut(s)	1085
EcoRI	GAATTC	1 cut(s)	1034
EcoRII	CCWGG	1 cut(s)	124
EcoRV	GATATC	1 cut(s)	625
EcoT14I	CCWWGG	1 cut(s)	382
ErhI	CCWWGG	1 cut(s)	382
Esp3I	CGTCTC	1 cut(s)	1744
FaeI	CATG	9 cut(s)	308, 424, 491, 704, 716, 746, 814, 1034, 1844
FalI	AAGNNNNNCTT	2 cut(s)	1502, 1534
FaqI	GGGAC	2 cut(s)	541, 1956
FatI	CATG	9 cut(s)	304, 420, 487, 700, 712, 742, 810, 1030, 1840
FbaI	TGATCA	1 cut(s)	301
Fnu4HI	GCNGC	5 cut(s)	408, 441, 1244, 1881, 1941
FokI	GGATG	4 cut(s)	936, 1130, 1233, 1813
Fsp4HI	GCNGC	5 cut(s)	408, 441, 1244, 1881, 1941
FspBI	CTAG	4 cut(s)	569, 1182, 1226, 1999
GluI	GCNGC	5 cut(s)	408, 441, 1244, 1881, 1941
GsuI	CTGGAG	1 cut(s)	18
HaeIII	GGCC	2 cut(s)	645, 1296
HapII	CCGG	2 cut(s)	77, 1250
Hin1II	CATG	9 cut(s)	308, 424, 491, 704, 716, 746, 814, 1034, 1844
HincII	GTYRAC	1 cut(s)	964
HindII	GTYRAC	1 cut(s)	964
HindIII	AAGCTT	1 cut(s)	1829
HinfI	GANTC	9 cut(s)	31, 176, 185, 1198, 1381, 1437, 1468, 1513, 1856
HpaII	CCGG	2 cut(s)	77, 1250
HphI	GGTGA	2 cut(s)	880, 1199
Hpy166II	GTNNAC	4 cut(s)	964, 1646, 1712, 1855
Hpy188I	TCNGA	8 cut(s)	196, 207, 690, 868, 877, 1101, 1380, 1861
Hpy8I	GTNNAC	4 cut(s)	964, 1646, 1712, 1855
Hpy99I	CGWCG	1 cut(s)	184
HpyAV	CCTTC	4 cut(s)	418, 634, 1286, 1528
HpyCH4III	ACNGT	9 cut(s)	56, 148, 278, 294, 522, 791, 1020, 1598, 1992
HpyCH4IV	ACGT	2 cut(s)	553, 1753
HpyF10VI	GCNNNNNNNGC	4 cut(s)	344, 374, 581, 811
HpyF3I	CTNAG	8 cut(s)	81, 93, 315, 843, 1089, 1128, 1196, 1470
HpySE526I	ACGT	2 cut(s)	553, 1753
Hsp92II	CATG	9 cut(s)	308, 424, 491, 704, 716, 746, 814, 1034, 1844
Kpn2I	TCCGGA	1 cut(s)	1249
Ksp22I	TGATCA	1 cut(s)	301
Kzo9I	GATC	4 cut(s)	268, 301, 1177, 1458
LmnI	GCTCC	2 cut(s)	415, 1240
Lsp1109I	GCAGC	4 cut(s)	419, 427, 1230, 1927
LweI	GCATC	4 cut(s)	1095, 1152, 1255, 1748
MaeI	CTAG	4 cut(s)	569, 1182, 1226, 1999
MaeII	ACGT	2 cut(s)	553, 1753
MaeIII	GTNAC	4 cut(s)	454, 886, 1414, 1451
MalI	GATC	4 cut(s)	270, 303, 1179, 1460
MboI	GATC	4 cut(s)	268, 301, 1177, 1458
MfeI	CAATTG	2 cut(s)	219, 1441
MhlI	GDGCHC	1 cut(s)	1282
MlyI	GAGTC	5 cut(s)	25, 194, 1207, 1462, 1850
MroI	TCCGGA	1 cut(s)	1249
MseI	TTAA	7 cut(s)	587, 855, 1041, 1173, 1431, 1521, 1782
MslI	CAYNNNNRTG	1 cut(s)	711
MspA1I	CMGCKG	1 cut(s)	1609
MspCI	CTTAAG	2 cut(s)	1430, 1781
MspI	CCGG	2 cut(s)	77, 1250
MspR9I	CCNGG	1 cut(s)	126
MunI	CAATTG	2 cut(s)	219, 1441
Mva1269I	GAATGC	3 cut(s)	379, 486, 1602
MvaI	CCWGG	1 cut(s)	126
MwoI	GCNNNNNNNGC	4 cut(s)	344, 374, 581, 811
NdeII	GATC	4 cut(s)	268, 301, 1177, 1458
NlaIII	CATG	9 cut(s)	308, 424, 491, 704, 716, 746, 814, 1034, 1844
NlaIV	GGNNCC	2 cut(s)	1086, 1905
NmuCI	GTSAC	2 cut(s)	886, 1414
NspI	RCATGY	2 cut(s)	814, 1844
PagI	TCATGA	1 cut(s)	304
PciI	ACATGT	1 cut(s)	1840
PcsI	WCGNNNNNNNCGW	1 cut(s)	328
PctI	GAATGC	3 cut(s)	379, 486, 1602
PfeI	GAWTC	4 cut(s)	176, 1381, 1437, 1513
PflFI	GACNNNGTC	1 cut(s)	184
PkrI	GCNGC	5 cut(s)	409, 442, 1245, 1882, 1942
PleI	GAGTC	5 cut(s)	25, 193, 1206, 1462, 1850
PmaCI	CACGTG	1 cut(s)	554
PmlI	CACGTG	1 cut(s)	554
PpsI	GAGTC	5 cut(s)	25, 193, 1206, 1462, 1850
Ppu21I	YACGTR	1 cut(s)	554
PpuMI	RGGWCCY	1 cut(s)	1085
PscI	ACATGT	1 cut(s)	1840
PshBI	ATTAAT	1 cut(s)	587
PsiI	TTATAA	2 cut(s)	654, 1235
Psp5II	RGGWCCY	1 cut(s)	1085
Psp6I	CCWGG	1 cut(s)	124
PspCI	CACGTG	1 cut(s)	554
PspGI	CCWGG	1 cut(s)	124
PspN4I	GGNNCC	2 cut(s)	1086, 1905
PspPI	GGNCC	3 cut(s)	643, 1085, 1422
PspPPI	RGGWCCY	1 cut(s)	1085
PstI	CTGCAG	3 cut(s)	409, 668, 1207
PsyI	GACNNNGTC	1 cut(s)	184
PvuII	CAGCTG	1 cut(s)	1609
RsaI	GTAC	5 cut(s)	519, 607, 1140, 1713, 1836
RsaNI	GTAC	5 cut(s)	518, 606, 1139, 1712, 1835
RseI	CAYNNNNRTG	1 cut(s)	711
SaqAI	TTAA	7 cut(s)	587, 855, 1041, 1173, 1431, 1521, 1782
SatI	GCNGC	5 cut(s)	408, 441, 1244, 1881, 1941
Sau3AI	GATC	4 cut(s)	268, 301, 1177, 1458
Sau96I	GGNCC	3 cut(s)	643, 1085, 1422
SbfI	CCTGCAGG	1 cut(s)	1207
ScaI	AGTACT	1 cut(s)	519
SchI	GAGTC	5 cut(s)	25, 194, 1207, 1462, 1850
ScrFI	CCNGG	1 cut(s)	126
SdaI	CCTGCAGG	1 cut(s)	1207
SduI	GDGCHC	1 cut(s)	1282
SfaNI	GCATC	4 cut(s)	1095, 1152, 1255, 1748
SfcI	CTRYAG	4 cut(s)	405, 618, 664, 1203
SinI	GGWCC	2 cut(s)	1085, 1422
SmiMI	CAYNNNNRTG	1 cut(s)	711
SmlI	CTYRAG	6 cut(s)	62, 348, 935, 1281, 1430, 1781
SmoI	CTYRAG	6 cut(s)	62, 348, 935, 1281, 1430, 1781
Sse8387I	CCTGCAGG	1 cut(s)	1207
SsiI	CCGC	1 cut(s)	1880
SspI	AATATT	1 cut(s)	1360
SspMI	CTAG	4 cut(s)	569, 1182, 1226, 1999
StyD4I	CCNGG	1 cut(s)	124
StyI	CCWWGG	1 cut(s)	382
TaaI	ACNGT	9 cut(s)	56, 148, 278, 294, 522, 791, 1020, 1598, 1992
TaiI	ACGT	2 cut(s)	556, 1756
TaqI	TCGA	8 cut(s)	174, 179, 271, 430, 596, 1366, 1959, 1987
TatI	WGTACW	4 cut(s)	517, 605, 1711, 1834
TauI	GCSGC	1 cut(s)	1883
TfiI	GAWTC	4 cut(s)	176, 1381, 1437, 1513
Tru1I	TTAA	7 cut(s)	587, 855, 1041, 1173, 1431, 1521, 1782
Tru9I	TTAA	7 cut(s)	587, 855, 1041, 1173, 1431, 1521, 1782
TscAI	CASTG	3 cut(s)	61, 1330, 1601
TseFI	GTSAC	2 cut(s)	886, 1414
TseI	GCWGC	4 cut(s)	407, 440, 1243, 1940
Tsp45I	GTSAC	2 cut(s)	886, 1414
TspGWI	ACGGA	3 cut(s)	197, 1211, 1678
TspRI	CASTG	3 cut(s)	61, 1330, 1601
Tth111I	GACNNNGTC	1 cut(s)	184
Vha464I	CTTAAG	2 cut(s)	1430, 1781
VpaK11BI	GGWCC	2 cut(s)	1085, 1422
VspI	ATTAAT	1 cut(s)	587
XapI	RAATTY	3 cut(s)	857, 1034, 1666
XceI	RCATGY	2 cut(s)	814, 1844
XspI	CTAG	4 cut(s)	569, 1182, 1226, 1999
ZrmI	AGTACT	1 cut(s)	519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.