MD03G1024000.v1.1

Protein slowmo homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011196
Physical Location & Seq
Forward (+)
1970958 .. 1973956
2999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1024000.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGTGAAGGCATACACGCAAGAGTACACATACAAGCACCCATGGGACCGGGTGACTTCCGCATCATGGAAAAAATTTGCTGATGCTGAAAACAAACGCACGCTATCACACATCCTTGAAGTTGACACTCTGAACCACAAGCTTGATCCCAGCACAGGAAAGCTTTACACCACACGTGCTATCACGGTCCATGCGCCAGGACCATGGTTTGTCCGCAAAATTGTTCGCCAGGATGTCTGCCACTGCGTCGAGTCAACAGTTGTGGATGCGCAAGCACGATCAATGCAACTCACCACCAATAATATTAGCCTTGAAAAGTTCATAGAGGTGGAGGAGAAGATCAGGTATGATCCCCACCCAGAGAACCCAAATGGCTGGACATTATGTCGACAGGAGACTAGCATTCGAATCAAGCCGTTATCAGCATTGGCATCGATGGCGGAAAAGGTTGAACAAAGATGCGCTGAGAAGTTTGTGCAGAACAGTGCCAAGGGCAGAGAGGTCATGGAGAGGATATGCAAGTATCTTGAAGCTGAATCTAAAGGGATTTCTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRELI

Protein Analysis

186

Amino Acids

21.3

Weight (kDa)

8.76

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRELI PF04707 17 - 178 1.1e-47 PRELI-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 270
AccI GTMKAC 1 cut(s) 388
AciI CCGC 3 cut(s) 60, 214, 440
AclWI GGATC 2 cut(s) 140, 344
AcsI RAATTY 1 cut(s) 74
AcvI CACGTG 1 cut(s) 176
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 66, 155
AflIII ACRYGT 1 cut(s) 173
AgsI TTSAA 4 cut(s) 119, 314, 452, 530
AhdI GACNNNNNGTC 1 cut(s) 384
AjnI CCWGG 2 cut(s) 196, 228
AluBI AGCT 3 cut(s) 142, 163, 533
AluI AGCT 3 cut(s) 142, 163, 533
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 2 cut(s) 140, 344
ApoI RAATTY 1 cut(s) 74
AspLEI GCGC 3 cut(s) 196, 271, 464
AspS9I GGNCC 3 cut(s) 46, 187, 200
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 3 cut(s) 16, 64, 283
AsuII TTCGAA 1 cut(s) 406
AvaII GGWCC 3 cut(s) 46, 187, 200
BbrPI CACGTG 1 cut(s) 176
BccI CCATC 1 cut(s) 430
BceAI ACGGC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 414, 448
BciT130I CCWGG 2 cut(s) 198, 230
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 1 cut(s) 399
Bme1390I CCNGG 3 cut(s) 50, 198, 230
Bme18I GGWCC 3 cut(s) 46, 187, 200
BmeRI GACNNNNNGTC 1 cut(s) 384
BmgT120I GGNCC 3 cut(s) 46, 187, 200
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 3 cut(s) 50, 198, 230
BmsI GCATC 5 cut(s) 71, 73, 256, 440, 449
Bpu14I TTCGAA 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 551
BsaJI CCNNGG 3 cut(s) 41, 203, 489
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 155
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 2 cut(s) 198, 230
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 3 cut(s) 41, 203, 489
BseGI GGATG 3 cut(s) 111, 238, 271
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 2 cut(s) 66, 155
BseMII CTCAG 1 cut(s) 456
BseRI GAGGAG 1 cut(s) 347
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 434
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 2 cut(s) 66, 155
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 406
Bsp143I GATC 4 cut(s) 145, 278, 339, 349
Bsp19I CCATGG 2 cut(s) 41, 203
BspACI CCGC 3 cut(s) 60, 214, 440
BspCNI CTCAG 1 cut(s) 457
BspDI ATCGAT 1 cut(s) 434
BspLI GGNNCC 1 cut(s) 47
BspPI GGATC 2 cut(s) 140, 344
BspT104I TTCGAA 1 cut(s) 406
BssECI CCNNGG 3 cut(s) 41, 203, 489
BssMI GATC 4 cut(s) 145, 278, 339, 349
BssT1I CCWWGG 3 cut(s) 41, 203, 489
Bst2UI CCWGG 2 cut(s) 198, 230
Bst4CI ACNGT 3 cut(s) 187, 259, 485
BstBAI YACGTR 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 2 cut(s) 101, 273
BstDEI CTNAG 1 cut(s) 465
BstDSI CCRYGG 2 cut(s) 41, 203
BstF5I GGATG 3 cut(s) 111, 238, 271
BstHHI GCGC 3 cut(s) 196, 271, 464
BstKTI GATC 4 cut(s) 148, 281, 342, 352
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 4 cut(s) 145, 278, 339, 349
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 2 cut(s) 198, 230
BstSCI CCNGG 3 cut(s) 48, 196, 228
BstXI CCANNNNNNTGG 2 cut(s) 204, 375
Bsu15I ATCGAT 1 cut(s) 434
BsuTUI ATCGAT 1 cut(s) 434
BtgI CCRYGG 2 cut(s) 41, 203
BtsCI GGATG 3 cut(s) 111, 238, 271
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 241, 490
Cac8I GCNNGC 2 cut(s) 101, 273
CfoI GCGC 3 cut(s) 196, 271, 464
Cfr13I GGNCC 3 cut(s) 46, 187, 200
ClaI ATCGAT 1 cut(s) 434
CseI GACGC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 25
CspCI CAANNNNNGTGG 2 cut(s) 243, 278
CviAII CATG 5 cut(s) 42, 66, 191, 204, 505
CviJI RGCY 6 cut(s) 142, 163, 309, 375, 415, 533
CviKI_1 RGCY 6 cut(s) 142, 163, 309, 375, 415, 533
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 465
DpnI GATC 4 cut(s) 147, 280, 341, 351
DpnII GATC 4 cut(s) 145, 278, 339, 349
DriI GACNNNNNGTC 1 cut(s) 384
Eam1105I GACNNNNNGTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 455
Eco130I CCWWGG 3 cut(s) 41, 203, 489
Eco47I GGWCC 3 cut(s) 46, 187, 200
Eco72I CACGTG 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 196, 228
EcoT14I CCWWGG 3 cut(s) 41, 203, 489
ErhI CCWWGG 3 cut(s) 41, 203, 489
FaeI CATG 5 cut(s) 45, 69, 194, 207, 508
FaqI GGGAC 1 cut(s) 59
FatI CATG 5 cut(s) 41, 65, 190, 203, 504
FblI GTMKAC 1 cut(s) 388
FokI GGATG 3 cut(s) 98, 245, 278
FspBI CTAG 1 cut(s) 399
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 3 cut(s) 195, 270, 463
GsaI CCCAGC 1 cut(s) 153
HapII CCGG 1 cut(s) 49
HgaI GACGC 1 cut(s) 235
HhaI GCGC 3 cut(s) 196, 271, 464
Hin1II CATG 5 cut(s) 45, 69, 194, 207, 508
Hin6I GCGC 3 cut(s) 194, 269, 462
HinP1I GCGC 3 cut(s) 194, 269, 462
HincII GTYRAC 3 cut(s) 124, 255, 389
HindII GTYRAC 3 cut(s) 124, 255, 389
HindIII AAGCTT 2 cut(s) 140, 161
HinfI GANTC 3 cut(s) 251, 408, 536
HpaII CCGG 1 cut(s) 49
HphI GGTGA 3 cut(s) 16, 64, 283
Hpy166II GTNNAC 4 cut(s) 27, 124, 255, 389
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 1 cut(s) 527
Hpy8I GTNNAC 4 cut(s) 27, 124, 255, 389
Hpy99I CGWCG 1 cut(s) 251
HpyCH4III ACNGT 3 cut(s) 187, 259, 485
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 3 cut(s) 286, 478, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 5 cut(s) 45, 69, 194, 207, 508
HspAI GCGC 3 cut(s) 194, 269, 462
Kzo9I GATC 4 cut(s) 145, 278, 339, 349
LweI GCATC 5 cut(s) 71, 73, 256, 440, 449
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 52
MalI GATC 4 cut(s) 147, 280, 341, 351
MboI GATC 4 cut(s) 145, 278, 339, 349
MboII GAAGA 1 cut(s) 349
MluCI AATT 2 cut(s) 74, 219
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 4 cut(s) 319, 325, 493, 504
MslI CAYNNNNRTG 1 cut(s) 326
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 3 cut(s) 50, 198, 230
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 2 cut(s) 198, 230
MwoI GCNNNNNNNGC 1 cut(s) 437
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 2 cut(s) 41, 203
NdeII GATC 4 cut(s) 145, 278, 339, 349
NlaIII CATG 5 cut(s) 45, 69, 194, 207, 508
NlaIV GGNNCC 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 52
NsbI TGCGCA 1 cut(s) 270
NspV TTCGAA 1 cut(s) 406
PctI GAATGC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 408, 536
PleI GAGTC 1 cut(s) 259
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 1 cut(s) 259
Ppu21I YACGTR 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 196, 228
PspCI CACGTG 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 2 cut(s) 196, 228
PspN4I GGNNCC 1 cut(s) 47
PspPI GGNCC 3 cut(s) 46, 187, 200
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 326
SalI GTCGAC 1 cut(s) 387
Sau3AI GATC 4 cut(s) 145, 278, 339, 349
Sau96I GGNCC 3 cut(s) 46, 187, 200
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 50, 198, 230
SetI ASST 8 cut(s) 144, 165, 178, 330, 347, 450, 504, 535
SfaNI GCATC 5 cut(s) 71, 73, 256, 440, 449
SfuI TTCGAA 1 cut(s) 406
SinI GGWCC 3 cut(s) 46, 187, 200
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 2 cut(s) 74, 219
SsiI CCGC 3 cut(s) 60, 214, 440
SspI AATATT 1 cut(s) 304
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 48, 196, 228
StyI CCWWGG 3 cut(s) 41, 203, 489
TaaI ACNGT 3 cut(s) 187, 259, 485
TaiI ACGT 1 cut(s) 178
TaqI TCGA 4 cut(s) 249, 388, 406, 434
TasI AATT 2 cut(s) 74, 219
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 2 cut(s) 408, 536
TscAI CASTG 2 cut(s) 248, 490
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 310
TspRI CASTG 2 cut(s) 248, 490
VpaK11BI GGWCC 3 cut(s) 46, 187, 200
XapI RAATTY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 388
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.