pycom11g02240

Protein slowmo homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011196
Physical Location & Seq
Forward (+)
1966235 .. 1966792
558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g02240.1

Sequence Viewer

Length: 558 bp
ATGGTGAAGGCATACGCACAAGAGTACTCATACAAGCACCCATGGGAGCGGGTGACTTCCGCATCATGGAAAAAATTTGCAGATGCTGAAAACAAACGCACACTATCACATATCCTTGAAGTTGACACTCTGAACCACAAGCTTGATCCCAGCACAGGAAAGCTTTACACCACACGTGCTATCACTGTCCATGCGCCAGGACCATGGTTTCTTCGCAAAATTGTTCGCCAGGATGTCTGCCACTGCGTTGAGTCAACAGTTGTGGATGCGCAAGCACGATCAATGCAACTCACCACCAATAATATTAGCCTCGAAAAGTTAATAGAGGTGGAGGAGAAGATCAGGATCGATCCCCACCCAGAAAACCCAAATGGTTGGACATTATGCCGACAGGAGACTAGCATTCGAATCAAGCCTTTATCAGCATTGGCATCAATGGCAGAAAAGGTTGAACAACGCTGCGCGGAGAAGTTTGTGCAGAACAGTGCCAAGGGTAGAGAGGTTATGGAGAAGATATGCAAGTATCTCGAAGCTGAATCTAAAGGGATTTCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRELI

Protein Analysis

186

Amino Acids

21.2

Weight (kDa)

8.76

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRELI PF04707 16 - 178 1.8e-45 PRELI-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 49
AccII CGCG 1 cut(s) 464
AciI CCGC 3 cut(s) 49, 60, 464
AclWI GGATC 3 cut(s) 140, 344, 353
AcsI RAATTY 1 cut(s) 74
AcvI CACGTG 1 cut(s) 176
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 66, 155
AflIII ACRYGT 1 cut(s) 173
AgsI TTSAA 2 cut(s) 119, 452
AjnI CCWGG 2 cut(s) 196, 228
AluBI AGCT 3 cut(s) 142, 163, 533
AluI AGCT 3 cut(s) 142, 163, 533
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 3 cut(s) 140, 344, 353
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 1 cut(s) 459
ApoI RAATTY 1 cut(s) 74
AspLEI GCGC 3 cut(s) 196, 271, 464
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 16, 64, 283
AsuII TTCGAA 1 cut(s) 406
AvaII GGWCC 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 176
BbvI GCAGC 1 cut(s) 446
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BciT130I CCWGG 2 cut(s) 198, 230
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 1 cut(s) 399
BisI GCNGC 1 cut(s) 460
BlsI GCNGC 1 cut(s) 461
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 198, 230
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 198, 230
BmsI GCATC 4 cut(s) 71, 73, 256, 440
Bpu14I TTCGAA 1 cut(s) 406
Bsa29I ATCGAT 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 41, 203, 489
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 155
Bse8I GATNNNNATC 1 cut(s) 344
BseBI CCWGG 2 cut(s) 198, 230
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 3 cut(s) 41, 203, 489
BseGI GGATG 2 cut(s) 238, 271
BseJI GATNNNNATC 1 cut(s) 344
BseLI CCNNNNNNNGG 2 cut(s) 66, 155
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 1 cut(s) 446
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 497
Bsh1236I CGCG 1 cut(s) 464
BshVI ATCGAT 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 66, 155
BsmAI GTCTC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 406
Bsp143I GATC 5 cut(s) 145, 278, 339, 345, 349
Bsp19I CCATGG 2 cut(s) 41, 203
BspACI CCGC 3 cut(s) 49, 60, 464
BspDI ATCGAT 1 cut(s) 348
BspFNI CGCG 1 cut(s) 464
BspPI GGATC 3 cut(s) 140, 344, 353
BspT104I TTCGAA 1 cut(s) 406
BsrBI CCGCTC 1 cut(s) 49
BssECI CCNNGG 3 cut(s) 41, 203, 489
BssMI GATC 5 cut(s) 145, 278, 339, 345, 349
BssT1I CCWWGG 3 cut(s) 41, 203, 489
Bst2UI CCWGG 2 cut(s) 198, 230
Bst4CI ACNGT 3 cut(s) 187, 259, 485
BstBAI YACGTR 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 1 cut(s) 273
BstDSI CCRYGG 2 cut(s) 41, 203
BstF5I GGATG 2 cut(s) 238, 271
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 3 cut(s) 196, 271, 464
BstKTI GATC 5 cut(s) 148, 281, 342, 348, 352
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 5 cut(s) 145, 278, 339, 345, 349
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 2 cut(s) 198, 230
BstSCI CCNGG 2 cut(s) 196, 228
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 446
BstXI CCANNNNNNTGG 2 cut(s) 204, 375
Bsu15I ATCGAT 1 cut(s) 348
BsuTUI ATCGAT 1 cut(s) 348
BtgI CCRYGG 2 cut(s) 41, 203
BtsCI GGATG 2 cut(s) 238, 271
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 3 cut(s) 183, 241, 490
Cac8I GCNNGC 1 cut(s) 273
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 3 cut(s) 196, 271, 464
Cfr13I GGNCC 1 cut(s) 200
ClaI ATCGAT 1 cut(s) 348
Csp6I GTAC 1 cut(s) 25
CspCI CAANNNNNGTGG 2 cut(s) 243, 278
CviAII CATG 4 cut(s) 42, 66, 191, 204
CviJI RGCY 5 cut(s) 142, 163, 309, 415, 533
CviKI_1 RGCY 5 cut(s) 142, 163, 309, 415, 533
CviQI GTAC 1 cut(s) 25
DpnI GATC 5 cut(s) 147, 280, 341, 347, 351
DpnII GATC 5 cut(s) 145, 278, 339, 345, 349
Eco130I CCWWGG 3 cut(s) 41, 203, 489
Eco47I GGWCC 1 cut(s) 200
Eco72I CACGTG 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 196, 228
EcoT14I CCWWGG 3 cut(s) 41, 203, 489
ErhI CCWWGG 3 cut(s) 41, 203, 489
FaeI CATG 4 cut(s) 45, 69, 194, 207
FatI CATG 4 cut(s) 41, 65, 190, 203
FauI CCCGC 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 460
FokI GGATG 2 cut(s) 245, 278
Fsp4HI GCNGC 1 cut(s) 460
FspBI CTAG 1 cut(s) 399
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 3 cut(s) 195, 270, 463
GluI GCNGC 1 cut(s) 460
GsaI CCCAGC 1 cut(s) 153
HhaI GCGC 3 cut(s) 196, 271, 464
Hin1II CATG 4 cut(s) 45, 69, 194, 207
Hin6I GCGC 3 cut(s) 194, 269, 462
HinP1I GCGC 3 cut(s) 194, 269, 462
HincII GTYRAC 2 cut(s) 124, 255
HindII GTYRAC 2 cut(s) 124, 255
HindIII AAGCTT 2 cut(s) 140, 161
HinfI GANTC 3 cut(s) 251, 408, 536
HphI GGTGA 3 cut(s) 16, 64, 283
Hpy166II GTNNAC 2 cut(s) 124, 255
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 2 cut(s) 343, 527
Hpy8I GTNNAC 2 cut(s) 124, 255
HpyCH4III ACNGT 3 cut(s) 187, 259, 485
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 80, 286, 478, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 4 cut(s) 45, 69, 194, 207
HspAI GCGC 3 cut(s) 194, 269, 462
Kzo9I GATC 5 cut(s) 145, 278, 339, 345, 349
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 9 cut(s) 141, 163, 183, 210, 215, 242, 328, 372, 377
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 4 cut(s) 71, 73, 256, 440
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 52
MalI GATC 5 cut(s) 147, 280, 341, 347, 351
MbiI CCGCTC 1 cut(s) 49
MboI GATC 5 cut(s) 145, 278, 339, 345, 349
MboII GAAGA 3 cut(s) 203, 349, 523
MluCI AATT 2 cut(s) 74, 219
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 4 cut(s) 319, 320, 325, 493
MseI TTAA 1 cut(s) 320
MspR9I CCNGG 2 cut(s) 198, 230
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 2 cut(s) 198, 230
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 437
NcoI CCATGG 2 cut(s) 41, 203
NdeII GATC 5 cut(s) 145, 278, 339, 345, 349
NlaIII CATG 4 cut(s) 45, 69, 194, 207
NmuCI GTSAC 1 cut(s) 52
NsbI TGCGCA 1 cut(s) 270
NspV TTCGAA 1 cut(s) 406
PctI GAATGC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 408, 536
PkrI GCNGC 1 cut(s) 461
PleI GAGTC 1 cut(s) 259
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 1 cut(s) 259
Ppu21I YACGTR 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 196, 228
PspCI CACGTG 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 2 cut(s) 196, 228
PspPI GGNCC 1 cut(s) 200
PstNI CAGNNNCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 1 cut(s) 460
Sau3AI GATC 5 cut(s) 145, 278, 339, 345, 349
Sau96I GGNCC 1 cut(s) 200
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 198, 230
SetI ASST 7 cut(s) 144, 165, 178, 330, 450, 504, 535
SfaNI GCATC 4 cut(s) 71, 73, 256, 440
SfuI TTCGAA 1 cut(s) 406
SinI GGWCC 1 cut(s) 200
Sse9I AATT 2 cut(s) 74, 219
SsiI CCGC 3 cut(s) 49, 60, 464
SspI AATATT 1 cut(s) 304
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 196, 228
StyI CCWWGG 3 cut(s) 41, 203, 489
TaaI ACNGT 3 cut(s) 187, 259, 485
TaiI ACGT 1 cut(s) 178
TaqI TCGA 4 cut(s) 312, 348, 406, 528
TasI AATT 2 cut(s) 74, 219
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 2 cut(s) 408, 536
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 3 cut(s) 190, 248, 490
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 459
Tsp45I GTSAC 1 cut(s) 52
TspRI CASTG 3 cut(s) 190, 248, 490
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 1 cut(s) 74
XspI CTAG 1 cut(s) 399
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.