Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007251

Physical Location & Seq

Genomic Coordinates Forward (+)

3792868 .. 3797545

Gene Structure 4678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1047100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2169 bp

ATGGAAGCGGTGGCGGAGGGGCTGTGGGCGCTGGCGGACCACCAGGAGCAGAAAGGGGAGATCGGGAGGGCGGTGAAGTGCCTGGAAGCCATATGCCAAAGCGACGTGTCGTTTTTCCCCATCGTCGAAGTCAAAACGCGCCTCCGTATCGCCACCCTGCTCCTGAAGCACTCGCACAACGTCAACCACGCCAAGTCGCACCTCGAGCGAGCTCAACTGCTCCTCAAGTCCATTCCTTCCTGCTTCGACCTCAAGTGCCGCGCCTACAGCCTCCTCAGCCAGTGCTACCACCTCGTCGGCGCCATTCCGCCGCAGAAGCAGATCCTCCACAAAGCCCTCGAGCTTTCCGCTTCCGCCGGCCACGAAATTACGGTCAAGCTATGGTCTTGCAACTTCAATTCTCAGCTTGCAAATGCTCTGATCATCGAAGGGGATTACCGGAGTTCTATCTCCGCCTTGGAGGCCGGTTTTGTTTGCGCTGCTGAAATATATTATCCCGAGTTACAGATGTTCTTTGCAACTTCTATCCTTCATGTACACCTAATGCAATGGGATGATGAGAGTACCGTTGAGCGAGCTGTTACCAAATGCAATGAAGTGTGGGAGTCCTTAGACCCGCAGAAAAGGCAACAATGCCTTGGTTTACTGTTCTATAATGAACTGCTGCACATATTTTACCGACTCCGGATATGTGACTACAAGAATGCTGGAGCACATGTAGAAAGATTGGATGCTGCTATGAAGGCCGATTTGCAGCAAATGCAGCATGTGCAGCAGCTGGGAAGGGAACTCGATGCTGTAAATCAGAGTCTTTCCCGGTTTGATCTACACCACAGGGAGAGGTCAGCATTGTCTGAAAAACAGGCTCGGCTTCAACACCAGCTCTCTAGTCTTTCAACATGGAGTTCAACTGCCCCGGGGTCCCTGGAACCAGCATATTTTGGAAATATGAAACGAACATATGGAGATAAGCTTGAGCTAGCACCGCCTCCTCTTGATGGGGAATGGCTACCAAAAAGTGCGGTTTATGCTCTTGTTGATCTTATGATGGTTGCGTTGAGTCGTCCAAAAGGAAATTTTAAGGACTCTGGAAAGCGTATACAATCCGGAATTCAAACTATCCAAGAGGAACTTGTGAAGCTTGGAATAACTGATGGTGTGAGAGAGGTGAATTTGCAACACTCTGCTATCTGGATGGCTGGTGTGTATTTGACGCTACTAATGCAGTTTCTTGAAAACAAAGTGGCTATGGAGTTGACACGATCTGAATTTGTAGAAGCACAAGAGGCATTGGTACAGATGAAAAATTGGTTTATGCGCTTCCCAACAATTTTACAGACATGTGAGAGCATCATCGAAATGCTCAGGGGGCAGTATGCTCATGCTGTTGGCTGTTATCATGAAGCAGCTTTCCATTACATTGAAGCTGCAAAGCTGACAGAGAGCAAATCAATGCAAGCTATCTGCCAAATTTATGCAGCTGTCTCCTACATCTGCATTGGTGATTCTGAATCATCTACACAGGCACTTGACTTGATTGGACCAGTTTACAGAATGATGGACTCATTTGTTGGAGTTCGAGAGAAAACCAGTGCTCTTTTCGCGTATGGTCTTTTATTGATGAAGCAACAAGATCTACAGGAAGCAAGAAATCAACTTGCCAAAGGTTTGCAGTTGACACACACTCATTTGGGGAACCTTCAGCTTGTTTCACAGTATTTGACAATCCTTGGGAGTTTGGCACTAGCACTTCATGATCCTGGACAGGCCAGAGAGATCTTGAGATCATCCTTAACATTGGCAAAGAAGCTTTCCGATATCCCAGCACAGATATCGGTGCTCTCTATTCTGACAGCTTTGTATCAAGAGTTAGGCGAAAAGGGACATGAATTGGAGAATCTTGAGTTCCAGAAGAAAAGGGCGGATGAACTGCAAAAGAGACTTGCAGATGCACATTCATCCATTTACCATATTGAACTAATTGAACAAGTGAAGGTTGAAGTACAACAATTTCACGGGGTTGACATCAACCGTGCAAACATGGGCCCATCCATAAGTGACAGTCTTGACATTCCAGAATCTATTGGTCTATCTGCCCAGTTGCCTACTCATTCGTCATCAAGGCTAGTTGATTTAGACTCCGGAAGACGTGGGAAGAGGAGAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000785 GO:0000819 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0032116 GO:0032501 GO:0032502 GO:0032991 GO:0034086 GO:0034088 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071840 GO:0097159 GO:0098813 GO:0140014 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cohesin_load

Protein Analysis

723

Amino Acids

81.16

Weight (kDa)

6.35

Isoelectric Point (pI)

46.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cohesin_load	PF10345	21 - 626	5.1e-31	Cohesin loading factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007251)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51340
fragaria_vesca	FvH4_3g41130 FvH4_3g41130 FvH4_3g41130 FvH4_3g41130 FvH4_3g41130
malus_domestica	MD03G1047100.v1.1
prunus_persica	Prupe.6G038300_v2.0.a1 Prupe.6G038300_v2.0.a1 Prupe.6G038300_v2.0.a1 Prupe.6G038300_v2.0.a1
pyrus_communis	pycom03g03710
rosa_chinensis	RchiOBHm_Chr5g0073921
rosa_laevigata	RLG00000036425
rosa_multiflora	Rmu_co8214482.1_g000001 Rmu_sc0000470.1_g000018 Rmu_sc0038496.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007450
rosa_rugosa	Rorug05G0427400 Rorug05G0427500
rosa_samantha	Rh5AG484400 Rh5BG505100 Rh5CG528900 Rh5DG517400
rosa_wichuraiana	Rw5G045030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	299
AccI	GTMKAC	1 cut(s)	1099
AccII	CGCG	3 cut(s)	139, 261, 1604
AccIII	TCCGGA	3 cut(s)	684, 1106, 2141
AclWI	GGATC	2 cut(s)	316, 1751
AcoI	YGGCCR	1 cut(s)	358
AcsI	RAATTY	6 cut(s)	1075, 1110, 1171, 1268, 1470, 2163
AcuI	CTGAAG	2 cut(s)	185, 1685
AcyI	GRCGYC	1 cut(s)	300
AfaI	GTAC	4 cut(s)	537, 565, 1296, 2004
AfiI	CCNNNNNNNGG	1 cut(s)	998
AflIII	ACRYGT	3 cut(s)	105, 715, 1340
AjiI	CACGTC	2 cut(s)	106, 2150
AjnI	CCWGG	4 cut(s)	42, 81, 924, 1759
Alw21I	GWGCWC	4 cut(s)	214, 715, 1597, 1842
Alw26I	GTCTC	2 cut(s)	1489, 1933
AlwI	GGATC	2 cut(s)	316, 1751
AlwNI	CAGNNNCTG	1 cut(s)	778
Ama87I	CYCGRG	4 cut(s)	203, 338, 497, 916
Aor13HI	TCCGGA	3 cut(s)	684, 1106, 2141
AoxI	GGCC	5 cut(s)	358, 462, 744, 1767, 2044
ApaI	GGGCCC	1 cut(s)	2048
ApoI	RAATTY	6 cut(s)	1075, 1110, 1171, 1268, 1470, 2163
ArsI	GACNNNNNNTTYG	2 cut(s)	357, 389
AspLEI	GCGC	6 cut(s)	31, 141, 263, 302, 479, 1320
AspS9I	GGNCC	5 cut(s)	37, 921, 1541, 2044, 2045
AsuC2I	CCSGG	3 cut(s)	817, 917, 918
AsuHPI	GGTGA	3 cut(s)	85, 1180, 1514
AsuNHI	GCTAGC	1 cut(s)	979
AvaI	CYCGRG	4 cut(s)	203, 338, 497, 916
AvaII	GGWCC	3 cut(s)	37, 921, 1541
BaeGI	GKGCMC	1 cut(s)	2048
BanI	GGYRCC	1 cut(s)	299
BanII	GRGCYC	2 cut(s)	214, 2048
BbsI	GAAGAC	1 cut(s)	2152
Bbv12I	GWGCWC	4 cut(s)	214, 715, 1597, 1842
BbvCI	CCTCAGC	1 cut(s)	275
BccI	CCATC	7 cut(s)	128, 992, 1042, 1148, 1189, 1552, 2056
BcgI	CGANNNNNNTGC	2 cut(s)	1815, 1849
BciT130I	CCWGG	4 cut(s)	44, 83, 926, 1761
BclI	TGATCA	1 cut(s)	420
BcnI	CCSGG	3 cut(s)	817, 917, 918
BcoDI	GTCTC	2 cut(s)	1489, 1933
BfaI	CTAG	4 cut(s)	888, 980, 1745, 2126
BfmI	CTRYAG	2 cut(s)	265, 1637
BfoI	RGCGCY	2 cut(s)	32, 303
BglI	GCCNNNNNGGC	1 cut(s)	461
BglII	AGATCT	2 cut(s)	1633, 1776
Bme1390I	CCNGG	7 cut(s)	44, 83, 817, 917, 918, 926, 1761
Bme18I	GGWCC	3 cut(s)	37, 921, 1541
BmeT110I	CYCGRG	4 cut(s)	203, 338, 497, 916
BmgBI	CACGTC	2 cut(s)	106, 2150
BmgT120I	GGNCC	5 cut(s)	37, 921, 1541, 2044, 2045
BmiI	GGNNCC	6 cut(s)	301, 922, 923, 930, 1697, 2046
BmrFI	CCNGG	7 cut(s)	44, 83, 817, 917, 918, 926, 1761
BmrI	ACTGGG	1 cut(s)	2092
BmsI	GCATC	4 cut(s)	721, 784, 1359, 1939
BmtI	GCTAGC	1 cut(s)	983
BmuI	ACTGGG	1 cut(s)	2092
BpiI	GAAGAC	1 cut(s)	2152
BpmI	CTGGAG	1 cut(s)	729
Bpu10I	CCTNAGC	2 cut(s)	275, 1364
BpuEI	CTTGAG	5 cut(s)	209, 236, 995, 1801, 1922
BpuMI	CCSGG	3 cut(s)	817, 917, 918
BsaHI	GRCGYC	1 cut(s)	300
BsaJI	CCNNGG	7 cut(s)	456, 637, 915, 916, 917, 924, 1729
BsaWI	WCCGGW	4 cut(s)	438, 684, 1106, 2141
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 702, 732
Bsc4I	CCNNNNNNNGG	1 cut(s)	998
Bse118I	RCCGGY	2 cut(s)	356, 464
Bse1I	ACTGG	4 cut(s)	280, 1544, 1590, 2098
Bse3DI	GCAATG	2 cut(s)	554, 598
BseAI	TCCGGA	3 cut(s)	684, 1106, 2141
BseBI	CCWGG	4 cut(s)	44, 83, 926, 1761
BseDI	CCNNGG	7 cut(s)	456, 637, 915, 916, 917, 924, 1729
BseGI	GGATG	7 cut(s)	559, 736, 1200, 1787, 1930, 1958, 2048
BseLI	CCNNNNNNNGG	1 cut(s)	998
BseMI	GCAATG	2 cut(s)	554, 598
BseMII	CTCAG	3 cut(s)	289, 416, 1378
BseNI	ACTGG	4 cut(s)	280, 1544, 1590, 2098
BseRI	GAGGAG	3 cut(s)	212, 263, 981
BseSI	GKGCMC	1 cut(s)	2048
BseYI	CCCAGC	2 cut(s)	778, 1822
BsgI	GTGCAG	2 cut(s)	650, 791
Bsh1236I	CGCG	3 cut(s)	139, 261, 1604
BshFI	GGCC	5 cut(s)	360, 464, 746, 1769, 2046
BshNI	GGYRCC	1 cut(s)	299
BsiHKAI	GWGCWC	4 cut(s)	214, 715, 1597, 1842
BsiHKCI	CYCGRG	4 cut(s)	203, 338, 497, 916
BsiSI	CCGG	8 cut(s)	357, 439, 465, 685, 817, 917, 1107, 2142
BslFI	GGGAC	2 cut(s)	907, 1896
BslI	CCNNNNNNNGG	1 cut(s)	998
BsmAI	GTCTC	2 cut(s)	1489, 1933
BsmFI	GGGAC	2 cut(s)	907, 1896
BsmI	GAATGC	1 cut(s)	709
BsnI	GGCC	5 cut(s)	360, 464, 746, 1769, 2046
BsoBI	CYCGRG	4 cut(s)	203, 338, 497, 916
Bsp120I	GGGCCC	1 cut(s)	2044
Bsp1286I	GDGCHC	5 cut(s)	214, 715, 1597, 1842, 2048
Bsp13I	TCCGGA	3 cut(s)	684, 1106, 2141
Bsp1407I	TGTACA	1 cut(s)	535
BspANI	GGCC	5 cut(s)	360, 464, 746, 1769, 2046
BspCNI	CTCAG	3 cut(s)	288, 415, 1377
BspEI	TCCGGA	3 cut(s)	684, 1106, 2141
BspFNI	CGCG	3 cut(s)	139, 261, 1604
BspHI	TCATGA	2 cut(s)	1399, 1753
BspLI	GGNNCC	6 cut(s)	301, 922, 923, 930, 1697, 2046
BspOI	GCTAGC	1 cut(s)	983
BspPI	GGATC	2 cut(s)	316, 1751
BspT107I	GGYRCC	1 cut(s)	299
BsrDI	GCAATG	2 cut(s)	554, 598
BsrFI	RCCGGY	2 cut(s)	356, 464
BsrGI	TGTACA	1 cut(s)	535
BsrI	ACTGG	4 cut(s)	280, 1544, 1590, 2098
BssAI	RCCGGY	2 cut(s)	356, 464
BssECI	CCNNGG	7 cut(s)	456, 637, 915, 916, 917, 924, 1729
BssNAI	GTATAC	1 cut(s)	1100
BssNI	GRCGYC	1 cut(s)	300
BssT1I	CCWWGG	3 cut(s)	456, 637, 1729
Bst1107I	GTATAC	1 cut(s)	1100
Bst2UI	CCWGG	4 cut(s)	44, 83, 926, 1761
Bst4CI	ACNGT	6 cut(s)	373, 568, 648, 1716, 2033, 2063
Bst6I	CTCTTC	1 cut(s)	2150
BstACI	GRCGYC	1 cut(s)	300
BstAPI	GCANNNNNTGC	2 cut(s)	760, 769
BstAUI	TGTACA	1 cut(s)	535
BstC8I	GCNNGC	7 cut(s)	33, 210, 358, 408, 576, 981, 1458
BstDEI	CTNAG	4 cut(s)	275, 402, 610, 1364
BstF5I	GGATG	7 cut(s)	559, 736, 1200, 1787, 1930, 1958, 2048
BstFNI	CGCG	3 cut(s)	139, 261, 1604
BstH2I	RGCGCY	2 cut(s)	32, 303
BstHHI	GCGC	6 cut(s)	31, 141, 263, 302, 479, 1320
BstMAI	GTCTC	2 cut(s)	1489, 1933
BstNI	CCWGG	4 cut(s)	44, 83, 926, 1761
BstNSI	RCATGY	3 cut(s)	719, 770, 1344
BstSCI	CCNGG	7 cut(s)	42, 81, 815, 915, 916, 924, 1759
BstSFI	CTRYAG	2 cut(s)	265, 1637
BstSLI	GKGCMC	1 cut(s)	2048
BstUI	CGCG	3 cut(s)	139, 261, 1604
BstV2I	GAAGAC	1 cut(s)	2152
BstX2I	RGATCY	3 cut(s)	321, 1633, 1776
BstYI	RGATCY	3 cut(s)	321, 1633, 1776
BstZ17I	GTATAC	1 cut(s)	1100
BsuRI	GGCC	5 cut(s)	360, 464, 746, 1769, 2046
BtrI	CACGTC	2 cut(s)	106, 2150
BtsCI	GGATG	7 cut(s)	559, 736, 1200, 1787, 1930, 1958, 2048
BtsIMutI	CAGTG	2 cut(s)	287, 1597
Cac8I	GCNNGC	7 cut(s)	33, 210, 358, 408, 576, 981, 1458
CaiI	CAGNNNCTG	1 cut(s)	778
CciI	TCATGA	2 cut(s)	1399, 1753
CfoI	GCGC	6 cut(s)	31, 141, 263, 302, 479, 1320
Cfr10I	RCCGGY	2 cut(s)	356, 464
Cfr13I	GGNCC	5 cut(s)	37, 921, 1541, 2044, 2045
Cfr9I	CCCGGG	1 cut(s)	916
CseI	GACGC	1 cut(s)	1222
Csp6I	GTAC	4 cut(s)	536, 564, 1295, 2003
CviQI	GTAC	4 cut(s)	536, 564, 1295, 2003
DdeI	CTNAG	4 cut(s)	275, 402, 610, 1364
DinI	GGCGCC	1 cut(s)	301
EaeI	YGGCCR	1 cut(s)	358
Eam1104I	CTCTTC	1 cut(s)	2150
EarI	CTCTTC	1 cut(s)	2150
EciI	GGCGGA	6 cut(s)	29, 50, 297, 343, 442, 1937
Ecl136II	GAGCTC	1 cut(s)	212
Eco130I	CCWWGG	3 cut(s)	456, 637, 1729
Eco24I	GRGCYC	2 cut(s)	214, 2048
Eco32I	GATATC	2 cut(s)	1819, 1833
Eco47I	GGWCC	3 cut(s)	37, 921, 1541
Eco53kI	GAGCTC	1 cut(s)	212
Eco57I	CTGAAG	2 cut(s)	185, 1685
Eco88I	CYCGRG	4 cut(s)	203, 338, 497, 916
EcoICRI	GAGCTC	1 cut(s)	212
EcoO109I	RGGNCCY	1 cut(s)	921
EcoRI	GAATTC	1 cut(s)	1110
EcoRII	CCWGG	4 cut(s)	42, 81, 924, 1759
EcoRV	GATATC	2 cut(s)	1819, 1833
EcoT14I	CCWWGG	3 cut(s)	456, 637, 1729
EcoT38I	GRGCYC	2 cut(s)	214, 2048
EgeI	GGCGCC	1 cut(s)	301
EheI	GGCGCC	1 cut(s)	301
ErhI	CCWWGG	3 cut(s)	456, 637, 1729
FalI	AAGNNNNNCTT	2 cut(s)	1116, 1148
FaqI	GGGAC	2 cut(s)	907, 1896
FauI	CCCGC	1 cut(s)	624
FauNDI	CATATG	2 cut(s)	92, 961
FbaI	TGATCA	1 cut(s)	420
FblI	GTMKAC	1 cut(s)	1099
FokI	GGATG	7 cut(s)	566, 743, 1207, 1774, 1937, 1945, 2035
FriOI	GRGCYC	2 cut(s)	214, 2048
FspBI	CTAG	4 cut(s)	888, 980, 1745, 2126
GlaI	GCGC	6 cut(s)	30, 140, 262, 301, 478, 1319
GsaI	CCCAGC	2 cut(s)	782, 1826
GsuI	CTGGAG	1 cut(s)	729
HaeII	RGCGCY	2 cut(s)	32, 303
HaeIII	GGCC	5 cut(s)	360, 464, 746, 1769, 2046
HapII	CCGG	8 cut(s)	357, 439, 465, 685, 817, 917, 1107, 2142
HgaI	GACGC	1 cut(s)	1222
HhaI	GCGC	6 cut(s)	31, 141, 263, 302, 479, 1320
Hin1I	GRCGYC	1 cut(s)	300
Hin6I	GCGC	6 cut(s)	29, 139, 261, 300, 477, 1318
HinP1I	GCGC	6 cut(s)	29, 139, 261, 300, 477, 1318
HincII	GTYRAC	4 cut(s)	184, 1257, 1677, 2023
HindII	GTYRAC	4 cut(s)	184, 1257, 1677, 2023
HindIII	AAGCTT	3 cut(s)	971, 1139, 1808
HpaII	CCGG	8 cut(s)	357, 439, 465, 685, 817, 917, 1107, 2142
HphI	GGTGA	3 cut(s)	85, 1180, 1514
Hpy166II	GTNNAC	8 cut(s)	184, 538, 644, 1100, 1257, 1549, 1677, 2023
Hpy188I	TCNGA	7 cut(s)	420, 807, 856, 1267, 1510, 1816, 1851
Hpy8I	GTNNAC	8 cut(s)	184, 538, 644, 1100, 1257, 1549, 1677, 2023
Hpy99I	CGWCG	3 cut(s)	107, 128, 299
HpyAV	CCTTC	7 cut(s)	246, 422, 539, 736, 777, 1709, 1987
HpyCH4III	ACNGT	6 cut(s)	373, 568, 648, 1716, 2033, 2063
HpyCH4IV	ACGT	3 cut(s)	105, 180, 2149
HpyF3I	CTNAG	4 cut(s)	275, 402, 610, 1364
HpySE526I	ACGT	3 cut(s)	105, 180, 2149
Hsp92I	GRCGYC	1 cut(s)	300
HspAI	GCGC	6 cut(s)	29, 139, 261, 300, 477, 1318
KasI	GGCGCC	1 cut(s)	299
KflI	GGGWCCC	1 cut(s)	921
Kpn2I	TCCGGA	3 cut(s)	684, 1106, 2141
KroI	GCCGGC	1 cut(s)	356
KroNI	GCCGGC	1 cut(s)	358
Ksp22I	TGATCA	1 cut(s)	420
LmnI	GCTCC	4 cut(s)	46, 165, 225, 710
LweI	GCATC	4 cut(s)	721, 784, 1359, 1939
MaeI	CTAG	4 cut(s)	888, 980, 1745, 2126
MaeII	ACGT	3 cut(s)	105, 180, 2149
MaeIII	GTNAC	4 cut(s)	501, 580, 692, 2057
MboII	GAAGA	3 cut(s)	1924, 2157, 2167
MflI	RGATCY	3 cut(s)	321, 1633, 1776
MhlI	GDGCHC	5 cut(s)	214, 715, 1597, 1842, 2048
Mly113I	GGCGCC	1 cut(s)	300
MlyI	GAGTC	7 cut(s)	614, 675, 817, 1069, 1079, 1556, 2132
MmeI	TCCRAC	1 cut(s)	1552
MroI	TCCGGA	3 cut(s)	684, 1106, 2141
MroNI	GCCGGC	1 cut(s)	356
MseI	TTAA	2 cut(s)	1080, 1793
MslI	CAYNNNNRTG	1 cut(s)	1358
MspA1I	CMGCKG	2 cut(s)	778, 1481
MspI	CCGG	8 cut(s)	357, 439, 465, 685, 817, 917, 1107, 2142
MspR9I	CCNGG	7 cut(s)	44, 83, 817, 917, 918, 926, 1761
Mva1269I	GAATGC	1 cut(s)	709
MvaI	CCWGG	4 cut(s)	44, 83, 926, 1761
MvnI	CGCG	3 cut(s)	139, 261, 1604
NaeI	GCCGGC	1 cut(s)	358
NarI	GGCGCC	1 cut(s)	300
NciI	CCSGG	3 cut(s)	817, 917, 918
NdeI	CATATG	2 cut(s)	92, 961
NgoMIV	GCCGGC	1 cut(s)	356
NheI	GCTAGC	1 cut(s)	979
NlaIV	GGNNCC	6 cut(s)	301, 922, 923, 930, 1697, 2046
NmeAIII	GCCGAG	1 cut(s)	847
NmuCI	GTSAC	2 cut(s)	692, 2057
NspI	RCATGY	3 cut(s)	719, 770, 1344
PaeR7I	CTCGAG	2 cut(s)	203, 338
PagI	TCATGA	2 cut(s)	1399, 1753
PciI	ACATGT	2 cut(s)	715, 1340
PctI	GAATGC	1 cut(s)	709
PdiI	GCCGGC	1 cut(s)	358
PfeI	GAWTC	4 cut(s)	1505, 1511, 1897, 2078
PfoI	TCCNGGA	1 cut(s)	1759
PleI	GAGTC	7 cut(s)	613, 675, 816, 1068, 1079, 1556, 2132
PluTI	GGCGCC	1 cut(s)	303
PpsI	GAGTC	7 cut(s)	613, 675, 816, 1068, 1079, 1556, 2132
PpuMI	RGGWCCY	1 cut(s)	921
PscI	ACATGT	2 cut(s)	715, 1340
Psp124BI	GAGCTC	1 cut(s)	214
Psp5II	RGGWCCY	1 cut(s)	921
Psp6I	CCWGG	4 cut(s)	42, 81, 924, 1759
PspFI	CCCAGC	2 cut(s)	778, 1822
PspGI	CCWGG	4 cut(s)	42, 81, 924, 1759
PspN4I	GGNNCC	6 cut(s)	301, 922, 923, 930, 1697, 2046
PspOMI	GGGCCC	1 cut(s)	2044
PspPI	GGNCC	5 cut(s)	37, 921, 1541, 2044, 2045
PspPPI	RGGWCCY	1 cut(s)	921
PspXI	VCTCGAGB	2 cut(s)	203, 338
PstNI	CAGNNNCTG	1 cut(s)	778
PsuI	RGATCY	3 cut(s)	321, 1633, 1776
PvuII	CAGCTG	2 cut(s)	778, 1481
RsaI	GTAC	4 cut(s)	537, 565, 1296, 2004
RsaNI	GTAC	4 cut(s)	536, 564, 1295, 2003
RseI	CAYNNNNRTG	1 cut(s)	1358
SacI	GAGCTC	1 cut(s)	214
SaqAI	TTAA	2 cut(s)	1080, 1793
Sau96I	GGNCC	5 cut(s)	37, 921, 1541, 2044, 2045
SchI	GAGTC	7 cut(s)	614, 675, 817, 1069, 1079, 1556, 2132
ScrFI	CCNGG	7 cut(s)	44, 83, 817, 917, 918, 926, 1761
SduI	GDGCHC	5 cut(s)	214, 715, 1597, 1842, 2048
SfaNI	GCATC	4 cut(s)	721, 784, 1359, 1939
SfcI	CTRYAG	2 cut(s)	265, 1637
SfoI	GGCGCC	1 cut(s)	301
Sfr274I	CTCGAG	2 cut(s)	203, 338
SinI	GGWCC	3 cut(s)	37, 921, 1541
SlaI	CTCGAG	2 cut(s)	203, 338
SmaI	CCCGGG	1 cut(s)	918
SmiMI	CAYNNNNRTG	1 cut(s)	1358
SmlI	CTYRAG	7 cut(s)	203, 224, 251, 338, 974, 1780, 1901
SmoI	CTYRAG	7 cut(s)	203, 224, 251, 338, 974, 1780, 1901
SspDI	GGCGCC	1 cut(s)	299
SspMI	CTAG	4 cut(s)	888, 980, 1745, 2126
SstI	GAGCTC	1 cut(s)	214
StyD4I	CCNGG	7 cut(s)	42, 81, 815, 915, 916, 924, 1759
StyI	CCWWGG	3 cut(s)	456, 637, 1729
TaaI	ACNGT	6 cut(s)	373, 568, 648, 1716, 2033, 2063
TaiI	ACGT	3 cut(s)	108, 183, 2152
TaqI	TCGA	8 cut(s)	126, 204, 246, 339, 426, 792, 1356, 1579
TatI	WGTACW	2 cut(s)	535, 2002
TauI	GCSGC	2 cut(s)	261, 313
TfiI	GAWTC	4 cut(s)	1505, 1511, 1897, 2078
Tru1I	TTAA	2 cut(s)	1080, 1793
Tru9I	TTAA	2 cut(s)	1080, 1793
TscAI	CASTG	2 cut(s)	287, 1597
TseFI	GTSAC	2 cut(s)	692, 2057
Tsp45I	GTSAC	2 cut(s)	692, 2057
TspGWI	ACGGA	1 cut(s)	134
TspMI	CCCGGG	1 cut(s)	916
TspRI	CASTG	2 cut(s)	287, 1597
VpaK11BI	GGWCC	3 cut(s)	37, 921, 1541
XapI	RAATTY	6 cut(s)	1075, 1110, 1171, 1268, 1470, 2163
XceI	RCATGY	3 cut(s)	719, 770, 1344
XhoI	CTCGAG	2 cut(s)	203, 338
XmaI	CCCGGG	1 cut(s)	916
XmiI	GTMKAC	1 cut(s)	1099
XspI	CTAG	4 cut(s)	888, 980, 1745, 2126

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.