Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0007251

Physical Location & Seq

Genomic Coordinates Forward (+)

59440735 .. 59445764

Gene Structure 5030 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0427500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4353 bp

ATGGGGACTGAATTACCCTCCTCCTCATTTGCCTCTTCTTCAAACACACAATGGACATATGACGTTTTTCTCAGTTTCCGAGGTGAGGATACTCGTAACAGCTTTACAGATCATCTATATGCTGCTCTAGACCGAGAAGGTATTTTCACCTTTAGGGATGATGAAGAGCTTGATAGAGGACAACCCATTTCATCAAATCTCCTGGAAGCCATACAAGTTTCAAGAATCGCGCTTGTTGTTATCTCGAGAAACTATGCTTGGTCAACCTGGTGTTTGGATGAACTCACTATGATTGTTGAGTGCATGGAGGGCAGGGGGCAGAGAGTGTTTCCAATTTTCTATGATGTAGATCCAGGCGAGGTAAGGAAACAGACCGGATGGTTTGGACAAGCCTTTGTTGCACATGAAGAGCGCTTTAAGGATGACTTGGAGAAAGACAAGGTGCAAAAGTGGAGAGCTGCTTTGACTCGAGTGGCCAATCTATCTGGATTTCATTTACGCGATAGGTATGAGTCAAAGTTTATCCAAGAGATTATGGGAAAGATAGTAAATGAGTTGAGCGGTACAATCCAACAGCCTCATTCGGATGATCTTGGACTTGTGGGAATTAACTTTGGTGTTGAGCAAATAATGAAGTTATGTTCAGCTACAGATCAAGGGAATGATGTTGTCTTTGTAGGTATATGGGGAATGGGCGGGGTTGGCAAGACGACTCTTGCACAAGCTTTTTATGATAAAATCGCTAATCAATTTCGAGATAAATGGTTTCTGAAAAATGTTAGAGAGATTTCTAGACAAGACGGTATTGTTGCCTTAACAAAAGATCTCCTTTGCAATGTCTTAAAAAGGATGGATGTGTATGATAGTGAGATAAGGCATAGATTTCGTCGTAAAAAGGTTCTCATCATTCTGGATGATGTAGATAAGTTGGACCAATTGCAAGCATTAGCTGGAAGCAAAGATTGGTTTGGTCGAGGGAGTAGGATCATTATAACAACCAGGGACGAAAAATTGTTGAGTGCTCATCGTGTGGATAGATGTCACAAGGTCAAGGAATTGAAAAGTGATGATGGTCTTAAGCTTTTCAGTTGGAAAGCCTTCCAGAATGATCAACCCCCAAAAGATTTTATAGAGTTATCCCATAACTTTGTAAATTATAGTAAGGGCCTTCCTTTAGCTATTGAAGTTTTGGGTTCTTTCTTGTGTGGTCAAAGCCATCAAGAATGGGAGAGTGCCTTTAGTAGAATACAAGAAAATCTTGACAAAGATATCATGTCTGCACTCAGAATCAGTTTTGATGGATTGCATGAGAAGGAGAAGCAAATATTTTTAGATATTGCATGTTTCTTCAAAGGACAGGACAGGGATGAGGTGACAATATTGTTGGAGGGTTTTGGTTTTGACCCAATCATTGGTATAAGTGCTCTCATTCGAAAATCCCTCATCACTATAGTAGGGAGTAAATTGTGGATGCATGATTTGCTTCAGGAAATGGGTTGGGAACTTGTTCATGGGGAATGTCACAATGAACCTGGGAATCGTAGTAGATTATGGCAACCGGCTGACGTGCTTGATTTACTGAAGTATCATAAGGGATCAGATGCAGTTCAAGGGATAGTCCTTAGCTTGCCTCAAGAACAGAAGAAAGAATTGAAATGTGACGCTCTGTCAAACATGAAAAAATTGAGATTTCTTAAAATATCTAATGTGCTCCTTCCTCTTGGCCTCGATTATCTATCTACTGAGTTAAGAGCTCTCGAATGGCATGGATATCCTTCAAAGTTGTTGCCAAGAGCTTTCCCATCTGAGAAGCTTGTTCACCTCAACCTGTGTGGCAGCCTTATCAAACATCTATGGAAAGGATTCAAGTGTTTAGATAAGTTAAAATCAATTGATTTGAAGGACTCTGAAAATTTGATTGAGACACCAGACTTCACGGGTGTCCCAAATCTTGAGAGGCTGAATCTTGAAGGTTGCACAAAATTATCTAAGGTTCATCCATCCATTAGCAATCTCAGACGCCTTAGATTATTGAATATGAAAAACTGTAAAAGCCTTCAGATCACTTCATGGGATATCAGTTTGGAATCTCTCGAAATTTTCAATCTTTCGGGCTGTGCACGACTCAAGAGATTTCCAGAGATTGTGGGAGATATGAAATGCTTGTCTAAAATTTATTTAGATGGGAGTGCTATAGAGGAATTACCAGTGTCAATTGAGCGTCTGACTGGTCTTACATTGTTGGATATGGCTCATTGCAAAAATATTTTGAGAATTCCTAGTGTCATTTCAAGTTTGACAGGGCTTAAAACAATCATTCTGTCAGGCTGCTCTAATCTTATTGAAATTCCTGAGAACATTGGGAATGTGGAATGTCTTGAAGAGTTGGATATAAGTGGAACTGCTATAAGGCAAATGCCATCATCCGTTGTGTATCGCAAAAACCTCAAAGAGTTATTCCGCTCCAGGTGCAGTGGTCAAGTTTCTGCATCACTGCTCGTTCCCTGTCTTCCACTTCGCCGTGGAAAGGGCTTCTTGTTACCTACTTCATTCTCTGGTTTTAGCTCTTTAATGTCATTGAATTTAAGTTACTGCAATCTGTCTGATGGAGCTCTCCGTAATTTTTTGGACTGCATGTCATCATTGGTGGAGTTGAATTTACAGGGAAACAATTTTACAAGCATACCTGAAAGTATTTGCGGACTTTCTAAGCTTGTAATGCTTAACTTGGATGATTGTAGCCGATTACAGTCCTTGCCAAAGCTTCCATCGAATATAGAAAATGTTGGGGCAATTGGTTGTATGTCACTTGAAAGTGATTCTGATCAGTATGAAGTATTTCGTTCAATAAACAAAAACCGTGTTGCGATTAATAAGCGCACAACATCAACCGTATGTTCCGTTCGGATGTCCAATTCACAATTTGCAGCATGGCTGAACAGATATCATGAGGTTAATGAGGTACAGGTTGAGATAAGTTTACCGGTGAGAACTGCATATGGTTTATCAATTGGACCTCGTGGAAATATTCCAAAGTGGTTCAACAATAGAAGCAGCAACTTTTCTATAACAATCCCGCTTTCAAAGGACTTAACTAATAAGAGTAGGCTATGGATGGGATGTGCTTTCTATGTTGGTTTGGAAGTTGATGAGAATTTTGATACCAGTTCTGATTTCAAAGTCAGGCACAAGTTTGACTGTTGTTTTGATACCATGGAAGGTCCTGGTTACAAACAAACTCTATACCACGAGGTAAATGCTAAAGGTGTCCATTGGACTGGGTCAATTGGATATTGGATAATAATACCGCATCTGTGGTTTTCAGCAAGGTTGAATGCCTTCAATCAGTGCGGTAGGATCGAGGCTAACACTACAATTGATAGCCCATGTGTGGAGGTTAAAATGAGTGGTGCCCGTCTACTCTACGAAGGAACAGATTTTATAGAGTTTATCCAAGCAATAACAAACTCGACTTGGGCTTCAAGAATTAGGCAGAATAACAGTTCCTCTGTAAGTCTGTTGGCTGAGGATAGTAGCTGCAAAATTGACTCGAGCATTTTGTTGAAAGAACATCTTAGGTCGCTGCCTTCAGATTTAAAATGCTCTGAGGGGCAATGCGAGGAGGGATTCAAGTACTACGATATTTACGGCAAAGTCGTTCATCCCCAGACCTCGTCTCTGTTAGGAGAAATCCTTCAGATATATTCTGGTCAGCACCGCGTGTATTATGGAGAAACTTTTCCTCAATATAGAATTCCAGAATGGTTCTGCAGTGTGAATGGTTCCTTGGCAGCCTTCCCGCTAAATCCTATGAATGAGTGGAAAGGAATAGCTGCATGTGCCGTTTTCACAGTCCTTGACAGTGTGGATCCCGAAAAGACTGGCATATGCTGTTATTTGGAATCTGGCTATCGGAGTATCACAATAGAGGGACGCATCACTGAATCTCTTATTAGTTTGAAGGTTCGTGGTTTCGTCAGTATGTTTTATGTATCACGTGGGTCACTTTCAGATGTTATGTCATCTGATTGCACTTCCATCATCAAAGTCGATTTTTATAGTAAGAGCAAGAAGATAACGGTGCAGAGTTGTGGGTATCAAATTGTATTCCATGAAAATATGGAAGAATTTATGGAAACAATACTGCAATGTTCAATCGCATCTCAGACTTCTCAGTTGTCAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAGGGGAACTCTCTCTACCCAAATAGAAAAGGAGAAAAGATACAAGTAGAGAAGAAACACCGAAAGAAAAGGAGAACTCTCTCTACCCGAATTTTGAAGTCTCCCTGTAGATCGATCTGTTGCTTCCTGTGTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000278 GO:0000280 GO:0000785 GO:0000819 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016043 GO:0022402 GO:0022414 GO:0032116 GO:0032501 GO:0032502 GO:0032991 GO:0034086 GO:0034088 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0071840 GO:0097159 GO:0098813 GO:0140014 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cohesin_load

Protein Analysis

1450

Amino Acids

164.19

Weight (kDa)

7.7

Isoelectric Point (pI)

42.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	20 - 196	2.7e-53	TIR domain
TIR_2	PF13676	22 - 119	3.5e-14	TIR domain
NB-ARC	PF00931	218 - 370	1.1e-19	NB-ARC domain
NACHT	PF05729	228 - 357	9e-08	NACHT domain
WHD_ROQ1	PF23282	437 - 504	2.6e-17	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	664 - 772	7.8e-09	Leucine-rich repeat region
LRR_13	PF23286	695 - 782	3.1e-12	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13	PF23286	781 - 944	1.8e-07	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8	PF13855	856 - 913	4.3e-07	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007251)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51340
fragaria_vesca	FvH4_3g41130 FvH4_3g41130 FvH4_3g41130 FvH4_3g41130 FvH4_3g41130
malus_domestica	MD03G1047100.v1.1
prunus_persica	Prupe.6G038300_v2.0.a1 Prupe.6G038300_v2.0.a1 Prupe.6G038300_v2.0.a1 Prupe.6G038300_v2.0.a1
pyrus_communis	pycom03g03710
rosa_chinensis	RchiOBHm_Chr5g0073921
rosa_laevigata	RLG00000036425
rosa_multiflora	Rmu_co8214482.1_g000001 Rmu_sc0000470.1_g000018 Rmu_sc0038496.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007450
rosa_rugosa	Rorug05G0427400 Rorug05G0427500
rosa_samantha	Rh5AG484400 Rh5BG505100 Rh5CG528900 Rh5DG517400
rosa_wichuraiana	Rw5G045030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	992
AccB1I	GGYRCC	1 cut(s)	3410
AccB7I	CCANNNNNTGG	1 cut(s)	1412
AccBSI	CCGCTC	2 cut(s)	561, 2463
AccI	GTMKAC	1 cut(s)	3418
AccII	CGCG	3 cut(s)	230, 501, 3720
AciI	CCGC	9 cut(s)	561, 696, 2461, 2700, 3077, 3308, 3351, 3718, 3800
AclWI	GGATC	6 cut(s)	344, 992, 1603, 3365, 3863, 3876
AcoI	YGGCCR	1 cut(s)	474
AcuI	CTGAAG	5 cut(s)	1469, 1601, 2042, 3573, 3680
AcvI	CACGTG	1 cut(s)	3998
AcyI	GRCGYC	1 cut(s)	2020
AdeI	CACNNNGTG	1 cut(s)	3721
AfaI	GTAC	3 cut(s)	565, 2964, 3635
AfeI	AGCGCT	1 cut(s)	413
AfiI	CCNNNNNNNGG	4 cut(s)	85, 1412, 2527, 3391
AflII	CTTAAG	1 cut(s)	1076
AgeI	ACCGGT	1 cut(s)	2983
AhdI	GACNNNNNGTC	2 cut(s)	1666, 2635
AjiI	CACGTC	1 cut(s)	1567
AjnI	CCWGG	7 cut(s)	201, 266, 352, 998, 1531, 2465, 3223
AjuI	GAANNNNNNNTTGG	6 cut(s)	241, 273, 946, 978, 3770, 3802
Alw21I	GWGCWC	6 cut(s)	1024, 1426, 1713, 1756, 2122, 2614
Alw26I	GTCTC	3 cut(s)	1916, 3681, 4320
Alw44I	GTGCAC	1 cut(s)	2118
AlwI	GGATC	6 cut(s)	344, 992, 1603, 3365, 3863, 3876
Ama87I	CYCGRG	3 cut(s)	244, 468, 3550
Aor51HI	AGCGCT	1 cut(s)	413
AoxI	GGCC	3 cut(s)	474, 1165, 1723
ApaLI	GTGCAC	1 cut(s)	2118
ArsI	GACNNNNNNTTYG	2 cut(s)	3188, 3220
AseI	ATTAAT	1 cut(s)	2871
AsiGI	ACCGGT	1 cut(s)	2983
Asp700I	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2838, 3338, 3693, 3738
AspLEI	GCGC	3 cut(s)	232, 414, 2880
AspS9I	GGNCC	4 cut(s)	931, 1165, 3014, 3221
AsuHPI	GGTGA	5 cut(s)	95, 139, 1384, 1811, 2998
AsuII	TTCGAA	1 cut(s)	1432
AvaI	CYCGRG	3 cut(s)	244, 468, 3550
AvaII	GGWCC	3 cut(s)	931, 3014, 3221
BaeGI	GKGCMC	2 cut(s)	2122, 3415
BaeI	ACNNNNGTAYC	2 cut(s)	1568, 1601
BalI	TGGCCA	1 cut(s)	476
BamHI	GGATCC	1 cut(s)	3868
BanI	GGYRCC	1 cut(s)	3410
BanII	GRGCYC	2 cut(s)	1756, 2614
BauI	CACGAG	2 cut(s)	3018, 3248
BbrPI	CACGTG	1 cut(s)	3998
BbsI	GAAGAC	1 cut(s)	2501
Bbv12I	GWGCWC	6 cut(s)	1024, 1426, 1713, 1756, 2122, 2614
BbvCI	CCTCAGC	1 cut(s)	3525
BceAI	ACGGC	3 cut(s)	2505, 3664, 3827
BcgI	CGANNNNNNTGC	4 cut(s)	866, 900, 3239, 3273
BciT130I	CCWGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
BciVI	GTATCC	1 cut(s)	82
BclI	TGATCA	2 cut(s)	1108, 2824
BcoDI	GTCTC	3 cut(s)	1916, 3681, 4320
BfaI	CTAG	3 cut(s)	128, 792, 2280
BfmI	CTRYAG	5 cut(s)	648, 1449, 2193, 3769, 4321
BfoI	RGCGCY	1 cut(s)	415
BfrI	CTTAAG	1 cut(s)	1076
BfuI	GTATCC	1 cut(s)	82
BglII	AGATCT	1 cut(s)	823
BmcAI	AGTACT	1 cut(s)	3635
Bme1390I	CCNGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
Bme18I	GGWCC	3 cut(s)	931, 3014, 3221
BmeRI	GACNNNNNGTC	2 cut(s)	1666, 2635
BmeT110I	CYCGRG	3 cut(s)	244, 468, 3550
BmgBI	CACGTC	1 cut(s)	1567
BmgT120I	GGNCC	4 cut(s)	931, 1165, 3014, 3221
BmiI	GGNNCC	3 cut(s)	3412, 3784, 3870
BmrFI	CCNGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
BmrI	ACTGGG	1 cut(s)	3288
BmsI	GCATC	6 cut(s)	1461, 1591, 2498, 3319, 3945, 4169
BmuI	ACTGGG	1 cut(s)	3288
BpiI	GAAGAC	1 cut(s)	2501
BpmI	CTGGAG	1 cut(s)	2449
Bpu10I	CCTNAGC	2 cut(s)	1622, 3525
Bpu14I	TTCGAA	1 cut(s)	1432
BpuEI	CTTGAG	3 cut(s)	1617, 1973, 2111
Bsa29I	ATCGAT	1 cut(s)	4328
BsaAI	YACGTR	1 cut(s)	3998
BsaBI	GATNNNNATC	2 cut(s)	348, 2823
BsaHI	GRCGYC	1 cut(s)	2020
BsaJI	CCNNGG	6 cut(s)	79, 999, 1532, 2521, 3213, 3786
BsaWI	WCCGGW	2 cut(s)	374, 2983
Bsc4I	CCNNNNNNNGG	4 cut(s)	85, 1412, 2527, 3391
Bse118I	RCCGGY	2 cut(s)	1558, 2983
Bse1I	ACTGG	5 cut(s)	2207, 2233, 3165, 3283, 3886
Bse3DI	GCAATG	4 cut(s)	841, 2254, 3620, 4154
Bse8I	GATNNNNATC	2 cut(s)	348, 2823
BseBI	CCWGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
BseCI	ATCGAT	1 cut(s)	4328
BseDI	CCNNGG	6 cut(s)	79, 999, 1532, 2521, 3213, 3786
BseJI	GATNNNNATC	2 cut(s)	348, 2823
BseLI	CCNNNNNNNGG	4 cut(s)	85, 1412, 2527, 3391
BseMI	GCAATG	4 cut(s)	841, 2254, 3620, 4154
BseNI	ACTGG	5 cut(s)	2207, 2233, 3165, 3283, 3886
BseRI	GAGGAG	3 cut(s)	10, 13, 3635
BseSI	GKGCMC	2 cut(s)	2122, 3415
BsgI	GTGCAG	3 cut(s)	1263, 2491, 4103
Bsh1236I	CGCG	3 cut(s)	230, 501, 3720
BshFI	GGCC	3 cut(s)	476, 1167, 1725
BshNI	GGYRCC	1 cut(s)	3410
BshTI	ACCGGT	1 cut(s)	2983
BshVI	ATCGAT	1 cut(s)	4328
BsiHKAI	GWGCWC	6 cut(s)	1024, 1426, 1713, 1756, 2122, 2614
BsiHKCI	CYCGRG	3 cut(s)	244, 468, 3550
BsiSI	CCGG	3 cut(s)	375, 1559, 2984
BslFI	GGGAC	4 cut(s)	19, 1016, 1928, 3945
BslI	CCNNNNNNNGG	4 cut(s)	85, 1412, 2527, 3391
BsmAI	GTCTC	3 cut(s)	1916, 3681, 4320
BsmBI	CGTCTC	1 cut(s)	3681
BsmFI	GGGAC	4 cut(s)	19, 1016, 1928, 3945
BsmI	GAATGC	1 cut(s)	3340
BsnI	GGCC	3 cut(s)	476, 1167, 1725
BsoBI	CYCGRG	3 cut(s)	244, 468, 3550
Bsp119I	TTCGAA	1 cut(s)	1432
Bsp1286I	GDGCHC	7 cut(s)	1024, 1426, 1713, 1756, 2122, 2614, 3415
Bsp19I	CCATGG	1 cut(s)	3213
BspACI	CCGC	9 cut(s)	561, 696, 2461, 2700, 3077, 3308, 3351, 3718, 3800
BspANI	GGCC	3 cut(s)	476, 1167, 1725
BspDI	ATCGAT	1 cut(s)	4328
BspFNI	CGCG	3 cut(s)	230, 501, 3720
BspHI	TCATGA	1 cut(s)	2947
BspLI	GGNNCC	3 cut(s)	3412, 3784, 3870
BspMAI	CTGCAG	1 cut(s)	3773
BspPI	GGATC	6 cut(s)	344, 992, 1603, 3365, 3863, 3876
BspQI	GCTCTTC	2 cut(s)	159, 402
BspT104I	TTCGAA	1 cut(s)	1432
BspT107I	GGYRCC	1 cut(s)	3410
BspTI	CTTAAG	1 cut(s)	1076
BsrBI	CCGCTC	2 cut(s)	561, 2463
BsrDI	GCAATG	4 cut(s)	841, 2254, 3620, 4154
BsrFI	RCCGGY	2 cut(s)	1558, 2983
BsrI	ACTGG	5 cut(s)	2207, 2233, 3165, 3283, 3886
BssAI	RCCGGY	2 cut(s)	1558, 2983
BssECI	CCNNGG	6 cut(s)	79, 999, 1532, 2521, 3213, 3786
BssNI	GRCGYC	1 cut(s)	2020
BssSI	CACGAG	2 cut(s)	3018, 3248
BssT1I	CCWWGG	2 cut(s)	3213, 3786
Bst2BI	CACGAG	2 cut(s)	3018, 3248
Bst2UI	CCWGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
Bst6I	CTCTTC	4 cut(s)	40, 159, 402, 2376
BstACI	GRCGYC	1 cut(s)	2020
BstAFI	CTTAAG	1 cut(s)	1076
BstAPI	GCANNNNNTGC	1 cut(s)	1480
BstBAI	YACGTR	1 cut(s)	3998
BstBI	TTCGAA	1 cut(s)	1432
BstC8I	GCNNGC	2 cut(s)	942, 1628
BstDSI	CCRYGG	2 cut(s)	2521, 3213
BstFNI	CGCG	3 cut(s)	230, 501, 3720
BstH2I	RGCGCY	1 cut(s)	415
BstHHI	GCGC	3 cut(s)	232, 414, 2880
BstMAI	GTCTC	3 cut(s)	1916, 3681, 4320
BstMWI	GCNNNNNNNGC	7 cut(s)	309, 398, 702, 1480, 2469, 2719, 3839
BstNI	CCWGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
BstNSI	RCATGY	3 cut(s)	1344, 2638, 3840
BstSCI	CCNGG	7 cut(s)	201, 266, 352, 998, 1531, 2465, 3223
BstSFI	CTRYAG	5 cut(s)	648, 1449, 2193, 3769, 4321
BstSLI	GKGCMC	2 cut(s)	2122, 3415
BstUI	CGCG	3 cut(s)	230, 501, 3720
BstV2I	GAAGAC	1 cut(s)	2501
BstX2I	RGATCY	3 cut(s)	349, 823, 3868
BstXI	CCANNNNNNTGG	1 cut(s)	3278
BstYI	RGATCY	3 cut(s)	349, 823, 3868
Bsu15I	ATCGAT	1 cut(s)	4328
BsuI	GTATCC	1 cut(s)	82
BsuRI	GGCC	3 cut(s)	476, 1167, 1725
BsuTUI	ATCGAT	1 cut(s)	4328
BtgI	CCRYGG	2 cut(s)	2521, 3213
BtrI	CACGTC	1 cut(s)	1567
BtsI	GCAGTG	3 cut(s)	2479, 2492, 3778
BtsIMutI	CAGTG	7 cut(s)	2214, 2479, 2492, 3353, 3778, 3868, 3939
Cac8I	GCNNGC	2 cut(s)	942, 1628
CciI	TCATGA	1 cut(s)	2947
CfoI	GCGC	3 cut(s)	232, 414, 2880
Cfr10I	RCCGGY	2 cut(s)	1558, 2983
Cfr13I	GGNCC	4 cut(s)	931, 1165, 3014, 3221
ClaI	ATCGAT	1 cut(s)	4328
CseI	GACGC	4 cut(s)	1670, 2028, 2210, 3942
CsiI	ACCWGGT	1 cut(s)	266
Csp6I	GTAC	3 cut(s)	564, 2963, 3634
CspAI	ACCGGT	1 cut(s)	2983
CspCI	CAANNNNNGTGG	2 cut(s)	1813, 1848
CviQI	GTAC	3 cut(s)	564, 2963, 3634
DraI	TTTAAA	1 cut(s)	3597
DraIII	CACNNNGTG	1 cut(s)	3721
DriI	GACNNNNNGTC	2 cut(s)	1666, 2635
EaeI	YGGCCR	1 cut(s)	474
Eam1104I	CTCTTC	4 cut(s)	40, 159, 402, 2376
Eam1105I	GACNNNNNGTC	2 cut(s)	1666, 2635
EarI	CTCTTC	4 cut(s)	40, 159, 402, 2376
Ecl136II	GAGCTC	2 cut(s)	1754, 2612
Eco130I	CCWWGG	2 cut(s)	3213, 3786
Eco24I	GRGCYC	2 cut(s)	1756, 2614
Eco32I	GATATC	4 cut(s)	1270, 1772, 2077, 2945
Eco47I	GGWCC	3 cut(s)	931, 3014, 3221
Eco47III	AGCGCT	1 cut(s)	413
Eco53kI	GAGCTC	2 cut(s)	1754, 2612
Eco57I	CTGAAG	5 cut(s)	1469, 1601, 2042, 3573, 3680
Eco72I	CACGTG	1 cut(s)	3998
Eco88I	CYCGRG	3 cut(s)	244, 468, 3550
EcoICRI	GAGCTC	2 cut(s)	1754, 2612
EcoO109I	RGGNCCY	2 cut(s)	1165, 3221
EcoRI	GAATTC	2 cut(s)	2274, 3753
EcoRII	CCWGG	7 cut(s)	201, 266, 352, 998, 1531, 2465, 3223
EcoRV	GATATC	4 cut(s)	1270, 1772, 2077, 2945
EcoT14I	CCWWGG	2 cut(s)	3213, 3786
EcoT22I	ATGCAT	1 cut(s)	1476
EcoT38I	GRGCYC	2 cut(s)	1756, 2614
ErhI	CCWWGG	2 cut(s)	3213, 3786
Esp3I	CGTCTC	1 cut(s)	3681
FaqI	GGGAC	4 cut(s)	19, 1016, 1928, 3945
FauI	CCCGC	3 cut(s)	689, 3084, 3807
FauNDI	CATATG	3 cut(s)	58, 2998, 3887
FbaI	TGATCA	2 cut(s)	1108, 2824
FblI	GTMKAC	1 cut(s)	3418
FriOI	GRGCYC	2 cut(s)	1756, 2614
FspBI	CTAG	3 cut(s)	128, 792, 2280
GlaI	GCGC	3 cut(s)	231, 413, 2879
GsuI	CTGGAG	1 cut(s)	2449
HaeII	RGCGCY	1 cut(s)	415
HaeIII	GGCC	3 cut(s)	476, 1167, 1725
HapII	CCGG	3 cut(s)	375, 1559, 2984
HgaI	GACGC	4 cut(s)	1670, 2028, 2210, 3942
HhaI	GCGC	3 cut(s)	232, 414, 2880
Hin1I	GRCGYC	1 cut(s)	2020
Hin6I	GCGC	3 cut(s)	230, 412, 2878
HinP1I	GCGC	3 cut(s)	230, 412, 2878
HincII	GTYRAC	1 cut(s)	264
HindII	GTYRAC	1 cut(s)	264
HindIII	AAGCTT	5 cut(s)	723, 1079, 1811, 2711, 2762
HpaII	CCGG	3 cut(s)	375, 1559, 2984
HphI	GGTGA	5 cut(s)	95, 139, 1384, 1811, 2998
Hpy166II	GTNNAC	5 cut(s)	264, 1819, 2120, 2981, 3419
Hpy8I	GTNNAC	5 cut(s)	264, 1819, 2120, 2981, 3419
Hpy99I	CGWCG	1 cut(s)	891
HpyCH4IV	ACGT	3 cut(s)	63, 1566, 3997
HpyF10VI	GCNNNNNNNGC	7 cut(s)	309, 398, 702, 1480, 2469, 2719, 3839
HpySE526I	ACGT	3 cut(s)	63, 1566, 3997
Hsp92I	GRCGYC	1 cut(s)	2020
HspAI	GCGC	3 cut(s)	230, 412, 2878
Ksp22I	TGATCA	2 cut(s)	1108, 2824
LguI	GCTCTTC	2 cut(s)	159, 402
LmnI	GCTCC	3 cut(s)	1716, 2468, 2609
LweI	GCATC	6 cut(s)	1461, 1591, 2498, 3319, 3945, 4169
MabI	ACCWGGT	1 cut(s)	266
MaeI	CTAG	3 cut(s)	128, 792, 2280
MaeII	ACGT	3 cut(s)	63, 1566, 3997
MbiI	CCGCTC	2 cut(s)	561, 2463
MfeI	CAATTG	7 cut(s)	935, 1890, 2214, 2793, 3009, 3285, 3375
MflI	RGATCY	3 cut(s)	349, 823, 3868
MhlI	GDGCHC	7 cut(s)	1024, 1426, 1713, 1756, 2122, 2614, 3415
MlsI	TGGCCA	1 cut(s)	476
MluNI	TGGCCA	1 cut(s)	476
MlyI	GAGTC	6 cut(s)	460, 521, 706, 1898, 2118, 3542
MmeI	TCCRAC	6 cut(s)	595, 909, 1070, 1365, 2223, 2367
Mox20I	TGGCCA	1 cut(s)	476
Mph1103I	ATGCAT	1 cut(s)	1476
MroXI	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2838, 3338, 3693, 3738
MscI	TGGCCA	1 cut(s)	476
MslI	CAYNNNNRTG	2 cut(s)	117, 585
Msp20I	TGGCCA	1 cut(s)	476
MspCI	CTTAAG	1 cut(s)	1076
MspI	CCGG	3 cut(s)	375, 1559, 2984
MspR9I	CCNGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
MunI	CAATTG	7 cut(s)	935, 1890, 2214, 2793, 3009, 3285, 3375
Mva1269I	GAATGC	1 cut(s)	3340
MvaI	CCWGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
MvnI	CGCG	3 cut(s)	230, 501, 3720
MwoI	GCNNNNNNNGC	7 cut(s)	309, 398, 702, 1480, 2469, 2719, 3839
NcoI	CCATGG	1 cut(s)	3213
NdeI	CATATG	3 cut(s)	58, 2998, 3887
NlaIV	GGNNCC	3 cut(s)	3412, 3784, 3870
NmuCI	GTSAC	6 cut(s)	1040, 1372, 1520, 1658, 2805, 4002
NsiI	ATGCAT	1 cut(s)	1476
NspI	RCATGY	3 cut(s)	1344, 2638, 3840
NspV	TTCGAA	1 cut(s)	1432
PaeR7I	CTCGAG	3 cut(s)	244, 468, 3550
PagI	TCATGA	1 cut(s)	2947
PciSI	GCTCTTC	2 cut(s)	159, 402
PcsI	WCGNNNNNNNCGW	1 cut(s)	3654
PctI	GAATGC	1 cut(s)	3340
PdmI	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2838, 3338, 3693, 3738
PflFI	GACNNNGTC	2 cut(s)	3280, 3673
PflMI	CCANNNNNTGG	1 cut(s)	1412
PfoI	TCCNGGA	1 cut(s)	201
PinAI	ACCGGT	1 cut(s)	2983
PleI	GAGTC	6 cut(s)	460, 520, 706, 1898, 2118, 3542
PmaCI	CACGTG	1 cut(s)	3998
PmlI	CACGTG	1 cut(s)	3998
PpsI	GAGTC	6 cut(s)	460, 520, 706, 1898, 2118, 3542
Ppu21I	YACGTR	1 cut(s)	3998
PpuMI	RGGWCCY	1 cut(s)	3221
PshBI	ATTAAT	1 cut(s)	2871
PsiI	TTATAA	1 cut(s)	992
Psp124BI	GAGCTC	2 cut(s)	1756, 2614
Psp5II	RGGWCCY	1 cut(s)	3221
Psp6I	CCWGG	7 cut(s)	201, 266, 352, 998, 1531, 2465, 3223
PspCI	CACGTG	1 cut(s)	3998
PspGI	CCWGG	7 cut(s)	201, 266, 352, 998, 1531, 2465, 3223
PspN4I	GGNNCC	3 cut(s)	3412, 3784, 3870
PspPI	GGNCC	4 cut(s)	931, 1165, 3014, 3221
PspPPI	RGGWCCY	1 cut(s)	3221
PspXI	VCTCGAGB	2 cut(s)	468, 3550
PsrI	GAACNNNNNNTAC	6 cut(s)	2886, 2918, 4214, 4246, 4282, 4314
PstI	CTGCAG	1 cut(s)	3773
PsuI	RGATCY	3 cut(s)	349, 823, 3868
PsyI	GACNNNGTC	2 cut(s)	3280, 3673
RsaI	GTAC	3 cut(s)	565, 2964, 3635
RsaNI	GTAC	3 cut(s)	564, 2963, 3634
RseI	CAYNNNNRTG	2 cut(s)	117, 585
SacI	GAGCTC	2 cut(s)	1756, 2614
SapI	GCTCTTC	2 cut(s)	159, 402
Sau96I	GGNCC	4 cut(s)	931, 1165, 3014, 3221
ScaI	AGTACT	1 cut(s)	3635
SchI	GAGTC	6 cut(s)	460, 521, 706, 1898, 2118, 3542
ScrFI	CCNGG	7 cut(s)	203, 268, 354, 1000, 1533, 2467, 3225
SduI	GDGCHC	7 cut(s)	1024, 1426, 1713, 1756, 2122, 2614, 3415
SexAI	ACCWGGT	1 cut(s)	266
SfaNI	GCATC	6 cut(s)	1461, 1591, 2498, 3319, 3945, 4169
SfcI	CTRYAG	5 cut(s)	648, 1449, 2193, 3769, 4321
Sfr274I	CTCGAG	3 cut(s)	244, 468, 3550
SfuI	TTCGAA	1 cut(s)	1432
SinI	GGWCC	3 cut(s)	931, 3014, 3221
SlaI	CTCGAG	3 cut(s)	244, 468, 3550
SmiMI	CAYNNNNRTG	2 cut(s)	117, 585
SmlI	CTYRAG	7 cut(s)	244, 468, 1076, 1632, 1952, 2126, 3550
SmoI	CTYRAG	7 cut(s)	244, 468, 1076, 1632, 1952, 2126, 3550
SsiI	CCGC	9 cut(s)	561, 696, 2461, 2700, 3077, 3308, 3351, 3718, 3800
SspI	AATATT	4 cut(s)	1326, 1380, 2266, 3028
SspMI	CTAG	3 cut(s)	128, 792, 2280
SstI	GAGCTC	2 cut(s)	1756, 2614
StyD4I	CCNGG	7 cut(s)	201, 266, 352, 998, 1531, 2465, 3223
StyI	CCWWGG	2 cut(s)	3213, 3786
TaiI	ACGT	3 cut(s)	66, 1569, 4000
TaqII	GACCGA	1 cut(s)	147
TatI	WGTACW	1 cut(s)	3633
TscAI	CASTG	7 cut(s)	2214, 2479, 2499, 3353, 3778, 3868, 3946
TseFI	GTSAC	6 cut(s)	1040, 1372, 1520, 1658, 2805, 4002
Tsp45I	GTSAC	6 cut(s)	1040, 1372, 1520, 1658, 2805, 4002
TspGWI	ACGGA	3 cut(s)	2416, 2606, 2890
TspRI	CASTG	7 cut(s)	2214, 2479, 2499, 3353, 3778, 3868, 3946
Tth111I	GACNNNGTC	2 cut(s)	3280, 3673
Van91I	CCANNNNNTGG	1 cut(s)	1412
Vha464I	CTTAAG	1 cut(s)	1076
VneI	GTGCAC	1 cut(s)	2118
VpaK11BI	GGWCC	3 cut(s)	931, 3014, 3221
VspI	ATTAAT	1 cut(s)	2871
XbaI	TCTAGA	2 cut(s)	127, 791
XceI	RCATGY	3 cut(s)	1344, 2638, 3840
XhoI	CTCGAG	3 cut(s)	244, 468, 3550
XmiI	GTMKAC	1 cut(s)	3418
XmnI	GAANNNNTTC	7 cut(s)	1097, 1506, 1862, 2838, 3338, 3693, 3738
XspI	CTAG	3 cut(s)	128, 792, 2280
ZrmI	AGTACT	1 cut(s)	3635
Zsp2I	ATGCAT	1 cut(s)	1476

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.