MD03G1059200.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012787
Physical Location & Seq
Forward (+)
4774413 .. 4776918
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1059200.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGCAACAGCATATTCTCAAACCCTTGTTCATTCAAATATTCAATTACCTTTTGAAATTTGAAACAATGGCTTCCAATAATACTTTTTCTTTGTGTTCCATATTGGTGATTGTCACACTCGGTGTTCTTGTGGGTTTCACGGGGAACTCTTCTGCTCAACTTTCTACGAATTTTTATTCTAAGAGTTGTCCTCAAGTCTTTGATGCAGTCAAGTCTGTTGTCAAGTCTGCTGTGTCTAAAGAAAAGCGCATGGGCGCTTCTCTTCTTCGCCTCCACTTTCATGACTGTTTCGTCAACGGTTGTGATGGATCGCTATTACTGGACGACACTTCTTCCTTCACCGGTGAGAAGACTGCACTCCCGAATAACAACTCCGTAAGAGGATTTGAGGTTGTGGACAAGATCAAGTCCCAAGTGGAAAAGGCATGCCCCGGCGTCGTTTCCTGTGCTGATATCTTAGCCATTGCTTCTCGAGACTCCGTCCAAATTCTTGGAGGACCAAGTTGGGAAGTTAAACTTGGAAGAAGGGATTCAAAGACAGCAAGCCTATCGGCTGCCAATAGTGGAGTCCTCCCTCCGCCCACTGCTACCCTCAACGAGCTCACCACTAGATTCCGAGCCGTAGGTCTCTCTCAAAGGGACTTGGTAGCCTTATCTGGAGCTCACACAATTGGGCAAGCAAGGTGTACAACTTTCAGAGCTCGCATATATAACGAGACCAACATTGATGCTTCATTTGCCAGGACCAGGCAAAACACATGTCCAAGAACAGCTGGCTCCGGAGACGACAACCTTGCACCTTTTGACATCACTACTCCTAACACTTTCGACACTGCCTACTTCAAGAACCTTGTCAACCAAAAGGGTCTCCTCCACAGTGACCAAATTTTGTTTAATGGTGGACCAACCGATTCATTGGTTAAATCGTACAGTGGCAGTGCTAACACCTTCAATGCTGACTTTGCCAAGGCAATGATCAAGATGGGAGATAACAAGCCACTCACTGGATCCAAGGGTGAGATTAGATTGAACTGCAGGAAGCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

350

Amino Acids

37.7

Weight (kDa)

9.19

Isoelectric Point (pI)

27.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 70 - 311 1.2e-86 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58390 AT5G58400
fragaria_vesca FvH4_3g40180
malus_domestica MD03G1059200.v1.1 MD11G1061400.v1.1
prunus_persica Prupe.6G044000_v2.0.a1 Prupe.6G044300_v2.0.a1
pyrus_communis pycom03g04760 pycom11g05100
rosa_chinensis RchiOBHm_Chr5g0072371
rosa_laevigata RLG00000036295
rosa_multiflora Rmu_sc0000737.1_g000014
rosa_roxburghii Rroxscaffold_1G00008850
rosa_rugosa Rorug05G0417600
rosa_samantha Rh5AG474200 Rh5CG518300 Rh5DG506400
rosa_wichuraiana Rw5G044030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccIII TCCGGA 1 cut(s) 779
AciI CCGC 1 cut(s) 578
AclWI GGATC 3 cut(s) 316, 1002, 1015
AcsI RAATTY 4 cut(s) 56, 169, 486, 885
AcyI GRCGYC 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 122
AfaI GTAC 2 cut(s) 688, 929
AfiI CCNNNNNNNGG 2 cut(s) 747, 1004
AflIII ACRYGT 1 cut(s) 758
AgeI ACCGGT 1 cut(s) 341
AgsI TTSAA 8 cut(s) 35, 43, 55, 62, 534, 844, 952, 1030
AjnI CCWGG 2 cut(s) 740, 746
AjuI GAANNNNNNNTTGG 2 cut(s) 501, 533
AluBI AGCT 4 cut(s) 601, 662, 701, 773
AluI AGCT 4 cut(s) 601, 662, 701, 773
Alw21I GWGCWC 3 cut(s) 603, 664, 703
Alw26I GTCTC 5 cut(s) 468, 632, 710, 777, 872
AlwI GGATC 3 cut(s) 316, 1002, 1015
Ama87I CYCGRG 1 cut(s) 471
Aor13HI TCCGGA 1 cut(s) 779
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 4 cut(s) 56, 169, 486, 885
ArsI GACNNNNNNTTYG 2 cut(s) 745, 777
AsiGI ACCGGT 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 529
AspLEI GCGC 2 cut(s) 249, 257
AspS9I GGNCC 3 cut(s) 497, 744, 902
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 5 cut(s) 118, 331, 356, 595, 1028
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 3 cut(s) 497, 744, 902
BamHI GGATCC 1 cut(s) 1007
BanII GRGCYC 3 cut(s) 603, 664, 703
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 3 cut(s) 603, 664, 703
BbvI GCAGC 1 cut(s) 541
BccI CCATC 2 cut(s) 299, 976
BceAI ACGGC 1 cut(s) 605
BcgI CGANNNNNNTGC 4 cut(s) 531, 565, 776, 810
BciT130I CCWGG 2 cut(s) 742, 748
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 1 cut(s) 432
BcoDI GTCTC 5 cut(s) 468, 632, 710, 777, 872
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 1033
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 556
Bme1390I CCNGG 3 cut(s) 432, 742, 748
Bme18I GGWCC 3 cut(s) 497, 744, 902
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 3 cut(s) 497, 744, 902
BmiI GGNNCC 2 cut(s) 778, 1009
BmrFI CCNGG 3 cut(s) 432, 742, 748
BmsI GCATC 2 cut(s) 193, 718
BpiI GAAGAC 1 cut(s) 356
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 3 cut(s) 632, 710, 872
BsaJI CCNNGG 3 cut(s) 430, 966, 1011
BsaWI WCCGGW 2 cut(s) 341, 779
Bsc4I CCNNNNNNNGG 2 cut(s) 747, 1004
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 324, 1009
Bse3DI GCAATG 2 cut(s) 462, 978
BseAI TCCGGA 1 cut(s) 779
BseBI CCWGG 2 cut(s) 742, 748
BseDI CCNNGG 3 cut(s) 430, 966, 1011
BseLI CCNNNNNNNGG 2 cut(s) 747, 1004
BseMI GCAATG 2 cut(s) 462, 978
BseNI ACTGG 2 cut(s) 324, 1009
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 339
BshTI ACCGGT 1 cut(s) 341
BsiHKAI GWGCWC 3 cut(s) 603, 664, 703
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 3 cut(s) 342, 432, 780
BslFI GGGAC 2 cut(s) 394, 653
BslI CCNNNNNNNGG 2 cut(s) 747, 1004
BsmAI GTCTC 5 cut(s) 468, 632, 710, 777, 872
BsmBI CGTCTC 1 cut(s) 777
BsmFI GGGAC 2 cut(s) 394, 653
Bso31I GGTCTC 3 cut(s) 632, 710, 872
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 3 cut(s) 603, 664, 703
Bsp13I TCCGGA 1 cut(s) 779
Bsp1407I TGTACA 1 cut(s) 686
Bsp143I GATC 4 cut(s) 308, 402, 975, 1007
BspACI CCGC 1 cut(s) 578
BspEI TCCGGA 1 cut(s) 779
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 778, 1009
BspMAI CTGCAG 1 cut(s) 1037
BspPI GGATC 3 cut(s) 316, 1002, 1015
BspTNI GGTCTC 3 cut(s) 632, 710, 872
BsrDI GCAATG 2 cut(s) 462, 978
BsrFI RCCGGY 1 cut(s) 341
BsrGI TGTACA 1 cut(s) 686
BsrI ACTGG 2 cut(s) 324, 1009
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 430, 966, 1011
BssMI GATC 4 cut(s) 308, 402, 975, 1007
BssNI GRCGYC 1 cut(s) 435
BssT1I CCWWGG 2 cut(s) 966, 1011
Bst2UI CCWGG 2 cut(s) 742, 748
Bst4CI ACNGT 4 cut(s) 287, 299, 878, 932
Bst6I CTCTTC 2 cut(s) 154, 267
BstACI GRCGYC 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 686
BstC8I GCNNGC 5 cut(s) 427, 544, 678, 703, 775
BstDEI CTNAG 2 cut(s) 180, 457
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 249, 257
BstKTI GATC 4 cut(s) 311, 405, 978, 1010
BstMAI GTCTC 5 cut(s) 468, 632, 710, 777, 872
BstMBI GATC 4 cut(s) 308, 402, 975, 1007
BstMWI GCNNNNNNNGC 2 cut(s) 737, 962
BstNI CCWGG 2 cut(s) 742, 748
BstNSI RCATGY 2 cut(s) 429, 762
BstSCI CCNGG 3 cut(s) 430, 740, 746
BstSFI CTRYAG 1 cut(s) 1033
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 1 cut(s) 1007
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 1007
BtsI GCAGTG 3 cut(s) 582, 831, 943
BtsIMutI CAGTG 6 cut(s) 582, 831, 883, 937, 943, 1002
Cac8I GCNNGC 5 cut(s) 427, 544, 678, 703, 775
CciI TCATGA 1 cut(s) 280
CfoI GCGC 2 cut(s) 249, 257
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 497, 744, 902
CseI GACGC 1 cut(s) 424
Csp6I GTAC 2 cut(s) 687, 928
CspAI ACCGGT 1 cut(s) 341
CviAII CATG 4 cut(s) 250, 281, 426, 759
CviQI GTAC 2 cut(s) 687, 928
DdeI CTNAG 2 cut(s) 180, 457
DpnI GATC 4 cut(s) 310, 404, 977, 1009
DpnII GATC 4 cut(s) 308, 402, 975, 1007
DraIII CACNNNGTG 1 cut(s) 122
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
Eam1104I CTCTTC 2 cut(s) 154, 267
EarI CTCTTC 2 cut(s) 154, 267
EciI GGCGGA 1 cut(s) 567
Ecl136II GAGCTC 3 cut(s) 601, 662, 701
Eco130I CCWWGG 2 cut(s) 966, 1011
Eco24I GRGCYC 3 cut(s) 603, 664, 703
Eco31I GGTCTC 3 cut(s) 632, 710, 872
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 3 cut(s) 497, 744, 902
Eco53kI GAGCTC 3 cut(s) 601, 662, 701
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 3 cut(s) 601, 662, 701
EcoRII CCWGG 2 cut(s) 740, 746
EcoRV GATATC 1 cut(s) 454
EcoT14I CCWWGG 2 cut(s) 966, 1011
EcoT38I GRGCYC 3 cut(s) 603, 664, 703
ErhI CCWWGG 2 cut(s) 966, 1011
Esp3I CGTCTC 1 cut(s) 777
FaeI CATG 4 cut(s) 253, 284, 429, 762
FaiI YATR 9 cut(s) 12, 101, 251, 282, 427, 707, 709, 711, 760
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 2 cut(s) 394, 653
FatI CATG 4 cut(s) 249, 280, 425, 758
FbaI TGATCA 1 cut(s) 975
Fnu4HI GCNGC 1 cut(s) 555
FriOI GRGCYC 3 cut(s) 603, 664, 703
Fsp4HI GCNGC 1 cut(s) 555
FspBI CTAG 1 cut(s) 609
GlaI GCGC 2 cut(s) 248, 256
GluI GCNGC 1 cut(s) 555
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 258
HapII CCGG 3 cut(s) 342, 432, 780
HgaI GACGC 1 cut(s) 424
HhaI GCGC 2 cut(s) 249, 257
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 4 cut(s) 253, 284, 429, 762
Hin6I GCGC 2 cut(s) 247, 255
HinP1I GCGC 2 cut(s) 247, 255
HincII GTYRAC 2 cut(s) 295, 856
HindII GTYRAC 2 cut(s) 295, 856
HinfI GANTC 5 cut(s) 476, 530, 567, 612, 911
HpaII CCGG 3 cut(s) 342, 432, 780
HphI GGTGA 5 cut(s) 118, 331, 356, 595, 1028
Hpy166II GTNNAC 5 cut(s) 295, 397, 687, 856, 902
Hpy188I TCNGA 2 cut(s) 617, 698
Hpy188III TCNNGA 8 cut(s) 281, 361, 471, 473, 657, 780, 844, 979
Hpy8I GTNNAC 5 cut(s) 295, 397, 687, 856, 902
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 3 cut(s) 346, 519, 958
HpyCH4III ACNGT 4 cut(s) 287, 299, 878, 932
HpyCH4V TGCA 5 cut(s) 4, 206, 356, 797, 1035
HpyF10VI GCNNNNNNNGC 2 cut(s) 737, 962
HpyF3I CTNAG 2 cut(s) 180, 457
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 4 cut(s) 253, 284, 429, 762
HspAI GCGC 2 cut(s) 247, 255
Kpn2I TCCGGA 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 975
Kzo9I GATC 4 cut(s) 308, 402, 975, 1007
LmnI GCTCC 2 cut(s) 659, 782
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 2 cut(s) 193, 718
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 112, 878
MalI GATC 4 cut(s) 310, 404, 977, 1009
MboI GATC 4 cut(s) 308, 402, 975, 1007
MboII GAAGA 6 cut(s) 141, 254, 257, 324, 361, 534
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 1 cut(s) 1007
MhlI GDGCHC 3 cut(s) 603, 664, 703
MluCI AATT 7 cut(s) 43, 56, 169, 486, 669, 885, 1045
MlyI GAGTC 2 cut(s) 470, 576
MnlI CCTC 9 cut(s) 201, 281, 374, 382, 488, 581, 585, 602, 881
MroI TCCGGA 1 cut(s) 779
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 3 cut(s) 513, 894, 921
MslI CAYNNNNRTG 2 cut(s) 104, 279
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 3 cut(s) 342, 432, 780
MspR9I CCNGG 3 cut(s) 432, 742, 748
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 2 cut(s) 742, 748
MwoI GCNNNNNNNGC 2 cut(s) 737, 962
NciI CCSGG 1 cut(s) 432
NdeII GATC 4 cut(s) 308, 402, 975, 1007
NlaIII CATG 4 cut(s) 253, 284, 429, 762
NlaIV GGNNCC 2 cut(s) 778, 1009
NmuCI GTSAC 2 cut(s) 112, 878
NspI RCATGY 2 cut(s) 429, 762
PaeI GCATGC 1 cut(s) 429
PaeR7I CTCGAG 1 cut(s) 471
PagI TCATGA 1 cut(s) 280
PciI ACATGT 1 cut(s) 758
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 3 cut(s) 530, 612, 911
PflFI GACNNNGTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 1004
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 1 cut(s) 556
PleI GAGTC 2 cut(s) 470, 575
PpsI GAGTC 2 cut(s) 470, 575
PscI ACATGT 1 cut(s) 758
Psp124BI GAGCTC 3 cut(s) 603, 664, 703
Psp6I CCWGG 2 cut(s) 740, 746
PspGI CCWGG 2 cut(s) 740, 746
PspN4I GGNNCC 2 cut(s) 778, 1009
PspPI GGNCC 3 cut(s) 497, 744, 902
PstI CTGCAG 1 cut(s) 1037
PsuI RGATCY 1 cut(s) 1007
PsyI GACNNNGTC 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 2 cut(s) 688, 929
RsaNI GTAC 2 cut(s) 687, 928
RseI CAYNNNNRTG 2 cut(s) 104, 279
SacI GAGCTC 3 cut(s) 603, 664, 703
SaqAI TTAA 3 cut(s) 513, 894, 921
SatI GCNGC 1 cut(s) 555
Sau3AI GATC 4 cut(s) 308, 402, 975, 1007
Sau96I GGNCC 3 cut(s) 497, 744, 902
SchI GAGTC 2 cut(s) 470, 576
ScrFI CCNGG 3 cut(s) 432, 742, 748
SduI GDGCHC 3 cut(s) 603, 664, 703
SfaNI GCATC 2 cut(s) 193, 718
SfcI CTRYAG 1 cut(s) 1033
Sfr274I CTCGAG 1 cut(s) 471
SgrAI CRCCGGYG 1 cut(s) 341
SinI GGWCC 3 cut(s) 497, 744, 902
SlaI CTCGAG 1 cut(s) 471
SmiMI CAYNNNNRTG 2 cut(s) 104, 279
SmlI CTYRAG 2 cut(s) 192, 471
SmoI CTYRAG 2 cut(s) 192, 471
SphI GCATGC 1 cut(s) 429
Sse9I AATT 7 cut(s) 43, 56, 169, 486, 669, 885, 1045
SsiI CCGC 1 cut(s) 578
SspI AATATT 1 cut(s) 39
SspMI CTAG 1 cut(s) 609
SstI GAGCTC 3 cut(s) 603, 664, 703
StyD4I CCNGG 3 cut(s) 430, 740, 746
StyI CCWWGG 2 cut(s) 966, 1011
TaaI ACNGT 4 cut(s) 287, 299, 878, 932
TaqI TCGA 2 cut(s) 472, 828
TasI AATT 7 cut(s) 43, 56, 169, 486, 669, 885, 1045
TatI WGTACW 1 cut(s) 686
TfiI GAWTC 3 cut(s) 530, 612, 911
Tru1I TTAA 3 cut(s) 513, 894, 921
Tru9I TTAA 3 cut(s) 513, 894, 921
TscAI CASTG 6 cut(s) 589, 838, 883, 937, 943, 1009
TseFI GTSAC 2 cut(s) 112, 878
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 2 cut(s) 112, 878
TspDTI ATGAA 4 cut(s) 19, 269, 723, 903
TspGWI ACGGA 2 cut(s) 364, 469
TspRI CASTG 6 cut(s) 589, 838, 883, 937, 943, 1009
Tth111I GACNNNGTC 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 1004
VpaK11BI GGWCC 3 cut(s) 497, 744, 902
XapI RAATTY 4 cut(s) 56, 169, 486, 885
XceI RCATGY 2 cut(s) 429, 762
XhoI CTCGAG 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.