MD03G1073300.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013422
Physical Location & Seq
Reverse (-)
5973346 .. 5974473
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1073300.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCTAACATATCAACCCTCTTCAACAAGTTCCTCTCTCTCCTCATCCTCATCCTCCACCTCGGCTGTTTCTCTTCCACCGCCACTGCCAACCACCCAAAAAAGCCTCATCACCGCCGTAAACTCTCCACTCACCTCTCCAAACCCAGCACCCTAAAACCCCAAAAAGCCCTCTCCACCTCCTGGTCCTATCTCAAACGCATTTTCACCTCCAAATCCTACAAAATCACCTGCACCAACATCATCCAATCCCACCTCTCCACGCCACCTCGATCCTCCCACCACTCCATCGTCTCCCTCGTCCTACCTGACTCCGACCCCAAATACCTACCTGGGTCGCTCCCCGAATCAGATATCTCGGCCGATTCCCACCAATTGTTCCCTCTCCGAAACGATATCTTCCCCTGCACCGCCTGCGGGGAAATCTTCCCGAAACCCGAGACTCTCGATCACCACCAGGCGATCCACCACGCCGTTTCGGAACTTCATGACGGAGACTCGGGCAAAAACATTGTCCGAATCATATTCAAAACAGGTTGGACCGATACGCGAAAAGCCCCCGAAATTCACCGGATCCTGAAGATCCACAACAGCGGAAAAATCCTGTCGAGGTTCGAGGAGTACAGAGAGCTGGTCAAGTCCAAGGCGGCGCGAAACGGCACCGTTCGGAGGAGGGATGAGCGGTGCATCGCCGACGGCAACGAGCTTCTCAGATTTCACTGCTCAACTTTCGTTTGCGATTTGGGACTCAACGGGAATTCTGGGATTTGTAATCACGAGTACTGCAGTGTTTGTGGAATTATTAAATCTGGATTCTCACCCAAGTTGGACGGAATTTCCACGCTGTCGAGTAGCTCCAGAGCACACGTGGCAATTCCAGAGGATATCGAGGAGGAGTTTCAGTTCATGAACGTGAAGCGGGCTATGCTGGTCTGCCGGGTCGTGGCGGGTCGGGTCGGGTGTGACACTGAGGAGGATATTGACGACGTGGACAAAGAGGGCGGCGGATTCGACTCGGTTGTCGGCAGAGAAGGCAGCGGGGTCCACACGAGGGTGGACGAGGAGGAGCTTTTGGTGTTTAATCCAAGAGCCGTTCTTCCTTGCTTTGTGATCGTATATACCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

376

Amino Acids

41.75

Weight (kDa)

8.19

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29660
fragaria_vesca FvH4_3g38290 FvH4_3g38290
malus_domestica MD03G1073300.v1.1 MD11G1079200.v1.1
prunus_persica Prupe.6G060000_v2.0.a1
pyrus_communis pycom03g05930 pycom11g06650
rosa_chinensis RchiOBHm_Chr5g0068821
rosa_laevigata RLG00000036012
rosa_multiflora Rmu_sc0014317.1_g000001
rosa_roxburghii Rroxscaffold_1G00012020
rosa_rugosa Rorug05G0393400
rosa_samantha Rh5AG450800 Rh5BG469300 Rh5CG491500 Rh5DG482600
rosa_wichuraiana Rw5G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
AasI GACNNNNNNGTC 1 cut(s) 1019
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 183
AccBSI CCGCTC 1 cut(s) 682
AccII CGCG 2 cut(s) 550, 652
AclWI GGATC 5 cut(s) 267, 457, 568, 577, 581
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 3 cut(s) 564, 757, 834
AcuI CTGAAG 1 cut(s) 599
AcvI CACGTG 1 cut(s) 868
AfaI GTAC 2 cut(s) 623, 782
AfiI CCNNNNNNNGG 5 cut(s) 183, 417, 669, 943, 1051
AflIII ACRYGT 1 cut(s) 865
AgsI TTSAA 2 cut(s) 25, 529
AjiI CACGTC 1 cut(s) 988
AjnI CCWGG 3 cut(s) 182, 331, 456
AleI CACNNNNGTG 1 cut(s) 1052
AloI GAACNNNNNNTCC 2 cut(s) 887, 919
AluBI AGCT 4 cut(s) 631, 706, 855, 1069
AluI AGCT 4 cut(s) 631, 706, 855, 1069
Alw21I GWGCWC 1 cut(s) 865
Alw26I GTCTC 3 cut(s) 298, 434, 489
AlwI GGATC 5 cut(s) 267, 457, 568, 577, 581
Ama87I CYCGRG 2 cut(s) 437, 499
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 1 cut(s) 1035
ApoI RAATTY 3 cut(s) 564, 757, 834
ArsI GACNNNNNNTTYG 2 cut(s) 498, 530
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 3 cut(s) 186, 540, 1042
AsuC2I CCSGG 1 cut(s) 938
AsuHPI GGTGA 7 cut(s) 104, 125, 199, 220, 443, 560, 810
AvaI CYCGRG 2 cut(s) 437, 499
AvaII GGWCC 3 cut(s) 186, 540, 1042
BamHI GGATCC 1 cut(s) 573
BanI GGYRCC 1 cut(s) 659
BauI CACGAG 2 cut(s) 776, 1048
BbrPI CACGTG 1 cut(s) 868
Bbv12I GWGCWC 1 cut(s) 865
BbvI GCAGC 1 cut(s) 1047
BccI CCATC 1 cut(s) 296
BceAI ACGGC 5 cut(s) 102, 458, 673, 712, 1076
BciT130I CCWGG 3 cut(s) 184, 333, 458
BcnI CCSGG 1 cut(s) 938
BcoDI GTCTC 3 cut(s) 298, 434, 489
BfmI CTRYAG 1 cut(s) 784
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 3 cut(s) 648, 1003, 1036
BlsI GCNGC 3 cut(s) 649, 1004, 1037
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 4 cut(s) 184, 333, 458, 938
Bme18I GGWCC 3 cut(s) 186, 540, 1042
BmeT110I CYCGRG 2 cut(s) 437, 499
BmgBI CACGTC 1 cut(s) 988
BmgT120I GGNCC 3 cut(s) 186, 540, 1042
BmiI GGNNCC 3 cut(s) 575, 661, 1043
BmrFI CCNGG 4 cut(s) 184, 333, 458, 938
BmsI GCATC 1 cut(s) 696
BpmI CTGGAG 1 cut(s) 841
BpuMI CCSGG 1 cut(s) 938
BsaAI YACGTR 1 cut(s) 868
BsaJI CCNNGG 3 cut(s) 61, 332, 642
BsaWI WCCGGW 1 cut(s) 570
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 5 cut(s) 183, 417, 669, 943, 1051
BseBI CCWGG 3 cut(s) 184, 333, 458
BseDI CCNNGG 3 cut(s) 61, 332, 642
BseGI GGATG 4 cut(s) 45, 51, 243, 682
BseLI CCNNNNNNNGG 5 cut(s) 183, 417, 669, 943, 1051
BseMII CTCAG 2 cut(s) 724, 960
BseRI GAGGAG 8 cut(s) 32, 632, 685, 905, 908, 986, 1076, 1079
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 1 cut(s) 1047
BseYI CCCAGC 1 cut(s) 146
BsgI GTGCAG 2 cut(s) 217, 391
Bsh1236I CGCG 2 cut(s) 550, 652
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 1 cut(s) 659
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 865
BsiHKCI CYCGRG 2 cut(s) 437, 499
BsiSI CCGG 2 cut(s) 571, 937
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 5 cut(s) 183, 417, 669, 943, 1051
BsmAI GTCTC 3 cut(s) 298, 434, 489
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 759
BsnI GGCC 1 cut(s) 362
BsoBI CYCGRG 2 cut(s) 437, 499
Bsp1286I GDGCHC 1 cut(s) 865
Bsp143I GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 2 cut(s) 723, 961
BspFNI CGCG 2 cut(s) 550, 652
BspHI TCATGA 2 cut(s) 487, 906
BspLI GGNNCC 3 cut(s) 575, 661, 1043
BspMAI CTGCAG 1 cut(s) 788
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 5 cut(s) 267, 457, 568, 577, 581
BspT107I GGYRCC 1 cut(s) 659
BsrBI CCGCTC 1 cut(s) 682
BssECI CCNNGG 3 cut(s) 61, 332, 642
BssMI GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
BssSI CACGAG 2 cut(s) 776, 1048
BssT1I CCWWGG 1 cut(s) 642
Bst2BI CACGAG 2 cut(s) 776, 1048
Bst2UI CCWGG 3 cut(s) 184, 333, 458
Bst4CI ACNGT 2 cut(s) 664, 1123
Bst6I CTCTTC 2 cut(s) 26, 79
BstAPI GCANNNNNTGC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 415, 921
BstDEI CTNAG 2 cut(s) 710, 969
BstF5I GGATG 4 cut(s) 45, 51, 243, 682
BstFNI CGCG 2 cut(s) 550, 652
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 6 cut(s) 275, 451, 465, 576, 585, 1113
BstMAI GTCTC 3 cut(s) 298, 434, 489
BstMBI GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 4 cut(s) 414, 869, 925, 1032
BstNI CCWGG 3 cut(s) 184, 333, 458
BstSCI CCNGG 4 cut(s) 182, 331, 456, 936
BstSFI CTRYAG 1 cut(s) 784
BstUI CGCG 2 cut(s) 550, 652
BstV1I GCAGC 1 cut(s) 1047
BstX2I RGATCY 2 cut(s) 573, 582
BstYI RGATCY 2 cut(s) 573, 582
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 1 cut(s) 362
BtgZI GCGATG 1 cut(s) 673
BtrI CACGTC 1 cut(s) 988
BtsCI GGATG 4 cut(s) 45, 51, 243, 682
BtsI GCAGTG 3 cut(s) 84, 718, 793
BtsIMutI CAGTG 4 cut(s) 84, 718, 793, 966
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 2 cut(s) 415, 921
CciI TCATGA 2 cut(s) 487, 906
CfoI GCGC 1 cut(s) 652
Cfr13I GGNCC 3 cut(s) 186, 540, 1042
Csp6I GTAC 2 cut(s) 622, 781
CviAII CATG 2 cut(s) 488, 907
CviQI GTAC 2 cut(s) 622, 781
DdeI CTNAG 2 cut(s) 710, 969
DpnI GATC 6 cut(s) 274, 450, 464, 575, 584, 1112
DpnII GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
DrdI GACNNNNNNGTC 1 cut(s) 1019
DseDI GACNNNNNNGTC 1 cut(s) 1019
EaeI YGGCCR 1 cut(s) 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 26, 79
EarI CTCTTC 2 cut(s) 26, 79
EciI GGCGGA 1 cut(s) 1020
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 642
Eco32I GATATC 3 cut(s) 355, 397, 886
Eco47I GGWCC 3 cut(s) 186, 540, 1042
Eco52I CGGCCG 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 599
Eco72I CACGTG 1 cut(s) 868
Eco88I CYCGRG 2 cut(s) 437, 499
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 3 cut(s) 182, 331, 456
EcoRV GATATC 3 cut(s) 355, 397, 886
EcoT14I CCWWGG 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 642
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 2 cut(s) 491, 910
FaiI YATR 7 cut(s) 11, 489, 524, 908, 926, 1117, 1119
FaqI GGGAC 1 cut(s) 759
FatI CATG 2 cut(s) 487, 906
FauI CCCGC 4 cut(s) 410, 912, 940, 1031
Fnu4HI GCNGC 3 cut(s) 648, 1003, 1036
FokI GGATG 4 cut(s) 32, 38, 230, 689
Fsp4HI GCNGC 3 cut(s) 648, 1003, 1036
GlaI GCGC 1 cut(s) 651
GluI GCNGC 3 cut(s) 648, 1003, 1036
GsaI CCCAGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 841
HaeIII GGCC 1 cut(s) 362
HapII CCGG 2 cut(s) 571, 937
HhaI GCGC 1 cut(s) 652
Hin1II CATG 2 cut(s) 491, 910
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HpaII CCGG 2 cut(s) 571, 937
HphI GGTGA 7 cut(s) 104, 125, 199, 220, 443, 560, 810
Hpy166II GTNNAC 4 cut(s) 122, 991, 1045, 1057
Hpy188I TCNGA 7 cut(s) 316, 352, 389, 481, 518, 669, 713
Hpy188III TCNNGA 9 cut(s) 430, 446, 488, 577, 776, 810, 858, 878, 907
Hpy8I GTNNAC 4 cut(s) 122, 991, 1045, 1057
Hpy99I CGWCG 2 cut(s) 698, 989
HpyAV CCTTC 1 cut(s) 1025
HpyCH4III ACNGT 2 cut(s) 664, 1123
HpyCH4IV ACGT 3 cut(s) 867, 912, 987
HpyCH4V TGCA 4 cut(s) 234, 408, 687, 786
HpyF10VI GCNNNNNNNGC 4 cut(s) 414, 869, 925, 1032
HpyF3I CTNAG 2 cut(s) 710, 969
HpySE526I ACGT 3 cut(s) 867, 912, 987
Hsp92II CATG 2 cut(s) 491, 910
HspAI GCGC 1 cut(s) 650
Kzo9I GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
LmnI GCTCC 3 cut(s) 345, 860, 1066
Lsp1109I GCAGC 1 cut(s) 1047
LweI GCATC 1 cut(s) 696
MaeII ACGT 3 cut(s) 867, 912, 987
MaeIII GTNAC 1 cut(s) 962
MalI GATC 6 cut(s) 274, 450, 464, 575, 584, 1112
MbiI CCGCTC 1 cut(s) 682
MboI GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
MboII GAAGA 6 cut(s) 13, 66, 391, 418, 592, 1088
MfeI CAATTG 1 cut(s) 374
MflI RGATCY 2 cut(s) 573, 582
MhlI GDGCHC 1 cut(s) 865
MluCI AATT 6 cut(s) 374, 564, 757, 798, 834, 873
MlyI GAGTC 5 cut(s) 305, 436, 491, 741, 1007
MmeI TCCRAC 3 cut(s) 339, 518, 807
MseI TTAA 2 cut(s) 804, 1080
MslI CAYNNNNRTG 2 cut(s) 911, 1052
MspA1I CMGCKG 2 cut(s) 594, 1038
MspI CCGG 2 cut(s) 571, 937
MspR9I CCNGG 4 cut(s) 184, 333, 458, 938
MunI CAATTG 1 cut(s) 374
MvaI CCWGG 3 cut(s) 184, 333, 458
MvnI CGCG 2 cut(s) 550, 652
MwoI GCNNNNNNNGC 4 cut(s) 414, 869, 925, 1032
NciI CCSGG 1 cut(s) 938
NdeII GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
NlaIII CATG 2 cut(s) 491, 910
NlaIV GGNNCC 3 cut(s) 575, 661, 1043
NmeAIII GCCGAG 2 cut(s) 42, 338
NmuCI GTSAC 1 cut(s) 962
OliI CACNNNNGTG 1 cut(s) 1052
PagI TCATGA 2 cut(s) 487, 906
PaqCI CACCTGC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 1119
PfeI GAWTC 5 cut(s) 347, 365, 519, 813, 1008
PflMI CCANNNNNTGG 1 cut(s) 183
PkrI GCNGC 3 cut(s) 649, 1004, 1037
PleI GAGTC 5 cut(s) 305, 436, 491, 741, 1007
PmaCI CACGTG 1 cut(s) 868
PmlI CACGTG 1 cut(s) 868
PpsI GAGTC 5 cut(s) 305, 436, 491, 741, 1007
Ppu21I YACGTR 1 cut(s) 868
Psp6I CCWGG 3 cut(s) 182, 331, 456
PspCI CACGTG 1 cut(s) 868
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 182, 331, 456
PspN4I GGNNCC 3 cut(s) 575, 661, 1043
PspPI GGNCC 3 cut(s) 186, 540, 1042
PstI CTGCAG 1 cut(s) 788
PsuI RGATCY 2 cut(s) 573, 582
RsaI GTAC 2 cut(s) 623, 782
RsaNI GTAC 2 cut(s) 622, 781
RseI CAYNNNNRTG 2 cut(s) 911, 1052
SaqAI TTAA 2 cut(s) 804, 1080
SatI GCNGC 3 cut(s) 648, 1003, 1036
Sau3AI GATC 6 cut(s) 272, 448, 462, 573, 582, 1110
Sau96I GGNCC 3 cut(s) 186, 540, 1042
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 5 cut(s) 305, 436, 491, 741, 1007
ScrFI CCNGG 4 cut(s) 184, 333, 458, 938
SduI GDGCHC 1 cut(s) 865
SfaNI GCATC 1 cut(s) 696
SfcI CTRYAG 1 cut(s) 784
SinI GGWCC 3 cut(s) 186, 540, 1042
SmiMI CAYNNNNRTG 2 cut(s) 911, 1052
Sse9I AATT 6 cut(s) 374, 564, 757, 798, 834, 873
StyD4I CCNGG 4 cut(s) 182, 331, 456, 936
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 2 cut(s) 664, 1123
TaiI ACGT 3 cut(s) 870, 915, 990
TaqI TCGA 7 cut(s) 271, 447, 608, 615, 848, 888, 1011
TaqII GACCGA 1 cut(s) 557
TasI AATT 6 cut(s) 374, 564, 757, 798, 834, 873
TatI WGTACW 2 cut(s) 621, 780
TauI GCSGC 2 cut(s) 650, 1005
TfiI GAWTC 5 cut(s) 347, 365, 519, 813, 1008
Tru1I TTAA 2 cut(s) 804, 1080
Tru9I TTAA 2 cut(s) 804, 1080
TscAI CASTG 4 cut(s) 91, 725, 793, 973
TseFI GTSAC 1 cut(s) 962
TseI GCWGC 1 cut(s) 1035
Tsp45I GTSAC 1 cut(s) 962
TspDTI ATGAA 3 cut(s) 476, 895, 923
TspGWI ACGGA 2 cut(s) 507, 846
TspRI CASTG 4 cut(s) 91, 725, 793, 973
Van91I CCANNNNNTGG 1 cut(s) 183
VpaK11BI GGWCC 3 cut(s) 186, 540, 1042
XapI RAATTY 3 cut(s) 564, 757, 834
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.