Rw5G042080

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013422
Physical Location & Seq
Forward (+)
73792121 .. 73794205
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042080.1

Sequence Viewer

Length: 1101 bp
ATGGCGACCATATCATCATTCTTCAACCGGTTCCTCTCTTTCTTCCTCCTTCTCTTCCACCTCAGCTGCTTCACTATCACCCCCACCGCCGCTGCCTCCACCACTAATCCCCACCGCCATAAACGCAGACTCACTTCATTCCTCTCCAAACCCTCTCTAAAACCCCAAAAGGCTCTTTCCTCCTCTTGGTCCTTTCTCAAGAAAGTCTTCAAGTCCTCTAAAACCCAAACCCACCACGTCTCCACGCCGCCTCGCTCCTCCAGCCACTCCCTCGTCTCCCTGGTCCTCCCAGCCCAAATCAAATCAGACCCACCGCCCAAAAACTCCGATGACTCTGATATCTTCTCCGGCGACCACCACTTTTTCCCTCTCCGAAACGACATCTTCCCCTGCACAACTTGCGGCGAGATTTTCCATAAACCTCAGGCGCTCGACCACCACCAAGCAGTCAAGCACGCCGTGTCGGAGCTCCACAACGGCGACACCGGTAAGAACATAGTCCAGATCATATTCAAAACAGGCTGGACCGACACCCAGAAAAGCCCAGTGATCCACCGCATTCTAAAGATCCACAACTGCGTCAAGATACTTGCCAGGTTCGAAGAGTACAGAGAGTTAGTGAAGTCCAAAGCAGCGAGAAACAGCGCCGTTGGAAGAGACGAAAGGTGCGCAGCTGATGGGAACGAGCTGCTGAGATTTCACTGCTCGACTTTTCTCTGCGACTTGGGAGTCGGCGGGAGCTCCGGGATTTGCAACCAGCAGTTTTGCAGCGTTTGCGGGATCATCAAGTCAGGGTTCTCACCCAAGTTGGACGGAATCTCCACTCTGTCGAGCAGCTCCAGGGCACACATGGCCATCCCGGAGGAGATCGAGGAGGAGTTCAAGTTCATGAACGTGAAGCGGGCCATGCTGGTCTGCCGCGTCGTGGCGGGTCGGGTCGGGTGCGACTCTGATGATGACGTGGACAAGGAGGGTGGCGGGTTTGACTCGGTGGTGGGGAGGAGTGGGAGTGGGACCCAGATGAGGGTGGATGAGGAGGAGCTCTTGGTGTTTAACCCGAGGGCTGTGCTTCCCTGCTTTGTGATTGTGTATACCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

40.5

Weight (kDa)

8.84

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29660
fragaria_vesca FvH4_3g38290 FvH4_3g38290
malus_domestica MD03G1073300.v1.1 MD11G1079200.v1.1
prunus_persica Prupe.6G060000_v2.0.a1
pyrus_communis pycom03g05930 pycom11g06650
rosa_chinensis RchiOBHm_Chr5g0068821
rosa_laevigata RLG00000036012
rosa_multiflora Rmu_sc0014317.1_g000001
rosa_roxburghii Rroxscaffold_1G00012020
rosa_rugosa Rorug05G0393400
rosa_samantha Rh5AG450800 Rh5BG469300 Rh5CG491500 Rh5DG482600
rosa_wichuraiana Rw5G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 728
Acc16I TGCGCA 1 cut(s) 670
AccI GTMKAC 1 cut(s) 1091
AccII CGCG 1 cut(s) 921
AclWI GGATC 3 cut(s) 544, 562, 788
AcoI YGGCCR 1 cut(s) 852
AdeI CACNNNGTG 1 cut(s) 460
AfaI GTAC 1 cut(s) 608
AfiI CCNNNNNNNGG 4 cut(s) 186, 925, 1023, 1024
AgeI ACCGGT 2 cut(s) 27, 485
AgsI TTSAA 4 cut(s) 25, 211, 514, 883
AjiI CACGTC 2 cut(s) 238, 961
AjnI CCWGG 3 cut(s) 279, 593, 839
AloI GAACNNNNNNTCC 2 cut(s) 869, 901
AluBI AGCT 7 cut(s) 66, 469, 674, 688, 741, 837, 1042
AluI AGCT 7 cut(s) 66, 469, 674, 688, 741, 837, 1042
Alw21I GWGCWC 3 cut(s) 471, 743, 1044
Alw26I GTCTC 3 cut(s) 244, 280, 651
AlwI GGATC 3 cut(s) 544, 562, 788
Ama87I CYCGRG 1 cut(s) 1057
AoxI GGCC 2 cut(s) 852, 903
ApeKI GCWGC 7 cut(s) 66, 92, 632, 671, 688, 768, 834
AsiGI ACCGGT 2 cut(s) 27, 485
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 3 cut(s) 430, 647, 671
AspS9I GGNCC 5 cut(s) 189, 283, 525, 903, 1014
AsuC2I CCSGG 2 cut(s) 745, 860
AsuHPI GGTGA 2 cut(s) 70, 792
AsuII TTCGAA 1 cut(s) 600
AvaI CYCGRG 1 cut(s) 1057
AvaII GGWCC 4 cut(s) 189, 283, 525, 1014
AxyI CCTNAGG 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 847
BalI TGGCCA 1 cut(s) 854
BanII GRGCYC 3 cut(s) 471, 743, 1044
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 3 cut(s) 471, 743, 1044
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 7 cut(s) 53, 79, 644, 675, 683, 780, 846
BccI CCATC 2 cut(s) 671, 863
BceAI ACGGC 3 cut(s) 443, 493, 632
BcgI CGANNNNNNTGC 2 cut(s) 1048, 1082
BciT130I CCWGG 3 cut(s) 281, 595, 841
BcnI CCSGG 2 cut(s) 745, 860
BcoDI GTCTC 3 cut(s) 244, 280, 651
BfoI RGCGCY 2 cut(s) 431, 648
Bme1390I CCNGG 5 cut(s) 281, 595, 745, 841, 860
Bme18I GGWCC 4 cut(s) 189, 283, 525, 1014
BmeT110I CYCGRG 1 cut(s) 1057
BmgBI CACGTC 2 cut(s) 238, 961
BmgT120I GGNCC 5 cut(s) 189, 283, 525, 903, 1014
BmiI GGNNCC 3 cut(s) 32, 1015, 1016
BmrFI CCNGG 5 cut(s) 281, 595, 745, 841, 860
BmrI ACTGGG 1 cut(s) 539
BmuI ACTGGG 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 2 cut(s) 244, 823
Bpu10I CCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 2 cut(s) 745, 860
BsaJI CCNNGG 3 cut(s) 279, 840, 1058
BsaWI WCCGGW 2 cut(s) 27, 485
BsaXI ACNNNNNCTCC 8 cut(s) 224, 254, 803, 833, 869, 899, 994, 1024
Bsc4I CCNNNNNNNGG 4 cut(s) 186, 925, 1023, 1024
Bse118I RCCGGY 2 cut(s) 27, 485
Bse1I ACTGG 1 cut(s) 545
Bse21I CCTNAGG 1 cut(s) 423
BseBI CCWGG 3 cut(s) 281, 595, 841
BseDI CCNNGG 3 cut(s) 279, 840, 1058
BseGI GGATG 2 cut(s) 855, 1036
BseLI CCNNNNNNNGG 4 cut(s) 186, 925, 1023, 1024
BseMII CTCAG 3 cut(s) 76, 437, 683
BseNI ACTGG 1 cut(s) 545
BseRI GAGGAG 8 cut(s) 172, 247, 878, 887, 890, 1015, 1049, 1052
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 7 cut(s) 53, 79, 644, 675, 683, 780, 846
BseYI CCCAGC 1 cut(s) 289
BsgI GTGCAG 1 cut(s) 376
Bsh1236I CGCG 1 cut(s) 921
BshFI GGCC 2 cut(s) 854, 905
BshTI ACCGGT 2 cut(s) 27, 485
BsiHKAI GWGCWC 3 cut(s) 471, 743, 1044
BsiHKCI CYCGRG 1 cut(s) 1057
BsiSI CCGG 5 cut(s) 28, 348, 486, 744, 860
BslFI GGGAC 1 cut(s) 1027
BslI CCNNNNNNNGG 4 cut(s) 186, 925, 1023, 1024
BsmAI GTCTC 3 cut(s) 244, 280, 651
BsmBI CGTCTC 3 cut(s) 244, 280, 651
BsmFI GGGAC 1 cut(s) 1027
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 2 cut(s) 854, 905
BsoBI CYCGRG 1 cut(s) 1057
Bsp119I TTCGAA 1 cut(s) 600
Bsp1286I GDGCHC 4 cut(s) 471, 743, 847, 1044
Bsp143I GATC 5 cut(s) 504, 549, 567, 780, 867
BspANI GGCC 2 cut(s) 854, 905
BspCNI CTCAG 3 cut(s) 75, 436, 684
BspFNI CGCG 1 cut(s) 921
BspHI TCATGA 1 cut(s) 888
BspLI GGNNCC 3 cut(s) 32, 1015, 1016
BspPI GGATC 3 cut(s) 544, 562, 788
BspT104I TTCGAA 1 cut(s) 600
BsrFI RCCGGY 2 cut(s) 27, 485
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 2 cut(s) 27, 485
BssECI CCNNGG 3 cut(s) 279, 840, 1058
BssMI GATC 5 cut(s) 504, 549, 567, 780, 867
BssNAI GTATAC 1 cut(s) 1092
Bst1107I GTATAC 1 cut(s) 1092
Bst2UI CCWGG 3 cut(s) 281, 595, 841
Bst4CI ACNGT 1 cut(s) 1096
Bst6I CTCTTC 3 cut(s) 59, 597, 649
BstAPI GCANNNNNTGC 2 cut(s) 399, 774
BstBI TTCGAA 1 cut(s) 600
BstC8I GCNNGC 2 cut(s) 456, 903
BstDEI CTNAG 3 cut(s) 62, 423, 692
BstF5I GGATG 2 cut(s) 855, 1036
BstFNI CGCG 1 cut(s) 921
BstH2I RGCGCY 2 cut(s) 431, 648
BstHHI GCGC 3 cut(s) 430, 647, 671
BstKTI GATC 5 cut(s) 507, 552, 570, 783, 870
BstMAI GTCTC 3 cut(s) 244, 280, 651
BstMBI GATC 5 cut(s) 504, 549, 567, 780, 867
BstMWI GCNNNNNNNGC 6 cut(s) 123, 261, 399, 774, 851, 907
BstNI CCWGG 3 cut(s) 281, 595, 841
BstSCI CCNGG 5 cut(s) 279, 593, 743, 839, 858
BstSLI GKGCMC 1 cut(s) 847
BstUI CGCG 1 cut(s) 921
BstV1I GCAGC 7 cut(s) 53, 79, 644, 675, 683, 780, 846
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BstZ17I GTATAC 1 cut(s) 1092
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 2 cut(s) 854, 905
BtrI CACGTC 2 cut(s) 238, 961
BtsCI GGATG 2 cut(s) 855, 1036
BtsI GCAGTG 1 cut(s) 700
BtsIMutI CAGTG 2 cut(s) 552, 700
Cac8I GCNNGC 2 cut(s) 456, 903
CciI TCATGA 1 cut(s) 888
CfoI GCGC 3 cut(s) 430, 647, 671
Cfr10I RCCGGY 2 cut(s) 27, 485
Cfr13I GGNCC 5 cut(s) 189, 283, 525, 903, 1014
CseI GACGC 2 cut(s) 568, 910
Csp6I GTAC 1 cut(s) 607
CspAI ACCGGT 2 cut(s) 27, 485
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviAII CATG 3 cut(s) 850, 889, 907
CviQI GTAC 1 cut(s) 607
DdeI CTNAG 3 cut(s) 62, 423, 692
DpnI GATC 5 cut(s) 506, 551, 569, 782, 869
DpnII GATC 5 cut(s) 504, 549, 567, 780, 867
DraIII CACNNNGTG 1 cut(s) 460
DrdI GACNNNNNNGTC 1 cut(s) 728
DseDI GACNNNNNNGTC 1 cut(s) 728
EaeI YGGCCR 1 cut(s) 852
Eam1104I CTCTTC 3 cut(s) 59, 597, 649
EarI CTCTTC 3 cut(s) 59, 597, 649
Ecl136II GAGCTC 3 cut(s) 469, 741, 1042
Eco24I GRGCYC 3 cut(s) 471, 743, 1044
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 4 cut(s) 189, 283, 525, 1014
Eco53kI GAGCTC 3 cut(s) 469, 741, 1042
Eco81I CCTNAGG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 1057
EcoICRI GAGCTC 3 cut(s) 469, 741, 1042
EcoO109I RGGNCCY 1 cut(s) 1014
EcoRII CCWGG 3 cut(s) 279, 593, 839
EcoRV GATATC 1 cut(s) 340
EcoT38I GRGCYC 3 cut(s) 471, 743, 1044
Esp3I CGTCTC 3 cut(s) 244, 280, 651
FaeI CATG 3 cut(s) 853, 892, 910
FaiI YATR 9 cut(s) 11, 120, 417, 497, 509, 851, 890, 908, 1092
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 1 cut(s) 1027
FatI CATG 3 cut(s) 849, 888, 906
FauI CCCGC 5 cut(s) 728, 770, 894, 922, 971
FblI GTMKAC 1 cut(s) 1091
FokI GGATG 2 cut(s) 842, 1043
FriOI GRGCYC 3 cut(s) 471, 743, 1044
FspI TGCGCA 1 cut(s) 670
GlaI GCGC 3 cut(s) 429, 646, 670
GsaI CCCAGC 1 cut(s) 293
GsuI CTGGAG 2 cut(s) 244, 823
HaeII RGCGCY 2 cut(s) 431, 648
HaeIII GGCC 2 cut(s) 854, 905
HapII CCGG 5 cut(s) 28, 348, 486, 744, 860
HgaI GACGC 2 cut(s) 568, 910
HhaI GCGC 3 cut(s) 430, 647, 671
Hin1II CATG 3 cut(s) 853, 892, 910
Hin6I GCGC 3 cut(s) 428, 645, 669
HinP1I GCGC 3 cut(s) 428, 645, 669
HinfI GANTC 6 cut(s) 129, 332, 729, 816, 947, 986
HpaII CCGG 5 cut(s) 28, 348, 486, 744, 860
HphI GGTGA 2 cut(s) 70, 792
Hpy166II GTNNAC 2 cut(s) 964, 1092
Hpy188I TCNGA 6 cut(s) 307, 328, 337, 374, 466, 952
Hpy188III TCNNGA 4 cut(s) 199, 502, 583, 889
Hpy8I GTNNAC 2 cut(s) 964, 1092
Hpy99I CGWCG 1 cut(s) 926
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 1 cut(s) 1096
HpyCH4IV ACGT 3 cut(s) 237, 894, 960
HpyCH4V TGCA 3 cut(s) 393, 753, 768
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 261, 399, 774, 851, 907
HpyF3I CTNAG 3 cut(s) 62, 423, 692
HpySE526I ACGT 3 cut(s) 237, 894, 960
Hsp92II CATG 3 cut(s) 853, 892, 910
HspAI GCGC 3 cut(s) 428, 645, 669
KflI GGGWCCC 1 cut(s) 1014
Kzo9I GATC 5 cut(s) 504, 549, 567, 780, 867
LmnI GCTCC 7 cut(s) 260, 466, 474, 738, 746, 842, 1039
Lsp1109I GCAGC 7 cut(s) 53, 79, 644, 675, 683, 780, 846
MaeII ACGT 3 cut(s) 237, 894, 960
MalI GATC 5 cut(s) 506, 551, 569, 782, 869
MboI GATC 5 cut(s) 504, 549, 567, 780, 867
MboII GAAGA 8 cut(s) 13, 34, 46, 199, 334, 376, 614, 666
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 4 cut(s) 471, 743, 847, 1044
MlsI TGGCCA 1 cut(s) 854
MluNI TGGCCA 1 cut(s) 854
MlyI GAGTC 5 cut(s) 123, 326, 738, 941, 980
MmeI TCCRAC 3 cut(s) 444, 631, 789
Mox20I TGGCCA 1 cut(s) 854
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 854
MseI TTAA 1 cut(s) 1053
MslI CAYNNNNRTG 1 cut(s) 893
Msp20I TGGCCA 1 cut(s) 854
MspA1I CMGCKG 3 cut(s) 66, 92, 674
MspI CCGG 5 cut(s) 28, 348, 486, 744, 860
MspR9I CCNGG 5 cut(s) 281, 595, 745, 841, 860
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 3 cut(s) 281, 595, 841
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 6 cut(s) 123, 261, 399, 774, 851, 907
NciI CCSGG 2 cut(s) 745, 860
NdeII GATC 5 cut(s) 504, 549, 567, 780, 867
NlaIII CATG 3 cut(s) 853, 892, 910
NlaIV GGNNCC 3 cut(s) 32, 1015, 1016
NsbI TGCGCA 1 cut(s) 670
NspV TTCGAA 1 cut(s) 600
PagI TCATGA 1 cut(s) 888
PctI GAATGC 1 cut(s) 558
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 816
PfoI TCCNGGA 2 cut(s) 743, 858
PinAI ACCGGT 2 cut(s) 27, 485
PleI GAGTC 5 cut(s) 123, 326, 737, 941, 980
PpsI GAGTC 5 cut(s) 123, 326, 737, 941, 980
PpuMI RGGWCCY 1 cut(s) 1014
Psp124BI GAGCTC 3 cut(s) 471, 743, 1044
Psp5II RGGWCCY 1 cut(s) 1014
Psp6I CCWGG 3 cut(s) 279, 593, 839
PspFI CCCAGC 1 cut(s) 289
PspGI CCWGG 3 cut(s) 279, 593, 839
PspN4I GGNNCC 3 cut(s) 32, 1015, 1016
PspPI GGNCC 5 cut(s) 189, 283, 525, 903, 1014
PspPPI RGGWCCY 1 cut(s) 1014
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 2 cut(s) 66, 674
RsaI GTAC 1 cut(s) 608
RsaNI GTAC 1 cut(s) 607
RseI CAYNNNNRTG 1 cut(s) 893
SacI GAGCTC 3 cut(s) 471, 743, 1044
SaqAI TTAA 1 cut(s) 1053
Sau3AI GATC 5 cut(s) 504, 549, 567, 780, 867
Sau96I GGNCC 5 cut(s) 189, 283, 525, 903, 1014
SchI GAGTC 5 cut(s) 123, 326, 738, 941, 980
ScrFI CCNGG 5 cut(s) 281, 595, 745, 841, 860
SduI GDGCHC 4 cut(s) 471, 743, 847, 1044
SfuI TTCGAA 1 cut(s) 600
SinI GGWCC 4 cut(s) 189, 283, 525, 1014
SmiMI CAYNNNNRTG 1 cut(s) 893
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SstI GAGCTC 3 cut(s) 471, 743, 1044
StyD4I CCNGG 5 cut(s) 279, 593, 743, 839, 858
TaaI ACNGT 1 cut(s) 1096
TaiI ACGT 3 cut(s) 240, 897, 963
TaqI TCGA 5 cut(s) 432, 600, 707, 830, 870
TaqII GACCGA 1 cut(s) 542
TatI WGTACW 1 cut(s) 606
TauI GCSGC 4 cut(s) 92, 250, 405, 921
TfiI GAWTC 1 cut(s) 816
Tru1I TTAA 1 cut(s) 1053
Tru9I TTAA 1 cut(s) 1053
TscAI CASTG 2 cut(s) 552, 707
TseI GCWGC 7 cut(s) 66, 92, 632, 671, 688, 768, 834
TspDTI ATGAA 3 cut(s) 126, 877, 905
TspGWI ACGGA 1 cut(s) 828
TspRI CASTG 2 cut(s) 552, 707
VpaK11BI GGWCC 4 cut(s) 189, 283, 525, 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 1091
XmnI GAANNNNTTC 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.