pycom03g05930

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013422
Physical Location & Seq
Reverse (-)
4697520 .. 4698842
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05930.1

Sequence Viewer

Length: 1323 bp
ATGTCCCACCCCCTCCCCCTCTCTATCTCCTTCACCCTTCATGATCATCTATTCCTTCTCTCTCCTCCTCCTCCTTCTTCCCGAACCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCATGCATATCCTATAGCTTTCTAGACTACATCTACTGTGCTCTCATTTTCCAAAACCCTAATATGGCTAACATATCAACCCTCTTCAACAAGTTCCTCTCTCTCATCATCCTCCTCCTCCACCTCGGCTGCTTCTCTTCCACCGCCACCGCCAATATCCCAAAAAAGCCTCATCACCGTCGTAAACTCTCCACTCACCTCTCCAAACCCAGCACCCTAAAACCCCAAAAAGCCCTCTCCACCTCCTGGTCCTATCTCAAACGCATTTTCACCTCCAAATCCTACAAAATCACCTGCACCAACATCATCCAATCCCACCTCTCCACGCCACCTCGCTCCTCCCACCACTCCATCGTCTCCCTCGTCCTACCCGACTCCGACCCCAAATACCTACCTGGGTCGCTCCCCGAATCAGATATCTCGGCCGATTCCCACCAATGTTTCCCTCTCCGAAACGATATCTTCCCCTGCACCGCCTGCGGGGAAATCTTCCCGAAACCCGAGACTCTCGATCATCACCAGGCGATCCACCACGCCGTTTCGGAGCTTCATGACGGAGACTCGGGCAAAAACATCGTCCGAATCATATTCAAAACAGGTTGGACAGATACAGGAAAAGCCCCCGAAATTCACCGGATCCTGAAGATCCACAACAGCGGCAAAATCCTGGCGAGATTCGAGGAATACAGAGAGCTGGTCAAGTCCAAGGCGGCGCGAAATGGCACCGTTCGGAGGAGGGATGAGCGGTGTATCGCCGACGGCAACGAGCTTCTCAGATTTCACTGCTCAACTTTCGTTTGCGATTTGGGACTCAACGGGAATTCTGGGATTTGTAATCACGAGCACTGCAGTGTTTGTGGAATTATTAAATCTGGATTCTCACCCAAGTTGGACGGAATTTCCACGCTGTCGAGTAGCTCCAGAGCACACGTGGCAATTCCAGAGGATATCGAGGAGGAGTTTCAGTTCATGAACGTGAAGCGGGCTATGCTGGTCTGCCGGGTCGTGGCGGGTCGGGTCGGGTGTGATACTGTGGAGGATATTGACGACGTGGACAAAGAGGGCGGCGGATTCGACTCGGTTGTCGGCAGAGGAGGCAGCGGGGTCCACACGAGGGTGGACGAGGAGGAGCTTTTGGTGTTTAATCCAAGAGCTGTTCTTCCTTGCTTTGTGATCGATATATACCATGTAACGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

48.72

Weight (kDa)

7.9

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29660
fragaria_vesca FvH4_3g38290 FvH4_3g38290
malus_domestica MD03G1073300.v1.1 MD11G1079200.v1.1
prunus_persica Prupe.6G060000_v2.0.a1
pyrus_communis pycom03g05930 pycom11g06650
rosa_chinensis RchiOBHm_Chr5g0068821
rosa_laevigata RLG00000036012
rosa_multiflora Rmu_sc0014317.1_g000001
rosa_roxburghii Rroxscaffold_1G00012020
rosa_rugosa Rorug05G0393400
rosa_samantha Rh5AG450800 Rh5BG469300 Rh5CG491500 Rh5DG482600
rosa_wichuraiana Rw5G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 425
AasI GACNNNNNNGTC 1 cut(s) 1205
Acc36I ACCTGC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 369
AccBSI CCGCTC 1 cut(s) 868
AccII CGCG 1 cut(s) 838
AclWI GGATC 4 cut(s) 643, 754, 763, 767
AcoI YGGCCR 1 cut(s) 546
AcsI RAATTY 3 cut(s) 750, 943, 1020
AcuI CTGAAG 1 cut(s) 785
AcvI CACGTG 1 cut(s) 1054
AfiI CCNNNNNNNGG 7 cut(s) 187, 369, 603, 855, 1129, 1237, 1315
AflIII ACRYGT 1 cut(s) 1051
AgsI TTSAA 2 cut(s) 211, 715
AjiI CACGTC 1 cut(s) 1174
AjnI CCWGG 4 cut(s) 368, 517, 642, 789
AleI CACNNNNGTG 2 cut(s) 972, 1238
AloI GAACNNNNNNTCC 2 cut(s) 1073, 1105
AluBI AGCT 7 cut(s) 141, 670, 817, 892, 1041, 1255, 1277
AluI AGCT 7 cut(s) 141, 670, 817, 892, 1041, 1255, 1277
Alw21I GWGCWC 3 cut(s) 166, 969, 1051
Alw26I GTCTC 3 cut(s) 484, 620, 675
AlwI GGATC 4 cut(s) 643, 754, 763, 767
Ama87I CYCGRG 2 cut(s) 623, 685
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 2 cut(s) 252, 1221
ApoI RAATTY 3 cut(s) 750, 943, 1020
ArsI GACNNNNNNTTYG 2 cut(s) 684, 716
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 2 cut(s) 372, 1228
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 8 cut(s) 25, 290, 311, 385, 406, 632, 746, 996
AvaI CYCGRG 2 cut(s) 623, 685
AvaII GGWCC 2 cut(s) 372, 1228
BamHI GGATCC 1 cut(s) 759
BanI GGYRCC 1 cut(s) 845
BauI CACGAG 2 cut(s) 962, 1234
BbrPI CACGTG 1 cut(s) 1054
Bbv12I GWGCWC 3 cut(s) 166, 969, 1051
BbvI GCAGC 2 cut(s) 239, 1233
BccI CCATC 2 cut(s) 95, 482
BceAI ACGGC 2 cut(s) 644, 898
BcgI CGANNNNNNTGC 2 cut(s) 679, 713
BciT130I CCWGG 4 cut(s) 370, 519, 644, 791
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 3 cut(s) 484, 620, 675
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 2 cut(s) 136, 970
BfuAI ACCTGC 1 cut(s) 425
BisI GCNGC 5 cut(s) 253, 781, 834, 1189, 1222
BlsI GCNGC 5 cut(s) 254, 782, 835, 1190, 1223
Bme1390I CCNGG 5 cut(s) 370, 519, 644, 791, 1124
Bme18I GGWCC 2 cut(s) 372, 1228
BmeT110I CYCGRG 2 cut(s) 623, 685
BmgBI CACGTC 1 cut(s) 1174
BmgT120I GGNCC 2 cut(s) 372, 1228
BmiI GGNNCC 3 cut(s) 761, 847, 1229
BmrFI CCNGG 5 cut(s) 370, 519, 644, 791, 1124
BpmI CTGGAG 1 cut(s) 1027
BpuMI CCSGG 1 cut(s) 1124
Bsa29I ATCGAT 1 cut(s) 1299
BsaAI YACGTR 1 cut(s) 1054
BsaJI CCNNGG 3 cut(s) 247, 518, 828
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 2 cut(s) 1073, 1103
Bsc4I CCNNNNNNNGG 7 cut(s) 187, 369, 603, 855, 1129, 1237, 1315
BseBI CCWGG 4 cut(s) 370, 519, 644, 791
BseCI ATCGAT 1 cut(s) 1299
BseDI CCNNGG 3 cut(s) 247, 518, 828
BseGI GGATG 3 cut(s) 231, 429, 868
BseLI CCNNNNNNNGG 7 cut(s) 187, 369, 603, 855, 1129, 1237, 1315
BseMII CTCAG 1 cut(s) 910
BseX3I CGGCCG 1 cut(s) 546
BseXI GCAGC 2 cut(s) 239, 1233
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 2 cut(s) 403, 577
Bsh1236I CGCG 1 cut(s) 838
Bsh1285I CGRYCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 548
BshNI GGYRCC 1 cut(s) 845
BshVI ATCGAT 1 cut(s) 1299
BsiEI CGRYCG 1 cut(s) 549
BsiHKAI GWGCWC 3 cut(s) 166, 969, 1051
BsiHKCI CYCGRG 2 cut(s) 623, 685
BsiSI CCGG 2 cut(s) 757, 1123
BslFI GGGAC 1 cut(s) 945
BslI CCNNNNNNNGG 7 cut(s) 187, 369, 603, 855, 1129, 1237, 1315
BsmAI GTCTC 3 cut(s) 484, 620, 675
BsmBI CGTCTC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 945
BsnI GGCC 1 cut(s) 548
BsoBI CYCGRG 2 cut(s) 623, 685
Bsp1286I GDGCHC 3 cut(s) 166, 969, 1051
Bsp143I GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 909
BspDI ATCGAT 1 cut(s) 1299
BspFNI CGCG 1 cut(s) 838
BspHI TCATGA 3 cut(s) 40, 673, 1092
BspLI GGNNCC 3 cut(s) 761, 847, 1229
BspMAI CTGCAG 1 cut(s) 974
BspMI ACCTGC 1 cut(s) 425
BspPI GGATC 4 cut(s) 643, 754, 763, 767
BspT107I GGYRCC 1 cut(s) 845
BsrBI CCGCTC 1 cut(s) 868
BssECI CCNNGG 3 cut(s) 247, 518, 828
BssMI GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
BssSI CACGAG 2 cut(s) 962, 1234
BssT1I CCWWGG 1 cut(s) 828
Bst2BI CACGAG 2 cut(s) 962, 1234
Bst2UI CCWGG 4 cut(s) 370, 519, 644, 791
Bst4CI ACNGT 5 cut(s) 161, 302, 850, 1156, 1318
Bst6I CTCTTC 2 cut(s) 212, 265
BstAPI GCANNNNNTGC 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 1054
BstC8I GCNNGC 2 cut(s) 601, 1107
BstDEI CTNAG 1 cut(s) 896
BstF5I GGATG 3 cut(s) 231, 429, 868
BstFNI CGCG 1 cut(s) 838
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 6 cut(s) 46, 637, 651, 762, 771, 1299
BstMAI GTCTC 3 cut(s) 484, 620, 675
BstMBI GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
BstMCI CGRYCG 1 cut(s) 549
BstMWI GCNNNNNNNGC 4 cut(s) 600, 1055, 1111, 1218
BstNI CCWGG 4 cut(s) 370, 519, 644, 791
BstSCI CCNGG 5 cut(s) 368, 517, 642, 789, 1122
BstSFI CTRYAG 2 cut(s) 136, 970
BstUI CGCG 1 cut(s) 838
BstV1I GCAGC 2 cut(s) 239, 1233
BstX2I RGATCY 2 cut(s) 759, 768
BstYI RGATCY 2 cut(s) 759, 768
BstZI CGGCCG 1 cut(s) 546
Bsu15I ATCGAT 1 cut(s) 1299
BsuRI GGCC 1 cut(s) 548
BsuTUI ATCGAT 1 cut(s) 1299
BtrI CACGTC 1 cut(s) 1174
BtsCI GGATG 3 cut(s) 231, 429, 868
BtsI GCAGTG 3 cut(s) 904, 967, 979
BtsIMutI CAGTG 3 cut(s) 904, 967, 979
BveI ACCTGC 1 cut(s) 425
Cac8I GCNNGC 2 cut(s) 601, 1107
CciI TCATGA 3 cut(s) 40, 673, 1092
CfoI GCGC 1 cut(s) 838
Cfr13I GGNCC 2 cut(s) 372, 1228
ClaI ATCGAT 1 cut(s) 1299
CviAII CATG 5 cut(s) 41, 126, 674, 1093, 1310
DdeI CTNAG 1 cut(s) 896
DpnI GATC 6 cut(s) 45, 636, 650, 761, 770, 1298
DpnII GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
DrdI GACNNNNNNGTC 1 cut(s) 1205
DseDI GACNNNNNNGTC 1 cut(s) 1205
EaeI YGGCCR 1 cut(s) 546
EagI CGGCCG 1 cut(s) 546
Eam1104I CTCTTC 2 cut(s) 212, 265
EarI CTCTTC 2 cut(s) 212, 265
EciI GGCGGA 1 cut(s) 1206
EclXI CGGCCG 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 828
Eco32I GATATC 3 cut(s) 541, 583, 1072
Eco47I GGWCC 2 cut(s) 372, 1228
Eco52I CGGCCG 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 785
Eco72I CACGTG 1 cut(s) 1054
Eco88I CYCGRG 2 cut(s) 623, 685
EcoRI GAATTC 1 cut(s) 943
EcoRII CCWGG 4 cut(s) 368, 517, 642, 789
EcoRV GATATC 3 cut(s) 541, 583, 1072
EcoT14I CCWWGG 1 cut(s) 828
EcoT22I ATGCAT 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 828
Esp3I CGTCTC 1 cut(s) 484
FaeI CATG 5 cut(s) 44, 129, 677, 1096, 1313
FaqI GGGAC 1 cut(s) 945
FatI CATG 5 cut(s) 40, 125, 673, 1092, 1309
FauI CCCGC 4 cut(s) 596, 1098, 1126, 1217
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 5 cut(s) 253, 781, 834, 1189, 1222
FokI GGATG 3 cut(s) 218, 416, 875
Fsp4HI GCNGC 5 cut(s) 253, 781, 834, 1189, 1222
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 837
GluI GCNGC 5 cut(s) 253, 781, 834, 1189, 1222
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 1 cut(s) 1027
HaeIII GGCC 1 cut(s) 548
HapII CCGG 2 cut(s) 757, 1123
HhaI GCGC 1 cut(s) 838
Hin1II CATG 5 cut(s) 44, 129, 677, 1096, 1313
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HpaII CCGG 2 cut(s) 757, 1123
HphI GGTGA 8 cut(s) 25, 290, 311, 385, 406, 632, 746, 996
Hpy166II GTNNAC 4 cut(s) 308, 1177, 1231, 1243
Hpy188I TCNGA 7 cut(s) 502, 538, 575, 667, 704, 855, 899
Hpy8I GTNNAC 4 cut(s) 308, 1177, 1231, 1243
Hpy99I CGWCG 3 cut(s) 306, 884, 1175
HpyAV CCTTC 4 cut(s) 40, 47, 65, 84
HpyCH4III ACNGT 5 cut(s) 161, 302, 850, 1156, 1318
HpyCH4IV ACGT 3 cut(s) 1053, 1098, 1173
HpyCH4V TGCA 4 cut(s) 129, 420, 594, 972
HpyF10VI GCNNNNNNNGC 4 cut(s) 600, 1055, 1111, 1218
HpyF3I CTNAG 1 cut(s) 896
HpySE526I ACGT 3 cut(s) 1053, 1098, 1173
Hsp92II CATG 5 cut(s) 44, 129, 677, 1096, 1313
HspAI GCGC 1 cut(s) 836
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
LmnI GCTCC 5 cut(s) 464, 531, 667, 1046, 1252
Lsp1109I GCAGC 2 cut(s) 239, 1233
MaeI CTAG 1 cut(s) 146
MaeII ACGT 3 cut(s) 1053, 1098, 1173
MaeIII GTNAC 1 cut(s) 1312
MalI GATC 6 cut(s) 45, 636, 650, 761, 770, 1298
MbiI CCGCTC 1 cut(s) 868
MboI GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
MboII GAAGA 7 cut(s) 69, 199, 252, 577, 604, 778, 1274
MflI RGATCY 2 cut(s) 759, 768
MhlI GDGCHC 3 cut(s) 166, 969, 1051
MluCI AATT 5 cut(s) 750, 943, 984, 1020, 1059
MlyI GAGTC 5 cut(s) 491, 622, 677, 927, 1193
MmeI TCCRAC 3 cut(s) 525, 704, 993
Mph1103I ATGCAT 1 cut(s) 131
MseI TTAA 2 cut(s) 990, 1266
MslI CAYNNNNRTG 3 cut(s) 972, 1097, 1238
MspA1I CMGCKG 2 cut(s) 780, 1224
MspI CCGG 2 cut(s) 757, 1123
MspR9I CCNGG 5 cut(s) 370, 519, 644, 791, 1124
MvaI CCWGG 4 cut(s) 370, 519, 644, 791
MvnI CGCG 1 cut(s) 838
MwoI GCNNNNNNNGC 4 cut(s) 600, 1055, 1111, 1218
NciI CCSGG 1 cut(s) 1124
NdeII GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
NlaIII CATG 5 cut(s) 44, 129, 677, 1096, 1313
NlaIV GGNNCC 3 cut(s) 761, 847, 1229
NmeAIII GCCGAG 2 cut(s) 228, 524
NsiI ATGCAT 1 cut(s) 131
OliI CACNNNNGTG 2 cut(s) 972, 1238
PagI TCATGA 3 cut(s) 40, 673, 1092
PaqCI CACCTGC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 2 cut(s) 483, 492
PfeI GAWTC 6 cut(s) 533, 551, 705, 798, 999, 1194
PflMI CCANNNNNTGG 1 cut(s) 369
PkrI GCNGC 5 cut(s) 254, 782, 835, 1190, 1223
PleI GAGTC 5 cut(s) 491, 622, 677, 927, 1193
PmaCI CACGTG 1 cut(s) 1054
PmlI CACGTG 1 cut(s) 1054
PpsI GAGTC 5 cut(s) 491, 622, 677, 927, 1193
Ppu21I YACGTR 1 cut(s) 1054
Psp6I CCWGG 4 cut(s) 368, 517, 642, 789
PspCI CACGTG 1 cut(s) 1054
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 4 cut(s) 368, 517, 642, 789
PspN4I GGNNCC 3 cut(s) 761, 847, 1229
PspPI GGNCC 2 cut(s) 372, 1228
PstI CTGCAG 1 cut(s) 974
PsuI RGATCY 2 cut(s) 759, 768
RseI CAYNNNNRTG 3 cut(s) 972, 1097, 1238
SaqAI TTAA 2 cut(s) 990, 1266
SatI GCNGC 5 cut(s) 253, 781, 834, 1189, 1222
Sau3AI GATC 6 cut(s) 43, 634, 648, 759, 768, 1296
Sau96I GGNCC 2 cut(s) 372, 1228
SchI GAGTC 5 cut(s) 491, 622, 677, 927, 1193
ScrFI CCNGG 5 cut(s) 370, 519, 644, 791, 1124
SduI GDGCHC 3 cut(s) 166, 969, 1051
SfcI CTRYAG 2 cut(s) 136, 970
SinI GGWCC 2 cut(s) 372, 1228
SmiMI CAYNNNNRTG 3 cut(s) 972, 1097, 1238
Sse9I AATT 5 cut(s) 750, 943, 984, 1020, 1059
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 5 cut(s) 368, 517, 642, 789, 1122
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 5 cut(s) 161, 302, 850, 1156, 1318
TaiI ACGT 3 cut(s) 1056, 1101, 1176
TaqI TCGA 6 cut(s) 633, 801, 1034, 1074, 1197, 1299
TasI AATT 5 cut(s) 750, 943, 984, 1020, 1059
TauI GCSGC 3 cut(s) 783, 836, 1191
TfiI GAWTC 6 cut(s) 533, 551, 705, 798, 999, 1194
Tru1I TTAA 2 cut(s) 990, 1266
Tru9I TTAA 2 cut(s) 990, 1266
TscAI CASTG 3 cut(s) 911, 974, 979
TseI GCWGC 2 cut(s) 252, 1221
TspDTI ATGAA 4 cut(s) 29, 662, 1081, 1109
TspGWI ACGGA 2 cut(s) 693, 1032
TspRI CASTG 3 cut(s) 911, 974, 979
Van91I CCANNNNNTGG 1 cut(s) 369
VpaK11BI GGWCC 2 cut(s) 372, 1228
XapI RAATTY 3 cut(s) 750, 943, 1020
XbaI TCTAGA 1 cut(s) 145
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XspI CTAG 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.