Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012193

Physical Location & Seq

Genomic Coordinates Reverse (-)

9099002 .. 9104349

Gene Structure 5348 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1106200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4245 bp

ATGGCAATTGAGAAGAACAACTTCAAGGTTTCGAGGCTTGAATCGGAGTTTTCTCCCAGTAGTAGGAAGAGTTTGTCTAGTGATGAGGATGAGCTACAACAGCGTAGCTCTGCTGTGGAATCCGACGACGACGATGAATTTGATGACGCAGACTCGGGGGCGGGGTCTGATGACTTTGATTTGTTGGAATTGGGGGAAACCGGTGTGGAGTTTTGCCAAGTTGGGAATCAGACTTGTAGCATTCCTTTCGAGCTGTATGATCTTCCAAGCCTTGAAGACATATTGTCAGTGGATGTGTGGAATGAGTGCTTGAGTGAGGAGGAGCAGTTTGGCCTCACTAAGTATCTGCCTGATATGGACCAGGAGAGCTTTATGATTACCTTGAAAGAGCTTTTTACGGGTTGCAACTTTCATTTTGGGAGCCCTGTTAAGCAGTTGTTTGATATGTTGAAGGGAGGTTTGTGCGAGCCAAGAGTTGCTCTTTACCGGGAGGGTTTGAATTTCTTTCAGAAGCGGCAGCACTACAATCTTTTGAGGAAACATCAGAACAATATGGTTAGTAATCTGTGTCAAATAAGGGATGCTTGGCTTAATTGCAAAGGATATAGTATTGAGGAGAGGCTCCGCGTGCTGAATATTATGAGAATCCAGAAGAGTTTGATGGGTGAGAAGATGGAAGATATGGAGGCTGACTCATCAGAAAGAGAGTCGGGTGAAGGTTTACGGAGCAACAAAATCAAAGACAGGAAAACTGCACAGAAAATGGCTCGTTATTCTCCATATGGGTTGGATACAAGTGTGGAACTTGCATCAAGAGGGCCGTCATCGGCTATGGAATTTGCAAAATATGGAAAGCAGAATCCAAAAGGTATACTGAAGTTGGCCGGGTCCAAGGCTCCTTCAGCAAAAGAGCTGGCAAACCATTCAGGACTATACAGTTCAGCAGTAGCTCTTCCTCGGCAGCATAAAGCAGGAGGGTATGATGCTGGGGTGGCATTTAGAATGAGGGATCAATTGATAAGTGGGGATGATGTTGAAGACACAGCATATGGAACTGGCATTCAGCGAGATAGAAATGTGTCTCGTGGTAACTCTATGGATAGGTCTGGTGTTTTCAAAGTGGGAAAGAATCATGACCTTTTAAGAGGTGACGAATTAAACATTGACAGTTTGATGGGTCTGCCTCTTTCCTCAAAGGCTGACATTTATGCCTATGGTAGGAACCACAGTGTAAACCTTTTGTCAGAGGCAAAGGTGTTAACAGCAAAGCCTCCTAATTTAAGAGCTCCCTATGATTTTGTTAAAAAGGCCAAGTATCCAGAAAATATTCATCAATTCACAGCTGGGGATCAGACGAAGTCCTCGAAAGCCAGACTTCTGCAGCCACCTCTCAGAGGAGATCGAGCTGACTTATCAGAACGTGCAGAACCATTCTGGCATAAAAGGACTGAAGGGGAGACTTTTTCTATGGATTCTCCCTTAAGAGCGGATGATTGGAATGCTAGGAGCAAGAAATGGAAGACAGGGAGGGAGTCTCATGATCTAAATTACAAATCATATAGAGCTTCCCCACCACAGATGAATGATAGGTTTATATCTTCTGAATTTAGAGCAAAACCATTGCAAGAGAAGACAAGAGAGAAGAGAATACAAAATGGAGGATCAGAAATGGCAGCATTGAAAGGTAATAGAATGTTTGTTAAAAATGAAGATACAGAATCAGACTCATCGGAGCAATTTGACGACGATGAGGATAGCAACCCGTTGTTGAGGAGCAAGTTGGCTTATCCCAGTGGTGTTATGGAACCTTCCCCATCTTCTTTGTTGAATCCCACTCTAGATGCAAAAAGGGCCAAATACTCGAAGAAAGAGGTGAAGGATAGTTTACAGGCTCTTGATGGGATCAATTACTCCAGTAAGATGGGCGGTTTTGTGGAACATGGACATATGCGTAATCTAGGGAACTATTCTTCCAAAGCAAAACAAAAGGGTAAGATGCGAGACAATAGTCCCTTACATAACTCTTCGACTAGAGCTTTGGAAGAGCGTTATATCCCGGGATTGAGTAAGTTTAAGGATGATGGTGACGATTATGAAGAACAGAAACAAATCTACAAAATGGGCAAGAATGCCCAATTTCAAGGGGAAGCTGGTGAAAGGTTACATACACCCTCATGGAAAGTCTACACTGGAAAGCAGAAAAGAGAAGTTGGCCATCATCATTCTGTGCCAGAGTCTCGTTATTTTGTTGATGAGGAGGATGATTCACATGAAATGCAGTTACTAGGTAATGGTAGTGGACAGGGTAATATCAGGAAGAAAGGTCAGAACTTTGAAGACTGTGACAGTGATCGCCATGAAAGGATTGAGGTCCCATTATTAGGGTGCAACATGGTGGCAAAGAAGCGGAAAGGAAAGGAGGATGTGTTGGATACTGGTAGAGGAGATGAAGATGGTGATCTACAGTCTAACCATTCACAGCTCATTGTTGAGTCCAGTTCCTTGAAAAAAAGGGCAAAGAGGAAGCTAGAGAATGAGAATGTTAGTTCAGATGTAGAAATTTCTGAGCAGCCAATTACAGAAATGGGAGCAACAGAGATGGAGCCGGAAACCAAGCCCCAGAAAAAGGCATTTACTCCAATTACACCTACAGTTCATGCTGGTTTCTCATTCTCGATTATACATCTTCTTTCAGCTGTTCGCTTGGCAATGATCACTCCAGTTCCGGAAGGTACTGTTGGGGAATCTGTCGATGAGCAAAACAAAAATCACGAGGGTGCTGTCAATGGGGTTCTTTCTTGTGAAAAGGTGGATGTCAATAACTCAGAGCTTGCTGGAGAAATGAATATGCCTTCTCTAACGGTTCAGGAGATTGTGAACCGTGTTAGCTCGAATCCAGGAGATCCTTGTATCATTGAGACACAAGAGCCACTTCAGGATCTTGTGAGAGGAGTTCTAAGGATATTTTCATCAAAAACAGCACCTTTAGGAGCAAAGGGTTGGAAGACGCTGGTTGTCTTTGAAAAAGCAACAAAAAGCTGGTTGTGGACCGGGCCAGTTTCTCAGAGTTCATCAGATCGTGACGCTAATGAGGAGGTGATATCTCCTGAAGCATGGGGTCTTCCGCACAAAATGCTTGTCAAGCTGGTGGATTCGTTTGCTAATTGGCTCAAATGCGGGCAAGATACCATTCAACAAATTGGAATTCTTCCGGCACCGCCCTTGGAGTTGATGCAGCTCAACCTGGATGAGAAAGAAAGGTTCAGGGATTTAAGAGCTCAAAAGAGTCTTAACACCATTAACCCAAGTTCTGAAGAAGTCAGGGCTTATTTCCGGAAGGAGGAGGTTCTTAGGTATTCAATTCCAGACAGGGCCTTCTCTTACACAGCAGCTGATGGTAAAAAATCCATTGTTGCTCCATTGAGAAGGTGCGGTGGTAAGCCAACATCAAAAGCCCGAGATCACTTTATGCTGAAACGTGATCGACCACCGCATGTTACAATTCTTTGTCTTGTGAGAGATGCAGCTGCAAGGTTGCCTGGAAGTATTGGCACCAGAGCAGATGTTTGCACTTTGATAAGAGATTCTCAGTACATTGTTGAAGATGTGTCTGATGCACAAGTTAATCAAGTTGTTAGTGGAGCCCTTGATCGGTTGCATTATGAGCGTGATCCTTGTGTGCAATTTGACGGGGAAAGGAAATTATGGGTCTACTTACATAGGGAAAGAGAAGAAGAAGATTTTGAAGATGATGGTACTTCATCTACAAAGAAATGGAAGAGGCAGAAAAAGGATGCTGGCGATCTACCTGACCAAGGAGCAGTGACTGTGGCTTATCATGGGACTGAGGAACAAACTGGATATGATGTGTGCTCCGATCTCAATGTCGAGCCATCATGTTTGGATGATATGCAACAAGATGTGGAGGATAATACTGATACCAATAACGGGTCTGAACAAGATGAAATGCGCCAAGGCGATCCACTGCTTTGGGAGGGTGTTGGCTTAAATCCAACGCATGAAAACAAATTGTTGTGTCAGGAAAATTCCACAAATGAAGATTTTGATGATGAAACGTTTGGGAGAGAAAGAACAGTTGGGATCTTGAGCATAGCACTCCAACCTCCCATCAGATCGCGGATTGCAGATCGCATGGTTGTTCCCAGGTCAAGGAGTGTGTCATACAATGAAGGTGCAGTATTTTTGCATATAATATTGATTCCATCAGGAAAACGTGTAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0002020 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0019899 GO:0031011 GO:0031974 GO:0031981 GO:0032991 GO:0033202 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0070603 GO:0097346 GO:1902494 GO:1904949

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1415

Amino Acids

158.88

Weight (kDa)

5.52

Isoelectric Point (pI)

47.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_2nd_NFRKB	PF25793	1108 - 1246	7.4e-65	NFRKB second winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45830
fragaria_vesca	FvH4_3g34430 FvH4_3g34430
malus_domestica	MD03G1106200.v1.1 MD11G1118300.v1.1
prunus_persica	Prupe.6G088700_v2.0.a1
pyrus_communis	pycom03g08520 pycom11g09930
rosa_chinensis	RchiOBHm_Chr5g0062031
rosa_laevigata	RLG00000035527
rosa_multiflora	Rmu_sc0002296.1_g000008 Rmu_sc0017669.1_g000005
rosa_roxburghii	Rroxscaffold_1G00018660
rosa_rugosa	Rorug05G0345700
rosa_samantha	Rh5AG407000 Rh5BG420300 Rh5CG444400 Rh5DG435700
rosa_wichuraiana	Rw5G038350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	3214, 3551
AccBSI	CCGCTC	1 cut(s)	1487
AccI	GTMKAC	3 cut(s)	871, 2184, 3711
AccII	CGCG	2 cut(s)	627, 4138
AccIII	TCCGGA	2 cut(s)	2725, 3333
AclI	AACGTT	1 cut(s)	4076
AclWI	GGATC	9 cut(s)	1017, 1356, 1669, 1910, 2897, 2946, 3665, 3974, 4109
AcoI	YGGCCR	2 cut(s)	882, 2212
AcsI	RAATTY	7 cut(s)	137, 499, 836, 1604, 2559, 3204, 4045
AcuI	CTGAAG	6 cut(s)	885, 896, 1470, 2918, 3129, 3333
AfaI	GTAC	3 cut(s)	2734, 3593, 3757
AflII	CTTAAG	1 cut(s)	1480
AflIII	ACRYGT	1 cut(s)	4234
AgeI	ACCGGT	1 cut(s)	200
AhdI	GACNNNNNGTC	1 cut(s)	283
AjnI	CCWGG	5 cut(s)	360, 2896, 3243, 3538, 4163
AjuI	GAANNNNNNNTTGG	4 cut(s)	2721, 2753, 4080, 4112
AleI	CACNNNNGTG	1 cut(s)	2775
AloI	GAACNNNNNNTCC	2 cut(s)	1955, 1987
Alw21I	GWGCWC	3 cut(s)	1288, 3280, 3875
Alw26I	GTCTC	6 cut(s)	1086, 1451, 1539, 1995, 2241, 2912
AlwI	GGATC	9 cut(s)	1017, 1356, 1669, 1910, 2897, 2946, 3665, 3974, 4109
AlwNI	CAGNNNCTG	2 cut(s)	3392, 3827
Ama87I	CYCGRG	3 cut(s)	154, 2056, 3456
Aor13HI	TCCGGA	2 cut(s)	2725, 3333
AoxI	GGCC	8 cut(s)	331, 818, 882, 1308, 1851, 2212, 3053, 3372
ApeKI	GCWGC	9 cut(s)	517, 961, 1381, 1673, 2569, 3235, 3389, 3524, 3527
ApoI	RAATTY	7 cut(s)	137, 499, 836, 1604, 2559, 3204, 4045
ArsI	GACNNNNNNTTYG	6 cut(s)	122, 154, 2020, 2052, 3123, 3155
AsiGI	ACCGGT	1 cut(s)	200
Asp700I	GAANNNNTTC	2 cut(s)	20, 1326
AspLEI	GCGC	1 cut(s)	3972
AspS9I	GGNCC	8 cut(s)	358, 818, 888, 1851, 2371, 3048, 3053, 3372
AsuC2I	CCSGG	5 cut(s)	488, 886, 2057, 2058, 3052
AsuHPI	GGTGA	8 cut(s)	677, 725, 1160, 1885, 2096, 2165, 2468, 3109
AvaI	CYCGRG	3 cut(s)	154, 2056, 3456
AvaII	GGWCC	4 cut(s)	358, 888, 2371, 3048
BaeI	ACNNNNGTAYC	2 cut(s)	783, 816
BalI	TGGCCA	1 cut(s)	2214
BanI	GGYRCC	2 cut(s)	3214, 3551
BanII	GRGCYC	4 cut(s)	425, 1288, 3280, 3646
BauI	CACGAG	2 cut(s)	1083, 2771
BbsI	GAAGAC	7 cut(s)	282, 1044, 1526, 1637, 2343, 3011, 3113
Bbv12I	GWGCWC	3 cut(s)	1288, 3280, 3875
BbvI	GCAGC	9 cut(s)	529, 973, 1393, 1685, 2581, 3247, 3401, 3514, 3536
BceAI	ACGGC	1 cut(s)	805
BcgI	CGANNNNNNTGC	2 cut(s)	229, 263
BciT130I	CCWGG	5 cut(s)	362, 2898, 3245, 3540, 4165
BciVI	GTATCC	3 cut(s)	784, 1326, 2425
BclI	TGATCA	1 cut(s)	2712
BcnI	CCSGG	5 cut(s)	488, 886, 2057, 2058, 3052
BcoDI	GTCTC	6 cut(s)	1086, 1451, 1539, 1995, 2241, 2912
BfaI	CTAG	7 cut(s)	78, 1503, 1838, 1958, 2031, 2285, 2528
BfmI	CTRYAG	3 cut(s)	1379, 2462, 2649
BfrI	CTTAAG	1 cut(s)	1480
BfuI	GTATCC	3 cut(s)	784, 1326, 2425
Bme18I	GGWCC	4 cut(s)	358, 888, 2371, 3048
BmeRI	GACNNNNNGTC	1 cut(s)	283
BmeT110I	CYCGRG	3 cut(s)	154, 2056, 3456
BmgT120I	GGNCC	8 cut(s)	358, 818, 888, 1851, 2371, 3048, 3053, 3372
BmrI	ACTGGG	2 cut(s)	51, 1785
BmsI	GCATC	9 cut(s)	571, 818, 973, 1831, 1986, 3222, 3511, 3604, 3784
BmuI	ACTGGG	2 cut(s)	51, 1785
BoxI	GACNNNNGTC	1 cut(s)	2007
BpiI	GAAGAC	7 cut(s)	282, 1044, 1526, 1637, 2343, 3011, 3113
BplI	GAGNNNNNCTC	4 cut(s)	677, 709, 1519, 1551
BpmI	CTGGAG	3 cut(s)	1897, 2703, 2856
BpuEI	CTTGAG	2 cut(s)	331, 4126
BpuMI	CCSGG	5 cut(s)	488, 886, 2057, 2058, 3052
BsaBI	GATNNNNATC	1 cut(s)	2457
BsaJI	CCNNGG	7 cut(s)	891, 956, 2056, 3222, 3814, 3973, 4163
BsaWI	WCCGGW	3 cut(s)	200, 2725, 3333
BsaXI	ACNNNNNCTCC	4 cut(s)	311, 341, 2411, 2441
Bse118I	RCCGGY	1 cut(s)	200
Bse3DI	GCAATG	2 cut(s)	1619, 2715
Bse8I	GATNNNNATC	1 cut(s)	2457
BseAI	TCCGGA	2 cut(s)	2725, 3333
BseBI	CCWGG	5 cut(s)	362, 2898, 3245, 3540, 4165
BseDI	CCNNGG	7 cut(s)	891, 956, 2056, 3222, 3814, 3973, 4163
BseJI	GATNNNNATC	1 cut(s)	2457
BseMI	GCAATG	2 cut(s)	1619, 2715
BseMII	CTCAG	6 cut(s)	1405, 2556, 2838, 3077, 3602, 3837
BseXI	GCAGC	9 cut(s)	529, 973, 1393, 1685, 2581, 3247, 3401, 3514, 3536
BseYI	CCCAGC	2 cut(s)	986, 1343
BsgI	GTGCAG	3 cut(s)	738, 1443, 4215
Bsh1236I	CGCG	2 cut(s)	627, 4138
BshFI	GGCC	8 cut(s)	333, 820, 884, 1310, 1853, 2214, 3055, 3374
BshNI	GGYRCC	2 cut(s)	3214, 3551
BshTI	ACCGGT	1 cut(s)	200
BsiHKAI	GWGCWC	3 cut(s)	1288, 3280, 3875
BsiHKCI	CYCGRG	3 cut(s)	154, 2056, 3456
BsiSI	CCGG	9 cut(s)	201, 487, 885, 2057, 2606, 2726, 3051, 3212, 3334
BslFI	GGGAC	3 cut(s)	1995, 2357, 3856
BsmAI	GTCTC	6 cut(s)	1086, 1451, 1539, 1995, 2241, 2912
BsmFI	GGGAC	3 cut(s)	1995, 2357, 3856
BsmI	GAATGC	4 cut(s)	240, 1059, 1504, 2134
BsnI	GGCC	8 cut(s)	333, 820, 884, 1310, 1853, 2214, 3055, 3374
BsoBI	CYCGRG	3 cut(s)	154, 2056, 3456
Bsp1286I	GDGCHC	5 cut(s)	425, 1288, 3280, 3646, 3875
Bsp13I	TCCGGA	2 cut(s)	2725, 3333
BspANI	GGCC	8 cut(s)	333, 820, 884, 1310, 1853, 2214, 3055, 3374
BspCNI	CTCAG	6 cut(s)	1404, 2557, 2837, 3076, 3601, 3838
BspEI	TCCGGA	2 cut(s)	2725, 3333
BspFNI	CGCG	2 cut(s)	627, 4138
BspHI	TCATGA	2 cut(s)	1132, 1537
BspMAI	CTGCAG	1 cut(s)	1383
BspPI	GGATC	9 cut(s)	1017, 1356, 1669, 1910, 2897, 2946, 3665, 3974, 4109
BspQI	GCTCTTC	2 cut(s)	957, 2037
BspT107I	GGYRCC	2 cut(s)	3214, 3551
BspTI	CTTAAG	1 cut(s)	1480
BsrBI	CCGCTC	1 cut(s)	1487
BsrDI	GCAATG	2 cut(s)	1619, 2715
BsrFI	RCCGGY	1 cut(s)	200
BssAI	RCCGGY	1 cut(s)	200
BssECI	CCNNGG	7 cut(s)	891, 956, 2056, 3222, 3814, 3973, 4163
BssNAI	GTATAC	1 cut(s)	872
BssSI	CACGAG	2 cut(s)	1083, 2771
BssT1I	CCWWGG	4 cut(s)	891, 3222, 3814, 3973
Bst1107I	GTATAC	1 cut(s)	872
Bst2BI	CACGAG	2 cut(s)	1083, 2771
Bst2UI	CCWGG	5 cut(s)	362, 2898, 3245, 3540, 4165
Bst6I	CTCTTC	7 cut(s)	62, 647, 957, 1637, 2029, 2037, 3773
BstAFI	CTTAAG	1 cut(s)	1480
BstAPI	GCANNNNNTGC	1 cut(s)	3133
BstC8I	GCNNGC	6 cut(s)	467, 629, 915, 2832, 3179, 3799
BstDEI	CTNAG	9 cut(s)	339, 1391, 2565, 2824, 2957, 3063, 3350, 3588, 3846
BstENI	CCTNNNNNAGG	3 cut(s)	1216, 1392, 3813
BstFNI	CGCG	2 cut(s)	627, 4138
BstHHI	GCGC	1 cut(s)	3972
BstMAI	GTCTC	6 cut(s)	1086, 1451, 1539, 1995, 2241, 2912
BstMWI	GCNNNNNNNGC	8 cut(s)	100, 628, 902, 992, 1850, 3133, 3142, 3664
BstNI	CCWGG	5 cut(s)	362, 2898, 3245, 3540, 4165
BstNSI	RCATGY	1 cut(s)	3497
BstPAI	GACNNNNGTC	1 cut(s)	2007
BstSFI	CTRYAG	3 cut(s)	1379, 2462, 2649
BstUI	CGCG	2 cut(s)	627, 4138
BstV1I	GCAGC	9 cut(s)	529, 973, 1393, 1685, 2581, 3247, 3401, 3514, 3536
BstV2I	GAAGAC	7 cut(s)	282, 1044, 1526, 1637, 2343, 3011, 3113
BstX2I	RGATCY	3 cut(s)	2902, 2938, 4101
BstXI	CCANNNNNNTGG	2 cut(s)	1921, 3990
BstYI	RGATCY	3 cut(s)	2902, 2938, 4101
BstZ17I	GTATAC	1 cut(s)	872
BsuI	GTATCC	3 cut(s)	784, 1326, 2425
BsuRI	GGCC	8 cut(s)	333, 820, 884, 1310, 1853, 2214, 3055, 3374
BtsI	GCAGTG	2 cut(s)	3828, 3983
BtsIMutI	CAGTG	7 cut(s)	294, 1234, 1798, 2187, 2353, 3828, 3983
Cac8I	GCNNGC	6 cut(s)	467, 629, 915, 2832, 3179, 3799
CaiI	CAGNNNCTG	2 cut(s)	3392, 3827
CciI	TCATGA	2 cut(s)	1132, 1537
CfoI	GCGC	1 cut(s)	3972
Cfr10I	RCCGGY	1 cut(s)	200
Cfr13I	GGNCC	8 cut(s)	358, 818, 888, 1851, 2371, 3048, 3053, 3372
Cfr9I	CCCGGG	1 cut(s)	2056
CseI	GACGC	3 cut(s)	155, 3016, 3092
Csp6I	GTAC	3 cut(s)	2733, 3592, 3756
CspAI	ACCGGT	1 cut(s)	200
CviQI	GTAC	3 cut(s)	2733, 3592, 3756
DdeI	CTNAG	9 cut(s)	339, 1391, 2565, 2824, 2957, 3063, 3350, 3588, 3846
DriI	GACNNNNNGTC	1 cut(s)	283
EaeI	YGGCCR	2 cut(s)	882, 2212
Eam1104I	CTCTTC	7 cut(s)	62, 647, 957, 1637, 2029, 2037, 3773
Eam1105I	GACNNNNNGTC	1 cut(s)	283
EarI	CTCTTC	7 cut(s)	62, 647, 957, 1637, 2029, 2037, 3773
Ecl136II	GAGCTC	2 cut(s)	1286, 3278
Eco130I	CCWWGG	4 cut(s)	891, 3222, 3814, 3973
Eco24I	GRGCYC	4 cut(s)	425, 1288, 3280, 3646
Eco32I	GATATC	1 cut(s)	3102
Eco47I	GGWCC	4 cut(s)	358, 888, 2371, 3048
Eco53kI	GAGCTC	2 cut(s)	1286, 3278
Eco57I	CTGAAG	6 cut(s)	885, 896, 1470, 2918, 3129, 3333
Eco88I	CYCGRG	3 cut(s)	154, 2056, 3456
EcoICRI	GAGCTC	2 cut(s)	1286, 3278
EcoNI	CCTNNNNNAGG	3 cut(s)	1216, 1392, 3813
EcoO109I	RGGNCCY	2 cut(s)	2371, 3372
EcoRI	GAATTC	1 cut(s)	3204
EcoRII	CCWGG	5 cut(s)	360, 2896, 3243, 3538, 4163
EcoRV	GATATC	1 cut(s)	3102
EcoT14I	CCWWGG	4 cut(s)	891, 3222, 3814, 3973
EcoT38I	GRGCYC	4 cut(s)	425, 1288, 3280, 3646
ErhI	CCWWGG	4 cut(s)	891, 3222, 3814, 3973
FalI	AAGNNNNNCTT	7 cut(s)	37, 568, 600, 1359, 1391, 2916, 2948
FaqI	GGGAC	3 cut(s)	1995, 2357, 3856
FauI	CCCGC	2 cut(s)	154, 3170
FauNDI	CATATG	3 cut(s)	781, 1048, 1947
FbaI	TGATCA	1 cut(s)	2712
FblI	GTMKAC	3 cut(s)	871, 2184, 3711
FriOI	GRGCYC	4 cut(s)	425, 1288, 3280, 3646
FspBI	CTAG	7 cut(s)	78, 1503, 1838, 1958, 2031, 2285, 2528
GlaI	GCGC	1 cut(s)	3971
GsaI	CCCAGC	2 cut(s)	990, 1347
GsuI	CTGGAG	3 cut(s)	1897, 2703, 2856
HaeIII	GGCC	8 cut(s)	333, 820, 884, 1310, 1853, 2214, 3055, 3374
HapII	CCGG	9 cut(s)	201, 487, 885, 2057, 2606, 2726, 3051, 3212, 3334
HgaI	GACGC	3 cut(s)	155, 3016, 3092
HhaI	GCGC	1 cut(s)	3972
Hin6I	GCGC	1 cut(s)	3970
HinP1I	GCGC	1 cut(s)	3970
HincII	GTYRAC	1 cut(s)	1260
HindII	GTYRAC	1 cut(s)	1260
HpaI	GTTAAC	1 cut(s)	1260
HpaII	CCGG	9 cut(s)	201, 487, 885, 2057, 2606, 2726, 3051, 3212, 3334
HphI	GGTGA	8 cut(s)	677, 725, 1160, 1885, 2096, 2165, 2468, 3109
Hpy99I	CGWCG	4 cut(s)	128, 131, 134, 1748
HpyCH4IV	ACGT	4 cut(s)	1420, 3478, 4076, 4234
HpyF10VI	GCNNNNNNNGC	8 cut(s)	100, 628, 902, 992, 1850, 3133, 3142, 3664
HpyF3I	CTNAG	9 cut(s)	339, 1391, 2565, 2824, 2957, 3063, 3350, 3588, 3846
HpySE526I	ACGT	4 cut(s)	1420, 3478, 4076, 4234
HspAI	GCGC	1 cut(s)	3970
Kpn2I	TCCGGA	2 cut(s)	2725, 3333
Ksp22I	TGATCA	1 cut(s)	2712
KspAI	GTTAAC	1 cut(s)	1260
LguI	GCTCTTC	2 cut(s)	957, 2037
Lsp1109I	GCAGC	9 cut(s)	529, 973, 1393, 1685, 2581, 3247, 3401, 3514, 3536
LweI	GCATC	9 cut(s)	571, 818, 973, 1831, 1986, 3222, 3511, 3604, 3784
MaeI	CTAG	7 cut(s)	78, 1503, 1838, 1958, 2031, 2285, 2528
MaeII	ACGT	4 cut(s)	1420, 3478, 4076, 4234
MaeIII	GTNAC	9 cut(s)	1088, 1148, 2084, 2160, 2280, 2342, 3080, 3496, 3823
MbiI	CCGCTC	1 cut(s)	1487
MfeI	CAATTG	2 cut(s)	6, 1013
MflI	RGATCY	3 cut(s)	2902, 2938, 4101
MhlI	GDGCHC	5 cut(s)	425, 1288, 3280, 3646, 3875
MlsI	TGGCCA	1 cut(s)	2214
MluNI	TGGCCA	1 cut(s)	2214
MlyI	GAGTC	8 cut(s)	146, 686, 716, 1541, 1718, 2243, 2501, 3295
MmeI	TCCRAC	7 cut(s)	147, 165, 768, 2409, 2981, 4037, 4144
Mox20I	TGGCCA	1 cut(s)	2214
MroI	TCCGGA	2 cut(s)	2725, 3333
MroXI	GAANNNNTTC	2 cut(s)	20, 1326
MscI	TGGCCA	1 cut(s)	2214
MslI	CAYNNNNRTG	2 cut(s)	2173, 2775
Msp20I	TGGCCA	1 cut(s)	2214
MspA1I	CMGCKG	4 cut(s)	1343, 2696, 3392, 3527
MspCI	CTTAAG	1 cut(s)	1480
MspI	CCGG	9 cut(s)	201, 487, 885, 2057, 2606, 2726, 3051, 3212, 3334
MunI	CAATTG	2 cut(s)	6, 1013
Mva1269I	GAATGC	4 cut(s)	240, 1059, 1504, 2134
MvaI	CCWGG	5 cut(s)	362, 2898, 3245, 3540, 4165
MvnI	CGCG	2 cut(s)	627, 4138
MwoI	GCNNNNNNNGC	8 cut(s)	100, 628, 902, 992, 1850, 3133, 3142, 3664
NciI	CCSGG	5 cut(s)	488, 886, 2057, 2058, 3052
NdeI	CATATG	3 cut(s)	781, 1048, 1947
NmeAIII	GCCGAG	1 cut(s)	937
NmuCI	GTSAC	5 cut(s)	1148, 2084, 2342, 3080, 3823
NspI	RCATGY	1 cut(s)	3497
OliI	CACNNNNGTG	1 cut(s)	2775
PagI	TCATGA	2 cut(s)	1132, 1537
PciSI	GCTCTTC	2 cut(s)	957, 2037
PcsI	WCGNNNNNNNCGW	1 cut(s)	1361
PctI	GAATGC	4 cut(s)	240, 1059, 1504, 2134
PdmI	GAANNNNTTC	2 cut(s)	20, 1326
PflFI	GACNNNGTC	1 cut(s)	1357
PfoI	TCCNGGA	1 cut(s)	2896
PinAI	ACCGGT	1 cut(s)	200
PleI	GAGTC	8 cut(s)	146, 686, 715, 1540, 1718, 2242, 2500, 3294
PpsI	GAGTC	8 cut(s)	146, 686, 715, 1540, 1718, 2242, 2500, 3294
PpuMI	RGGWCCY	1 cut(s)	2371
PshAI	GACNNNNGTC	1 cut(s)	2007
Psp124BI	GAGCTC	2 cut(s)	1288, 3280
Psp1406I	AACGTT	1 cut(s)	4076
Psp5II	RGGWCCY	1 cut(s)	2371
Psp6I	CCWGG	5 cut(s)	360, 2896, 3243, 3538, 4163
PspFI	CCCAGC	2 cut(s)	986, 1343
PspGI	CCWGG	5 cut(s)	360, 2896, 3243, 3538, 4163
PspPI	GGNCC	8 cut(s)	358, 818, 888, 1851, 2371, 3048, 3053, 3372
PspPPI	RGGWCCY	1 cut(s)	2371
PstI	CTGCAG	1 cut(s)	1383
PstNI	CAGNNNCTG	2 cut(s)	3392, 3827
PsuI	RGATCY	3 cut(s)	2902, 2938, 4101
PsyI	GACNNNGTC	1 cut(s)	1357
PvuII	CAGCTG	4 cut(s)	1343, 2696, 3392, 3527
RsaI	GTAC	3 cut(s)	2734, 3593, 3757
RsaNI	GTAC	3 cut(s)	2733, 3592, 3756
RseI	CAYNNNNRTG	2 cut(s)	2173, 2775
SacI	GAGCTC	2 cut(s)	1288, 3280
SapI	GCTCTTC	2 cut(s)	957, 2037
Sau96I	GGNCC	8 cut(s)	358, 818, 888, 1851, 2371, 3048, 3053, 3372
SchI	GAGTC	8 cut(s)	146, 686, 716, 1541, 1718, 2243, 2501, 3295
SduI	GDGCHC	5 cut(s)	425, 1288, 3280, 3646, 3875
SfaNI	GCATC	9 cut(s)	571, 818, 973, 1831, 1986, 3222, 3511, 3604, 3784
SfcI	CTRYAG	3 cut(s)	1379, 2462, 2649
SinI	GGWCC	4 cut(s)	358, 888, 2371, 3048
SmaI	CCCGGG	1 cut(s)	2058
SmiMI	CAYNNNNRTG	2 cut(s)	2173, 2775
SmlI	CTYRAG	3 cut(s)	310, 1480, 4105
SmoI	CTYRAG	3 cut(s)	310, 1480, 4105
SspI	AATATT	3 cut(s)	637, 1327, 4215
SspMI	CTAG	7 cut(s)	78, 1503, 1838, 1958, 2031, 2285, 2528
SstI	GAGCTC	2 cut(s)	1288, 3280
StyI	CCWWGG	4 cut(s)	891, 3222, 3814, 3973
TaiI	ACGT	4 cut(s)	1423, 3481, 4079, 4237
TatI	WGTACW	1 cut(s)	3591
TauI	GCSGC	1 cut(s)	517
TscAI	CASTG	7 cut(s)	294, 1234, 1798, 2194, 2353, 3828, 3990
TseFI	GTSAC	5 cut(s)	1148, 2084, 2342, 3080, 3823
TseI	GCWGC	9 cut(s)	517, 961, 1381, 1673, 2569, 3235, 3389, 3524, 3527
Tsp45I	GTSAC	5 cut(s)	1148, 2084, 2342, 3080, 3823
TspGWI	ACGGA	1 cut(s)	739
TspMI	CCCGGG	1 cut(s)	2056
TspRI	CASTG	7 cut(s)	294, 1234, 1798, 2194, 2353, 3828, 3990
Tth111I	GACNNNGTC	1 cut(s)	1357
Vha464I	CTTAAG	1 cut(s)	1480
VpaK11BI	GGWCC	4 cut(s)	358, 888, 2371, 3048
XagI	CCTNNNNNAGG	3 cut(s)	1216, 1392, 3813
XapI	RAATTY	7 cut(s)	137, 499, 836, 1604, 2559, 3204, 4045
XbaI	TCTAGA	1 cut(s)	1837
XceI	RCATGY	1 cut(s)	3497
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1798
XmaI	CCCGGG	1 cut(s)	2056
XmiI	GTMKAC	3 cut(s)	871, 2184, 3711
XmnI	GAANNNNTTC	2 cut(s)	20, 1326
XspI	CTAG	7 cut(s)	78, 1503, 1838, 1958, 2031, 2285, 2528

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.