Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0012193

Physical Location & Seq

Genomic Coordinates Reverse (-)

10766696 .. 10772189

Gene Structure 5494 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1118300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4179 bp

ATGGCAATTGAGAAGAATAACTTCAAGGTTTCGAGGCTTGATTCGGAGTTTTCTCCCAGTAGTAGGAAGAGTATGTCTAGTGATGAGGATGAGCTACAACAGCGTAGCTCTGCTGCGGAATCGGATGACGATGATGAATTTGATGATGCGGACTCGGGAGCGGGGTCTGATGACTTTGATTTGTTGGAATTAGGGGAAACTGGTGTGGAGTTTTGCCAAGTTGGGAACCAGACTCGTAGCATTCCTTTCGAGCTGTATGATATTCCAAGCCTTGAAGATATATTGTCAGTGGATGTATGGAATGAGTGCTTGAGTGAGGAGGAGCAGTTTGGCCTCACTAAGTATCTGCCTGATATGGACCAGGAGACCTTTATGATTACCTTGAAAGAGCTTTTTACGGGTTGCAACTTTCATTTTGGGAGCCCTGTTAAGCAGTTGTTCCATATGTTGAAGGGAGGTTTGTGTGAACCAAGAGTTGCTCTTTACCGGGAGGGTTTGAATTTTTTTCAGAAGCGGCAGCACTACAATCTTTTGAGGAAGCATCAGAACAGTATGGTTAGTAATCTCTGTCAAATAAGGGATGCTTGGCTTAATTGCAAGGGATATAGTATTGAGGAGAGGCTCCGGGTGCTGAATATTATGAGAATCCAGAAGAGTTTGATGGGTGAGAAGATGGAAGATATGGAGGCTGACTCATCAGAAAGAGAGTCGGGTGAAGGTTTACGGAGCGACAAAATCAAGGACAGGAAAACTGCACAGAAAATGGCTCGTTATTCTCCATACGGGGTGGATACAAGTGTGGAACTTGCATCAAAAGGGCGGTCGTCAGCTATGGACTTGGCAAAATATGGGAAGCAGAATCCGAAAGGTATACTGAAGTTGGCTGGGTCCAAGACTCCTTCAGCAAAAGAGCTAGCAAACCATTCAGGACCATACAGTTCAGCCGTCGCTCTTCCTCGGCAGCATAAAGCAGTAGGGGATGATGCCGGGGCAGCACTCAGAATAAGGGATCAATTTATAAGTGGTGATGATGTTGAAGACGCAACATATGGATTTGACATTCAGCGAGATAGAAATGTGTCTCGTGGTAGCAGTATGGATAGGTCTGGTGTTTTCAAAGTGGGAAAGAATCATGACCTTTTAAGAGGCGATGAATTAAACACTGACAGTTTGATGGGTCTGCCTCTCTCCTCAAAGGCTGACGTTTATGCCTATGGTAGGAACCGCAGTGGAAACCTTTTGTCAGAGGCAAATGTTTTAACAGCAAAGCCTCCTAACTTGAGAGCTCCCTATGAATTTGGTAAAAAGGCCAAGTATCCAGAAAATATTCATCAATTCACAGCTGGGGATCAGATGAAGTCCTTGAAAGCCAGACTTCCACAGCCACCGCTCAGAGGAGATCAAGCTGACTTATCAGAACGTGCAGAACCATTCTGGCATAAAAGGACAGAAGGGGATACTTTTTCTATGGATTCTCCCTTAAGAGCCGATGATTGGAATGCTAGGAGCAAGAAATGGAAGTTAGGGAGGGAGCCTCCTGATCTAAATCACAAATCATATAGAGCTTCCCCACCACAGAGGAATGCTAGGTTTATATCTTCTGAATTTAGAGCAAAACCATTGCAAGAGAAGATGAGAGATAAGAGAATGCAAAATGGAGGATCAGAAATGGCAGCATTGAAAGGTAATAGAATGTTTGTAAAAAATGAAGATACGGAATCAGACTCATCGGAGCAATTTGACGACGATGAGGATAGCAACCCTTTGTTGAGGAGGAAGCTGGCTTATCCCAGTGGTGCTATGGAAACTTCTCCATCTTTGTTGAATCCCACTCTAGAAGCAAAAAGGACCAAATATGCGAAGAAAGAGGTGAAGGAGAGTTTCCAGGCTCTTGATGGGATCAATTACTCCAGTAAGATGGGTGGTTTTGCTGAACATGGACATATGCGTAATCGAGAGAACTATTCTTCCAAAGCAAAACAAAAGGGTAAGATGCGAGACAATAGTCCCTTACATAACTCTTCGACTAGAGCTTTCAAAGAGTGTTATATCCCGGGCTTGAGTAAATTTAATGATGAAGGTGATGATTATGATGAACAGAAACAAATCTACAAATTGGGCAAGAATGCCCAATTTCAAGGGGAAGCTGGTGAAAGTTTGCACACACCCTCATGGAAGGTCTACACTGGAAAGCAGAAAAGAGAAGTTGCCCATGATCATTCTGTGCCAGAGTCTCACTATTTTGTTGACGAGGAGGATGATTCACTTGGAATGCAGTTCCTAGGGAATGGTGGTGGACGGGGTAATATCAGGAAGAAAGATCAGAACATTGAAGAATATGTGAGTGATCGCCATGAAAGAATTGAGGTCCCATTATTGGGGTGCAACATGATGGCAAAGAAGCGGCAAGGGAAGGAGGATGTGTCGGATACTGGTAGAGGAGATGAAGGTGGCGACCTCCAGTCTAACCATAAACAGCTCATTGTTGATTCCAGTTCCTTTAAAAAAAAGGCAAAGAGGAAGCTAGAGAATGAGACTGTTAGTTCAGATGTAGAAATTTCTGAGCAACCAATTACAGAAATGGGAGCAACGGATATGGAGCCGGAAACCAGGCCCCAGAAAAAGCCATTTGCTCCAATTACACCTACAGTTCATACTGGTTTCTCATTCTCGATTATACATCTTCTTTCAGCTGTTCGCTTGGCAATGATCACTGCAGTCCCAGAAGGTACTGTTGGGGAATCTGTCGATGAACCAAACAAAACTCACGAGGGTGCTGTCAATGGGGTTCTTTCTTGTGAAAAGCCAGATGTCAATAACTTAGAGCTTGCTGGAGAAATGAATATGCCTTTTCTAACGGTTCAGGAGATTGTGAACCGTGTTAGCTTGAATCCAGGAGATCCTTGTATTCTTGAGACACAAGAGCCACTTCAGGATCTTGTGAGAGGAGTTCTAAGGATCTTTTCATCAAAAACAGCACCTTTAGGAGCAAAAGGTTGGAAGACACTGGTTGCCTTTGAAAAAGCTACAAAAAGCTGGTCATGGACTGGTCCAGTTTCTCAGAGTTCATCAGATCACGACGCCAATGAGGAGGTGATATATCCTGAAGCATGGGGTCTTCCGCACAAAATGCTTGTCAAGCTGGTGGATTCGTTTGCAAATTGGCTCAAATGTGGGCAAGATACCATTCAACAAATTGGAATTCTTCCGGCACCGCCCTTGGAGTTGATGCAGCTCAACCTGGATGAGAAAGAAAGGTTCAGGGATTTAAGAGCTCAAAAGAGTCTTAACACCATTAGCCCAAGTTCCGAAGTAGTGAGGGCTTATTTCCGTAAGGAGGAGGTTCTCAGGTATTCAATTCCAGACAGGGCCTTTTCTTACACAGCAGCTGATGGTAAAAAATCCATTGTTGCCCCATTGAGAAGGTGCGGTGGTAAGCCAACATCAAAGGCCCGAGATCACTTTATGCTGAAACGTGATCGACCACCACATGTTACAATTCTTTGTCTTGTGAGAGATGCAGCTGCAAGGTTGCCTGGAAGTATTGGCACCAGAGCAGATGTTTGCACTTTAATAAGAGATTCTCAGTACATTGTTGAAGATGTGTCTGATGCACAAGTTAATCAAGTTGTTAGTGGTGCCCTGGATCGGTTGCATTATGAGCGTGATCCATGTGTGCAATTTGATGGGGAAAGGAAATTATGGGTCTATTTACATAGAGAAAGAGAAGAAGAAGATTTTGAGGATGACGGTACTTCATCTACAAAGAAATGGAAGAGGCAGAAAAAGGATGCTGGTGATCTACCTGACAAAGGAGCAGTGACTGTGGCTTATCATGGGACTGAGGAACAAACCGGATATGATATGTGCTCTGATCTCAATGTCGAGCCATCATGTTTGGATGATATGCAACAAGATGTGGAGGATAATACTGATACCAATAACGGGTCTGAACAAGATGAAATGCGCCAAGGCAATCCAATGCTTTGGGAGGGTCATGGCTTAAATCCAATGTGCGAAAACAAATTGCTGTGTCAGGAAAATTCCACAAATGAAGATTTTGATGATGAAACGTTTGGGAGAGAAAGGACAGTTGGGCTCTTGAGTGCAAGCTTATTGCATAGCACTCCATCCACCTTCCCATCAGATCGCATTGTTGTTCCCGGTCAAGGAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0002020 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0019899 GO:0031011 GO:0031974 GO:0031981 GO:0032991 GO:0033202 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0070603 GO:0097346 GO:1902494 GO:1904949

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1393

Amino Acids

156.31

Weight (kDa)

5.49

Isoelectric Point (pI)

45.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_2nd_NFRKB	PF25793	1107 - 1245	7.2e-65	NFRKB second winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45830
fragaria_vesca	FvH4_3g34430 FvH4_3g34430
malus_domestica	MD03G1106200.v1.1 MD11G1118300.v1.1
prunus_persica	Prupe.6G088700_v2.0.a1
pyrus_communis	pycom03g08520 pycom11g09930
rosa_chinensis	RchiOBHm_Chr5g0062031
rosa_laevigata	RLG00000035527
rosa_multiflora	Rmu_sc0002296.1_g000008 Rmu_sc0017669.1_g000005
rosa_roxburghii	Rroxscaffold_1G00018660
rosa_rugosa	Rorug05G0345700
rosa_samantha	Rh5AG407000 Rh5BG420300 Rh5CG444400 Rh5DG435700
rosa_wichuraiana	Rw5G038350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1019
AccB1I	GGYRCC	3 cut(s)	3211, 3548, 3638
AccBSI	CCGCTC	2 cut(s)	161, 1390
AccI	GTMKAC	2 cut(s)	871, 2181
AclI	AACGTT	1 cut(s)	4073
AclWI	GGATC	9 cut(s)	1017, 1356, 1669, 1907, 2894, 2943, 2966, 3654, 3662
AcsI	RAATTY	8 cut(s)	137, 499, 1295, 1604, 2066, 2556, 3201, 4042
AcuI	CTGAAG	4 cut(s)	885, 896, 2915, 3126
AcyI	GRCGYC	1 cut(s)	3081
AfaI	GTAC	3 cut(s)	2731, 3590, 3754
AflII	CTTAAG	1 cut(s)	1480
AflIII	ACRYGT	1 cut(s)	3490
AhdI	GACNNNNNGTC	1 cut(s)	2461
AjnI	CCWGG	7 cut(s)	360, 1884, 2609, 2893, 3240, 3535, 3642
AjuI	GAANNNNNNNTTGG	4 cut(s)	2718, 2750, 4077, 4109
AleI	CACNNNNGTG	1 cut(s)	2772
AloI	GAACNNNNNNTCC	2 cut(s)	1952, 1984
Alw21I	GWGCWC	3 cut(s)	1288, 3277, 3872
Alw26I	GTCTC	6 cut(s)	359, 1086, 1992, 2238, 2528, 2909
AlwI	GGATC	9 cut(s)	1017, 1356, 1669, 1907, 2894, 2943, 2966, 3654, 3662
AlwNI	CAGNNNCTG	2 cut(s)	3389, 3824
Ama87I	CYCGRG	3 cut(s)	154, 2053, 3453
AoxI	GGCC	5 cut(s)	331, 1308, 2612, 3369, 3450
ApeKI	GCWGC	9 cut(s)	113, 517, 961, 992, 1673, 3232, 3386, 3521, 3524
ApoI	RAATTY	8 cut(s)	137, 499, 1295, 1604, 2066, 2556, 3201, 4042
ArsI	GACNNNNNNTTYG	6 cut(s)	806, 838, 3023, 3055, 3120, 3152
Asp700I	GAANNNNTTC	2 cut(s)	20, 1326
AspA2I	CCTAGG	1 cut(s)	2281
AspLEI	GCGC	1 cut(s)	3969
AspS9I	GGNCC	9 cut(s)	358, 888, 929, 1848, 2368, 2613, 3050, 3369, 3451
AsuC2I	CCSGG	6 cut(s)	488, 626, 988, 2054, 2055, 4164
AsuHPI	GGTGA	8 cut(s)	677, 725, 1037, 1882, 2093, 2162, 3106, 3809
AsuNHI	GCTAGC	1 cut(s)	913
AvaI	CYCGRG	3 cut(s)	154, 2053, 3453
AvaII	GGWCC	6 cut(s)	358, 888, 929, 1848, 2368, 3050
AvrII	CCTAGG	1 cut(s)	2281
BaeGI	GKGCMC	1 cut(s)	3643
BaeI	ACNNNNGTAYC	2 cut(s)	783, 816
BanI	GGYRCC	3 cut(s)	3211, 3548, 3638
BanII	GRGCYC	4 cut(s)	425, 1288, 3277, 4101
BauI	CACGAG	2 cut(s)	1083, 2768
BbsI	GAAGAC	3 cut(s)	1044, 3008, 3110
Bbv12I	GWGCWC	3 cut(s)	1288, 3277, 3872
BbvI	GCAGC	9 cut(s)	100, 529, 973, 1004, 1685, 3244, 3398, 3511, 3533
BceAI	ACGGC	1 cut(s)	929
BcgI	CGANNNNNNTGC	2 cut(s)	229, 263
BciT130I	CCWGG	7 cut(s)	362, 1886, 2611, 2895, 3242, 3537, 3644
BciVI	GTATCC	4 cut(s)	784, 1326, 1450, 2422
BclI	TGATCA	2 cut(s)	2215, 2709
BcnI	CCSGG	6 cut(s)	488, 626, 988, 2054, 2055, 4164
BcoDI	GTCTC	6 cut(s)	359, 1086, 1992, 2238, 2528, 2909
BfaI	CTAG	8 cut(s)	78, 914, 1503, 1587, 1835, 2028, 2282, 2525
BfmI	CTRYAG	2 cut(s)	2646, 2715
BfrI	CTTAAG	1 cut(s)	1480
BfuI	GTATCC	4 cut(s)	784, 1326, 1450, 2422
BlnI	CCTAGG	1 cut(s)	2281
Bme18I	GGWCC	6 cut(s)	358, 888, 929, 1848, 2368, 3050
BmeRI	GACNNNNNGTC	1 cut(s)	2461
BmeT110I	CYCGRG	3 cut(s)	154, 2053, 3453
BmgT120I	GGNCC	9 cut(s)	358, 888, 929, 1848, 2368, 2613, 3050, 3369, 3451
BmrI	ACTGGG	2 cut(s)	51, 1785
BmtI	GCTAGC	1 cut(s)	917
BmuI	ACTGGG	2 cut(s)	51, 1785
BoxI	GACNNNNGTC	1 cut(s)	2004
BpiI	GAAGAC	3 cut(s)	1044, 3008, 3110
BplI	GAGNNNNNCTC	4 cut(s)	677, 709, 1519, 1551
BpmI	CTGGAG	3 cut(s)	1894, 2444, 2853
BpuEI	CTTGAG	5 cut(s)	331, 1300, 2080, 2933, 4123
BpuMI	CCSGG	6 cut(s)	488, 626, 988, 2054, 2055, 4164
BsaBI	GATNNNNATC	1 cut(s)	1545
BsaHI	GRCGYC	1 cut(s)	3081
BsaI	GGTCTC	1 cut(s)	359
BsaJI	CCNNGG	7 cut(s)	956, 987, 2053, 2281, 3219, 3642, 3970
BsaWI	WCCGGW	1 cut(s)	3854
BsaXI	ACNNNNNCTCC	4 cut(s)	311, 341, 2408, 2438
Bse3DI	GCAATG	2 cut(s)	1619, 2712
Bse8I	GATNNNNATC	1 cut(s)	1545
BseBI	CCWGG	7 cut(s)	362, 1886, 2611, 2895, 3242, 3537, 3644
BseDI	CCNNGG	7 cut(s)	956, 987, 2053, 2281, 3219, 3642, 3970
BseJI	GATNNNNATC	1 cut(s)	1545
BseMI	GCAATG	2 cut(s)	1619, 2712
BseMII	CTCAG	7 cut(s)	1012, 1405, 2553, 3074, 3361, 3599, 3834
BseSI	GKGCMC	1 cut(s)	3643
BseXI	GCAGC	9 cut(s)	100, 529, 973, 1004, 1685, 3244, 3398, 3511, 3533
BseYI	CCCAGC	2 cut(s)	884, 1343
BsgI	GTGCAG	2 cut(s)	738, 1443
Bsh1285I	CGRYCG	1 cut(s)	824
BshFI	GGCC	5 cut(s)	333, 1310, 2614, 3371, 3452
BshNI	GGYRCC	3 cut(s)	3211, 3548, 3638
BsiEI	CGRYCG	1 cut(s)	824
BsiHKAI	GWGCWC	3 cut(s)	1288, 3277, 3872
BsiHKCI	CYCGRG	3 cut(s)	154, 2053, 3453
BsiSI	CCGG	8 cut(s)	487, 625, 987, 2054, 2603, 3209, 3855, 4164
BslFI	GGGAC	4 cut(s)	1992, 2354, 2705, 3853
BsmAI	GTCTC	6 cut(s)	359, 1086, 1992, 2238, 2528, 2909
BsmFI	GGGAC	4 cut(s)	1992, 2354, 2705, 3853
BsmI	GAATGC	6 cut(s)	240, 1504, 1588, 1653, 2131, 2277
BsnI	GGCC	5 cut(s)	333, 1310, 2614, 3371, 3452
Bso31I	GGTCTC	1 cut(s)	359
BsoBI	CYCGRG	3 cut(s)	154, 2053, 3453
Bsp1286I	GDGCHC	6 cut(s)	425, 1288, 3277, 3643, 3872, 4101
BspANI	GGCC	5 cut(s)	333, 1310, 2614, 3371, 3452
BspCNI	CTCAG	7 cut(s)	1011, 1404, 2554, 3073, 3360, 3598, 3835
BspHI	TCATGA	1 cut(s)	1132
BspMAI	CTGCAG	1 cut(s)	2719
BspOI	GCTAGC	1 cut(s)	917
BspPI	GGATC	9 cut(s)	1017, 1356, 1669, 1907, 2894, 2943, 2966, 3654, 3662
BspQI	GCTCTTC	1 cut(s)	957
BspT107I	GGYRCC	3 cut(s)	3211, 3548, 3638
BspTI	CTTAAG	1 cut(s)	1480
BspTNI	GGTCTC	1 cut(s)	359
BsrBI	CCGCTC	2 cut(s)	161, 1390
BsrDI	GCAATG	2 cut(s)	1619, 2712
BssECI	CCNNGG	7 cut(s)	956, 987, 2053, 2281, 3219, 3642, 3970
BssNAI	GTATAC	1 cut(s)	872
BssNI	GRCGYC	1 cut(s)	3081
BssSI	CACGAG	2 cut(s)	1083, 2768
BssT1I	CCWWGG	3 cut(s)	2281, 3219, 3970
Bst1107I	GTATAC	1 cut(s)	872
Bst2BI	CACGAG	2 cut(s)	1083, 2768
Bst2UI	CCWGG	7 cut(s)	362, 1886, 2611, 2895, 3242, 3537, 3644
Bst6I	CTCTTC	5 cut(s)	62, 647, 957, 2026, 3770
BstACI	GRCGYC	1 cut(s)	3081
BstAFI	CTTAAG	1 cut(s)	1480
BstAPI	GCANNNNNTGC	1 cut(s)	3130
BstC8I	GCNNGC	4 cut(s)	915, 1782, 2829, 4111
BstENI	CCTNNNNNAGG	2 cut(s)	1216, 3810
BstHHI	GCGC	1 cut(s)	3969
BstMAI	GTCTC	6 cut(s)	359, 1086, 1992, 2238, 2528, 2909
BstMCI	CGRYCG	1 cut(s)	824
BstMWI	GCNNNNNNNGC	6 cut(s)	100, 628, 992, 3130, 3139, 3661
BstNI	CCWGG	7 cut(s)	362, 1886, 2611, 2895, 3242, 3537, 3644
BstNSI	RCATGY	1 cut(s)	3494
BstPAI	GACNNNNGTC	1 cut(s)	2004
BstSFI	CTRYAG	2 cut(s)	2646, 2715
BstSLI	GKGCMC	1 cut(s)	3643
BstV1I	GCAGC	9 cut(s)	100, 529, 973, 1004, 1685, 3244, 3398, 3511, 3533
BstV2I	GAAGAC	3 cut(s)	1044, 3008, 3110
BstX2I	RGATCY	3 cut(s)	2899, 2935, 2958
BstXI	CCANNNNNNTGG	2 cut(s)	1918, 3987
BstYI	RGATCY	3 cut(s)	2899, 2935, 2958
BstZ17I	GTATAC	1 cut(s)	872
BsuI	GTATCC	4 cut(s)	784, 1326, 1450, 2422
BsuRI	GGCC	5 cut(s)	333, 1310, 2614, 3371, 3452
BtgZI	GCGATG	1 cut(s)	1164
BtsI	GCAGTG	3 cut(s)	1234, 2712, 3825
BtsIMutI	CAGTG	8 cut(s)	294, 1161, 1234, 1798, 2184, 2712, 3005, 3825
Cac8I	GCNNGC	4 cut(s)	915, 1782, 2829, 4111
CaiI	CAGNNNCTG	2 cut(s)	3389, 3824
CciI	TCATGA	1 cut(s)	1132
CfoI	GCGC	1 cut(s)	3969
Cfr13I	GGNCC	9 cut(s)	358, 888, 929, 1848, 2368, 2613, 3050, 3369, 3451
Cfr9I	CCCGGG	1 cut(s)	2053
CseI	GACGC	2 cut(s)	1049, 3089
Csp6I	GTAC	3 cut(s)	2730, 3589, 3753
CviQI	GTAC	3 cut(s)	2730, 3589, 3753
DraI	TTTAAA	1 cut(s)	2503
DriI	GACNNNNNGTC	1 cut(s)	2461
Eam1104I	CTCTTC	5 cut(s)	62, 647, 957, 2026, 3770
Eam1105I	GACNNNNNGTC	1 cut(s)	2461
EarI	CTCTTC	5 cut(s)	62, 647, 957, 2026, 3770
Ecl136II	GAGCTC	2 cut(s)	1286, 3275
Eco130I	CCWWGG	3 cut(s)	2281, 3219, 3970
Eco24I	GRGCYC	4 cut(s)	425, 1288, 3277, 4101
Eco31I	GGTCTC	1 cut(s)	359
Eco47I	GGWCC	6 cut(s)	358, 888, 929, 1848, 2368, 3050
Eco53kI	GAGCTC	2 cut(s)	1286, 3275
Eco57I	CTGAAG	4 cut(s)	885, 896, 2915, 3126
Eco88I	CYCGRG	3 cut(s)	154, 2053, 3453
EcoICRI	GAGCTC	2 cut(s)	1286, 3275
EcoNI	CCTNNNNNAGG	2 cut(s)	1216, 3810
EcoO109I	RGGNCCY	3 cut(s)	2368, 2613, 3369
EcoRI	GAATTC	1 cut(s)	3201
EcoRII	CCWGG	7 cut(s)	360, 1884, 2609, 2893, 3240, 3535, 3642
EcoT14I	CCWWGG	3 cut(s)	2281, 3219, 3970
EcoT38I	GRGCYC	4 cut(s)	425, 1288, 3277, 4101
ErhI	CCWWGG	3 cut(s)	2281, 3219, 3970
FalI	AAGNNNNNCTT	7 cut(s)	37, 568, 600, 1359, 1391, 2913, 2945
FaqI	GGGAC	4 cut(s)	1992, 2354, 2705, 3853
FauI	CCCGC	1 cut(s)	154
FauNDI	CATATG	3 cut(s)	444, 1048, 1944
FbaI	TGATCA	2 cut(s)	2215, 2709
FblI	GTMKAC	2 cut(s)	871, 2181
FriOI	GRGCYC	4 cut(s)	425, 1288, 3277, 4101
FspBI	CTAG	8 cut(s)	78, 914, 1503, 1587, 1835, 2028, 2282, 2525
GlaI	GCGC	1 cut(s)	3968
GsaI	CCCAGC	2 cut(s)	888, 1347
GsuI	CTGGAG	3 cut(s)	1894, 2444, 2853
HaeIII	GGCC	5 cut(s)	333, 1310, 2614, 3371, 3452
HapII	CCGG	8 cut(s)	487, 625, 987, 2054, 2603, 3209, 3855, 4164
HgaI	GACGC	2 cut(s)	1049, 3089
HhaI	GCGC	1 cut(s)	3969
Hin1I	GRCGYC	1 cut(s)	3081
Hin6I	GCGC	1 cut(s)	3967
HinP1I	GCGC	1 cut(s)	3967
HincII	GTYRAC	1 cut(s)	2248
HindII	GTYRAC	1 cut(s)	2248
HindIII	AAGCTT	1 cut(s)	4111
HpaII	CCGG	8 cut(s)	487, 625, 987, 2054, 2603, 3209, 3855, 4164
HphI	GGTGA	8 cut(s)	677, 725, 1037, 1882, 2093, 2162, 3106, 3809
Hpy166II	GTNNAC	7 cut(s)	467, 722, 872, 2182, 2248, 2297, 2875
Hpy8I	GTNNAC	7 cut(s)	467, 722, 872, 2182, 2248, 2297, 2875
Hpy99I	CGWCG	3 cut(s)	950, 1748, 3083
HpyCH4IV	ACGT	4 cut(s)	1203, 1420, 3475, 4073
HpyF10VI	GCNNNNNNNGC	6 cut(s)	100, 628, 992, 3130, 3139, 3661
HpySE526I	ACGT	4 cut(s)	1203, 1420, 3475, 4073
Hsp92I	GRCGYC	1 cut(s)	3081
HspAI	GCGC	1 cut(s)	3967
Ksp22I	TGATCA	2 cut(s)	2215, 2709
LguI	GCTCTTC	1 cut(s)	957
Lsp1109I	GCAGC	9 cut(s)	100, 529, 973, 1004, 1685, 3244, 3398, 3511, 3533
MaeI	CTAG	8 cut(s)	78, 914, 1503, 1587, 1835, 2028, 2282, 2525
MaeII	ACGT	4 cut(s)	1203, 1420, 3475, 4073
MaeIII	GTNAC	2 cut(s)	3493, 3820
MbiI	CCGCTC	2 cut(s)	161, 1390
MfeI	CAATTG	1 cut(s)	6
MflI	RGATCY	3 cut(s)	2899, 2935, 2958
MhlI	GDGCHC	6 cut(s)	425, 1288, 3277, 3643, 3872, 4101
MlyI	GAGTC	8 cut(s)	146, 226, 686, 716, 889, 1718, 2240, 3292
MmeI	TCCRAC	3 cut(s)	165, 2406, 2978
MroXI	GAANNNNTTC	2 cut(s)	20, 1326
MslI	CAYNNNNRTG	2 cut(s)	2170, 2772
MspA1I	CMGCKG	4 cut(s)	1343, 2693, 3389, 3524
MspCI	CTTAAG	1 cut(s)	1480
MspI	CCGG	8 cut(s)	487, 625, 987, 2054, 2603, 3209, 3855, 4164
MunI	CAATTG	1 cut(s)	6
Mva1269I	GAATGC	6 cut(s)	240, 1504, 1588, 1653, 2131, 2277
MvaI	CCWGG	7 cut(s)	362, 1886, 2611, 2895, 3242, 3537, 3644
MwoI	GCNNNNNNNGC	6 cut(s)	100, 628, 992, 3130, 3139, 3661
NciI	CCSGG	6 cut(s)	488, 626, 988, 2054, 2055, 4164
NdeI	CATATG	3 cut(s)	444, 1048, 1944
NheI	GCTAGC	1 cut(s)	913
NmeAIII	GCCGAG	1 cut(s)	937
NmuCI	GTSAC	1 cut(s)	3820
NspI	RCATGY	1 cut(s)	3494
OliI	CACNNNNGTG	1 cut(s)	2772
PagI	TCATGA	1 cut(s)	1132
PciI	ACATGT	1 cut(s)	3490
PciSI	GCTCTTC	1 cut(s)	957
PctI	GAATGC	6 cut(s)	240, 1504, 1588, 1653, 2131, 2277
PdmI	GAANNNNTTC	2 cut(s)	20, 1326
PfoI	TCCNGGA	1 cut(s)	2893
PleI	GAGTC	8 cut(s)	146, 226, 686, 715, 889, 1718, 2239, 3291
PpsI	GAGTC	8 cut(s)	146, 226, 686, 715, 889, 1718, 2239, 3291
PpuMI	RGGWCCY	1 cut(s)	2368
PscI	ACATGT	1 cut(s)	3490
PshAI	GACNNNNGTC	1 cut(s)	2004
PsiI	TTATAA	1 cut(s)	1019
Psp124BI	GAGCTC	2 cut(s)	1288, 3277
Psp1406I	AACGTT	1 cut(s)	4073
Psp5II	RGGWCCY	1 cut(s)	2368
Psp6I	CCWGG	7 cut(s)	360, 1884, 2609, 2893, 3240, 3535, 3642
PspFI	CCCAGC	2 cut(s)	884, 1343
PspGI	CCWGG	7 cut(s)	360, 1884, 2609, 2893, 3240, 3535, 3642
PspPI	GGNCC	9 cut(s)	358, 888, 929, 1848, 2368, 2613, 3050, 3369, 3451
PspPPI	RGGWCCY	1 cut(s)	2368
PstI	CTGCAG	1 cut(s)	2719
PstNI	CAGNNNCTG	2 cut(s)	3389, 3824
PsuI	RGATCY	3 cut(s)	2899, 2935, 2958
PvuII	CAGCTG	4 cut(s)	1343, 2693, 3389, 3524
RsaI	GTAC	3 cut(s)	2731, 3590, 3754
RsaNI	GTAC	3 cut(s)	2730, 3589, 3753
RseI	CAYNNNNRTG	2 cut(s)	2170, 2772
SacI	GAGCTC	2 cut(s)	1288, 3277
SapI	GCTCTTC	1 cut(s)	957
Sau96I	GGNCC	9 cut(s)	358, 888, 929, 1848, 2368, 2613, 3050, 3369, 3451
SchI	GAGTC	8 cut(s)	146, 226, 686, 716, 889, 1718, 2240, 3292
SduI	GDGCHC	6 cut(s)	425, 1288, 3277, 3643, 3872, 4101
SfcI	CTRYAG	2 cut(s)	2646, 2715
SinI	GGWCC	6 cut(s)	358, 888, 929, 1848, 2368, 3050
SmaI	CCCGGG	1 cut(s)	2055
SmiMI	CAYNNNNRTG	2 cut(s)	2170, 2772
SmlI	CTYRAG	6 cut(s)	310, 1279, 1480, 2059, 2912, 4102
SmoI	CTYRAG	6 cut(s)	310, 1279, 1480, 2059, 2912, 4102
SspI	AATATT	2 cut(s)	637, 1327
SspMI	CTAG	8 cut(s)	78, 914, 1503, 1587, 1835, 2028, 2282, 2525
SstI	GAGCTC	2 cut(s)	1288, 3277
StyI	CCWWGG	3 cut(s)	2281, 3219, 3970
TaiI	ACGT	4 cut(s)	1206, 1423, 3478, 4076
TaqI	TCGA	8 cut(s)	32, 249, 1954, 2024, 2672, 2748, 3481, 3885
TatI	WGTACW	1 cut(s)	3588
TauI	GCSGC	2 cut(s)	517, 2407
TscAI	CASTG	8 cut(s)	294, 1168, 1234, 1798, 2191, 2719, 3012, 3825
TseFI	GTSAC	1 cut(s)	3820
TseI	GCWGC	9 cut(s)	113, 517, 961, 992, 1673, 3232, 3386, 3521, 3524
Tsp45I	GTSAC	1 cut(s)	3820
TspGWI	ACGGA	4 cut(s)	739, 1730, 2606, 3320
TspMI	CCCGGG	1 cut(s)	2053
TspRI	CASTG	8 cut(s)	294, 1168, 1234, 1798, 2191, 2719, 3012, 3825
Vha464I	CTTAAG	1 cut(s)	1480
VpaK11BI	GGWCC	6 cut(s)	358, 888, 929, 1848, 2368, 3050
XagI	CCTNNNNNAGG	2 cut(s)	1216, 3810
XapI	RAATTY	8 cut(s)	137, 499, 1295, 1604, 2066, 2556, 3201, 4042
XbaI	TCTAGA	1 cut(s)	1834
XceI	RCATGY	1 cut(s)	3494
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1798, 1892
XmaI	CCCGGG	1 cut(s)	2053
XmaJI	CCTAGG	1 cut(s)	2281
XmiI	GTMKAC	2 cut(s)	871, 2181
XmnI	GAANNNNTTC	2 cut(s)	20, 1326
XspI	CTAG	8 cut(s)	78, 914, 1503, 1587, 1835, 2028, 2282, 2525

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.