Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0012193

Physical Location & Seq

Genomic Coordinates Forward (+)

68030416 .. 68035099

Gene Structure 4684 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035527

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4074 bp

ATGGCGATTGAGAAGAATAACTTCAAGGTTTCGAGGTTGGATTCGGAGGTCTCTCCTGCTAGTAGGAAGAGTGTGTCCAGTGACGATGATGAGCTTCAGCAGCGTAGCTCTGCTGCTGAATCTGATGATGATGATGAGTTTGATGATGCTGACTCCGGGGCCGGGTCAGATGACTTTGATTTGTTGGAATTAGGGGAAACGGGTGTCGAGTATTGCCAGGTTGGGAATCAGACCTGCGGCATTCCTTTCGAGCTGTATGATCTTCCGAGTCTGGAGGATATATTGTCAGTGGATGTGTGGAATGAGTGCTTGAGTGAGGAGGAGCAGTTTGGCCTTACTAAGTATCTGCCTGACATGGACCAGGAGACGTTTATGATTACCCTCAAGGAGCTTTTTACGGGGTGTAACTTCCATTTTGGGAGCCCGGTTACCAAGCTGTTTGATATGTTGAAGGGAGGTTTGTGCGAGCCAAGAGTTGCCCTTTACCGGGAGGGTTTGAATTTCTTTCAGCACCGGCGACACTACAATCTTTTGAGGAAGCATCAGAACACTATGGTTCGTAATCTCTGTCAGATAAGGGATGCTTGGCTTAATTGCAAGGGATACAGTATTGAGGAGAGGCTCCGGGTGTTGAATATAATGAGAATCCAGAAGAGTTTGATGTCTGAGAAGATGGAAGATATGGCCAGTGACTCATCAGAAAGAGATTCAGGTGAAGGTCTACGGAGTAACAAGATCAAGGACAGGAAAGTTGCACAGCAAATCAGTCGTCATTCTCCATATGGGGTGGGCTCGAATATGGATTTTGCATCAAAAGGGCGGTCACCGTCTTTGGAAGTGGCCAAATACGGGAAGCAGAATTCCAAAGGCATACTGAAGTTGGGTGGGTCAAAGACTCCTTTAGAAAAAGAGCCAGTGAGTTATCCTGGACCATACAGTTCCGCAGCTGTTCTTCCTCGGTCGAATAAGCTTGGGGCGTATGATTCTGGGGCAGCTCTCAGAATGAGGGATCAGATGATAAGTAGTGATGATGCTGAAGAAACAGCATATGGAATTGATGTTCAACGGGATCGATTTACGTCTCGTGGTAGCATGCCTGACAAGTCTGGTCTTCTGAAAGTGGGAAAGAATCTTATAAGAGGTAATGATGTAATCGCTGACAGTTTAATTGGTCTGCCTCTCTCTACAAAAAATGATGTGCATGCTTATGCTAGGAACCGGGATGCAAACCTTTTGTCGGAGGCAAAGGTATTGACAGCAAAACCTCCTAACATGAGGGCTCCATATGATTTTGGTATAAAGACCAAGTATCCAGGAAATATTCAGCAATATGCAGTGGGTGATCAGATGAAATTCTTGAAAGGCAGACTTCCACAGGCACCTTTCAGAGGAGATCGATATGATTCATCCGATCATGCTGATCTATTCTGGAATAACAGGAGTGAAGGGGAGGCTTTCGCTATGGAATCCCCCTTTCGAGCAGATGATTGGAGTCTTAGGAGCAAGAAATGGAAGACTGGGAGGGAGTCTCCTGATTTAAATTACAAGTCCTATAGAGCCTCCCCGCCACAGATGAATGATAGATTTTTATCTTCCGAATTTAGAGCAAAACCATTGCAAGGGAAGATAAGAGGGAATAAACTACATAATGGAGGATCAGACATGGTGGCGTTGAAAGGTAATAGAATGTTTGTTAAAAATGAAGAAACAGAATCAGACTCATCAGATCAATTTGAGGATGATGAGGATAGCAACCCTTTGCTGAGGAGCAAGTTGGCTTATCCCAGTGGCGTTATGGAACCTTCTCCATCGTCATTGTTGATGCCCAGTCTAGATGCAAAAAGAGCCAAATATGCGCAAAAAGAGGTGAAGAATATGCAGGCCCTTGAGGGGATCAATTACTCCTCCAAAAAGATGGGTGGTTTTGTTGATCAAGGAAATATGCGTAGTTTAGATAACTATTCTTCCAAAGGAAAACAGAAGGGTAAGATGGGAGACAGTAGTCCCTTGCATTTGGAAGGGCGCTATTTCCCCAGCTTGGATAAACTTGACGACAATGATGATGATGAACTGAAACCGCTCTACAGATTGGGCAAGAATGCAAAACTTCAAGGGGGACCTGGTGAAAGGTTGCATATACCCTCACTCAAGACTTATACTGCTAGTGGAAAGCAGAAACGAGAAGTTGTTCATGACCATTCTGTTTCACAGTCTCAATACTTTGTTGATGAGGAAGATGATTCCCTTCAAATGCGTTTACTGGGAGATGGTAGTGCACAGGGTCGATCCAGAAACAAAGGCCAGAATGTCGAAGGATATTTGAGGGATCACCGTGAAAGCATTGAGGTCCCAATACTTGGCTGCAGTTTGGTGACAAAGAAGCGGAAAGGGAAAGAGGATGCCGTGGATATGAGTAGAGGAGATGAAGACCTACAGTCCAATCATTTGCAACGCAGTGCTGAGTCCCATTCCTTGAAGAAAAAGGTAAAGAGGAAGATGGAGACAGACACTGGTAGTTCAGATATGGAAATTTCTGAGCTCCCAGTTACAGAAATGGGAGCAACAGATATGGAGCTGGAAACCAAGCCCCAGAAAAAGCCATTTATTTTAATTACACCCACTGTTCATACTGGTTTCTCATTCTCGATTATGCATCTTCTTTCAGCAGTTCGCTTGGCAATGATTACTCCACTTTCGGAAGATACTTTAGATGTTGGGGAACCTATAGATGAGAAGAACAAAAATCAGGAAGGTGGCGCCAATGGCGTTATTACTCATGAAAAGGTGGATGCAAATAACGGTGAGCATGCTGGAGAAGGGAGTACGCCTTTTGTAACAGTTCAGGAGATTGTGAATCGTGTTAGATCAAATCCAGGAGATCCTTGCATTCTTGAAACACAAGAACCACTTCAGGATCTGGTGAGAGGAGTTCTGAAGATTTTTTCATCAAAAACAGCACCTTTAGGGGCGAAGGGTTGGAAGCCATTAGCTGCTTATGAAAAAGCTACAAAAAGTTGGTCATGGACTGGCCCAGTCTCTCATAGTTCATCAGATAATGAGACCATTGAGGAGGTAACATCTCCTGAAGCATGGGGTCTACCTCACAAGATGCTTGTCAAGTTGGTTGATTCATTTGCTAATTGGCTCAAATGTGGGCAAGAGACCCTCCAACAAATAGGAAGTCTTCCTGCACCGCCTTTGGAGTTGATGCAGCCCAACGTAGATGAGAAGGAGAGGTTTAGGGACTTAAGAGCTCAAAAGAGTCTTAATACCATCAATCCAAGTTCTGAAGAAGTGAAAGCTTATTTCCGTAAGGAGGAACTTCTTAGGTATTCAGTACCTGACAGGGCCTTCTCTTACACCGCAGCTGATGGTAAAAAATCTATTGTTGCCCCATTGAGAAGATGCGGTGGTAAGCCAACATCAAAAGCCCGAGATCACTTTATGCTGAAACGTGATCGACCGCCGCATGTTACGATCCTTTGTCTTGTGAGAGACGCAGCTGCCAGATTGCCTGGAAGTATTGGAACCAGAGCAGATGTATGTACTTTGATAAGAGATTCTCAGTATATTGTTGAAGATGTGTCTGATACACAAGTGAATCAAGTTGTTAGTGGAGCCCTGGACCGCTTGCATTATGAGCGTGATCCTTGTGTACAGTTTGATGGGGAAAGAAAATTATGGGTCTATTTACATAGAGAAAGAGAAGAAGAAGATTTTGAGGACGATGGTACTTCATCTACAAAGAAATGGAAGAGGCAGAAAAAGGATGCTGCTGATCAAGCTGACCTAGGAGGAGTGACAGTGGCTTATCATGGATCTGAGGAACAATCTGGATATGATTTGTGCTCCGATCTTAATGCAGACCCATCATTTTTGGATGATGACAAGGGAGTGGAACTTGAATATGATGATGTGAGGCAAGATGCAGACGTTGATGTTGATACCAATCAGGGGTCTGAACAAGAAGAAATGCGTCAAGACAATCCACTGAGAAAGGACGGTTGGGCTCTTGAGTGCAAGCTTACTATGAGGTCTTCTTCAGGCATAACGATCCTCTACATCCATCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0002020 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0019899 GO:0031011 GO:0031974 GO:0031981 GO:0032991 GO:0033202 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0070603 GO:0097346 GO:1902494 GO:1904949

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1358

Amino Acids

151.96

Weight (kDa)

5.54

Isoelectric Point (pI)

46.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_2nd_NFRKB	PF25793	1100 - 1238	7e-65	NFRKB second winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45830
fragaria_vesca	FvH4_3g34430 FvH4_3g34430
malus_domestica	MD03G1106200.v1.1 MD11G1118300.v1.1
prunus_persica	Prupe.6G088700_v2.0.a1
pyrus_communis	pycom03g08520 pycom11g09930
rosa_chinensis	RchiOBHm_Chr5g0062031
rosa_laevigata	RLG00000035527
rosa_multiflora	Rmu_sc0002296.1_g000008 Rmu_sc0017669.1_g000005
rosa_roxburghii	Rroxscaffold_1G00018660
rosa_rugosa	Rorug05G0345700
rosa_samantha	Rh5AG407000 Rh5BG420300 Rh5CG444400 Rh5DG435700
rosa_wichuraiana	Rw5G038350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1136
Acc16I	TGCGCA	1 cut(s)	1857
Acc36I	ACCTGC	1 cut(s)	242
AccB1I	GGYRCC	2 cut(s)	1378, 2756
AccB7I	CCANNNNNTGG	1 cut(s)	2357
AccBSI	CCGCTC	1 cut(s)	2080
AccI	GTMKAC	2 cut(s)	721, 3097
AcoI	YGGCCR	2 cut(s)	684, 840
AcsI	RAATTY	5 cut(s)	499, 859, 1352, 1598, 2529
AcuI	CTGAAG	8 cut(s)	80, 896, 1056, 2894, 2954, 3105, 3309, 4026
AcyI	GRCGYC	1 cut(s)	2757
AfaI	GTAC	5 cut(s)	2824, 3339, 3547, 3657, 3733
AflII	CTTAAG	1 cut(s)	3247
AhdI	GACNNNNNGTC	1 cut(s)	2434
AjnI	CCWGG	8 cut(s)	216, 360, 925, 1312, 2119, 2872, 3514, 3621
AjuI	GAANNNNNNNTTGG	2 cut(s)	3988, 4020
AloI	GAACNNNNNNTCC	2 cut(s)	1044, 1076
Alw21I	GWGCWC	4 cut(s)	2278, 2541, 3256, 3851
Alw44I	GTGCAC	1 cut(s)	2274
AlwNI	CAGNNNCTG	3 cut(s)	2510, 2917, 3341
Ama87I	CYCGRG	1 cut(s)	3432
AoxI	GGCC	8 cut(s)	159, 331, 684, 840, 1881, 2299, 3028, 3348
ApaLI	GTGCAC	1 cut(s)	2274
ApoI	RAATTY	5 cut(s)	499, 859, 1352, 1598, 2529
Asp700I	GAANNNNTTC	3 cut(s)	20, 2187, 2907
AspA2I	CCTAGG	1 cut(s)	3790
AspLEI	GCGC	3 cut(s)	1858, 2025, 2759
AspS9I	GGNCC	9 cut(s)	159, 358, 929, 1882, 2117, 2347, 3029, 3348, 3625
AsuC2I	CCSGG	6 cut(s)	157, 163, 425, 488, 626, 1220
AsuHPI	GGTGA	9 cut(s)	725, 816, 1352, 1879, 2135, 2321, 2383, 2813, 2932
AvaI	CYCGRG	1 cut(s)	3432
AvaII	GGWCC	5 cut(s)	358, 929, 2117, 2347, 3625
AvrII	CCTAGG	1 cut(s)	3790
BaeGI	GKGCMC	1 cut(s)	2278
BaeI	ACNNNNGTAYC	2 cut(s)	3582, 3615
BalI	TGGCCA	2 cut(s)	686, 842
BanI	GGYRCC	2 cut(s)	1378, 2756
BanII	GRGCYC	7 cut(s)	425, 794, 1282, 2541, 3256, 3622, 4012
BauI	CACGAG	1 cut(s)	1083
BbsI	GAAGAC	5 cut(s)	1103, 1520, 2433, 3176, 4029
Bbv12I	GWGCWC	4 cut(s)	2278, 2541, 3256, 3851
BbvCI	CCTCAGC	1 cut(s)	1763
BceAI	ACGGC	1 cut(s)	2387
BcgI	CGANNNNNNTGC	6 cut(s)	229, 263, 749, 783, 3842, 3876
BciT130I	CCWGG	8 cut(s)	218, 362, 927, 1314, 2121, 2874, 3516, 3623
BciVI	GTATCC	2 cut(s)	596, 1320
BclI	TGATCA	3 cut(s)	1342, 1930, 3778
BcnI	CCSGG	6 cut(s)	157, 163, 425, 488, 626, 1220
BfaI	CTAG	5 cut(s)	60, 1212, 1832, 2163, 3791
BfmI	CTRYAG	5 cut(s)	1552, 2083, 2362, 2432, 2724
BfoI	RGCGCY	2 cut(s)	2026, 2760
BfrI	CTTAAG	1 cut(s)	3247
BfuAI	ACCTGC	1 cut(s)	242
BfuI	GTATCC	2 cut(s)	596, 1320
BlnI	CCTAGG	1 cut(s)	3790
Bme18I	GGWCC	5 cut(s)	358, 929, 2117, 2347, 3625
BmeRI	GACNNNNNGTC	1 cut(s)	2434
BmeT110I	CYCGRG	1 cut(s)	3432
BmgT120I	GGNCC	9 cut(s)	159, 358, 929, 1882, 2117, 2347, 3029, 3348, 3625
BmrI	ACTGGG	6 cut(s)	1527, 1779, 1821, 2270, 2537, 3026
BmuI	ACTGGG	6 cut(s)	1527, 1779, 1821, 2270, 2537, 3026
BoxI	GACNNNNGTC	1 cut(s)	2001
BpiI	GAAGAC	5 cut(s)	1103, 1520, 2433, 3176, 4029
BplI	GAGNNNNNCTC	2 cut(s)	1513, 1545
BpmI	CTGGAG	2 cut(s)	293, 2832
Bpu10I	CCTNAGC	1 cut(s)	1763
BpuEI	CTTGAG	5 cut(s)	331, 368, 1907, 2132, 4034
BpuMI	CCSGG	6 cut(s)	157, 163, 425, 488, 626, 1220
Bsa29I	ATCGAT	2 cut(s)	1072, 1396
BsaBI	GATNNNNATC	2 cut(s)	1588, 3948
BsaHI	GRCGYC	1 cut(s)	2757
BsaI	GGTCTC	3 cut(s)	55, 3053, 3155
BsaJI	CCNNGG	5 cut(s)	156, 956, 2403, 3621, 3790
BsaXI	ACNNNNNCTCC	4 cut(s)	311, 341, 3789, 3819
Bse118I	RCCGGY	1 cut(s)	513
Bse3DI	GCAATG	2 cut(s)	1613, 2685
Bse8I	GATNNNNATC	2 cut(s)	1588, 3948
BseBI	CCWGG	8 cut(s)	218, 362, 927, 1314, 2121, 2874, 3516, 3623
BseCI	ATCGAT	2 cut(s)	1072, 1396
BseDI	CCNNGG	5 cut(s)	156, 956, 2403, 3621, 3790
BseJI	GATNNNNATC	2 cut(s)	1588, 3948
BseMI	GCAATG	2 cut(s)	1613, 2685
BseMII	CTCAG	8 cut(s)	657, 1012, 1754, 2451, 2526, 3578, 3813, 3983
BseSI	GKGCMC	1 cut(s)	2278
BseYI	CCCAGC	1 cut(s)	2033
BsgI	GTGCAG	1 cut(s)	3174
Bsh1285I	CGRYCG	2 cut(s)	962, 3464
BshFI	GGCC	8 cut(s)	161, 333, 686, 842, 1883, 2301, 3030, 3350
BshNI	GGYRCC	2 cut(s)	1378, 2756
BshVI	ATCGAT	2 cut(s)	1072, 1396
BsiEI	CGRYCG	2 cut(s)	962, 3464
BsiHKAI	GWGCWC	4 cut(s)	2278, 2541, 3256, 3851
BsiHKCI	CYCGRG	1 cut(s)	3432
BsiSI	CCGG	7 cut(s)	156, 162, 425, 487, 514, 625, 1219
BslFI	GGGAC	5 cut(s)	1989, 2130, 2333, 2449, 3257
BsmBI	CGTCTC	3 cut(s)	359, 1086, 3489
BsmFI	GGGAC	5 cut(s)	1989, 2130, 2333, 2449, 3257
BsmI	GAATGC	3 cut(s)	240, 2104, 2886
BsnI	GGCC	8 cut(s)	161, 333, 686, 842, 1883, 2301, 3030, 3350
Bso31I	GGTCTC	3 cut(s)	55, 3053, 3155
BsoBI	CYCGRG	1 cut(s)	3432
Bsp1286I	GDGCHC	9 cut(s)	425, 794, 1282, 2278, 2541, 3256, 3622, 3851, 4012
Bsp1407I	TGTACA	1 cut(s)	3655
BspANI	GGCC	8 cut(s)	161, 333, 686, 842, 1883, 2301, 3030, 3350
BspCNI	CTCAG	8 cut(s)	658, 1011, 1755, 2452, 2527, 3577, 3814, 3984
BspDI	ATCGAT	2 cut(s)	1072, 1396
BspHI	TCATGA	2 cut(s)	2191, 2776
BspMAI	CTGCAG	1 cut(s)	2366
BspMI	ACCTGC	1 cut(s)	242
BspT107I	GGYRCC	2 cut(s)	1378, 2756
BspTI	CTTAAG	1 cut(s)	3247
BspTNI	GGTCTC	3 cut(s)	55, 3053, 3155
BsrBI	CCGCTC	1 cut(s)	2080
BsrDI	GCAATG	2 cut(s)	1613, 2685
BsrFI	RCCGGY	1 cut(s)	513
BsrGI	TGTACA	1 cut(s)	3655
BssAI	RCCGGY	1 cut(s)	513
BssECI	CCNNGG	5 cut(s)	156, 956, 2403, 3621, 3790
BssNI	GRCGYC	1 cut(s)	2757
BssSI	CACGAG	1 cut(s)	1083
BssT1I	CCWWGG	1 cut(s)	3790
Bst2BI	CACGAG	1 cut(s)	1083
Bst2UI	CCWGG	8 cut(s)	218, 362, 927, 1314, 2121, 2874, 3516, 3623
Bst6I	CTCTTC	3 cut(s)	62, 647, 3749
BstACI	GRCGYC	1 cut(s)	2757
BstAFI	CTTAAG	1 cut(s)	3247
BstAUI	TGTACA	1 cut(s)	3655
BstC8I	GCNNGC	7 cut(s)	467, 1094, 1203, 1881, 2808, 3632, 4022
BstDSI	CCRYGG	1 cut(s)	2403
BstEII	GGTNACC	2 cut(s)	427, 822
BstENI	CCTNNNNNAGG	1 cut(s)	1386
BstH2I	RGCGCY	2 cut(s)	2026, 2760
BstHHI	GCGC	3 cut(s)	1858, 2025, 2759
BstMCI	CGRYCG	2 cut(s)	962, 3464
BstMWI	GCNNNNNNNGC	6 cut(s)	100, 1844, 1853, 2763, 3640, 3782
BstNI	CCWGG	8 cut(s)	218, 362, 927, 1314, 2121, 2874, 3516, 3623
BstNSI	RCATGY	4 cut(s)	1096, 1205, 2810, 3473
BstPAI	GACNNNNGTC	1 cut(s)	2001
BstPI	GGTNACC	2 cut(s)	427, 822
BstSFI	CTRYAG	5 cut(s)	1552, 2083, 2362, 2432, 2724
BstSLI	GKGCMC	1 cut(s)	2278
BstV2I	GAAGAC	5 cut(s)	1103, 1520, 2433, 3176, 4029
BstX2I	RGATCY	3 cut(s)	2878, 2914, 3818
BstXI	CCANNNNNNTGG	1 cut(s)	1915
BstYI	RGATCY	3 cut(s)	2878, 2914, 3818
Bsu15I	ATCGAT	2 cut(s)	1072, 1396
BsuI	GTATCC	2 cut(s)	596, 1320
BsuRI	GGCC	8 cut(s)	161, 333, 686, 842, 1883, 2301, 3030, 3350
BsuTUI	ATCGAT	2 cut(s)	1072, 1396
BtgI	CCRYGG	1 cut(s)	2403
BtsI	GCAGTG	2 cut(s)	1341, 2461
BveI	ACCTGC	1 cut(s)	242
Cac8I	GCNNGC	7 cut(s)	467, 1094, 1203, 1881, 2808, 3632, 4022
CaiI	CAGNNNCTG	3 cut(s)	2510, 2917, 3341
CciI	TCATGA	2 cut(s)	2191, 2776
CfoI	GCGC	3 cut(s)	1858, 2025, 2759
Cfr10I	RCCGGY	1 cut(s)	513
Cfr13I	GGNCC	9 cut(s)	159, 358, 929, 1882, 2117, 2347, 3029, 3348, 3625
ClaI	ATCGAT	2 cut(s)	1072, 1396
CseI	GACGC	2 cut(s)	3506, 3965
CsiI	ACCWGGT	1 cut(s)	2119
Csp6I	GTAC	5 cut(s)	2823, 3338, 3546, 3656, 3732
CviQI	GTAC	5 cut(s)	2823, 3338, 3546, 3656, 3732
DinI	GGCGCC	1 cut(s)	2758
DraI	TTTAAA	1 cut(s)	1539
DriI	GACNNNNNGTC	1 cut(s)	2434
EaeI	YGGCCR	2 cut(s)	684, 840
Eam1104I	CTCTTC	3 cut(s)	62, 647, 3749
Eam1105I	GACNNNNNGTC	1 cut(s)	2434
EarI	CTCTTC	3 cut(s)	62, 647, 3749
Ecl136II	GAGCTC	2 cut(s)	2539, 3254
Eco130I	CCWWGG	1 cut(s)	3790
Eco24I	GRGCYC	7 cut(s)	425, 794, 1282, 2541, 3256, 3622, 4012
Eco31I	GGTCTC	3 cut(s)	55, 3053, 3155
Eco47I	GGWCC	5 cut(s)	358, 929, 2117, 2347, 3625
Eco53kI	GAGCTC	2 cut(s)	2539, 3254
Eco57I	CTGAAG	8 cut(s)	80, 896, 1056, 2894, 2954, 3105, 3309, 4026
Eco88I	CYCGRG	1 cut(s)	3432
Eco91I	GGTNACC	2 cut(s)	427, 822
EcoICRI	GAGCTC	2 cut(s)	2539, 3254
EcoNI	CCTNNNNNAGG	1 cut(s)	1386
EcoO109I	RGGNCCY	4 cut(s)	1882, 2117, 2347, 3348
EcoO65I	GGTNACC	2 cut(s)	427, 822
EcoRI	GAATTC	1 cut(s)	859
EcoRII	CCWGG	8 cut(s)	216, 360, 925, 1312, 2119, 2872, 3514, 3621
EcoT14I	CCWWGG	1 cut(s)	3790
EcoT22I	ATGCAT	1 cut(s)	2655
EcoT38I	GRGCYC	7 cut(s)	425, 794, 1282, 2541, 3256, 3622, 4012
EgeI	GGCGCC	1 cut(s)	2758
EheI	GGCGCC	1 cut(s)	2758
ErhI	CCWWGG	1 cut(s)	3790
Esp3I	CGTCTC	3 cut(s)	359, 1086, 3489
FalI	AAGNNNNNCTT	7 cut(s)	37, 568, 600, 1353, 1385, 2892, 2924
FaqI	GGGAC	5 cut(s)	1989, 2130, 2333, 2449, 3257
FauI	CCCGC	1 cut(s)	1572
FauNDI	CATATG	3 cut(s)	781, 1048, 1285
FbaI	TGATCA	3 cut(s)	1342, 1930, 3778
FblI	GTMKAC	2 cut(s)	721, 3097
FriOI	GRGCYC	7 cut(s)	425, 794, 1282, 2541, 3256, 3622, 4012
FspBI	CTAG	5 cut(s)	60, 1212, 1832, 2163, 3791
FspI	TGCGCA	1 cut(s)	1857
GlaI	GCGC	3 cut(s)	1857, 2024, 2758
GsaI	CCCAGC	1 cut(s)	2037
GsuI	CTGGAG	2 cut(s)	293, 2832
HaeII	RGCGCY	2 cut(s)	2026, 2760
HaeIII	GGCC	8 cut(s)	161, 333, 686, 842, 1883, 2301, 3030, 3350
HapII	CCGG	7 cut(s)	156, 162, 425, 487, 514, 625, 1219
HgaI	GACGC	2 cut(s)	3506, 3965
HhaI	GCGC	3 cut(s)	1858, 2025, 2759
Hin1I	GRCGYC	1 cut(s)	2757
Hin6I	GCGC	3 cut(s)	1856, 2023, 2757
HinP1I	GCGC	3 cut(s)	1856, 2023, 2757
HindIII	AAGCTT	3 cut(s)	968, 3300, 4022
HpaII	CCGG	7 cut(s)	156, 162, 425, 487, 514, 625, 1219
HphI	GGTGA	9 cut(s)	725, 816, 1352, 1879, 2135, 2321, 2383, 2813, 2932
Hpy166II	GTNNAC	5 cut(s)	722, 2258, 2276, 3098, 3656
Hpy8I	GTNNAC	5 cut(s)	722, 2258, 2276, 3098, 3656
HpyCH4IV	ACGT	5 cut(s)	368, 1079, 3219, 3454, 3933
HpyF10VI	GCNNNNNNNGC	6 cut(s)	100, 1844, 1853, 2763, 3640, 3782
HpySE526I	ACGT	5 cut(s)	368, 1079, 3219, 3454, 3933
Hsp92I	GRCGYC	1 cut(s)	2757
HspAI	GCGC	3 cut(s)	1856, 2023, 2757
KasI	GGCGCC	1 cut(s)	2756
Ksp22I	TGATCA	3 cut(s)	1342, 1930, 3778
MabI	ACCWGGT	1 cut(s)	2119
MaeI	CTAG	5 cut(s)	60, 1212, 1832, 2163, 3791
MaeII	ACGT	5 cut(s)	368, 1079, 3219, 3454, 3933
MbiI	CCGCTC	1 cut(s)	2080
MflI	RGATCY	3 cut(s)	2878, 2914, 3818
MhlI	GDGCHC	9 cut(s)	425, 794, 1282, 2278, 2541, 3256, 3622, 3851, 4012
MlsI	TGGCCA	2 cut(s)	686, 842
MluNI	TGGCCA	2 cut(s)	686, 842
Mly113I	GGCGCC	1 cut(s)	2757
MlyI	GAGTC	9 cut(s)	146, 277, 686, 889, 1501, 1535, 1712, 2471, 3271
MmeI	TCCRAC	5 cut(s)	18, 165, 1218, 2957, 3193
Mox20I	TGGCCA	2 cut(s)	686, 842
Mph1103I	ATGCAT	1 cut(s)	2655
MroXI	GAANNNNTTC	3 cut(s)	20, 2187, 2907
MscI	TGGCCA	2 cut(s)	686, 842
MseI	TTAA	8 cut(s)	591, 1166, 1538, 1695, 2609, 3248, 3267, 3858
MslI	CAYNNNNRTG	1 cut(s)	1206
Msp20I	TGGCCA	2 cut(s)	686, 842
MspA1I	CMGCKG	3 cut(s)	947, 3368, 3503
MspCI	CTTAAG	1 cut(s)	3247
MspI	CCGG	7 cut(s)	156, 162, 425, 487, 514, 625, 1219
Mva1269I	GAATGC	3 cut(s)	240, 2104, 2886
MvaI	CCWGG	8 cut(s)	218, 362, 927, 1314, 2121, 2874, 3516, 3623
MwoI	GCNNNNNNNGC	6 cut(s)	100, 1844, 1853, 2763, 3640, 3782
NarI	GGCGCC	1 cut(s)	2757
NciI	CCSGG	6 cut(s)	157, 163, 425, 488, 626, 1220
NdeI	CATATG	3 cut(s)	781, 1048, 1285
NmuCI	GTSAC	5 cut(s)	80, 689, 822, 2371, 3799
NsbI	TGCGCA	1 cut(s)	1857
NsiI	ATGCAT	1 cut(s)	2655
NspI	RCATGY	4 cut(s)	1096, 1205, 2810, 3473
PaeI	GCATGC	3 cut(s)	1096, 1205, 2810
PagI	TCATGA	2 cut(s)	2191, 2776
PctI	GAATGC	3 cut(s)	240, 2104, 2886
PdmI	GAANNNNTTC	3 cut(s)	20, 2187, 2907
PflMI	CCANNNNNTGG	1 cut(s)	2357
PfoI	TCCNGGA	3 cut(s)	925, 1312, 2872
PleI	GAGTC	9 cut(s)	146, 276, 686, 889, 1500, 1534, 1712, 2470, 3270
PluTI	GGCGCC	1 cut(s)	2760
PpsI	GAGTC	9 cut(s)	146, 276, 686, 889, 1500, 1534, 1712, 2470, 3270
PpuMI	RGGWCCY	2 cut(s)	2117, 2347
PshAI	GACNNNNGTC	1 cut(s)	2001
PsiI	TTATAA	1 cut(s)	1136
Psp124BI	GAGCTC	2 cut(s)	2541, 3256
Psp5II	RGGWCCY	2 cut(s)	2117, 2347
Psp6I	CCWGG	8 cut(s)	216, 360, 925, 1312, 2119, 2872, 3514, 3621
PspEI	GGTNACC	2 cut(s)	427, 822
PspFI	CCCAGC	1 cut(s)	2033
PspGI	CCWGG	8 cut(s)	216, 360, 925, 1312, 2119, 2872, 3514, 3621
PspPI	GGNCC	9 cut(s)	159, 358, 929, 1882, 2117, 2347, 3029, 3348, 3625
PspPPI	RGGWCCY	2 cut(s)	2117, 2347
PstI	CTGCAG	1 cut(s)	2366
PstNI	CAGNNNCTG	3 cut(s)	2510, 2917, 3341
PsuI	RGATCY	3 cut(s)	2878, 2914, 3818
PvuII	CAGCTG	3 cut(s)	947, 3368, 3503
RsaI	GTAC	5 cut(s)	2824, 3339, 3547, 3657, 3733
RsaNI	GTAC	5 cut(s)	2823, 3338, 3546, 3656, 3732
RseI	CAYNNNNRTG	1 cut(s)	1206
SacI	GAGCTC	2 cut(s)	2541, 3256
SaqAI	TTAA	8 cut(s)	591, 1166, 1538, 1695, 2609, 3248, 3267, 3858
Sau96I	GGNCC	9 cut(s)	159, 358, 929, 1882, 2117, 2347, 3029, 3348, 3625
SchI	GAGTC	9 cut(s)	146, 277, 686, 889, 1501, 1535, 1712, 2471, 3271
SduI	GDGCHC	9 cut(s)	425, 794, 1282, 2278, 2541, 3256, 3622, 3851, 4012
SexAI	ACCWGGT	1 cut(s)	2119
SfcI	CTRYAG	5 cut(s)	1552, 2083, 2362, 2432, 2724
SfoI	GGCGCC	1 cut(s)	2758
SgrAI	CRCCGGYG	1 cut(s)	513
SinI	GGWCC	5 cut(s)	358, 929, 2117, 2347, 3625
SmiI	ATTTAAAT	1 cut(s)	1539
SmiMI	CAYNNNNRTG	1 cut(s)	1206
SmlI	CTYRAG	6 cut(s)	310, 383, 1886, 2147, 3247, 4013
SmoI	CTYRAG	6 cut(s)	310, 383, 1886, 2147, 3247, 4013
SphI	GCATGC	3 cut(s)	1096, 1205, 2810
SspDI	GGCGCC	1 cut(s)	2756
SspI	AATATT	1 cut(s)	1321
SspMI	CTAG	5 cut(s)	60, 1212, 1832, 2163, 3791
SstI	GAGCTC	2 cut(s)	2541, 3256
StyI	CCWWGG	1 cut(s)	3790
SwaI	ATTTAAAT	1 cut(s)	1539
TaiI	ACGT	5 cut(s)	371, 1082, 3222, 3457, 3936
TaqII	GACCGA	1 cut(s)	948
TatI	WGTACW	2 cut(s)	3545, 3655
TauI	GCSGC	2 cut(s)	240, 3469
Tru1I	TTAA	8 cut(s)	591, 1166, 1538, 1695, 2609, 3248, 3267, 3858
Tru9I	TTAA	8 cut(s)	591, 1166, 1538, 1695, 2609, 3248, 3267, 3858
TseFI	GTSAC	5 cut(s)	80, 689, 822, 2371, 3799
Tsp45I	GTSAC	5 cut(s)	80, 689, 822, 2371, 3799
TspGWI	ACGGA	2 cut(s)	739, 3299
Van91I	CCANNNNNTGG	1 cut(s)	2357
Vha464I	CTTAAG	1 cut(s)	3247
VneI	GTGCAC	1 cut(s)	2274
VpaK11BI	GGWCC	5 cut(s)	358, 929, 2117, 2347, 3625
XagI	CCTNNNNNAGG	1 cut(s)	1386
XapI	RAATTY	5 cut(s)	499, 859, 1352, 1598, 2529
XbaI	TCTAGA	1 cut(s)	1831
XceI	RCATGY	4 cut(s)	1096, 1205, 2810, 3473
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1792
XmaJI	CCTAGG	1 cut(s)	3790
XmiI	GTMKAC	2 cut(s)	721, 3097
XmnI	GAANNNNTTC	3 cut(s)	20, 2187, 2907
XspI	CTAG	5 cut(s)	60, 1212, 1832, 2163, 3791
Zsp2I	ATGCAT	1 cut(s)	2655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.