Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0012193

Physical Location & Seq

Genomic Coordinates Forward (+)

61723704 .. 61729994

Gene Structure 6291 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG444400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4101 bp

ATGGCGATTGAGAAGAATAACTTCAAGGTGTCGAGGTTGGATTCGGAGGTCTCTCCTGGTAGTAGGAAGAGTGTCTCCAGTGACGATGATGAGCTTCAGCAGCGTAGCTCTGCTGCTGAATCTGATGATGATGATGAGTTTGATGATGCTGACTCCGGGGCCGGGTCGGATGACTTTGATTTGTTGGAATTGGGGGAAACCGGTGTCGAGTATTGCCAGGTTGGGAATCAGACTTGCGGCATTCCTTTCGAGCTGTATGATCTTCCGAGTCTGGAGGATATATTGTCAGTGGATGTGTGGAATGAGTGCTTGAGTGAGGAGGAGCAGTTCGGCCTTACTAAGTATCTGCCTGACATGGACCAGGAGACGTTTATGATTACCCTCAAGGAGCTTTTTACGGGGTGTAACTTCCATTTTGGGAGCCCTGTTACCAGGCTGTTTGATAAGTTGAAGGGAGGTTTGTGCGAGCCAAGAGTTGCCCTTTACCGGGAGGGTTTGAATTTCTTTCAGCACCGGCGACACTACAATCTTTTGAGGAAGCATCAGAACACTATGGTTCGTAATCTCTGTCAGATAAGGGATGCTTGGCTTAATTGCAAGGGATACAGTATTGAGGAGAGGCTCCGGGTGTTGAATATAATGAGAATCCAGAAGAGTTTGATGTCTGAGAAGATGGAAGATATGGCCAGTGACTCATCAGAAAGAGATTCAGGTGAAGGTCTACGGAGTAACAAGATCAAGGACAGGAAAGTTGCACAGCAAATCAGTCGTCATTCTCCGTATGGGGTGGGCTCGAATATGGATTTTGCATCAAAAGGGCGGTCACCGTCTTTGGAAGTGGCCAAATATGGGAAGCAGAATTCCAAAGGCATACTGAAGTTGGGTGGGTCAAAGACTCCTTTAGAAAAAGAGCCAGTGAGTTATCCTGGACCATACAGTTCCGCAGCTGTTCTTCCTCGGTCGAATAAGCTTGGGGCGTATGATTCTGGGGCAGCTCTCAGAATGAGGGATCAGATGATAAGTAGTGATGATGCTGAAGAAACAACATATGGAATTGATGTTCAACGGGATCGATTTGCGTCTCGTGGTAGCATGCCTGACAAGTCCGGTCTTCTGAAAGTGGGAAAGAATTTTATAAGAGGTAATGATGTAATCGCTGACAGTTTAATGGGTCTGCCTCTCTCTACAAAAAATGATGTGCATGCTTATGCTAGGAACCGGGATGCAAACCTGTTGTCGGAGGCAAAGGTATTGACAGCAAAACCTCCTAACATGAGGGCTCCATATGATTTTGGTATAAAGACCAAGTATCCAGGAAATATTCAGCAATATGCAGTGGGCGATCAGATGAAATTCTTGAAAGGCAGACTTCCACAGGCACCTTTCAGAGGAGATCGATATGATTCATCCGATCATGCTGATCTATTCTGGAATAACAGGAGTGAAGGGGAGGCTTTCGCTATGGAATCCCCCTTTCGAGCAGATGATTGGAGTCTTAGGAGCAAGAAATGGAAGACTGGGAGGGAGTCTCCTGATTTAAATTACAAGTCCTATAGAGCCTCCCCGCCACAGATGAATGATAGATTTTTATCTTCTGAATTTAGAGCAAAACCATTGCAAGGGAAGATAAGAGGGAATAAACTACATAATGGAGGATCAGACATGGTGGCATTGAAAGGTAATAGAATGTTTGTTAAAAATGAAGAAACAGAATCAGACTCATCAGATCAATTTGAGGATGATGAGGATAGCAACCCTTTGCTGAGGAGCAAGTTGGCTTATCCCAGTGGCGTTATGGAACCTTCTCCATCTTCATTGTTGATGCCCAGTCTAGATGCAAAAAGAGCCAAATTTGCGCAGAAAGAGGTGAAGAATATGCAGGCCCTTGAGGGGATCAATTACTCCTCCAAAAAGATGGGTGGTTTTGTTGATCAAGGAAATATGCGTAGTTTAGATAACTATTCTTCCAAAGGAAAACAGAAGGGTAAGATGGGAGACAGTAGTCCCTTGCATTTGGAAGGGCGCTATTTCCCCAGCTTGGATAAACTTGACGACAATGATGATGATGAACTGAAGCCACTCTACAAATTGGGCAAGAATGCAAAAGTTCAAGGGGGACCTGGTGAAAGGTTGCATATACCCTCACTCAAGACTTATACTGCTAGTGGAAAGCAGAAACGAGAAGTTGTTCATGACCATTCTGTTTCACAGTCTCAATACTTTGTTGATGAGGAAGATGGTAGTGCACAGGGTAGATCCAGAAACAAAGGCCAGAATGTCGAAGGATATTTGAGGGATCACCGTGAAAACATTGAGGTCCCAATACTTGGCTGCAGTTTGGTGACAAAGAAGCGGAAAGGGAAAGAGGATGCCATGGATATGAGTAGAGGAGATGAAGATCTACTGTCCAATCATTTGCAACGCAGTGCTGAGTCCCATTCTTTGAAGAAAAAGGTAAAGAGGAAGATGGAGACAGACACTGGTAGTTCAGATATGGAAATTTCTGAGCTCCCAGTTACAGAAATGGGAGCAACAGATATGGAGCTGGAAACCAAGCCCCAGAAAAAGCCATTTATTTTAATTACACCCACTGTTCATACTGGTTTCTCATTCTCGATTATGCATCTTCTTTCAGCAGTTCGCTTGGCAATGATTACTCAACTTTCGGAAGATACTTTAGATGTTGGGGAACCTATAGATGAGAAGAACAAAAGTCAGGAAGGTGGTGCCAATGGCGTTATTACTCATGAAAAGGTGGATGCAAATAACGGTGAGCATGCTGGAGAAGGGAGTACGCCTTTTGTAACAGTTCAGGAGATTGTGAATCGTGTTAGATCGAATCCAGGAGATCCTTGCATTCTTGAAACACAAGAACCACTTCAGGATCTGGTGAGAGGAGTTCTGAAGATTTTTTCATCAAAAACAGCACCTTTAGGGGCAAAGGGTTGGAAGCCATTAGCTGCTTATGAAAAAGCTACAAAAAGTTGGTCATGGACTGGCCCAGTCTCTCATAGTTCATCAGATAATGAGACCATTGAGGAGGTAACATCTCCTGAAGCATGGGGTCTTCCTCACAAAATGCTTGTCAAGTTGGTTGATTCGTTTGCTAATTGGCTCAAATGTGGGCAAGAGACCCTCCAACAAATAGGAAGTCTTCCTGCACCGCCTTTGGAGTTGATGCAGCCCAACGTAGATGAGAAGGAGAGGTTTCGGGACTTAAGAGCTCAAAAGAGTCTTAATACCATCAATCCAAGTTCTGAAGAAGTGAAAGCTTATTTCCGTAAGGAGGAACTTCTTAGGTATTCAGTACCTGACAGGGCCTTCTCTTACACCGCAGCTGATGGTAAAAAATCTATTGTTGCCCCATTGAGAAGATGCGGTGGTAAGCCAACATCAAAAGCCCGAGATCACTTTATGCTGAAACGTGATCGGCCGCCGCATGTTACGATCCTTTGTCTTGTGAGAGACGCAGCTGCCAGATTGCCTGGAAGTATTGGAACCAGAGCAGATGTATGTACTTTGATAAGAGATTCTCAGTATATTGTTGAAGATGTGTCTGATACACAAGTGAATCAAGTTGTTAGTGGAGCCCTGGACCGCTTGCATTATGAGCGTGATCCTTGTGTACAGTTTGATGGGGAAAGAAAATTATGGGTCTATTTACATAGAGAAAGAGAAGAAGAAGATTTTGAGGACGATGGTACTTCATCTACAAAGAAATGGAAGAGGCAGAAAAAGGATGCTGCTGATCAAGCTGACCTAGGAGGAGTGACAGTGGCTTATCATGGATCTGAGGAACAATCTGGATATGATTTGTGCTCCGATCTTAATGCAGACCCATCATTTTTGGATGATGACAAGGGAGTGGAACTTGAATACGATGATGTGAGGCAAGATGCAGACGTTGATGTTGATACCAATCAGGGGTCTGAACAAGACGAAATGCGTCAAGACAATCCACTGGTTTGGGAGGGTCTTGACTTAAATCCTATGCGTGAAAGTAAATTGCTTTGTCAGGAAAACTCCACAAATGAAGATTTTGATGATGAAACGTTTGGCAGAGAAAGGACCGTTGGTCTCTTGAGTGCAAGCTTACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0002020 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0019899 GO:0031011 GO:0031974 GO:0031981 GO:0032991 GO:0033202 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0070603 GO:0097346 GO:1902494 GO:1904949

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1366

Amino Acids

153.01

Weight (kDa)

5.38

Isoelectric Point (pI)

44.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_2nd_NFRKB	PF25793	1090 - 1228	7e-65	NFRKB second winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0012193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45830
fragaria_vesca	FvH4_3g34430 FvH4_3g34430
malus_domestica	MD03G1106200.v1.1 MD11G1118300.v1.1
prunus_persica	Prupe.6G088700_v2.0.a1
pyrus_communis	pycom03g08520 pycom11g09930
rosa_chinensis	RchiOBHm_Chr5g0062031
rosa_laevigata	RLG00000035527
rosa_multiflora	Rmu_sc0002296.1_g000008 Rmu_sc0017669.1_g000005
rosa_roxburghii	Rroxscaffold_1G00018660
rosa_rugosa	Rorug05G0345700
rosa_samantha	Rh5AG407000 Rh5BG420300 Rh5CG444400 Rh5DG435700
rosa_wichuraiana	Rw5G038350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1136
Acc16I	TGCGCA	1 cut(s)	1857
AccB1I	GGYRCC	2 cut(s)	1378, 2726
AccB7I	CCANNNNNTGG	1 cut(s)	2327
AccI	GTMKAC	1 cut(s)	721
AclI	AACGTT	1 cut(s)	4052
AcoI	YGGCCR	3 cut(s)	684, 840, 3431
AcsI	RAATTY	7 cut(s)	499, 859, 1129, 1352, 1598, 1850, 2499
AcuI	CTGAAG	8 cut(s)	80, 896, 1056, 2093, 2864, 2924, 3075, 3279
AfaI	GTAC	5 cut(s)	2794, 3309, 3517, 3627, 3703
AflII	CTTAAG	1 cut(s)	3217
AgeI	ACCGGT	1 cut(s)	200
AhdI	GACNNNNNGTC	1 cut(s)	4074
AjuI	GAANNNNNNNTTGG	2 cut(s)	4056, 4088
AloI	GAACNNNNNNTCC	4 cut(s)	311, 343, 1044, 1076
Alw21I	GWGCWC	4 cut(s)	2248, 2511, 3226, 3821
Alw44I	GTGCAC	1 cut(s)	2244
AlwNI	CAGNNNCTG	3 cut(s)	2480, 2887, 3311
Ama87I	CYCGRG	1 cut(s)	3402
AoxI	GGCC	9 cut(s)	159, 331, 684, 840, 1881, 2269, 2998, 3318, 3431
ApaLI	GTGCAC	1 cut(s)	2244
ApoI	RAATTY	7 cut(s)	499, 859, 1129, 1352, 1598, 1850, 2499
AsiGI	ACCGGT	1 cut(s)	200
Asp700I	GAANNNNTTC	3 cut(s)	20, 2187, 2877
AspA2I	CCTAGG	1 cut(s)	3760
AspLEI	GCGC	2 cut(s)	1858, 2025
AsuC2I	CCSGG	5 cut(s)	157, 163, 488, 626, 1220
AsuHPI	GGTGA	8 cut(s)	725, 816, 1879, 2135, 2291, 2353, 2783, 2902
AvaI	CYCGRG	1 cut(s)	3402
AvaII	GGWCC	6 cut(s)	358, 929, 2117, 2317, 3595, 4068
AvrII	CCTAGG	1 cut(s)	3760
BaeGI	GKGCMC	1 cut(s)	2248
BaeI	ACNNNNGTAYC	2 cut(s)	3552, 3585
BalI	TGGCCA	2 cut(s)	686, 842
BanI	GGYRCC	2 cut(s)	1378, 2726
BanII	GRGCYC	6 cut(s)	425, 794, 1282, 2511, 3226, 3592
BarI	GAAGNNNNNNTAC	2 cut(s)	2066, 2098
BauI	CACGAG	1 cut(s)	1083
BbsI	GAAGAC	4 cut(s)	1103, 1520, 3059, 3146
Bbv12I	GWGCWC	4 cut(s)	2248, 2511, 3226, 3821
BbvCI	CCTCAGC	1 cut(s)	1763
BcgI	CGANNNNNNTGC	6 cut(s)	229, 263, 749, 783, 3812, 3846
BciVI	GTATCC	2 cut(s)	596, 1320
BclI	TGATCA	2 cut(s)	1930, 3748
BcnI	CCSGG	5 cut(s)	157, 163, 488, 626, 1220
BfaI	CTAG	4 cut(s)	1212, 1832, 2163, 3761
BfmI	CTRYAG	3 cut(s)	1552, 2332, 2694
BfoI	RGCGCY	1 cut(s)	2026
BfrI	CTTAAG	1 cut(s)	3217
BfuI	GTATCC	2 cut(s)	596, 1320
BglII	AGATCT	1 cut(s)	2398
BlnI	CCTAGG	1 cut(s)	3760
Bme18I	GGWCC	6 cut(s)	358, 929, 2117, 2317, 3595, 4068
BmeRI	GACNNNNNGTC	1 cut(s)	4074
BmeT110I	CYCGRG	1 cut(s)	3402
BmrI	ACTGGG	5 cut(s)	1527, 1779, 1821, 2507, 2996
BmuI	ACTGGG	5 cut(s)	1527, 1779, 1821, 2507, 2996
BoxI	GACNNNNGTC	1 cut(s)	2001
BpiI	GAAGAC	4 cut(s)	1103, 1520, 3059, 3146
BplI	GAGNNNNNCTC	2 cut(s)	1513, 1545
BpmI	CTGGAG	3 cut(s)	61, 293, 2802
Bpu10I	CCTNAGC	1 cut(s)	1763
BpuEI	CTTGAG	4 cut(s)	331, 368, 1907, 2132
BpuMI	CCSGG	5 cut(s)	157, 163, 488, 626, 1220
Bsa29I	ATCGAT	2 cut(s)	1072, 1396
BsaBI	GATNNNNATC	3 cut(s)	1588, 2397, 3918
BsaI	GGTCTC	4 cut(s)	55, 3023, 3125, 4082
BsaJI	CCNNGG	5 cut(s)	156, 956, 2373, 3591, 3760
BsaWI	WCCGGW	2 cut(s)	200, 1106
BsaXI	ACNNNNNCTCC	4 cut(s)	311, 341, 3759, 3789
Bse118I	RCCGGY	2 cut(s)	200, 513
Bse3DI	GCAATG	2 cut(s)	1613, 2655
Bse8I	GATNNNNATC	3 cut(s)	1588, 2397, 3918
BseCI	ATCGAT	2 cut(s)	1072, 1396
BseDI	CCNNGG	5 cut(s)	156, 956, 2373, 3591, 3760
BseJI	GATNNNNATC	3 cut(s)	1588, 2397, 3918
BseMI	GCAATG	2 cut(s)	1613, 2655
BseMII	CTCAG	7 cut(s)	657, 1012, 1754, 2421, 2496, 3548, 3783
BseSI	GKGCMC	1 cut(s)	2248
BseX3I	CGGCCG	1 cut(s)	3431
BseYI	CCCAGC	1 cut(s)	2033
BsgI	GTGCAG	1 cut(s)	3144
Bsh1285I	CGRYCG	2 cut(s)	962, 3434
BshFI	GGCC	9 cut(s)	161, 333, 686, 842, 1883, 2271, 3000, 3320, 3433
BshNI	GGYRCC	2 cut(s)	1378, 2726
BshTI	ACCGGT	1 cut(s)	200
BshVI	ATCGAT	2 cut(s)	1072, 1396
BsiEI	CGRYCG	2 cut(s)	962, 3434
BsiHKAI	GWGCWC	4 cut(s)	2248, 2511, 3226, 3821
BsiHKCI	CYCGRG	1 cut(s)	3402
BsiSI	CCGG	8 cut(s)	156, 162, 201, 487, 514, 625, 1107, 1219
BslFI	GGGAC	5 cut(s)	1989, 2130, 2303, 2419, 3227
BsmBI	CGTCTC	3 cut(s)	359, 1086, 3459
BsmFI	GGGAC	5 cut(s)	1989, 2130, 2303, 2419, 3227
BsmI	GAATGC	3 cut(s)	240, 2104, 2856
BsnI	GGCC	9 cut(s)	161, 333, 686, 842, 1883, 2271, 3000, 3320, 3433
Bso31I	GGTCTC	4 cut(s)	55, 3023, 3125, 4082
BsoBI	CYCGRG	1 cut(s)	3402
Bsp1286I	GDGCHC	8 cut(s)	425, 794, 1282, 2248, 2511, 3226, 3592, 3821
Bsp1407I	TGTACA	1 cut(s)	3625
Bsp19I	CCATGG	1 cut(s)	2373
BspANI	GGCC	9 cut(s)	161, 333, 686, 842, 1883, 2271, 3000, 3320, 3433
BspCNI	CTCAG	7 cut(s)	658, 1011, 1755, 2422, 2497, 3547, 3784
BspDI	ATCGAT	2 cut(s)	1072, 1396
BspHI	TCATGA	2 cut(s)	2191, 2746
BspMAI	CTGCAG	1 cut(s)	2336
BspT107I	GGYRCC	2 cut(s)	1378, 2726
BspTI	CTTAAG	1 cut(s)	3217
BspTNI	GGTCTC	4 cut(s)	55, 3023, 3125, 4082
BsrDI	GCAATG	2 cut(s)	1613, 2655
BsrFI	RCCGGY	2 cut(s)	200, 513
BsrGI	TGTACA	1 cut(s)	3625
BssAI	RCCGGY	2 cut(s)	200, 513
BssECI	CCNNGG	5 cut(s)	156, 956, 2373, 3591, 3760
BssSI	CACGAG	1 cut(s)	1083
BssT1I	CCWWGG	2 cut(s)	2373, 3760
Bst2BI	CACGAG	1 cut(s)	1083
Bst6I	CTCTTC	3 cut(s)	62, 647, 3719
BstAFI	CTTAAG	1 cut(s)	3217
BstAUI	TGTACA	1 cut(s)	3625
BstC8I	GCNNGC	7 cut(s)	467, 1094, 1203, 1881, 2778, 3602, 4090
BstDSI	CCRYGG	1 cut(s)	2373
BstEII	GGTNACC	1 cut(s)	822
BstENI	CCTNNNNNAGG	1 cut(s)	1386
BstH2I	RGCGCY	1 cut(s)	2026
BstHHI	GCGC	2 cut(s)	1858, 2025
BstMCI	CGRYCG	2 cut(s)	962, 3434
BstMWI	GCNNNNNNNGC	5 cut(s)	100, 1844, 1853, 3610, 3752
BstNSI	RCATGY	4 cut(s)	1096, 1205, 2780, 3443
BstPAI	GACNNNNGTC	1 cut(s)	2001
BstPI	GGTNACC	1 cut(s)	822
BstSFI	CTRYAG	3 cut(s)	1552, 2332, 2694
BstSLI	GKGCMC	1 cut(s)	2248
BstV2I	GAAGAC	4 cut(s)	1103, 1520, 3059, 3146
BstX2I	RGATCY	5 cut(s)	2255, 2398, 2848, 2884, 3788
BstXI	CCANNNNNNTGG	2 cut(s)	1915, 3966
BstYI	RGATCY	5 cut(s)	2255, 2398, 2848, 2884, 3788
BstZI	CGGCCG	1 cut(s)	3431
Bsu15I	ATCGAT	2 cut(s)	1072, 1396
BsuI	GTATCC	2 cut(s)	596, 1320
BsuRI	GGCC	9 cut(s)	161, 333, 686, 842, 1883, 2271, 3000, 3320, 3433
BsuTUI	ATCGAT	2 cut(s)	1072, 1396
BtgI	CCRYGG	1 cut(s)	2373
BtsI	GCAGTG	2 cut(s)	1341, 2431
Cac8I	GCNNGC	7 cut(s)	467, 1094, 1203, 1881, 2778, 3602, 4090
CaiI	CAGNNNCTG	3 cut(s)	2480, 2887, 3311
CciI	TCATGA	2 cut(s)	2191, 2746
CfoI	GCGC	2 cut(s)	1858, 2025
Cfr10I	RCCGGY	2 cut(s)	200, 513
ClaI	ATCGAT	2 cut(s)	1072, 1396
CseI	GACGC	3 cut(s)	1068, 3476, 3935
CsiI	ACCWGGT	1 cut(s)	2119
Csp6I	GTAC	5 cut(s)	2793, 3308, 3516, 3626, 3702
CspAI	ACCGGT	1 cut(s)	200
CviQI	GTAC	5 cut(s)	2793, 3308, 3516, 3626, 3702
DraI	TTTAAA	1 cut(s)	1539
DriI	GACNNNNNGTC	1 cut(s)	4074
EaeI	YGGCCR	3 cut(s)	684, 840, 3431
EagI	CGGCCG	1 cut(s)	3431
Eam1104I	CTCTTC	3 cut(s)	62, 647, 3719
Eam1105I	GACNNNNNGTC	1 cut(s)	4074
EarI	CTCTTC	3 cut(s)	62, 647, 3719
Ecl136II	GAGCTC	2 cut(s)	2509, 3224
EclXI	CGGCCG	1 cut(s)	3431
Eco130I	CCWWGG	2 cut(s)	2373, 3760
Eco24I	GRGCYC	6 cut(s)	425, 794, 1282, 2511, 3226, 3592
Eco31I	GGTCTC	4 cut(s)	55, 3023, 3125, 4082
Eco47I	GGWCC	6 cut(s)	358, 929, 2117, 2317, 3595, 4068
Eco52I	CGGCCG	1 cut(s)	3431
Eco53kI	GAGCTC	2 cut(s)	2509, 3224
Eco57I	CTGAAG	8 cut(s)	80, 896, 1056, 2093, 2864, 2924, 3075, 3279
Eco88I	CYCGRG	1 cut(s)	3402
Eco91I	GGTNACC	1 cut(s)	822
EcoICRI	GAGCTC	2 cut(s)	2509, 3224
EcoNI	CCTNNNNNAGG	1 cut(s)	1386
EcoO109I	RGGNCCY	4 cut(s)	1882, 2117, 2317, 3318
EcoO65I	GGTNACC	1 cut(s)	822
EcoRI	GAATTC	1 cut(s)	859
EcoT14I	CCWWGG	2 cut(s)	2373, 3760
EcoT22I	ATGCAT	1 cut(s)	2625
EcoT38I	GRGCYC	6 cut(s)	425, 794, 1282, 2511, 3226, 3592
ErhI	CCWWGG	2 cut(s)	2373, 3760
Esp3I	CGTCTC	3 cut(s)	359, 1086, 3459
FalI	AAGNNNNNCTT	7 cut(s)	37, 568, 600, 1353, 1385, 2862, 2894
FaqI	GGGAC	5 cut(s)	1989, 2130, 2303, 2419, 3227
FauI	CCCGC	1 cut(s)	1572
FauNDI	CATATG	2 cut(s)	1048, 1285
FbaI	TGATCA	2 cut(s)	1930, 3748
FblI	GTMKAC	1 cut(s)	721
FriOI	GRGCYC	6 cut(s)	425, 794, 1282, 2511, 3226, 3592
FspBI	CTAG	4 cut(s)	1212, 1832, 2163, 3761
FspI	TGCGCA	1 cut(s)	1857
GlaI	GCGC	2 cut(s)	1857, 2024
GsaI	CCCAGC	1 cut(s)	2037
GsuI	CTGGAG	3 cut(s)	61, 293, 2802
HaeII	RGCGCY	1 cut(s)	2026
HaeIII	GGCC	9 cut(s)	161, 333, 686, 842, 1883, 2271, 3000, 3320, 3433
HapII	CCGG	8 cut(s)	156, 162, 201, 487, 514, 625, 1107, 1219
HgaI	GACGC	3 cut(s)	1068, 3476, 3935
HhaI	GCGC	2 cut(s)	1858, 2025
Hin6I	GCGC	2 cut(s)	1856, 2023
HinP1I	GCGC	2 cut(s)	1856, 2023
HindIII	AAGCTT	3 cut(s)	968, 3270, 4090
HpaII	CCGG	8 cut(s)	156, 162, 201, 487, 514, 625, 1107, 1219
HphI	GGTGA	8 cut(s)	725, 816, 1879, 2135, 2291, 2353, 2783, 2902
Hpy166II	GTNNAC	3 cut(s)	722, 2246, 3626
Hpy8I	GTNNAC	3 cut(s)	722, 2246, 3626
HpyCH4IV	ACGT	5 cut(s)	368, 3189, 3424, 3903, 4052
HpyF10VI	GCNNNNNNNGC	5 cut(s)	100, 1844, 1853, 3610, 3752
HpySE526I	ACGT	5 cut(s)	368, 3189, 3424, 3903, 4052
HspAI	GCGC	2 cut(s)	1856, 2023
Ksp22I	TGATCA	2 cut(s)	1930, 3748
MabI	ACCWGGT	1 cut(s)	2119
MaeI	CTAG	4 cut(s)	1212, 1832, 2163, 3761
MaeII	ACGT	5 cut(s)	368, 3189, 3424, 3903, 4052
MflI	RGATCY	5 cut(s)	2255, 2398, 2848, 2884, 3788
MhlI	GDGCHC	8 cut(s)	425, 794, 1282, 2248, 2511, 3226, 3592, 3821
MlsI	TGGCCA	2 cut(s)	686, 842
MluNI	TGGCCA	2 cut(s)	686, 842
MlyI	GAGTC	9 cut(s)	146, 277, 686, 889, 1501, 1535, 1712, 2441, 3241
MmeI	TCCRAC	6 cut(s)	18, 147, 165, 1218, 2927, 3163
Mox20I	TGGCCA	2 cut(s)	686, 842
Mph1103I	ATGCAT	1 cut(s)	2625
MroXI	GAANNNNTTC	3 cut(s)	20, 2187, 2877
MscI	TGGCCA	2 cut(s)	686, 842
MseI	TTAA	9 cut(s)	591, 1166, 1538, 1695, 2579, 3218, 3237, 3828, 3983
MslI	CAYNNNNRTG	2 cut(s)	1206, 2378
Msp20I	TGGCCA	2 cut(s)	686, 842
MspA1I	CMGCKG	3 cut(s)	947, 3338, 3473
MspCI	CTTAAG	1 cut(s)	3217
MspI	CCGG	8 cut(s)	156, 162, 201, 487, 514, 625, 1107, 1219
Mva1269I	GAATGC	3 cut(s)	240, 2104, 2856
MwoI	GCNNNNNNNGC	5 cut(s)	100, 1844, 1853, 3610, 3752
NciI	CCSGG	5 cut(s)	157, 163, 488, 626, 1220
NcoI	CCATGG	1 cut(s)	2373
NdeI	CATATG	2 cut(s)	1048, 1285
NmuCI	GTSAC	5 cut(s)	80, 689, 822, 2341, 3769
NsbI	TGCGCA	1 cut(s)	1857
NsiI	ATGCAT	1 cut(s)	2625
NspI	RCATGY	4 cut(s)	1096, 1205, 2780, 3443
PaeI	GCATGC	3 cut(s)	1096, 1205, 2780
PagI	TCATGA	2 cut(s)	2191, 2746
PctI	GAATGC	3 cut(s)	240, 2104, 2856
PdmI	GAANNNNTTC	3 cut(s)	20, 2187, 2877
PflMI	CCANNNNNTGG	1 cut(s)	2327
PfoI	TCCNGGA	3 cut(s)	925, 1312, 2842
PinAI	ACCGGT	1 cut(s)	200
PleI	GAGTC	9 cut(s)	146, 276, 686, 889, 1500, 1534, 1712, 2440, 3240
PpsI	GAGTC	9 cut(s)	146, 276, 686, 889, 1500, 1534, 1712, 2440, 3240
PpuMI	RGGWCCY	2 cut(s)	2117, 2317
PshAI	GACNNNNGTC	1 cut(s)	2001
PsiI	TTATAA	1 cut(s)	1136
Psp124BI	GAGCTC	2 cut(s)	2511, 3226
Psp1406I	AACGTT	1 cut(s)	4052
Psp5II	RGGWCCY	2 cut(s)	2117, 2317
PspEI	GGTNACC	1 cut(s)	822
PspFI	CCCAGC	1 cut(s)	2033
PspPPI	RGGWCCY	2 cut(s)	2117, 2317
PstI	CTGCAG	1 cut(s)	2336
PstNI	CAGNNNCTG	3 cut(s)	2480, 2887, 3311
PsuI	RGATCY	5 cut(s)	2255, 2398, 2848, 2884, 3788
PvuII	CAGCTG	3 cut(s)	947, 3338, 3473
RsaI	GTAC	5 cut(s)	2794, 3309, 3517, 3627, 3703
RsaNI	GTAC	5 cut(s)	2793, 3308, 3516, 3626, 3702
RseI	CAYNNNNRTG	2 cut(s)	1206, 2378
SacI	GAGCTC	2 cut(s)	2511, 3226
SaqAI	TTAA	9 cut(s)	591, 1166, 1538, 1695, 2579, 3218, 3237, 3828, 3983
SchI	GAGTC	9 cut(s)	146, 277, 686, 889, 1501, 1535, 1712, 2441, 3241
SduI	GDGCHC	8 cut(s)	425, 794, 1282, 2248, 2511, 3226, 3592, 3821
SexAI	ACCWGGT	1 cut(s)	2119
SfcI	CTRYAG	3 cut(s)	1552, 2332, 2694
SgrAI	CRCCGGYG	1 cut(s)	513
SinI	GGWCC	6 cut(s)	358, 929, 2117, 2317, 3595, 4068
SmiI	ATTTAAAT	1 cut(s)	1539
SmiMI	CAYNNNNRTG	2 cut(s)	1206, 2378
SmlI	CTYRAG	6 cut(s)	310, 383, 1886, 2147, 3217, 4081
SmoI	CTYRAG	6 cut(s)	310, 383, 1886, 2147, 3217, 4081
SphI	GCATGC	3 cut(s)	1096, 1205, 2780
SspI	AATATT	1 cut(s)	1321
SspMI	CTAG	4 cut(s)	1212, 1832, 2163, 3761
SstI	GAGCTC	2 cut(s)	2511, 3226
StyI	CCWWGG	2 cut(s)	2373, 3760
SwaI	ATTTAAAT	1 cut(s)	1539
TaiI	ACGT	5 cut(s)	371, 3192, 3427, 3906, 4055
TaqII	GACCGA	1 cut(s)	948
TatI	WGTACW	2 cut(s)	3515, 3625
TauI	GCSGC	3 cut(s)	240, 3436, 3439
Tru1I	TTAA	9 cut(s)	591, 1166, 1538, 1695, 2579, 3218, 3237, 3828, 3983
Tru9I	TTAA	9 cut(s)	591, 1166, 1538, 1695, 2579, 3218, 3237, 3828, 3983
TseFI	GTSAC	5 cut(s)	80, 689, 822, 2341, 3769
Tsp45I	GTSAC	5 cut(s)	80, 689, 822, 2341, 3769
TspGWI	ACGGA	3 cut(s)	739, 768, 3269
Van91I	CCANNNNNTGG	1 cut(s)	2327
Vha464I	CTTAAG	1 cut(s)	3217
VneI	GTGCAC	1 cut(s)	2244
VpaK11BI	GGWCC	6 cut(s)	358, 929, 2117, 2317, 3595, 4068
XagI	CCTNNNNNAGG	1 cut(s)	1386
XapI	RAATTY	7 cut(s)	499, 859, 1129, 1352, 1598, 1850, 2499
XbaI	TCTAGA	1 cut(s)	1831
XceI	RCATGY	4 cut(s)	1096, 1205, 2780, 3443
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1792
XmaJI	CCTAGG	1 cut(s)	3760
XmiI	GTMKAC	1 cut(s)	721
XmnI	GAANNNNTTC	3 cut(s)	20, 2187, 2877
XspI	CTAG	4 cut(s)	1212, 1832, 2163, 3761
Zsp2I	ATGCAT	1 cut(s)	2625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.