MD03G1110300.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010861
Physical Location & Seq
Forward (+)
9608146 .. 9612630
4485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1110300.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGCAACTTATTACATGTCAGCAACTCAGAGAGAGTACACATCACATTTCTCACCCTCACTCAAAACCTAAAAACCCTTATAAAACTAACACCACCGCTGGACCTCATCAAAACTTCAAACAAACTTCAGAAGTACAATCAAAGCAGAGCTGCAGCGGAGTAGAAGTAGAGAAAACAAAACCCAGAAGAAAAAAAAGATTAAAAAAAATCATGAGGACTAGTAACCACTTGATTGGACTACTAAATTTCATAACTTTCCTCCTCTCGATCCCCATCCTGGGCGGAGGAATCTGGCTGAGCAGCCGCGCCAACAACACCGACTGCCTCAAGTTCCTCCAATGGCCGCTCATCGTCATAGGGGTCGCAATCATGGTGGTTTCTCTTGCTGGGTTCGCGGGTGCGTGCTACAGGAACACCTTTCTCATGTGGGCGTACCTATTCGTCATGTTCTTCATCATCGCCGCCCTCATCGGCTTCATCATCTTCGCCTACGCGGTCACCGACAAGGGCTCCGGCAAGCCGGTGATGAATAGAGCTTACTTGGACTACTACCTGCAGGACTATTCCGGCTGGCTGGAGGAGCGCGTGGCGGACAATAGCTACTGGGGGAAGATAGCCTCTTGTGTGAGGGACTCAAAAACCTGTAAAAAGATGGGGAGGACTATGAATGGTGTGCCTGAGACTGCTGACATGTTCTACAACAGAAAACTCAATCCTATCCAGTCTGGCTGCTGCAAGCCCCCTACAGAATGTGGCTATAGCTATGTGAACGAGACAACCTGGACTGCCGGTGGAGGATTGGTCGGGACCAATCCGGACTGCACAATGTGGAGCAATGACCAAGAGCAACTCTGCTATTCCTGCAACTCTTGCAAAGCTGGTGTCTTGGCCAGCATCAAGAAGAGCTGGAGGAAGGTCTCTGTGATCAACATTGTTGTGTTAATCATCCTAGTTATCTTCTATGTGATAGGTTGTGCAGCATTCCGAAACAACCGTCGGATGGACAACGATGAACCCTACGGCCAGGCGCGGATGACAAAATCACGACCAAGTGCTTTCCAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

356

Amino Acids

39.98

Weight (kDa)

9.49

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 75 - 326 3e-39 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 561
AccBSI CCGCTC 1 cut(s) 346
AccII CGCG 5 cut(s) 306, 395, 494, 585, 1030
AccIII TCCGGA 1 cut(s) 814
AclWI GGATC 1 cut(s) 262
AcoI YGGCCR 3 cut(s) 341, 888, 1021
AcsI RAATTY 1 cut(s) 244
AcuI CTGAAG 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 828
AfaI GTAC 3 cut(s) 37, 135, 434
AfiI CCNNNNNNNGG 3 cut(s) 277, 278, 1000
AflIII ACRYGT 2 cut(s) 14, 690
AgsI TTSAA 1 cut(s) 118
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 3 cut(s) 276, 779, 1023
AluBI AGCT 6 cut(s) 150, 536, 600, 762, 878, 906
AluI AGCT 6 cut(s) 150, 536, 600, 762, 878, 906
Alw26I GTCTC 3 cut(s) 674, 767, 922
AlwI GGATC 1 cut(s) 262
Aor13HI TCCGGA 1 cut(s) 814
AoxI GGCC 3 cut(s) 341, 888, 1021
ApeKI GCWGC 6 cut(s) 150, 153, 300, 729, 732, 977
ApoI RAATTY 1 cut(s) 244
ArsI GACNNNNNNTTYG 4 cut(s) 867, 899, 979, 1011
AspLEI GCGC 3 cut(s) 308, 585, 1030
AspS9I GGNCC 2 cut(s) 101, 807
AsuHPI GGTGA 3 cut(s) 44, 490, 535
AvaII GGWCC 2 cut(s) 101, 807
BalI TGGCCA 1 cut(s) 890
BanII GRGCYC 1 cut(s) 512
BbvI GCAGC 6 cut(s) 137, 165, 312, 716, 719, 989
BccI CCATC 3 cut(s) 281, 646, 994
BceAI ACGGC 1 cut(s) 1036
BciT130I CCWGG 3 cut(s) 278, 781, 1025
BclI TGATCA 1 cut(s) 924
BcoDI GTCTC 3 cut(s) 674, 767, 922
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 2 cut(s) 219, 950
BfmI CTRYAG 5 cut(s) 151, 406, 554, 744, 757
BfuAI ACCTGC 1 cut(s) 561
BisI GCNGC 9 cut(s) 151, 154, 301, 304, 344, 462, 730, 733, 978
BlpI GCTNAGC 1 cut(s) 296
BlsI GCNGC 9 cut(s) 152, 155, 302, 305, 345, 463, 731, 734, 979
Bme1390I CCNGG 3 cut(s) 278, 781, 1025
Bme18I GGWCC 2 cut(s) 101, 807
BmgT120I GGNCC 2 cut(s) 101, 807
BmiI GGNNCC 2 cut(s) 511, 808
BmrFI CCNGG 3 cut(s) 278, 781, 1025
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 613
BpmI CTGGAG 2 cut(s) 596, 928
Bpu1102I GCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 1 cut(s) 277
BsaWI WCCGGW 1 cut(s) 814
BsaXI ACNNNNNCTCC 6 cut(s) 150, 180, 494, 524, 786, 816
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 278, 1000
Bse118I RCCGGY 2 cut(s) 520, 788
Bse1I ACTGG 2 cut(s) 608, 721
Bse3DI GCAATG 1 cut(s) 841
Bse8I GATNNNNATC 1 cut(s) 272
BseAI TCCGGA 1 cut(s) 814
BseBI CCWGG 3 cut(s) 278, 781, 1025
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 4 cut(s) 273, 945, 1005, 1038
BseJI GATNNNNATC 1 cut(s) 272
BseLI CCNNNNNNNGG 3 cut(s) 277, 278, 1000
BseMI GCAATG 1 cut(s) 841
BseMII CTCAG 3 cut(s) 40, 287, 669
BseNI ACTGG 2 cut(s) 608, 721
BseRI GAGGAG 2 cut(s) 251, 593
BseXI GCAGC 6 cut(s) 137, 165, 312, 716, 719, 989
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 2 cut(s) 805, 996
Bsh1236I CGCG 5 cut(s) 306, 395, 494, 585, 1030
BshFI GGCC 3 cut(s) 343, 890, 1023
BsiSI CCGG 5 cut(s) 513, 521, 567, 789, 815
BslFI GGGAC 2 cut(s) 644, 820
BslI CCNNNNNNNGG 3 cut(s) 277, 278, 1000
BsmAI GTCTC 3 cut(s) 674, 767, 922
BsmFI GGGAC 2 cut(s) 644, 820
BsmI GAATGC 1 cut(s) 980
BsnI GGCC 3 cut(s) 343, 890, 1023
Bso31I GGTCTC 1 cut(s) 922
Bsp1286I GDGCHC 1 cut(s) 512
Bsp13I TCCGGA 1 cut(s) 814
Bsp143I GATC 2 cut(s) 267, 924
Bsp1720I GCTNAGC 1 cut(s) 296
BspANI GGCC 3 cut(s) 343, 890, 1023
BspCNI CTCAG 3 cut(s) 39, 288, 670
BspEI TCCGGA 1 cut(s) 814
BspFNI CGCG 5 cut(s) 306, 395, 494, 585, 1030
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 511, 808
BspMAI CTGCAG 2 cut(s) 155, 558
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 1 cut(s) 262
BspQI GCTCTTC 1 cut(s) 896
BspTNI GGTCTC 1 cut(s) 922
BsrBI CCGCTC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 841
BsrFI RCCGGY 2 cut(s) 520, 788
BsrI ACTGG 2 cut(s) 608, 721
BssAI RCCGGY 2 cut(s) 520, 788
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 2 cut(s) 267, 924
Bst2UI CCWGG 3 cut(s) 278, 781, 1025
Bst4CI ACNGT 1 cut(s) 995
Bst6I CTCTTC 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 870
BstC8I GCNNGC 5 cut(s) 403, 518, 572, 737, 892
BstDEI CTNAG 3 cut(s) 26, 296, 678
BstEII GGTNACC 1 cut(s) 496
BstF5I GGATG 4 cut(s) 273, 945, 1005, 1038
BstFNI CGCG 5 cut(s) 306, 395, 494, 585, 1030
BstHHI GCGC 3 cut(s) 308, 585, 1030
BstKTI GATC 2 cut(s) 270, 927
BstMAI GTCTC 3 cut(s) 674, 767, 922
BstMBI GATC 2 cut(s) 267, 924
BstMWI GCNNNNNNNGC 4 cut(s) 392, 580, 861, 870
BstNI CCWGG 3 cut(s) 278, 781, 1025
BstNSI RCATGY 2 cut(s) 18, 694
BstPI GGTNACC 1 cut(s) 496
BstSCI CCNGG 3 cut(s) 276, 779, 1023
BstSFI CTRYAG 5 cut(s) 151, 406, 554, 744, 757
BstUI CGCG 5 cut(s) 306, 395, 494, 585, 1030
BstV1I GCAGC 6 cut(s) 137, 165, 312, 716, 719, 989
BstXI CCANNNNNNTGG 1 cut(s) 233
BsuRI GGCC 3 cut(s) 343, 890, 1023
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 4 cut(s) 273, 945, 1005, 1038
BveI ACCTGC 1 cut(s) 561
Cac8I GCNNGC 5 cut(s) 403, 518, 572, 737, 892
CciI TCATGA 1 cut(s) 210
CfoI GCGC 3 cut(s) 308, 585, 1030
Cfr10I RCCGGY 2 cut(s) 520, 788
Cfr13I GGNCC 2 cut(s) 101, 807
Csp6I GTAC 3 cut(s) 36, 134, 433
CspCI CAANNNNNGTGG 2 cut(s) 354, 389
CviAII CATG 6 cut(s) 15, 211, 370, 424, 445, 691
CviQI GTAC 3 cut(s) 36, 134, 433
DdeI CTNAG 3 cut(s) 26, 296, 678
DpnI GATC 2 cut(s) 269, 926
DpnII GATC 2 cut(s) 267, 924
DraIII CACNNNGTG 1 cut(s) 828
EaeI YGGCCR 3 cut(s) 341, 888, 1021
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
EciI GGCGGA 2 cut(s) 297, 605
Eco24I GRGCYC 1 cut(s) 512
Eco31I GGTCTC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 101, 807
Eco57I CTGAAG 1 cut(s) 111
Eco91I GGTNACC 1 cut(s) 496
EcoO65I GGTNACC 1 cut(s) 496
EcoRII CCWGG 3 cut(s) 276, 779, 1023
EcoT38I GRGCYC 1 cut(s) 512
FaeI CATG 6 cut(s) 18, 214, 373, 427, 448, 694
FaqI GGGAC 2 cut(s) 644, 820
FatI CATG 6 cut(s) 14, 210, 369, 423, 444, 690
FauI CCCGC 1 cut(s) 388
FbaI TGATCA 1 cut(s) 924
Fnu4HI GCNGC 9 cut(s) 151, 154, 301, 304, 344, 462, 730, 733, 978
FokI GGATG 4 cut(s) 260, 932, 1012, 1045
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 9 cut(s) 151, 154, 301, 304, 344, 462, 730, 733, 978
FspBI CTAG 2 cut(s) 219, 950
GlaI GCGC 3 cut(s) 307, 584, 1029
GluI GCNGC 9 cut(s) 151, 154, 301, 304, 344, 462, 730, 733, 978
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 2 cut(s) 596, 928
HaeIII GGCC 3 cut(s) 343, 890, 1023
HapII CCGG 5 cut(s) 513, 521, 567, 789, 815
HhaI GCGC 3 cut(s) 308, 585, 1030
Hin1II CATG 6 cut(s) 18, 214, 373, 427, 448, 694
Hin6I GCGC 3 cut(s) 306, 583, 1028
HinP1I GCGC 3 cut(s) 306, 583, 1028
HinfI GANTC 2 cut(s) 288, 632
HpaII CCGG 5 cut(s) 513, 521, 567, 789, 815
HphI GGTGA 3 cut(s) 44, 490, 535
Hpy166II GTNNAC 2 cut(s) 38, 769
Hpy188I TCNGA 4 cut(s) 29, 130, 986, 999
Hpy188III TCNNGA 6 cut(s) 211, 265, 805, 815, 898, 1044
Hpy8I GTNNAC 2 cut(s) 38, 769
Hpy99I CGWCG 1 cut(s) 999
HpyAV CCTTC 1 cut(s) 907
HpyCH4III ACNGT 1 cut(s) 995
HpyCH4V TGCA 8 cut(s) 4, 153, 556, 735, 822, 864, 873, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 392, 580, 861, 870
HpyF3I CTNAG 3 cut(s) 26, 296, 678
Hsp92II CATG 6 cut(s) 18, 214, 373, 427, 448, 694
HspAI GCGC 3 cut(s) 306, 583, 1028
Kpn2I TCCGGA 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 924
Kzo9I GATC 2 cut(s) 267, 924
LguI GCTCTTC 1 cut(s) 896
LmnI GCTCC 3 cut(s) 515, 580, 831
Lsp1109I GCAGC 6 cut(s) 137, 165, 312, 716, 719, 989
LweI GCATC 1 cut(s) 903
MaeI CTAG 2 cut(s) 219, 950
MaeIII GTNAC 2 cut(s) 221, 496
MalI GATC 2 cut(s) 269, 926
MbiI CCGCTC 1 cut(s) 346
MboI GATC 2 cut(s) 267, 924
MboII GAAGA 6 cut(s) 198, 442, 475, 622, 913, 949
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 1 cut(s) 244
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 1 cut(s) 977
Mox20I TGGCCA 1 cut(s) 890
MroI TCCGGA 1 cut(s) 814
MscI TGGCCA 1 cut(s) 890
MseI TTAA 3 cut(s) 200, 941, 1066
MslI CAYNNNNRTG 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 2 cut(s) 98, 156
MspI CCGG 5 cut(s) 513, 521, 567, 789, 815
MspR9I CCNGG 3 cut(s) 278, 781, 1025
Mva1269I GAATGC 1 cut(s) 980
MvaI CCWGG 3 cut(s) 278, 781, 1025
MvnI CGCG 5 cut(s) 306, 395, 494, 585, 1030
MwoI GCNNNNNNNGC 4 cut(s) 392, 580, 861, 870
NdeII GATC 2 cut(s) 267, 924
NlaIII CATG 6 cut(s) 18, 214, 373, 427, 448, 694
NlaIV GGNNCC 2 cut(s) 511, 808
NmuCI GTSAC 1 cut(s) 496
NspI RCATGY 2 cut(s) 18, 694
PagI TCATGA 1 cut(s) 210
PciI ACATGT 2 cut(s) 14, 690
PciSI GCTCTTC 1 cut(s) 896
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PctI GAATGC 1 cut(s) 980
PfeI GAWTC 1 cut(s) 288
PkrI GCNGC 9 cut(s) 152, 155, 302, 305, 345, 463, 731, 734, 979
PleI GAGTC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 626
PscI ACATGT 2 cut(s) 14, 690
PsiI TTATAA 1 cut(s) 81
Psp6I CCWGG 3 cut(s) 276, 779, 1023
PspEI GGTNACC 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 3 cut(s) 276, 779, 1023
PspN4I GGNNCC 2 cut(s) 511, 808
PspPI GGNCC 2 cut(s) 101, 807
PstI CTGCAG 2 cut(s) 155, 558
RsaI GTAC 3 cut(s) 37, 135, 434
RsaNI GTAC 3 cut(s) 36, 134, 433
RseI CAYNNNNRTG 1 cut(s) 935
SapI GCTCTTC 1 cut(s) 896
SaqAI TTAA 3 cut(s) 200, 941, 1066
SatI GCNGC 9 cut(s) 151, 154, 301, 304, 344, 462, 730, 733, 978
Sau3AI GATC 2 cut(s) 267, 924
Sau96I GGNCC 2 cut(s) 101, 807
SbfI CCTGCAGG 1 cut(s) 558
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 3 cut(s) 278, 781, 1025
SdaI CCTGCAGG 1 cut(s) 558
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 1 cut(s) 903
SfcI CTRYAG 5 cut(s) 151, 406, 554, 744, 757
SinI GGWCC 2 cut(s) 101, 807
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SpeI ACTAGT 1 cut(s) 218
Sse8387I CCTGCAGG 1 cut(s) 558
Sse9I AATT 1 cut(s) 244
SspMI CTAG 2 cut(s) 219, 950
StyD4I CCNGG 3 cut(s) 276, 779, 1023
TaaI ACNGT 1 cut(s) 995
TaqI TCGA 1 cut(s) 266
TasI AATT 1 cut(s) 244
TatI WGTACW 2 cut(s) 35, 133
TauI GCSGC 3 cut(s) 306, 346, 464
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 3 cut(s) 200, 941, 1066
Tru9I TTAA 3 cut(s) 200, 941, 1066
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 6 cut(s) 150, 153, 300, 729, 732, 977
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 6 cut(s) 238, 442, 466, 542, 680, 1026
VpaK11BI GGWCC 2 cut(s) 101, 807
XapI RAATTY 1 cut(s) 244
XceI RCATGY 2 cut(s) 18, 694
XspI CTAG 2 cut(s) 219, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.