RLG00000035424

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010861
Physical Location & Seq
Reverse (-)
66768048 .. 66770917
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035424

Sequence Viewer

Length: 858 bp
ATGAGGTCCAGCAACAACTTGATAGGTCTCCTCAACTTCATCACCTTCCTCCTCTCAATCCCTATCCTCGGCGGTGGAATCTGGCTGAGCAGCCGAGCCAACAACACTGACTGCCTCAAGTTCCTCCAATGGCCTCTCATAGTCATAGGAGTCTCCATCATGGTCGTCTCCTTAGCCGGCTTCGCCGGAGCTTGTTACCGTAACACTTTCCTCATGTGGGTCTACCTCTTCGTCATGTTCTTCATCATAGCTGCTCTGATCGGCTTCATCATTTTCGCTTACGCCGTGACGGATAAAGGGTCCGGCAGGGCCCTCGTAAACCGGGCTTATAAGGACTACTATCTGCAGGACTACTCTGGCTGGCTGGAGGAGCGCGTAGCAAAAAATAGCTACTGGAGGAAGATTGCTTCTTGTGTGAGGGACTCTAAGGTTTGCAAGAAGATGGACAGGACTGTTAATGGTGTGCCGGAGACTGCTGACATGTTCAACAGCAGAAAGCTTAATTCTGTTGAGTCTGGCTGCTGCAAGCCCCCTACAGAGTGTGGCTATACCTATGTGAATGAGACGTTTTGGGCCACTGAAGGAGGCTTGGTGGGAACAAATCCCGACTGTTCTATGTGGAACAATGATCAACAGGAACTCTGCTATTCCTGTAACTCTTGCAAGGCTGGTGTGCTGGCCAGCATTAGGAAGAGTTGGAGAAAGGTCTCTGTCATTAACATCGTTGTATTGATCGTCCTAGTGATCTTCTATGTGATTGGCTGTGCAGCGTTCAGAAACAATCGTCGGATTGACAATGATGAACCGTATGGGGAGGCTCGGATGACAAAGGCGCAACCTAGCCCTTTCCAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

286

Amino Acids

32.06

Weight (kDa)

8.96

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 256 4.8e-39 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 375
AciI CCGC 1 cut(s) 72
AcoI YGGCCR 1 cut(s) 678
AcuI CTGAAG 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 68, 217, 687
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 1 cut(s) 487
AluBI AGCT 4 cut(s) 191, 251, 390, 499
AluI AGCT 4 cut(s) 191, 251, 390, 499
Alw26I GTCTC 6 cut(s) 32, 157, 172, 464, 557, 712
AoxI GGCC 4 cut(s) 131, 309, 573, 678
ApaI GGGCCC 1 cut(s) 313
ApeKI GCWGC 5 cut(s) 90, 251, 519, 522, 767
AspLEI GCGC 2 cut(s) 375, 835
AspS9I GGNCC 5 cut(s) 6, 300, 309, 310, 573
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 2 cut(s) 6, 300
BaeGI GKGCMC 1 cut(s) 313
BalI TGGCCA 1 cut(s) 680
BanII GRGCYC 1 cut(s) 313
BbvI GCAGC 5 cut(s) 102, 238, 506, 509, 779
BccI CCATC 2 cut(s) 164, 436
BceAI ACGGC 1 cut(s) 269
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 6 cut(s) 32, 157, 172, 464, 557, 712
BfaI CTAG 3 cut(s) 740, 840, 856
BfmI CTRYAG 2 cut(s) 344, 534
BisI GCNGC 5 cut(s) 91, 252, 520, 523, 768
BlpI GCTNAGC 1 cut(s) 86
BlsI GCNGC 5 cut(s) 92, 253, 521, 524, 769
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 2 cut(s) 6, 300
BmgT120I GGNCC 5 cut(s) 6, 300, 309, 310, 573
BmiI GGNNCC 2 cut(s) 301, 311
BmrFI CCNGG 1 cut(s) 323
BpmI CTGGAG 2 cut(s) 386, 415
Bpu10I CCTNAGC 1 cut(s) 172
Bpu1102I GCTNAGC 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 323
BsaI GGTCTC 2 cut(s) 32, 712
BsaJI CCNNGG 1 cut(s) 67
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 217, 687
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 1 cut(s) 828
BseLI CCNNNNNNNGG 3 cut(s) 68, 217, 687
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 3 cut(s) 20, 41, 383
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 5 cut(s) 102, 238, 506, 509, 779
BsgI GTGCAG 1 cut(s) 786
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 4 cut(s) 133, 311, 575, 680
BsiSI CCGG 5 cut(s) 177, 186, 303, 322, 467
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 3 cut(s) 68, 217, 687
BsmAI GTCTC 6 cut(s) 32, 157, 172, 464, 557, 712
BsmBI CGTCTC 2 cut(s) 172, 557
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 4 cut(s) 133, 311, 575, 680
Bso31I GGTCTC 2 cut(s) 32, 712
Bsp120I GGGCCC 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 4 cut(s) 258, 628, 732, 744
Bsp1720I GCTNAGC 1 cut(s) 86
BspACI CCGC 1 cut(s) 72
BspANI GGCC 4 cut(s) 133, 311, 575, 680
BspCNI CTCAG 1 cut(s) 78
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 301, 311
BspMAI CTGCAG 1 cut(s) 348
BspTNI GGTCTC 2 cut(s) 32, 712
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 4 cut(s) 258, 628, 732, 744
Bst4CI ACNGT 4 cut(s) 200, 454, 611, 807
Bst6I CTCTTC 2 cut(s) 233, 686
BstC8I GCNNGC 5 cut(s) 178, 362, 527, 678, 682
BstDEI CTNAG 3 cut(s) 86, 172, 426
BstF5I GGATG 1 cut(s) 828
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 2 cut(s) 375, 835
BstKTI GATC 4 cut(s) 261, 631, 735, 747
BstMAI GTCTC 6 cut(s) 32, 157, 172, 464, 557, 712
BstMBI GATC 4 cut(s) 258, 628, 732, 744
BstMWI GCNNNNNNNGC 2 cut(s) 182, 370
BstNSI RCATGY 1 cut(s) 484
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 2 cut(s) 344, 534
BstSLI GKGCMC 1 cut(s) 313
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 5 cut(s) 102, 238, 506, 509, 779
BsuRI GGCC 4 cut(s) 133, 311, 575, 680
BtsCI GGATG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 105, 576
Cac8I GCNNGC 5 cut(s) 178, 362, 527, 678, 682
CfoI GCGC 2 cut(s) 375, 835
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 5 cut(s) 6, 300, 309, 310, 573
CviAII CATG 4 cut(s) 160, 214, 235, 481
DdeI CTNAG 3 cut(s) 86, 172, 426
DpnI GATC 4 cut(s) 260, 630, 734, 746
DpnII GATC 4 cut(s) 258, 628, 732, 744
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 2 cut(s) 233, 686
EarI CTCTTC 2 cut(s) 233, 686
Eco24I GRGCYC 1 cut(s) 313
Eco31I GGTCTC 2 cut(s) 32, 712
Eco47I GGWCC 2 cut(s) 6, 300
Eco57I CTGAAG 1 cut(s) 600
EcoO109I RGGNCCY 2 cut(s) 309, 310
EcoT38I GRGCYC 1 cut(s) 313
Esp3I CGTCTC 2 cut(s) 172, 557
FaeI CATG 4 cut(s) 163, 217, 238, 484
FaqI GGGAC 1 cut(s) 434
FatI CATG 4 cut(s) 159, 213, 234, 480
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 5 cut(s) 91, 252, 520, 523, 768
FokI GGATG 1 cut(s) 835
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 91, 252, 520, 523, 768
FspBI CTAG 3 cut(s) 740, 840, 856
GlaI GCGC 2 cut(s) 374, 834
GluI GCNGC 5 cut(s) 91, 252, 520, 523, 768
GsuI CTGGAG 2 cut(s) 386, 415
HaeIII GGCC 4 cut(s) 133, 311, 575, 680
HapII CCGG 5 cut(s) 177, 186, 303, 322, 467
HhaI GCGC 2 cut(s) 375, 835
Hin1II CATG 4 cut(s) 163, 217, 238, 484
Hin6I GCGC 2 cut(s) 373, 833
HinP1I GCGC 2 cut(s) 373, 833
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 4 cut(s) 78, 150, 422, 512
HpaII CCGG 5 cut(s) 177, 186, 303, 322, 467
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 223, 319
Hpy188I TCNGA 4 cut(s) 258, 776, 789, 822
Hpy188III TCNNGA 1 cut(s) 605
Hpy8I GTNNAC 2 cut(s) 223, 319
Hpy99I CGWCG 1 cut(s) 789
HpyAV CCTTC 2 cut(s) 55, 575
HpyCH4III ACNGT 4 cut(s) 200, 454, 611, 807
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 5 cut(s) 346, 435, 525, 663, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 370
HpyF3I CTNAG 3 cut(s) 86, 172, 426
HpySE526I ACGT 1 cut(s) 566
Hsp92II CATG 4 cut(s) 163, 217, 238, 484
HspAI GCGC 2 cut(s) 373, 833
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 4 cut(s) 258, 628, 732, 744
LmnI GCTCC 2 cut(s) 188, 370
Lsp1109I GCAGC 5 cut(s) 102, 238, 506, 509, 779
MaeI CTAG 3 cut(s) 740, 840, 856
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 4 cut(s) 194, 200, 286, 653
MalI GATC 4 cut(s) 260, 630, 734, 746
MboI GATC 4 cut(s) 258, 628, 732, 744
MboII GAAGA 6 cut(s) 220, 232, 412, 451, 703, 739
MhlI GDGCHC 1 cut(s) 313
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 1 cut(s) 502
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 3 cut(s) 159, 416, 521
MmeI TCCRAC 2 cut(s) 677, 767
Mox20I TGGCCA 1 cut(s) 680
MroNI GCCGGC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 680
MseI TTAA 3 cut(s) 456, 501, 717
Msp20I TGGCCA 1 cut(s) 680
MspI CCGG 5 cut(s) 177, 186, 303, 322, 467
MspR9I CCNGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 2 cut(s) 182, 370
NaeI GCCGGC 1 cut(s) 178
NciI CCSGG 1 cut(s) 323
NdeII GATC 4 cut(s) 258, 628, 732, 744
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 4 cut(s) 163, 217, 238, 484
NlaIV GGNNCC 2 cut(s) 301, 311
NmeAIII GCCGAG 2 cut(s) 48, 119
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 484
PciI ACATGT 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 78
PkrI GCNGC 5 cut(s) 92, 253, 521, 524, 769
PleI GAGTC 3 cut(s) 158, 416, 520
PpsI GAGTC 3 cut(s) 158, 416, 520
PscI ACATGT 1 cut(s) 480
PsiI TTATAA 1 cut(s) 330
PspN4I GGNNCC 2 cut(s) 301, 311
PspOMI GGGCCC 1 cut(s) 309
PspPI GGNCC 5 cut(s) 6, 300, 309, 310, 573
PstI CTGCAG 1 cut(s) 348
SaqAI TTAA 3 cut(s) 456, 501, 717
SatI GCNGC 5 cut(s) 91, 252, 520, 523, 768
Sau3AI GATC 4 cut(s) 258, 628, 732, 744
Sau96I GGNCC 5 cut(s) 6, 300, 309, 310, 573
SchI GAGTC 3 cut(s) 159, 416, 521
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 313
SfcI CTRYAG 2 cut(s) 344, 534
SinI GGWCC 2 cut(s) 6, 300
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 502
SsiI CCGC 1 cut(s) 72
SspMI CTAG 3 cut(s) 740, 840, 856
StyD4I CCNGG 1 cut(s) 321
TaaI ACNGT 4 cut(s) 200, 454, 611, 807
TaiI ACGT 1 cut(s) 569
TasI AATT 1 cut(s) 502
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 3 cut(s) 456, 501, 717
Tru9I TTAA 3 cut(s) 456, 501, 717
TscAI CASTG 2 cut(s) 112, 583
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 5 cut(s) 90, 251, 519, 522, 767
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 4 cut(s) 28, 232, 256, 816
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 112, 583
VpaK11BI GGWCC 2 cut(s) 6, 300
XceI RCATGY 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 3 cut(s) 740, 840, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.