MD11G1123600.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010861
Physical Location & Seq
Forward (+)
11357890 .. 11361637
3748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1123600.v1.1.491

Sequence Viewer

Length: 945 bp
ATGGTGGTTTCTCTTGCTGGGTTCGCGGGTGCGTGCTACAGGAACACCTTCCTCATGTGGGTGTCCCTGTTCGTCATGTTCTTCGTCATCGCCGCCCTCATCGGCTTCATTATCTTCGCCTACGCCGTCACTGACAAGGGCTCCGGGAGGTCGTTGACGAATAAGGGTTACAAGGACTACTACCTGCAGGACTATTCCGGCTGGCTGAAGGACCGCGTGGCGGATAATGGCTACTGGAGGAAGATTGCGTCTTGTGTGAGGGACTCAAAAGCTTGTCAAAAGATTGGGAGGAGTGTGAATGGTGTGCCTGAGACTGCTGACATGTTTTACAGCAGAAGGCTCAACTCTATCCAGCCCCCTACAGAATGTGGATATAGCTATGTGAACGAGACCGTCTGGACCACCGGAGCAGGTTTTTCCGGGACCAATACGGACTGCACACTGTGGAGCAATGACCAACAGCAACTCTGCTATTCCTGCAATTCTTGCAGAGCTGGTCTCTTGGCCAGCATCAGGAAGAGCTGGAGGAAGGTCTCTGTGATCAACATTGTTGTGTTGATCATCCTAGTTATCTTCTATGTGATCGGTTGTGCAGCATTCCGCAACAACCGTCGGATTGACAACGACGAACCCTACGGCGAGGCTCGGATGACAAAATCACACCCAAACTCTATTAACCATCTGCTGGAGAGGAAAAAAGATGCCAATTTGCTTGATTTTTCTTCGGTATGTGTTTACACTAACTGCAATCACGACGTGGAAAGCGGTAGTGTAGGTGCTTCATGTGGTGGCGTTGTGTTGTGTTGGAGTGTGATGTACTTACTCCGACGATGTGAAAAAGTGTCCACACTTTGTACATATACGTCTGAAGGAAAACGTAGTTTGCATTACTCTTTCTATGAAAGTTTCACAGATAACATTGAAATGCAGAAAATCAAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

315

Amino Acids

35.4

Weight (kDa)

8.81

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 1 - 198 2.1e-20 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 192, 401
AccB7I CCANNNNNTGG 1 cut(s) 685
AccII CGCG 2 cut(s) 26, 216
AciI CCGC 6 cut(s) 26, 93, 214, 221, 601, 765
AcoI YGGCCR 1 cut(s) 504
AcuI CTGAAG 2 cut(s) 227, 888
AdeI CACNNNGTG 2 cut(s) 444, 757
AfaI GTAC 2 cut(s) 818, 856
AfiI CCNNNNNNNGG 4 cut(s) 58, 220, 513, 685
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 1 cut(s) 923
AjiI CACGTC 1 cut(s) 757
AluBI AGCT 4 cut(s) 272, 378, 494, 522
AluI AGCT 4 cut(s) 272, 378, 494, 522
Alw26I GTCTC 4 cut(s) 305, 383, 503, 538
AoxI GGCC 1 cut(s) 504
ApeKI GCWGC 1 cut(s) 593
Asp700I GAANNNNTTC 1 cut(s) 47
AspS9I GGNCC 3 cut(s) 211, 399, 423
AsuC2I CCSGG 2 cut(s) 145, 421
AvaII GGWCC 3 cut(s) 211, 399, 423
BalI TGGCCA 1 cut(s) 506
BanII GRGCYC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 605
BccI CCATC 1 cut(s) 687
BceAI ACGGC 2 cut(s) 110, 652
BclI TGATCA 2 cut(s) 540, 558
BcnI CCSGG 2 cut(s) 145, 421
BcoDI GTCTC 4 cut(s) 305, 383, 503, 538
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 3 cut(s) 37, 185, 360
BfuAI ACCTGC 2 cut(s) 192, 401
BisI GCNGC 2 cut(s) 93, 594
BlsI GCNGC 2 cut(s) 94, 595
Bme1390I CCNGG 2 cut(s) 145, 421
Bme18I GGWCC 3 cut(s) 211, 399, 423
BmgBI CACGTC 1 cut(s) 757
BmgT120I GGNCC 3 cut(s) 211, 399, 423
BmiI GGNNCC 2 cut(s) 142, 424
BmrFI CCNGG 2 cut(s) 145, 421
BmsI GCATC 2 cut(s) 519, 691
BplI GAGNNNNNCTC 2 cut(s) 483, 515
BpmI CTGGAG 3 cut(s) 256, 544, 707
BpuMI CCSGG 2 cut(s) 145, 421
BsaI GGTCTC 3 cut(s) 383, 503, 538
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 220, 513, 685
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 457
BseGI GGATG 2 cut(s) 561, 654
BseLI CCNNNNNNNGG 4 cut(s) 58, 220, 513, 685
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 605
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 2 cut(s) 421, 612
Bsh1236I CGCG 2 cut(s) 26, 216
BshFI GGCC 1 cut(s) 506
BsiSI CCGG 4 cut(s) 144, 198, 405, 420
BslFI GGGAC 3 cut(s) 49, 275, 436
BslI CCNNNNNNNGG 4 cut(s) 58, 220, 513, 685
BsmAI GTCTC 4 cut(s) 305, 383, 503, 538
BsmFI GGGAC 3 cut(s) 49, 275, 436
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 1 cut(s) 506
Bso31I GGTCTC 3 cut(s) 383, 503, 538
Bsp1286I GDGCHC 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 854
Bsp143I GATC 3 cut(s) 540, 558, 582
BspACI CCGC 6 cut(s) 26, 93, 214, 221, 601, 765
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 2 cut(s) 26, 216
BspLI GGNNCC 2 cut(s) 142, 424
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 2 cut(s) 192, 401
BspQI GCTCTTC 1 cut(s) 512
BspTNI GGTCTC 3 cut(s) 383, 503, 538
BsrDI GCAATG 1 cut(s) 457
BsrGI TGTACA 1 cut(s) 854
BsrI ACTGG 1 cut(s) 239
BssMI GATC 3 cut(s) 540, 558, 582
Bst4CI ACNGT 3 cut(s) 394, 444, 611
Bst6I CTCTTC 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 486
BstAUI TGTACA 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 34, 203, 508
BstDEI CTNAG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 561, 654
BstFNI CGCG 2 cut(s) 26, 216
BstKTI GATC 3 cut(s) 543, 561, 585
BstMAI GTCTC 4 cut(s) 305, 383, 503, 538
BstMBI GATC 3 cut(s) 540, 558, 582
BstMWI GCNNNNNNNGC 3 cut(s) 23, 477, 486
BstNSI RCATGY 1 cut(s) 325
BstSCI CCNGG 2 cut(s) 143, 419
BstSFI CTRYAG 3 cut(s) 37, 185, 360
BstUI CGCG 2 cut(s) 26, 216
BstV1I GCAGC 1 cut(s) 605
BsuRI GGCC 1 cut(s) 506
BtgZI GCGATG 1 cut(s) 73
BtrI CACGTC 1 cut(s) 757
BtsCI GGATG 2 cut(s) 561, 654
BtsIMutI CAGTG 2 cut(s) 129, 440
BveI ACCTGC 2 cut(s) 192, 401
Cac8I GCNNGC 3 cut(s) 34, 203, 508
Cfr13I GGNCC 3 cut(s) 211, 399, 423
CseI GACGC 1 cut(s) 237
Csp6I GTAC 2 cut(s) 817, 855
CviAII CATG 4 cut(s) 55, 76, 322, 783
CviQI GTAC 2 cut(s) 817, 855
DdeI CTNAG 1 cut(s) 309
DpnI GATC 3 cut(s) 542, 560, 584
DpnII GATC 3 cut(s) 540, 558, 582
DraIII CACNNNGTG 2 cut(s) 444, 757
EaeI YGGCCR 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 3 cut(s) 383, 503, 538
Eco47I GGWCC 3 cut(s) 211, 399, 423
Eco57I CTGAAG 2 cut(s) 227, 888
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 4 cut(s) 58, 79, 325, 786
FaqI GGGAC 3 cut(s) 49, 275, 436
FatI CATG 4 cut(s) 54, 75, 321, 782
FauI CCCGC 1 cut(s) 19
FbaI TGATCA 2 cut(s) 540, 558
Fnu4HI GCNGC 2 cut(s) 93, 594
FokI GGATG 2 cut(s) 548, 661
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 2 cut(s) 93, 594
FspBI CTAG 1 cut(s) 566
GluI GCNGC 2 cut(s) 93, 594
GsaI CCCAGC 1 cut(s) 21
GsuI CTGGAG 3 cut(s) 256, 544, 707
HaeIII GGCC 1 cut(s) 506
HapII CCGG 4 cut(s) 144, 198, 405, 420
HgaI GACGC 1 cut(s) 237
Hin1II CATG 4 cut(s) 58, 79, 325, 786
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 1 cut(s) 263
HpaII CCGG 4 cut(s) 144, 198, 405, 420
Hpy166II GTNNAC 4 cut(s) 156, 385, 736, 846
Hpy188I TCNGA 4 cut(s) 615, 648, 827, 868
Hpy188III TCNNGA 3 cut(s) 397, 514, 752
Hpy8I GTNNAC 4 cut(s) 156, 385, 736, 846
Hpy99I CGWCG 4 cut(s) 615, 629, 758, 831
HpyAV CCTTC 5 cut(s) 58, 202, 330, 523, 863
HpyCH4III ACNGT 3 cut(s) 394, 444, 611
HpyCH4IV ACGT 3 cut(s) 756, 863, 877
HpyCH4V TGCA 8 cut(s) 187, 438, 480, 489, 593, 747, 886, 928
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 477, 486
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 3 cut(s) 756, 863, 877
Hsp92II CATG 4 cut(s) 58, 79, 325, 786
Ksp22I TGATCA 2 cut(s) 540, 558
Kzo9I GATC 3 cut(s) 540, 558, 582
LguI GCTCTTC 1 cut(s) 512
LmnI GCTCC 3 cut(s) 146, 407, 447
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 2 cut(s) 519, 691
MaeI CTAG 1 cut(s) 566
MaeII ACGT 3 cut(s) 756, 863, 877
MaeIII GTNAC 2 cut(s) 127, 167
MalI GATC 3 cut(s) 542, 560, 584
MboI GATC 3 cut(s) 540, 558, 582
MboII GAAGA 6 cut(s) 73, 106, 253, 529, 565, 714
MhlI GDGCHC 1 cut(s) 143
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 2 cut(s) 481, 706
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 3 cut(s) 593, 785, 850
MnlI CCTC 9 cut(s) 62, 107, 141, 231, 252, 282, 519, 634, 684
Mox20I TGGCCA 1 cut(s) 506
MroXI GAANNNNTTC 1 cut(s) 47
MscI TGGCCA 1 cut(s) 506
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 3 cut(s) 59, 551, 923
Msp20I TGGCCA 1 cut(s) 506
MspI CCGG 4 cut(s) 144, 198, 405, 420
MspR9I CCNGG 2 cut(s) 145, 421
Mva1269I GAATGC 1 cut(s) 596
MvnI CGCG 2 cut(s) 26, 216
MwoI GCNNNNNNNGC 3 cut(s) 23, 477, 486
NciI CCSGG 2 cut(s) 145, 421
NdeII GATC 3 cut(s) 540, 558, 582
NlaIII CATG 4 cut(s) 58, 79, 325, 786
NlaIV GGNNCC 2 cut(s) 142, 424
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 325
PciI ACATGT 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 512
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 596
PdmI GAANNNNTTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 685
PfoI TCCNGGA 2 cut(s) 143, 419
PkrI GCNGC 2 cut(s) 94, 595
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PscI ACATGT 1 cut(s) 321
PspFI CCCAGC 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 142, 424
PspPI GGNCC 3 cut(s) 211, 399, 423
PstI CTGCAG 1 cut(s) 189
RsaI GTAC 2 cut(s) 818, 856
RsaNI GTAC 2 cut(s) 817, 855
RseI CAYNNNNRTG 3 cut(s) 59, 551, 923
SapI GCTCTTC 1 cut(s) 512
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 2 cut(s) 93, 594
Sau3AI GATC 3 cut(s) 540, 558, 582
Sau96I GGNCC 3 cut(s) 211, 399, 423
SbfI CCTGCAGG 1 cut(s) 189
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 145, 421
SdaI CCTGCAGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 2 cut(s) 519, 691
SfcI CTRYAG 3 cut(s) 37, 185, 360
SinI GGWCC 3 cut(s) 211, 399, 423
SmiMI CAYNNNNRTG 3 cut(s) 59, 551, 923
Sse8387I CCTGCAGG 1 cut(s) 189
Sse9I AATT 2 cut(s) 481, 706
SsiI CCGC 6 cut(s) 26, 93, 214, 221, 601, 765
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 2 cut(s) 143, 419
TaaI ACNGT 3 cut(s) 394, 444, 611
TaiI ACGT 3 cut(s) 759, 866, 880
TasI AATT 2 cut(s) 481, 706
TatI WGTACW 2 cut(s) 816, 854
TauI GCSGC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TscAI CASTG 2 cut(s) 136, 447
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 97, 771, 915
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 2 cut(s) 136, 447
Van91I CCANNNNNTGG 1 cut(s) 685
VpaK11BI GGWCC 3 cut(s) 211, 399, 423
XceI RCATGY 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.