MD11G1124400.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010861
Physical Location & Seq
Reverse (-)
11416344 .. 11420954
4611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1124400.v1.1.491

Sequence Viewer

Length: 861 bp
ATGATGAGGACCAGCAACCACTTGATTGGACTACTAAATTTCGTAACTTTCCTCCTCTCGATCCCCATCCTGGGCGGAGGAATCTGGCTGAGCAGCCGCGCCAACAACACCGACTGCCTCAAGTTCCTCCAATGGCCGCTCATCGTCATAGGGGCCGCAATCATGGTGGTTTCTCTTGCTGGGTTCGCTGGTGCGTGCTACAGGAACACCTTCCTCATGTGGGTGTACCTGTTCGTCATGTTCTTCGTCATCGCCGCCCTCATCGGCTTCATTATCTTCGCCTACGCCGTCACTGACAAGGGCTCCGGGAGGTCGTTGACGAATAAGGGTTACAAGGACTACTACCTGCAGGACTATTCCGGCTGGCTGAAGGACCGCGTGGCGGATAATGGCTACTGGAGGAAGATTGCGTCTTGTGTGAGGGACTCAAAAGCTTGTCAAAAGATTGGGAGGAGTGTGAATGGTGTGCCTGAGACTGCTGACATGTTTTTCAGCAGAAAGCTCAACTCTATCCAGTCCGGCTGCTGCAAGCCCCCTACAGAATGTGGATATAGCTATGTGAACGAGACCGTCTGGACCACCGGAGCAGGTTTTTCCGGGACCAGTACGGACTGCACACTGTGGAGCAATGACCAACAGCAACTCTGCTATTCCTGCAATTCTTGCAGAGCTGGTCTCTTGGCCAGCATCAGGAAGAGCTGGAGGAAGGTCTCTGTGGTCAACATTGTTGTGTTGATCATCCTAGTTATCTTCTATGTGATCGGTTGTGCAGCATTCCGCAACAACCGTCGGATTGACAACGACGAACCCTACGGCGAGGCTCGGATGACAAAATCACACCCAAGTGCTTTCCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

287

Amino Acids

31.92

Weight (kDa)

9.2

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 257 3.8e-39 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 354, 578
AccBSI CCGCTC 1 cut(s) 139
AccII CGCG 2 cut(s) 99, 378
AciI CCGC 8 cut(s) 75, 97, 137, 156, 255, 376, 383, 778
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 2 cut(s) 134, 681
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 389
AdeI CACNNNGTG 1 cut(s) 621
AfaI GTAC 2 cut(s) 227, 607
AfiI CCNNNNNNNGG 5 cut(s) 70, 71, 220, 382, 690
AflIII ACRYGT 1 cut(s) 483
AjnI CCWGG 1 cut(s) 69
AleI CACNNNNGTG 1 cut(s) 843
AluBI AGCT 5 cut(s) 434, 502, 555, 671, 699
AluI AGCT 5 cut(s) 434, 502, 555, 671, 699
Alw26I GTCTC 4 cut(s) 467, 560, 680, 715
AlwI GGATC 1 cut(s) 55
AoxI GGCC 3 cut(s) 134, 153, 681
ApeKI GCWGC 4 cut(s) 93, 522, 525, 770
ApoI RAATTY 1 cut(s) 37
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 5 cut(s) 9, 153, 373, 576, 600
AsuC2I CCSGG 2 cut(s) 307, 598
AvaII GGWCC 4 cut(s) 9, 373, 576, 600
BalI TGGCCA 1 cut(s) 683
BanII GRGCYC 1 cut(s) 305
BbvI GCAGC 4 cut(s) 105, 509, 512, 782
BccI CCATC 1 cut(s) 74
BceAI ACGGC 2 cut(s) 272, 829
BciT130I CCWGG 1 cut(s) 71
BclI TGATCA 1 cut(s) 735
BcnI CCSGG 2 cut(s) 307, 598
BcoDI GTCTC 4 cut(s) 467, 560, 680, 715
BfaI CTAG 1 cut(s) 743
BfmI CTRYAG 3 cut(s) 199, 347, 537
BfuAI ACCTGC 2 cut(s) 354, 578
BisI GCNGC 8 cut(s) 94, 97, 137, 156, 255, 523, 526, 771
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 8 cut(s) 95, 98, 138, 157, 256, 524, 527, 772
Bme1390I CCNGG 3 cut(s) 71, 307, 598
Bme18I GGWCC 4 cut(s) 9, 373, 576, 600
BmgT120I GGNCC 5 cut(s) 9, 153, 373, 576, 600
BmiI GGNNCC 3 cut(s) 154, 304, 601
BmrFI CCNGG 3 cut(s) 71, 307, 598
BmsI GCATC 1 cut(s) 696
BplI GAGNNNNNCTC 2 cut(s) 660, 692
BpmI CTGGAG 2 cut(s) 418, 721
Bpu1102I GCTNAGC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 2 cut(s) 307, 598
BsaBI GATNNNNATC 1 cut(s) 65
BsaI GGTCTC 3 cut(s) 560, 680, 715
BsaJI CCNNGG 1 cut(s) 70
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 71, 220, 382, 690
Bse1I ACTGG 3 cut(s) 401, 514, 603
Bse3DI GCAATG 1 cut(s) 634
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 3 cut(s) 66, 738, 831
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 5 cut(s) 70, 71, 220, 382, 690
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 2 cut(s) 80, 462
BseNI ACTGG 3 cut(s) 401, 514, 603
BseRI GAGGAG 2 cut(s) 44, 466
BseXI GCAGC 4 cut(s) 105, 509, 512, 782
BseYI CCCAGC 1 cut(s) 179
BsgI GTGCAG 2 cut(s) 598, 789
Bsh1236I CGCG 2 cut(s) 99, 378
BshFI GGCC 3 cut(s) 136, 155, 683
BsiSI CCGG 5 cut(s) 306, 360, 519, 582, 597
BslFI GGGAC 2 cut(s) 437, 613
BslI CCNNNNNNNGG 5 cut(s) 70, 71, 220, 382, 690
BsmAI GTCTC 4 cut(s) 467, 560, 680, 715
BsmFI GGGAC 2 cut(s) 437, 613
BsmI GAATGC 1 cut(s) 773
BsnI GGCC 3 cut(s) 136, 155, 683
Bso31I GGTCTC 3 cut(s) 560, 680, 715
Bsp1286I GDGCHC 1 cut(s) 305
Bsp143I GATC 3 cut(s) 60, 735, 759
Bsp1720I GCTNAGC 1 cut(s) 89
BspACI CCGC 8 cut(s) 75, 97, 137, 156, 255, 376, 383, 778
BspANI GGCC 3 cut(s) 136, 155, 683
BspCNI CTCAG 2 cut(s) 81, 463
BspFNI CGCG 2 cut(s) 99, 378
BspLI GGNNCC 3 cut(s) 154, 304, 601
BspMAI CTGCAG 1 cut(s) 351
BspMI ACCTGC 2 cut(s) 354, 578
BspPI GGATC 1 cut(s) 55
BspQI GCTCTTC 1 cut(s) 689
BspTNI GGTCTC 3 cut(s) 560, 680, 715
BsrBI CCGCTC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 634
BsrI ACTGG 3 cut(s) 401, 514, 603
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 3 cut(s) 60, 735, 759
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 3 cut(s) 571, 621, 788
Bst6I CTCTTC 1 cut(s) 689
BstAPI GCANNNNNTGC 1 cut(s) 663
BstC8I GCNNGC 4 cut(s) 196, 365, 530, 685
BstDEI CTNAG 2 cut(s) 89, 471
BstF5I GGATG 3 cut(s) 66, 738, 831
BstFNI CGCG 2 cut(s) 99, 378
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 3 cut(s) 63, 738, 762
BstMAI GTCTC 4 cut(s) 467, 560, 680, 715
BstMBI GATC 3 cut(s) 60, 735, 759
BstMWI GCNNNNNNNGC 3 cut(s) 185, 654, 663
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 487
BstSCI CCNGG 3 cut(s) 69, 305, 596
BstSFI CTRYAG 3 cut(s) 199, 347, 537
BstUI CGCG 2 cut(s) 99, 378
BstV1I GCAGC 4 cut(s) 105, 509, 512, 782
BstXI CCANNNNNNTGG 1 cut(s) 26
BsuRI GGCC 3 cut(s) 136, 155, 683
BtgZI GCGATG 1 cut(s) 235
BtsCI GGATG 3 cut(s) 66, 738, 831
BtsIMutI CAGTG 2 cut(s) 291, 617
BveI ACCTGC 2 cut(s) 354, 578
Cac8I GCNNGC 4 cut(s) 196, 365, 530, 685
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 5 cut(s) 9, 153, 373, 576, 600
CseI GACGC 1 cut(s) 399
Csp6I GTAC 2 cut(s) 226, 606
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 4 cut(s) 163, 217, 238, 484
CviQI GTAC 2 cut(s) 226, 606
DdeI CTNAG 2 cut(s) 89, 471
DpnI GATC 3 cut(s) 62, 737, 761
DpnII GATC 3 cut(s) 60, 735, 759
DraIII CACNNNGTG 1 cut(s) 621
EaeI YGGCCR 2 cut(s) 134, 681
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
EciI GGCGGA 2 cut(s) 90, 398
Eco24I GRGCYC 1 cut(s) 305
Eco31I GGTCTC 3 cut(s) 560, 680, 715
Eco47I GGWCC 4 cut(s) 9, 373, 576, 600
Eco57I CTGAAG 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 305
FaeI CATG 4 cut(s) 166, 220, 241, 487
FaiI YATR 8 cut(s) 149, 164, 218, 239, 485, 552, 558, 756
FaqI GGGAC 2 cut(s) 437, 613
FatI CATG 4 cut(s) 162, 216, 237, 483
FbaI TGATCA 1 cut(s) 735
Fnu4HI GCNGC 8 cut(s) 94, 97, 137, 156, 255, 523, 526, 771
FokI GGATG 3 cut(s) 53, 725, 838
FriOI GRGCYC 1 cut(s) 305
Fsp4HI GCNGC 8 cut(s) 94, 97, 137, 156, 255, 523, 526, 771
FspBI CTAG 1 cut(s) 743
GlaI GCGC 1 cut(s) 100
GluI GCNGC 8 cut(s) 94, 97, 137, 156, 255, 523, 526, 771
GsaI CCCAGC 1 cut(s) 183
GsuI CTGGAG 2 cut(s) 418, 721
HaeIII GGCC 3 cut(s) 136, 155, 683
HapII CCGG 5 cut(s) 306, 360, 519, 582, 597
HgaI GACGC 1 cut(s) 399
HhaI GCGC 1 cut(s) 101
Hin1II CATG 4 cut(s) 166, 220, 241, 487
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 2 cut(s) 318, 721
HindII GTYRAC 2 cut(s) 318, 721
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 2 cut(s) 81, 425
HpaII CCGG 5 cut(s) 306, 360, 519, 582, 597
Hpy166II GTNNAC 4 cut(s) 226, 318, 562, 721
Hpy188I TCNGA 2 cut(s) 792, 825
Hpy188III TCNNGA 3 cut(s) 58, 574, 691
Hpy8I GTNNAC 4 cut(s) 226, 318, 562, 721
Hpy99I CGWCG 2 cut(s) 792, 806
HpyAV CCTTC 3 cut(s) 220, 364, 700
HpyCH4III ACNGT 3 cut(s) 571, 621, 788
HpyCH4V TGCA 6 cut(s) 349, 528, 615, 657, 666, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 654, 663
HpyF3I CTNAG 2 cut(s) 89, 471
Hsp92II CATG 4 cut(s) 166, 220, 241, 487
HspAI GCGC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 735
Kzo9I GATC 3 cut(s) 60, 735, 759
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 308, 584, 624
Lsp1109I GCAGC 4 cut(s) 105, 509, 512, 782
LweI GCATC 1 cut(s) 696
MaeI CTAG 1 cut(s) 743
MaeIII GTNAC 3 cut(s) 43, 289, 329
MalI GATC 3 cut(s) 62, 737, 761
MbiI CCGCTC 1 cut(s) 139
MboI GATC 3 cut(s) 60, 735, 759
MboII GAAGA 5 cut(s) 235, 268, 415, 706, 742
MhlI GDGCHC 1 cut(s) 305
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 2 cut(s) 37, 658
MluNI TGGCCA 1 cut(s) 683
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 770
Mox20I TGGCCA 1 cut(s) 683
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 683
MslI CAYNNNNRTG 3 cut(s) 221, 728, 843
Msp20I TGGCCA 1 cut(s) 683
MspI CCGG 5 cut(s) 306, 360, 519, 582, 597
MspR9I CCNGG 3 cut(s) 71, 307, 598
Mva1269I GAATGC 1 cut(s) 773
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 2 cut(s) 99, 378
MwoI GCNNNNNNNGC 3 cut(s) 185, 654, 663
NciI CCSGG 2 cut(s) 307, 598
NdeII GATC 3 cut(s) 60, 735, 759
NlaIII CATG 4 cut(s) 166, 220, 241, 487
NlaIV GGNNCC 3 cut(s) 154, 304, 601
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 1 cut(s) 487
OliI CACNNNNGTG 1 cut(s) 843
PciI ACATGT 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 689
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 773
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 81
PfoI TCCNGGA 2 cut(s) 305, 596
PkrI GCNGC 8 cut(s) 95, 98, 138, 157, 256, 524, 527, 772
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PscI ACATGT 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 69
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 3 cut(s) 154, 304, 601
PspPI GGNCC 5 cut(s) 9, 153, 373, 576, 600
PstI CTGCAG 1 cut(s) 351
RsaI GTAC 2 cut(s) 227, 607
RsaNI GTAC 2 cut(s) 226, 606
RseI CAYNNNNRTG 3 cut(s) 221, 728, 843
SapI GCTCTTC 1 cut(s) 689
SatI GCNGC 8 cut(s) 94, 97, 137, 156, 255, 523, 526, 771
Sau3AI GATC 3 cut(s) 60, 735, 759
Sau96I GGNCC 5 cut(s) 9, 153, 373, 576, 600
SbfI CCTGCAGG 1 cut(s) 351
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 3 cut(s) 71, 307, 598
SdaI CCTGCAGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 305
SfaNI GCATC 1 cut(s) 696
SfcI CTRYAG 3 cut(s) 199, 347, 537
SinI GGWCC 4 cut(s) 9, 373, 576, 600
SmiMI CAYNNNNRTG 3 cut(s) 221, 728, 843
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse8387I CCTGCAGG 1 cut(s) 351
Sse9I AATT 2 cut(s) 37, 658
SsiI CCGC 8 cut(s) 75, 97, 137, 156, 255, 376, 383, 778
SspMI CTAG 1 cut(s) 743
StyD4I CCNGG 3 cut(s) 69, 305, 596
TaaI ACNGT 3 cut(s) 571, 621, 788
TaqI TCGA 1 cut(s) 59
TasI AATT 2 cut(s) 37, 658
TauI GCSGC 4 cut(s) 99, 139, 158, 257
TfiI GAWTC 1 cut(s) 81
TscAI CASTG 2 cut(s) 298, 624
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 4 cut(s) 93, 522, 525, 770
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 298, 624
VpaK11BI GGWCC 4 cut(s) 9, 373, 576, 600
XapI RAATTY 1 cut(s) 37
XceI RCATGY 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.