Rroxscaffold_1G00019600

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010861
Physical Location & Seq
Forward (+)
23955458 .. 23958333
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019600.1

Sequence Viewer

Length: 903 bp
ATGAGGTCCAGCAACCACTTGATAGGTGTCCTCAACTTCACCACCTTCCTCCTCTCAATCCCTATCCTCGGCGGTGGAATCTGGCTGAGCAGCCGAGCCAACAACACTGACTGCCTCAAGTTCCTCCAATGGCCTCTCATAGTCATCGGAGCCTCCATCATGGTCGTCTCCTTAGCCGGCTTCGCCGGAGCTTGTTACCGCAACACTTTCCTCATGTGGGTCTACCTCTTCGTCATGTTCTTCATCATAGCTGCTCTGATCGGCTTCATCATTTTCGCTTACGCCGTGACGGATAAAGGGTCCGGCAGGGGGCTCGTAAACCAGGCTTATAAGGACTACTATCTGGATGACTACTCTGGCTGGCTAAGGGACCGCGTAGCGGATAATAGCTACTGGAGGAAGATTGCTTCTTGTGTGAGGGACTCTAAGGTCTGCAAGAAGATGGACAGGACTGTTAATGGTGTGCCGGAGACTGCTGAGATGTTCTACAACAGAAAGCTTAATTCTGTTGAGTTGTTCAGTAGATCAGGATCCTCAAACGAAAAGAATGTTCTTCTAGTCTGGCTGCTGCAAGCCCCTACAGAGTGTGGCTACACCTATGTGAATGAGACATTTTGGACCACTGGAGGAGGCTTGGTGGGAACAAATCCCGACTGTTCTATGTGGAACAATGATCAGCAGGAACTCTGCTATTCCTGTAACTCTTGCAGGGCTGGTGTGCTGGCCAGTATTAGGAAGAGTTGGAGAAAGGTCTCTGTCATTAACATTGTTGTATTGATCATCCTAGTGATCTTCTATGTGATTGGCTGTGCAGCGTTCAGAAACAATCGTCGGATTGACAATGATGAACCGTATGGTGAGGCTCGGATGACAAAGGCGCAACCTAGCCCTTTCCAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

300

Amino Acids

33.83

Weight (kDa)

8.97

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 271 5.2e-34 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AasI GACNNNNNNGTC 1 cut(s) 428
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 375
AciI CCGC 4 cut(s) 72, 199, 373, 380
AclWI GGATC 2 cut(s) 525, 538
AcoI YGGCCR 1 cut(s) 723
AfiI CCNNNNNNNGG 5 cut(s) 68, 217, 309, 379, 732
AjnI CCWGG 1 cut(s) 321
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 4 cut(s) 191, 251, 390, 499
AluI AGCT 4 cut(s) 191, 251, 390, 499
Alw26I GTCTC 4 cut(s) 172, 464, 602, 757
AlwI GGATC 2 cut(s) 525, 538
AoxI GGCC 2 cut(s) 131, 723
ApeKI GCWGC 5 cut(s) 90, 251, 565, 568, 812
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 4 cut(s) 6, 300, 370, 618
AsuHPI GGTGA 2 cut(s) 31, 869
AvaII GGWCC 4 cut(s) 6, 300, 370, 618
BalI TGGCCA 1 cut(s) 725
BamHI GGATCC 1 cut(s) 530
BanII GRGCYC 1 cut(s) 315
BbvI GCAGC 5 cut(s) 102, 238, 552, 555, 824
BccI CCATC 2 cut(s) 164, 436
BceAI ACGGC 1 cut(s) 269
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 1 cut(s) 323
BclI TGATCA 2 cut(s) 673, 777
BcoDI GTCTC 4 cut(s) 172, 464, 602, 757
BfaI CTAG 4 cut(s) 557, 785, 885, 901
BfmI CTRYAG 1 cut(s) 579
BisI GCNGC 5 cut(s) 91, 252, 566, 569, 813
BlpI GCTNAGC 1 cut(s) 86
BlsI GCNGC 5 cut(s) 92, 253, 567, 570, 814
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 4 cut(s) 6, 300, 370, 618
BmgT120I GGNCC 4 cut(s) 6, 300, 370, 618
BmiI GGNNCC 4 cut(s) 151, 301, 371, 532
BmrFI CCNGG 1 cut(s) 323
BpmI CTGGAG 2 cut(s) 415, 645
Bpu10I CCTNAGC 2 cut(s) 172, 365
Bpu1102I GCTNAGC 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 757
BsaJI CCNNGG 1 cut(s) 67
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 217, 309, 379, 732
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 3 cut(s) 398, 628, 726
Bse8I GATNNNNATC 1 cut(s) 529
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 3 cut(s) 352, 780, 873
BseJI GATNNNNATC 1 cut(s) 529
BseLI CCNNNNNNNGG 5 cut(s) 68, 217, 309, 379, 732
BseMII CTCAG 2 cut(s) 77, 468
BseNI ACTGG 3 cut(s) 398, 628, 726
BseRI GAGGAG 2 cut(s) 41, 642
BseXI GCAGC 5 cut(s) 102, 238, 552, 555, 824
BsgI GTGCAG 1 cut(s) 831
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 2 cut(s) 133, 725
BsiSI CCGG 4 cut(s) 177, 186, 303, 467
BslFI GGGAC 2 cut(s) 383, 434
BslI CCNNNNNNNGG 5 cut(s) 68, 217, 309, 379, 732
BsmAI GTCTC 4 cut(s) 172, 464, 602, 757
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 2 cut(s) 383, 434
BsnI GGCC 2 cut(s) 133, 725
Bso31I GGTCTC 1 cut(s) 757
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 6 cut(s) 258, 524, 530, 673, 777, 789
Bsp1720I GCTNAGC 1 cut(s) 86
BspACI CCGC 4 cut(s) 72, 199, 373, 380
BspANI GGCC 2 cut(s) 133, 725
BspCNI CTCAG 2 cut(s) 78, 469
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 4 cut(s) 151, 301, 371, 532
BspPI GGATC 2 cut(s) 525, 538
BspTNI GGTCTC 1 cut(s) 757
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 3 cut(s) 398, 628, 726
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 6 cut(s) 258, 524, 530, 673, 777, 789
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 3 cut(s) 454, 656, 852
Bst6I CTCTTC 2 cut(s) 233, 731
BstC8I GCNNGC 4 cut(s) 178, 362, 573, 723
BstDEI CTNAG 5 cut(s) 86, 172, 365, 426, 477
BstF5I GGATG 3 cut(s) 352, 780, 873
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 6 cut(s) 261, 527, 533, 676, 780, 792
BstMAI GTCTC 4 cut(s) 172, 464, 602, 757
BstMBI GATC 6 cut(s) 258, 524, 530, 673, 777, 789
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 5 cut(s) 102, 238, 552, 555, 824
BstX2I RGATCY 1 cut(s) 530
BstYI RGATCY 1 cut(s) 530
BsuRI GGCC 2 cut(s) 133, 725
BtsCI GGATG 3 cut(s) 352, 780, 873
BtsIMutI CAGTG 2 cut(s) 105, 621
Cac8I GCNNGC 4 cut(s) 178, 362, 573, 723
CfoI GCGC 1 cut(s) 880
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 4 cut(s) 6, 300, 370, 618
CviAII CATG 3 cut(s) 160, 214, 235
DdeI CTNAG 5 cut(s) 86, 172, 365, 426, 477
DpnI GATC 6 cut(s) 260, 526, 532, 675, 779, 791
DpnII GATC 6 cut(s) 258, 524, 530, 673, 777, 789
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 2 cut(s) 233, 731
EarI CTCTTC 2 cut(s) 233, 731
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 757
Eco47I GGWCC 4 cut(s) 6, 300, 370, 618
EcoRII CCWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 3 cut(s) 163, 217, 238
FaqI GGGAC 2 cut(s) 383, 434
FatI CATG 3 cut(s) 159, 213, 234
FbaI TGATCA 2 cut(s) 673, 777
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 5 cut(s) 91, 252, 566, 569, 813
FokI GGATG 3 cut(s) 359, 767, 880
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 5 cut(s) 91, 252, 566, 569, 813
FspBI CTAG 4 cut(s) 557, 785, 885, 901
GlaI GCGC 1 cut(s) 879
GluI GCNGC 5 cut(s) 91, 252, 566, 569, 813
GsuI CTGGAG 2 cut(s) 415, 645
HaeIII GGCC 2 cut(s) 133, 725
HapII CCGG 4 cut(s) 177, 186, 303, 467
HhaI GCGC 1 cut(s) 880
Hin1II CATG 3 cut(s) 163, 217, 238
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 2 cut(s) 78, 422
HpaII CCGG 4 cut(s) 177, 186, 303, 467
HphI GGTGA 2 cut(s) 31, 869
Hpy166II GTNNAC 2 cut(s) 223, 319
Hpy188I TCNGA 5 cut(s) 149, 258, 821, 834, 867
Hpy188III TCNNGA 3 cut(s) 344, 528, 650
Hpy8I GTNNAC 2 cut(s) 223, 319
Hpy99I CGWCG 1 cut(s) 834
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 3 cut(s) 454, 656, 852
HpyCH4V TGCA 4 cut(s) 435, 571, 708, 812
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 5 cut(s) 86, 172, 365, 426, 477
Hsp92II CATG 3 cut(s) 163, 217, 238
HspAI GCGC 1 cut(s) 878
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Ksp22I TGATCA 2 cut(s) 673, 777
Kzo9I GATC 6 cut(s) 258, 524, 530, 673, 777, 789
LmnI GCTCC 2 cut(s) 149, 188
Lsp1109I GCAGC 5 cut(s) 102, 238, 552, 555, 824
MaeI CTAG 4 cut(s) 557, 785, 885, 901
MaeIII GTNAC 3 cut(s) 194, 286, 698
MalI GATC 6 cut(s) 260, 526, 532, 675, 779, 791
MboI GATC 6 cut(s) 258, 524, 530, 673, 777, 789
MboII GAAGA 7 cut(s) 220, 232, 412, 451, 545, 748, 784
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 1 cut(s) 502
MluNI TGGCCA 1 cut(s) 725
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 722, 812
Mox20I TGGCCA 1 cut(s) 725
MroNI GCCGGC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 725
MseI TTAA 3 cut(s) 456, 501, 762
MslI CAYNNNNRTG 2 cut(s) 599, 785
Msp20I TGGCCA 1 cut(s) 725
MspI CCGG 4 cut(s) 177, 186, 303, 467
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 1 cut(s) 182
NaeI GCCGGC 1 cut(s) 178
NdeII GATC 6 cut(s) 258, 524, 530, 673, 777, 789
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 3 cut(s) 163, 217, 238
NlaIV GGNNCC 4 cut(s) 151, 301, 371, 532
NmeAIII GCCGAG 2 cut(s) 48, 119
NmuCI GTSAC 1 cut(s) 286
OliI CACNNNNGTG 1 cut(s) 599
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 78
PkrI GCNGC 5 cut(s) 92, 253, 567, 570, 814
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PsiI TTATAA 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 4 cut(s) 151, 301, 371, 532
PspPI GGNCC 4 cut(s) 6, 300, 370, 618
PsuI RGATCY 1 cut(s) 530
RseI CAYNNNNRTG 2 cut(s) 599, 785
SaqAI TTAA 3 cut(s) 456, 501, 762
SatI GCNGC 5 cut(s) 91, 252, 566, 569, 813
Sau3AI GATC 6 cut(s) 258, 524, 530, 673, 777, 789
Sau96I GGNCC 4 cut(s) 6, 300, 370, 618
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 315
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 4 cut(s) 6, 300, 370, 618
SmiMI CAYNNNNRTG 2 cut(s) 599, 785
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 502
SsiI CCGC 4 cut(s) 72, 199, 373, 380
SspMI CTAG 4 cut(s) 557, 785, 885, 901
StyD4I CCNGG 1 cut(s) 321
TaaI ACNGT 3 cut(s) 454, 656, 852
TasI AATT 1 cut(s) 502
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 3 cut(s) 456, 501, 762
Tru9I TTAA 3 cut(s) 456, 501, 762
TscAI CASTG 2 cut(s) 112, 628
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 5 cut(s) 90, 251, 565, 568, 812
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 3 cut(s) 232, 256, 861
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 112, 628
VpaK11BI GGWCC 4 cut(s) 6, 300, 370, 618
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 4 cut(s) 557, 785, 885, 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.