Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006152

Physical Location & Seq

Genomic Coordinates Forward (+)

10311158 .. 10316189

Gene Structure 5032 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1116000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGGAAATTGATCTGAACCATGCAGTGAGTGAGGTGGAGAAGAATGCTTATTGCAATGGGGATTGTGGTGGTGTTTGTGTTTTTTGCTTGTCCTCTTCAACTTCTTCATCTTCTTCCAATTCATCCTCAGCTCCTGTGGCTTCCTCAATTTATCTGGAGCTTTGGCATGCATGTGCTGGCCCTCTCATCTCACTCCCCAAGAAAGGGAATGTGGTTGTCTACTTCCCACAAGGCCACTTGGAGCAAGTTGCCTCCTCCTCCCCTTTCTCTCCCATGGAGATGCCCACCTTTGATCTCCACCCTCAGATCTTCTGCAAGGTTGTCAATGTCCAGCTGCTTGCTAACAAGGAGAATGATGAGGTCTACACACATGTCACTTTGCTTCCTCAGCCTGAGTTTGCAGGAACAAATTTGGAAGGGAAAGAGCTTGAAGAGTTGGGAGTGGATGAGGGGGATGGAGGATCACCTACAAAATCAACCCCTCACATGTTCTGCAAAACGCTTACAGCTTCCGATACCAGTACCCACGGAGGGTTCTCTGTTCCTCGCAGAGCTGCTGAAGACTGTTTCCCTCCTTTGGACTATAAGCAGCAGAGGCCCTCTCAAGAGCTTGTTGCAAAGGACCTTCATGGGGTGGAGTGGAGGTTTCGGCATATTTATAGAGGCCAGCCAAGGCGACATTTGCTCACTACTGGATGGAGTATATTCATAAGCCAAAAGAATCTTGTATCGGGTGATGCTGTGCTATTTTTGAGGGGTGAAAACGGAGAGCTAAGGTTGGGAATTAGAAAAGCTGTTCGACCAAGAAATTGTCTTCCTGATTCAGTTGTAGGAAACCAGAATACTTATCCCAGTGTTCTTTCTCTCGTGGCTAATGCAATATCCACCAAGAGCATGTTTCATGTATTCTACAGTCCAAGAGCAAGCCGTTCAGAGTTTGTCATTCCCTACCAAAAATATGTCAGAAGCATCGCTAATCCAGTGACCTCTGGGACAAGATTCAAAATGAGATTCGATAGGGATGATTCACCTGAAAAAAGGTGTAGTGGTGTAGTGACAGGTATCGCTGACTTGGATCCGTTTCGATGGCCAAACTCAAAATGGAGATGCTTGATGGTTAGATGGGATGAAGATATAGGAAATGATCATCAAGATCGAGTCTCGCCATGGGAGGTTGATCCTTCTGCTTCTCTCCCACCTATGAACATTCAGTCTTCTCCAAGACTGAAGAAACTGCGGACAAGTCTGCAGGGAACCCAACCCATCCACTCCATCACTGCTGGAGGGGCTGGGTTTAAGGACTTTGAGGAATCAGTTAAATCCTCTAAGGTCTTGCAAGGTCAAGAAAATATAGGTTTCATATCACCCCTCTATGGGTGTGATACTGTAAACCGCTCGCAAGATTTTGAGATGCATGCCCCGGCACATCTAAGTCTTGCATCAAATGCAACACAGAAGGCTACTATTGATGAGCTTATGAGGGCTCGCCACACCTCTTACACAGGATTTGCGGAGTCTGATAGATTTCCAAAAGTCTTGCAAGGTCAAGAAATATGCCCGTTGAGATCGTTGACAGGAAAAGCAAATTTCACCCTAGGTGATTGGGAATCCAACATTGGTTGCACACCTTTCAACGTTTATCAACCGCCCAAACCAAATCATTTCTCTCTAGCTTCTGAATCCCTTCCAAATATGTACTTTCCTTATGGTGACGTTCACAGAGCCGGCCAAGAACCCACGATGTGCTCTAATGCTACTAACTTACCCAGAGAGAATATGCAAATCAACCCATATACTGTGCAGATGGGAGTTACAAGAAATGAAGTTGGACGGCCAAATAAACCTAGTGAGCATAAGACACAGGAGAGTACTTCTGCGCTTCCCGCTTTAGTACCAAATCCAAGAAATCCAAATGATGAAGACTATAACGGGACTGTAACTGGGTGTAAACTTTTTGGTTTTTCCTTGACTGGGGAAAATCCCACTCCAAACTCTCAGAGTTCCAGTAAGCGGAGTTGTACAAAGGTTCACAAGCAAGGCAGCCTAGTGGGAAGAGCCATTGATCTCGCGAAACTGAATGGCTATGGGGACCTGCTGACTGAACTGGAGCGGCTATTTGGCATGGAAGGCCTTCTTCGAGATTCTGATGAAGGATGGCGGATCTTGTACACTGATAGCGAGAACGATGTGATGGTTGTTGGGGATGACCCATGGCACGAGTTTTGCAATGTGGTCTCCAAAATCCATATATATACCCAGGAAGAAGTGGAGAAGATGACCATCGGAATGATCAGCGATGATACTCAAAGCAGTTTGGAACAAGCGCCGGTCATGCTTGAAGTGTCAAAGTCCTCATCCGTGAGCCAGCCGGATTCTTCCCCAACAGTAATCCGGGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009889 GO:0009987 GO:0010050 GO:0010158 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

800

Amino Acids

88.45

Weight (kDa)

5.91

Isoelectric Point (pI)

52.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	164 - 265	6.8e-22	B3 DNA binding domain
BRWD_AD	PF25313	295 - 395	6.7e-06	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	313 - 395	9.3e-34	Auxin response factor ancillary domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60450 AT5G60450
fragaria_vesca	FvH4_3g32860 FvH4_3g32860 FvH4_3g32860 FvH4_3g32860 FvH4_3g32860
malus_domestica	MD03G1116000.v1.1 MD11G1132400.v1.1
prunus_persica	Prupe.6G097700_v2.0.a1 Prupe.6G097700_v2.0.a1 Prupe.6G097700_v2.0.a1 Prupe.6G097700_v2.0.a1 Prupe.6G097700_v2.0.a1
pyrus_communis	pycom11g10920
rosa_chinensis	RchiOBHm_Chr5g0060011
rosa_laevigata	RLG00000035338
rosa_multiflora	Rmu_sc0000493.1_g000006
rosa_roxburghii	Rroxscaffold_1G00020650
rosa_rugosa	Rorug05G0327400 Rorug05G0327500 Rorug05G0327600
rosa_samantha	Rh5AG391400 Rh5BG404000 Rh5CG427800 Rh5DG419600
rosa_wichuraiana	Rw5G036880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2100
AccBSI	CCGCTC	2 cut(s)	1395, 2110
AccI	GTMKAC	2 cut(s)	219, 363
AccII	CGCG	1 cut(s)	2069
AciI	CCGC	8 cut(s)	1237, 1393, 1511, 1646, 1884, 2011, 2110, 2158
AclI	AACGTT	1 cut(s)	1635
AclWI	GGATC	5 cut(s)	469, 1070, 1083, 1172, 2168
AcoI	YGGCCR	3 cut(s)	1088, 1726, 1832
AcsI	RAATTY	2 cut(s)	409, 1585
AcuI	CTGAAG	2 cut(s)	579, 1247
AdeI	CACNNNGTG	1 cut(s)	1743
AfaI	GTAC	6 cut(s)	523, 1697, 1870, 1893, 2020, 2168
AfiI	CCNNNNNNNGG	9 cut(s)	203, 204, 531, 577, 631, 1373, 1374, 1971, 2010
AflIII	ACRYGT	2 cut(s)	370, 486
AgsI	TTSAA	5 cut(s)	99, 431, 1003, 1633, 2339
AjnI	CCWGG	1 cut(s)	2256
AjuI	GAANNNNNNNTTGG	2 cut(s)	1599, 1631
Alw21I	GWGCWC	1 cut(s)	1748
Alw26I	GTCTC	2 cut(s)	1165, 2239
AlwI	GGATC	5 cut(s)	469, 1070, 1083, 1172, 2168
AlwNI	CAGNNNCTG	1 cut(s)	134
AoxI	GGCC	8 cut(s)	178, 232, 596, 664, 1088, 1726, 1832, 2128
ApeKI	GCWGC	4 cut(s)	334, 554, 589, 2040
ApoI	RAATTY	2 cut(s)	409, 1585
Asp700I	GAANNNNTTC	2 cut(s)	1683, 2130
AspA2I	CCTAGG	1 cut(s)	1594
AspLEI	GCGC	2 cut(s)	1879, 2326
AspS9I	GGNCC	4 cut(s)	179, 597, 622, 2089
AsuC2I	CCSGG	2 cut(s)	1421, 2393
AsuHPI	GGTGA	8 cut(s)	456, 746, 770, 1020, 1356, 1582, 1610, 1721
AvaII	GGWCC	2 cut(s)	622, 2089
AvrII	CCTAGG	1 cut(s)	1594
BalI	TGGCCA	1 cut(s)	1090
BamHI	GGATCC	1 cut(s)	1075
BanII	GRGCYC	1 cut(s)	1486
BauI	CACGAG	2 cut(s)	866, 2216
BbsI	GAAGAC	4 cut(s)	567, 806, 1206, 1926
Bbv12I	GWGCWC	1 cut(s)	1748
BbvCI	CCTCAGC	2 cut(s)	127, 387
BbvI	GCAGC	4 cut(s)	321, 541, 601, 2052
BceAI	ACGGC	2 cut(s)	912, 1847
BciT130I	CCWGG	1 cut(s)	2258
BclI	TGATCA	2 cut(s)	1144, 2289
BcnI	CCSGG	2 cut(s)	1421, 2393
BcoDI	GTCTC	2 cut(s)	1165, 2239
BfaI	CTAG	4 cut(s)	1595, 1670, 1845, 2045
BfmI	CTRYAG	2 cut(s)	910, 1247
BfoI	RGCGCY	1 cut(s)	2327
BfuAI	ACCTGC	1 cut(s)	2100
BglII	AGATCT	1 cut(s)	306
BisI	GCNGC	5 cut(s)	335, 555, 590, 2041, 2111
BlnI	CCTAGG	1 cut(s)	1594
BlsI	GCNGC	5 cut(s)	336, 556, 591, 2042, 2112
BmcAI	AGTACT	1 cut(s)	1870
Bme1390I	CCNGG	3 cut(s)	1421, 2258, 2393
Bme18I	GGWCC	2 cut(s)	622, 2089
BmgT120I	GGNCC	4 cut(s)	179, 597, 622, 2089
BmiI	GGNNCC	3 cut(s)	1077, 1255, 2090
BmrFI	CCNGG	3 cut(s)	1421, 2258, 2393
BmrI	ACTGGG	3 cut(s)	846, 1950, 1980
BmsI	GCATC	6 cut(s)	270, 727, 978, 1097, 1401, 1448
BmuI	ACTGGG	3 cut(s)	846, 1950, 1980
BpiI	GAAGAC	4 cut(s)	567, 806, 1206, 1926
BplI	GAGNNNNNCTC	2 cut(s)	586, 618
BpmI	CTGGAG	3 cut(s)	176, 1302, 2126
Bpu10I	CCTNAGC	3 cut(s)	127, 387, 773
BpuEI	CTTGAG	1 cut(s)	588
BpuMI	CCSGG	2 cut(s)	1421, 2393
BsaBI	GATNNNNATC	1 cut(s)	2279
BsaI	GGTCTC	1 cut(s)	2239
BsaJI	CCNNGG	8 cut(s)	273, 526, 671, 1166, 1419, 1594, 2210, 2256
BsaXI	ACNNNNNCTCC	2 cut(s)	629, 659
Bsc4I	CCNNNNNNNGG	9 cut(s)	203, 204, 531, 577, 631, 1373, 1374, 1971, 2010
Bse118I	RCCGGY	2 cut(s)	1724, 2326
Bse1I	ACTGG	8 cut(s)	519, 697, 852, 980, 1945, 1975, 2004, 2109
Bse3DI	GCAATG	2 cut(s)	61, 2233
Bse8I	GATNNNNATC	1 cut(s)	2279
BseBI	CCWGG	1 cut(s)	2258
BseDI	CCNNGG	8 cut(s)	273, 526, 671, 1166, 1419, 1594, 2210, 2256
BseJI	GATNNNNATC	1 cut(s)	2279
BseLI	CCNNNNNNNGG	9 cut(s)	203, 204, 531, 577, 631, 1373, 1374, 1971, 2010
BseMI	GCAATG	2 cut(s)	61, 2233
BseMII	CTCAG	5 cut(s)	141, 317, 384, 401, 2009
BseNI	ACTGG	8 cut(s)	519, 697, 852, 980, 1945, 1975, 2004, 2109
BseRI	GAGGAG	2 cut(s)	244, 247
BseXI	GCAGC	4 cut(s)	321, 541, 601, 2052
BseYI	CCCAGC	1 cut(s)	1289
BsgI	GTGCAG	1 cut(s)	1820
Bsh1236I	CGCG	1 cut(s)	2069
BshFI	GGCC	8 cut(s)	180, 234, 598, 666, 1090, 1728, 1834, 2130
BsiHKAI	GWGCWC	1 cut(s)	1748
BsiSI	CCGG	5 cut(s)	1421, 1725, 2327, 2369, 2392
BslFI	GGGAC	3 cut(s)	1006, 1945, 2102
BslI	CCNNNNNNNGG	9 cut(s)	203, 204, 531, 577, 631, 1373, 1374, 1971, 2010
BsmAI	GTCTC	2 cut(s)	1165, 2239
BsmFI	GGGAC	3 cut(s)	1006, 1945, 2102
BsmI	GAATGC	1 cut(s)	49
BsnI	GGCC	8 cut(s)	180, 234, 598, 666, 1090, 1728, 1834, 2130
Bso31I	GGTCTC	1 cut(s)	2239
Bsp1286I	GDGCHC	2 cut(s)	1486, 1748
Bsp1407I	TGTACA	2 cut(s)	2018, 2166
Bsp19I	CCATGG	3 cut(s)	273, 1166, 2210
Bsp68I	TCGCGA	1 cut(s)	2069
BspACI	CCGC	8 cut(s)	1237, 1393, 1511, 1646, 1884, 2011, 2110, 2158
BspANI	GGCC	8 cut(s)	180, 234, 598, 666, 1090, 1728, 1834, 2130
BspCNI	CTCAG	5 cut(s)	140, 316, 385, 400, 2008
BspFNI	CGCG	1 cut(s)	2069
BspLI	GGNNCC	3 cut(s)	1077, 1255, 2090
BspMAI	CTGCAG	1 cut(s)	1251
BspMI	ACCTGC	1 cut(s)	2100
BspPI	GGATC	5 cut(s)	469, 1070, 1083, 1172, 2168
BspQI	GCTCTTC	1 cut(s)	2047
BspTNI	GGTCTC	1 cut(s)	2239
BsrBI	CCGCTC	2 cut(s)	1395, 2110
BsrDI	GCAATG	2 cut(s)	61, 2233
BsrFI	RCCGGY	2 cut(s)	1724, 2326
BsrGI	TGTACA	2 cut(s)	2018, 2166
BsrI	ACTGG	8 cut(s)	519, 697, 852, 980, 1945, 1975, 2004, 2109
BssAI	RCCGGY	2 cut(s)	1724, 2326
BssECI	CCNNGG	8 cut(s)	273, 526, 671, 1166, 1419, 1594, 2210, 2256
BssSI	CACGAG	2 cut(s)	866, 2216
BssT1I	CCWWGG	5 cut(s)	273, 671, 1166, 1594, 2210
Bst2BI	CACGAG	2 cut(s)	866, 2216
Bst2UI	CCWGG	1 cut(s)	2258
Bst4CI	ACNGT	6 cut(s)	566, 914, 1387, 1798, 1936, 2386
Bst6I	CTCTTC	3 cut(s)	100, 426, 2047
BstAPI	GCANNNNNTGC	1 cut(s)	1445
BstAUI	TGTACA	2 cut(s)	2018, 2166
BstDEI	CTNAG	8 cut(s)	127, 303, 387, 393, 773, 1326, 1430, 1995
BstDSI	CCRYGG	4 cut(s)	273, 526, 1166, 2210
BstFNI	CGCG	1 cut(s)	2069
BstH2I	RGCGCY	1 cut(s)	2327
BstHHI	GCGC	2 cut(s)	1879, 2326
BstMAI	GTCTC	2 cut(s)	1165, 2239
BstMWI	GCNNNNNNNGC	9 cut(s)	137, 388, 595, 682, 1286, 1445, 1883, 2127, 2332
BstNI	CCWGG	1 cut(s)	2258
BstNSI	RCATGY	6 cut(s)	170, 174, 374, 490, 898, 1418
BstSCI	CCNGG	3 cut(s)	1419, 2256, 2391
BstSFI	CTRYAG	2 cut(s)	910, 1247
BstUI	CGCG	1 cut(s)	2069
BstV1I	GCAGC	4 cut(s)	321, 541, 601, 2052
BstV2I	GAAGAC	4 cut(s)	567, 806, 1206, 1926
BstX2I	RGATCY	3 cut(s)	306, 1075, 2160
BstYI	RGATCY	3 cut(s)	306, 1075, 2160
BsuRI	GGCC	8 cut(s)	180, 234, 598, 666, 1090, 1728, 1834, 2130
BtgI	CCRYGG	4 cut(s)	273, 526, 1166, 2210
BtgZI	GCGATG	2 cut(s)	955, 2310
BtsI	GCAGTG	2 cut(s)	30, 1275
BtsIMutI	CAGTG	5 cut(s)	30, 859, 987, 1275, 2169
BtuMI	TCGCGA	1 cut(s)	2069
BveI	ACCTGC	1 cut(s)	2100
CaiI	CAGNNNCTG	1 cut(s)	134
CfoI	GCGC	2 cut(s)	1879, 2326
Cfr10I	RCCGGY	2 cut(s)	1724, 2326
Cfr13I	GGNCC	4 cut(s)	179, 597, 622, 2089
Csp6I	GTAC	6 cut(s)	522, 1696, 1869, 1892, 2019, 2167
CviQI	GTAC	6 cut(s)	522, 1696, 1869, 1892, 2019, 2167
DdeI	CTNAG	8 cut(s)	127, 303, 387, 393, 773, 1326, 1430, 1995
DraIII	CACNNNGTG	1 cut(s)	1743
EaeI	YGGCCR	3 cut(s)	1088, 1726, 1832
Eam1104I	CTCTTC	3 cut(s)	100, 426, 2047
EarI	CTCTTC	3 cut(s)	100, 426, 2047
EciI	GGCGGA	1 cut(s)	2173
Eco130I	CCWWGG	5 cut(s)	273, 671, 1166, 1594, 2210
Eco147I	AGGCCT	1 cut(s)	2130
Eco24I	GRGCYC	1 cut(s)	1486
Eco31I	GGTCTC	1 cut(s)	2239
Eco47I	GGWCC	2 cut(s)	622, 2089
Eco57I	CTGAAG	2 cut(s)	579, 1247
EcoO109I	RGGNCCY	3 cut(s)	597, 622, 2089
EcoRII	CCWGG	1 cut(s)	2256
EcoT14I	CCWWGG	5 cut(s)	273, 671, 1166, 1594, 2210
EcoT22I	ATGCAT	2 cut(s)	172, 1416
EcoT38I	GRGCYC	1 cut(s)	1486
ErhI	CCWWGG	5 cut(s)	273, 671, 1166, 1594, 2210
FalI	AAGNNNNNCTT	2 cut(s)	2118, 2150
FaqI	GGGAC	3 cut(s)	1006, 1945, 2102
FauI	CCCGC	1 cut(s)	1891
FbaI	TGATCA	2 cut(s)	1144, 2289
FblI	GTMKAC	2 cut(s)	219, 363
Fnu4HI	GCNGC	5 cut(s)	335, 555, 590, 2041, 2111
FriOI	GRGCYC	1 cut(s)	1486
Fsp4HI	GCNGC	5 cut(s)	335, 555, 590, 2041, 2111
FspBI	CTAG	4 cut(s)	1595, 1670, 1845, 2045
GlaI	GCGC	2 cut(s)	1878, 2325
GluI	GCNGC	5 cut(s)	335, 555, 590, 2041, 2111
GsaI	CCCAGC	1 cut(s)	1293
GsuI	CTGGAG	3 cut(s)	176, 1302, 2126
HaeII	RGCGCY	1 cut(s)	2327
HaeIII	GGCC	8 cut(s)	180, 234, 598, 666, 1090, 1728, 1834, 2130
HapII	CCGG	5 cut(s)	1421, 1725, 2327, 2369, 2392
HhaI	GCGC	2 cut(s)	1879, 2326
Hin6I	GCGC	2 cut(s)	1877, 2324
HinP1I	GCGC	2 cut(s)	1877, 2324
HincII	GTYRAC	1 cut(s)	1572
HindII	GTYRAC	1 cut(s)	1572
HpaII	CCGG	5 cut(s)	1421, 1725, 2327, 2369, 2392
HphI	GGTGA	8 cut(s)	456, 746, 770, 1020, 1356, 1582, 1610, 1721
Hpy166II	GTNNAC	8 cut(s)	220, 364, 1390, 1572, 1717, 1949, 2029, 2169
Hpy188III	TCNNGA	8 cut(s)	155, 605, 818, 1151, 1343, 1547, 2068, 2138
Hpy8I	GTNNAC	8 cut(s)	220, 364, 1390, 1572, 1717, 1949, 2029, 2169
HpyAV	CCTTC	8 cut(s)	410, 635, 1191, 1450, 1694, 2120, 2141, 2144
HpyCH4III	ACNGT	6 cut(s)	566, 914, 1387, 1798, 1936, 2386
HpyCH4IV	ACGT	2 cut(s)	1635, 1713
HpyF10VI	GCNNNNNNNGC	9 cut(s)	137, 388, 595, 682, 1286, 1445, 1883, 2127, 2332
HpyF3I	CTNAG	8 cut(s)	127, 303, 387, 393, 773, 1326, 1430, 1995
HpySE526I	ACGT	2 cut(s)	1635, 1713
HspAI	GCGC	2 cut(s)	1877, 2324
KroI	GCCGGC	1 cut(s)	1724
KroNI	GCCGGC	1 cut(s)	1726
Ksp22I	TGATCA	2 cut(s)	1144, 2289
LguI	GCTCTTC	1 cut(s)	2047
LmnI	GCTCC	4 cut(s)	136, 157, 241, 2107
Lsp1109I	GCAGC	4 cut(s)	321, 541, 601, 2052
LweI	GCATC	6 cut(s)	270, 727, 978, 1097, 1401, 1448
MaeI	CTAG	4 cut(s)	1595, 1670, 1845, 2045
MaeII	ACGT	2 cut(s)	1635, 1713
MaeIII	GTNAC	6 cut(s)	373, 982, 1054, 1709, 1810, 1936
MbiI	CCGCTC	2 cut(s)	1395, 2110
MflI	RGATCY	3 cut(s)	306, 1075, 2160
MhlI	GDGCHC	2 cut(s)	1486, 1748
MlsI	TGGCCA	1 cut(s)	1090
MluCI	AATT	7 cut(s)	6, 118, 147, 409, 783, 808, 1585
MluNI	TGGCCA	1 cut(s)	1090
MlyI	GAGTC	2 cut(s)	1167, 1523
MmeI	TCCRAC	2 cut(s)	1635, 1807
Mox20I	TGGCCA	1 cut(s)	1090
Mph1103I	ATGCAT	2 cut(s)	172, 1416
MroNI	GCCGGC	1 cut(s)	1724
MroXI	GAANNNNTTC	2 cut(s)	1683, 2130
MscI	TGGCCA	1 cut(s)	1090
MseI	TTAA	2 cut(s)	1296, 1317
MslI	CAYNNNNRTG	3 cut(s)	171, 278, 2285
Msp20I	TGGCCA	1 cut(s)	1090
MspA1I	CMGCKG	1 cut(s)	334
MspI	CCGG	5 cut(s)	1421, 1725, 2327, 2369, 2392
MspR9I	CCNGG	3 cut(s)	1421, 2258, 2393
Mva1269I	GAATGC	1 cut(s)	49
MvaI	CCWGG	1 cut(s)	2258
MvnI	CGCG	1 cut(s)	2069
MwoI	GCNNNNNNNGC	9 cut(s)	137, 388, 595, 682, 1286, 1445, 1883, 2127, 2332
NaeI	GCCGGC	1 cut(s)	1726
NciI	CCSGG	2 cut(s)	1421, 2393
NcoI	CCATGG	3 cut(s)	273, 1166, 2210
NgoMIV	GCCGGC	1 cut(s)	1724
NlaIV	GGNNCC	3 cut(s)	1077, 1255, 2090
NmuCI	GTSAC	4 cut(s)	373, 982, 1054, 1709
NruI	TCGCGA	1 cut(s)	2069
NsiI	ATGCAT	2 cut(s)	172, 1416
NspI	RCATGY	6 cut(s)	170, 174, 374, 490, 898, 1418
PaeI	GCATGC	2 cut(s)	170, 1418
PceI	AGGCCT	1 cut(s)	2130
PciI	ACATGT	2 cut(s)	370, 486
PciSI	GCTCTTC	1 cut(s)	2047
PctI	GAATGC	1 cut(s)	49
PdiI	GCCGGC	1 cut(s)	1726
PdmI	GAANNNNTTC	2 cut(s)	1683, 2130
PkrI	GCNGC	5 cut(s)	336, 556, 591, 2042, 2112
PleI	GAGTC	2 cut(s)	1166, 1522
PpsI	GAGTC	2 cut(s)	1166, 1522
PpuMI	RGGWCCY	2 cut(s)	622, 2089
PscI	ACATGT	2 cut(s)	370, 486
Psp1406I	AACGTT	1 cut(s)	1635
Psp5II	RGGWCCY	2 cut(s)	622, 2089
Psp6I	CCWGG	1 cut(s)	2256
PspFI	CCCAGC	1 cut(s)	1289
PspGI	CCWGG	1 cut(s)	2256
PspN4I	GGNNCC	3 cut(s)	1077, 1255, 2090
PspPI	GGNCC	4 cut(s)	179, 597, 622, 2089
PspPPI	RGGWCCY	2 cut(s)	622, 2089
PstI	CTGCAG	1 cut(s)	1251
PstNI	CAGNNNCTG	1 cut(s)	134
PsuI	RGATCY	3 cut(s)	306, 1075, 2160
PvuII	CAGCTG	1 cut(s)	334
RruI	TCGCGA	1 cut(s)	2069
RsaI	GTAC	6 cut(s)	523, 1697, 1870, 1893, 2020, 2168
RsaNI	GTAC	6 cut(s)	522, 1696, 1869, 1892, 2019, 2167
RseI	CAYNNNNRTG	3 cut(s)	171, 278, 2285
SapI	GCTCTTC	1 cut(s)	2047
SaqAI	TTAA	2 cut(s)	1296, 1317
SatI	GCNGC	5 cut(s)	335, 555, 590, 2041, 2111
Sau96I	GGNCC	4 cut(s)	179, 597, 622, 2089
ScaI	AGTACT	1 cut(s)	1870
SchI	GAGTC	2 cut(s)	1167, 1523
ScrFI	CCNGG	3 cut(s)	1421, 2258, 2393
SduI	GDGCHC	2 cut(s)	1486, 1748
SfaNI	GCATC	6 cut(s)	270, 727, 978, 1097, 1401, 1448
SfcI	CTRYAG	2 cut(s)	910, 1247
SinI	GGWCC	2 cut(s)	622, 2089
SmiMI	CAYNNNNRTG	3 cut(s)	171, 278, 2285
SmlI	CTYRAG	1 cut(s)	603
SmoI	CTYRAG	1 cut(s)	603
SphI	GCATGC	2 cut(s)	170, 1418
Sse9I	AATT	7 cut(s)	6, 118, 147, 409, 783, 808, 1585
SseBI	AGGCCT	1 cut(s)	2130
SsiI	CCGC	8 cut(s)	1237, 1393, 1511, 1646, 1884, 2011, 2110, 2158
SspMI	CTAG	4 cut(s)	1595, 1670, 1845, 2045
StuI	AGGCCT	1 cut(s)	2130
StyD4I	CCNGG	3 cut(s)	1419, 2256, 2391
StyI	CCWWGG	5 cut(s)	273, 671, 1166, 1594, 2210
TaaI	ACNGT	6 cut(s)	566, 914, 1387, 1798, 1936, 2386
TaiI	ACGT	2 cut(s)	1638, 1716
TaqI	TCGA	5 cut(s)	799, 1014, 1084, 1156, 2137
TasI	AATT	7 cut(s)	6, 118, 147, 409, 783, 808, 1585
TatI	WGTACW	4 cut(s)	1695, 1868, 2018, 2166
TauI	GCSGC	1 cut(s)	2113
Tru1I	TTAA	2 cut(s)	1296, 1317
Tru9I	TTAA	2 cut(s)	1296, 1317
TscAI	CASTG	5 cut(s)	30, 859, 987, 1282, 2176
TseFI	GTSAC	4 cut(s)	373, 982, 1054, 1709
TseI	GCWGC	4 cut(s)	334, 554, 589, 2040
Tsp45I	GTSAC	4 cut(s)	373, 982, 1054, 1709
TspGWI	ACGGA	4 cut(s)	543, 780, 1068, 2347
TspRI	CASTG	5 cut(s)	30, 859, 987, 1282, 2176
VpaK11BI	GGWCC	2 cut(s)	622, 2089
XapI	RAATTY	2 cut(s)	409, 1585
XceI	RCATGY	6 cut(s)	170, 174, 374, 490, 898, 1418
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1098
XmaJI	CCTAGG	1 cut(s)	1594
XmiI	GTMKAC	2 cut(s)	219, 363
XmnI	GAANNNNTTC	2 cut(s)	1683, 2130
XspI	CTAG	4 cut(s)	1595, 1670, 1845, 2045
ZrmI	AGTACT	1 cut(s)	1870
Zsp2I	ATGCAT	2 cut(s)	172, 1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.