Rorug05G0327400

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006152
Physical Location & Seq
Forward (+)
39380302 .. 39382623
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0327400.1

Sequence Viewer

Length: 1167 bp
ATGCCGGCGACGTTTCGTCGCTTTCTCACCGCTACTCTCAGACGCCCCAATATCCTCTCTCCTCAACGCCCGCCTCTCTCCTCCCGCCACGTAATCCCTGACCCTTCATTTCCGTTCACCCTCCCGTCACTCTATCACCGCCTCCTATCCTCCTCCGCCGCCCAAAACCCCCCGGACTCATTCCGAACCCGAACCCCACTGGAGAAGCAATTCGAAACATGGGTCCAGAAACTCAAACCCGGATTCGGCCCACCCGACGTCGACGAGGCCCTGAAAGCCCAATCGGATCCCGACCTCGCGCTCGACATTTTCCGATGGACGGCCCAGCAGCGCAACTACAAGCACAACCACTCCACCTACCTCACCATGATCAAAATCCTAATCAACGGCCGCCGCTACCGCCACGCCGAAACCCTACTTGAGGAGGTGGTCGCCGGAGCTTGTGAGATAAGTGTCCCGCTTTATAACTCCATTATTCGATTCTGTTGTGGAAGAAAGATGCTCTTCAATCGCGCTTTTGATGTGTACAAGAAAATGTTGAATTCGGAGAATTGTAAGCCCACATTGGAAACCTATGCATTGCTGCTTAATTCATTGCTCAGGAGGTTTAATAATATCACTGTTTGCCATGTGTATTTGCGGTCGGTGAGGTCGTTGTCGAAGCAAATGAAGGCATCCGGTGTGATACCGGATACTTTTGTGTTGAATATGATCATCAAGGCTTATTCCAAGTGTTTGGAAGTTGATGAGGCACTTCAGGTTTTTCGGGAAATGGGGTTGTATGGATGTGAGCCGAATGGTTATACTTATGGTTACATTGCCAAAGGGTTGTGTGAGAAGGGGAGGGTAGGTCAGGGTTTAGGGTTTTACAAGGAAATGAGAGGGAAGGGTTTGGTGCCTAGCAGTAGTACTTATATGGTTGTAATTTGCAGTCTTGCTCTGGAGCGGAGATATGAGGATGCGAGTAATGTTGTGTTTGATATGTTGAGTAATTCTATGTCTCCTGATTTGTTAACTTACAAAACTTTATTGGAAGGATTGTGCCGAGATGGAAAAGGTGAGGAGGCATTTGATCTACTAGAGACTTTTAGGAGCAGGGATAGAGCCATGAATGAAAAGACATACAAGACATTGTTAAATGCATTGCATTTTGTAAATAGGGAGTAG

Protein Analysis

388

Amino Acids

44.41

Weight (kDa)

9.28

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 152 - 200 1.3e-06 PPR repeat family
PPR_long PF17177 163 - 286 8.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 230 - 278 8.8e-13 PPR repeat family
PPR_long PF17177 286 - 381 1.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 300 - 349 6.6e-08 PPR repeat family
PPR_1 PF12854 333 - 361 7e-08 PPR repeat
PPR_2 PF13041 335 - 382 6e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 895
AccBSI CCGCTC 1 cut(s) 946
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 299, 513
AclWI GGATC 2 cut(s) 281, 294
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 1 cut(s) 541
AcuI CTGAAG 1 cut(s) 740
AcyI GRCGYC 2 cut(s) 43, 258
AfaI GTAC 2 cut(s) 527, 910
AfiI CCNNNNNNNGG 3 cut(s) 245, 319, 421
AgsI TTSAA 3 cut(s) 508, 541, 706
AhdI GACNNNNNGTC 1 cut(s) 15
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw26I GTCTC 2 cut(s) 1005, 1076
AlwI GGATC 2 cut(s) 281, 294
AoxI GGCC 4 cut(s) 247, 267, 321, 388
ApeKI GCWGC 2 cut(s) 328, 583
ApoI RAATTY 1 cut(s) 541
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 301, 333, 515
AspS9I GGNCC 4 cut(s) 223, 248, 268, 322
AsuC2I CCSGG 2 cut(s) 173, 240
AsuHPI GGTGA 6 cut(s) 19, 109, 128, 355, 658, 1070
AsuII TTCGAA 1 cut(s) 213
AvaII GGWCC 1 cut(s) 223
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BamHI GGATCC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 895
BbvI GCAGC 2 cut(s) 340, 570
BccI CCATC 2 cut(s) 309, 1043
BceAI ACGGC 2 cut(s) 336, 403
BciVI GTATCC 1 cut(s) 685
BclI TGATCA 2 cut(s) 369, 711
BcnI CCSGG 2 cut(s) 173, 240
BcoDI GTCTC 2 cut(s) 1005, 1076
BfaI CTAG 2 cut(s) 900, 1079
BfuI GTATCC 1 cut(s) 685
BisI GCNGC 5 cut(s) 159, 329, 391, 394, 584
BlsI GCNGC 5 cut(s) 160, 330, 392, 395, 585
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 2 cut(s) 173, 240
Bme18I GGWCC 1 cut(s) 223
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 4 cut(s) 223, 248, 268, 322
BmiI GGNNCC 3 cut(s) 224, 288, 897
BmrFI CCNGG 2 cut(s) 173, 240
BmsI GCATC 3 cut(s) 489, 683, 949
BpmI CTGGAG 2 cut(s) 221, 962
Bpu10I CCTNAGC 1 cut(s) 599
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 2 cut(s) 173, 240
BsaAI YACGTR 1 cut(s) 91
BsaBI GATNNNNATC 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 43, 258
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 2 cut(s) 677, 688
BsaXI ACNNNNNCTCC 4 cut(s) 335, 365, 835, 865
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 319, 421
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 204
Bse3DI GCAATG 4 cut(s) 578, 593, 816, 1142
Bse8I GATNNNNATC 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 674, 791, 964
BseJI GATNNNNATC 1 cut(s) 374
BseLI CCNNNNNNNGG 3 cut(s) 245, 319, 421
BseMI GCAATG 4 cut(s) 578, 593, 816, 1142
BseMII CTCAG 2 cut(s) 52, 613
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 5 cut(s) 51, 70, 142, 437, 1076
BseX3I CGGCCG 1 cut(s) 388
BseXI GCAGC 2 cut(s) 340, 570
BseYI CCCAGC 1 cut(s) 324
Bsh1236I CGCG 2 cut(s) 299, 513
Bsh1285I CGRYCG 2 cut(s) 391, 644
BshFI GGCC 4 cut(s) 249, 269, 323, 390
BshNI GGYRCC 1 cut(s) 895
BsiEI CGRYCG 2 cut(s) 391, 644
BsiSI CCGG 6 cut(s) 5, 173, 240, 435, 678, 689
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 3 cut(s) 245, 319, 421
BsmAI GTCTC 2 cut(s) 1005, 1076
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 4 cut(s) 249, 269, 323, 390
Bsp119I TTCGAA 1 cut(s) 213
Bsp1407I TGTACA 1 cut(s) 525
Bsp143I GATC 4 cut(s) 286, 369, 711, 1072
BspANI GGCC 4 cut(s) 249, 269, 323, 390
BspCNI CTCAG 2 cut(s) 51, 612
BspFNI CGCG 2 cut(s) 299, 513
BspLI GGNNCC 3 cut(s) 224, 288, 897
BspPI GGATC 2 cut(s) 281, 294
BspQI GCTCTTC 1 cut(s) 509
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 895
BsrBI CCGCTC 1 cut(s) 946
BsrDI GCAATG 4 cut(s) 578, 593, 816, 1142
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 286, 369, 711, 1072
BssNI GRCGYC 2 cut(s) 43, 258
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 1 cut(s) 509
BstACI GRCGYC 2 cut(s) 43, 258
BstAUI TGTACA 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 91
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 6, 71
BstDEI CTNAG 2 cut(s) 38, 599
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 3 cut(s) 674, 791, 964
BstFNI CGCG 2 cut(s) 299, 513
BstHHI GCGC 3 cut(s) 301, 333, 515
BstKTI GATC 4 cut(s) 289, 372, 714, 1075
BstMAI GTCTC 2 cut(s) 1005, 1076
BstMBI GATC 4 cut(s) 286, 369, 711, 1072
BstMCI CGRYCG 2 cut(s) 391, 644
BstMWI GCNNNNNNNGC 2 cut(s) 275, 399
BstSCI CCNGG 2 cut(s) 171, 238
BstUI CGCG 2 cut(s) 299, 513
BstV1I GCAGC 2 cut(s) 340, 570
BstX2I RGATCY 1 cut(s) 286
BstXI CCANNNNNNTGG 1 cut(s) 736
BstYI RGATCY 1 cut(s) 286
BstZI CGGCCG 1 cut(s) 388
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 4 cut(s) 249, 269, 323, 390
BtsCI GGATG 3 cut(s) 674, 791, 964
BtsIMutI CAGTG 2 cut(s) 197, 618
Cac8I GCNNGC 2 cut(s) 6, 71
CfoI GCGC 3 cut(s) 301, 333, 515
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 4 cut(s) 223, 248, 268, 322
CseI GACGC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 526, 909
CviAII CATG 4 cut(s) 219, 367, 629, 1108
CviQI GTAC 2 cut(s) 526, 909
DdeI CTNAG 2 cut(s) 38, 599
DpnI GATC 4 cut(s) 288, 371, 713, 1074
DpnII GATC 4 cut(s) 286, 369, 711, 1072
DriI GACNNNNNGTC 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 388
EagI CGGCCG 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 509
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 509
EciI GGCGGA 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 223
Eco52I CGGCCG 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 740
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 541
EcoT22I ATGCAT 2 cut(s) 580, 1144
FaeI CATG 4 cut(s) 222, 370, 632, 1111
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 1 cut(s) 440
FatI CATG 4 cut(s) 218, 366, 628, 1107
FauI CCCGC 3 cut(s) 78, 92, 465
FbaI TGATCA 2 cut(s) 369, 711
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 159, 329, 391, 394, 584
FokI GGATG 3 cut(s) 661, 798, 971
Fsp4HI GCNGC 5 cut(s) 159, 329, 391, 394, 584
FspBI CTAG 2 cut(s) 900, 1079
GlaI GCGC 3 cut(s) 300, 332, 514
GluI GCNGC 5 cut(s) 159, 329, 391, 394, 584
GsaI CCCAGC 1 cut(s) 328
GsuI CTGGAG 2 cut(s) 221, 962
HaeIII GGCC 4 cut(s) 249, 269, 323, 390
HapII CCGG 6 cut(s) 5, 173, 240, 435, 678, 689
HgaI GACGC 1 cut(s) 51
HhaI GCGC 3 cut(s) 301, 333, 515
Hin1I GRCGYC 2 cut(s) 43, 258
Hin1II CATG 4 cut(s) 222, 370, 632, 1111
Hin6I GCGC 3 cut(s) 299, 331, 513
HinP1I GCGC 3 cut(s) 299, 331, 513
HincII GTYRAC 2 cut(s) 262, 1014
HindII GTYRAC 2 cut(s) 262, 1014
HinfI GANTC 3 cut(s) 176, 243, 480
HpaI GTTAAC 1 cut(s) 1014
HpaII CCGG 6 cut(s) 5, 173, 240, 435, 678, 689
HphI GGTGA 6 cut(s) 19, 109, 128, 355, 658, 1070
Hpy166II GTNNAC 4 cut(s) 117, 262, 526, 1014
Hpy188I TCNGA 5 cut(s) 41, 185, 286, 314, 547
Hpy188III TCNNGA 6 cut(s) 226, 290, 601, 767, 941, 1004
Hpy8I GTNNAC 4 cut(s) 117, 262, 526, 1014
Hpy99I CGWCG 5 cut(s) 13, 21, 260, 263, 266
HpyAV CCTTC 5 cut(s) 114, 664, 832, 880, 1028
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 3 cut(s) 11, 90, 258
HpyCH4V TGCA 4 cut(s) 578, 930, 1142, 1147
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 399
HpyF3I CTNAG 2 cut(s) 38, 599
HpySE526I ACGT 3 cut(s) 11, 90, 258
Hsp92I GRCGYC 2 cut(s) 43, 258
Hsp92II CATG 4 cut(s) 222, 370, 632, 1111
HspAI GCGC 3 cut(s) 299, 331, 513
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 369, 711
KspAI GTTAAC 1 cut(s) 1014
Kzo9I GATC 4 cut(s) 286, 369, 711, 1072
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 3 cut(s) 437, 943, 1092
Lsp1109I GCAGC 2 cut(s) 340, 570
LweI GCATC 3 cut(s) 489, 683, 949
MaeI CTAG 2 cut(s) 900, 1079
MaeII ACGT 3 cut(s) 11, 90, 258
MaeIII GTNAC 2 cut(s) 126, 812
MalI GATC 4 cut(s) 288, 371, 713, 1074
MbiI CCGCTC 1 cut(s) 946
MboI GATC 4 cut(s) 286, 369, 711, 1072
MboII GAAGA 2 cut(s) 496, 504
MflI RGATCY 1 cut(s) 286
MluCI AATT 6 cut(s) 209, 541, 550, 589, 924, 991
MlyI GAGTC 1 cut(s) 170
Mph1103I ATGCAT 2 cut(s) 580, 1144
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 4 cut(s) 588, 609, 1013, 1136
MspI CCGG 6 cut(s) 5, 173, 240, 435, 678, 689
MspR9I CCNGG 2 cut(s) 173, 240
MvnI CGCG 2 cut(s) 299, 513
MwoI GCNNNNNNNGC 2 cut(s) 275, 399
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 173, 240
NdeII GATC 4 cut(s) 286, 369, 711, 1072
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 4 cut(s) 222, 370, 632, 1111
NlaIV GGNNCC 3 cut(s) 224, 288, 897
NmeAIII GCCGAG 1 cut(s) 1070
NmuCI GTSAC 1 cut(s) 126
NsiI ATGCAT 2 cut(s) 580, 1144
NspV TTCGAA 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 509
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 650
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 2 cut(s) 243, 480
PkrI GCNGC 5 cut(s) 160, 330, 392, 395, 585
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Ppu21I YACGTR 1 cut(s) 91
PsiI TTATAA 1 cut(s) 465
PspFI CCCAGC 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 224, 288, 897
PspPI GGNCC 4 cut(s) 223, 248, 268, 322
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 2 cut(s) 527, 910
RsaNI GTAC 2 cut(s) 526, 909
SalI GTCGAC 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 4 cut(s) 588, 609, 1013, 1136
SatI GCNGC 5 cut(s) 159, 329, 391, 394, 584
Sau3AI GATC 4 cut(s) 286, 369, 711, 1072
Sau96I GGNCC 4 cut(s) 223, 248, 268, 322
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 173, 240
SfaNI GCATC 3 cut(s) 489, 683, 949
SfuI TTCGAA 1 cut(s) 213
SgrDI CGTCGACG 1 cut(s) 260
SinI GGWCC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 6 cut(s) 209, 541, 550, 589, 924, 991
SspMI CTAG 2 cut(s) 900, 1079
StyD4I CCNGG 2 cut(s) 171, 238
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 3 cut(s) 14, 93, 261
TaqI TCGA 5 cut(s) 213, 261, 303, 478, 659
TasI AATT 6 cut(s) 209, 541, 550, 589, 924, 991
TatI WGTACW 2 cut(s) 525, 908
TauI GCSGC 3 cut(s) 161, 393, 396
TfiI GAWTC 2 cut(s) 243, 480
Tru1I TTAA 4 cut(s) 588, 609, 1013, 1136
Tru9I TTAA 4 cut(s) 588, 609, 1013, 1136
TscAI CASTG 2 cut(s) 204, 625
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 2 cut(s) 328, 583
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 5 cut(s) 96, 582, 683, 1124, 1128
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 2 cut(s) 204, 625
VpaK11BI GGWCC 1 cut(s) 223
XagI CCTNNNNNAGG 1 cut(s) 419
XapI RAATTY 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 2 cut(s) 900, 1079
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 910
Zsp2I ATGCAT 2 cut(s) 580, 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.