Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005910

Physical Location & Seq

Genomic Coordinates Forward (+)

12525052 .. 12530824

Gene Structure 5773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1129000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3663 bp

ATGGGCACCCCCAACCCAAACCTAAACCCATCATCCATGCGGGTCCTAGTCCGCCCACCTCCTACACCCACCGCCAAACCCACTTCCAATTCACCCTCCCAAACTCTTCCGCTTCCCTCCTCCGCAGACCCTCCCGCTTCCGACGGTGTTGTCGTCGTGGGCTTCATTGGCCGGAGCCCCGACGACTCTGCCCAACTCATCAACCGGATCCTCGACTCCAACGTGTTCGGGTCGGGCAATCTCGACAAAAGCCTCTTTCTTGAGAAAGAGGAGCTCAGGGACTGGTTTAGGTGGCGAAGAATCAGTTACTTCCATGAACAGAAAAAGGGCATTCTGTTTTTGCAGTTCTGCTCTACCCGGTGCCCCGCCGTGGCTGATGGGTTCTCGGATTCCGGGTCGGGTTTTGACTCTCCAGTGGAAGAGCATGACTTTGGGGACCTTCAGGCGCTGCTCTTCATGTTCTCTGTTTGCCATGTAATAGTATATATTCTGGAGGGTTCACAGTTTGATTCGCAACTCCTGCAAAAGTTTCGAGTGTTACAAGCTGCCAAGCATGCTTTGGCTCCGTTTGTGAGATCCAGAACTGTGCAGCCAACATCATCCAGGCCACCTTCATCGTCATCCTCACGGCCTACCACATCCGCTGCCACCACTAGCAATTCATCTCAAGGTAGAAGTGGTGGCATCTTGACTCGCAATGCTTCTTCCATTTCACTTATGTCAGGTTTAGGTTCCTACACTTCTTTGTTCCCAGGACAGTGTACCCCAGTCACACTGTTTGTTTTTATTGATGATTTCTCTGATGTGCCAAATCCCAGTTCTAATCTGGAGGAGTCGGCAGATACATCCTCACTTAATCAATCGTCTAGTGTGAGCAGTTTAGCAAGGCCAAGCTTGCCTGTTAAGGGTTCAGGTTCAGTGGTTGTGCTTGCCCGCCCTGTGAGTAAATCTGAGGGCAGTTTTCGGAAGAAACTGCATTCATCCCTTGAAGCACAGATTCGTTTCTTAATTAAGAAGTGTAGAACACTCTCAGGTTCTGAAAGTAGTCATGCGGGGTCAAGAAGCGGGGGTTCTTCAAATTCAGCACCTTTATTCTCCCTTGATGCATCAAGGGCTGTCCTATTGTTAGATAGGTCTACGAATCAGAGAGGTGAATCTCTTGAATTTGCCACAGGACTTGTGGAAGATGTTTTGAATGGGAAGGCAACCTCAGATTCTCTTCTGCTTGAAAGTCATGGTCAGAATGCAAACAAAGAGGATATCGTATCAGTCAAGGATTTTATTTACAGACAATCTGATATTCTAAGAGGCAGGGGGGGACCGGTAAATAATTCCAGTAGTGGTTCAACTGCTGGTGTAGGTATGGTTGCTGTTGCTGCAGCTGTTGCCGCTGCATCAGCTGCATCTTCATCTGCTGCATCTTCATCCGCTGCATCTGGAAAGACGATGTCTACTCCTGAACTTCCAAATTTCCAAATTTGGTTATCTTCCAGTCAACAAATTCTTGATGGAGTTCTTTCTGCAAAAGGTGGGTGCTTATATGAAACTGAAATTAGCAAACGAAAACTTCGCCTAAGAAACACTGTTCCACAGCAGGCTGAAGGAGTTTCTTCAAAAGGTACGGATCCTCTAGATTTAGCAGTATCTTGCCTGGAGAACGGTAAAAGGCTAAACACAAAATTTTCAGTTTTGTGGTGTGAAAGGACTCTTCCAGCTGCTAAAGAGGTTTATCTCAAAGACTTGCCTGCTTGTTACCCAACTTCACAGCATGAAGCCTATCTAGAGAAGGCTTTGCAAGCTTTCCATTCAATGGTGAAAGGACATGCAGTGCAACAGTTTGCAAAAAAGTTGGAGGATGAATGCACATCCATCTGGAAATCTGGAAGGCAACTGTGTGATGCTGTTAGTTTGACTGGAAAACCATGTATGCACCAGAGACATGATGTTGAAGCTAGTGAATCACTTTCAGGAGTTCCTGTGAAGCAACATTCCAGTGGATATGTTTTCCTTCACGCATGTTCTTGTGGTCGTTCACGGAAGCTAAGATCTGATCCTTTTGACTTCAAGTCAGCAAACATTACTTTCAATCGCTTCCCAGATTGTGACAAGCACCTTCCCACACTTCAACTGCCTGAAGTAAGCAATACAAGCCCTATTCAACCTTCCTCATGGAATTTGGTCCGCATTGGTGCTGCAAAGTACTATGAACCTTCTAAAGGCTTGCTTCAAAGCGGGTTTTCTTCCACTCAGAAGTTTCTTTTGAAATGGATAATACATTTGGGAAAGCAGAAAAGCCCGAATGAGTTACCTACCAGTGCAGTGCAACAAGGTTCAGTAGTTAGGTCAGAATCCAACCTCAAGTTTGAATCTAAAGCTGGTGTGCAGTTGTACACGGGGGAGTTAAAATCTGGAGTAGCAAGTCATAGAAAACCTGCAGAGGACTTAGTGTCTGATGATAATAAAATCAGTTTTGGGAAAGGTCTTCCCAATTTTACCATGCGTAAAGCTTTTTCTGAGGTTGTTGCTGGAACAGCGACTGTAGATTCAGGATTTCCTCCTATCCAGCCGAGGAAAAAATCTTCATTGGGTTTAGATAAGATTATCAAGAAAACTAGGACAATGGATCAGCGTGTAGAACGGACTAGTGATAATGGAACTCGGAAATCTGAAGATGTTTTATCTGTTCAGGAAAACTTTAGTGAGACCAACAGCACAAATGGTGACCCCTATCTACAGATAGGTAGTAATGTAGTTCCTGTTAACCTGAATAGCGGTGAAAAGTTCAAAATGAACCCTTCTTTAAAACAGGTAGTAGTGTATGTTGGATTTGAGCATGAGTGCCCCCATGGCCACCGTTTTCTATTAAATCCAGAGCATCTTCATGAGCTTGGGTCCTCGTATCAATTGCCTGAAGAATCTCAGGAAAACTTAGACCATAGCCTGCCGGATAATTCGAAAATGAGTAGGAATGGTTTCCATGGTAAAGTTCACCGCAATTCAAATAGGATTGCTACTGCTACCGGTGCAAATAAGGAGAGACATGTAACTAAGTCAAAAGAGATAGTAACTATTGGTAATCTGCATATAGATGGGCTAATGCTGTCAGGACCAGGGAAGGAGCAGAATCACACATCTTTTAGTGCATCTCCAGTCCAGAATTTGCCCAAACGTCTTGAAGGGAGCTTTCAGTCTATTAGCCTTGATGATGGTGGATGTGCTTTCTCTATGTTGAATAGAAATTTACCAATCTATATGAACTGCCCACACTGCAAGCTCTCTAGGGATAAGCAAAATTCACTGAAGACGAAGTTTGCTGCCACAATATCACAGCTTCAAAGAGTTTTTGTGGTCACACCTCCCTTTCCAGTGATATTAGCTACTTGCCCAGTTATACAATTTGAGGCATCATCTCTACCTCCCTCAATCCCAGAACGTGAGCAGAAGTTGCAGTTCAACCTTGGATCCCAAGTGGTTTTACCTCCAGAGAGTTTCGTTACCCTTAGACTCCCATTTGTGTATGGTGTACAGCTGGAAGATGGAAGTTTACATTCTCTTGGTTGTTTCGAACATCAACCTGAAGTAACCGCTTGGATTACAAAAGGGACAACATTGCAGGTCATGTCGAAGAGGAACTGTGTTGGTCAAGATGGAGTTCTAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Smg8_Smg9

Protein Analysis

1221

Amino Acids

132.39

Weight (kDa)

8.93

Isoelectric Point (pI)

53.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Smg8_Smg9	PF10220	48 - 194	1.4e-11	Smg8_Smg9
Smg8_Smg9	PF10220	556 - 698	2.3e-37	Smg8_Smg9
Smg8_Smg9	PF10220	1075 - 1174	4.4e-07	Smg8_Smg9

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005910)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g30640 FvH4_3g30640 FvH4_3g30640 FvH4_3g30640
malus_domestica	MD03G1129000.v1.1
prunus_persica	Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1 Prupe.6G112700_v2.0.a1
pyrus_communis	pycom11g12120 pycom11g12130
rosa_chinensis	RchiOBHm_Chr5g0056861
rosa_laevigata	RLG00000035131
rosa_multiflora	Rmu_sc0001460.1_g000021
rosa_roxburghii	Rroxscaffold_1G00023440
rosa_rugosa	Rorug05G0302800
rosa_samantha	Rh5AG373000 Rh5BG384000 Rh5CG407200 Rh5DG397200
rosa_wichuraiana	Rw5G034970 Rw5G035030 Rw5G035080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	149
Acc36I	ACCTGC	2 cut(s)	2440, 3604
AccB1I	GGYRCC	2 cut(s)	5, 360
AccB7I	CCANNNNNTGG	1 cut(s)	1810
AccI	GTMKAC	2 cut(s)	1136, 1451
AclWI	GGATC	9 cut(s)	202, 215, 570, 1619, 1632, 2045, 2631, 3456, 3469
AcoI	YGGCCR	2 cut(s)	169, 2848
AcuI	CTGAAG	7 cut(s)	425, 1620, 2154, 2688, 2931, 3320, 3597
AfaI	GTAC	5 cut(s)	763, 1621, 2201, 2390, 3525
AfiI	CCNNNNNNNGG	4 cut(s)	370, 905, 1810, 2216
AflIII	ACRYGT	2 cut(s)	222, 3040
AgeI	ACCGGT	2 cut(s)	1321, 3020
AhdI	GACNNNNNGTC	2 cut(s)	2065, 2446
AhlI	ACTAGT	1 cut(s)	2642
AjnI	CCWGG	4 cut(s)	602, 751, 1650, 3109
AjuI	GAANNNNNNNTTGG	2 cut(s)	802, 834
Alw21I	GWGCWC	1 cut(s)	276
Alw26I	GTCTC	3 cut(s)	1930, 2696, 3031
AlwI	GGATC	9 cut(s)	202, 215, 570, 1619, 1632, 2045, 2631, 3456, 3469
AlwNI	CAGNNNCTG	4 cut(s)	282, 448, 1037, 2537
AoxI	GGCC	5 cut(s)	169, 605, 629, 887, 2848
AsiGI	ACCGGT	2 cut(s)	1321, 3020
Asp700I	GAANNNNTTC	1 cut(s)	2972
AspLEI	GCGC	1 cut(s)	448
AspS9I	GGNCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
AsuC2I	CCSGG	2 cut(s)	358, 394
AsuHPI	GGTGA	6 cut(s)	84, 1163, 1825, 2732, 2786, 2981
AsuII	TTCGAA	2 cut(s)	2954, 3564
AvaII	GGWCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
BaeGI	GKGCMC	3 cut(s)	8, 365, 2843
BalI	TGGCCA	1 cut(s)	2850
BamHI	GGATCC	3 cut(s)	207, 1624, 3461
BanI	GGYRCC	2 cut(s)	5, 360
BanII	GRGCYC	2 cut(s)	179, 276
BbsI	GAAGAC	2 cut(s)	2474, 3308
Bbv12I	GWGCWC	1 cut(s)	276
BccI	CCATC	8 cut(s)	37, 371, 1502, 1877, 3083, 3200, 3530, 3641
BceAI	ACGGC	2 cut(s)	353, 644
BcgI	CGANNNNNNTGC	4 cut(s)	170, 204, 512, 546
BciT130I	CCWGG	4 cut(s)	604, 753, 1652, 3111
BcnI	CCSGG	2 cut(s)	358, 394
BcoDI	GTCTC	3 cut(s)	1930, 2696, 3031
BcuI	ACTAGT	1 cut(s)	2642
BfaI	CTAG	9 cut(s)	47, 654, 867, 1631, 1781, 1953, 2613, 2643, 3279
BfmI	CTRYAG	4 cut(s)	1377, 2433, 2538, 2732
BfoI	RGCGCY	1 cut(s)	449
BfuAI	ACCTGC	2 cut(s)	2440, 3604
BglI	GCCNNNNNGGC	1 cut(s)	2847
BglII	AGATCT	1 cut(s)	2045
BmcAI	AGTACT	1 cut(s)	2201
Bme1390I	CCNGG	6 cut(s)	358, 394, 604, 753, 1652, 3111
Bme18I	GGWCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
BmeRI	GACNNNNNGTC	2 cut(s)	2065, 2446
BmgT120I	GGNCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
BmrFI	CCNGG	6 cut(s)	358, 394, 604, 753, 1652, 3111
BmrI	ACTGGG	3 cut(s)	761, 810, 3380
BmuI	ACTGGG	3 cut(s)	761, 810, 3380
BpiI	GAAGAC	2 cut(s)	2474, 3308
BpmI	CTGGAG	7 cut(s)	396, 512, 848, 1673, 2430, 3132, 3465
Bpu10I	CCTNAGC	1 cut(s)	275
Bpu14I	TTCGAA	2 cut(s)	2954, 3564
BpuEI	CTTGAG	3 cut(s)	281, 651, 2342
BpuMI	CCSGG	2 cut(s)	358, 394
BsaI	GGTCTC	1 cut(s)	2696
BsaJI	CCNNGG	7 cut(s)	369, 751, 2567, 2845, 2977, 3110, 3457
BsaWI	WCCGGW	3 cut(s)	204, 1321, 3020
BsaXI	ACNNNNNCTCC	2 cut(s)	1962, 1992
Bsc4I	CCNNNNNNNGG	4 cut(s)	370, 905, 1810, 2216
Bse118I	RCCGGY	2 cut(s)	1321, 3020
Bse3DI	GCAATG	2 cut(s)	703, 3608
BseBI	CCWGG	4 cut(s)	604, 753, 1652, 3111
BseDI	CCNNGG	7 cut(s)	369, 751, 2567, 2845, 2977, 3110, 3457
BseLI	CCNNNNNNNGG	4 cut(s)	370, 905, 1810, 2216
BseMI	GCAATG	2 cut(s)	703, 3608
BseMII	CTCAG	7 cut(s)	289, 942, 1044, 1224, 2261, 2505, 2933
BseRI	GAGGAG	3 cut(s)	109, 284, 845
BseSI	GKGCMC	3 cut(s)	8, 365, 2843
BsgI	GTGCAG	3 cut(s)	608, 2337, 2402
BshFI	GGCC	5 cut(s)	171, 607, 631, 889, 2850
BshNI	GGYRCC	2 cut(s)	5, 360
BshTI	ACCGGT	2 cut(s)	1321, 3020
BsiHKAI	GWGCWC	1 cut(s)	276
BsiSI	CCGG	7 cut(s)	172, 205, 358, 393, 1322, 2945, 3021
BslFI	GGGAC	4 cut(s)	293, 449, 1332, 3616
BslI	CCNNNNNNNGG	4 cut(s)	370, 905, 1810, 2216
BsmAI	GTCTC	3 cut(s)	1930, 2696, 3031
BsmFI	GGGAC	4 cut(s)	293, 449, 1332, 3616
BsmI	GAATGC	4 cut(s)	330, 976, 1249, 1865
BsnI	GGCC	5 cut(s)	171, 607, 631, 889, 2850
Bso31I	GGTCTC	1 cut(s)	2696
Bsp119I	TTCGAA	2 cut(s)	2954, 3564
Bsp1286I	GDGCHC	5 cut(s)	8, 179, 276, 365, 2843
Bsp1407I	TGTACA	2 cut(s)	2388, 3523
Bsp143I	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
Bsp19I	CCATGG	2 cut(s)	2845, 2977
BspANI	GGCC	5 cut(s)	171, 607, 631, 889, 2850
BspCNI	CTCAG	7 cut(s)	288, 943, 1043, 1223, 2260, 2506, 2932
BspHI	TCATGA	1 cut(s)	2881
BspMAI	CTGCAG	2 cut(s)	1381, 2437
BspMI	ACCTGC	2 cut(s)	2440, 3604
BspPI	GGATC	9 cut(s)	202, 215, 570, 1619, 1632, 2045, 2631, 3456, 3469
BspQI	GCTCTTC	2 cut(s)	414, 458
BspT104I	TTCGAA	2 cut(s)	2954, 3564
BspT107I	GGYRCC	2 cut(s)	5, 360
BspTNI	GGTCTC	1 cut(s)	2696
BsrDI	GCAATG	2 cut(s)	703, 3608
BsrFI	RCCGGY	2 cut(s)	1321, 3020
BsrGI	TGTACA	2 cut(s)	2388, 3523
BssAI	RCCGGY	2 cut(s)	1321, 3020
BssECI	CCNNGG	7 cut(s)	369, 751, 2567, 2845, 2977, 3110, 3457
BssMI	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
BssT1I	CCWWGG	3 cut(s)	2845, 2977, 3457
Bst2UI	CCWGG	4 cut(s)	604, 753, 1652, 3111
Bst6I	CTCTTC	6 cut(s)	111, 414, 458, 1224, 1713, 3620
BstAPI	GCANNNNNTGC	4 cut(s)	520, 1385, 1400, 3445
BstAUI	TGTACA	2 cut(s)	2388, 3523
BstBI	TTCGAA	2 cut(s)	2954, 3564
BstDSI	CCRYGG	3 cut(s)	369, 2845, 2977
BstEII	GGTNACC	1 cut(s)	2720
BstENI	CCTNNNNNAGG	1 cut(s)	2214
BstH2I	RGCGCY	1 cut(s)	449
BstHHI	GCGC	1 cut(s)	448
BstKTI	GATC	7 cut(s)	210, 578, 1627, 2048, 2053, 2626, 3464
BstMAI	GTCTC	3 cut(s)	1930, 2696, 3031
BstMBI	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
BstNI	CCWGG	4 cut(s)	604, 753, 1652, 3111
BstNSI	RCATGY	4 cut(s)	557, 1826, 2019, 3044
BstPI	GGTNACC	1 cut(s)	2720
BstSCI	CCNGG	6 cut(s)	356, 392, 602, 751, 1650, 3109
BstSFI	CTRYAG	4 cut(s)	1377, 2433, 2538, 2732
BstSLI	GKGCMC	3 cut(s)	8, 365, 2843
BstV2I	GAAGAC	2 cut(s)	2474, 3308
BstX2I	RGATCY	5 cut(s)	207, 575, 1624, 2045, 3461
BstYI	RGATCY	5 cut(s)	207, 575, 1624, 2045, 3461
BsuRI	GGCC	5 cut(s)	171, 607, 631, 889, 2850
BtgI	CCRYGG	3 cut(s)	369, 2845, 2977
BtsI	GCAGTG	3 cut(s)	1833, 2325, 3265
BveI	ACCTGC	2 cut(s)	2440, 3604
CaiI	CAGNNNCTG	4 cut(s)	282, 448, 1037, 2537
CciI	TCATGA	1 cut(s)	2881
CfoI	GCGC	1 cut(s)	448
Cfr10I	RCCGGY	2 cut(s)	1321, 3020
Cfr13I	GGNCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
Csp6I	GTAC	5 cut(s)	762, 1620, 2200, 2389, 3524
CspAI	ACCGGT	2 cut(s)	1321, 3020
CspCI	CAANNNNNGTGG	2 cut(s)	1159, 1194
CviQI	GTAC	5 cut(s)	762, 1620, 2200, 2389, 3524
DpnI	GATC	7 cut(s)	209, 577, 1626, 2047, 2052, 2625, 3463
DpnII	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
DraI	TTTAAA	1 cut(s)	2802
DrdI	GACNNNNNNGTC	1 cut(s)	149
DriI	GACNNNNNGTC	2 cut(s)	2065, 2446
DseDI	GACNNNNNNGTC	1 cut(s)	149
EaeI	YGGCCR	2 cut(s)	169, 2848
Eam1104I	CTCTTC	6 cut(s)	111, 414, 458, 1224, 1713, 3620
Eam1105I	GACNNNNNGTC	2 cut(s)	2065, 2446
EarI	CTCTTC	6 cut(s)	111, 414, 458, 1224, 1713, 3620
EciI	GGCGGA	1 cut(s)	41
Ecl136II	GAGCTC	1 cut(s)	274
Eco130I	CCWWGG	3 cut(s)	2845, 2977, 3457
Eco24I	GRGCYC	2 cut(s)	179, 276
Eco31I	GGTCTC	1 cut(s)	2696
Eco32I	GATATC	1 cut(s)	1261
Eco47I	GGWCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
Eco53kI	GAGCTC	1 cut(s)	274
Eco57I	CTGAAG	7 cut(s)	425, 1620, 2154, 2688, 2931, 3320, 3597
Eco91I	GGTNACC	1 cut(s)	2720
EcoICRI	GAGCTC	1 cut(s)	274
EcoNI	CCTNNNNNAGG	1 cut(s)	2214
EcoO109I	RGGNCCY	3 cut(s)	43, 436, 2892
EcoO65I	GGTNACC	1 cut(s)	2720
EcoRII	CCWGG	4 cut(s)	602, 751, 1650, 3109
EcoRV	GATATC	1 cut(s)	1261
EcoT14I	CCWWGG	3 cut(s)	2845, 2977, 3457
EcoT22I	ATGCAT	1 cut(s)	1108
EcoT38I	GRGCYC	2 cut(s)	179, 276
ErhI	CCWWGG	3 cut(s)	2845, 2977, 3457
FalI	AAGNNNNNCTT	2 cut(s)	2208, 2240
FaqI	GGGAC	4 cut(s)	293, 449, 1332, 3616
FauI	CCCGC	7 cut(s)	33, 142, 373, 941, 1045, 1058, 2225
FblI	GTMKAC	2 cut(s)	1136, 1451
FriOI	GRGCYC	2 cut(s)	179, 276
FspBI	CTAG	9 cut(s)	47, 654, 867, 1631, 1781, 1953, 2613, 2643, 3279
GlaI	GCGC	1 cut(s)	447
GsuI	CTGGAG	7 cut(s)	396, 512, 848, 1673, 2430, 3132, 3465
HaeII	RGCGCY	1 cut(s)	449
HaeIII	GGCC	5 cut(s)	171, 607, 631, 889, 2850
HapII	CCGG	7 cut(s)	172, 205, 358, 393, 1322, 2945, 3021
HhaI	GCGC	1 cut(s)	448
Hin6I	GCGC	1 cut(s)	446
HinP1I	GCGC	1 cut(s)	446
HincII	GTYRAC	2 cut(s)	1496, 2761
HindII	GTYRAC	2 cut(s)	1496, 2761
HindIII	AAGCTT	3 cut(s)	892, 1797, 2505
HpaI	GTTAAC	1 cut(s)	2761
HpaII	CCGG	7 cut(s)	172, 205, 358, 393, 1322, 2945, 3021
HphI	GGTGA	6 cut(s)	84, 1163, 1825, 2732, 2786, 2981
Hpy99I	CGWCG	3 cut(s)	146, 158, 185
HpyCH4IV	ACGT	3 cut(s)	222, 3169, 3433
HpySE526I	ACGT	3 cut(s)	222, 3169, 3433
HspAI	GCGC	1 cut(s)	446
KspAI	GTTAAC	1 cut(s)	2761
Kzo9I	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
LguI	GCTCTTC	2 cut(s)	414, 458
LmnI	GCTCC	5 cut(s)	174, 271, 568, 3118, 3180
MaeI	CTAG	9 cut(s)	47, 654, 867, 1631, 1781, 1953, 2613, 2643, 3279
MaeII	ACGT	3 cut(s)	222, 3169, 3433
MalI	GATC	7 cut(s)	209, 577, 1626, 2047, 2052, 2625, 3463
MboI	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
MfeI	CAATTG	1 cut(s)	2903
MflI	RGATCY	5 cut(s)	207, 575, 1624, 2045, 3461
MhlI	GDGCHC	5 cut(s)	8, 179, 276, 365, 2843
MlsI	TGGCCA	1 cut(s)	2850
MluNI	TGGCCA	1 cut(s)	2850
MlyI	GAGTC	7 cut(s)	179, 209, 401, 685, 842, 1699, 3498
MmeI	TCCRAC	5 cut(s)	165, 243, 1830, 2376, 2803
Mox20I	TGGCCA	1 cut(s)	2850
Mph1103I	ATGCAT	1 cut(s)	1108
MroXI	GAANNNNTTC	1 cut(s)	2972
MscI	TGGCCA	1 cut(s)	2850
MseI	TTAA	8 cut(s)	855, 903, 1007, 1011, 2402, 2760, 2801, 2864
MslI	CAYNNNNRTG	3 cut(s)	1992, 2880, 3087
Msp20I	TGGCCA	1 cut(s)	2850
MspA1I	CMGCKG	7 cut(s)	644, 1382, 1391, 1400, 1430, 1715, 3529
MspI	CCGG	7 cut(s)	172, 205, 358, 393, 1322, 2945, 3021
MspR9I	CCNGG	6 cut(s)	358, 394, 604, 753, 1652, 3111
MunI	CAATTG	1 cut(s)	2903
Mva1269I	GAATGC	4 cut(s)	330, 976, 1249, 1865
MvaI	CCWGG	4 cut(s)	604, 753, 1652, 3111
NciI	CCSGG	2 cut(s)	358, 394
NcoI	CCATGG	2 cut(s)	2845, 2977
NdeII	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
NmeAIII	GCCGAG	1 cut(s)	2592
NmuCI	GTSAC	4 cut(s)	769, 2102, 2720, 3349
NsiI	ATGCAT	1 cut(s)	1108
NspI	RCATGY	4 cut(s)	557, 1826, 2019, 3044
NspV	TTCGAA	2 cut(s)	2954, 3564
PacI	TTAATTAA	1 cut(s)	1011
PaeI	GCATGC	1 cut(s)	557
PagI	TCATGA	1 cut(s)	2881
PciI	ACATGT	1 cut(s)	3040
PciSI	GCTCTTC	2 cut(s)	414, 458
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 219
PctI	GAATGC	4 cut(s)	330, 976, 1249, 1865
PdmI	GAANNNNTTC	1 cut(s)	2972
PflFI	GACNNNGTC	1 cut(s)	1447
PflMI	CCANNNNNTGG	1 cut(s)	1810
PinAI	ACCGGT	2 cut(s)	1321, 3020
PleI	GAGTC	7 cut(s)	179, 209, 401, 685, 841, 1699, 3498
PpsI	GAGTC	7 cut(s)	179, 209, 401, 685, 841, 1699, 3498
PpuMI	RGGWCCY	3 cut(s)	43, 436, 2892
PscI	ACATGT	1 cut(s)	3040
Psp124BI	GAGCTC	1 cut(s)	276
Psp5II	RGGWCCY	3 cut(s)	43, 436, 2892
Psp6I	CCWGG	4 cut(s)	602, 751, 1650, 3109
PspEI	GGTNACC	1 cut(s)	2720
PspGI	CCWGG	4 cut(s)	602, 751, 1650, 3109
PspPI	GGNCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
PspPPI	RGGWCCY	3 cut(s)	43, 436, 2892
PstI	CTGCAG	2 cut(s)	1381, 2437
PstNI	CAGNNNCTG	4 cut(s)	282, 448, 1037, 2537
PsuI	RGATCY	5 cut(s)	207, 575, 1624, 2045, 3461
PsyI	GACNNNGTC	1 cut(s)	1447
PvuII	CAGCTG	4 cut(s)	1382, 1400, 1715, 3529
RsaI	GTAC	5 cut(s)	763, 1621, 2201, 2390, 3525
RsaNI	GTAC	5 cut(s)	762, 1620, 2200, 2389, 3524
RseI	CAYNNNNRTG	3 cut(s)	1992, 2880, 3087
SacI	GAGCTC	1 cut(s)	276
SapI	GCTCTTC	2 cut(s)	414, 458
SaqAI	TTAA	8 cut(s)	855, 903, 1007, 1011, 2402, 2760, 2801, 2864
Sau3AI	GATC	7 cut(s)	207, 575, 1624, 2045, 2050, 2623, 3461
Sau96I	GGNCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
ScaI	AGTACT	1 cut(s)	2201
SchI	GAGTC	7 cut(s)	179, 209, 401, 685, 842, 1699, 3498
ScrFI	CCNGG	6 cut(s)	358, 394, 604, 753, 1652, 3111
SduI	GDGCHC	5 cut(s)	8, 179, 276, 365, 2843
SfcI	CTRYAG	4 cut(s)	1377, 2433, 2538, 2732
SfuI	TTCGAA	2 cut(s)	2954, 3564
SinI	GGWCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
SmiMI	CAYNNNNRTG	3 cut(s)	1992, 2880, 3087
SmlI	CTYRAG	3 cut(s)	260, 666, 2357
SmoI	CTYRAG	3 cut(s)	260, 666, 2357
SpeI	ACTAGT	1 cut(s)	2642
SphI	GCATGC	1 cut(s)	557
SspMI	CTAG	9 cut(s)	47, 654, 867, 1631, 1781, 1953, 2613, 2643, 3279
SstI	GAGCTC	1 cut(s)	276
StyD4I	CCNGG	6 cut(s)	356, 392, 602, 751, 1650, 3109
StyI	CCWWGG	3 cut(s)	2845, 2977, 3457
TaiI	ACGT	3 cut(s)	225, 3172, 3436
TaqI	TCGA	6 cut(s)	213, 243, 532, 2954, 3564, 3623
TatI	WGTACW	3 cut(s)	2199, 2388, 3523
TauI	GCSGC	1 cut(s)	1391
Tru1I	TTAA	8 cut(s)	855, 903, 1007, 1011, 2402, 2760, 2801, 2864
Tru9I	TTAA	8 cut(s)	855, 903, 1007, 1011, 2402, 2760, 2801, 2864
TseFI	GTSAC	4 cut(s)	769, 2102, 2720, 3349
Tsp45I	GTSAC	4 cut(s)	769, 2102, 2720, 3349
TspGWI	ACGGA	4 cut(s)	555, 1637, 2050, 2653
Tth111I	GACNNNGTC	1 cut(s)	1447
Van91I	CCANNNNNTGG	1 cut(s)	1810
VpaK11BI	GGWCC	6 cut(s)	43, 436, 1319, 2179, 2892, 3107
XagI	CCTNNNNNAGG	1 cut(s)	2214
XbaI	TCTAGA	2 cut(s)	1630, 1780
XceI	RCATGY	4 cut(s)	557, 1826, 2019, 3044
XcmI	CCANNNNNNNNNTGG	3 cut(s)	556, 823, 1177
XmiI	GTMKAC	2 cut(s)	1136, 1451
XmnI	GAANNNNTTC	1 cut(s)	2972
XspI	CTAG	9 cut(s)	47, 654, 867, 1631, 1781, 1953, 2613, 2643, 3279
ZrmI	AGTACT	1 cut(s)	2201
Zsp2I	ATGCAT	1 cut(s)	1108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.