Rorug05G0302800

Smg8_Smg9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005910
Physical Location & Seq
Reverse (-)
35292003 .. 35294671
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0302800.1

Sequence Viewer

Length: 1557 bp
ATGGAACTACAATGCCCTTCCTTCCAAGTCATGTTTACTTTCTTGGTCCTTGTGATTGTGGTACTGAAAATAGGGAGGAGAGGCCAAAACAACAACTCCTCTGCTTCAAATCTACCACCAGGGCCATGGAAGCTACCAATTATTGGAAACATACACCAGCTAGTTGGCACTCTACCCCATCGTGGACTCAGAGACTTGGCCAAGCAACATGGACCGCTTATGCATCTGAGGCTCGGAGAAGTTTCCACCGTAGTTGTTTCATCAGCAGAGTTTGCCAAAGAGGTGATGAAAACCCATGATATTATCTTTGCTTCAAGGCCTCAGGTCCTAGCTTCAAGAATCTTATCATATGGTTCTACAAATATTGCTTTTGCTCCATATGGTAATTACTGGAGAAGATTACGAAAAATTTGCATGCAGGAGCTCTTAAGTGCAAAGCGGGTTCAATCTTACCGGCCTATTAGGGAAAAAGAGGTACTGAATCTCATCGGATGGATTGGTGCAAGAGCAGAATCACCTATTAATCTTACTCAGAAAGTTTTATCTTCCACATATAGCATCACTTCACAAGCAGCCTTTGGCAATCAATGCAGCGATCAAGAAAATTTCTTATATGTGGTGAAGCAAGCTATCAAAGAAGCATCAGGCTTCAATGTGGCAGATGCTTTTCCATCTTTCAAATTACTTCATATCATAAGTGGAGTGCAGCCTAAACTTGAGAGGTTGCAGAAACAAGCTGACAGAATAATGGAAAACATCATCAAAGAACATATACAAGCTACCACAACAACGAAAAGTGATGAGGCTGAAGTAGAGGAAGATCTGGTAGATGTTCTCCTAAAATTTCATGACCAAGGTGGTGGCCATGAGTTTTCTTTGACTATTGACAACATCAAAGCAGTGATCTTGGATATTTTTACTGCTGGGAGTGAGACATCAGCTACAACTGTAGATTGGACAATGTCAGAAATGATAAAACATCCGAGAATCATGAAAAGGGCACAGGATGAGGTGAGGGAAGTGTTCAACAGAACACATCAGGTAAACGAAACAGGCATTAGAGAGATGAAATATTTAAAGTTAGTTATTCAAGAGACACTGAGGTTACACCCTGTCGCTCCGCTGTTACTTCCAAGAGAATGTGGAGAGAGGTGTGAAATTGATGGATATGAAATACCTGTCAAAACCAAGGTAATTGTGAATGCTTGGGCAATCGGAAGAGATCCCAATTACTGGACTGAGCCCGAGACCTTTTATCCAGAGAGATTCCTTGATAGCTCTATTGACTACCAAGGAACAAACTTTGATTATATCCCATTTGGTGCTGGAAGAAGAATATGCCCTGGCATATTATATGGTCTGGCTAATGTTGAGCTCCCACTAGCTTTGTTGTTATATCATTTTGATTGGAAGCTCCCCAATGGAACAAAGAATGAAGACTTGGACATGACTGAGGACTTTGGCGCTGTAGTCCACAGAAAAGAAGATCTGCATTTAATTCCCACTCCATATCATCCTCCTCCAACTGATGCAAATTCAAAAATAGAAATAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Smg8_Smg9

Protein Analysis

518

Amino Acids

58.63

Weight (kDa)

6.48

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 495 4.4e-102 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 143, 1233
AciI CCGC 3 cut(s) 215, 439, 1121
AclWI GGATC 1 cut(s) 1217
AcoI YGGCCR 2 cut(s) 198, 862
AcsI RAATTY 4 cut(s) 408, 604, 842, 1534
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 2 cut(s) 63, 477
AfiI CCNNNNNNNGG 3 cut(s) 143, 182, 1233
AflII CTTAAG 1 cut(s) 427
AgsI TTSAA 9 cut(s) 108, 315, 336, 446, 652, 679, 1027, 1091, 1539
AjnI CCWGG 2 cut(s) 118, 1342
AjuI GAANNNNNNNTTGG 2 cut(s) 1424, 1456
Alw21I GWGCWC 2 cut(s) 426, 1377
Alw26I GTCTC 4 cut(s) 186, 926, 1088, 1241
AlwI GGATC 1 cut(s) 1217
Ama87I CYCGRG 1 cut(s) 1244
AoxI GGCC 6 cut(s) 82, 122, 198, 317, 455, 862
ApeKI GCWGC 3 cut(s) 572, 591, 706
ApoI RAATTY 4 cut(s) 408, 604, 842, 1534
AseI ATTAAT 1 cut(s) 522
AspLEI GCGC 1 cut(s) 1466
AspS9I GGNCC 4 cut(s) 46, 122, 212, 325
AsuHPI GGTGA 4 cut(s) 295, 507, 631, 1024
AvaI CYCGRG 1 cut(s) 1244
AvaII GGWCC 3 cut(s) 46, 212, 325
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 1003
BalI TGGCCA 2 cut(s) 200, 864
BanII GRGCYC 3 cut(s) 426, 1245, 1377
BbsI GAAGAC 1 cut(s) 1443
Bbv12I GWGCWC 2 cut(s) 426, 1377
BbvI GCAGC 3 cut(s) 584, 603, 718
BccI CCATC 4 cut(s) 186, 486, 679, 1157
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 2 cut(s) 120, 1344
BcoDI GTCTC 4 cut(s) 186, 926, 1088, 1241
BfaI CTAG 3 cut(s) 161, 329, 1382
BfmI CTRYAG 2 cut(s) 948, 1467
BfoI RGCGCY 1 cut(s) 1467
BfrI CTTAAG 1 cut(s) 427
BglII AGATCT 2 cut(s) 820, 1486
BisI GCNGC 3 cut(s) 573, 592, 707
BlsI GCNGC 3 cut(s) 574, 593, 708
Bme1390I CCNGG 2 cut(s) 120, 1344
Bme18I GGWCC 3 cut(s) 46, 212, 325
BmeT110I CYCGRG 1 cut(s) 1244
BmgT120I GGNCC 4 cut(s) 46, 122, 212, 325
BmrFI CCNGG 2 cut(s) 120, 1344
BmsI GCATC 5 cut(s) 232, 567, 650, 652, 1519
BpiI GAAGAC 1 cut(s) 1443
BpmI CTGGAG 1 cut(s) 412
BpuEI CTTGAG 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 1241
BsaJI CCNNGG 6 cut(s) 119, 125, 853, 1188, 1291, 1342
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 182, 1233
Bse118I RCCGGY 1 cut(s) 453
Bse1I ACTGG 2 cut(s) 395, 1238
Bse21I CCTNAGG 1 cut(s) 321
BseBI CCWGG 2 cut(s) 120, 1344
BseDI CCNNGG 6 cut(s) 119, 125, 853, 1188, 1291, 1342
BseGI GGATG 4 cut(s) 497, 979, 1012, 1513
BseLI CCNNNNNNNGG 3 cut(s) 143, 182, 1233
BseMII CTCAG 7 cut(s) 202, 218, 335, 545, 1091, 1230, 1443
BseNI ACTGG 2 cut(s) 395, 1238
BseRI GAGGAG 3 cut(s) 88, 91, 1509
BseSI GKGCMC 1 cut(s) 1003
BseXI GCAGC 3 cut(s) 584, 603, 718
BseYI CCCAGC 1 cut(s) 923
BsgI GTGCAG 1 cut(s) 725
BshFI GGCC 6 cut(s) 84, 124, 200, 319, 457, 864
BsiHKAI GWGCWC 2 cut(s) 426, 1377
BsiHKCI CYCGRG 1 cut(s) 1244
BsiSI CCGG 1 cut(s) 454
BslI CCNNNNNNNGG 3 cut(s) 143, 182, 1233
BsmAI GTCTC 4 cut(s) 186, 926, 1088, 1241
BsmI GAATGC 1 cut(s) 1207
BsnI GGCC 6 cut(s) 84, 124, 200, 319, 457, 864
Bso31I GGTCTC 1 cut(s) 1241
BsoBI CYCGRG 1 cut(s) 1244
Bsp1286I GDGCHC 4 cut(s) 426, 1003, 1245, 1377
Bsp143I GATC 5 cut(s) 595, 820, 903, 1222, 1486
Bsp19I CCATGG 1 cut(s) 125
BspACI CCGC 3 cut(s) 215, 439, 1121
BspANI GGCC 6 cut(s) 84, 124, 200, 319, 457, 864
BspCNI CTCAG 7 cut(s) 201, 219, 334, 544, 1092, 1231, 1444
BspHI TCATGA 2 cut(s) 847, 990
BspPI GGATC 1 cut(s) 1217
BspTI CTTAAG 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 1241
BsrFI RCCGGY 1 cut(s) 453
BsrI ACTGG 2 cut(s) 395, 1238
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 6 cut(s) 119, 125, 853, 1188, 1291, 1342
BssMI GATC 5 cut(s) 595, 820, 903, 1222, 1486
BssT1I CCWWGG 4 cut(s) 125, 853, 1188, 1291
Bst2UI CCWGG 2 cut(s) 120, 1344
Bst4CI ACNGT 2 cut(s) 250, 949
Bst6I CTCTTC 1 cut(s) 1213
BstAFI CTTAAG 1 cut(s) 427
BstAPI GCANNNNNTGC 2 cut(s) 272, 588
BstC8I GCNNGC 2 cut(s) 416, 627
BstDEI CTNAG 7 cut(s) 188, 227, 321, 531, 1100, 1239, 1452
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 4 cut(s) 497, 979, 1012, 1513
BstH2I RGCGCY 1 cut(s) 1467
BstHHI GCGC 1 cut(s) 1466
BstKTI GATC 5 cut(s) 598, 823, 906, 1225, 1489
BstMAI GTCTC 4 cut(s) 186, 926, 1088, 1241
BstMBI GATC 5 cut(s) 595, 820, 903, 1222, 1486
BstMWI GCNNNNNNNGC 4 cut(s) 130, 229, 272, 588
BstNI CCWGG 2 cut(s) 120, 1344
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 2 cut(s) 118, 1342
BstSFI CTRYAG 2 cut(s) 948, 1467
BstSLI GKGCMC 1 cut(s) 1003
BstV1I GCAGC 3 cut(s) 584, 603, 718
BstV2I GAAGAC 1 cut(s) 1443
BstX2I RGATCY 3 cut(s) 820, 1222, 1486
BstXI CCANNNNNNTGG 3 cut(s) 126, 164, 860
BstYI RGATCY 3 cut(s) 820, 1222, 1486
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 6 cut(s) 84, 124, 200, 319, 457, 864
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 4 cut(s) 497, 979, 1012, 1513
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 2 cut(s) 906, 1097
Cac8I GCNNGC 2 cut(s) 416, 627
CciI TCATGA 2 cut(s) 847, 990
CfoI GCGC 1 cut(s) 1466
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 4 cut(s) 46, 122, 212, 325
Csp6I GTAC 2 cut(s) 62, 476
CspCI CAANNNNNGTGG 4 cut(s) 235, 270, 1368, 1403
CviAII CATG 9 cut(s) 31, 126, 209, 296, 415, 848, 866, 991, 1447
CviQI GTAC 2 cut(s) 62, 476
DdeI CTNAG 7 cut(s) 188, 227, 321, 531, 1100, 1239, 1452
DpnI GATC 5 cut(s) 597, 822, 905, 1224, 1488
DpnII GATC 5 cut(s) 595, 820, 903, 1222, 1486
DraI TTTAAA 1 cut(s) 1077
EaeI YGGCCR 2 cut(s) 198, 862
Eam1104I CTCTTC 1 cut(s) 1213
EarI CTCTTC 1 cut(s) 1213
Ecl136II GAGCTC 2 cut(s) 424, 1375
Eco130I CCWWGG 4 cut(s) 125, 853, 1188, 1291
Eco147I AGGCCT 1 cut(s) 319
Eco24I GRGCYC 3 cut(s) 426, 1245, 1377
Eco31I GGTCTC 1 cut(s) 1241
Eco47I GGWCC 3 cut(s) 46, 212, 325
Eco53kI GAGCTC 2 cut(s) 424, 1375
Eco57I CTGAAG 1 cut(s) 828
Eco81I CCTNAGG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 1244
EcoICRI GAGCTC 2 cut(s) 424, 1375
EcoO109I RGGNCCY 1 cut(s) 325
EcoRII CCWGG 2 cut(s) 118, 1342
EcoT14I CCWWGG 4 cut(s) 125, 853, 1188, 1291
EcoT22I ATGCAT 1 cut(s) 225
EcoT38I GRGCYC 3 cut(s) 426, 1245, 1377
ErhI CCWWGG 4 cut(s) 125, 853, 1188, 1291
FaeI CATG 9 cut(s) 34, 129, 212, 299, 418, 851, 869, 994, 1450
FatI CATG 9 cut(s) 30, 125, 208, 295, 414, 847, 865, 990, 1446
FauI CCCGC 1 cut(s) 432
FauNDI CATATG 2 cut(s) 349, 379
Fnu4HI GCNGC 3 cut(s) 573, 592, 707
FokI GGATG 4 cut(s) 504, 966, 1019, 1500
FriOI GRGCYC 3 cut(s) 426, 1245, 1377
Fsp4HI GCNGC 3 cut(s) 573, 592, 707
FspBI CTAG 3 cut(s) 161, 329, 1382
GlaI GCGC 1 cut(s) 1465
GluI GCNGC 3 cut(s) 573, 592, 707
GsaI CCCAGC 1 cut(s) 927
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 1467
HaeIII GGCC 6 cut(s) 84, 124, 200, 319, 457, 864
HapII CCGG 1 cut(s) 454
HhaI GCGC 1 cut(s) 1466
Hin1II CATG 9 cut(s) 34, 129, 212, 299, 418, 851, 869, 994, 1450
Hin6I GCGC 1 cut(s) 1464
HinP1I GCGC 1 cut(s) 1464
HinfI GANTC 6 cut(s) 186, 339, 481, 512, 987, 1266
HpaII CCGG 1 cut(s) 454
HphI GGTGA 4 cut(s) 295, 507, 631, 1024
Hpy166II GTNNAC 4 cut(s) 36, 185, 1045, 1474
Hpy188I TCNGA 8 cut(s) 191, 228, 236, 491, 534, 967, 984, 1217
Hpy188III TCNNGA 6 cut(s) 336, 599, 848, 991, 1091, 1259
Hpy8I GTNNAC 4 cut(s) 36, 185, 1045, 1474
HpyAV CCTTC 2 cut(s) 27, 31
HpyCH4III ACNGT 2 cut(s) 250, 949
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 229, 272, 588
HpyF3I CTNAG 7 cut(s) 188, 227, 321, 531, 1100, 1239, 1452
Hsp92II CATG 9 cut(s) 34, 129, 212, 299, 418, 851, 869, 994, 1450
HspAI GCGC 1 cut(s) 1464
Kzo9I GATC 5 cut(s) 595, 820, 903, 1222, 1486
LmnI GCTCC 5 cut(s) 379, 421, 1123, 1380, 1419
Lsp1109I GCAGC 3 cut(s) 584, 603, 718
LweI GCATC 5 cut(s) 232, 567, 650, 652, 1519
MaeI CTAG 3 cut(s) 161, 329, 1382
MaeIII GTNAC 2 cut(s) 1104, 1125
MalI GATC 5 cut(s) 597, 822, 905, 1224, 1488
MboI GATC 5 cut(s) 595, 820, 903, 1222, 1486
MboII GAAGA 8 cut(s) 408, 537, 830, 1230, 1341, 1344, 1448, 1496
MflI RGATCY 3 cut(s) 820, 1222, 1486
MhlI GDGCHC 4 cut(s) 426, 1003, 1245, 1377
MlsI TGGCCA 2 cut(s) 200, 864
MluNI TGGCCA 2 cut(s) 200, 864
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 1547
Mox20I TGGCCA 2 cut(s) 200, 864
Mph1103I ATGCAT 1 cut(s) 225
MscI TGGCCA 2 cut(s) 200, 864
MseI TTAA 5 cut(s) 428, 522, 1076, 1496, 1555
Msp20I TGGCCA 2 cut(s) 200, 864
MspA1I CMGCKG 1 cut(s) 1123
MspCI CTTAAG 1 cut(s) 427
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 2 cut(s) 120, 1344
Mva1269I GAATGC 1 cut(s) 1207
MvaI CCWGG 2 cut(s) 120, 1344
MwoI GCNNNNNNNGC 4 cut(s) 130, 229, 272, 588
NcoI CCATGG 1 cut(s) 125
NdeI CATATG 2 cut(s) 349, 379
NdeII GATC 5 cut(s) 595, 820, 903, 1222, 1486
NlaIII CATG 9 cut(s) 34, 129, 212, 299, 418, 851, 869, 994, 1450
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 1 cut(s) 418
PaeI GCATGC 1 cut(s) 418
PagI TCATGA 2 cut(s) 847, 990
PceI AGGCCT 1 cut(s) 319
PctI GAATGC 1 cut(s) 1207
PfeI GAWTC 5 cut(s) 339, 481, 512, 987, 1266
PflFI GACNNNGTC 1 cut(s) 961
PflMI CCANNNNNTGG 2 cut(s) 143, 1233
PkrI GCNGC 3 cut(s) 574, 593, 708
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 325
PshBI ATTAAT 1 cut(s) 522
Psp124BI GAGCTC 2 cut(s) 426, 1377
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 118, 1342
PspFI CCCAGC 1 cut(s) 923
PspGI CCWGG 2 cut(s) 118, 1342
PspPI GGNCC 4 cut(s) 46, 122, 212, 325
PspPPI RGGWCCY 1 cut(s) 325
PsuI RGATCY 3 cut(s) 820, 1222, 1486
PsyI GACNNNGTC 1 cut(s) 961
RsaI GTAC 2 cut(s) 63, 477
RsaNI GTAC 2 cut(s) 62, 476
SacI GAGCTC 2 cut(s) 426, 1377
SaqAI TTAA 5 cut(s) 428, 522, 1076, 1496, 1555
SatI GCNGC 3 cut(s) 573, 592, 707
Sau3AI GATC 5 cut(s) 595, 820, 903, 1222, 1486
Sau96I GGNCC 4 cut(s) 46, 122, 212, 325
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 2 cut(s) 120, 1344
SduI GDGCHC 4 cut(s) 426, 1003, 1245, 1377
SfaNI GCATC 5 cut(s) 232, 567, 650, 652, 1519
SfcI CTRYAG 2 cut(s) 948, 1467
SinI GGWCC 3 cut(s) 46, 212, 325
SmlI CTYRAG 2 cut(s) 427, 716
SmoI CTYRAG 2 cut(s) 427, 716
SphI GCATGC 1 cut(s) 418
SseBI AGGCCT 1 cut(s) 319
SsiI CCGC 3 cut(s) 215, 439, 1121
SspI AATATT 2 cut(s) 364, 1073
SspMI CTAG 3 cut(s) 161, 329, 1382
SstI GAGCTC 2 cut(s) 426, 1377
StuI AGGCCT 1 cut(s) 319
StyD4I CCNGG 2 cut(s) 118, 1342
StyI CCWWGG 4 cut(s) 125, 853, 1188, 1291
TaaI ACNGT 2 cut(s) 250, 949
TfiI GAWTC 5 cut(s) 339, 481, 512, 987, 1266
Tru1I TTAA 5 cut(s) 428, 522, 1076, 1496, 1555
Tru9I TTAA 5 cut(s) 428, 522, 1076, 1496, 1555
TscAI CASTG 2 cut(s) 906, 1104
TseI GCWGC 3 cut(s) 572, 591, 706
TspDTI ATGAA 8 cut(s) 249, 302, 677, 836, 1007, 1082, 1185, 1449
TspRI CASTG 2 cut(s) 906, 1104
Tth111I GACNNNGTC 1 cut(s) 961
Van91I CCANNNNNTGG 2 cut(s) 143, 1233
Vha464I CTTAAG 1 cut(s) 427
VpaK11BI GGWCC 3 cut(s) 46, 212, 325
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 4 cut(s) 408, 604, 842, 1534
XceI RCATGY 1 cut(s) 418
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
XspI CTAG 3 cut(s) 161, 329, 1382
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.