MD03G1130200.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016302
Physical Location & Seq
Forward (+)
12809656 .. 12813252
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1130200.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGTGGGTTGGCAGTTGCGTGTACAGTCCCTAATCCGTCAAGTTGGGAAAAGAATGGAGAATTATAGTACTTCTTCTTTTTCTCCTTGTCATTTTCCGAAACTCCAGACACCACCTTCTCAAACCATGTCAATGCCCCTTTATCACTACTGTCAACAGTTGGGAATTTCCAGTTCAAGGAACTTGCTTGCAGATTTGTCTGCTGGAGTTGCTCCTCTATCAGCATCAGATGGTGAAAACGGTGAAGAACGGGACCGGAAATCACCTTCTGGACCTTCACAAGTTCAATTTGTCTTAAAGGGAATTAATCAGAGCCCTAATAAGGTCAACTTGGTTGCTAAACTGGTTCGTGGTATGCGTGTTGAAGATGCATTATTGCAACTGCAAGTGACAGACAAGCAAGCAGCAAAAACTGTGCATCAGGCTCTTCACTCAGCCCGGGCAGATGCAACTTATAATCATGGGTTGGATCCAGAACGTCTTCTTGTTGCTGGGGCCTTGGTTGGAAAGGGATTCGTGGGATTCCGCAAGAACAGACTTTACTACCATGGTAAAAGCAATGATGGAACTAAAGTGAGACCCAATTACCAGCTAACGGTGATGCTAAGGGAGATAGCTCCCGAAGAGGAGGAGGAGATAACCAAACAGAGAGTTAACAATTTTCTCTATCTCAAGAAACGCCTCAGGCTCACGAAGCAGGAAAATAAATTTGTTTCTCACTACGTTACCAGCAATCACAAAGGCAAAGCCGGTACCAGTCAACCAAGCGGTCTGGCTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

261

Amino Acids

28.96

Weight (kDa)

9.88

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 99 - 202 2.7e-19 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00560
malus_domestica MD03G1130200.v1.1 MD06G1011100.v1.1
prunus_persica Prupe.2G035300_v2.0.a1 Prupe.7G027600_v2.0.a1
pyrus_communis pycom06g00910
rosa_chinensis RchiOBHm_Chr4g0386011
rosa_laevigata RLG00000010181
rosa_multiflora Rmu_sc0000472.1_g000020
rosa_rugosa Rorug03G0306200
rosa_samantha Rh4AG010000 Rh4BG007000 Rh4CG010700 Rh4DG008100
rosa_wichuraiana Rw4G000140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 752
AccB1I GGYRCC 1 cut(s) 752
AciI CCGC 2 cut(s) 526, 768
AclWI GGATC 2 cut(s) 464, 477
AcsI RAATTY 2 cut(s) 165, 707
AfaI GTAC 3 cut(s) 24, 70, 754
AfiI CCNNNNNNNGG 3 cut(s) 177, 322, 595
AgsI TTSAA 3 cut(s) 177, 287, 365
AluBI AGCT 2 cut(s) 592, 617
AluI AGCT 2 cut(s) 592, 617
Alw26I GTCTC 1 cut(s) 571
AlwI GGATC 2 cut(s) 464, 477
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 2 cut(s) 165, 707
AseI ATTAAT 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 480
Asp718I GGTACC 1 cut(s) 752
AspS9I GGNCC 3 cut(s) 253, 272, 495
AsuC2I CCSGG 2 cut(s) 439, 440
AsuHPI GGTGA 4 cut(s) 245, 254, 255, 610
AvaI CYCGRG 1 cut(s) 438
AvaII GGWCC 2 cut(s) 253, 272
AxyI CCTNAGG 1 cut(s) 683
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 752
BanII GRGCYC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 416
BccI CCATC 2 cut(s) 224, 557
BcnI CCSGG 2 cut(s) 439, 440
BcoDI GTCTC 1 cut(s) 571
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
BmcAI AGTACT 1 cut(s) 70
Bme1390I CCNGG 2 cut(s) 439, 440
Bme18I GGWCC 2 cut(s) 253, 272
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 3 cut(s) 253, 272, 495
BmiI GGNNCC 4 cut(s) 254, 471, 496, 754
BmrFI CCNGG 2 cut(s) 439, 440
BmsI GCATC 5 cut(s) 233, 358, 427, 436, 591
BpiI GAAGAC 1 cut(s) 473
BpmI CTGGAG 2 cut(s) 89, 225
Bpu10I CCTNAGC 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 2 cut(s) 439, 440
BsaI GGTCTC 1 cut(s) 571
BsaJI CCNNGG 3 cut(s) 438, 498, 547
BsaWI WCCGGW 1 cut(s) 255
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 322, 595
Bse118I RCCGGY 1 cut(s) 749
Bse1I ACTGG 3 cut(s) 171, 348, 756
Bse21I CCTNAGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 565
BseDI CCNNGG 3 cut(s) 438, 498, 547
BseLI CCNNNNNNNGG 3 cut(s) 177, 322, 595
BseMI GCAATG 1 cut(s) 565
BseMII CTCAG 2 cut(s) 447, 697
BseNI ACTGG 3 cut(s) 171, 348, 756
BseRI GAGGAG 4 cut(s) 204, 641, 644, 647
BseXI GCAGC 1 cut(s) 416
BseYI CCCAGC 1 cut(s) 491
BshFI GGCC 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 752
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 3 cut(s) 256, 439, 750
BslFI GGGAC 2 cut(s) 13, 266
BslI CCNNNNNNNGG 3 cut(s) 177, 322, 595
BsmAI GTCTC 1 cut(s) 571
BsmFI GGGAC 2 cut(s) 13, 266
BsnI GGCC 1 cut(s) 497
Bso31I GGTCTC 1 cut(s) 571
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 317
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 1 cut(s) 469
Bsp19I CCATGG 1 cut(s) 547
BspACI CCGC 2 cut(s) 526, 768
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 2 cut(s) 446, 696
BspLI GGNNCC 4 cut(s) 254, 471, 496, 754
BspPI GGATC 2 cut(s) 464, 477
BspQI GCTCTTC 1 cut(s) 432
BspT107I GGYRCC 1 cut(s) 752
BspTNI GGTCTC 1 cut(s) 571
BsrDI GCAATG 1 cut(s) 565
BsrFI RCCGGY 1 cut(s) 749
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 3 cut(s) 171, 348, 756
BssAI RCCGGY 1 cut(s) 749
BssECI CCNNGG 3 cut(s) 438, 498, 547
BssMI GATC 1 cut(s) 469
BssT1I CCWWGG 2 cut(s) 498, 547
Bst4CI ACNGT 6 cut(s) 27, 152, 159, 242, 415, 598
Bst6I CTCTTC 2 cut(s) 432, 618
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 2 cut(s) 189, 402
BstDEI CTNAG 3 cut(s) 433, 605, 683
BstDSI CCRYGG 1 cut(s) 547
BstKTI GATC 1 cut(s) 472
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 1 cut(s) 469
BstMWI GCNNNNNNNGC 2 cut(s) 209, 694
BstSCI CCNGG 2 cut(s) 437, 438
BstV1I GCAGC 1 cut(s) 416
BstV2I GAAGAC 1 cut(s) 473
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 683
BsuRI GGCC 1 cut(s) 497
BtgI CCRYGG 1 cut(s) 547
Cac8I GCNNGC 2 cut(s) 189, 402
Cfr10I RCCGGY 1 cut(s) 749
Cfr13I GGNCC 3 cut(s) 253, 272, 495
Cfr9I CCCGGG 1 cut(s) 438
Csp6I GTAC 3 cut(s) 23, 69, 753
CviAII CATG 3 cut(s) 127, 461, 548
CviJI RGCY 9 cut(s) 315, 425, 437, 497, 592, 617, 688, 749, 776
CviKI_1 RGCY 9 cut(s) 315, 425, 437, 497, 592, 617, 688, 749, 776
CviQI GTAC 3 cut(s) 23, 69, 753
DdeI CTNAG 3 cut(s) 433, 605, 683
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 432, 618
EarI CTCTTC 2 cut(s) 432, 618
Eco130I CCWWGG 2 cut(s) 498, 547
Eco24I GRGCYC 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 571
Eco47I GGWCC 2 cut(s) 253, 272
Eco81I CCTNAGG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 495
EcoT14I CCWWGG 2 cut(s) 498, 547
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 317
ErhI CCWWGG 2 cut(s) 498, 547
FaeI CATG 3 cut(s) 130, 464, 551
FaiI YATR 6 cut(s) 66, 128, 356, 456, 462, 549
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 2 cut(s) 13, 266
FatI CATG 3 cut(s) 126, 460, 547
Fnu4HI GCNGC 1 cut(s) 405
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 1 cut(s) 405
GluI GCNGC 1 cut(s) 405
GsaI CCCAGC 1 cut(s) 495
GsuI CTGGAG 2 cut(s) 89, 225
HaeIII GGCC 1 cut(s) 497
HapII CCGG 3 cut(s) 256, 439, 750
Hin1II CATG 3 cut(s) 130, 464, 551
HincII GTYRAC 4 cut(s) 155, 328, 655, 761
HindII GTYRAC 4 cut(s) 155, 328, 655, 761
HinfI GANTC 2 cut(s) 513, 522
HpaI GTTAAC 1 cut(s) 655
HpaII CCGG 3 cut(s) 256, 439, 750
HphI GGTGA 4 cut(s) 245, 254, 255, 610
Hpy166II GTNNAC 5 cut(s) 23, 155, 328, 655, 761
Hpy188I TCNGA 3 cut(s) 99, 229, 312
Hpy188III TCNNGA 7 cut(s) 106, 270, 473, 620, 673, 691, 780
Hpy8I GTNNAC 5 cut(s) 23, 155, 328, 655, 761
HpyAV CCTTC 3 cut(s) 126, 276, 285
HpyCH4III ACNGT 6 cut(s) 27, 152, 159, 242, 415, 598
HpyCH4IV ACGT 2 cut(s) 478, 723
HpyCH4V TGCA 6 cut(s) 191, 371, 379, 385, 418, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 694
HpyF3I CTNAG 3 cut(s) 433, 605, 683
HpySE526I ACGT 2 cut(s) 478, 723
Hsp92II CATG 3 cut(s) 130, 464, 551
KpnI GGTACC 1 cut(s) 756
KspAI GTTAAC 1 cut(s) 655
Kzo9I GATC 1 cut(s) 469
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 217, 622
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 5 cut(s) 233, 358, 427, 436, 591
MaeII ACGT 2 cut(s) 478, 723
MaeIII GTNAC 2 cut(s) 388, 724
MalI GATC 1 cut(s) 471
MboI GATC 1 cut(s) 469
MboII GAAGA 6 cut(s) 66, 257, 377, 419, 473, 635
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 7 cut(s) 61, 165, 287, 303, 583, 658, 707
MmeI TCCRAC 2 cut(s) 447, 484
MnlI CCTC 5 cut(s) 225, 619, 622, 625, 692
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 3 cut(s) 296, 306, 654
MslI CAYNNNNRTG 1 cut(s) 131
MspI CCGG 3 cut(s) 256, 439, 750
MspR9I CCNGG 2 cut(s) 439, 440
MwoI GCNNNNNNNGC 2 cut(s) 209, 694
NciI CCSGG 2 cut(s) 439, 440
NcoI CCATGG 1 cut(s) 547
NdeII GATC 1 cut(s) 469
NlaIII CATG 3 cut(s) 130, 464, 551
NlaIV GGNNCC 4 cut(s) 254, 471, 496, 754
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 2 cut(s) 513, 522
PkrI GCNGC 1 cut(s) 406
PshBI ATTAAT 1 cut(s) 306
PsiI TTATAA 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 4 cut(s) 254, 471, 496, 754
PspPI GGNCC 3 cut(s) 253, 272, 495
PsrI GAACNNNNNNTAC 2 cut(s) 524, 556
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 3 cut(s) 24, 70, 754
RsaNI GTAC 3 cut(s) 23, 69, 753
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 296, 306, 654
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 1 cut(s) 469
Sau96I GGNCC 3 cut(s) 253, 272, 495
ScaI AGTACT 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 439, 440
SduI GDGCHC 1 cut(s) 317
SetI ASST 8 cut(s) 118, 268, 277, 327, 481, 594, 619, 726
SfaNI GCATC 5 cut(s) 233, 358, 427, 436, 591
SinI GGWCC 2 cut(s) 253, 272
SmaI CCCGGG 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
SrfI GCCCGGGC 1 cut(s) 440
Sse9I AATT 7 cut(s) 61, 165, 287, 303, 583, 658, 707
SsiI CCGC 2 cut(s) 526, 768
StyD4I CCNGG 2 cut(s) 437, 438
StyI CCWWGG 2 cut(s) 498, 547
TaaI ACNGT 6 cut(s) 27, 152, 159, 242, 415, 598
TaiI ACGT 2 cut(s) 481, 726
TasI AATT 7 cut(s) 61, 165, 287, 303, 583, 658, 707
TatI WGTACW 2 cut(s) 22, 68
TfiI GAWTC 2 cut(s) 513, 522
Tru1I TTAA 3 cut(s) 296, 306, 654
Tru9I TTAA 3 cut(s) 296, 306, 654
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 388
TspGWI ACGGA 1 cut(s) 26
TspMI CCCGGG 1 cut(s) 438
VpaK11BI GGWCC 2 cut(s) 253, 272
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 2 cut(s) 165, 707
XmaI CCCGGG 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.