MD06G1011100.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016302
Physical Location & Seq
Reverse (-)
1332079 .. 1336111
4033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1011100.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGTGGGTTGGCAGAGGCATGTACAGCCCCTAATCCGTCATGTTGGCAAAAGAGTGGAGAATCATGGTTACACGTCTGCTGCAAACTTTTCTTCTTCTTCTCATTTTCTAAAACTCCAGACACCATCTTACCAAACCATCTCAAGGCCCCTTCATCAGTACTTTCAACACTTGGGAATTTCCAGTTCAAGGAACTTGCTTGCAGATTCATCTGATGGAGCACCTCATCAATCTTCAGTCACTCCTCTCTCAGCTTCAAGTGGTGAAAAAATTGAAGAACAAAACCAGAAATCATCTTCTAGACCTTCACAAGTTCAATTTGTATTAAAGGGCATGAATCAGAGTCCTAATAAGGTCAACTTGGTTGCTAAACTGGTTCGTGGTATGCGTGTTGAAGATGCATTACTGCAACTGCAAGTGACAGACAAGCAAGCTGCAAAAACTGTGTATCAGGCTCTTCACTCAGCCCGGGCGGATGCAACTTATAATCATGGGTTGGATCCAGAACGTCTTCTAGTTGCTGGGGCCTTGGTTGGAAAGGGATTCTTGGGATTCCGCAAGAACAGACTTTACTACCATGGTAAAAGCAATGACGGAACTAAAGTGAGACCAAATTACCAACTAACGGTGACGCTAAGGGAGATTTATCCGGAAGAGGAGGAGGAGATAGCCAAACAGAGAGCTGACAATTTTCTCTATATCAAGAAACGCCTCCGGCTCACGAAGCAGGAAAATAAATTTGTTTCTCACCACCTTATCGGCAATTACAAAGGCAAAGGCAGTACCAGTCAACAAGGTGGTATGGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

271

Amino Acids

30.24

Weight (kDa)

9.96

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 110 - 212 7.8e-19 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00560
malus_domestica MD03G1130200.v1.1 MD06G1011100.v1.1
prunus_persica Prupe.2G035300_v2.0.a1 Prupe.7G027600_v2.0.a1
pyrus_communis pycom06g00910
rosa_chinensis RchiOBHm_Chr4g0386011
rosa_laevigata RLG00000010181
rosa_multiflora Rmu_sc0000472.1_g000020
rosa_rugosa Rorug03G0306200
rosa_samantha Rh4AG010000 Rh4BG007000 Rh4CG010700 Rh4DG008100
rosa_wichuraiana Rw4G000140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 2 cut(s) 473, 556
AclWI GGATC 2 cut(s) 494, 507
AcsI RAATTY 2 cut(s) 177, 737
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 3 cut(s) 24, 161, 784
AfiI CCNNNNNNNGG 3 cut(s) 144, 189, 625
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 6 cut(s) 167, 189, 258, 275, 317, 395
AjiI CACGTC 1 cut(s) 75
AluBI AGCT 3 cut(s) 254, 434, 683
AluI AGCT 3 cut(s) 254, 434, 683
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 2 cut(s) 494, 507
Ama87I CYCGRG 1 cut(s) 468
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 2 cut(s) 146, 525
ApeKI GCWGC 2 cut(s) 80, 434
ApoI RAATTY 2 cut(s) 177, 737
Asp700I GAANNNNTTC 1 cut(s) 510
AspS9I GGNCC 2 cut(s) 147, 525
AsuC2I CCSGG 2 cut(s) 469, 470
AsuHPI GGTGA 3 cut(s) 275, 640, 740
AvaI CYCGRG 1 cut(s) 468
BamHI GGATCC 1 cut(s) 499
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 67, 421
BccI CCATC 3 cut(s) 133, 146, 209
BcnI CCSGG 2 cut(s) 469, 470
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 2 cut(s) 300, 515
BisI GCNGC 2 cut(s) 81, 435
BlsI GCNGC 2 cut(s) 82, 436
BmcAI AGTACT 1 cut(s) 161
Bme1390I CCNGG 2 cut(s) 469, 470
BmeT110I CYCGRG 1 cut(s) 468
BmgBI CACGTC 1 cut(s) 75
BmgT120I GGNCC 2 cut(s) 147, 525
BmiI GGNNCC 3 cut(s) 149, 501, 526
BmrFI CCNGG 2 cut(s) 469, 470
BmsI GCATC 2 cut(s) 388, 466
BpiI GAAGAC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 127
BpuMI CCSGG 2 cut(s) 469, 470
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 3 cut(s) 468, 528, 577
BsaWI WCCGGW 1 cut(s) 649
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 189, 625
Bse1I ACTGG 3 cut(s) 183, 378, 786
Bse3DI GCAATG 1 cut(s) 595
BseAI TCCGGA 1 cut(s) 649
BseDI CCNNGG 3 cut(s) 468, 528, 577
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 3 cut(s) 144, 189, 625
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 2 cut(s) 264, 477
BseNI ACTGG 3 cut(s) 183, 378, 786
BseRI GAGGAG 4 cut(s) 234, 671, 674, 677
BseXI GCAGC 2 cut(s) 67, 421
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 2 cut(s) 148, 527
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 3 cut(s) 469, 650, 715
BslI CCNNNNNNNGG 3 cut(s) 144, 189, 625
BsmAI GTCTC 1 cut(s) 601
BsnI GGCC 2 cut(s) 148, 527
Bso31I GGTCTC 1 cut(s) 601
BsoBI CYCGRG 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 223
Bsp13I TCCGGA 1 cut(s) 649
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 1 cut(s) 499
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 2 cut(s) 473, 556
BspANI GGCC 2 cut(s) 148, 527
BspCNI CTCAG 2 cut(s) 263, 476
BspEI TCCGGA 1 cut(s) 649
BspHI TCATGA 1 cut(s) 809
BspLI GGNNCC 3 cut(s) 149, 501, 526
BspPI GGATC 2 cut(s) 494, 507
BspQI GCTCTTC 1 cut(s) 462
BspTNI GGTCTC 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 595
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 3 cut(s) 183, 378, 786
BssECI CCNNGG 3 cut(s) 468, 528, 577
BssMI GATC 1 cut(s) 499
BssT1I CCWWGG 2 cut(s) 528, 577
Bst4CI ACNGT 2 cut(s) 445, 628
Bst6I CTCTTC 2 cut(s) 462, 648
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 2 cut(s) 201, 432
BstDEI CTNAG 3 cut(s) 250, 463, 635
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 1 cut(s) 481
BstKTI GATC 1 cut(s) 502
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 1 cut(s) 499
BstMWI GCNNNNNNNGC 2 cut(s) 25, 724
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 467, 468
BstV1I GCAGC 2 cut(s) 67, 421
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 2 cut(s) 148, 527
BtgI CCRYGG 1 cut(s) 577
BtrI CACGTC 1 cut(s) 75
BtsCI GGATG 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 201, 432
CciI TCATGA 1 cut(s) 809
Cfr13I GGNCC 2 cut(s) 147, 525
Cfr9I CCCGGG 1 cut(s) 468
CseI GACGC 1 cut(s) 640
Csp6I GTAC 3 cut(s) 23, 160, 783
CviAII CATG 7 cut(s) 20, 41, 65, 334, 491, 578, 810
CviQI GTAC 3 cut(s) 23, 160, 783
DdeI CTNAG 3 cut(s) 250, 463, 635
DpnI GATC 1 cut(s) 501
DpnII GATC 1 cut(s) 499
Eam1104I CTCTTC 2 cut(s) 462, 648
EarI CTCTTC 2 cut(s) 462, 648
EciI GGCGGA 1 cut(s) 488
Eco130I CCWWGG 2 cut(s) 528, 577
Eco31I GGTCTC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 468
EcoO109I RGGNCCY 2 cut(s) 147, 525
EcoT14I CCWWGG 2 cut(s) 528, 577
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 528, 577
FaeI CATG 7 cut(s) 23, 44, 68, 337, 494, 581, 813
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 530, 562
FatI CATG 7 cut(s) 19, 40, 64, 333, 490, 577, 809
Fnu4HI GCNGC 2 cut(s) 81, 435
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 2 cut(s) 81, 435
FspBI CTAG 2 cut(s) 300, 515
GluI GCNGC 2 cut(s) 81, 435
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 2 cut(s) 148, 527
HapII CCGG 3 cut(s) 469, 650, 715
HgaI GACGC 1 cut(s) 640
Hin1II CATG 7 cut(s) 23, 44, 68, 337, 494, 581, 813
HincII GTYRAC 2 cut(s) 358, 791
HindII GTYRAC 2 cut(s) 358, 791
HinfI GANTC 6 cut(s) 61, 206, 337, 343, 543, 552
HpaII CCGG 3 cut(s) 469, 650, 715
HphI GGTGA 3 cut(s) 275, 640, 740
Hpy166II GTNNAC 2 cut(s) 358, 791
Hpy188I TCNGA 2 cut(s) 214, 342
Hpy188III TCNNGA 7 cut(s) 118, 300, 503, 650, 703, 721, 810
Hpy8I GTNNAC 2 cut(s) 358, 791
HpyAV CCTTC 2 cut(s) 161, 315
HpyCH4III ACNGT 2 cut(s) 445, 628
HpyCH4IV ACGT 2 cut(s) 74, 508
HpyCH4V TGCA 7 cut(s) 83, 203, 401, 409, 415, 437, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 724
HpyF3I CTNAG 3 cut(s) 250, 463, 635
HpySE526I ACGT 2 cut(s) 74, 508
Hsp92II CATG 7 cut(s) 23, 44, 68, 337, 494, 581, 813
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 1 cut(s) 499
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 2 cut(s) 67, 421
LweI GCATC 2 cut(s) 388, 466
MaeI CTAG 2 cut(s) 300, 515
MaeII ACGT 2 cut(s) 74, 508
MaeIII GTNAC 4 cut(s) 68, 238, 418, 628
MalI GATC 1 cut(s) 501
MboI GATC 1 cut(s) 499
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 7 cut(s) 177, 270, 317, 613, 688, 737, 763
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 2 cut(s) 477, 514
MnlI CCTC 7 cut(s) 9, 234, 255, 649, 652, 655, 722
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 1 cut(s) 326
MspI CCGG 3 cut(s) 469, 650, 715
MspR9I CCNGG 2 cut(s) 469, 470
MwoI GCNNNNNNNGC 2 cut(s) 25, 724
NciI CCSGG 2 cut(s) 469, 470
NcoI CCATGG 1 cut(s) 577
NdeII GATC 1 cut(s) 499
NlaIII CATG 7 cut(s) 23, 44, 68, 337, 494, 581, 813
NlaIV GGNNCC 3 cut(s) 149, 501, 526
NmuCI GTSAC 3 cut(s) 238, 418, 628
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 23
PagI TCATGA 1 cut(s) 809
PciSI GCTCTTC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 5 cut(s) 61, 206, 337, 543, 552
PkrI GCNGC 2 cut(s) 82, 436
PleI GAGTC 1 cut(s) 351
PpsI GAGTC 1 cut(s) 351
PsiI TTATAA 1 cut(s) 486
PspFI CCCAGC 1 cut(s) 521
PspN4I GGNNCC 3 cut(s) 149, 501, 526
PspPI GGNCC 2 cut(s) 147, 525
PsrI GAACNNNNNNTAC 2 cut(s) 554, 586
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 3 cut(s) 24, 161, 784
RsaNI GTAC 3 cut(s) 23, 160, 783
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 2 cut(s) 81, 435
Sau3AI GATC 1 cut(s) 499
Sau96I GGNCC 2 cut(s) 147, 525
ScaI AGTACT 1 cut(s) 161
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 469, 470
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 2 cut(s) 388, 466
SmaI CCCGGG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
SrfI GCCCGGGC 1 cut(s) 470
Sse9I AATT 7 cut(s) 177, 270, 317, 613, 688, 737, 763
SsiI CCGC 2 cut(s) 473, 556
SspMI CTAG 2 cut(s) 300, 515
StyD4I CCNGG 2 cut(s) 467, 468
StyI CCWWGG 2 cut(s) 528, 577
TaaI ACNGT 2 cut(s) 445, 628
TaiI ACGT 2 cut(s) 77, 511
TasI AATT 7 cut(s) 177, 270, 317, 613, 688, 737, 763
TatI WGTACW 2 cut(s) 22, 159
TfiI GAWTC 5 cut(s) 61, 206, 337, 543, 552
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TseFI GTSAC 3 cut(s) 238, 418, 628
TseI GCWGC 2 cut(s) 80, 434
Tsp45I GTSAC 3 cut(s) 238, 418, 628
TspDTI ATGAA 4 cut(s) 143, 198, 350, 798
TspGWI ACGGA 2 cut(s) 26, 609
TspMI CCCGGG 1 cut(s) 468
XapI RAATTY 2 cut(s) 177, 737
XbaI TCTAGA 1 cut(s) 299
XceI RCATGY 1 cut(s) 23
XmaI CCCGGG 1 cut(s) 468
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 2 cut(s) 300, 515
ZrmI AGTACT 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.