MD03G1140200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018101
Physical Location & Seq
Forward (+)
14620490 .. 14621723
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1140200.v1.1.491

Sequence Viewer

Length: 759 bp
ATGCCATGGATTTTTCAGCTAGACGAAGAGAAGTTGGAGTTCAAATCCGGAAATGATGTGCTGGAAATGTTCAAGTATGGAACCAACTACAATCTTGCATTAGACAAAGTCCTTCTCTGGAGGGATTTCTTCAAGGGGAGAGTATGGGCTGGTTTGGTATTGTTGTGCTTTGAAAAGAAACTGTTTTTGTTGTGCTGTGCTCTCCCTCAAGTTTGTGAGTGGAGCACCTTTAACACTCCCCCTCAAGTTGGAGTGTGGATATCAATAACACCCAACTACAGACATGATGCGATGAACATGGATCGCGGCCTACAAACGCTCGCCGCCAACTACTATCCGCGTTGCTCTCAGCCAGAACATGCAATTGGTATACCACATCACACTGATCATGGCCTTGTCACTCTCGTCATCCAGAATGAGATGAATGGCCTCCAAGTTGAACACAACGGCCAGTGGCTCACCGTCAATGGCCCTGCCAACGGATTCTTTGTCAACCTCGCTGACCAAATGCAAATTCTTACTAATGGCAAATACAAGAGTGTGGTGCATCGGGCAACTTTCAACAACAAAGCTTCGAGGATATCTATTGCCATACCACATGGACGATCTGTTGATACAATTAATGTACCGGCGCCGGAGTTGCTGGAAAAAGAAGGCCAAGCTCCGAAATACATTGGAATGAACTATAAGGAGTATATCCAGCGTCAACAAAGCGGCAAGAACTATATGAAATCCACCTTTGATCACATACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

253

Amino Acids

28.83

Weight (kDa)

7.69

Isoelectric Point (pI)

24.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 105 - 198 6.7e-26 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018101)

Species Orthologous Gene IDs
malus_domestica MD03G1140200.v1.1 MD09G1157200.v1.1
pyrus_communis pycom03g09530 pycom03g09540
rosa_laevigata RLG00000036004
rosa_multiflora Rmu_sc0000076.1_g000056 Rmu_sc0000376.1_g000008
rosa_roxburghii Rroxscaffold_2G00102660
rosa_samantha Rh4DG104400 Rh5BG468200 Rh5CG490300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 370
AccII CGCG 2 cut(s) 306, 340
AccIII TCCGGA 1 cut(s) 47
AciI CCGC 4 cut(s) 306, 324, 338, 714
AclWI GGATC 1 cut(s) 309
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 1 cut(s) 513
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 2 cut(s) 248, 479
AgsI TTSAA 6 cut(s) 43, 73, 133, 173, 440, 562
AjuI GAANNNNNNNTTGG 4 cut(s) 348, 380, 498, 530
AluBI AGCT 3 cut(s) 19, 572, 662
AluI AGCT 3 cut(s) 19, 572, 662
Alw21I GWGCWC 2 cut(s) 202, 227
AlwI GGATC 1 cut(s) 309
Aor13HI TCCGGA 1 cut(s) 47
AoxI GGCC 6 cut(s) 307, 391, 427, 448, 469, 655
ApoI RAATTY 1 cut(s) 513
AseI ATTAAT 1 cut(s) 621
AspLEI GCGC 1 cut(s) 634
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 451
BanI GGYRCC 1 cut(s) 631
Bbv12I GWGCWC 2 cut(s) 202, 227
BceAI ACGGC 1 cut(s) 463
BclI TGATCA 2 cut(s) 385, 742
BfaI CTAG 2 cut(s) 20, 752
BfmI CTRYAG 1 cut(s) 277
BfoI RGCGCY 1 cut(s) 635
BisI GCNGC 3 cut(s) 307, 324, 715
BlsI GCNGC 3 cut(s) 308, 325, 716
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 2 cut(s) 82, 633
BmsI GCATC 2 cut(s) 277, 556
BpmI CTGGAG 1 cut(s) 139
BpuEI CTTGAG 2 cut(s) 192, 228
BsaHI GRCGYC 1 cut(s) 632
BsaJI CCNNGG 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 47
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 479
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 1 cut(s) 451
BseAI TCCGGA 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 248, 479
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 451
Bsh1236I CGCG 2 cut(s) 306, 340
BshFI GGCC 6 cut(s) 309, 393, 429, 450, 471, 657
BshNI GGYRCC 1 cut(s) 631
BsiHKAI GWGCWC 2 cut(s) 202, 227
BsiSI CCGG 3 cut(s) 48, 629, 635
BslI CCNNNNNNNGG 2 cut(s) 248, 479
BsnI GGCC 6 cut(s) 309, 393, 429, 450, 471, 657
Bsp1286I GDGCHC 2 cut(s) 202, 227
Bsp13I TCCGGA 1 cut(s) 47
Bsp143I GATC 4 cut(s) 301, 385, 605, 742
Bsp19I CCATGG 1 cut(s) 5
BspACI CCGC 4 cut(s) 306, 324, 338, 714
BspANI GGCC 6 cut(s) 309, 393, 429, 450, 471, 657
BspCNI CTCAG 1 cut(s) 361
BspEI TCCGGA 1 cut(s) 47
BspFNI CGCG 2 cut(s) 306, 340
BspLI GGNNCC 2 cut(s) 82, 633
BspPI GGATC 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 631
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 4 cut(s) 301, 385, 605, 742
BssNAI GTATAC 1 cut(s) 371
BssNI GRCGYC 1 cut(s) 632
BssT1I CCWWGG 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 183, 463
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 632
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 2 cut(s) 306, 340
BstH2I RGCGCY 1 cut(s) 635
BstHHI GCGC 1 cut(s) 634
BstKTI GATC 4 cut(s) 304, 388, 608, 745
BstMBI GATC 4 cut(s) 301, 385, 605, 742
BstMWI GCNNNNNNNGC 1 cut(s) 640
BstNSI RCATGY 1 cut(s) 362
BstSFI CTRYAG 1 cut(s) 277
BstUI CGCG 2 cut(s) 306, 340
BstZ17I GTATAC 1 cut(s) 371
BsuRI GGCC 6 cut(s) 309, 393, 429, 450, 471, 657
BtgI CCRYGG 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 305
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 2 cut(s) 381, 458
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 1 cut(s) 634
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 470
CseI GACGC 1 cut(s) 692
Csp6I GTAC 1 cut(s) 626
CviAII CATG 6 cut(s) 6, 284, 298, 359, 389, 599
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 1 cut(s) 348
DinI GGCGCC 1 cut(s) 633
DpnI GATC 4 cut(s) 303, 387, 607, 744
DpnII GATC 4 cut(s) 301, 385, 605, 742
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 5
Eco32I GATATC 2 cut(s) 261, 582
EcoRV GATATC 2 cut(s) 261, 582
EcoT14I CCWWGG 1 cut(s) 5
EgeI GGCGCC 1 cut(s) 633
EheI GGCGCC 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 5
FaeI CATG 6 cut(s) 9, 287, 301, 362, 392, 602
FatI CATG 6 cut(s) 5, 283, 297, 358, 388, 598
FbaI TGATCA 2 cut(s) 385, 742
FblI GTMKAC 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 307, 324, 715
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 307, 324, 715
FspBI CTAG 2 cut(s) 20, 752
GlaI GCGC 1 cut(s) 633
GluI GCNGC 3 cut(s) 307, 324, 715
GsuI CTGGAG 1 cut(s) 139
HaeII RGCGCY 1 cut(s) 635
HaeIII GGCC 6 cut(s) 309, 393, 429, 450, 471, 657
HapII CCGG 3 cut(s) 48, 629, 635
HgaI GACGC 1 cut(s) 692
HhaI GCGC 1 cut(s) 634
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 6 cut(s) 9, 287, 301, 362, 392, 602
Hin6I GCGC 1 cut(s) 632
HinP1I GCGC 1 cut(s) 632
HincII GTYRAC 2 cut(s) 493, 707
HindII GTYRAC 2 cut(s) 493, 707
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 1 cut(s) 483
HpaII CCGG 3 cut(s) 48, 629, 635
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 3 cut(s) 371, 493, 707
Hpy188I TCNGA 1 cut(s) 666
Hpy188III TCNNGA 3 cut(s) 48, 118, 412
Hpy8I GTNNAC 3 cut(s) 371, 493, 707
HpyAV CCTTC 2 cut(s) 122, 647
HpyCH4III ACNGT 2 cut(s) 183, 463
HpyCH4V TGCA 4 cut(s) 98, 362, 511, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 640
HpyF3I CTNAG 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 6 cut(s) 9, 287, 301, 362, 392, 602
HspAI GCGC 1 cut(s) 632
KasI GGCGCC 1 cut(s) 631
Kpn2I TCCGGA 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 385, 742
Kzo9I GATC 4 cut(s) 301, 385, 605, 742
LmnI GCTCC 2 cut(s) 222, 667
LweI GCATC 2 cut(s) 277, 556
MaeI CTAG 2 cut(s) 20, 752
MaeIII GTNAC 1 cut(s) 397
MalI GATC 4 cut(s) 303, 387, 607, 744
MboI GATC 4 cut(s) 301, 385, 605, 742
MboII GAAGA 2 cut(s) 38, 121
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 2 cut(s) 202, 227
MluCI AATT 3 cut(s) 363, 513, 618
Mly113I GGCGCC 1 cut(s) 632
MmeI TCCRAC 2 cut(s) 15, 229
MnlI CCTC 6 cut(s) 114, 216, 252, 440, 506, 570
MroI TCCGGA 1 cut(s) 47
MseI TTAA 2 cut(s) 231, 621
MslI CAYNNNNRTG 1 cut(s) 677
MspI CCGG 3 cut(s) 48, 629, 635
MunI CAATTG 1 cut(s) 363
MvnI CGCG 2 cut(s) 306, 340
MwoI GCNNNNNNNGC 1 cut(s) 640
NarI GGCGCC 1 cut(s) 632
NcoI CCATGG 1 cut(s) 5
NdeII GATC 4 cut(s) 301, 385, 605, 742
NlaIII CATG 6 cut(s) 9, 287, 301, 362, 392, 602
NlaIV GGNNCC 2 cut(s) 82, 633
NmuCI GTSAC 1 cut(s) 397
NspI RCATGY 1 cut(s) 362
PfeI GAWTC 1 cut(s) 483
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 308, 325, 716
PluTI GGCGCC 1 cut(s) 635
PshBI ATTAAT 1 cut(s) 621
PspN4I GGNNCC 2 cut(s) 82, 633
PspPI GGNCC 1 cut(s) 470
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 2 cut(s) 231, 621
SatI GCNGC 3 cut(s) 307, 324, 715
Sau3AI GATC 4 cut(s) 301, 385, 605, 742
Sau96I GGNCC 1 cut(s) 470
SduI GDGCHC 2 cut(s) 202, 227
SetI ASST 6 cut(s) 21, 230, 498, 574, 664, 740
SfaNI GCATC 2 cut(s) 277, 556
SfcI CTRYAG 1 cut(s) 277
SfoI GGCGCC 1 cut(s) 633
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 2 cut(s) 207, 243
SmoI CTYRAG 2 cut(s) 207, 243
Sse9I AATT 3 cut(s) 363, 513, 618
SsiI CCGC 4 cut(s) 306, 324, 338, 714
SspDI GGCGCC 1 cut(s) 631
SspMI CTAG 2 cut(s) 20, 752
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 2 cut(s) 183, 463
TaqI TCGA 1 cut(s) 575
TasI AATT 3 cut(s) 363, 513, 618
TauI GCSGC 3 cut(s) 309, 326, 717
TfiI GAWTC 1 cut(s) 483
Tru1I TTAA 2 cut(s) 231, 621
Tru9I TTAA 2 cut(s) 231, 621
TscAI CASTG 2 cut(s) 388, 458
TseFI GTSAC 1 cut(s) 397
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 4 cut(s) 308, 437, 695, 743
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 2 cut(s) 388, 458
Tth111I GACNNNGTC 1 cut(s) 107
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 1 cut(s) 513
XceI RCATGY 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 370
XspI CTAG 2 cut(s) 20, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.