pycom03g09530

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0018101
Physical Location & Seq
Forward (+)
8105295 .. 8105987
693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09530.1

Sequence Viewer

Length: 693 bp
ATGTTCAAGTACGGAACCAGCTACAATCTTGCATTAGACAAAGTCCCTCTCTGGAGGGACTTCTTCAAGGTGAGAGTACATCCCGAGTTCTACTCCCTCTACAAACCGGCTTGCTTCAGTGAGGTTTCAATGGAGTTCAGCAAGAGAACAAGAGAAGTAGCATTGGAGATAACACGCGCAATCTCCGAAAGTTTGGGACTGGAGCCCAACTACATACATGATGCGATGAACATGGATCGCGGCCTACAAATGCTGGCCGCCAACTACTATCTGCCTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCACACTGATCATGGCCTTGTCACCCTCCTCATCCAGAATGAGATGAATGGCCTCCAAGTTGAACATAACGGCAAGTGGCTCACCGTCAATGGCCCTGCCAACGGTTTCTTTGTCAACCTCGCTGACCAAATGCAAATTCTTACTAATGGCAAATACAAGAGTGTGATGCATCGGGCAACGGTGAACAACAAAGCGACGAGGATATCTATTGCCATACCACGTGGACCATGTGTTGATACACTTATCGCACTGGCACCGGAGTTATTGGAAAAAGAAGGCCAAGCACCGAAATACATTGGAATGAATTATAAGGAGTATATCCAGCTTCAACAGAGCGGCAAGAACTACATGAAATCCACCTTTGATCACATACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

231

Amino Acids

26.17

Weight (kDa)

7.75

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 83 - 178 7.3e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018101)

Species Orthologous Gene IDs
malus_domestica MD03G1140200.v1.1 MD09G1157200.v1.1
pyrus_communis pycom03g09530 pycom03g09540
rosa_laevigata RLG00000036004
rosa_multiflora Rmu_sc0000076.1_g000056 Rmu_sc0000376.1_g000008
rosa_roxburghii Rroxscaffold_2G00102660
rosa_samantha Rh4DG104400 Rh5BG468200 Rh5CG490300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 648
AccI GTMKAC 1 cut(s) 304
AccII CGCG 2 cut(s) 177, 240
AciI CCGC 3 cut(s) 240, 258, 648
AclWI GGATC 1 cut(s) 243
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 100
AcvI CACGTG 1 cut(s) 533
AfaI GTAC 2 cut(s) 11, 78
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 5 cut(s) 7, 67, 129, 374, 641
AjuI GAANNNNNNNTTGG 4 cut(s) 282, 314, 432, 464
AluBI AGCT 2 cut(s) 21, 637
AluI AGCT 2 cut(s) 21, 637
AlwI GGATC 1 cut(s) 243
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 6 cut(s) 241, 255, 325, 361, 403, 589
ApoI RAATTY 1 cut(s) 447
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 2 cut(s) 404, 536
AsuHPI GGTGA 4 cut(s) 82, 325, 385, 505
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 1 cut(s) 536
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 207
BbrPI CACGTG 1 cut(s) 533
BbvCI CCTCAGC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 397
BclI TGATCA 2 cut(s) 319, 676
BfaI CTAG 1 cut(s) 686
BisI GCNGC 3 cut(s) 241, 258, 649
BlsI GCNGC 3 cut(s) 242, 259, 650
Bme18I GGWCC 1 cut(s) 536
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 404, 536
BmiI GGNNCC 3 cut(s) 16, 204, 567
BmsI GCATC 3 cut(s) 211, 468, 490
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 2 cut(s) 73, 221
Bpu10I CCTNAGC 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 533
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 2 cut(s) 204, 567
BseGI GGATG 2 cut(s) 79, 342
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 204, 567
BseRI GAGGAG 1 cut(s) 329
Bsh1236I CGCG 2 cut(s) 177, 240
BshFI GGCC 6 cut(s) 243, 257, 327, 363, 405, 591
BshNI GGYRCC 1 cut(s) 565
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 2 cut(s) 107, 569
BslFI GGGAC 3 cut(s) 29, 71, 210
BslI CCNNNNNNNGG 1 cut(s) 413
BsmFI GGGAC 3 cut(s) 29, 71, 210
BsnI GGCC 6 cut(s) 243, 257, 327, 363, 405, 591
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 3 cut(s) 235, 319, 676
BspACI CCGC 3 cut(s) 240, 258, 648
BspANI GGCC 6 cut(s) 243, 257, 327, 363, 405, 591
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 2 cut(s) 177, 240
BspLI GGNNCC 3 cut(s) 16, 204, 567
BspPI GGATC 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 2 cut(s) 204, 567
BssAI RCCGGY 1 cut(s) 106
BssMI GATC 3 cut(s) 235, 319, 676
BssNAI GTATAC 1 cut(s) 305
Bst1107I GTATAC 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 397, 416, 493
BstBAI YACGTR 1 cut(s) 533
BstC8I GCNNGC 2 cut(s) 112, 255
BstDEI CTNAG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 79, 342
BstFNI CGCG 2 cut(s) 177, 240
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 3 cut(s) 238, 322, 679
BstMBI GATC 3 cut(s) 235, 319, 676
BstNSI RCATGY 1 cut(s) 296
BstUI CGCG 2 cut(s) 177, 240
BstZ17I GTATAC 1 cut(s) 305
BsuRI GGCC 6 cut(s) 243, 257, 327, 363, 405, 591
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 2 cut(s) 79, 342
BtsIMutI CAGTG 3 cut(s) 124, 315, 560
Cac8I GCNNGC 2 cut(s) 112, 255
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 2 cut(s) 404, 536
Csp6I GTAC 2 cut(s) 10, 77
CviAII CATG 6 cut(s) 218, 232, 293, 323, 540, 661
CviQI GTAC 2 cut(s) 10, 77
DdeI CTNAG 1 cut(s) 282
DpnI GATC 3 cut(s) 237, 321, 678
DpnII GATC 3 cut(s) 235, 319, 676
EaeI YGGCCR 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 100
Eco72I CACGTG 1 cut(s) 533
Eco88I CYCGRG 1 cut(s) 83
EcoRV GATATC 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 483
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 6 cut(s) 221, 235, 296, 326, 543, 664
FaqI GGGAC 3 cut(s) 29, 71, 210
FatI CATG 6 cut(s) 217, 231, 292, 322, 539, 660
FbaI TGATCA 2 cut(s) 319, 676
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 3 cut(s) 241, 258, 649
FokI GGATG 2 cut(s) 66, 329
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 241, 258, 649
FspBI CTAG 1 cut(s) 686
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 241, 258, 649
GsuI CTGGAG 2 cut(s) 73, 221
HaeIII GGCC 6 cut(s) 243, 257, 327, 363, 405, 591
HapII CCGG 2 cut(s) 107, 569
HhaI GCGC 1 cut(s) 179
Hin1II CATG 6 cut(s) 221, 235, 296, 326, 543, 664
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HpaII CCGG 2 cut(s) 107, 569
HphI GGTGA 4 cut(s) 82, 325, 385, 505
Hpy166II GTNNAC 4 cut(s) 305, 427, 496, 536
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 3 cut(s) 52, 83, 346
Hpy8I GTNNAC 4 cut(s) 305, 427, 496, 536
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 1 cut(s) 581
HpyCH4III ACNGT 3 cut(s) 397, 416, 493
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 4 cut(s) 32, 296, 445, 481
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 6 cut(s) 221, 235, 296, 326, 543, 664
HspAI GCGC 1 cut(s) 177
Ksp22I TGATCA 2 cut(s) 319, 676
Kzo9I GATC 3 cut(s) 235, 319, 676
LmnI GCTCC 1 cut(s) 202
LweI GCATC 3 cut(s) 211, 468, 490
MaeI CTAG 1 cut(s) 686
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 1 cut(s) 331
MalI GATC 3 cut(s) 237, 321, 678
MbiI CCGCTC 1 cut(s) 648
MboI GATC 3 cut(s) 235, 319, 676
MboII GAAGA 1 cut(s) 55
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 3 cut(s) 297, 447, 616
Mph1103I ATGCAT 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 611
MspI CCGG 2 cut(s) 107, 569
MunI CAATTG 1 cut(s) 297
MvnI CGCG 2 cut(s) 177, 240
NdeII GATC 3 cut(s) 235, 319, 676
NlaIII CATG 6 cut(s) 221, 235, 296, 326, 543, 664
NlaIV GGNNCC 3 cut(s) 16, 204, 567
NmuCI GTSAC 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 483
NspI RCATGY 1 cut(s) 296
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 3 cut(s) 242, 259, 650
PmaCI CACGTG 1 cut(s) 533
PmlI CACGTG 1 cut(s) 533
Ppu21I YACGTR 1 cut(s) 533
PsiI TTATAA 1 cut(s) 621
PspCI CACGTG 1 cut(s) 533
PspN4I GGNNCC 3 cut(s) 16, 204, 567
PspPI GGNCC 2 cut(s) 404, 536
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 2 cut(s) 11, 78
RsaNI GTAC 2 cut(s) 10, 77
RseI CAYNNNNRTG 1 cut(s) 611
SatI GCNGC 3 cut(s) 241, 258, 649
Sau3AI GATC 3 cut(s) 235, 319, 676
Sau96I GGNCC 2 cut(s) 404, 536
SduI GDGCHC 1 cut(s) 207
SetI ASST 7 cut(s) 23, 72, 126, 432, 535, 639, 674
SfaNI GCATC 3 cut(s) 211, 468, 490
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 1 cut(s) 611
Sse9I AATT 3 cut(s) 297, 447, 616
SsiI CCGC 3 cut(s) 240, 258, 648
SspMI CTAG 1 cut(s) 686
TaaI ACNGT 3 cut(s) 397, 416, 493
TaiI ACGT 1 cut(s) 535
TasI AATT 3 cut(s) 297, 447, 616
TatI WGTACW 1 cut(s) 76
TauI GCSGC 3 cut(s) 243, 260, 651
TscAI CASTG 3 cut(s) 124, 322, 567
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 4 cut(s) 242, 371, 629, 677
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 3 cut(s) 124, 322, 567
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 536
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 304
XspI CTAG 1 cut(s) 686
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.