Rmu_sc0000076.1_g000056

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000076.1
OG0018101
Physical Location & Seq
Forward (+)
260815 .. 262984
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000076.1_g000056.1.cds

Sequence Viewer

Length: 927 bp
atgcataatgagatgaatggtctcgaagttgagcacaatggaaaatgggtgacagtcaatggctctcccaatggctttttcgtgaactttgctgaccaaatgcagattcttacgaacggtaagtacaagagtgtgatgcatcgcgccaccgtgaacaacaaagcgacaaggatatcgatagcaataccacatggaccatccgtagacacgatcatagtaccagcaccagagttgtgtgaaagagaaggccaagctcctaaataccttgcaatgaactacaaggagtacatacaacttcagcaaagcggcaagaactacatgaagaccctccgccgcccaccttggagccctcacctccaggagtccagaaccggcggctctgtccccagaaccggccggaaaatatacgaaccggccaccggaaatatagttaacctctctacccggtttgacgccttcctccctgcccaccgtcaccaaactccaccagaagctaaaaacggacctcagaccaccggatctgaaagatcaccaccggaaccgcactgctacgtgactttctggtcaacttccgacgactttctggtcagctacagtgactccggcagctacagtaaaatccggcaaccatttttccggcatcttttaaggatgagttacctgaacaagttgagctttgttccactcgaaacaactggcgtaggataccataggtgggtccgagatgtgcgtcaacatcttaaggctaatggaattctggaaaccatccaagagcctagtcagaacgtgctctccattgagcaagctactgcttttgaagctaaacaagctaaagccataattctcatgacaaggcacatgaatgacgcgctccaaaatgaatacctcaatgaggaagacccaaagaaaactatgggtagaacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

308

Amino Acids

35.0

Weight (kDa)

9.28

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018101)

Species Orthologous Gene IDs
malus_domestica MD03G1140200.v1.1 MD09G1157200.v1.1
pyrus_communis pycom03g09530 pycom03g09540
rosa_laevigata RLG00000036004
rosa_multiflora Rmu_sc0000076.1_g000056 Rmu_sc0000376.1_g000008
rosa_roxburghii Rroxscaffold_2G00102660
rosa_samantha Rh4DG104400 Rh5BG468200 Rh5CG490300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 562, 584
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 144, 869
AciI CCGC 5 cut(s) 306, 331, 334, 375, 542
AclWI GGATC 1 cut(s) 526
AcoI YGGCCR 2 cut(s) 394, 414
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 125, 219, 287
AfiI CCNNNNNNNGG 4 cut(s) 392, 419, 715, 892
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 1 cut(s) 818
AjnI CCWGG 1 cut(s) 357
AjuI GAANNNNNNNTTGG 4 cut(s) 62, 90, 94, 122
AloI GAACNNNNNNTCC 2 cut(s) 776, 808
AluBI AGCT 8 cut(s) 254, 494, 591, 609, 675, 806, 821, 830
AluI AGCT 8 cut(s) 254, 494, 591, 609, 675, 806, 821, 830
Alw21I GWGCWC 2 cut(s) 36, 792
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 526
AoxI GGCC 3 cut(s) 247, 394, 414
ApeKI GCWGC 1 cut(s) 606
ApoI RAATTY 1 cut(s) 753
AspLEI GCGC 2 cut(s) 146, 871
AspS9I GGNCC 3 cut(s) 194, 503, 718
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 4 cut(s) 61, 344, 467, 522
AvaII GGWCC 3 cut(s) 194, 503, 718
BanII GRGCYC 1 cut(s) 350
BbsI GAAGAC 2 cut(s) 329, 903
Bbv12I GWGCWC 2 cut(s) 36, 792
BbvI GCAGC 1 cut(s) 618
BccI CCATC 2 cut(s) 205, 773
BciT130I CCWGG 1 cut(s) 359
BciVI GTATCC 1 cut(s) 698
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 1 cut(s) 777
BfmI CTRYAG 2 cut(s) 592, 610
BfrI CTTAAG 1 cut(s) 740
BfuI GTATCC 1 cut(s) 698
BisI GCNGC 4 cut(s) 307, 334, 376, 607
BlsI GCNGC 4 cut(s) 308, 335, 377, 608
Bme1390I CCNGG 2 cut(s) 359, 445
Bme18I GGWCC 3 cut(s) 194, 503, 718
BmgT120I GGNCC 3 cut(s) 194, 503, 718
BmiI GGNNCC 3 cut(s) 347, 540, 719
BmrFI CCNGG 2 cut(s) 359, 445
BmsI GCATC 3 cut(s) 126, 148, 649
BpiI GAAGAC 2 cut(s) 329, 903
BpmI CTGGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 445
Bsa29I ATCGAT 1 cut(s) 176
BsaAI YACGTR 1 cut(s) 553
BsaHI GRCGYC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 341
BsaWI WCCGGW 3 cut(s) 419, 515, 535
BsaXI ACNNNNNCTCC 4 cut(s) 584, 614, 776, 806
Bsc4I CCNNNNNNNGG 4 cut(s) 392, 419, 715, 892
Bse118I RCCGGY 3 cut(s) 371, 392, 412
Bse1I ACTGG 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 359
BseCI ATCGAT 1 cut(s) 176
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 3 cut(s) 197, 657, 765
BseLI CCNNNNNNNGG 4 cut(s) 392, 419, 715, 892
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 700
BseX3I CGGCCG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 618
Bsh1236I CGCG 2 cut(s) 144, 869
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 3 cut(s) 249, 396, 416
BshVI ATCGAT 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 2 cut(s) 36, 792
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 4 cut(s) 392, 419, 715, 892
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 3 cut(s) 249, 396, 416
Bso31I GGTCTC 1 cut(s) 26
Bsp1286I GDGCHC 3 cut(s) 36, 350, 792
Bsp143I GATC 3 cut(s) 210, 518, 527
BspACI CCGC 5 cut(s) 306, 331, 334, 375, 542
BspANI GGCC 3 cut(s) 249, 396, 416
BspCNI CTCAG 1 cut(s) 520
BspDI ATCGAT 1 cut(s) 176
BspFNI CGCG 2 cut(s) 144, 869
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 3 cut(s) 347, 540, 719
BspPI GGATC 1 cut(s) 526
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 276
BsrFI RCCGGY 3 cut(s) 371, 392, 412
BsrI ACTGG 1 cut(s) 700
BssAI RCCGGY 3 cut(s) 371, 392, 412
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 3 cut(s) 210, 518, 527
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 6 cut(s) 55, 119, 151, 473, 596, 614
BstACI GRCGYC 1 cut(s) 453
BstAFI CTTAAG 1 cut(s) 740
BstBAI YACGTR 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 804
BstDEI CTNAG 1 cut(s) 507
BstENI CCTNNNNNAGG 1 cut(s) 890
BstF5I GGATG 3 cut(s) 197, 657, 765
BstFNI CGCG 2 cut(s) 144, 869
BstHHI GCGC 2 cut(s) 146, 871
BstKTI GATC 3 cut(s) 213, 521, 530
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 3 cut(s) 210, 518, 527
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 2 cut(s) 818, 827
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 2 cut(s) 357, 443
BstSFI CTRYAG 2 cut(s) 592, 610
BstUI CGCG 2 cut(s) 144, 869
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 2 cut(s) 329, 903
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BstZI CGGCCG 1 cut(s) 394
Bsu15I ATCGAT 1 cut(s) 176
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 3 cut(s) 249, 396, 416
BsuTUI ATCGAT 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 3 cut(s) 197, 657, 765
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 544, 601
Cac8I GCNNGC 1 cut(s) 804
CciI TCATGA 1 cut(s) 846
CfoI GCGC 2 cut(s) 146, 871
Cfr10I RCCGGY 3 cut(s) 371, 392, 412
Cfr13I GGNCC 3 cut(s) 194, 503, 718
ClaI ATCGAT 1 cut(s) 176
CseI GACGC 3 cut(s) 461, 719, 875
Csp6I GTAC 3 cut(s) 124, 218, 286
CviAII CATG 4 cut(s) 191, 319, 847, 859
CviQI GTAC 3 cut(s) 124, 218, 286
DdeI CTNAG 1 cut(s) 507
DpnI GATC 3 cut(s) 212, 520, 529
DpnII GATC 3 cut(s) 210, 518, 527
DrdI GACNNNNNNGTC 2 cut(s) 562, 584
DseDI GACNNNNNNGTC 2 cut(s) 562, 584
EaeI YGGCCR 2 cut(s) 394, 414
EagI CGGCCG 1 cut(s) 394
EciI GGCGGA 1 cut(s) 320
EclXI CGGCCG 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 341
Eco24I GRGCYC 1 cut(s) 350
Eco31I GGTCTC 1 cut(s) 26
Eco32I GATATC 1 cut(s) 174
Eco47I GGWCC 3 cut(s) 194, 503, 718
Eco52I CGGCCG 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 281
EcoNI CCTNNNNNAGG 1 cut(s) 890
EcoRI GAATTC 1 cut(s) 753
EcoRII CCWGG 1 cut(s) 357
EcoRV GATATC 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 341
EcoT22I ATGCAT 2 cut(s) 6, 141
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 1 cut(s) 341
FaeI CATG 4 cut(s) 194, 322, 850, 862
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FaqI GGGAC 1 cut(s) 368
FatI CATG 4 cut(s) 190, 318, 846, 858
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 4 cut(s) 307, 334, 376, 607
FokI GGATG 3 cut(s) 184, 664, 752
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 4 cut(s) 307, 334, 376, 607
FspBI CTAG 1 cut(s) 777
GlaI GCGC 2 cut(s) 145, 870
GluI GCNGC 4 cut(s) 307, 334, 376, 607
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 3 cut(s) 249, 396, 416
HgaI GACGC 3 cut(s) 461, 719, 875
HhaI GCGC 2 cut(s) 146, 871
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 4 cut(s) 194, 322, 850, 862
Hin6I GCGC 2 cut(s) 144, 869
HinP1I GCGC 2 cut(s) 144, 869
HincII GTYRAC 3 cut(s) 433, 567, 734
HindII GTYRAC 3 cut(s) 433, 567, 734
HinfI GANTC 3 cut(s) 106, 362, 599
HpaI GTTAAC 1 cut(s) 433
HphI GGTGA 4 cut(s) 61, 344, 467, 522
Hpy166II GTNNAC 6 cut(s) 85, 154, 205, 433, 567, 734
Hpy188I TCNGA 5 cut(s) 510, 523, 574, 722, 783
Hpy188III TCNNGA 5 cut(s) 23, 82, 366, 758, 847
Hpy8I GTNNAC 6 cut(s) 85, 154, 205, 433, 567, 734
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 2 cut(s) 239, 466
HpyCH4III ACNGT 6 cut(s) 55, 119, 151, 473, 596, 614
HpyCH4IV ACGT 2 cut(s) 552, 786
HpyCH4V TGCA 4 cut(s) 4, 103, 139, 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 818, 827
HpyF3I CTNAG 1 cut(s) 507
HpySE526I ACGT 2 cut(s) 552, 786
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 4 cut(s) 194, 322, 850, 862
HspAI GCGC 2 cut(s) 144, 869
KspAI GTTAAC 1 cut(s) 433
Kzo9I GATC 3 cut(s) 210, 518, 527
LmnI GCTCC 3 cut(s) 259, 345, 876
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 3 cut(s) 126, 148, 649
MaeI CTAG 1 cut(s) 777
MaeII ACGT 2 cut(s) 552, 786
MaeIII GTNAC 5 cut(s) 49, 473, 553, 596, 656
MalI GATC 3 cut(s) 212, 520, 529
MboI GATC 3 cut(s) 210, 518, 527
MboII GAAGA 2 cut(s) 334, 908
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 3 cut(s) 36, 350, 792
MluCI AATT 2 cut(s) 753, 840
MlyI GAGTC 2 cut(s) 371, 593
MmeI TCCRAC 1 cut(s) 597
MnlI CCTC 8 cut(s) 338, 360, 365, 446, 470, 516, 886, 896
Mph1103I ATGCAT 2 cut(s) 6, 141
MseI TTAA 3 cut(s) 432, 647, 741
MslI CAYNNNNRTG 1 cut(s) 861
MspCI CTTAAG 1 cut(s) 740
MspR9I CCNGG 2 cut(s) 359, 445
MvaI CCWGG 1 cut(s) 359
MvnI CGCG 2 cut(s) 144, 869
MwoI GCNNNNNNNGC 2 cut(s) 818, 827
NciI CCSGG 1 cut(s) 445
NdeII GATC 3 cut(s) 210, 518, 527
NlaIII CATG 4 cut(s) 194, 322, 850, 862
NlaIV GGNNCC 3 cut(s) 347, 540, 719
NmuCI GTSAC 4 cut(s) 49, 473, 553, 596
NsiI ATGCAT 2 cut(s) 6, 141
PagI TCATGA 1 cut(s) 846
PfeI GAWTC 1 cut(s) 106
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 4 cut(s) 308, 335, 377, 608
PleI GAGTC 2 cut(s) 370, 593
PpsI GAGTC 2 cut(s) 370, 593
Ppu21I YACGTR 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 3 cut(s) 347, 540, 719
PspPI GGNCC 3 cut(s) 194, 503, 718
PsrI GAACNNNNNNTAC 2 cut(s) 107, 139
PsuI RGATCY 1 cut(s) 518
RsaI GTAC 3 cut(s) 125, 219, 287
RsaNI GTAC 3 cut(s) 124, 218, 286
RseI CAYNNNNRTG 1 cut(s) 861
SaqAI TTAA 3 cut(s) 432, 647, 741
SatI GCNGC 4 cut(s) 307, 334, 376, 607
Sau3AI GATC 3 cut(s) 210, 518, 527
Sau96I GGNCC 3 cut(s) 194, 503, 718
SchI GAGTC 2 cut(s) 371, 593
ScrFI CCNGG 2 cut(s) 359, 445
SduI GDGCHC 3 cut(s) 36, 350, 792
SfaNI GCATC 3 cut(s) 126, 148, 649
SfcI CTRYAG 2 cut(s) 592, 610
SinI GGWCC 3 cut(s) 194, 503, 718
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 2 cut(s) 753, 840
SsiI CCGC 5 cut(s) 306, 331, 334, 375, 542
SspMI CTAG 1 cut(s) 777
StyD4I CCNGG 2 cut(s) 357, 443
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 6 cut(s) 55, 119, 151, 473, 596, 614
TaiI ACGT 2 cut(s) 555, 789
TaqI TCGA 3 cut(s) 24, 176, 687
TasI AATT 2 cut(s) 753, 840
TatI WGTACW 2 cut(s) 123, 285
TauI GCSGC 3 cut(s) 309, 336, 378
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 3 cut(s) 432, 647, 741
Tru9I TTAA 3 cut(s) 432, 647, 741
TscAI CASTG 2 cut(s) 551, 601
TseFI GTSAC 4 cut(s) 49, 473, 553, 596
TseI GCWGC 1 cut(s) 606
Tsp45I GTSAC 4 cut(s) 49, 473, 553, 596
TspDTI ATGAA 5 cut(s) 29, 287, 335, 875, 894
TspGWI ACGGA 2 cut(s) 190, 516
TspRI CASTG 2 cut(s) 551, 601
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 3 cut(s) 194, 503, 718
XagI CCTNNNNNAGG 1 cut(s) 890
XapI RAATTY 1 cut(s) 753
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 777
Zsp2I ATGCAT 2 cut(s) 6, 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.