MD09G1157200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0018101
Physical Location & Seq
Reverse (-)
12544160 .. 12544498
339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1157200.v1.1.491

Sequence Viewer

Length: 339 bp
ATGAATGGCCTCCAGGTCGAACACAGCGGAAAGTGGGTCACTATTGATGTCCCTCCCAACTGTTTCTTTGCCCAAATGGACATTCTAACTAACGGCAAGTACAAGAGCTTGATCCATCGGGCCACCTTGAACAACAAAGCTACAAGGATCTCTGTTGTCTTTTCGCATGGACCATCTGCGGACACAGTTATCGCACCGGCGCCGGAGTTGCTGGAAAAACACGGCCAAGCTCCCAAATATATTGGAATGAACTATATAGAATACATGAAAATTCAACTGAGCGGCAAGAGCTACCAGAAGTCAACCTTAGATCACATACGATATGAGCATGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

113

Amino Acids

12.56

Weight (kDa)

9.04

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 2 - 57 4.5e-08 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018101)

Species Orthologous Gene IDs
malus_domestica MD03G1140200.v1.1 MD09G1157200.v1.1
pyrus_communis pycom03g09530 pycom03g09540
rosa_laevigata RLG00000036004
rosa_multiflora Rmu_sc0000076.1_g000056 Rmu_sc0000376.1_g000008
rosa_roxburghii Rroxscaffold_2G00102660
rosa_samantha Rh4DG104400 Rh5BG468200 Rh5CG490300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 282
AciI CCGC 3 cut(s) 27, 179, 282
AclWI GGATC 2 cut(s) 106, 155
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 270
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 1 cut(s) 101
AgsI TTSAA 2 cut(s) 130, 275
AjnI CCWGG 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 66, 98
AluBI AGCT 4 cut(s) 108, 140, 230, 291
AluI AGCT 4 cut(s) 108, 140, 230, 291
AlwI GGATC 2 cut(s) 106, 155
AoxI GGCC 3 cut(s) 7, 120, 223
ApoI RAATTY 1 cut(s) 270
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 2 cut(s) 120, 170
AvaII GGWCC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 199
BccI CCATC 2 cut(s) 123, 181
BceAI ACGGC 2 cut(s) 109, 238
BciT130I CCWGG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 203
BisI GCNGC 1 cut(s) 283
BlsI GCNGC 1 cut(s) 284
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 120, 170
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 196
BseBI CCWGG 1 cut(s) 14
BseMII CTCAG 1 cut(s) 269
BshFI GGCC 3 cut(s) 9, 122, 225
BshNI GGYRCC 1 cut(s) 199
BsiSI CCGG 2 cut(s) 197, 203
BslFI GGGAC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 3 cut(s) 9, 122, 225
Bsp143I GATC 3 cut(s) 111, 147, 310
BspACI CCGC 3 cut(s) 27, 179, 282
BspANI GGCC 3 cut(s) 9, 122, 225
BspCNI CTCAG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 2 cut(s) 106, 155
BspT107I GGYRCC 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 196
BssMI GATC 3 cut(s) 111, 147, 310
BssNI GRCGYC 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 2 cut(s) 62, 187
BstACI GRCGYC 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 330, 334
BstDEI CTNAG 2 cut(s) 278, 307
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 3 cut(s) 114, 150, 313
BstMBI GATC 3 cut(s) 111, 147, 310
BstMWI GCNNNNNNNGC 2 cut(s) 208, 288
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
BsuRI GGCC 3 cut(s) 9, 122, 225
Cac8I GCNNGC 2 cut(s) 330, 334
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 120, 170
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 167, 265, 329
CviJI RGCY 8 cut(s) 9, 108, 122, 140, 225, 230, 291, 336
CviKI_1 RGCY 8 cut(s) 9, 108, 122, 140, 225, 230, 291, 336
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 278, 307
DinI GGCGCC 1 cut(s) 201
DpnI GATC 3 cut(s) 113, 149, 312
DpnII GATC 3 cut(s) 111, 147, 310
EaeI YGGCCR 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 12
EgeI GGCGCC 1 cut(s) 201
EheI GGCGCC 1 cut(s) 201
FaeI CATG 3 cut(s) 170, 268, 332
FaiI YATR 8 cut(s) 168, 240, 255, 257, 266, 317, 324, 330
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 166, 264, 328
Fnu4HI GCNGC 1 cut(s) 283
Fsp4HI GCNGC 1 cut(s) 283
GlaI GCGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 3 cut(s) 9, 122, 225
HapII CCGG 2 cut(s) 197, 203
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 3 cut(s) 170, 268, 332
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HpaII CCGG 2 cut(s) 197, 203
Hpy166II GTNNAC 1 cut(s) 303
Hpy8I GTNNAC 1 cut(s) 303
HpyCH4III ACNGT 2 cut(s) 62, 187
HpyCH4V TGCA 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 288
HpyF3I CTNAG 2 cut(s) 278, 307
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 3 cut(s) 170, 268, 332
HspAI GCGC 1 cut(s) 200
KasI GGCGCC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 111, 147, 310
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 6 cut(s) 26, 197, 210, 216, 308, 318
MaeIII GTNAC 1 cut(s) 37
MalI GATC 3 cut(s) 113, 149, 312
MbiI CCGCTC 1 cut(s) 282
MboI GATC 3 cut(s) 111, 147, 310
MflI RGATCY 1 cut(s) 147
MluCI AATT 1 cut(s) 270
Mly113I GGCGCC 1 cut(s) 200
MnlI CCTC 2 cut(s) 20, 63
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 2 cut(s) 197, 203
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 2 cut(s) 208, 288
NarI GGCGCC 1 cut(s) 200
NdeII GATC 3 cut(s) 111, 147, 310
NlaIII CATG 3 cut(s) 170, 268, 332
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 332
PaeI GCATGC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 284
PluTI GGCGCC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 2 cut(s) 120, 170
PsuI RGATCY 1 cut(s) 147
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 1 cut(s) 283
Sau3AI GATC 3 cut(s) 111, 147, 310
Sau96I GGNCC 2 cut(s) 120, 170
ScrFI CCNGG 1 cut(s) 14
SetI ASST 7 cut(s) 18, 110, 128, 142, 232, 293, 308
SfoI GGCGCC 1 cut(s) 201
SgrAI CRCCGGYG 1 cut(s) 196
SinI GGWCC 1 cut(s) 170
SphI GCATGC 1 cut(s) 332
Sse9I AATT 1 cut(s) 270
SsiI CCGC 3 cut(s) 27, 179, 282
SspDI GGCGCC 1 cut(s) 199
StyD4I CCNGG 1 cut(s) 12
TaaI ACNGT 2 cut(s) 62, 187
TaqI TCGA 1 cut(s) 18
TasI AATT 1 cut(s) 270
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 285
TseFI GTSAC 1 cut(s) 37
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 3 cut(s) 17, 263, 281
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 1 cut(s) 270
XceI RCATGY 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.