Rh5CG490300

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0018101
Physical Location & Seq
Reverse (-)
68327669 .. 68328460
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG490300.1

Sequence Viewer

Length: 468 bp
ATGAATATGGATCGGGGCTTACAAATGCTAGCCGGGAACTACTACCCTCTTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCATACAGATCATGGTCTAGTGACACTTCTCATTCAGAATGAGATGAAGGGTCTCCAAGTCGAACACAATGGGAAATGGCTCACTGTCGATGGCCCTGCAAACGGTTTTTTTGTTAATCTTGCTGACCAAATGCAGATTCTTACAAACGGAAAGTACAAGAGTGTGATGCACCGGGCAATTGTGAATAACAAAGCTGCAAGGATATCTATAGCCATACCACATGGACCATCTGTAGATACAACTATTGCACCTTCACCAGAATTGTGTGACAGAGAAGGTCAGGCTCCAAAATACCTTGGAATGAACTACAAGGAATACATACAACTTCAACAAAGTGGCAAGAATTACATGAAGTCAACCTTCGATCACATACGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

155

Amino Acids

17.35

Weight (kDa)

7.84

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 8 - 102 4.4e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018101)

Species Orthologous Gene IDs
malus_domestica MD03G1140200.v1.1 MD09G1157200.v1.1
pyrus_communis pycom03g09530 pycom03g09540
rosa_laevigata RLG00000036004
rosa_multiflora Rmu_sc0000076.1_g000056 Rmu_sc0000376.1_g000008
rosa_roxburghii Rroxscaffold_2G00102660
rosa_samantha Rh4DG104400 Rh5BG468200 Rh5CG490300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 79
AclWI GGATC 1 cut(s) 18
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 416
AjuI GAANNNNNNNTTGG 4 cut(s) 57, 89, 207, 239
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 18
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 179, 311
AsuC2I CCSGG 2 cut(s) 34, 260
AsuHPI GGTGA 1 cut(s) 333
AsuNHI GCTAGC 1 cut(s) 28
AvaII GGWCC 1 cut(s) 311
BbvCI CCTCAGC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 268
BccI CCATC 2 cut(s) 170, 322
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BcnI CCSGG 2 cut(s) 34, 260
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 3 cut(s) 29, 104, 466
BfmI CTRYAG 2 cut(s) 294, 318
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 2 cut(s) 34, 260
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 179, 311
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 2 cut(s) 34, 260
BmsI GCATC 1 cut(s) 243
BmtI GCTAGC 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 57
BpuMI CCSGG 2 cut(s) 34, 260
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 382
Bsc4I CCNNNNNNNGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 268
BshFI GGCC 1 cut(s) 180
BsiSI CCGG 2 cut(s) 33, 259
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 143
BsnI GGCC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 143
Bsp143I GATC 3 cut(s) 10, 94, 451
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 372
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 382
BssMI GATC 3 cut(s) 10, 94, 451
BssNAI GTATAC 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 382
Bst1107I GTATAC 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 172, 191
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 57
BstKTI GATC 3 cut(s) 13, 97, 454
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 3 cut(s) 10, 94, 451
BstNSI RCATGY 1 cut(s) 71
BstSCI CCNGG 2 cut(s) 32, 258
BstSFI CTRYAG 2 cut(s) 294, 318
BstV1I GCAGC 1 cut(s) 268
BstZ17I GTATAC 1 cut(s) 80
BsuRI GGCC 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 179, 311
Csp6I GTAC 1 cut(s) 241
CviAII CATG 4 cut(s) 68, 98, 308, 436
CviJI RGCY 8 cut(s) 18, 32, 61, 166, 180, 281, 299, 371
CviKI_1 RGCY 8 cut(s) 18, 32, 61, 166, 180, 281, 299, 371
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 57
DpnI GATC 3 cut(s) 12, 96, 453
DpnII GATC 3 cut(s) 10, 94, 451
Eco130I CCWWGG 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 311
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 4 cut(s) 71, 101, 311, 439
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FatI CATG 4 cut(s) 67, 97, 307, 435
FblI GTMKAC 1 cut(s) 79
Fnu4HI GCNGC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 3 cut(s) 29, 104, 466
GluI GCNGC 1 cut(s) 282
HaeIII GGCC 1 cut(s) 180
HapII CCGG 2 cut(s) 33, 259
Hin1II CATG 4 cut(s) 71, 101, 311, 439
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 2 cut(s) 223, 462
HpaII CCGG 2 cut(s) 33, 259
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 2 cut(s) 80, 444
Hpy188I TCNGA 1 cut(s) 123
Hpy8I GTNNAC 2 cut(s) 80, 444
HpyAV CCTTC 4 cut(s) 127, 348, 356, 457
HpyCH4III ACNGT 2 cut(s) 172, 191
HpyCH4V TGCA 6 cut(s) 71, 185, 220, 256, 284, 335
HpyF3I CTNAG 1 cut(s) 57
Hsp92II CATG 4 cut(s) 71, 101, 311, 439
Kzo9I GATC 3 cut(s) 10, 94, 451
LmnI GCTCC 1 cut(s) 376
LpnPI CCDG 6 cut(s) 46, 75, 195, 272, 353, 357
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 1 cut(s) 243
MaeI CTAG 3 cut(s) 29, 104, 466
MaeIII GTNAC 2 cut(s) 106, 353
MalI GATC 3 cut(s) 12, 96, 453
MboI GATC 3 cut(s) 10, 94, 451
MfeI CAATTG 2 cut(s) 72, 264
MluCI AATT 4 cut(s) 72, 264, 347, 430
MnlI CCTC 2 cut(s) 57, 66
MseI TTAA 1 cut(s) 201
MspI CCGG 2 cut(s) 33, 259
MspR9I CCNGG 2 cut(s) 34, 260
MunI CAATTG 2 cut(s) 72, 264
NciI CCSGG 2 cut(s) 34, 260
NdeII GATC 3 cut(s) 10, 94, 451
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 4 cut(s) 71, 101, 311, 439
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 2 cut(s) 106, 353
NspI RCATGY 1 cut(s) 71
PfeI GAWTC 1 cut(s) 223
PkrI GCNGC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 2 cut(s) 179, 311
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 3 cut(s) 10, 94, 451
Sau96I GGNCC 2 cut(s) 179, 311
ScrFI CCNGG 2 cut(s) 34, 260
SetI ASST 5 cut(s) 283, 340, 367, 384, 449
SfaNI GCATC 1 cut(s) 243
SfcI CTRYAG 2 cut(s) 294, 318
SinI GGWCC 1 cut(s) 311
Sse9I AATT 4 cut(s) 72, 264, 347, 430
SspMI CTAG 3 cut(s) 29, 104, 466
StyD4I CCNGG 2 cut(s) 32, 258
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 2 cut(s) 172, 191
TaqI TCGA 3 cut(s) 147, 174, 450
TasI AATT 4 cut(s) 72, 264, 347, 430
TatI WGTACW 1 cut(s) 240
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 106, 353
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 2 cut(s) 106, 353
TspDTI ATGAA 4 cut(s) 17, 146, 404, 452
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 311
XceI RCATGY 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 79
XspI CTAG 3 cut(s) 29, 104, 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.