MD03G1161500.v1.1

Protein of unknown function (DUF3148)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014319
Physical Location & Seq
Forward (+)
19485424 .. 19486274
851 bp
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UTR
Exon/CDS
Intron
MD03G1161500.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCATTATCCTTCTTCTCTTCCACAACTGCAGTTACATGCTCAAAGCTCCATCACAATCCAGCCCTTCAATTCAACACCATGAACTCCCATAAACAAAGCTCGAATGCCGTCTGTGCCATGGCGGAGCCACCACCAGAGTCTGGGTTGCCGGCAAGGAAATCCAACCTTGCCGTAGGCTCTCCAATAATCGTAACTGAAGCTCCCAGAATGATAAAAACTGCAGCATCCGTTCCATGCCTCCGGGTTAACTCCGGCTTAGTCAAACCGGGTGACGTCGGAAGAATTGTGTCAAGAAAGCCAAAGGATGTGTGGGCAGTACGGCTTTCAATTGGCACTTACCTCATTGATGGGAAATACTTCAAGCCCTTGGAGTTGGACCTTGACCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3148

Protein Analysis

131

Amino Acids

13.99

Weight (kDa)

9.51

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRR42-like PF11347 59 - 121 6.2e-25 Protein CHLORORESPIRATORY REDUCTION 42-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20935 AT5G20935
fragaria_vesca FvH4_3g26950
malus_domestica MD03G1161500.v1.1
prunus_persica Prupe.6G149700_v2.0.a1 Prupe.6G149700_v2.0.a1
pyrus_communis pycom03g11380
rosa_chinensis RchiOBHm_Chr5g0049391
rosa_laevigata RLG00000034616
rosa_multiflora Rmu_sc0001307.1_g000005 Rmu_sc0014052.1_g000011
rosa_roxburghii Rroxscaffold_1G00032300
rosa_rugosa Rorug05G0248200
rosa_samantha Rh5AG324600 Rh5CG360400 Rh5DG348000
rosa_wichuraiana Rw5G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB7I CCANNNNNTGG 1 cut(s) 143
AciI CCGC 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 143
AgsI TTSAA 4 cut(s) 71, 76, 330, 364
AluBI AGCT 3 cut(s) 49, 102, 203
AluI AGCT 3 cut(s) 49, 102, 203
AlwNI CAGNNNCTG 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 2 cut(s) 245, 270
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 236
BccI CCATC 2 cut(s) 60, 344
BceAI ACGGC 3 cut(s) 95, 158, 338
BcnI CCSGG 2 cut(s) 245, 270
BfmI CTRYAG 2 cut(s) 30, 222
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 245, 270
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 2 cut(s) 245, 270
BmsI GCATC 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 245, 270
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 120, 369
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse118I RCCGGY 1 cut(s) 151
BseDI CCNNGG 2 cut(s) 120, 369
BseGI GGATG 2 cut(s) 227, 313
BseLI CCNNNNNNNGG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 236
BsiSI CCGG 4 cut(s) 152, 244, 255, 269
BslI CCNNNNNNNGG 1 cut(s) 143
BsmI GAATGC 1 cut(s) 112
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 129
BspMAI CTGCAG 2 cut(s) 34, 226
BsrFI RCCGGY 1 cut(s) 151
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 120, 369
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 120, 369
Bst6I CTCTTC 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 259
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 227, 313
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 2 cut(s) 243, 268
BstSFI CTRYAG 2 cut(s) 30, 222
BstV1I GCAGC 1 cut(s) 236
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 2 cut(s) 227, 313
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 320
CviAII CATG 4 cut(s) 39, 82, 121, 237
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 120, 369
Eco47I GGWCC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 219
EcoT14I CCWWGG 2 cut(s) 120, 369
ErhI CCWWGG 2 cut(s) 120, 369
FaeI CATG 4 cut(s) 42, 85, 124, 240
FaiI YATR 5 cut(s) 40, 83, 93, 122, 238
FatI CATG 4 cut(s) 38, 81, 120, 236
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 2 cut(s) 214, 320
Fsp4HI GCNGC 1 cut(s) 225
GluI GCNGC 1 cut(s) 225
HapII CCGG 4 cut(s) 152, 244, 255, 269
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 4 cut(s) 42, 85, 124, 240
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 1 cut(s) 140
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 4 cut(s) 152, 244, 255, 269
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 22, 77
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 32, 224
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 1 cut(s) 259
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 4 cut(s) 42, 85, 124, 240
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
KspAI GTTAAC 1 cut(s) 250
LmnI GCTCC 3 cut(s) 54, 127, 208
LpnPI CCDG 8 cut(s) 75, 129, 150, 165, 220, 257, 268, 282
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 236
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 3 cut(s) 34, 193, 272
MboII GAAGA 3 cut(s) 7, 12, 294
MfeI CAATTG 1 cut(s) 330
MluCI AATT 3 cut(s) 71, 285, 330
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 3 cut(s) 189, 259, 357
MnlI CCTC 2 cut(s) 251, 353
MroNI GCCGGC 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 359
MseI TTAA 1 cut(s) 249
MspI CCGG 4 cut(s) 152, 244, 255, 269
MspR9I CCNGG 2 cut(s) 245, 270
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 116
NaeI GCCGGC 1 cut(s) 153
NciI CCSGG 2 cut(s) 245, 270
NcoI CCATGG 1 cut(s) 120
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 4 cut(s) 42, 85, 124, 240
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 272
NspI RCATGY 1 cut(s) 42
PctI GAATGC 1 cut(s) 112
PdiI GCCGGC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 143
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 379
PstI CTGCAG 2 cut(s) 34, 226
PstNI CAGNNNCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 1 cut(s) 225
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 245, 270
SetI ASST 7 cut(s) 51, 104, 171, 205, 279, 345, 384
SfaNI GCATC 1 cut(s) 236
SfcI CTRYAG 2 cut(s) 30, 222
SinI GGWCC 1 cut(s) 379
Sse9I AATT 3 cut(s) 71, 285, 330
SsiI CCGC 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 243, 268
StyI CCWWGG 2 cut(s) 120, 369
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 104
TasI AATT 3 cut(s) 71, 285, 330
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 98
TspGWI ACGGA 1 cut(s) 220
Van91I CCANNNNNTGG 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 379
XceI RCATGY 1 cut(s) 42
XcmI CCANNNNNNNNNTGG 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 359
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.