RLG00000034616

Protein of unknown function (DUF3148)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014319
Physical Location & Seq
Forward (+)
46261585 .. 46262496
912 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034616

Sequence Viewer

Length: 603 bp
ATGGCTTTATCTCTCTCTTCCACTACTGCATTTACATGTTCACAGCTTTATGACAACAAGTCAGGACTGCAGTTCACCAGCTTAAGCTCTCACAAACGTAACCCAAATGCTGTCAATGTTAAGTGTGAATCGACGGAGTCAGAGTTGCCTGCAAATGCAAAGAAATCCAAGCTTGCAATTGGGTCTCCTGTTATTGTGGTCGAGGCTCCGAGAATGATAAAAACCGCAGCATCAGTTCCATGCCTCAGGGTTAACTCTGGCTTAGTCAAGCCCGGTGATGTTGGAAGAATTGTGTCAAGAAAGCCTAAGGATGTGTGGGCGGTACGTCTTACGATTGGCACTTACCTCATAGATGGGAACAAGGCTAGTGTGGCTTTTAATTCTGATATTCTGAGTTCTACTGCAAACGCCCTTGGTCCTAGAAGCAATCATAAGGGGAATTCGAAGCCGAATAGTAATGAATGTGTGCCCTGTTCATTCGGTTTGTCCCTTCTAGCTCCAAATGCCCGTCACCATCATCTTCTTTCTTGTAGTGTTGGAAAGCCTGATCAAACTCTTGTTGATACGGCTTTGCAGCTTGGCTGGGAGTTGATCCTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3148

Protein Analysis

201

Amino Acids

21.24

Weight (kDa)

9.37

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRR42-like PF11347 60 - 120 9e-24 Protein CHLORORESPIRATORY REDUCTION 42-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20935 AT5G20935
fragaria_vesca FvH4_3g26950
malus_domestica MD03G1161500.v1.1
prunus_persica Prupe.6G149700_v2.0.a1 Prupe.6G149700_v2.0.a1
pyrus_communis pycom03g11380
rosa_chinensis RchiOBHm_Chr5g0049391
rosa_laevigata RLG00000034616
rosa_multiflora Rmu_sc0001307.1_g000005 Rmu_sc0014052.1_g000011
rosa_roxburghii Rroxscaffold_1G00032300
rosa_rugosa Rorug05G0248200
rosa_samantha Rh5AG324600 Rh5CG360400 Rh5DG348000
rosa_wichuraiana Rw5G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 225, 320
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 1 cut(s) 324
AflII CTTAAG 1 cut(s) 82
AflIII ACRYGT 1 cut(s) 35
AhdI GACNNNNNGTC 1 cut(s) 58
AluBI AGCT 6 cut(s) 46, 81, 87, 172, 497, 577
AluI AGCT 6 cut(s) 46, 81, 87, 172, 497, 577
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 1 cut(s) 586
ApeKI GCWGC 2 cut(s) 227, 574
ApoI RAATTY 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 3 cut(s) 67, 287, 503
AsuII TTCGAA 1 cut(s) 443
AvaII GGWCC 1 cut(s) 416
AxyI CCTNAGG 2 cut(s) 245, 306
BaeGI GKGCMC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 239, 586
BccI CCATC 2 cut(s) 347, 522
BceAI ACGGC 1 cut(s) 582
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 3 cut(s) 366, 420, 494
BfmI CTRYAG 1 cut(s) 68
BfrI CTTAAG 1 cut(s) 82
BisI GCNGC 2 cut(s) 228, 575
BlsI GCNGC 2 cut(s) 229, 576
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 1 cut(s) 416
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 1 cut(s) 239
Bpu14I TTCGAA 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse21I CCTNAGG 2 cut(s) 245, 306
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 259, 383
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 2 cut(s) 239, 586
BseYI CCCAGC 1 cut(s) 582
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 189
BsmFI GGGAC 1 cut(s) 472
Bso31I GGTCTC 1 cut(s) 189
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 2 cut(s) 547, 591
BspACI CCGC 2 cut(s) 225, 320
BspCNI CTCAG 2 cut(s) 258, 384
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 72
BspPI GGATC 1 cut(s) 586
BspT104I TTCGAA 1 cut(s) 443
BspTI CTTAAG 1 cut(s) 82
BspTNI GGTCTC 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 2 cut(s) 547, 591
BssT1I CCWWGG 1 cut(s) 412
Bst6I CTCTTC 1 cut(s) 22
BstAFI CTTAAG 1 cut(s) 82
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 2 cut(s) 150, 174
BstDEI CTNAG 4 cut(s) 245, 262, 306, 392
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 2 cut(s) 550, 594
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 2 cut(s) 547, 591
BstMWI GCNNNNNNNGC 2 cut(s) 371, 503
BstNSI RCATGY 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 239, 586
Bsu36I CCTNAGG 2 cut(s) 245, 306
BtsCI GGATG 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 150, 174
Cfr13I GGNCC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 323
CviAII CATG 2 cut(s) 36, 240
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 4 cut(s) 245, 262, 306, 392
DpnI GATC 2 cut(s) 549, 593
DpnII GATC 2 cut(s) 547, 591
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 412
Eco31I GGTCTC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 416
Eco81I CCTNAGG 2 cut(s) 245, 306
EcoRI GAATTC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 2 cut(s) 39, 243
FaiI YATR 5 cut(s) 37, 51, 241, 350, 432
FaqI GGGAC 1 cut(s) 472
FatI CATG 2 cut(s) 35, 239
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 228, 575
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 228, 575
FspBI CTAG 3 cut(s) 366, 420, 494
GluI GCNGC 2 cut(s) 228, 575
GsaI CCCAGC 1 cut(s) 586
HapII CCGG 1 cut(s) 273
Hin1II CATG 2 cut(s) 39, 243
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 128, 137
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 1 cut(s) 273
HphI GGTGA 3 cut(s) 67, 287, 503
Hpy166II GTNNAC 3 cut(s) 41, 75, 253
Hpy188I TCNGA 4 cut(s) 142, 210, 385, 393
Hpy188III TCNNGA 2 cut(s) 63, 297
Hpy8I GTNNAC 3 cut(s) 41, 75, 253
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 1 cut(s) 500
HpyCH4IV ACGT 2 cut(s) 97, 325
HpyCH4V TGCA 7 cut(s) 29, 70, 152, 158, 176, 404, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 503
HpyF3I CTNAG 4 cut(s) 245, 262, 306, 392
HpySE526I ACGT 2 cut(s) 97, 325
Hsp92II CATG 2 cut(s) 39, 243
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 2 cut(s) 547, 591
LmnI GCTCC 2 cut(s) 211, 502
Lsp1109I GCAGC 2 cut(s) 239, 586
LweI GCATC 1 cut(s) 239
MaeI CTAG 3 cut(s) 366, 420, 494
MaeII ACGT 2 cut(s) 97, 325
MaeIII GTNAC 2 cut(s) 98, 509
MalI GATC 2 cut(s) 549, 593
MboI GATC 2 cut(s) 547, 591
MboII GAAGA 3 cut(s) 9, 297, 512
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 4 cut(s) 177, 288, 379, 439
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 2 cut(s) 262, 517
MnlI CCTC 3 cut(s) 196, 254, 356
MseI TTAA 5 cut(s) 83, 120, 252, 378, 601
MslI CAYNNNNRTG 1 cut(s) 34
MspCI CTTAAG 1 cut(s) 82
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 1 cut(s) 273
MunI CAATTG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 371, 503
NciI CCSGG 1 cut(s) 273
NdeII GATC 2 cut(s) 547, 591
NlaIII CATG 2 cut(s) 39, 243
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 509
NspI RCATGY 1 cut(s) 39
NspV TTCGAA 1 cut(s) 443
PciI ACATGT 1 cut(s) 35
PfeI GAWTC 1 cut(s) 128
PflFI GACNNNGTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 229, 576
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PscI ACATGT 1 cut(s) 35
PspFI CCCAGC 1 cut(s) 582
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 416
PstI CTGCAG 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 136
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 34
SaqAI TTAA 5 cut(s) 83, 120, 252, 378, 601
SatI GCNGC 2 cut(s) 228, 575
Sau3AI GATC 2 cut(s) 547, 591
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 273
SduI GDGCHC 1 cut(s) 471
SetI ASST 9 cut(s) 48, 83, 89, 100, 174, 328, 348, 499, 579
SfaNI GCATC 1 cut(s) 239
SfcI CTRYAG 1 cut(s) 68
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 4 cut(s) 177, 288, 379, 439
SsiI CCGC 2 cut(s) 225, 320
SspMI CTAG 3 cut(s) 366, 420, 494
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 1 cut(s) 412
TaiI ACGT 2 cut(s) 100, 328
TaqI TCGA 3 cut(s) 131, 201, 443
TasI AATT 4 cut(s) 177, 288, 379, 439
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 5 cut(s) 83, 120, 252, 378, 601
Tru9I TTAA 5 cut(s) 83, 120, 252, 378, 601
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 2 cut(s) 227, 574
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 2 cut(s) 465, 474
TspGWI ACGGA 1 cut(s) 149
Tth111I GACNNNGTC 1 cut(s) 136
Vha464I CTTAAG 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 416
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 39
XspI CTAG 3 cut(s) 366, 420, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.