RchiOBHm_Chr5g0049391

Protein of unknown function (DUF3148)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014319
Physical Location & Seq
Forward (+)
46951386 .. 46952740
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32706

Sequence Viewer

Length: 384 bp
ATGGCATTATCTCTCTCTTCCACTACTGCATTTACATGTTCACAGCTTTATGACAACAAGTCAGGACCTGGTGAATTAAGCTCTCACAAACGTAACCCAAATGCTGTCAATGTTAAGTGTGAATCGACGGAGTCAGAGTTGTCTGCAAATGCAAAGAAATCCAAGCTTGCAATTGGGTCTCCTGTTATTGTGGTCGAGGCTCCGAGAATGATAAAAACCGCAGCATCAGTTCCATGCCTCAGGGTTAACTCTGGCTTAGTCAAGCCTGGTGATGTTGGAAGAATTGTGTCAAGAAAGCCTAAGGATGTGTGGGCGGTACGTCTTACGATTGGCACTTACCTCATAGATGGGAAGTACTTCAAGCCCTTAGAGCTTGATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3148

Protein Analysis

127

Amino Acids

13.62

Weight (kDa)

9.51

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRR42-like PF11347 58 - 120 1.4e-25 Protein CHLORORESPIRATORY REDUCTION 42-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20935 AT5G20935
fragaria_vesca FvH4_3g26950
malus_domestica MD03G1161500.v1.1
prunus_persica Prupe.6G149700_v2.0.a1 Prupe.6G149700_v2.0.a1
pyrus_communis pycom03g11380
rosa_chinensis RchiOBHm_Chr5g0049391
rosa_laevigata RLG00000034616
rosa_multiflora Rmu_sc0001307.1_g000005 Rmu_sc0014052.1_g000011
rosa_roxburghii Rroxscaffold_1G00032300
rosa_rugosa Rorug05G0248200
rosa_samantha Rh5AG324600 Rh5CG360400 Rh5DG348000
rosa_wichuraiana Rw5G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 219, 314
AclWI GGATC 1 cut(s) 371
AfaI GTAC 2 cut(s) 318, 356
AflIII ACRYGT 1 cut(s) 35
AgsI TTSAA 1 cut(s) 361
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 2 cut(s) 67, 265
AluBI AGCT 4 cut(s) 46, 81, 166, 373
AluI AGCT 4 cut(s) 46, 81, 166, 373
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 83, 281
AvaII GGWCC 1 cut(s) 65
AxyI CCTNAGG 2 cut(s) 239, 300
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 341
BciT130I CCWGG 2 cut(s) 69, 267
BcoDI GTCTC 1 cut(s) 183
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 69, 267
Bme18I GGWCC 1 cut(s) 65
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 69, 267
BmsI GCATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse21I CCTNAGG 2 cut(s) 239, 300
BseBI CCWGG 2 cut(s) 69, 267
BseGI GGATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 183
Bso31I GGTCTC 1 cut(s) 183
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 2 cut(s) 219, 314
BspCNI CTCAG 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 183
BssMI GATC 1 cut(s) 376
Bst2UI CCWGG 2 cut(s) 69, 267
Bst6I CTCTTC 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 4 cut(s) 239, 256, 300, 367
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 2 cut(s) 69, 267
BstNSI RCATGY 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 67, 265
BstV1I GCAGC 1 cut(s) 233
Bsu36I CCTNAGG 2 cut(s) 239, 300
BtsCI GGATG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 168
CaiI CAGNNNCTG 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 67
Csp6I GTAC 2 cut(s) 317, 355
CviAII CATG 2 cut(s) 36, 234
CviJI RGCY 9 cut(s) 46, 81, 166, 200, 255, 265, 298, 364, 373
CviKI_1 RGCY 9 cut(s) 46, 81, 166, 200, 255, 265, 298, 364, 373
CviQI GTAC 2 cut(s) 317, 355
DdeI CTNAG 4 cut(s) 239, 256, 300, 367
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 22
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 65
Eco81I CCTNAGG 2 cut(s) 239, 300
EcoO109I RGGNCCY 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 67, 265
FaeI CATG 2 cut(s) 39, 237
FaiI YATR 4 cut(s) 37, 51, 235, 344
FatI CATG 2 cut(s) 35, 233
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 222
Hin1II CATG 2 cut(s) 39, 237
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 2 cut(s) 122, 131
HpaI GTTAAC 1 cut(s) 247
HphI GGTGA 2 cut(s) 83, 281
Hpy166II GTNNAC 2 cut(s) 41, 247
Hpy188I TCNGA 2 cut(s) 136, 204
Hpy188III TCNNGA 2 cut(s) 63, 291
Hpy8I GTNNAC 2 cut(s) 41, 247
Hpy99I CGWCG 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 91, 319
HpyCH4V TGCA 4 cut(s) 29, 146, 152, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 4 cut(s) 239, 256, 300, 367
HpySE526I ACGT 2 cut(s) 91, 319
Hsp92II CATG 2 cut(s) 39, 237
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 8 cut(s) 48, 54, 81, 195, 226, 237, 252, 279
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 1 cut(s) 233
MabI ACCWGGT 1 cut(s) 67
MaeII ACGT 2 cut(s) 91, 319
MaeIII GTNAC 1 cut(s) 92
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 2 cut(s) 9, 291
MfeI CAATTG 1 cut(s) 171
MluCI AATT 3 cut(s) 74, 171, 282
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 3 cut(s) 190, 248, 350
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 3 cut(s) 77, 114, 246
MslI CAYNNNNRTG 1 cut(s) 34
MspR9I CCNGG 2 cut(s) 69, 267
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 2 cut(s) 69, 267
MwoI GCNNNNNNNGC 1 cut(s) 370
NdeII GATC 1 cut(s) 376
NlaIII CATG 2 cut(s) 39, 237
NlaIV GGNNCC 1 cut(s) 201
NspI RCATGY 1 cut(s) 39
PciI ACATGT 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 1 cut(s) 122
PflFI GACNNNGTC 1 cut(s) 130
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 65
PscI ACATGT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 67, 265
PspGI CCWGG 2 cut(s) 67, 265
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 65
PspPPI RGGWCCY 1 cut(s) 65
PstNI CAGNNNCTG 1 cut(s) 68
PsyI GACNNNGTC 1 cut(s) 130
RsaI GTAC 2 cut(s) 318, 356
RsaNI GTAC 2 cut(s) 317, 355
RseI CAYNNNNRTG 1 cut(s) 34
SaqAI TTAA 3 cut(s) 77, 114, 246
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 65
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 69, 267
SetI ASST 8 cut(s) 48, 70, 83, 94, 168, 322, 342, 375
SexAI ACCWGGT 1 cut(s) 67
SfaNI GCATC 1 cut(s) 233
SinI GGWCC 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 34
Sse9I AATT 3 cut(s) 74, 171, 282
SsiI CCGC 2 cut(s) 219, 314
StyD4I CCNGG 2 cut(s) 67, 265
TaiI ACGT 2 cut(s) 94, 322
TaqI TCGA 2 cut(s) 125, 195
TasI AATT 3 cut(s) 74, 171, 282
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 3 cut(s) 77, 114, 246
Tru9I TTAA 3 cut(s) 77, 114, 246
TseI GCWGC 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 143
Tth111I GACNNNGTC 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 65
XceI RCATGY 1 cut(s) 39
XmnI GAANNNNTTC 1 cut(s) 356
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.