MD03G1163500.v1.1

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2 ). Among the enzymes to be stimulated by the calmodulin-Ca(2 ) complex are a number of protein kinases and phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017043
Physical Location & Seq
Forward (+)
20365399 .. 20368812
3414 bp
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UTR
Exon/CDS
Intron
MD03G1163500.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGCCGATCAGCTCACCGACGACCAGATCTCTGAGTTCAAGGAGGCTTTCAGCCTATTCGACAAGGACGGCGATGGTTGCATCACTACAAAGGAGTTGGGGACTGTTATGCGTTCACTTGGGCAGAACCCCACTGAAGCTGAGCTTCAGGATATGATCAATGAGGTTGATGCTGATGGGAATGGGACCATTGATTTCCCAGAGTTCCTTAACCTGATGGCCCGGAAGATGAAGGACACAGATTCTGAGGAGGAGCTCAAGGAAGCTTTCCGAGTGTTCGATAAGGACCAGAATGGCTTCATTTCTGCCGCTGAGCTTCGTCATGTTATGACAAATCTAGGCGAGAAGCTGACAGATGAGGAAGTGGATGAGATGATTCGTGAGGCTGATGTGGATGGTGATGGGCAGATCAACTATGAGGAGTTCGTCAAAGTCATGATGGCCAAAATGAGAAGTCGATCAAATCAAAGGAGCGACAGCGTCGGCAGCACGAATTCAAAGGAGTACAAGAACAAGACCGACGGAGGAGAGAACGGGAAGATGGGGCGTCGTTATCGTTGTAGACAACACTTACACGATTGTTCTATCTTGTAA

Protein Analysis

198

Amino Acids

22.37

Weight (kDa)

4.58

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 7 - 76 5.7e-08 Allograft inflammatory factor 1
EF-hand_1 PF00036 12 - 40 5.9e-09 EF hand domain
EF-hand_7 PF13499 12 - 73 3.3e-17 EF-hand domain pair
EF-hand_6 PF13405 12 - 41 4.2e-09 EF-hand domain
EF-hand_9 PF14658 15 - 75 1e-09 EF-hand domain
EF-hand_8 PF13833 25 - 75 1.3e-10 EF-hand domain pair
EF-hand_1 PF00036 48 - 75 5e-08 EF hand domain
EF-hand_FSTL1 PF23564 77 - 152 5.7e-06 Follistatin-related protein 1, EF-hand domain pair
EF-hand_7 PF13499 83 - 147 1.1e-21 EF-hand domain pair
AIF-1 PF21008 84 - 150 2.3e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 85 - 112 3.9e-09 EF hand domain
EF-hand_6 PF13405 85 - 114 3.1e-09 EF-hand domain
EF-hand_5 PF13202 86 - 110 2e-06 EF hand
EF-hand_9 PF14658 88 - 148 4.5e-07 EF-hand domain
EF-hand_8 PF13833 98 - 146 1.6e-12 EF-hand domain pair
EF-hand_1 PF00036 121 - 148 5.7e-10 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 562
AciI CCGC 1 cut(s) 309
AcoI YGGCCR 2 cut(s) 3, 441
AcsI RAATTY 1 cut(s) 493
AcuI CTGAAG 2 cut(s) 131, 156
AcyI GRCGYC 1 cut(s) 547
AfaI GTAC 1 cut(s) 506
AgsI TTSAA 2 cut(s) 40, 498
AluBI AGCT 7 cut(s) 13, 140, 145, 256, 266, 316, 349
AluI AGCT 7 cut(s) 13, 140, 145, 256, 266, 316, 349
Alw21I GWGCWC 1 cut(s) 258
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 3 cut(s) 3, 219, 441
ApeKI GCWGC 1 cut(s) 486
ApoI RAATTY 1 cut(s) 493
Asp700I GAANNNNTTC 1 cut(s) 296
AspS9I GGNCC 3 cut(s) 186, 220, 286
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 7, 410
AvaII GGWCC 2 cut(s) 186, 286
BalI TGGCCA 1 cut(s) 443
BanII GRGCYC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 498
BccI CCATC 7 cut(s) 68, 170, 211, 389, 395, 433, 535
BceAI ACGGC 1 cut(s) 85
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 223
BfaI CTAG 1 cut(s) 338
BglII AGATCT 1 cut(s) 27
BisI GCNGC 2 cut(s) 309, 487
BlpI GCTNAGC 2 cut(s) 141, 312
BlsI GCNGC 2 cut(s) 310, 488
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 2 cut(s) 186, 286
BmgT120I GGNCC 3 cut(s) 186, 220, 286
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 2 cut(s) 90, 160
Bpu1102I GCTNAGC 2 cut(s) 141, 312
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 547
BseGI GGATG 2 cut(s) 373, 400
BseMII CTCAG 4 cut(s) 24, 132, 237, 303
BseRI GAGGAG 4 cut(s) 263, 266, 434, 540
BseXI GCAGC 1 cut(s) 498
BshFI GGCC 3 cut(s) 5, 221, 443
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 2 cut(s) 115, 199
BsmFI GGGAC 2 cut(s) 115, 199
BsnI GGCC 3 cut(s) 5, 221, 443
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 5 cut(s) 7, 27, 156, 408, 458
Bsp1720I GCTNAGC 2 cut(s) 141, 312
BspACI CCGC 1 cut(s) 309
BspANI GGCC 3 cut(s) 5, 221, 443
BspCNI CTCAG 4 cut(s) 25, 133, 238, 304
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 187
BssMI GATC 5 cut(s) 7, 27, 156, 408, 458
BssNI GRCGYC 1 cut(s) 547
Bst4CI ACNGT 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 547
BstDEI CTNAG 4 cut(s) 33, 141, 246, 312
BstF5I GGATG 2 cut(s) 373, 400
BstKTI GATC 5 cut(s) 10, 30, 159, 411, 461
BstMBI GATC 5 cut(s) 7, 27, 156, 408, 458
BstMWI GCNNNNNNNGC 2 cut(s) 78, 486
BstSCI CCNGG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BsuRI GGCC 3 cut(s) 5, 221, 443
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 373, 400
BtsIMutI CAGTG 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 245
CciI TCATGA 1 cut(s) 435
Cfr13I GGNCC 3 cut(s) 186, 220, 286
CseI GACGC 2 cut(s) 469, 536
Csp6I GTAC 1 cut(s) 505
CviAII CATG 2 cut(s) 323, 436
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 4 cut(s) 33, 141, 246, 312
DpnI GATC 5 cut(s) 9, 29, 158, 410, 460
DpnII GATC 5 cut(s) 7, 27, 156, 408, 458
EaeI YGGCCR 2 cut(s) 3, 441
Ecl136II GAGCTC 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 186, 286
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 2 cut(s) 131, 156
EcoICRI GAGCTC 1 cut(s) 256
EcoRI GAATTC 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 258
FaeI CATG 2 cut(s) 326, 439
FaiI YATR 6 cut(s) 110, 155, 324, 329, 417, 437
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 2 cut(s) 115, 199
FatI CATG 2 cut(s) 322, 435
FbaI TGATCA 1 cut(s) 156
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 2 cut(s) 309, 487
FokI GGATG 2 cut(s) 380, 407
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 2 cut(s) 309, 487
FspBI CTAG 1 cut(s) 338
GluI GCNGC 2 cut(s) 309, 487
HaeIII GGCC 3 cut(s) 5, 221, 443
HapII CCGG 1 cut(s) 223
HgaI GACGC 2 cut(s) 469, 536
Hin1I GRCGYC 1 cut(s) 547
Hin1II CATG 2 cut(s) 326, 439
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 2 cut(s) 242, 376
HpaII CCGG 1 cut(s) 223
HphI GGTGA 2 cut(s) 7, 410
Hpy166II GTNNAC 2 cut(s) 116, 563
Hpy188I TCNGA 3 cut(s) 34, 247, 272
Hpy188III TCNNGA 3 cut(s) 149, 380, 436
Hpy8I GTNNAC 2 cut(s) 116, 563
Hpy99I CGWCG 4 cut(s) 23, 485, 524, 552
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 486
HpyF3I CTNAG 4 cut(s) 33, 141, 246, 312
Hsp92I GRCGYC 1 cut(s) 547
Hsp92II CATG 2 cut(s) 326, 439
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 5 cut(s) 7, 27, 156, 408, 458
LmnI GCTCC 2 cut(s) 253, 471
LpnPI CCDG 6 cut(s) 38, 134, 213, 227, 236, 302
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 2 cut(s) 90, 160
MaeI CTAG 1 cut(s) 338
MalI GATC 5 cut(s) 9, 29, 158, 410, 460
MboI GATC 5 cut(s) 7, 27, 156, 408, 458
MboII GAAGA 2 cut(s) 238, 550
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 1 cut(s) 493
MluNI TGGCCA 1 cut(s) 443
MnlI CCTC 8 cut(s) 37, 157, 241, 244, 352, 376, 412, 518
Mox20I TGGCCA 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 443
MseI TTAA 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 1 cut(s) 223
MwoI GCNNNNNNNGC 2 cut(s) 78, 486
NciI CCSGG 1 cut(s) 223
NdeII GATC 5 cut(s) 7, 27, 156, 408, 458
NlaIII CATG 2 cut(s) 326, 439
NlaIV GGNNCC 1 cut(s) 187
PagI TCATGA 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 242, 376
PflFI GACNNNGTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 310, 488
Psp124BI GAGCTC 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 3 cut(s) 186, 220, 286
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 1 cut(s) 27
PsyI GACNNNGTC 1 cut(s) 479
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 2 cut(s) 309, 487
Sau3AI GATC 5 cut(s) 7, 27, 156, 408, 458
Sau96I GGNCC 3 cut(s) 186, 220, 286
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 258
SetI ASST 9 cut(s) 15, 142, 147, 168, 216, 258, 268, 318, 351
SfaNI GCATC 2 cut(s) 90, 160
SinI GGWCC 2 cut(s) 186, 286
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 1 cut(s) 493
SsiI CCGC 1 cut(s) 309
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 3 cut(s) 60, 279, 457
TaqII GACCGA 1 cut(s) 533
TasI AATT 1 cut(s) 493
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 2 cut(s) 242, 376
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 139
TseI GCWGC 1 cut(s) 486
TspDTI ATGAA 2 cut(s) 245, 289
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 1 cut(s) 139
Tth111I GACNNNGTC 1 cut(s) 479
VpaK11BI GGWCC 2 cut(s) 186, 286
XapI RAATTY 1 cut(s) 493
XmiI GTMKAC 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.