Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0017043

Physical Location & Seq

Genomic Coordinates Reverse (-)

17668729 .. 17670746

Gene Structure 2018 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1111300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 450 bp

ATGGCGGATCAACTGACCGACGATCAGATCTCTGAGTTCAAGGAAGCCTTCAGTCTATTCGACAAGGATGGAGATGGCTGTATCACAACTAAGGAGCTTGGCACTGTCATGAGATCATTGGGGCAGAACCCGACTGAGGCAGAGCTCCAGGACATGATCAATGAAGTGGATGCTGATGGTAATGGGACCATTGACTTTCCTGAGTTCTTAAACCTCATGGCCAGGAAGATGAAAGACACCGACTCTGAGGAGGAGCTTAAAGAGGCTTTCAGAGTGTTTGACAAGGATCAGAATGGCTTCATTTCAGCTGCGGAGCTCCGCCATGTGATGACCAATCTTGGGGAGAAGCTCACAGATGAGGAAGTGGATGAGATGATCCGGGAGGCTGATGTTGATGGTGATGGGCAGATAAATTATGAGGAGTTTGTGAAGGTGATGATGGCTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005513 GO:0008150 GO:0009593 GO:0010035 GO:0010038 GO:0010099 GO:0042221 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051592 GO:0051606 GO:0065007 GO:2000026 GO:2000030

KEGG Pathways

Metabolic & Signaling

ko04014 ko04015 ko04016 ko04020 ko04022 ko04024 ko04070 ko04114 ko04218 ko04261 ko04270 ko04371 ko04626 ko04713 ko04720 ko04722 ko04728 ko04740 ko04744 ko04745 ko04750 ko04910 ko04912 ko04915 ko04916 ko04921 ko04922 ko04924 ko04925 ko04970 ko04971 ko05010 ko05031 ko05034 ko05133 ko05152 ko05167 ko05200 ko05214 ko05418 map04014 map04015 map04016 map04020 map04022 map04024 map04070 map04114 map04218 map04261 map04270 map04371 map04626 map04713 map04720 map04722 map04728 map04740 map04744 map04745 map04750 map04910 map04912 map04915 map04916 map04921 map04922 map04924 map04925 map04970 map04971 map05010 map05031 map05034 map05133 map05152 map05167 map05200 map05214 map05418

Pfam Domains

Protein Families

EF-hand_1

Protein Analysis

150

Amino Acids

16.85

Weight (kDa)

4.11

Isoelectric Point (pI)

24.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIF-1	PF21008	7 - 76	2.9e-08	Allograft inflammatory factor 1
EF-hand_1	PF00036	12 - 40	3.8e-09	EF hand domain
EF-hand_7	PF13499	12 - 73	1.8e-17	EF-hand domain pair
EF-hand_6	PF13405	12 - 41	2.7e-09	EF-hand domain
EF-hand_9	PF14658	15 - 75	5.5e-10	EF-hand domain
EF-hand_8	PF13833	25 - 75	7.3e-11	EF-hand domain pair
EF-hand_1	PF00036	48 - 75	3.2e-08	EF hand domain
EF-hand_7	PF13499	83 - 147	5.9e-22	EF-hand domain pair
AIF-1	PF21008	84 - 149	2.8e-06	Allograft inflammatory factor 1
EF-hand_1	PF00036	85 - 112	2.5e-09	EF hand domain
EF-hand_6	PF13405	85 - 114	2e-09	EF-hand domain
EF-hand_5	PF13202	86 - 110	1.3e-06	EF hand
EF-hand_9	PF14658	88 - 148	3.3e-07	EF-hand domain
EF-hand_8	PF13833	98 - 146	1.1e-12	EF-hand domain pair
EF-hand_1	PF00036	121 - 148	3.7e-10	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0017043)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37780
malus_domestica	MD03G1163500.v1.1 MD11G1183900.v1.1 MD12G1111300.v1.1 MD14G1092700.v1.1
prunus_persica	Prupe.4G269100_v2.0.a1 Prupe.4G269100_v2.0.a1 Prupe.4G269100_v2.0.a1 Prupe.7G024100_v2.0.a1
pyrus_communis	pycom12g09360 pycom5153684g00280
rosa_laevigata	RLG00000022641 RLG00000022644

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	5, 311, 319
AclWI	GGATC	3 cut(s)	15, 294, 370
AcoI	YGGCCR	1 cut(s)	219
AcuI	CTGAAG	1 cut(s)	34
AfiI	CCNNNNNNNGG	2 cut(s)	136, 339
AgsI	TTSAA	1 cut(s)	40
AjnI	CCWGG	2 cut(s)	147, 221
AluBI	AGCT	6 cut(s)	97, 145, 256, 308, 316, 349
AluI	AGCT	6 cut(s)	97, 145, 256, 308, 316, 349
Alw21I	GWGCWC	2 cut(s)	147, 318
AlwI	GGATC	3 cut(s)	15, 294, 370
AoxI	GGCC	1 cut(s)	219
ApeKI	GCWGC	1 cut(s)	308
Asp700I	GAANNNNTTC	1 cut(s)	296
AspS9I	GGNCC	1 cut(s)	186
AsuC2I	CCSGG	1 cut(s)	380
AsuHPI	GGTGA	2 cut(s)	410, 445
AvaII	GGWCC	1 cut(s)	186
BalI	TGGCCA	1 cut(s)	221
BanII	GRGCYC	2 cut(s)	147, 318
Bbv12I	GWGCWC	2 cut(s)	147, 318
BbvI	GCAGC	1 cut(s)	295
BccI	CCATC	6 cut(s)	62, 68, 170, 389, 395, 433
BciT130I	CCWGG	2 cut(s)	149, 223
BclI	TGATCA	1 cut(s)	156
BcnI	CCSGG	1 cut(s)	380
BglII	AGATCT	1 cut(s)	27
BisI	GCNGC	1 cut(s)	309
BlsI	GCNGC	1 cut(s)	310
Bme1390I	CCNGG	3 cut(s)	149, 223, 380
Bme18I	GGWCC	1 cut(s)	186
BmgT120I	GGNCC	1 cut(s)	186
BmiI	GGNNCC	1 cut(s)	187
BmrFI	CCNGG	3 cut(s)	149, 223, 380
BmsI	GCATC	1 cut(s)	160
BpmI	CTGGAG	1 cut(s)	131
BpuMI	CCSGG	1 cut(s)	380
Bsc4I	CCNNNNNNNGG	2 cut(s)	136, 339
BseBI	CCWGG	2 cut(s)	149, 223
BseGI	GGATG	3 cut(s)	73, 175, 373
BseLI	CCNNNNNNNGG	2 cut(s)	136, 339
BseMII	CTCAG	4 cut(s)	24, 126, 192, 237
BseRI	GAGGAG	3 cut(s)	263, 266, 434
BseXI	GCAGC	1 cut(s)	295
BshFI	GGCC	1 cut(s)	221
BsiHKAI	GWGCWC	2 cut(s)	147, 318
BsiSI	CCGG	1 cut(s)	379
BslFI	GGGAC	1 cut(s)	199
BslI	CCNNNNNNNGG	2 cut(s)	136, 339
BsmFI	GGGAC	1 cut(s)	199
BsnI	GGCC	1 cut(s)	221
Bsp1286I	GDGCHC	2 cut(s)	147, 318
Bsp143I	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
BspACI	CCGC	3 cut(s)	5, 311, 319
BspANI	GGCC	1 cut(s)	221
BspCNI	CTCAG	4 cut(s)	25, 127, 193, 238
BspHI	TCATGA	1 cut(s)	108
BspLI	GGNNCC	1 cut(s)	187
BspPI	GGATC	3 cut(s)	15, 294, 370
BssMI	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
Bst2UI	CCWGG	2 cut(s)	149, 223
Bst4CI	ACNGT	1 cut(s)	106
BstDEI	CTNAG	6 cut(s)	33, 90, 135, 201, 246, 444
BstF5I	GGATG	3 cut(s)	73, 175, 373
BstKTI	GATC	7 cut(s)	10, 25, 30, 116, 159, 289, 378
BstMBI	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
BstNI	CCWGG	2 cut(s)	149, 223
BstSCI	CCNGG	3 cut(s)	147, 221, 378
BstV1I	GCAGC	1 cut(s)	295
BstX2I	RGATCY	1 cut(s)	27
BstYI	RGATCY	1 cut(s)	27
BsuRI	GGCC	1 cut(s)	221
BtsCI	GGATG	3 cut(s)	73, 175, 373
BtsIMutI	CAGTG	1 cut(s)	102
CciI	TCATGA	1 cut(s)	108
Cfr13I	GGNCC	1 cut(s)	186
CviAII	CATG	4 cut(s)	109, 154, 217, 323
DdeI	CTNAG	6 cut(s)	33, 90, 135, 201, 246, 444
DpnI	GATC	7 cut(s)	9, 24, 29, 115, 158, 288, 377
DpnII	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
EaeI	YGGCCR	1 cut(s)	219
EciI	GGCGGA	2 cut(s)	20, 308
Ecl136II	GAGCTC	2 cut(s)	145, 316
Eco24I	GRGCYC	2 cut(s)	147, 318
Eco47I	GGWCC	1 cut(s)	186
Eco53kI	GAGCTC	2 cut(s)	145, 316
Eco57I	CTGAAG	1 cut(s)	34
EcoICRI	GAGCTC	2 cut(s)	145, 316
EcoRII	CCWGG	2 cut(s)	147, 221
EcoT38I	GRGCYC	2 cut(s)	147, 318
FaeI	CATG	4 cut(s)	112, 157, 220, 326
FaiI	YATR	5 cut(s)	110, 155, 218, 324, 417
FalI	AAGNNNNNCTT	2 cut(s)	32, 64
FaqI	GGGAC	1 cut(s)	199
FatI	CATG	4 cut(s)	108, 153, 216, 322
FbaI	TGATCA	1 cut(s)	156
Fnu4HI	GCNGC	1 cut(s)	309
FokI	GGATG	3 cut(s)	80, 182, 380
FriOI	GRGCYC	2 cut(s)	147, 318
Fsp4HI	GCNGC	1 cut(s)	309
GluI	GCNGC	1 cut(s)	309
GsuI	CTGGAG	1 cut(s)	131
HaeIII	GGCC	1 cut(s)	221
HapII	CCGG	1 cut(s)	379
Hin1II	CATG	4 cut(s)	112, 157, 220, 326
HinfI	GANTC	1 cut(s)	242
HpaII	CCGG	1 cut(s)	379
HphI	GGTGA	2 cut(s)	410, 445
Hpy188I	TCNGA	5 cut(s)	27, 34, 247, 272, 291
Hpy188III	TCNNGA	2 cut(s)	109, 200
Hpy99I	CGWCG	1 cut(s)	23
HpyAV	CCTTC	2 cut(s)	58, 424
HpyCH4III	ACNGT	1 cut(s)	106
HpyF3I	CTNAG	6 cut(s)	33, 90, 135, 201, 246, 444
Hsp92II	CATG	4 cut(s)	112, 157, 220, 326
Ksp22I	TGATCA	1 cut(s)	156
Kzo9I	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
LmnI	GCTCC	5 cut(s)	94, 150, 253, 313, 321
LpnPI	CCDG	6 cut(s)	134, 161, 208, 213, 235, 392
Lsp1109I	GCAGC	1 cut(s)	295
LweI	GCATC	1 cut(s)	160
MalI	GATC	7 cut(s)	9, 24, 29, 115, 158, 288, 377
MboI	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
MboII	GAAGA	1 cut(s)	238
MflI	RGATCY	1 cut(s)	27
MhlI	GDGCHC	2 cut(s)	147, 318
MlsI	TGGCCA	1 cut(s)	221
MluCI	AATT	1 cut(s)	412
MluNI	TGGCCA	1 cut(s)	221
MlyI	GAGTC	1 cut(s)	236
MnlI	CCTC	8 cut(s)	130, 224, 241, 244, 256, 352, 376, 412
Mox20I	TGGCCA	1 cut(s)	221
MroXI	GAANNNNTTC	1 cut(s)	296
MscI	TGGCCA	1 cut(s)	221
MseI	TTAA	2 cut(s)	209, 258
MslI	CAYNNNNRTG	1 cut(s)	107
Msp20I	TGGCCA	1 cut(s)	221
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	1 cut(s)	379
MspR9I	CCNGG	3 cut(s)	149, 223, 380
MvaI	CCWGG	2 cut(s)	149, 223
NciI	CCSGG	1 cut(s)	380
NdeII	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
NlaIII	CATG	4 cut(s)	112, 157, 220, 326
NlaIV	GGNNCC	1 cut(s)	187
PagI	TCATGA	1 cut(s)	108
PdmI	GAANNNNTTC	1 cut(s)	296
PfoI	TCCNGGA	2 cut(s)	147, 378
PkrI	GCNGC	1 cut(s)	310
PleI	GAGTC	1 cut(s)	236
PpsI	GAGTC	1 cut(s)	236
Psp124BI	GAGCTC	2 cut(s)	147, 318
Psp6I	CCWGG	2 cut(s)	147, 221
PspGI	CCWGG	2 cut(s)	147, 221
PspN4I	GGNNCC	1 cut(s)	187
PspPI	GGNCC	1 cut(s)	186
PsuI	RGATCY	1 cut(s)	27
PvuII	CAGCTG	1 cut(s)	308
RseI	CAYNNNNRTG	1 cut(s)	107
SacI	GAGCTC	2 cut(s)	147, 318
SaqAI	TTAA	2 cut(s)	209, 258
SatI	GCNGC	1 cut(s)	309
Sau3AI	GATC	7 cut(s)	7, 22, 27, 113, 156, 286, 375
Sau96I	GGNCC	1 cut(s)	186
SchI	GAGTC	1 cut(s)	236
ScrFI	CCNGG	3 cut(s)	149, 223, 380
SduI	GDGCHC	2 cut(s)	147, 318
SetI	ASST	8 cut(s)	99, 147, 216, 258, 310, 318, 351, 435
SfaNI	GCATC	1 cut(s)	160
SinI	GGWCC	1 cut(s)	186
SmiMI	CAYNNNNRTG	1 cut(s)	107
Sse9I	AATT	1 cut(s)	412
SsiI	CCGC	3 cut(s)	5, 311, 319
SstI	GAGCTC	2 cut(s)	147, 318
StyD4I	CCNGG	3 cut(s)	147, 221, 378
TaaI	ACNGT	1 cut(s)	106
TaqI	TCGA	1 cut(s)	60
TaqII	GACCGA	1 cut(s)	32
TasI	AATT	1 cut(s)	412
Tru1I	TTAA	2 cut(s)	209, 258
Tru9I	TTAA	2 cut(s)	209, 258
TscAI	CASTG	1 cut(s)	109
TseI	GCWGC	1 cut(s)	308
TspDTI	ATGAA	3 cut(s)	177, 245, 289
TspRI	CASTG	1 cut(s)	109
VpaK11BI	GGWCC	1 cut(s)	186
XmnI	GAANNNNTTC	1 cut(s)	296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.