Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013786

Physical Location & Seq

Genomic Coordinates Reverse (-)

21815232 .. 21818341

Gene Structure 3110 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1165100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGGGCAACTGTTGCTCTCAACGCAACACCGAGGATGCCCCCAATGACAACAATAAGGGAGACAACAACAATGCCACAGACAATAACCCTAGCGCTGCCAATTCCGCCCCCAACCCCTCCAGCCACGCTACTCACCCCAATAAACCTTCCGCCTCCCCAACTCACCCTGGAAAGGTCGCCAAGCCGGGCCCTATTGGACCCGTCCTAGGCCGCCCCATGGAGGACGTCCGTACCACGTACAGCATCGGCAAGGAGCTGGGGCGGGGCCAGTTCGGTGTTACCCACTTGTGCACGCACAAGGCCACGGGAGAGCAGTTCGCCTGCAAGACAATTGCCAAGCGGAAGCTGGTGAACAAGGAGGATATTGAGGATGTGAGGAGGGAGGTACAAATCATGCATCATTTGACGGGACAGCAAAACATTGTGGAACTCAAGGGAGCTTATGAGGACAAGCAGTCCGTCCATTTGGTCATGGAGTTGTGCGCCGGAGGTGAGCTTTTCGACAGGATCATCTCAAAAGGACATTACACGGAGCGCGCTGCCGCTGCCTTGTTGAGGACGATAGTTCAGATCGTGCACACGTGTCATACCATGGGCGTTATTCACAGAGATCTCAAGCCTGAGAATTTCCTTTTGCTCAATAAAGATGAAAACTCTCCGCTCAAGGCTACAGATTTTGGTCTATCCGTCTTTTACAAACAAGGAGAAGTATTCAAGGATATTGTGGGCAGTGCATATTACATTGCACCAGAAGTGTTGAAGAGGAGATATGGGCCAGAAGTCGATATTTGGAGTGTTGGAGTCATGTTGTATATTCTTCTCAGTGGTGTTCCTCCCTTCTGGGCAGAATCGGAACATGGAATCTTCAACGCAATCTTACGAGGCCACCTAGACTTTTCAAGCGACCCATGGCCTTCAATATCAACTCAAGCGAAAGATCTTGTCAGGAGGATGTTGAATTCAGACCCCAAACAGAGATTGACAGCCTTCCAAGTTCTTAATCATCCATGGATTAAGGACGATGAAGCACCTGACGTTCCTCTGGACAATGCTGTGCTGAGCCGGCTCAAACAATTTAAAGCAATGAACCAGTTCAAGAAAGTTGCGCTTCGGGTAATTGCGGGATGCTTATCAGAGGAAGAAATCATGGGGCTAAAAGAGATGTTCAAGGGCATTGATACAGACAACAGTGGAACAATAACACTTGAAGAGCTAAGGCAAGGACTTGCTAAGCAAGGGACAAAGCTGTCTGAAGTTGAAGCTCAACAATTAATGGAAGCAGCTGATGCTGATGGCAACGGAACCATAGACTATGATGAGTTCATCACAGCAACGATGCACTTGAACCGGATGGATAAAGAAGAACATCTCTACACTGCCTTCCAACACTTCGATAAAGACAGCAGCGGGTTTATCACGACGGAAGAACTAGAGCAAGTCCTCCGTGAATACGGCATGCATGATGGAAGGGATATCAGGGAAATCATTGCTGAAATTGACGCTGATAATGACGGTCGAATTAATTACGATGAATTTGTAGCAATGATGAGAAAAGGAAACCCAGAAGCCAACCCGAAGAAGCGGCGTGAGGATGTATTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 Pkinase

Protein Analysis

535

Amino Acids

59.75

Weight (kDa)

5.67

Isoelectric Point (pI)

40.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	80 - 338	5.5e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	82 - 332	1.9e-27	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	385 - 411	6.4e-07	EF hand domain
EF-hand_7	PF13499	385 - 445	3.2e-14	EF-hand domain pair
EF-hand_6	PF13405	385 - 411	4e-06	EF-hand domain
EF-hand_7	PF13499	454 - 517	4.1e-17	EF-hand domain pair
EF-hand_6	PF13405	457 - 485	1.4e-06	EF-hand domain
EF-hand_1	PF00036	459 - 483	9e-06	EF hand domain
EF-hand_1	PF00036	491 - 518	1.8e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12180 AT5G19360
fragaria_vesca	FvH4_3g27800
malus_domestica	MD03G1165100.v1.1 MD11G1184100.v1.1
prunus_persica	Prupe.4G263600_v2.0.a1
pyrus_communis	pycom12g08860
rosa_chinensis	RchiOBHm_Chr5g0050441
rosa_laevigata	RLG00000034702
rosa_multiflora	Rmu_co8347277.1_g000001 Rmu_sc0003202.1_g000003
rosa_roxburghii	Rroxscaffold_1G00030430
rosa_rugosa	Rorug05G0261000
rosa_samantha	Rh5AG332700 Rh5CG370000 Rh5DG355500
rosa_wichuraiana	Rw5G031440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1246
AatII	GACGTC	1 cut(s)	228
AccBSI	CCGCTC	1 cut(s)	661
AccII	CGCG	1 cut(s)	537
AclWI	GGATC	1 cut(s)	515
AcsI	RAATTY	3 cut(s)	625, 958, 1532
AcuI	CTGAAG	1 cut(s)	1272
AcvI	CACGTG	1 cut(s)	582
AcyI	GRCGYC	1 cut(s)	225
AfaI	GTAC	3 cut(s)	232, 239, 387
AfeI	AGCGCT	1 cut(s)	94
AfiI	CCNNNNNNNGG	5 cut(s)	172, 206, 217, 220, 555
AflIII	ACRYGT	2 cut(s)	579, 581
AhdI	GACNNNNNGTC	1 cut(s)	454
AjnI	CCWGG	1 cut(s)	166
AluBI	AGCT	8 cut(s)	256, 346, 440, 496, 1213, 1246, 1262, 1283
AluI	AGCT	8 cut(s)	256, 346, 440, 496, 1213, 1246, 1262, 1283
Alw21I	GWGCWC	2 cut(s)	293, 579
Alw26I	GTCTC	1 cut(s)	54
Alw44I	GTGCAC	2 cut(s)	289, 575
AlwI	GGATC	1 cut(s)	515
Aor51HI	AGCGCT	1 cut(s)	94
AoxI	GGCC	7 cut(s)	187, 208, 265, 300, 773, 883, 911
ApaI	GGGCCC	1 cut(s)	191
ApaLI	GTGCAC	2 cut(s)	289, 575
ApeKI	GCWGC	5 cut(s)	95, 539, 545, 1280, 1404
ApoI	RAATTY	3 cut(s)	625, 958, 1532
AseI	ATTAAT	2 cut(s)	1271, 1521
Asp700I	GAANNNNTTC	1 cut(s)	1091
AspA2I	CCTAGG	1 cut(s)	205
AspLEI	GCGC	5 cut(s)	95, 485, 537, 539, 1108
AspS9I	GGNCC	5 cut(s)	187, 188, 197, 265, 773
AsuC2I	CCSGG	1 cut(s)	186
AsuHPI	GGTGA	4 cut(s)	125, 155, 361, 503
AvaII	GGWCC	1 cut(s)	197
AvrII	CCTAGG	1 cut(s)	205
BaeGI	GKGCMC	3 cut(s)	191, 293, 579
BaeI	ACNNNNGTAYC	2 cut(s)	214, 247
BanII	GRGCYC	1 cut(s)	191
BbrPI	CACGTG	1 cut(s)	582
Bbv12I	GWGCWC	2 cut(s)	293, 579
BbvI	GCAGC	5 cut(s)	82, 526, 532, 1292, 1416
BccI	CCATC	3 cut(s)	1286, 1345, 1457
BceAI	ACGGC	1 cut(s)	1468
BciT130I	CCWGG	1 cut(s)	168
BcnI	CCSGG	1 cut(s)	186
BcoDI	GTCTC	1 cut(s)	54
BfaI	CTAG	4 cut(s)	90, 206, 890, 1430
BfmI	CTRYAG	1 cut(s)	669
BfoI	RGCGCY	1 cut(s)	96
BglII	AGATCT	2 cut(s)	610, 937
BisI	GCNGC	8 cut(s)	96, 211, 540, 543, 546, 1281, 1405, 1583
BlnI	CCTAGG	1 cut(s)	205
BlpI	GCTNAGC	2 cut(s)	1058, 1230
BlsI	GCNGC	8 cut(s)	97, 212, 541, 544, 547, 1282, 1406, 1584
Bme1390I	CCNGG	2 cut(s)	168, 186
Bme18I	GGWCC	1 cut(s)	197
BmeRI	GACNNNNNGTC	1 cut(s)	454
BmgT120I	GGNCC	5 cut(s)	187, 188, 197, 265, 773
BmiI	GGNNCC	4 cut(s)	189, 199, 266, 1303
BmrFI	CCNGG	2 cut(s)	168, 186
BmsI	GCATC	6 cut(s)	25, 252, 406, 1115, 1276, 1326
BpmI	CTGGAG	1 cut(s)	103
Bpu10I	CCTNAGC	1 cut(s)	1214
Bpu1102I	GCTNAGC	2 cut(s)	1058, 1230
BpuEI	CTTGAG	4 cut(s)	416, 599, 647, 912
BpuMI	CCSGG	1 cut(s)	186
BsaAI	YACGTR	2 cut(s)	237, 582
BsaBI	GATNNNNATC	1 cut(s)	1129
BsaHI	GRCGYC	1 cut(s)	225
BsaJI	CCNNGG	8 cut(s)	30, 166, 205, 216, 303, 591, 908, 1007
BsaWI	WCCGGW	1 cut(s)	1347
Bsc4I	CCNNNNNNNGG	5 cut(s)	172, 206, 217, 220, 555
Bse118I	RCCGGY	1 cut(s)	1062
Bse1I	ACTGG	2 cut(s)	268, 1090
Bse3DI	GCAATG	4 cut(s)	741, 1089, 1485, 1548
Bse8I	GATNNNNATC	1 cut(s)	1129
BseBI	CCWGG	1 cut(s)	168
BseDI	CCNNGG	8 cut(s)	30, 166, 205, 216, 303, 591, 908, 1007
BseGI	GGATG	7 cut(s)	40, 376, 957, 1003, 1130, 1356, 1597
BseJI	GATNNNNATC	1 cut(s)	1129
BseLI	CCNNNNNNNGG	5 cut(s)	172, 206, 217, 220, 555
BseMI	GCAATG	4 cut(s)	741, 1089, 1485, 1548
BseMII	CTCAG	3 cut(s)	612, 835, 1049
BseNI	ACTGG	2 cut(s)	268, 1090
BsePI	GCGCGC	1 cut(s)	535
BseRI	GAGGAG	2 cut(s)	391, 778
BseSI	GKGCMC	3 cut(s)	191, 293, 579
BseXI	GCAGC	5 cut(s)	82, 526, 532, 1292, 1416
BseYI	CCCAGC	1 cut(s)	256
Bsh1236I	CGCG	1 cut(s)	537
Bsh1285I	CGRYCG	1 cut(s)	1516
BshFI	GGCC	7 cut(s)	189, 210, 267, 302, 775, 885, 913
BsiEI	CGRYCG	1 cut(s)	1516
BsiHKAI	GWGCWC	2 cut(s)	293, 579
BsiSI	CCGG	4 cut(s)	185, 486, 1063, 1348
BslFI	GGGAC	2 cut(s)	423, 1252
BslI	CCNNNNNNNGG	5 cut(s)	172, 206, 217, 220, 555
BsmAI	GTCTC	1 cut(s)	54
BsmFI	GGGAC	2 cut(s)	423, 1252
BsnI	GGCC	7 cut(s)	189, 210, 267, 302, 775, 885, 913
Bsp120I	GGGCCC	1 cut(s)	187
Bsp1286I	GDGCHC	3 cut(s)	191, 293, 579
Bsp143I	GATC	4 cut(s)	507, 570, 610, 937
Bsp1720I	GCTNAGC	2 cut(s)	1058, 1230
Bsp19I	CCATGG	4 cut(s)	216, 591, 908, 1007
BspANI	GGCC	7 cut(s)	189, 210, 267, 302, 775, 885, 913
BspCNI	CTCAG	3 cut(s)	613, 834, 1050
BspFNI	CGCG	1 cut(s)	537
BspLI	GGNNCC	4 cut(s)	189, 199, 266, 1303
BspPI	GGATC	1 cut(s)	515
BspQI	GCTCTTC	1 cut(s)	1203
BsrBI	CCGCTC	1 cut(s)	661
BsrDI	GCAATG	4 cut(s)	741, 1089, 1485, 1548
BsrFI	RCCGGY	1 cut(s)	1062
BsrI	ACTGG	2 cut(s)	268, 1090
BssAI	RCCGGY	1 cut(s)	1062
BssECI	CCNNGG	8 cut(s)	30, 166, 205, 216, 303, 591, 908, 1007
BssHII	GCGCGC	1 cut(s)	535
BssMI	GATC	4 cut(s)	507, 570, 610, 937
BssNI	GRCGYC	1 cut(s)	225
BssT1I	CCWWGG	5 cut(s)	205, 216, 591, 908, 1007
Bst2UI	CCWGG	1 cut(s)	168
Bst4CI	ACNGT	3 cut(s)	11, 1190, 1514
Bst6I	CTCTTC	2 cut(s)	755, 1203
BstACI	GRCGYC	1 cut(s)	225
BstAPI	GCANNNNNTGC	2 cut(s)	12, 1286
BstBAI	YACGTR	2 cut(s)	237, 582
BstC8I	GCNNGC	5 cut(s)	293, 322, 537, 1064, 1457
BstDEI	CTNAG	5 cut(s)	621, 821, 1058, 1214, 1230
BstDSI	CCRYGG	5 cut(s)	216, 303, 591, 908, 1007
BstENI	CCTNNNNNAGG	1 cut(s)	553
BstF5I	GGATG	7 cut(s)	40, 376, 957, 1003, 1130, 1356, 1597
BstFNI	CGCG	1 cut(s)	537
BstH2I	RGCGCY	1 cut(s)	96
BstHHI	GCGC	5 cut(s)	95, 485, 537, 539, 1108
BstKTI	GATC	4 cut(s)	510, 573, 613, 940
BstMAI	GTCTC	1 cut(s)	54
BstMBI	GATC	4 cut(s)	507, 570, 610, 937
BstMCI	CGRYCG	1 cut(s)	1516
BstMWI	GCNNNNNNNGC	6 cut(s)	12, 21, 104, 545, 1063, 1286
BstNI	CCWGG	1 cut(s)	168
BstNSI	RCATGY	1 cut(s)	1459
BstSCI	CCNGG	2 cut(s)	166, 184
BstSFI	CTRYAG	1 cut(s)	669
BstSLI	GKGCMC	3 cut(s)	191, 293, 579
BstUI	CGCG	1 cut(s)	537
BstV1I	GCAGC	5 cut(s)	82, 526, 532, 1292, 1416
BstX2I	RGATCY	2 cut(s)	610, 937
BstYI	RGATCY	2 cut(s)	610, 937
BsuRI	GGCC	7 cut(s)	189, 210, 267, 302, 775, 885, 913
BtgI	CCRYGG	5 cut(s)	216, 303, 591, 908, 1007
BtsCI	GGATG	7 cut(s)	40, 376, 957, 1003, 1130, 1356, 1597
BtsI	GCAGTG	2 cut(s)	736, 1374
BtsIMutI	CAGTG	4 cut(s)	736, 829, 1195, 1374
Cac8I	GCNNGC	5 cut(s)	293, 322, 537, 1064, 1457
CfoI	GCGC	5 cut(s)	95, 485, 537, 539, 1108
Cfr10I	RCCGGY	1 cut(s)	1062
Cfr13I	GGNCC	5 cut(s)	187, 188, 197, 265, 773
CseI	GACGC	1 cut(s)	1508
Csp6I	GTAC	3 cut(s)	231, 238, 386
CviQI	GTAC	3 cut(s)	231, 238, 386
DdeI	CTNAG	5 cut(s)	621, 821, 1058, 1214, 1230
DpnI	GATC	4 cut(s)	509, 572, 612, 939
DpnII	GATC	4 cut(s)	507, 570, 610, 937
DraI	TTTAAA	1 cut(s)	1078
DrdI	GACNNNNNNGTC	1 cut(s)	1246
DriI	GACNNNNNGTC	1 cut(s)	454
DseDI	GACNNNNNNGTC	1 cut(s)	1246
Eam1104I	CTCTTC	2 cut(s)	755, 1203
Eam1105I	GACNNNNNGTC	1 cut(s)	454
EarI	CTCTTC	2 cut(s)	755, 1203
EciI	GGCGGA	2 cut(s)	94, 139
Eco130I	CCWWGG	5 cut(s)	205, 216, 591, 908, 1007
Eco24I	GRGCYC	1 cut(s)	191
Eco32I	GATATC	1 cut(s)	1474
Eco47I	GGWCC	1 cut(s)	197
Eco47III	AGCGCT	1 cut(s)	94
Eco57I	CTGAAG	1 cut(s)	1272
Eco72I	CACGTG	1 cut(s)	582
EcoNI	CCTNNNNNAGG	1 cut(s)	553
EcoO109I	RGGNCCY	1 cut(s)	188
EcoRI	GAATTC	1 cut(s)	958
EcoRII	CCWGG	1 cut(s)	166
EcoRV	GATATC	1 cut(s)	1474
EcoT14I	CCWWGG	5 cut(s)	205, 216, 591, 908, 1007
EcoT22I	ATGCAT	2 cut(s)	399, 1461
EcoT38I	GRGCYC	1 cut(s)	191
ErhI	CCWWGG	5 cut(s)	205, 216, 591, 908, 1007
FalI	AAGNNNNNCTT	2 cut(s)	1092, 1124
FaqI	GGGAC	2 cut(s)	423, 1252
FauI	CCCGC	3 cut(s)	255, 1114, 1400
Fnu4HI	GCNGC	8 cut(s)	96, 211, 540, 543, 546, 1281, 1405, 1583
FokI	GGATG	6 cut(s)	47, 383, 964, 990, 1137, 1363
FriOI	GRGCYC	1 cut(s)	191
Fsp4HI	GCNGC	8 cut(s)	96, 211, 540, 543, 546, 1281, 1405, 1583
FspBI	CTAG	4 cut(s)	90, 206, 890, 1430
GlaI	GCGC	5 cut(s)	94, 484, 536, 538, 1107
GluI	GCNGC	8 cut(s)	96, 211, 540, 543, 546, 1281, 1405, 1583
GsaI	CCCAGC	1 cut(s)	260
GsuI	CTGGAG	1 cut(s)	103
HaeII	RGCGCY	1 cut(s)	96
HaeIII	GGCC	7 cut(s)	189, 210, 267, 302, 775, 885, 913
HapII	CCGG	4 cut(s)	185, 486, 1063, 1348
HgaI	GACGC	1 cut(s)	1508
HhaI	GCGC	5 cut(s)	95, 485, 537, 539, 1108
Hin1I	GRCGYC	1 cut(s)	225
Hin6I	GCGC	5 cut(s)	93, 483, 535, 537, 1106
HinP1I	GCGC	5 cut(s)	93, 483, 535, 537, 1106
HinfI	GANTC	3 cut(s)	801, 848, 861
HpaII	CCGG	4 cut(s)	185, 486, 1063, 1348
HphI	GGTGA	4 cut(s)	125, 155, 361, 503
Hpy166II	GTNNAC	3 cut(s)	291, 352, 577
Hpy188I	TCNGA	5 cut(s)	570, 853, 964, 1135, 1252
Hpy188III	TCNNGA	4 cut(s)	946, 1043, 1096, 1417
Hpy8I	GTNNAC	3 cut(s)	291, 352, 577
Hpy99I	CGWCG	1 cut(s)	1423
HpyAV	CCTTC	6 cut(s)	156, 847, 924, 997, 1390, 1461
HpyCH4III	ACNGT	3 cut(s)	11, 1190, 1514
HpyCH4IV	ACGT	4 cut(s)	225, 236, 581, 1035
HpyCH4V	TGCA	8 cut(s)	291, 324, 397, 577, 734, 746, 1339, 1459
HpyF10VI	GCNNNNNNNGC	6 cut(s)	12, 21, 104, 545, 1063, 1286
HpyF3I	CTNAG	5 cut(s)	621, 821, 1058, 1214, 1230
HpySE526I	ACGT	4 cut(s)	225, 236, 581, 1035
Hsp92I	GRCGYC	1 cut(s)	225
HspAI	GCGC	5 cut(s)	93, 483, 535, 537, 1106
KroI	GCCGGC	1 cut(s)	1062
KroNI	GCCGGC	1 cut(s)	1064
Kzo9I	GATC	4 cut(s)	507, 570, 610, 937
LguI	GCTCTTC	1 cut(s)	1203
LmnI	GCTCC	3 cut(s)	253, 437, 532
Lsp1109I	GCAGC	5 cut(s)	82, 526, 532, 1292, 1416
LweI	GCATC	6 cut(s)	25, 252, 406, 1115, 1276, 1326
MaeI	CTAG	4 cut(s)	90, 206, 890, 1430
MaeII	ACGT	4 cut(s)	225, 236, 581, 1035
MaeIII	GTNAC	1 cut(s)	277
MalI	GATC	4 cut(s)	509, 572, 612, 939
MbiI	CCGCTC	1 cut(s)	661
MboI	GATC	4 cut(s)	507, 570, 610, 937
MboII	GAAGA	8 cut(s)	772, 809, 856, 1151, 1220, 1373, 1436, 1588
MfeI	CAATTG	1 cut(s)	330
MflI	RGATCY	2 cut(s)	610, 937
MhlI	GDGCHC	3 cut(s)	191, 293, 579
MlyI	GAGTC	1 cut(s)	810
MmeI	TCCRAC	2 cut(s)	778, 1408
Mph1103I	ATGCAT	2 cut(s)	399, 1461
MroNI	GCCGGC	1 cut(s)	1062
MroXI	GAANNNNTTC	1 cut(s)	1091
MseI	TTAA	5 cut(s)	999, 1014, 1077, 1271, 1521
MspA1I	CMGCKG	3 cut(s)	545, 1283, 1407
MspI	CCGG	4 cut(s)	185, 486, 1063, 1348
MspR9I	CCNGG	2 cut(s)	168, 186
MunI	CAATTG	1 cut(s)	330
MvaI	CCWGG	1 cut(s)	168
MvnI	CGCG	1 cut(s)	537
MwoI	GCNNNNNNNGC	6 cut(s)	12, 21, 104, 545, 1063, 1286
NaeI	GCCGGC	1 cut(s)	1064
NciI	CCSGG	1 cut(s)	186
NcoI	CCATGG	4 cut(s)	216, 591, 908, 1007
NdeII	GATC	4 cut(s)	507, 570, 610, 937
NgoMIV	GCCGGC	1 cut(s)	1062
NlaIV	GGNNCC	4 cut(s)	189, 199, 266, 1303
NsiI	ATGCAT	2 cut(s)	399, 1461
NspI	RCATGY	1 cut(s)	1459
PaeI	GCATGC	1 cut(s)	1459
PauI	GCGCGC	1 cut(s)	535
PciSI	GCTCTTC	1 cut(s)	1203
PdiI	GCCGGC	1 cut(s)	1064
PdmI	GAANNNNTTC	1 cut(s)	1091
PfeI	GAWTC	2 cut(s)	848, 861
PkrI	GCNGC	8 cut(s)	97, 212, 541, 544, 547, 1282, 1406, 1584
PleI	GAGTC	1 cut(s)	809
PmaCI	CACGTG	1 cut(s)	582
PmlI	CACGTG	1 cut(s)	582
PpsI	GAGTC	1 cut(s)	809
Ppu21I	YACGTR	2 cut(s)	237, 582
PshBI	ATTAAT	2 cut(s)	1271, 1521
Psp6I	CCWGG	1 cut(s)	166
PspCI	CACGTG	1 cut(s)	582
PspFI	CCCAGC	1 cut(s)	256
PspGI	CCWGG	1 cut(s)	166
PspN4I	GGNNCC	4 cut(s)	189, 199, 266, 1303
PspOMI	GGGCCC	1 cut(s)	187
PspPI	GGNCC	5 cut(s)	187, 188, 197, 265, 773
PsuI	RGATCY	2 cut(s)	610, 937
PteI	GCGCGC	1 cut(s)	535
PvuII	CAGCTG	1 cut(s)	1283
RsaI	GTAC	3 cut(s)	232, 239, 387
RsaNI	GTAC	3 cut(s)	231, 238, 386
SapI	GCTCTTC	1 cut(s)	1203
SaqAI	TTAA	5 cut(s)	999, 1014, 1077, 1271, 1521
SatI	GCNGC	8 cut(s)	96, 211, 540, 543, 546, 1281, 1405, 1583
Sau3AI	GATC	4 cut(s)	507, 570, 610, 937
Sau96I	GGNCC	5 cut(s)	187, 188, 197, 265, 773
SchI	GAGTC	1 cut(s)	810
ScrFI	CCNGG	2 cut(s)	168, 186
SduI	GDGCHC	3 cut(s)	191, 293, 579
SfaNI	GCATC	6 cut(s)	25, 252, 406, 1115, 1276, 1326
SfcI	CTRYAG	1 cut(s)	669
SinI	GGWCC	1 cut(s)	197
SmlI	CTYRAG	4 cut(s)	431, 614, 662, 927
SmoI	CTYRAG	4 cut(s)	431, 614, 662, 927
SphI	GCATGC	1 cut(s)	1459
SspMI	CTAG	4 cut(s)	90, 206, 890, 1430
StyD4I	CCNGG	2 cut(s)	166, 184
StyI	CCWWGG	5 cut(s)	205, 216, 591, 908, 1007
TaaI	ACNGT	3 cut(s)	11, 1190, 1514
TaiI	ACGT	4 cut(s)	228, 239, 584, 1038
TaqI	TCGA	4 cut(s)	501, 783, 1392, 1516
TauI	GCSGC	3 cut(s)	213, 545, 1585
TfiI	GAWTC	2 cut(s)	848, 861
Tru1I	TTAA	5 cut(s)	999, 1014, 1077, 1271, 1521
Tru9I	TTAA	5 cut(s)	999, 1014, 1077, 1271, 1521
TscAI	CASTG	4 cut(s)	736, 829, 1195, 1381
TseI	GCWGC	5 cut(s)	95, 539, 545, 1280, 1404
TspDTI	ATGAA	5 cut(s)	663, 1038, 1100, 1312, 1545
TspGWI	ACGGA	7 cut(s)	218, 448, 545, 676, 1314, 1433, 1436
TspRI	CASTG	4 cut(s)	736, 829, 1195, 1381
VneI	GTGCAC	2 cut(s)	289, 575
VpaK11BI	GGWCC	1 cut(s)	197
VspI	ATTAAT	2 cut(s)	1271, 1521
XagI	CCTNNNNNAGG	1 cut(s)	553
XapI	RAATTY	3 cut(s)	625, 958, 1532
XceI	RCATGY	1 cut(s)	1459
XcmI	CCANNNNNNNNNTGG	1 cut(s)	343
XmaJI	CCTAGG	1 cut(s)	205
XmnI	GAANNNNTTC	1 cut(s)	1091
XspI	CTAG	4 cut(s)	90, 206, 890, 1430
ZraI	GACGTC	1 cut(s)	226
Zsp2I	ATGCAT	2 cut(s)	399, 1461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.