Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0013786

Physical Location & Seq

Genomic Coordinates Reverse (-)

24506611 .. 24509253

Gene Structure 2643 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1184100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGGGCAACTGTTGCTCTCAATGCAACACCGAGGATGCCCCAAACGACGACAATAAGGGTGACAACAACTATGCCGCGGACAAAAACCCTACGGCTGCCAATTCTGCCCCCAACCCCAGCCACGCTAATCACCATAAACCTTCCGCCTCCCCAACTCACCCAGGAAAGGCCGCCAAACCGGGCCCTATTGGCCCCGTCCTAGGCCGCCCCATGGAGGACGTCCACACCACGTACAGCATTGGCAAGGAGCTGGGACGGGGCCAGTTCGGTGTTACCCACTTGTGCACGCACAAGGCCACGGGAGAGCAGTTCGCCTGCAAGACAATCGCCAAGCGGAAGCTGGTGAACAAGGAAGATATTGAGGATGTGAGGAGGGAGGTACAGATCATGCATCATTTGACGGGGCAGCCAAACATTGTGGAACTCAAGGGAGCTTATGAGGACAAGCAGTCGGTTCATTTGGTCATGGAGTTGTGCGCAGGAGGAGAGCTTTTCGACAGGATCATCTCAAAAGGACATTACACGGAGCGCGCTGCCGCTGCCTTGTTGAGGACGATTGTTCAGATCGTGCACACGTGTCATTCCATGGGCGTTATTCACAGAGATCTCAAGCCTGAGAATTTCCTTATGCTCAATAAAGATGAAAACTCTCCGGTCAAGGTTACAGATTTTGGTCTATCCGTCTTTTACAAACAAGGAGAAGTATTCAAGGATATTGTGGGGACTACATATTACATTGCACCAGAAGTGTTGAAGAGGAGATATGGGCCAGAAGTCGATATTTGGAGTGTTGGTGTCATGTTGTATATACTTCTCAGTGGTGTTCCTCCCTTCTGGGCAGAATCCGAATATGGGATCTTCAATGCCATCTCACGAGGCCACCTCAACTTTTCAAGCGACCCATGGCCTTCAATATCAACTCAAGCGAAAGATCTTGTAAGGAAGATGTTGAATTCAGACCCCAAACAGAGATTGACAGCCTTCCAAGTTCTTAATCATCCATGGATTAAGGAGGATGGCGAAGCACCTGACATTCCTCTGGACAACGCTGTGCTCAGCAGGCTTAAACAATTTAAAGCAATGAACGAGTTCAAGAAAGTTGCGCTTCGGGTAATTGCGGGTTGCTTATCAGAGGAAGAAATCATAGGGCTAAAAGAGATGTTCAAGGGCATAGATACAGATAATAGTGGAACAATAACACTTGAAGAGCTGAAGCAAGGACTTGCTAAGCAAGGGACAAAGCTGTCTGAACTTGAAGCTCAACAATTAATGGAAGCAGCTGATGCTGATCGCAACGGAACCATAGACTATGATGAGTTCATCACAGCAACAATGCACCTGAACCGGATGGATAGAGAAGAACATCTCTACACTGCCTTCCAACACTTCGATGCCGACAACAGCGGGTATATCACGACGGCAGAACTAGAGCAAGCCCTCCGCGATTACGGCATGCATGATGGAAGGGATATCAGAGAAATCATTGCTGAAATTGACGCTGATAATGACGGTCGAATTAATTACAATGAATTTGTGGCAATGATGAGAAAAGGAAATCCAGAAGCCAACCCGAAGAAGCGGCGTGACGATGTATTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 Pkinase

Protein Analysis

534

Amino Acids

59.61

Weight (kDa)

5.7

Isoelectric Point (pI)

36.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	78 - 336	2.1e-79	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	80 - 330	6.1e-30	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	384 - 410	6.1e-07	EF hand domain
EF-hand_7	PF13499	384 - 444	1.4e-13	EF-hand domain pair
EF-hand_6	PF13405	384 - 410	4.5e-06	EF-hand domain
EF-hand_7	PF13499	453 - 516	2.4e-17	EF-hand domain pair
EF-hand_6	PF13405	456 - 484	1.4e-06	EF-hand domain
EF-hand_1	PF00036	490 - 517	3e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12180 AT5G19360
fragaria_vesca	FvH4_3g27800
malus_domestica	MD03G1165100.v1.1 MD11G1184100.v1.1
prunus_persica	Prupe.4G263600_v2.0.a1
pyrus_communis	pycom12g08860
rosa_chinensis	RchiOBHm_Chr5g0050441
rosa_laevigata	RLG00000034702
rosa_multiflora	Rmu_co8347277.1_g000001 Rmu_sc0003202.1_g000003
rosa_roxburghii	Rroxscaffold_1G00030430
rosa_rugosa	Rorug05G0261000
rosa_samantha	Rh5AG332700 Rh5CG370000 Rh5DG355500
rosa_wichuraiana	Rw5G031440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1243
AatII	GACGTC	1 cut(s)	222
Acc16I	TGCGCA	1 cut(s)	478
AccII	CGCG	3 cut(s)	77, 531, 1443
AclWI	GGATC	2 cut(s)	509, 863
AcsI	RAATTY	3 cut(s)	619, 952, 1529
AcuI	CTGAAG	1 cut(s)	1232
AcvI	CACGTG	1 cut(s)	576
AcyI	GRCGYC	1 cut(s)	219
AfaI	GTAC	2 cut(s)	233, 381
AfiI	CCNNNNNNNGG	6 cut(s)	166, 200, 211, 214, 549, 852
AflIII	ACRYGT	2 cut(s)	573, 575
AhdI	GACNNNNNGTC	1 cut(s)	448
AjnI	CCWGG	1 cut(s)	160
AluBI	AGCT	8 cut(s)	250, 340, 434, 490, 1210, 1243, 1259, 1280
AluI	AGCT	8 cut(s)	250, 340, 434, 490, 1210, 1243, 1259, 1280
Alw21I	GWGCWC	3 cut(s)	287, 573, 1056
Alw44I	GTGCAC	2 cut(s)	283, 569
AlwI	GGATC	2 cut(s)	509, 863
AoxI	GGCC	9 cut(s)	168, 181, 190, 202, 259, 294, 767, 877, 905
ApaI	GGGCCC	1 cut(s)	185
ApaLI	GTGCAC	2 cut(s)	283, 569
ApeKI	GCWGC	5 cut(s)	95, 406, 533, 539, 1277
ApoI	RAATTY	3 cut(s)	619, 952, 1529
ArsI	GACNNNNNNTTYG	1 cut(s)	1577
AseI	ATTAAT	2 cut(s)	1268, 1518
Asp700I	GAANNNNTTC	1 cut(s)	1088
AspA2I	CCTAGG	1 cut(s)	199
AspLEI	GCGC	4 cut(s)	479, 531, 533, 1105
AspS9I	GGNCC	5 cut(s)	181, 182, 191, 259, 767
AsuC2I	CCSGG	1 cut(s)	180
AsuHPI	GGTGA	4 cut(s)	71, 122, 149, 355
AvrII	CCTAGG	1 cut(s)	199
BaeGI	GKGCMC	3 cut(s)	185, 287, 573
BanII	GRGCYC	1 cut(s)	185
BauI	CACGAG	1 cut(s)	873
BbrPI	CACGTG	1 cut(s)	576
Bbv12I	GWGCWC	3 cut(s)	287, 573, 1056
BbvI	GCAGC	5 cut(s)	82, 418, 520, 526, 1289
BccI	CCATC	4 cut(s)	875, 1010, 1342, 1454
BceAI	ACGGC	3 cut(s)	108, 1434, 1465
BcgI	CGANNNNNNTGC	2 cut(s)	307, 341
BciT130I	CCWGG	1 cut(s)	162
BcnI	CCSGG	1 cut(s)	180
BfaI	CTAG	2 cut(s)	200, 1427
BglI	GCCNNNNNGGC	1 cut(s)	189
BglII	AGATCT	2 cut(s)	604, 931
BlnI	CCTAGG	1 cut(s)	199
BlpI	GCTNAGC	2 cut(s)	1055, 1227
Bme1390I	CCNGG	2 cut(s)	162, 180
BmeRI	GACNNNNNGTC	1 cut(s)	448
BmgT120I	GGNCC	5 cut(s)	181, 182, 191, 259, 767
BmiI	GGNNCC	4 cut(s)	183, 193, 260, 1300
BmrFI	CCNGG	2 cut(s)	162, 180
BmsI	GCATC	4 cut(s)	25, 400, 1273, 1381
BplI	GAGNNNNNCTC	2 cut(s)	867, 899
Bpu1102I	GCTNAGC	2 cut(s)	1055, 1227
BpuEI	CTTGAG	3 cut(s)	410, 593, 906
BpuMI	CCSGG	1 cut(s)	180
BsaAI	YACGTR	2 cut(s)	231, 576
BsaBI	GATNNNNATC	1 cut(s)	1287
BsaHI	GRCGYC	1 cut(s)	219
BsaJI	CCNNGG	9 cut(s)	30, 75, 160, 199, 210, 297, 585, 902, 1001
BsaWI	WCCGGW	2 cut(s)	652, 1344
BsaXI	ACNNNNNCTCC	2 cut(s)	778, 808
Bsc4I	CCNNNNNNNGG	6 cut(s)	166, 200, 211, 214, 549, 852
Bse1I	ACTGG	1 cut(s)	262
Bse3DI	GCAATG	4 cut(s)	735, 1086, 1482, 1545
Bse8I	GATNNNNATC	1 cut(s)	1287
BseBI	CCWGG	1 cut(s)	162
BseDI	CCNNGG	9 cut(s)	30, 75, 160, 199, 210, 297, 585, 902, 1001
BseGI	GGATG	5 cut(s)	40, 370, 997, 1021, 1353
BseJI	GATNNNNATC	1 cut(s)	1287
BseLI	CCNNNNNNNGG	6 cut(s)	166, 200, 211, 214, 549, 852
BseMI	GCAATG	4 cut(s)	735, 1086, 1482, 1545
BseMII	CTCAG	3 cut(s)	606, 829, 1069
BseNI	ACTGG	1 cut(s)	262
BsePI	GCGCGC	1 cut(s)	529
BseRI	GAGGAG	3 cut(s)	385, 498, 772
BseSI	GKGCMC	3 cut(s)	185, 287, 573
BseXI	GCAGC	5 cut(s)	82, 418, 520, 526, 1289
BseYI	CCCAGC	2 cut(s)	116, 250
Bsh1236I	CGCG	3 cut(s)	77, 531, 1443
Bsh1285I	CGRYCG	1 cut(s)	1513
BshFI	GGCC	9 cut(s)	170, 183, 192, 204, 261, 296, 769, 879, 907
BsiEI	CGRYCG	1 cut(s)	1513
BsiHKAI	GWGCWC	3 cut(s)	287, 573, 1056
BsiSI	CCGG	3 cut(s)	179, 653, 1345
BslFI	GGGAC	3 cut(s)	267, 736, 1249
BslI	CCNNNNNNNGG	6 cut(s)	166, 200, 211, 214, 549, 852
BsmFI	GGGAC	3 cut(s)	267, 736, 1249
BsnI	GGCC	9 cut(s)	170, 183, 192, 204, 261, 296, 769, 879, 907
Bsp120I	GGGCCC	1 cut(s)	181
Bsp1286I	GDGCHC	4 cut(s)	185, 287, 573, 1056
Bsp143I	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
Bsp1720I	GCTNAGC	2 cut(s)	1055, 1227
Bsp19I	CCATGG	4 cut(s)	210, 585, 902, 1001
BspANI	GGCC	9 cut(s)	170, 183, 192, 204, 261, 296, 769, 879, 907
BspCNI	CTCAG	3 cut(s)	607, 828, 1068
BspFNI	CGCG	3 cut(s)	77, 531, 1443
BspLI	GGNNCC	4 cut(s)	183, 193, 260, 1300
BspPI	GGATC	2 cut(s)	509, 863
BspQI	GCTCTTC	1 cut(s)	1200
BsrDI	GCAATG	4 cut(s)	735, 1086, 1482, 1545
BsrI	ACTGG	1 cut(s)	262
BssECI	CCNNGG	9 cut(s)	30, 75, 160, 199, 210, 297, 585, 902, 1001
BssHII	GCGCGC	1 cut(s)	529
BssMI	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
BssNI	GRCGYC	1 cut(s)	219
BssSI	CACGAG	1 cut(s)	873
BssT1I	CCWWGG	5 cut(s)	199, 210, 585, 902, 1001
Bst2BI	CACGAG	1 cut(s)	873
Bst2UI	CCWGG	1 cut(s)	162
Bst4CI	ACNGT	2 cut(s)	11, 1511
Bst6I	CTCTTC	2 cut(s)	749, 1200
BstACI	GRCGYC	1 cut(s)	219
BstAPI	GCANNNNNTGC	2 cut(s)	12, 1283
BstBAI	YACGTR	2 cut(s)	231, 576
BstC8I	GCNNGC	6 cut(s)	287, 316, 531, 1061, 1434, 1454
BstDEI	CTNAG	4 cut(s)	615, 815, 1055, 1227
BstDSI	CCRYGG	6 cut(s)	75, 210, 297, 585, 902, 1001
BstENI	CCTNNNNNAGG	1 cut(s)	547
BstF5I	GGATG	5 cut(s)	40, 370, 997, 1021, 1353
BstFNI	CGCG	3 cut(s)	77, 531, 1443
BstHHI	GCGC	4 cut(s)	479, 531, 533, 1105
BstKTI	GATC	7 cut(s)	387, 504, 567, 607, 858, 934, 1291
BstMBI	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
BstMCI	CGRYCG	1 cut(s)	1513
BstMWI	GCNNNNNNNGC	8 cut(s)	12, 21, 104, 189, 539, 1060, 1283, 1449
BstNI	CCWGG	1 cut(s)	162
BstNSI	RCATGY	1 cut(s)	1456
BstSCI	CCNGG	2 cut(s)	160, 178
BstSLI	GKGCMC	3 cut(s)	185, 287, 573
BstUI	CGCG	3 cut(s)	77, 531, 1443
BstV1I	GCAGC	5 cut(s)	82, 418, 520, 526, 1289
BstX2I	RGATCY	3 cut(s)	604, 855, 931
BstYI	RGATCY	3 cut(s)	604, 855, 931
BsuRI	GGCC	9 cut(s)	170, 183, 192, 204, 261, 296, 769, 879, 907
BtgI	CCRYGG	6 cut(s)	75, 210, 297, 585, 902, 1001
BtsCI	GGATG	5 cut(s)	40, 370, 997, 1021, 1353
BtsI	GCAGTG	1 cut(s)	1371
BtsIMutI	CAGTG	2 cut(s)	823, 1371
Cac8I	GCNNGC	6 cut(s)	287, 316, 531, 1061, 1434, 1454
CfoI	GCGC	4 cut(s)	479, 531, 533, 1105
Cfr13I	GGNCC	5 cut(s)	181, 182, 191, 259, 767
Cfr42I	CCGCGG	1 cut(s)	78
CseI	GACGC	1 cut(s)	1505
Csp6I	GTAC	2 cut(s)	232, 380
CspCI	CAANNNNNGTGG	2 cut(s)	399, 434
CviAII	CATG	9 cut(s)	211, 388, 466, 586, 799, 903, 1002, 1453, 1457
CviQI	GTAC	2 cut(s)	232, 380
DdeI	CTNAG	4 cut(s)	615, 815, 1055, 1227
DpnI	GATC	7 cut(s)	386, 503, 566, 606, 857, 933, 1290
DpnII	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
DraI	TTTAAA	1 cut(s)	1075
DrdI	GACNNNNNNGTC	1 cut(s)	1243
DriI	GACNNNNNGTC	1 cut(s)	448
DseDI	GACNNNNNNGTC	1 cut(s)	1243
Eam1104I	CTCTTC	2 cut(s)	749, 1200
Eam1105I	GACNNNNNGTC	1 cut(s)	448
EarI	CTCTTC	2 cut(s)	749, 1200
EciI	GGCGGA	1 cut(s)	133
Eco130I	CCWWGG	5 cut(s)	199, 210, 585, 902, 1001
Eco24I	GRGCYC	1 cut(s)	185
Eco32I	GATATC	1 cut(s)	1471
Eco57I	CTGAAG	1 cut(s)	1232
Eco72I	CACGTG	1 cut(s)	576
EcoNI	CCTNNNNNAGG	1 cut(s)	547
EcoO109I	RGGNCCY	1 cut(s)	182
EcoRI	GAATTC	1 cut(s)	952
EcoRII	CCWGG	1 cut(s)	160
EcoRV	GATATC	1 cut(s)	1471
EcoT14I	CCWWGG	5 cut(s)	199, 210, 585, 902, 1001
EcoT22I	ATGCAT	2 cut(s)	393, 1458
EcoT38I	GRGCYC	1 cut(s)	185
ErhI	CCWWGG	5 cut(s)	199, 210, 585, 902, 1001
FaeI	CATG	9 cut(s)	214, 391, 469, 589, 802, 906, 1005, 1456, 1460
FalI	AAGNNNNNCTT	2 cut(s)	1089, 1121
FaqI	GGGAC	3 cut(s)	267, 736, 1249
FatI	CATG	9 cut(s)	210, 387, 465, 585, 798, 902, 1001, 1452, 1456
FauI	CCCGC	2 cut(s)	1111, 1397
FokI	GGATG	5 cut(s)	47, 377, 984, 1028, 1360
FriOI	GRGCYC	1 cut(s)	185
FspBI	CTAG	2 cut(s)	200, 1427
FspI	TGCGCA	1 cut(s)	478
GlaI	GCGC	4 cut(s)	478, 530, 532, 1104
GsaI	CCCAGC	2 cut(s)	120, 254
HaeIII	GGCC	9 cut(s)	170, 183, 192, 204, 261, 296, 769, 879, 907
HapII	CCGG	3 cut(s)	179, 653, 1345
HgaI	GACGC	1 cut(s)	1505
HhaI	GCGC	4 cut(s)	479, 531, 533, 1105
Hin1I	GRCGYC	1 cut(s)	219
Hin1II	CATG	9 cut(s)	214, 391, 469, 589, 802, 906, 1005, 1456, 1460
Hin6I	GCGC	4 cut(s)	477, 529, 531, 1103
HinP1I	GCGC	4 cut(s)	477, 529, 531, 1103
HinfI	GANTC	1 cut(s)	842
HpaII	CCGG	3 cut(s)	179, 653, 1345
HphI	GGTGA	4 cut(s)	71, 122, 149, 355
Hpy166II	GTNNAC	4 cut(s)	223, 285, 346, 571
Hpy188I	TCNGA	6 cut(s)	564, 847, 958, 1132, 1249, 1475
Hpy188III	TCNNGA	5 cut(s)	873, 1040, 1093, 1414, 1559
Hpy8I	GTNNAC	4 cut(s)	223, 285, 346, 571
Hpy99I	CGWCG	2 cut(s)	50, 1420
HpyAV	CCTTC	6 cut(s)	150, 841, 918, 991, 1387, 1458
HpyCH4III	ACNGT	2 cut(s)	11, 1511
HpyCH4IV	ACGT	3 cut(s)	219, 230, 575
HpyCH4V	TGCA	8 cut(s)	24, 285, 318, 391, 571, 740, 1336, 1456
HpyF10VI	GCNNNNNNNGC	8 cut(s)	12, 21, 104, 189, 539, 1060, 1283, 1449
HpyF3I	CTNAG	4 cut(s)	615, 815, 1055, 1227
HpySE526I	ACGT	3 cut(s)	219, 230, 575
Hsp92I	GRCGYC	1 cut(s)	219
Hsp92II	CATG	9 cut(s)	214, 391, 469, 589, 802, 906, 1005, 1456, 1460
HspAI	GCGC	4 cut(s)	477, 529, 531, 1103
KspI	CCGCGG	1 cut(s)	78
Kzo9I	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
LguI	GCTCTTC	1 cut(s)	1200
LmnI	GCTCC	3 cut(s)	247, 431, 526
Lsp1109I	GCAGC	5 cut(s)	82, 418, 520, 526, 1289
LweI	GCATC	4 cut(s)	25, 400, 1273, 1381
MaeI	CTAG	2 cut(s)	200, 1427
MaeII	ACGT	3 cut(s)	219, 230, 575
MaeIII	GTNAC	4 cut(s)	59, 271, 661, 1583
MalI	GATC	7 cut(s)	386, 503, 566, 606, 857, 933, 1290
MboI	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
MboII	GAAGA	8 cut(s)	365, 766, 850, 955, 1148, 1217, 1370, 1585
MflI	RGATCY	3 cut(s)	604, 855, 931
MhlI	GDGCHC	4 cut(s)	185, 287, 573, 1056
MmeI	TCCRAC	1 cut(s)	1405
Mph1103I	ATGCAT	2 cut(s)	393, 1458
MroXI	GAANNNNTTC	1 cut(s)	1088
MseI	TTAA	7 cut(s)	993, 1008, 1065, 1074, 1268, 1518, 1600
MslI	CAYNNNNRTG	1 cut(s)	1389
MspA1I	CMGCKG	4 cut(s)	77, 539, 1280, 1404
MspI	CCGG	3 cut(s)	179, 653, 1345
MspR9I	CCNGG	2 cut(s)	162, 180
MvaI	CCWGG	1 cut(s)	162
MvnI	CGCG	3 cut(s)	77, 531, 1443
MwoI	GCNNNNNNNGC	8 cut(s)	12, 21, 104, 189, 539, 1060, 1283, 1449
NciI	CCSGG	1 cut(s)	180
NcoI	CCATGG	4 cut(s)	210, 585, 902, 1001
NdeII	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
NlaIII	CATG	9 cut(s)	214, 391, 469, 589, 802, 906, 1005, 1456, 1460
NlaIV	GGNNCC	4 cut(s)	183, 193, 260, 1300
NmuCI	GTSAC	2 cut(s)	59, 1583
NsbI	TGCGCA	1 cut(s)	478
NsiI	ATGCAT	2 cut(s)	393, 1458
NspI	RCATGY	1 cut(s)	1456
PaeI	GCATGC	1 cut(s)	1456
PauI	GCGCGC	1 cut(s)	529
PciSI	GCTCTTC	1 cut(s)	1200
PdmI	GAANNNNTTC	1 cut(s)	1088
PfeI	GAWTC	1 cut(s)	842
PmaCI	CACGTG	1 cut(s)	576
PmlI	CACGTG	1 cut(s)	576
Ppu21I	YACGTR	2 cut(s)	231, 576
PshBI	ATTAAT	2 cut(s)	1268, 1518
Psp6I	CCWGG	1 cut(s)	160
PspCI	CACGTG	1 cut(s)	576
PspFI	CCCAGC	2 cut(s)	116, 250
PspGI	CCWGG	1 cut(s)	160
PspN4I	GGNNCC	4 cut(s)	183, 193, 260, 1300
PspOMI	GGGCCC	1 cut(s)	181
PspPI	GGNCC	5 cut(s)	181, 182, 191, 259, 767
PsuI	RGATCY	3 cut(s)	604, 855, 931
PteI	GCGCGC	1 cut(s)	529
PvuII	CAGCTG	1 cut(s)	1280
RsaI	GTAC	2 cut(s)	233, 381
RsaNI	GTAC	2 cut(s)	232, 380
RseI	CAYNNNNRTG	1 cut(s)	1389
SacII	CCGCGG	1 cut(s)	78
SapI	GCTCTTC	1 cut(s)	1200
SaqAI	TTAA	7 cut(s)	993, 1008, 1065, 1074, 1268, 1518, 1600
Sau3AI	GATC	7 cut(s)	384, 501, 564, 604, 855, 931, 1288
Sau96I	GGNCC	5 cut(s)	181, 182, 191, 259, 767
ScrFI	CCNGG	2 cut(s)	162, 180
SduI	GDGCHC	4 cut(s)	185, 287, 573, 1056
SfaNI	GCATC	4 cut(s)	25, 400, 1273, 1381
SfiI	GGCCNNNNNGGCC	1 cut(s)	189
Sfr303I	CCGCGG	1 cut(s)	78
SgrBI	CCGCGG	1 cut(s)	78
SmiMI	CAYNNNNRTG	1 cut(s)	1389
SmlI	CTYRAG	3 cut(s)	425, 608, 921
SmoI	CTYRAG	3 cut(s)	425, 608, 921
SphI	GCATGC	1 cut(s)	1456
SspMI	CTAG	2 cut(s)	200, 1427
StyD4I	CCNGG	2 cut(s)	160, 178
StyI	CCWWGG	5 cut(s)	199, 210, 585, 902, 1001
TaaI	ACNGT	2 cut(s)	11, 1511
TaiI	ACGT	3 cut(s)	222, 233, 578
TaqI	TCGA	4 cut(s)	495, 777, 1389, 1513
TauI	GCSGC	5 cut(s)	77, 173, 207, 539, 1582
TfiI	GAWTC	1 cut(s)	842
Tru1I	TTAA	7 cut(s)	993, 1008, 1065, 1074, 1268, 1518, 1600
Tru9I	TTAA	7 cut(s)	993, 1008, 1065, 1074, 1268, 1518, 1600
TscAI	CASTG	2 cut(s)	823, 1378
TseFI	GTSAC	2 cut(s)	59, 1583
TseI	GCWGC	5 cut(s)	95, 406, 533, 539, 1277
Tsp45I	GTSAC	2 cut(s)	59, 1583
TspDTI	ATGAA	5 cut(s)	446, 657, 1097, 1309, 1542
TspGWI	ACGGA	3 cut(s)	539, 670, 1311
TspRI	CASTG	2 cut(s)	823, 1378
VneI	GTGCAC	2 cut(s)	283, 569
VspI	ATTAAT	2 cut(s)	1268, 1518
XagI	CCTNNNNNAGG	1 cut(s)	547
XapI	RAATTY	3 cut(s)	619, 952, 1529
XceI	RCATGY	1 cut(s)	1456
XcmI	CCANNNNNNNNNTGG	1 cut(s)	337
XmaJI	CCTAGG	1 cut(s)	199
XmnI	GAANNNNTTC	1 cut(s)	1088
XspI	CTAG	2 cut(s)	200, 1427
ZraI	GACGTC	1 cut(s)	220
Zsp2I	ATGCAT	2 cut(s)	393, 1458

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.