MD03G1174700.v1.1

cyclin-dependent protein kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012953
Physical Location & Seq
Reverse (-)
23893554 .. 23893964
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1174700.v1.1.491

Sequence Viewer

Length: 411 bp
ATGACTCCGTCTGCAAGAACAAGATCAAAGACAAAAACGACAAGAGAGGCAAAAACAAGAACTCATTTCAAGAAGAAGCTTGGCCGGCCAAAGACTGCTACTGGTCAAGATATTGCAACTCCGACGACTTCGCCTTGGAATTCATCCTCCGTGGTCTGCAACGATGCTCCGAAGATTCAAGGTGGCGTAGATGATTTGGAGGCTGTTGACGATGTCTCTACTACAAGTGCCTGCTCCACACCGAAAGCTCAGAGATTTCGGATACCGGAGATTTTGACCTGTCCACCGGCGCCTAAGAAGAAGAGGGTGATTTCATCAAACTTCTTGTTTCGGAGATCTCCAATCGCCTTCTTTGCTCCGCCGGATTTAGAGCTCTTCTTCTGCTTTGCAAATGGCGGTATTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.84

Weight (kDa)

10.03

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51355 AT3G20898
fragaria_vesca FvH4_3g28800
malus_domestica MD03G1174700.v1.1 MD11G1192700.v1.1
prunus_persica Prupe.4G249800_v2.0.a1
pyrus_communis pycom03g13210 pycom11g16830
rosa_chinensis RchiOBHm_Chr5g0052211
rosa_laevigata RLG00000034836
rosa_multiflora Rmu_sc0006157.1_g000012
rosa_roxburghii Rroxscaffold_1G00028030
rosa_rugosa Rorug05G0274100
rosa_samantha Rh5AG346600 Rh5BG357200 Rh5CG382800 Rh5DG372700
rosa_wichuraiana Rw5G032730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 289
AciI CCGC 2 cut(s) 359, 396
AcoI YGGCCR 2 cut(s) 82, 86
AcsI RAATTY 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 290
AgsI TTSAA 2 cut(s) 70, 179
AluBI AGCT 3 cut(s) 79, 248, 373
AluI AGCT 3 cut(s) 79, 248, 373
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 220
AoxI GGCC 2 cut(s) 82, 86
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 319
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 375
BciVI GTATCC 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 220
BfoI RGCGCY 1 cut(s) 293
BfuI GTATCC 1 cut(s) 255
BglII AGATCT 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 291
BmsI GCATC 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 134, 150
BsaWI WCCGGW 1 cut(s) 265
Bse118I RCCGGY 2 cut(s) 84, 286
Bse1I ACTGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 134, 150
BseGI GGATG 1 cut(s) 143
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 106
BshFI GGCC 2 cut(s) 84, 88
BshNI GGYRCC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 375
BsiSI CCGG 4 cut(s) 85, 266, 287, 362
BsmAI GTCTC 1 cut(s) 220
BsnI GGCC 2 cut(s) 84, 88
Bsp1286I GDGCHC 1 cut(s) 375
Bsp143I GATC 2 cut(s) 23, 335
BspACI CCGC 2 cut(s) 359, 396
BspANI GGCC 2 cut(s) 84, 88
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 289
BsrFI RCCGGY 2 cut(s) 84, 286
BsrI ACTGG 1 cut(s) 106
BssAI RCCGGY 2 cut(s) 84, 286
BssECI CCNNGG 2 cut(s) 134, 150
BssMI GATC 2 cut(s) 23, 335
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 134
Bst6I CTCTTC 2 cut(s) 296, 380
BstACI GRCGYC 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 86, 232
BstDEI CTNAG 2 cut(s) 249, 294
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 2 cut(s) 26, 338
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 23, 335
BstMWI GCNNNNNNNGC 2 cut(s) 85, 353
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuI GTATCC 1 cut(s) 255
BsuRI GGCC 2 cut(s) 84, 88
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 86, 232
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 2 cut(s) 84, 286
CviJI RGCY 6 cut(s) 79, 84, 88, 203, 248, 373
CviKI_1 RGCY 6 cut(s) 79, 84, 88, 203, 248, 373
DdeI CTNAG 2 cut(s) 249, 294
DinI GGCGCC 1 cut(s) 291
DpnI GATC 2 cut(s) 25, 337
DpnII GATC 2 cut(s) 23, 335
EaeI YGGCCR 2 cut(s) 82, 86
Eam1104I CTCTTC 2 cut(s) 296, 380
EarI CTCTTC 2 cut(s) 296, 380
EciI GGCGGA 1 cut(s) 348
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 375
Eco53kI GAGCTC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 375
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 134
FokI GGATG 1 cut(s) 130
FriOI GRGCYC 1 cut(s) 375
FseI GGCCGGCC 1 cut(s) 88
GlaI GCGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 2 cut(s) 84, 88
HapII CCGG 4 cut(s) 85, 266, 287, 362
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 290
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 2 cut(s) 4, 175
HpaII CCGG 4 cut(s) 85, 266, 287, 362
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 208, 284
Hpy188I TCNGA 5 cut(s) 123, 171, 252, 261, 333
Hpy188III TCNNGA 2 cut(s) 70, 107
Hpy8I GTNNAC 2 cut(s) 208, 284
Hpy99I CGWCG 1 cut(s) 127
HpyAV CCTTC 1 cut(s) 358
HpyCH4V TGCA 4 cut(s) 14, 116, 159, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 353
HpyF3I CTNAG 2 cut(s) 249, 294
Hsp92I GRCGYC 1 cut(s) 290
HspAI GCGC 1 cut(s) 290
KasI GGCGCC 1 cut(s) 289
KroI GCCGGC 1 cut(s) 84
KroNI GCCGGC 1 cut(s) 86
Kzo9I GATC 2 cut(s) 23, 335
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 3 cut(s) 172, 239, 361
LpnPI CCDG 7 cut(s) 87, 98, 244, 279, 292, 300, 375
LweI GCATC 1 cut(s) 154
MalI GATC 2 cut(s) 25, 337
MboI GATC 2 cut(s) 23, 335
MboII GAAGA 6 cut(s) 85, 184, 310, 313, 367, 370
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 1 cut(s) 139
Mly113I GGCGCC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 4 cut(s) 40, 157, 193, 297
MroNI GCCGGC 1 cut(s) 84
MseI TTAA 1 cut(s) 409
MspI CCGG 4 cut(s) 85, 266, 287, 362
MwoI GCNNNNNNNGC 2 cut(s) 85, 353
NaeI GCCGGC 1 cut(s) 86
NarI GGCGCC 1 cut(s) 290
NdeII GATC 2 cut(s) 23, 335
NgoMIV GCCGGC 1 cut(s) 84
NlaIV GGNNCC 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 380
PdiI GCCGGC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 175
PflFI GACNNNGTC 2 cut(s) 7, 212
PluTI GGCGCC 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 291
PsuI RGATCY 1 cut(s) 335
PsyI GACNNNGTC 2 cut(s) 7, 212
RigI GGCCGGCC 1 cut(s) 88
SacI GAGCTC 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 1 cut(s) 409
Sau3AI GATC 2 cut(s) 23, 335
SduI GDGCHC 1 cut(s) 375
SetI ASST 5 cut(s) 81, 184, 250, 281, 375
SfaNI GCATC 1 cut(s) 154
SfoI GGCGCC 1 cut(s) 291
SgrAI CRCCGGYG 1 cut(s) 286
Sse9I AATT 1 cut(s) 139
SsiI CCGC 2 cut(s) 359, 396
SspDI GGCGCC 1 cut(s) 289
SstI GAGCTC 1 cut(s) 375
StyI CCWWGG 1 cut(s) 134
TasI AATT 1 cut(s) 139
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 132, 303
TspGWI ACGGA 1 cut(s) 139
Tth111I GACNNNGTC 2 cut(s) 7, 212
XapI RAATTY 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.