MD03G1183000.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018892
Physical Location & Seq
Reverse (-)
24957742 .. 24958710
969 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1183000.v1.1.491

Sequence Viewer

Length: 564 bp
ATGAATACCCTTTTCCTCACAGTTTTCCTTTTCCTCATCCCCATCTTGCTTCTCTTTGCAAGGAGAAAAAGATCACCAAAAAGGGTTCCTCCAGGCTCATTAGGATTACCCATAATCGGCCAAAGCCTCGGTCTTCTCCGAGCCATGCGTGCCAACACCGCCGAAAAATGGCTTCAACAAAGAATCGAAAAGTACGGCCCAGTTTCAAAGCTTAGTCTCTTTGGCAAACCAACGGTCTTCATTCATGGACCTGCTGCAAACAAGTTCATATTCGCCAGTGATGACAGCACCATTTCCAGTCAACAAGTTGAGTCTATCCGTCTACTTTTGGGTGACCGGAATATACTGGAGCTGAGCGGCGACGATCACAAGCGCGTCAGAAGTGCTCTTATGTTGTTCTTGAAGCCCGAGTCACTCAAGCAGTATGTGGGAAAGATGGACCAAGAAATCAGGAGACACCTTGAGATGCATTGGCATGGCAAGCAACAAATAACTGTACGCCCAAGTGGTCTTGCCCCTGATGAAAAACCTCACATTCAACATAATATGCTCCCTACTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

188

Amino Acids

21.31

Weight (kDa)

10.39

Isoelectric Point (pI)

69.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 156 3e-07 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018892)

Species Orthologous Gene IDs
malus_domestica MD03G1183000.v1.1 MD03G1204900.v1.1
rosa_multiflora Rmu_ssc0000410.1_g000039
rosa_roxburghii Rroxscaffold_1G00040340 Rroxscaffold_1G00041970
rosa_rugosa Rorug05G0176700
rosa_samantha Rh5BG268000 Rh5DG274800 Rh5DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 259
AccBSI CCGCTC 1 cut(s) 357
AccI GTMKAC 1 cut(s) 322
AccII CGCG 1 cut(s) 375
AciI CCGC 2 cut(s) 159, 357
AcoI YGGCCR 1 cut(s) 118
AfaI GTAC 2 cut(s) 194, 498
AfiI CCNNNNNNNGG 2 cut(s) 116, 168
AgsI TTSAA 4 cut(s) 176, 207, 403, 539
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 2 cut(s) 211, 352
AluI AGCT 2 cut(s) 211, 352
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 221, 448
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 2 cut(s) 118, 196
ApeKI GCWGC 1 cut(s) 254
ArsI GACNNNNNNTTYG 4 cut(s) 115, 147, 219, 251
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 3 cut(s) 197, 248, 439
AsuHPI GGTGA 2 cut(s) 66, 344
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 248, 439
BbsI GAAGAC 2 cut(s) 125, 229
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 50, 430
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 93
BcoDI GTCTC 2 cut(s) 221, 448
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 2 cut(s) 255, 358
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 2 cut(s) 256, 359
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 2 cut(s) 248, 439
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 197, 248, 439
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 93
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 2 cut(s) 125, 229
BpmI CTGGAG 2 cut(s) 75, 368
Bpu1102I GCTNAGC 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 401, 482
BsaJI CCNNGG 1 cut(s) 127
BsaWI WCCGGW 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 168
Bse1I ACTGG 4 cut(s) 200, 276, 297, 351
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 116, 168
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 4 cut(s) 200, 276, 297, 351
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 2 cut(s) 120, 198
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 1 cut(s) 337
BslI CCNNNNNNNGG 2 cut(s) 116, 168
BsmAI GTCTC 2 cut(s) 221, 448
BsnI GGCC 2 cut(s) 120, 198
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 2 cut(s) 71, 364
Bsp1720I GCTNAGC 1 cut(s) 353
BspACI CCGC 2 cut(s) 159, 357
BspANI GGCC 2 cut(s) 120, 198
BspCNI CTCAG 1 cut(s) 345
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 87
BspMI ACCTGC 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 357
BsrI ACTGG 4 cut(s) 200, 276, 297, 351
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 2 cut(s) 71, 364
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 3 cut(s) 22, 235, 496
BstC8I GCNNGC 2 cut(s) 150, 482
BstDEI CTNAG 2 cut(s) 212, 353
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 1 cut(s) 36
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 2 cut(s) 74, 367
BstMAI GTCTC 2 cut(s) 221, 448
BstMBI GATC 2 cut(s) 71, 364
BstMWI GCNNNNNNNGC 3 cut(s) 149, 158, 481
BstNI CCWGG 1 cut(s) 93
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 91
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 2 cut(s) 125, 229
BsuRI GGCC 2 cut(s) 120, 198
BtsCI GGATG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 283
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 2 cut(s) 150, 482
CfoI GCGC 1 cut(s) 375
Cfr13I GGNCC 3 cut(s) 197, 248, 439
CseI GACGC 1 cut(s) 364
Csp6I GTAC 2 cut(s) 193, 497
CviAII CATG 3 cut(s) 145, 245, 476
CviJI RGCY 9 cut(s) 96, 120, 126, 143, 172, 198, 211, 352, 406
CviKI_1 RGCY 9 cut(s) 96, 120, 126, 143, 172, 198, 211, 352, 406
CviQI GTAC 2 cut(s) 193, 497
DdeI CTNAG 2 cut(s) 212, 353
DpnI GATC 2 cut(s) 73, 366
DpnII GATC 2 cut(s) 71, 364
EaeI YGGCCR 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 248, 439
Eco88I CYCGRG 1 cut(s) 407
Eco91I GGTNACC 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 471
FaeI CATG 3 cut(s) 148, 248, 479
FatI CATG 3 cut(s) 144, 244, 475
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 255, 358
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 255, 358
GlaI GCGC 1 cut(s) 374
GluI GCNGC 2 cut(s) 255, 358
GsuI CTGGAG 2 cut(s) 75, 368
HaeIII GGCC 2 cut(s) 120, 198
HapII CCGG 1 cut(s) 337
HgaI GACGC 1 cut(s) 364
HhaI GCGC 1 cut(s) 375
Hin1II CATG 3 cut(s) 148, 248, 479
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 3 cut(s) 183, 311, 410
HpaII CCGG 1 cut(s) 337
HphI GGTGA 2 cut(s) 66, 344
Hpy166II GTNNAC 2 cut(s) 302, 323
Hpy188I TCNGA 2 cut(s) 140, 380
Hpy188III TCNNGA 2 cut(s) 400, 451
Hpy8I GTNNAC 2 cut(s) 302, 323
Hpy99I CGWCG 1 cut(s) 365
HpyCH4III ACNGT 3 cut(s) 22, 235, 496
HpyCH4V TGCA 3 cut(s) 59, 257, 469
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 158, 481
HpyF3I CTNAG 2 cut(s) 212, 353
Hsp92II CATG 3 cut(s) 148, 248, 479
HspAI GCGC 1 cut(s) 373
Kzo9I GATC 2 cut(s) 71, 364
LmnI GCTCC 2 cut(s) 349, 555
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 332, 411
MalI GATC 2 cut(s) 73, 366
MbiI CCGCTC 1 cut(s) 357
MboI GATC 2 cut(s) 71, 364
MboII GAAGA 2 cut(s) 125, 229
MhlI GDGCHC 1 cut(s) 388
MlyI GAGTC 2 cut(s) 320, 419
MnlI CCTC 5 cut(s) 26, 44, 99, 137, 540
Mph1103I ATGCAT 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 3 cut(s) 149, 158, 481
NdeII GATC 2 cut(s) 71, 364
NlaIII CATG 3 cut(s) 148, 248, 479
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 332, 411
NsiI ATGCAT 1 cut(s) 471
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 2 cut(s) 256, 359
PleI GAGTC 2 cut(s) 319, 418
PpsI GAGTC 2 cut(s) 319, 418
Psp6I CCWGG 1 cut(s) 91
PspEI GGTNACC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 3 cut(s) 197, 248, 439
RsaI GTAC 2 cut(s) 194, 498
RsaNI GTAC 2 cut(s) 193, 497
RseI CAYNNNNRTG 1 cut(s) 474
SatI GCNGC 2 cut(s) 255, 358
Sau3AI GATC 2 cut(s) 71, 364
Sau96I GGNCC 3 cut(s) 197, 248, 439
SchI GAGTC 2 cut(s) 320, 419
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 1 cut(s) 388
SetI ASST 5 cut(s) 213, 253, 354, 462, 532
SfaNI GCATC 1 cut(s) 456
SinI GGWCC 2 cut(s) 248, 439
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 2 cut(s) 416, 461
SmoI CTYRAG 2 cut(s) 416, 461
SsiI CCGC 2 cut(s) 159, 357
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 22, 235, 496
TaqI TCGA 1 cut(s) 186
TaqII GACCGA 1 cut(s) 119
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 183
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 332, 411
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 2 cut(s) 332, 411
TspDTI ATGAA 5 cut(s) 17, 229, 233, 256, 537
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 283
VpaK11BI GGWCC 2 cut(s) 248, 439
XmiI GTMKAC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.