Rh5DG275800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018892
Physical Location & Seq
Reverse (-)
33785516 .. 33786313
798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG275800.1

Sequence Viewer

Length: 582 bp
ATGGTGACTACTCTTTTCACAATTTTCCTCTTCCTCATTCCCATATACCTTCTCCTCAAGAGCATAAGAAAATCATCAGAAAGGCTTCCACCAGGTTCATTAGGACTGCCCCTAATAGGCCAGAGCCTTGATTTTCTCCGAGCGATGCGTGCCAACACTGCAGAGGATTGGCTTGAACAAAGGATCAGAAAGTATGGTCCAGTTTCAAAGCTAAGTCTCTTTGGCAAGCCAACAGTCTTCATCCATGGACAAGCTGCAAACAAGTTTATATTCACCAGTGATGGGAGCACCATTTCAGCCCAACAATCCGAGTCTATTCGTAGGATTTTGGGGGAACGGAGTATATCCGAGTTAAGTGCTGAGGATCACAAACGTGTCAGAAGTGCACTTATGCTATTCTTGAAGCCTGAGTCACTGAAGCAATATGTGTGGAAAATGGATGAAGAAATCAGGAAGCACCTTGAGATACACTGGCATGGCAAACAACAAGTAACAGTTATGCCCCTGATGAAGAACCTCACATTTAACATAATATCCTCTCTACTTTTTGGGCTTGAACGAGGAACTCGGCCGGAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

193

Amino Acids

22.2

Weight (kDa)

10.09

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 188 2.8e-09 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018892)

Species Orthologous Gene IDs
malus_domestica MD03G1183000.v1.1 MD03G1204900.v1.1
rosa_multiflora Rmu_ssc0000410.1_g000039
rosa_roxburghii Rroxscaffold_1G00040340 Rroxscaffold_1G00041970
rosa_rugosa Rorug05G0176700
rosa_samantha Rh5BG268000 Rh5DG274800 Rh5DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 191, 372
AcoI YGGCCR 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 437
AfiI CCNNNNNNNGG 2 cut(s) 116, 282
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 4 cut(s) 176, 207, 403, 557
AjnI CCWGG 1 cut(s) 91
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 2 cut(s) 211, 254
AluI AGCT 2 cut(s) 211, 254
Alw21I GWGCWC 2 cut(s) 290, 388
Alw26I GTCTC 1 cut(s) 221
Alw44I GTGCAC 1 cut(s) 384
AlwI GGATC 2 cut(s) 191, 372
AoxI GGCC 2 cut(s) 118, 569
ApaLI GTGCAC 1 cut(s) 384
ApeKI GCWGC 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 16, 265
AvaII GGWCC 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 2 cut(s) 290, 388
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 275
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 197
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 229
Bpu10I CCTNAGC 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 41, 482
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 282
Bse1I ACTGG 3 cut(s) 200, 276, 476
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 2 cut(s) 240, 445
BseLI CCNNNNNNNGG 2 cut(s) 116, 282
BseMII CTCAG 2 cut(s) 351, 399
BseNI ACTGG 3 cut(s) 200, 276, 476
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 388
BseX3I CGGCCG 1 cut(s) 569
BseXI GCAGC 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 572
BshFI GGCC 2 cut(s) 120, 571
BsiEI CGRYCG 1 cut(s) 572
BsiHKAI GWGCWC 2 cut(s) 290, 388
BsiSI CCGG 1 cut(s) 572
BslI CCNNNNNNNGG 2 cut(s) 116, 282
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 2 cut(s) 120, 571
Bsp1286I GDGCHC 2 cut(s) 290, 388
Bsp143I GATC 2 cut(s) 183, 364
Bsp19I CCATGG 1 cut(s) 244
BspANI GGCC 2 cut(s) 120, 571
BspCNI CTCAG 2 cut(s) 352, 400
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 2 cut(s) 191, 372
BsrI ACTGG 3 cut(s) 200, 276, 476
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 2 cut(s) 183, 364
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 235, 496
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 150, 227
BstDEI CTNAG 3 cut(s) 212, 360, 408
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 2 cut(s) 240, 445
BstKTI GATC 2 cut(s) 186, 367
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 183, 364
BstMCI CGRYCG 1 cut(s) 572
BstMWI GCNNNNNNNGC 2 cut(s) 149, 158
BstNI CCWGG 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 229
BstZI CGGCCG 1 cut(s) 569
BsuRI GGCC 2 cut(s) 120, 571
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 158
BtsCI GGATG 2 cut(s) 240, 445
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 4 cut(s) 156, 283, 413, 469
Cac8I GCNNGC 2 cut(s) 150, 227
Cfr13I GGNCC 1 cut(s) 197
CsiI ACCWGGT 1 cut(s) 91
CspCI CAANNNNNGTGG 2 cut(s) 410, 445
CviAII CATG 2 cut(s) 245, 476
DdeI CTNAG 3 cut(s) 212, 360, 408
DpnI GATC 2 cut(s) 185, 366
DpnII GATC 2 cut(s) 183, 364
EaeI YGGCCR 1 cut(s) 569
EagI CGGCCG 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EclXI CGGCCG 1 cut(s) 569
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 197
Eco52I CGGCCG 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 2 cut(s) 248, 479
FatI CATG 2 cut(s) 244, 475
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 2 cut(s) 227, 452
Fsp4HI GCNGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 2 cut(s) 120, 571
HapII CCGG 1 cut(s) 572
Hin1II CATG 2 cut(s) 248, 479
HinfI GANTC 2 cut(s) 311, 410
HpaII CCGG 1 cut(s) 572
HphI GGTGA 2 cut(s) 16, 265
Hpy166II GTNNAC 1 cut(s) 386
Hpy188I TCNGA 6 cut(s) 79, 140, 188, 310, 349, 380
Hpy188III TCNNGA 3 cut(s) 58, 400, 451
Hpy8I GTNNAC 1 cut(s) 386
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 235, 496
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 3 cut(s) 161, 257, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 158
HpyF3I CTNAG 3 cut(s) 212, 360, 408
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 2 cut(s) 248, 479
Kzo9I GATC 2 cut(s) 183, 364
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 9 cut(s) 78, 105, 134, 213, 289, 420, 436, 457, 518
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 135
MabI ACCWGGT 1 cut(s) 91
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 3 cut(s) 4, 411, 490
MalI GATC 2 cut(s) 185, 366
MboI GATC 2 cut(s) 183, 364
MboII GAAGA 4 cut(s) 22, 229, 455, 523
MhlI GDGCHC 2 cut(s) 290, 388
MluCI AATT 1 cut(s) 21
MlyI GAGTC 2 cut(s) 320, 419
MnlI CCTC 8 cut(s) 38, 44, 65, 157, 355, 527, 547, 554
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 2 cut(s) 353, 525
MslI CAYNNNNRTG 2 cut(s) 372, 474
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 149, 158
NcoI CCATGG 1 cut(s) 244
NdeII GATC 2 cut(s) 183, 364
NlaIII CATG 2 cut(s) 248, 479
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 4, 411
OliI CACNNNNGTG 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 2 cut(s) 319, 418
PpsI GAGTC 2 cut(s) 319, 418
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspPI GGNCC 1 cut(s) 197
PstI CTGCAG 1 cut(s) 163
RseI CAYNNNNRTG 2 cut(s) 372, 474
SaqAI TTAA 2 cut(s) 353, 525
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 2 cut(s) 183, 364
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 2 cut(s) 320, 419
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 2 cut(s) 290, 388
SetI ASST 7 cut(s) 51, 97, 213, 256, 376, 462, 519
SexAI ACCWGGT 1 cut(s) 91
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 372, 474
SmlI CTYRAG 2 cut(s) 56, 461
SmoI CTYRAG 2 cut(s) 56, 461
Sse9I AATT 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 235, 496
TaiI ACGT 1 cut(s) 376
TasI AATT 1 cut(s) 21
Tru1I TTAA 2 cut(s) 353, 525
Tru9I TTAA 2 cut(s) 353, 525
TscAI CASTG 4 cut(s) 163, 283, 420, 476
TseFI GTSAC 2 cut(s) 4, 411
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 2 cut(s) 4, 411
TspDTI ATGAA 4 cut(s) 87, 229, 456, 524
TspGWI ACGGA 1 cut(s) 352
TspRI CASTG 4 cut(s) 163, 283, 420, 476
VneI GTGCAC 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.