MD03G1204900.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018892
Physical Location & Seq
Forward (+)
28116773 .. 28117924
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1204900.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGATGCCCTTGTCCTCACAGTTTTCCTCTTCCTCATCCCCGTCTTCTTTCTCCTTACAAGAAGAAAAAAATCATCAGAACGGCTTCCTCCCGGCTCATTGGGACTACCCATTATAGGTCAAAGCCTTGGTTTTCTTGGAGCCCTGCGTGCCAACACTGCCGAAAAGTGGCTTGAACAAAGAATCGCAAAGTACGGTCCAGTTTCAAAGATGAGTCTCTTTGGCAAGCCAACAGTCTTCATTCATGGACAGGCTGCCAACAAGTTTGTATTCAACAATGATGGCACATCATTTACCAGTCAACAACCTGAGTCTACTCGTATGATTTTGGGCGACCGGAATATACTGGAACTGAGCGGCGAGGATCACAAGCGTGTCAGAAGTGCTCTTATGCTTTTCTTGAAGCCCGAGTCACTGAAGCGATATGTTGGAAAAATGGATGAAGAAATCAGGAATCATCTTGAGATGCACTGGCATGGCAAGCAAAAAATAACAGTTTTGCCCCTCATGAAGAACCTCACATTCAACATAATATGCTCTCTACTTTTTGGGGTTGAACGAGGACCTCGCAGAGACAAACTCATTGAGTGTTTCCGACATATGATAGAAGGAATCTGGTCGGTCCCTGTTAATTTGCCCTTCACGCGTTACAACCAAAGCCTCAAGGCGAGCAAGAAGGTTCAAAAAATGCTCAAGGAACTCGTATGTGAAAAGAGGGTACAACTTGAGCAAAAAACTACTTCTCCACTCCAAGACCTTATAACCTGCTTGCTTAACATTCGAAACATTGACAACGAAGAAGAATTAACGGAAAATGAGATCCTACACAATATCATGGTAGTTATGATCGCAGGATATGACACTTCATCTGTTGTGATTACTTTTCTTTTGCGGCTTCTAGCGAATGAACCTGCTATCCATGCAGCCGTTCTTCAAGGTATGCATAACGCATCCCGTGCCTACAACCTCACACATAACTATTTAACGCAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

332

Amino Acids

37.8

Weight (kDa)

9.51

Isoelectric Point (pI)

66.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 316 3.4e-31 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018892)

Species Orthologous Gene IDs
malus_domestica MD03G1183000.v1.1 MD03G1204900.v1.1
rosa_multiflora Rmu_ssc0000410.1_g000039
rosa_roxburghii Rroxscaffold_1G00040340 Rroxscaffold_1G00041970
rosa_rugosa Rorug05G0176700
rosa_samantha Rh5BG268000 Rh5DG274800 Rh5DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
Acc36I ACCTGC 2 cut(s) 773, 919
AccBSI CCGCTC 1 cut(s) 357
AccI GTMKAC 1 cut(s) 314
AccII CGCG 1 cut(s) 646
AciI CCGC 2 cut(s) 357, 892
AclWI GGATC 2 cut(s) 372, 814
AcuI CTGAAG 1 cut(s) 437
AfaI GTAC 2 cut(s) 194, 720
AfiI CCNNNNNNNGG 2 cut(s) 116, 168
AflIII ACRYGT 1 cut(s) 644
AgsI TTSAA 8 cut(s) 176, 207, 274, 403, 526, 557, 683, 935
AleI CACNNNNGTG 1 cut(s) 372
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 221, 567
AlwI GGATC 2 cut(s) 372, 814
Ama87I CYCGRG 1 cut(s) 407
ApeKI GCWGC 2 cut(s) 254, 923
Asp700I GAANNNNTTC 1 cut(s) 84
AspS9I GGNCC 3 cut(s) 197, 563, 622
AsuC2I CCSGG 1 cut(s) 93
AsuII TTCGAA 1 cut(s) 781
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 3 cut(s) 197, 563, 622
BanII GRGCYC 1 cut(s) 145
BbsI GAAGAC 2 cut(s) 37, 229
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 241, 935
BccI CCATC 1 cut(s) 275
BceAI ACGGC 2 cut(s) 98, 911
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 2 cut(s) 221, 567
BfaI CTAG 2 cut(s) 899, 994
BfuAI ACCTGC 2 cut(s) 773, 919
BisI GCNGC 4 cut(s) 255, 358, 893, 924
BlsI GCNGC 4 cut(s) 256, 359, 894, 925
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 3 cut(s) 197, 563, 622
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 197, 563, 622
BmiI GGNNCC 2 cut(s) 142, 624
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 456, 959
BpiI GAAGAC 2 cut(s) 37, 229
BplI GAGNNNNNCTC 2 cut(s) 564, 596
Bpu14I TTCGAA 1 cut(s) 781
BpuEI CTTGAG 4 cut(s) 482, 647, 677, 746
BpuMI CCSGG 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 127
BsaWI WCCGGW 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 168
Bse1I ACTGG 4 cut(s) 200, 297, 351, 476
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 4 cut(s) 10, 36, 445, 950
BseLI CCNNNNNNNGG 2 cut(s) 116, 168
BseMII CTCAG 2 cut(s) 300, 344
BseNI ACTGG 4 cut(s) 200, 297, 351, 476
BseXI GCAGC 2 cut(s) 241, 935
Bsh1236I CGCG 1 cut(s) 646
Bsh1285I CGRYCG 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 2 cut(s) 93, 337
BslFI GGGAC 2 cut(s) 117, 608
BslI CCNNNNNNNGG 2 cut(s) 116, 168
BsmAI GTCTC 2 cut(s) 221, 567
BsmFI GGGAC 2 cut(s) 117, 608
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 781
Bsp1286I GDGCHC 2 cut(s) 145, 388
Bsp143I GATC 3 cut(s) 364, 819, 846
BspACI CCGC 2 cut(s) 357, 892
BspCNI CTCAG 2 cut(s) 301, 345
BspFNI CGCG 1 cut(s) 646
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 2 cut(s) 142, 624
BspMI ACCTGC 2 cut(s) 773, 919
BspPI GGATC 2 cut(s) 372, 814
BspT104I TTCGAA 1 cut(s) 781
BsrBI CCGCTC 1 cut(s) 357
BsrI ACTGG 4 cut(s) 200, 297, 351, 476
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 3 cut(s) 364, 819, 846
BssT1I CCWWGG 1 cut(s) 127
Bst4CI ACNGT 4 cut(s) 22, 197, 235, 496
Bst6I CTCTTC 1 cut(s) 35
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBI TTCGAA 1 cut(s) 781
BstC8I GCNNGC 5 cut(s) 150, 227, 482, 670, 770
BstDEI CTNAG 2 cut(s) 309, 353
BstF5I GGATG 4 cut(s) 10, 36, 445, 950
BstFNI CGCG 1 cut(s) 646
BstKTI GATC 3 cut(s) 367, 822, 849
BstMAI GTCTC 2 cut(s) 221, 567
BstMBI GATC 3 cut(s) 364, 819, 846
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 6 cut(s) 149, 158, 481, 643, 920, 956
BstSCI CCNGG 1 cut(s) 91
BstUI CGCG 1 cut(s) 646
BstV1I GCAGC 2 cut(s) 241, 935
BstV2I GAAGAC 2 cut(s) 37, 229
BstX2I RGATCY 1 cut(s) 819
BstYI RGATCY 1 cut(s) 819
BtsCI GGATG 4 cut(s) 10, 36, 445, 950
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 3 cut(s) 156, 413, 469
BveI ACCTGC 2 cut(s) 773, 919
Cac8I GCNNGC 5 cut(s) 150, 227, 482, 670, 770
CciI TCATGA 1 cut(s) 507
Cfr13I GGNCC 3 cut(s) 197, 563, 622
Csp6I GTAC 2 cut(s) 193, 719
CviAII CATG 5 cut(s) 245, 476, 508, 835, 920
CviQI GTAC 2 cut(s) 193, 719
DdeI CTNAG 2 cut(s) 309, 353
DpnI GATC 3 cut(s) 366, 821, 848
DpnII GATC 3 cut(s) 364, 819, 846
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 3 cut(s) 197, 563, 622
Eco57I CTGAAG 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 945
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 5 cut(s) 248, 479, 511, 838, 923
FaqI GGGAC 2 cut(s) 117, 608
FatI CATG 5 cut(s) 244, 475, 507, 834, 919
FauNDI CATATG 1 cut(s) 600
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 4 cut(s) 255, 358, 893, 924
FokI GGATG 4 cut(s) 17, 23, 452, 937
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 4 cut(s) 255, 358, 893, 924
FspBI CTAG 2 cut(s) 899, 994
GluI GCNGC 4 cut(s) 255, 358, 893, 924
HapII CCGG 2 cut(s) 93, 337
Hin1II CATG 5 cut(s) 248, 479, 511, 838, 923
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HinfI GANTC 6 cut(s) 183, 214, 311, 410, 454, 612
HpaII CCGG 2 cut(s) 93, 337
Hpy166II GTNNAC 2 cut(s) 302, 315
Hpy188I TCNGA 3 cut(s) 79, 380, 596
Hpy188III TCNNGA 4 cut(s) 400, 451, 461, 508
Hpy8I GTNNAC 2 cut(s) 302, 315
HpyAV CCTTC 3 cut(s) 602, 649, 670
HpyCH4III ACNGT 4 cut(s) 22, 197, 235, 496
HpyCH4V TGCA 3 cut(s) 469, 923, 943
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 158, 481, 643, 920, 956
HpyF3I CTNAG 2 cut(s) 309, 353
Hsp92II CATG 5 cut(s) 248, 479, 511, 838, 923
Kzo9I GATC 3 cut(s) 364, 819, 846
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 2 cut(s) 241, 935
LweI GCATC 2 cut(s) 456, 959
MaeI CTAG 2 cut(s) 899, 994
MaeIII GTNAC 2 cut(s) 411, 647
MalI GATC 3 cut(s) 366, 821, 848
MbiI CCGCTC 1 cut(s) 357
MboI GATC 3 cut(s) 364, 819, 846
MboII GAAGA 9 cut(s) 22, 37, 75, 229, 455, 523, 809, 812, 923
MflI RGATCY 1 cut(s) 819
MhlI GDGCHC 2 cut(s) 145, 388
MluCI AATT 2 cut(s) 631, 803
MluI ACGCGT 1 cut(s) 644
MlyI GAGTC 3 cut(s) 223, 320, 419
MmeI TCCRAC 2 cut(s) 409, 619
Mph1103I ATGCAT 1 cut(s) 945
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 4 cut(s) 630, 774, 806, 983
MslI CAYNNNNRTG 2 cut(s) 372, 474
MspI CCGG 2 cut(s) 93, 337
MspR9I CCNGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 646
MwoI GCNNNNNNNGC 6 cut(s) 149, 158, 481, 643, 920, 956
NciI CCSGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 600
NdeII GATC 3 cut(s) 364, 819, 846
NlaIII CATG 5 cut(s) 248, 479, 511, 838, 923
NlaIV GGNNCC 2 cut(s) 142, 624
NmuCI GTSAC 1 cut(s) 411
NsiI ATGCAT 1 cut(s) 945
NspV TTCGAA 1 cut(s) 781
OliI CACNNNNGTG 1 cut(s) 372
PagI TCATGA 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 183, 454, 612
PkrI GCNGC 4 cut(s) 256, 359, 894, 925
PleI GAGTC 3 cut(s) 222, 319, 418
PpsI GAGTC 3 cut(s) 222, 319, 418
PpuMI RGGWCCY 1 cut(s) 563
PsiI TTATAA 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 142, 624
PspPI GGNCC 3 cut(s) 197, 563, 622
PspPPI RGGWCCY 1 cut(s) 563
PsuI RGATCY 1 cut(s) 819
RsaI GTAC 2 cut(s) 194, 720
RsaNI GTAC 2 cut(s) 193, 719
RseI CAYNNNNRTG 2 cut(s) 372, 474
SaqAI TTAA 4 cut(s) 630, 774, 806, 983
SatI GCNGC 4 cut(s) 255, 358, 893, 924
Sau3AI GATC 3 cut(s) 364, 819, 846
Sau96I GGNCC 3 cut(s) 197, 563, 622
SchI GAGTC 3 cut(s) 223, 320, 419
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 2 cut(s) 145, 388
SfaNI GCATC 2 cut(s) 456, 959
SfuI TTCGAA 1 cut(s) 781
SinI GGWCC 3 cut(s) 197, 563, 622
SmiMI CAYNNNNRTG 2 cut(s) 372, 474
SmlI CTYRAG 4 cut(s) 461, 662, 692, 725
SmoI CTYRAG 4 cut(s) 461, 662, 692, 725
Sse9I AATT 2 cut(s) 631, 803
SsiI CCGC 2 cut(s) 357, 892
SspMI CTAG 2 cut(s) 899, 994
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 4 cut(s) 22, 197, 235, 496
TaqI TCGA 1 cut(s) 781
TaqII GACCGA 1 cut(s) 610
TasI AATT 2 cut(s) 631, 803
TauI GCSGC 2 cut(s) 360, 895
TfiI GAWTC 3 cut(s) 183, 454, 612
Tru1I TTAA 4 cut(s) 630, 774, 806, 983
Tru9I TTAA 4 cut(s) 630, 774, 806, 983
TscAI CASTG 3 cut(s) 163, 420, 476
TseFI GTSAC 1 cut(s) 411
TseI GCWGC 2 cut(s) 254, 923
Tsp45I GTSAC 1 cut(s) 411
TspDTI ATGAA 6 cut(s) 229, 233, 456, 524, 855, 921
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 3 cut(s) 163, 420, 476
VpaK11BI GGWCC 3 cut(s) 197, 563, 622
XmiI GTMKAC 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 2 cut(s) 899, 994
Zsp2I ATGCAT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.