Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008406

Physical Location & Seq

Genomic Coordinates Reverse (-)

25682681 .. 25685985

Gene Structure 3305 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1187700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGTCCCTAACAATCTCACCCAACTCTCTCCAACCCTCTACATTTCAATTTACCTTTCATTCCTCCCAAAATTACAGCTTCACCCAGCCATGGCTTCCGCATCCGCTTATCCGTAAGCGACCTAGGACCCGAATTTCCTGCGTTTCGACCCGACCCAAAAGAAATCCGGGACCAAAGACCGAGGACCCGGAAGTCCGGGAGGTGGTTCGGACGCTCATGAGGAGCTTCAGTGACAAAGAGCCGTTGTTGAAGACGCTAAACAAGTACGTTAAGATTGTGAGGACGGAGCATTGCTTTATGCTCTTTGAAGAGCTTGGGAAGACTGATAAGTGGCTTCAGTGCTTGGAGGTGTTCAGATGGATGCAAAGACAGCGGTGGTACGTTGCTGATAACGGTGTGTATTCAAAATTGATCTCGGTTATGGGAAAGAAAGGCCAAACTCGGATGGCAATGTGGCTTTTCTCTGAGATGCGTAATAGTGGGTGCCGGCCTGATACTTCTGTCTACAATGCACTTATCTCAGCCCACTTAAACTCCAAGGACAAAGCAAAGGCTTTGGACAAAGCTCTTGGATACTTCAACAAGATGAAGGGAATGGAACGATGCCAACCCAGCATTGTGACGTACAACATTCTTTTGAGAGCTTTTGCACAATCTCGAAATGTAGAGAAAGTCAATGCTTTATTTAAGGATCTTGATGAGAGCGTTGCTTCCCCTGATATTTATACGTATAATGGTGTAATGGATGCATATGGGAAGAATGGGAATATAAGAGAAATGGAATCTGTGCTTTCTCGGATGAAAAGTAATCAGTGTAAGCCTGACATAATCACCTTTAATTTGCTGATTGATTCATATGGGAAGAAGCAACAATTCGATAAGATGGAACAGGTATTTAAGAGCTTACTTCGTTCCAAGGAGAAACCAACACTTCCAACGTTTAATTCAATGATCATAAATTATGGGAAGGCAAGGCTTATGGAGAAAGCGGAACATGTATTCCAGAAGATGACTGACATGAAATATACGCCAAGCTTCATAACATATGAGAGTCTTATCATGATGTATGGGTTCTGTGATTGTGTTTCAAAAGCTAGGGATGTATTTGAAAGGTTGGCTGATTCTGGAAGGGAGCTAAAGGTTTCAACGCTGAATGCGATGCTTGATGTTTACTGCATGAATGGTTTGCCCATGGAAGCTGATAAGCTTTTTGTCAGTGCAAATAGTATTGGGGTACGCCCAAATGTATCAACATATAAACTTCTATACAAGTATTACACTAAGGGCAACATGAAGGAGCTTCTCGAGAAACTGCTGAAGTCCATGGAAAATGATGGTATTGTCCCTAATAAGAGGTTCTTTCTAGAGGCTCTTGGGGCATTTTTCTCTTCACCAGGGAGTCCAGGCTCCGTGACTGCTACAACTGCTTTGAGCAGACAGCAGGATGGCGCAAAAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000373 GO:0000375 GO:0000377 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0031425 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

487

Amino Acids

55.83

Weight (kDa)

9.55

Isoelectric Point (pI)

37.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	133 - 176	7.1e-09	PPR repeat family
PPR_long	PF17177	141 - 274	7.2e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	164 - 217	2.4e-09	PPR repeat family
PPR_2	PF13041	204 - 252	2.3e-10	PPR repeat family
PPR_3	PF13812	228 - 288	2.9e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	239 - 285	2.3e-12	PPR repeat family
PPR	PF01535	242 - 272	8.3e-07	PPR repeat
PPR_2	PF13041	274 - 318	1.3e-07	PPR repeat family
PPR_2	PF13041	312 - 353	3.5e-06	PPR repeat family
PPR_2	PF13041	381 - 422	9.5e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008406)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G39620 AT4G39620
fragaria_vesca	FvH4_4g12030 FvH4_4g12030
malus_domestica	MD03G1187700.v1.1 MD11G1203200.v1.1
prunus_persica	Prupe.4G236100_v2.0.a1
pyrus_communis	pycom03g14050 pycom11g17630
rosa_chinensis	RchiOBHm_Chr4g0409671
rosa_laevigata	RLG00000008536
rosa_multiflora	Rmu_co8330861.1_g000001 Rmu_sc0008746.1_g000016 Rmu_sc0015305.1_g000009
rosa_roxburghii	Rroxscaffold_5G00354190
rosa_rugosa	Rorug04G0095900 Rorug04G0096000
rosa_samantha	Rh4AG157400 Rh4BG157000 Rh4CG167600 Rh4DG151400
rosa_wichuraiana	Rw4G012880 Rw4G012990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	191
AccB1I	GGYRCC	1 cut(s)	483
AccI	GTMKAC	1 cut(s)	504
AciI	CCGC	4 cut(s)	98, 104, 373, 989
AclI	AACGTT	1 cut(s)	938
AclWI	GGATC	1 cut(s)	699
AcsI	RAATTY	1 cut(s)	132
AcuI	CTGAAG	3 cut(s)	211, 320, 1337
AfaI	GTAC	4 cut(s)	266, 380, 626, 1236
AfiI	CCNNNNNNNGG	2 cut(s)	90, 202
AflIII	ACRYGT	1 cut(s)	994
AgsI	TTSAA	9 cut(s)	47, 250, 308, 405, 580, 948, 1089, 1109, 1146
AjnI	CCWGG	2 cut(s)	1393, 1402
AjuI	GAANNNNNNNTTGG	2 cut(s)	928, 960
AloI	GAACNNNNNNTCC	2 cut(s)	984, 1016
AlwI	GGATC	1 cut(s)	699
Ama87I	CYCGRG	1 cut(s)	1304
AoxI	GGCC	2 cut(s)	433, 488
ApoI	RAATTY	1 cut(s)	132
AspA2I	CCTAGG	1 cut(s)	122
AspLEI	GCGC	1 cut(s)	1451
AspS9I	GGNCC	3 cut(s)	126, 170, 184
AsuC2I	CCSGG	3 cut(s)	168, 188, 197
AsuHPI	GGTGA	4 cut(s)	9, 73, 823, 1383
AvaI	CYCGRG	1 cut(s)	1304
AvaII	GGWCC	3 cut(s)	126, 170, 184
AvrII	CCTAGG	1 cut(s)	122
BaeI	ACNNNNGTAYC	2 cut(s)	486, 519
BanI	GGYRCC	1 cut(s)	483
BbsI	GAAGAC	2 cut(s)	257, 326
BccI	CCATC	5 cut(s)	351, 439, 877, 1328, 1439
BceAI	ACGGC	1 cut(s)	226
BcgI	CGANNNNNNTGC	2 cut(s)	120, 154
BciT130I	CCWGG	2 cut(s)	1395, 1404
BciVI	GTATCC	1 cut(s)	566
BclI	TGATCA	1 cut(s)	951
BcnI	CCSGG	3 cut(s)	168, 188, 197
BfaI	CTAG	3 cut(s)	123, 1095, 1364
BfuI	GTATCC	1 cut(s)	566
BlnI	CCTAGG	1 cut(s)	122
Bme1390I	CCNGG	5 cut(s)	168, 188, 197, 1395, 1404
Bme18I	GGWCC	3 cut(s)	126, 170, 184
BmeT110I	CYCGRG	1 cut(s)	1304
BmgT120I	GGNCC	3 cut(s)	126, 170, 184
BmiI	GGNNCC	5 cut(s)	128, 171, 186, 485, 1408
BmrFI	CCNGG	5 cut(s)	168, 188, 197, 1395, 1404
BmsI	GCATC	6 cut(s)	109, 351, 459, 593, 736, 1149
BpiI	GAAGAC	2 cut(s)	257, 326
BpuMI	CCSGG	3 cut(s)	168, 188, 197
BsaAI	YACGTR	1 cut(s)	729
BsaJI	CCNNGG	8 cut(s)	89, 122, 180, 537, 915, 1191, 1323, 1394
BsaXI	ACNNNNNCTCC	4 cut(s)	214, 244, 518, 548
Bsc4I	CCNNNNNNNGG	2 cut(s)	90, 202
Bse118I	RCCGGY	1 cut(s)	486
Bse3DI	GCAATG	2 cut(s)	289, 456
BseBI	CCWGG	2 cut(s)	1395, 1404
BseDI	CCNNGG	8 cut(s)	89, 122, 180, 537, 915, 1191, 1323, 1394
BseGI	GGATG	7 cut(s)	100, 366, 450, 751, 804, 1105, 1450
BseLI	CCNNNNNNNGG	2 cut(s)	90, 202
BseMI	GCAATG	2 cut(s)	289, 456
BseMII	CTCAG	2 cut(s)	456, 534
BseRI	GAGGAG	1 cut(s)	235
BseYI	CCCAGC	2 cut(s)	84, 611
BshFI	GGCC	2 cut(s)	435, 490
BshNI	GGYRCC	1 cut(s)	483
BsiHKCI	CYCGRG	1 cut(s)	1304
BsiSI	CCGG	4 cut(s)	167, 188, 196, 487
BslFI	GGGAC	2 cut(s)	183, 1328
BslI	CCNNNNNNNGG	2 cut(s)	90, 202
BsmFI	GGGAC	2 cut(s)	183, 1328
BsmI	GAATGC	1 cut(s)	1159
BsnI	GGCC	2 cut(s)	435, 490
BsoBI	CYCGRG	1 cut(s)	1304
Bsp143I	GATC	3 cut(s)	411, 691, 951
Bsp19I	CCATGG	3 cut(s)	89, 1191, 1323
BspACI	CCGC	4 cut(s)	98, 104, 373, 989
BspANI	GGCC	2 cut(s)	435, 490
BspCNI	CTCAG	2 cut(s)	457, 533
BspHI	TCATGA	2 cut(s)	216, 1059
BspLI	GGNNCC	5 cut(s)	128, 171, 186, 485, 1408
BspPI	GGATC	1 cut(s)	699
BspQI	GCTCTTC	1 cut(s)	303
BspT107I	GGYRCC	1 cut(s)	483
BsrDI	GCAATG	2 cut(s)	289, 456
BsrFI	RCCGGY	1 cut(s)	486
BssAI	RCCGGY	1 cut(s)	486
BssECI	CCNNGG	8 cut(s)	89, 122, 180, 537, 915, 1191, 1323, 1394
BssMI	GATC	3 cut(s)	411, 691, 951
BssT1I	CCWWGG	6 cut(s)	89, 122, 537, 915, 1191, 1323
Bst2UI	CCWGG	2 cut(s)	1395, 1404
Bst4CI	ACNGT	1 cut(s)	395
Bst6I	CTCTTC	2 cut(s)	303, 1393
BstBAI	YACGTR	1 cut(s)	729
BstC8I	GCNNGC	1 cut(s)	488
BstDEI	CTNAG	4 cut(s)	465, 520, 1281, 1458
BstDSI	CCRYGG	3 cut(s)	89, 1191, 1323
BstF5I	GGATG	7 cut(s)	100, 366, 450, 751, 804, 1105, 1450
BstHHI	GCGC	1 cut(s)	1451
BstKTI	GATC	3 cut(s)	414, 694, 954
BstMBI	GATC	3 cut(s)	411, 691, 951
BstMWI	GCNNNNNNNGC	4 cut(s)	370, 612, 1376, 1424
BstNI	CCWGG	2 cut(s)	1395, 1404
BstNSI	RCATGY	1 cut(s)	998
BstSCI	CCNGG	5 cut(s)	166, 186, 195, 1393, 1402
BstSNI	TACGTA	1 cut(s)	729
BstV2I	GAAGAC	2 cut(s)	257, 326
BstX2I	RGATCY	1 cut(s)	691
BstYI	RGATCY	1 cut(s)	691
BsuI	GTATCC	1 cut(s)	566
BsuRI	GGCC	2 cut(s)	435, 490
BtgI	CCRYGG	3 cut(s)	89, 1191, 1323
BtgZI	GCGATG	1 cut(s)	1172
BtsCI	GGATG	7 cut(s)	100, 366, 450, 751, 804, 1105, 1450
BtsIMutI	CAGTG	4 cut(s)	235, 344, 818, 1222
Cac8I	GCNNGC	1 cut(s)	488
CciI	TCATGA	2 cut(s)	216, 1059
CfoI	GCGC	1 cut(s)	1451
Cfr10I	RCCGGY	1 cut(s)	486
Cfr13I	GGNCC	3 cut(s)	126, 170, 184
CseI	GACGC	2 cut(s)	220, 262
Csp6I	GTAC	4 cut(s)	265, 379, 625, 1235
CviAII	CATG	9 cut(s)	90, 217, 995, 1018, 1060, 1177, 1192, 1291, 1324
CviQI	GTAC	4 cut(s)	265, 379, 625, 1235
DdeI	CTNAG	4 cut(s)	465, 520, 1281, 1458
DpnI	GATC	3 cut(s)	413, 693, 953
DpnII	GATC	3 cut(s)	411, 691, 951
DrdI	GACNNNNNNGTC	1 cut(s)	191
DseDI	GACNNNNNNGTC	1 cut(s)	191
Eam1104I	CTCTTC	2 cut(s)	303, 1393
EarI	CTCTTC	2 cut(s)	303, 1393
Eco105I	TACGTA	1 cut(s)	729
Eco130I	CCWWGG	6 cut(s)	89, 122, 537, 915, 1191, 1323
Eco47I	GGWCC	3 cut(s)	126, 170, 184
Eco57I	CTGAAG	3 cut(s)	211, 320, 1337
Eco88I	CYCGRG	1 cut(s)	1304
EcoO109I	RGGNCCY	2 cut(s)	126, 184
EcoRII	CCWGG	2 cut(s)	1393, 1402
EcoT14I	CCWWGG	6 cut(s)	89, 122, 537, 915, 1191, 1323
EcoT22I	ATGCAT	1 cut(s)	751
ErhI	CCWWGG	6 cut(s)	89, 122, 537, 915, 1191, 1323
FaeI	CATG	9 cut(s)	93, 220, 998, 1021, 1063, 1180, 1195, 1294, 1327
FalI	AAGNNNNNCTT	2 cut(s)	1343, 1375
FaqI	GGGAC	2 cut(s)	183, 1328
FatI	CATG	9 cut(s)	89, 216, 994, 1017, 1059, 1176, 1191, 1290, 1323
FauNDI	CATATG	3 cut(s)	751, 856, 1045
FbaI	TGATCA	1 cut(s)	951
FblI	GTMKAC	1 cut(s)	504
FokI	GGATG	7 cut(s)	87, 373, 457, 758, 811, 1112, 1457
FspBI	CTAG	3 cut(s)	123, 1095, 1364
GlaI	GCGC	1 cut(s)	1450
GsaI	CCCAGC	2 cut(s)	88, 615
HaeIII	GGCC	2 cut(s)	435, 490
HapII	CCGG	4 cut(s)	167, 188, 196, 487
HgaI	GACGC	2 cut(s)	220, 262
HhaI	GCGC	1 cut(s)	1451
Hin1II	CATG	9 cut(s)	93, 220, 998, 1021, 1063, 1180, 1195, 1294, 1327
Hin6I	GCGC	1 cut(s)	1449
HinP1I	GCGC	1 cut(s)	1449
HindIII	AAGCTT	2 cut(s)	1033, 1205
HinfI	GANTC	5 cut(s)	782, 851, 1051, 1121, 1399
HpaII	CCGG	4 cut(s)	167, 188, 196, 487
HphI	GGTGA	4 cut(s)	9, 73, 823, 1383
Hpy166II	GTNNAC	2 cut(s)	505, 1171
Hpy188I	TCNGA	5 cut(s)	210, 356, 444, 466, 798
Hpy188III	TCNNGA	9 cut(s)	217, 657, 695, 1003, 1060, 1125, 1304, 1306, 1364
Hpy8I	GTNNAC	2 cut(s)	505, 1171
HpyAV	CCTTC	4 cut(s)	583, 961, 1122, 1288
HpyCH4III	ACNGT	1 cut(s)	395
HpyCH4IV	ACGT	5 cut(s)	267, 381, 623, 728, 938
HpyCH4V	TGCA	6 cut(s)	364, 512, 650, 749, 1176, 1220
HpyF10VI	GCNNNNNNNGC	4 cut(s)	370, 612, 1376, 1424
HpyF3I	CTNAG	4 cut(s)	465, 520, 1281, 1458
HpySE526I	ACGT	5 cut(s)	267, 381, 623, 728, 938
Hsp92II	CATG	9 cut(s)	93, 220, 998, 1021, 1063, 1180, 1195, 1294, 1327
HspAI	GCGC	1 cut(s)	1449
KroI	GCCGGC	1 cut(s)	486
KroNI	GCCGGC	1 cut(s)	488
Ksp22I	TGATCA	1 cut(s)	951
Kzo9I	GATC	3 cut(s)	411, 691, 951
LguI	GCTCTTC	1 cut(s)	303
LmnI	GCTCC	5 cut(s)	222, 286, 1132, 1297, 1412
LweI	GCATC	6 cut(s)	109, 351, 459, 593, 736, 1149
MaeI	CTAG	3 cut(s)	123, 1095, 1364
MaeII	ACGT	5 cut(s)	267, 381, 623, 728, 938
MaeIII	GTNAC	3 cut(s)	230, 619, 1411
MalI	GATC	3 cut(s)	413, 693, 953
MboI	GATC	3 cut(s)	411, 691, 951
MboII	GAAGA	7 cut(s)	262, 320, 331, 769, 874, 1018, 1380
MflI	RGATCY	1 cut(s)	691
MluCI	AATT	8 cut(s)	47, 70, 132, 407, 838, 872, 943, 958
MlyI	GAGTC	2 cut(s)	1060, 1408
MmeI	TCCRAC	2 cut(s)	55, 959
MnlI	CCTC	9 cut(s)	46, 73, 175, 193, 213, 273, 340, 1347, 1360
Mph1103I	ATGCAT	1 cut(s)	751
MroNI	GCCGGC	1 cut(s)	486
MseI	TTAA	6 cut(s)	270, 530, 687, 837, 897, 942
MspA1I	CMGCKG	1 cut(s)	373
MspI	CCGG	4 cut(s)	167, 188, 196, 487
MspR9I	CCNGG	5 cut(s)	168, 188, 197, 1395, 1404
Mva1269I	GAATGC	1 cut(s)	1159
MvaI	CCWGG	2 cut(s)	1395, 1404
MwoI	GCNNNNNNNGC	4 cut(s)	370, 612, 1376, 1424
NaeI	GCCGGC	1 cut(s)	488
NciI	CCSGG	3 cut(s)	168, 188, 197
NcoI	CCATGG	3 cut(s)	89, 1191, 1323
NdeI	CATATG	3 cut(s)	751, 856, 1045
NdeII	GATC	3 cut(s)	411, 691, 951
NgoMIV	GCCGGC	1 cut(s)	486
NlaIII	CATG	9 cut(s)	93, 220, 998, 1021, 1063, 1180, 1195, 1294, 1327
NlaIV	GGNNCC	5 cut(s)	128, 171, 186, 485, 1408
NmuCI	GTSAC	3 cut(s)	230, 619, 1411
NsiI	ATGCAT	1 cut(s)	751
NspI	RCATGY	1 cut(s)	998
PaeR7I	CTCGAG	1 cut(s)	1304
PagI	TCATGA	2 cut(s)	216, 1059
PciI	ACATGT	1 cut(s)	994
PciSI	GCTCTTC	1 cut(s)	303
PcsI	WCGNNNNNNNCGW	1 cut(s)	1154
PctI	GAATGC	1 cut(s)	1159
PdiI	GCCGGC	1 cut(s)	488
PfeI	GAWTC	3 cut(s)	782, 851, 1121
PfoI	TCCNGGA	2 cut(s)	166, 195
PleI	GAGTC	2 cut(s)	1059, 1407
PpsI	GAGTC	2 cut(s)	1059, 1407
Ppu21I	YACGTR	1 cut(s)	729
PpuMI	RGGWCCY	2 cut(s)	126, 184
PscI	ACATGT	1 cut(s)	994
Psp1406I	AACGTT	1 cut(s)	938
Psp5II	RGGWCCY	2 cut(s)	126, 184
Psp6I	CCWGG	2 cut(s)	1393, 1402
PspFI	CCCAGC	2 cut(s)	84, 611
PspGI	CCWGG	2 cut(s)	1393, 1402
PspN4I	GGNNCC	5 cut(s)	128, 171, 186, 485, 1408
PspPI	GGNCC	3 cut(s)	126, 170, 184
PspPPI	RGGWCCY	2 cut(s)	126, 184
PsuI	RGATCY	1 cut(s)	691
RsaI	GTAC	4 cut(s)	266, 380, 626, 1236
RsaNI	GTAC	4 cut(s)	265, 379, 625, 1235
SapI	GCTCTTC	1 cut(s)	303
SaqAI	TTAA	6 cut(s)	270, 530, 687, 837, 897, 942
Sau3AI	GATC	3 cut(s)	411, 691, 951
Sau96I	GGNCC	3 cut(s)	126, 170, 184
SchI	GAGTC	2 cut(s)	1060, 1408
ScrFI	CCNGG	5 cut(s)	168, 188, 197, 1395, 1404
SfaNI	GCATC	6 cut(s)	109, 351, 459, 593, 736, 1149
Sfr274I	CTCGAG	1 cut(s)	1304
SinI	GGWCC	3 cut(s)	126, 170, 184
SlaI	CTCGAG	1 cut(s)	1304
SmlI	CTYRAG	1 cut(s)	1304
SmoI	CTYRAG	1 cut(s)	1304
SnaBI	TACGTA	1 cut(s)	729
Sse9I	AATT	8 cut(s)	47, 70, 132, 407, 838, 872, 943, 958
SsiI	CCGC	4 cut(s)	98, 104, 373, 989
SspMI	CTAG	3 cut(s)	123, 1095, 1364
StyD4I	CCNGG	5 cut(s)	166, 186, 195, 1393, 1402
StyI	CCWWGG	6 cut(s)	89, 122, 537, 915, 1191, 1323
TaaI	ACNGT	1 cut(s)	395
TaiI	ACGT	5 cut(s)	270, 384, 626, 731, 941
TaqI	TCGA	4 cut(s)	146, 658, 876, 1305
TaqII	GACCGA	1 cut(s)	194
TasI	AATT	8 cut(s)	47, 70, 132, 407, 838, 872, 943, 958
TfiI	GAWTC	3 cut(s)	782, 851, 1121
Tru1I	TTAA	6 cut(s)	270, 530, 687, 837, 897, 942
Tru9I	TTAA	6 cut(s)	270, 530, 687, 837, 897, 942
TscAI	CASTG	4 cut(s)	235, 344, 818, 1222
TseFI	GTSAC	3 cut(s)	230, 619, 1411
Tsp45I	GTSAC	3 cut(s)	230, 619, 1411
TspDTI	ATGAA	8 cut(s)	47, 602, 815, 843, 1027, 1034, 1193, 1307
TspGWI	ACGGA	3 cut(s)	101, 299, 1399
TspRI	CASTG	4 cut(s)	235, 344, 818, 1222
VpaK11BI	GGWCC	3 cut(s)	126, 170, 184
XapI	RAATTY	1 cut(s)	132
XbaI	TCTAGA	1 cut(s)	1363
XceI	RCATGY	1 cut(s)	998
XhoI	CTCGAG	1 cut(s)	1304
XmaJI	CCTAGG	1 cut(s)	122
XmiI	GTMKAC	1 cut(s)	504
XspI	CTAG	3 cut(s)	123, 1095, 1364
Zsp2I	ATGCAT	1 cut(s)	751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.