MD03G1189100.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014696
Physical Location & Seq
Forward (+)
25963749 .. 25964348
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1189100.v1.1.491

Sequence Viewer

Length: 600 bp
ATGCGTAAATGGAAGGGTGTGACATGCAGCTCTCACTCGACTTCGATAACAGAAATCAGGCTTGAAAATCTGAATCTTAGTGGCATACTTGATGCAGATTCACTATGTAAGCTCCCAGTTGTTAATTTAGCAAGAAACCAAATTGGGGGAACTATTTCTAAATCAATATTGCATTGCACAAGGCTAACCTATTTGAATCTAAGCAGCAATATTCTGAGTGGGAGGATACCTAAGGCTCTGACTAAACTGAGATATTTGAGGAGATTGGACGTCTCTAACAATCATTTTACCAGCACTGCCTCACGCTTTGAACAGGGATTTCAGCCGGTTCATACATACGTGAAGAAATCGAGGATATTACAAAGATATGGTGCCCAGAAGCAGGAAAAGGTAGTAGATTCGATGGCCATAGTGACAGAGTCCGAGAGCCCGAATGGTGGCAGTGACAGTGTTCCACGTCCCTCTACACCATGGTACAAGAGATACAAATGCATGGTACCATTAGTTGTGGGGCTTGGCTTCTTTCTCTTATTCATGTACTTTGTAGGAAGGCAGGCTGCTAAGTTAGCTGAAGACAGGGAGATTCTGAAGTCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

200

Amino Acids

22.41

Weight (kDa)

9.98

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 38 - 96 1.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014696)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23970
malus_domestica MD03G1189100.v1.1 MD11G1205400.v1.1
prunus_persica Prupe.4G232000_v2.0.a1
pyrus_communis pycom11g17810
rosa_chinensis RchiOBHm_Chr5g0042511
rosa_laevigata RLG00000034134
rosa_multiflora Rmu_co8185866.1_g000001 Rmu_sc0009319.1_g000001 Rmu_sc0041109.1_g000002
rosa_roxburghii Rroxscaffold_1G00038130
rosa_rugosa Rorug05G0201200
rosa_samantha Rh5AG287100 Rh5BG291500 Rh5CG323800 Rh5DG301300
rosa_wichuraiana Rw5G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
Acc65I GGTACC 1 cut(s) 496
AccB1I GGYRCC 2 cut(s) 371, 496
AcoI YGGCCR 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 591
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 3 cut(s) 476, 498, 539
AfiI CCNNNNNNNGG 3 cut(s) 145, 382, 437
AgsI TTSAA 3 cut(s) 65, 196, 311
AjiI CACGTC 1 cut(s) 458
AluBI AGCT 3 cut(s) 30, 112, 569
AluI AGCT 3 cut(s) 30, 112, 569
Alw26I GTCTC 1 cut(s) 277
AoxI GGCC 1 cut(s) 405
ApeKI GCWGC 3 cut(s) 27, 204, 557
Asp700I GAANNNNTTC 1 cut(s) 154
Asp718I GGTACC 1 cut(s) 496
AxyI CCTNAGG 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 407
BanI GGYRCC 2 cut(s) 371, 496
BanII GRGCYC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 579
BbvI GCAGC 3 cut(s) 39, 216, 544
BccI CCATC 1 cut(s) 397
BciVI GTATCC 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 277
BfuI GTATCC 1 cut(s) 219
BisI GCNGC 3 cut(s) 28, 205, 558
BlsI GCNGC 3 cut(s) 29, 206, 559
BmgBI CACGTC 1 cut(s) 458
BmiI GGNNCC 2 cut(s) 373, 498
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 1 cut(s) 82
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 579
BsaAI YACGTR 1 cut(s) 340
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 470
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 382, 437
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 116
Bse21I CCTNAGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 470
BseLI CCNNNNNNNGG 3 cut(s) 145, 382, 437
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 2 cut(s) 206, 239
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 274
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 3 cut(s) 39, 216, 544
BshFI GGCC 1 cut(s) 407
BshNI GGYRCC 2 cut(s) 371, 496
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 444
BslI CCNNNNNNNGG 3 cut(s) 145, 382, 437
BsmAI GTCTC 1 cut(s) 277
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 444
BsnI GGCC 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 376, 431
Bsp19I CCATGG 1 cut(s) 470
BspANI GGCC 1 cut(s) 407
BspCNI CTCAG 2 cut(s) 207, 240
BspLI GGNNCC 2 cut(s) 373, 498
BspT107I GGYRCC 2 cut(s) 371, 496
BsrDI GCAATG 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 470
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 470
Bst4CI ACNGT 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 270
BstBAI YACGTR 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 6 cut(s) 77, 200, 215, 231, 248, 561
BstDSI CCRYGG 1 cut(s) 470
BstMAI GTCTC 1 cut(s) 277
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNSI RCATGY 1 cut(s) 27
BstSLI GKGCMC 1 cut(s) 376
BstV1I GCAGC 3 cut(s) 39, 216, 544
BstV2I GAAGAC 1 cut(s) 579
Bsu36I CCTNAGG 1 cut(s) 231
BsuI GTATCC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 407
BtgI CCRYGG 1 cut(s) 470
BtrI CACGTC 1 cut(s) 458
BtsI GCAGTG 2 cut(s) 294, 448
BtsIMutI CAGTG 3 cut(s) 294, 448, 454
Cac8I GCNNGC 1 cut(s) 555
Cfr10I RCCGGY 1 cut(s) 325
Csp6I GTAC 3 cut(s) 475, 497, 538
CviAII CATG 4 cut(s) 24, 471, 493, 535
CviQI GTAC 3 cut(s) 475, 497, 538
DdeI CTNAG 6 cut(s) 77, 200, 215, 231, 248, 561
EaeI YGGCCR 1 cut(s) 405
Eco130I CCWWGG 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 470
EcoT22I ATGCAT 1 cut(s) 494
EcoT38I GRGCYC 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 470
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 4 cut(s) 27, 474, 496, 538
FaqI GGGAC 1 cut(s) 444
FatI CATG 4 cut(s) 23, 470, 492, 534
Fnu4HI GCNGC 3 cut(s) 28, 205, 558
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 3 cut(s) 28, 205, 558
GluI GCNGC 3 cut(s) 28, 205, 558
HaeIII GGCC 1 cut(s) 407
HapII CCGG 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 4 cut(s) 27, 474, 496, 538
HinfI GANTC 6 cut(s) 73, 98, 196, 398, 419, 583
HpaII CCGG 1 cut(s) 326
Hpy188I TCNGA 5 cut(s) 72, 216, 240, 424, 588
HpyAV CCTTC 2 cut(s) 7, 543
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4IV ACGT 3 cut(s) 270, 339, 457
HpyCH4V TGCA 5 cut(s) 27, 95, 172, 177, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 6 cut(s) 77, 200, 215, 231, 248, 561
HpySE526I ACGT 3 cut(s) 270, 339, 457
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 4 cut(s) 27, 474, 496, 538
KpnI GGTACC 1 cut(s) 500
LmnI GCTCC 1 cut(s) 117
LpnPI CCDG 9 cut(s) 43, 129, 299, 304, 339, 368, 389, 539, 562
Lsp1109I GCAGC 3 cut(s) 39, 216, 544
LweI GCATC 1 cut(s) 82
MaeII ACGT 3 cut(s) 270, 339, 457
MaeIII GTNAC 3 cut(s) 19, 412, 443
MboII GAAGA 2 cut(s) 355, 584
MhlI GDGCHC 2 cut(s) 376, 431
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 2 cut(s) 124, 141
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 5 cut(s) 216, 252, 310, 345, 472
Mox20I TGGCCA 1 cut(s) 407
Mph1103I ATGCAT 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 154
MscI TGGCCA 1 cut(s) 407
MseI TTAA 1 cut(s) 123
Msp20I TGGCCA 1 cut(s) 407
MspI CCGG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 566
NcoI CCATGG 1 cut(s) 470
NlaIII CATG 4 cut(s) 27, 474, 496, 538
NlaIV GGNNCC 2 cut(s) 373, 498
NmuCI GTSAC 3 cut(s) 19, 412, 443
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 154
PfeI GAWTC 5 cut(s) 73, 98, 196, 398, 583
PflFI GACNNNGTC 1 cut(s) 418
PkrI GCNGC 3 cut(s) 29, 206, 559
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Ppu21I YACGTR 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 373, 498
PsyI GACNNNGTC 1 cut(s) 418
RsaI GTAC 3 cut(s) 476, 498, 539
RsaNI GTAC 3 cut(s) 475, 497, 538
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 3 cut(s) 28, 205, 558
SchI GAGTC 1 cut(s) 428
SduI GDGCHC 2 cut(s) 376, 431
SetI ASST 9 cut(s) 32, 114, 191, 232, 273, 342, 393, 460, 571
SfaNI GCATC 1 cut(s) 82
Sse9I AATT 2 cut(s) 124, 141
SspI AATATT 2 cut(s) 168, 211
StyI CCWWGG 1 cut(s) 470
TaaI ACNGT 1 cut(s) 449
TaiI ACGT 3 cut(s) 273, 342, 460
TaqI TCGA 4 cut(s) 38, 44, 350, 401
TasI AATT 2 cut(s) 124, 141
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 5 cut(s) 73, 98, 196, 398, 583
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 3 cut(s) 301, 448, 454
TseFI GTSAC 3 cut(s) 19, 412, 443
TseI GCWGC 3 cut(s) 27, 204, 557
Tsp45I GTSAC 3 cut(s) 19, 412, 443
TspDTI ATGAA 2 cut(s) 320, 523
TspRI CASTG 3 cut(s) 301, 448, 454
Tth111I GACNNNGTC 1 cut(s) 418
XceI RCATGY 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 154
ZraI GACGTC 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.