MD11G1205400.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014696
Physical Location & Seq
Forward (+)
30010108 .. 30012101
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1205400.v1.1.491

Sequence Viewer

Length: 1836 bp
ATGAGTCCAACAAAAATGCCGAGCAGGAGAGGTTTCAATCTGTTTCTGAAGGGACTGATATGCATTGGGGTGTTTTCTTTCTCAATCGCGGTATGCATGGGAGGCGAGTCACCAGAGTTTGAAGCTTTGCTGCAATTCATTAGAGCTGTTGATCCTCAAGATGTACTTAAAGTTGGCCAGAATGCACCTATGCGGCAAAATCCCTGCATACATAAGTGGAAGGGTGTGACATGCAACTCACTCGCTACTTCGATAACAGAAATCAGGCTTGAAAATTTGAATCTTAGTGGCATACTTGATGTAAGTTCACTATGTAATCTCCCGAATCTTCGAGTTGTTAGCCTAGCAAGAAACCAAATTGGGGGAACTATTTCTACCTCAATATTGCACTGCACAAGGCTAACATATTTGAATATAAGCAGCAATGTTCTGAGTGGAAGGATACCCAAGGCTCTGACTAAACTGAAATATCTGAGGAGACTGGACATCTCCAACAATCATTTTATTAGCACCGCCTCACGCTCTGAAGAGGGAGTTCAGCCGATTCATACATACGTGAAGAAATCGAGGAGATTACAGAGACATGGTGCCATGAAGCAGGAAAAGGTAGTAAATTCAGTGGCCGTAGAGACAGAGTCCGAGAGACCAAGCAGTGGCAGTGGAAGTGATTCAGATACATCTAGACCGTGGTACAAGAGATATGAATGCATGATGCCATTAATTGTGGGGCTTGGCTTCTTTCTCTTGTTCATGTACTTTGCGGGAAGGAAGGCTGCTAAGTTAGCTGAAGAGAGGGAGATTCTCAAATCGCTTCGAGACTCGCCTCAGAATATGCCGCCTCCTAAGGTTCAAGAGGGAGAGAAGCCAGTGGAGAACCGGTCGGAGCTCGTCTTCTTTGTTGAAGAACATGAAAGTTTTAAATTGGAAGACCTCCTTGAAGCCACAGCTGACTTGAGAAACCAGTCCTTTTGCAGCAGCCTTTACAAGGTAATACTGAAAAACAATGCCCTATATGCAGTCAAAAGATTGAAGAAATTGCAAGTACCCTTTGAGGAGTTTGGCCAAACAATGAGACAAATAGGGATTATAAAGCATCCCAACATTCTACCACTCGTTGGTTACAATTCCACTAATGAAGAAAAACTTTTGATCTACAAGTTTCAAAGCAATGGAAGTCTGCTAAACCTGCTTGAGAGCTACACAGAAGGTAAGAGGGATTTTCCATGGAGGCTTCGGCTCTCCATAGCACACGGTATTTCAAAGGGGCTAGACTTCATATATCAGAGGTCTGATGACTGCATCCCTCATGGGAATCTCAAGCTTTCAAATATCCTCTTAGATGACAATGATGAACCACTGATTAGCGAGTACGGTCTTTCAAGTTTCTTCAACCCCAAGAAAGGGTGTACCATTTCCTCCAATGGTTACACAGCCCCAGAGAAAATGTTATCAGAAAAGGGAGATGTTTATAGCTTCGGCGTGATTCTTCTGGAGTTACTAACAGGCAAAACTGTTGAAGGAACTGGGATAGACCTTCCTAAGTGGGTGACTGCCATGGTTAAGGAGGAGTGGACTGGGGAGGTCTTCGACAAGGAGGTTGCTAAAGCTGCAAAAGAATGGGCATTTCCTCTTCTTAACATTGCCCTCAAGTGTGTATCAGCTTCACCTACAAACAGGCCAACTGCGGAAAATGTTTACGGAAAGATTGAAGAGGTTATAAATGACACTCCAAATTCATCGGATTGTACTACAGAGTGTAGTGGCTACCAAGAAAACTGCTGTATGCTTCACTCCATCATACCTGAGACCTGGGACAGTCCTGGATCAAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

612

Amino Acids

68.47

Weight (kDa)

8.05

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 108 - 168 2e-08 Leucine rich repeat
Pkinase PF00069 326 - 565 1.2e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 334 - 566 1.9e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014696)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23970
malus_domestica MD03G1189100.v1.1 MD11G1205400.v1.1
prunus_persica Prupe.4G232000_v2.0.a1
pyrus_communis pycom11g17810
rosa_chinensis RchiOBHm_Chr5g0042511
rosa_laevigata RLG00000034134
rosa_multiflora Rmu_co8185866.1_g000001 Rmu_sc0009319.1_g000001 Rmu_sc0041109.1_g000002
rosa_roxburghii Rroxscaffold_1G00038130
rosa_rugosa Rorug05G0201200
rosa_samantha Rh5AG287100 Rh5BG291500 Rh5CG323800 Rh5DG301300
rosa_wichuraiana Rw5G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1088, 1718
Acc36I ACCTGC 1 cut(s) 1194
AccB1I GGYRCC 1 cut(s) 587
AccB7I CCANNNNNTGG 2 cut(s) 653, 1115
AccII CGCG 1 cut(s) 89
AciI CCGC 6 cut(s) 89, 193, 513, 761, 836, 1685
AclWI GGATC 2 cut(s) 146, 1831
AcoI YGGCCR 3 cut(s) 175, 621, 1060
AcsI RAATTY 3 cut(s) 274, 613, 1732
AcuI CTGAAG 3 cut(s) 68, 546, 807
AfaI GTAC 7 cut(s) 165, 692, 755, 1044, 1370, 1408, 1747
AfiI CCNNNNNNNGG 6 cut(s) 361, 653, 985, 1115, 1400, 1401
AgeI ACCGGT 1 cut(s) 876
AjnI CCWGG 2 cut(s) 1808, 1819
Alw21I GWGCWC 1 cut(s) 888
Alw26I GTCTC 7 cut(s) 472, 574, 623, 637, 810, 1066, 1799
AlwI GGATC 2 cut(s) 146, 1831
AoxI GGCC 4 cut(s) 175, 621, 1060, 1676
ApeKI GCWGC 6 cut(s) 130, 420, 773, 972, 975, 1607
ApoI RAATTY 3 cut(s) 274, 613, 1732
AseI ATTAAT 1 cut(s) 719
AsiGI ACCGGT 1 cut(s) 876
Asp700I GAANNNNTTC 2 cut(s) 370, 667
AsuHPI GGTGA 3 cut(s) 102, 1558, 1656
AxyI CCTNAGG 1 cut(s) 843
BalI TGGCCA 2 cut(s) 177, 1062
BanI GGYRCC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 888
BbsI GAAGAC 3 cut(s) 883, 933, 1576
Bbv12I GWGCWC 1 cut(s) 888
BbvI GCAGC 6 cut(s) 117, 432, 760, 984, 987, 1594
BccI CCATC 1 cut(s) 1802
BceAI ACGGC 1 cut(s) 608
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 2 cut(s) 1810, 1821
BciVI GTATCC 1 cut(s) 435
BcoDI GTCTC 7 cut(s) 472, 574, 623, 637, 810, 1066, 1799
BfaI CTAG 3 cut(s) 344, 681, 1268
BfmI CTRYAG 1 cut(s) 1749
BfuAI ACCTGC 1 cut(s) 1194
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 8 cut(s) 131, 194, 421, 774, 836, 973, 976, 1608
BlsI GCNGC 8 cut(s) 132, 195, 422, 775, 837, 974, 977, 1609
Bme1390I CCNGG 2 cut(s) 1810, 1821
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 2 cut(s) 1810, 1821
BmrI ACTGGG 2 cut(s) 1533, 1584
BmsI GCATC 3 cut(s) 702, 1102, 1308
BmuI ACTGGG 2 cut(s) 1533, 1584
BpiI GAAGAC 3 cut(s) 883, 933, 1576
BpmI CTGGAG 1 cut(s) 1511
BpuEI CTTGAG 5 cut(s) 141, 973, 1211, 1301, 1631
BsaAI YACGTR 1 cut(s) 556
BsaI GGTCTC 2 cut(s) 637, 1799
BsaJI CCNNGG 5 cut(s) 447, 686, 1223, 1554, 1809
BsaWI WCCGGW 1 cut(s) 876
BsaXI ACNNNNNCTCC 2 cut(s) 863, 893
Bsc4I CCNNNNNNNGG 6 cut(s) 361, 653, 985, 1115, 1400, 1401
Bse118I RCCGGY 1 cut(s) 876
Bse1I ACTGG 5 cut(s) 486, 866, 961, 1528, 1579
Bse21I CCTNAGG 1 cut(s) 843
Bse3DI GCAATG 3 cut(s) 430, 1174, 1638
BseBI CCWGG 2 cut(s) 1810, 1821
BseDI CCNNGG 5 cut(s) 447, 686, 1223, 1554, 1809
BseGI GGATG 2 cut(s) 1093, 1299
BseLI CCNNNNNNNGG 6 cut(s) 361, 653, 985, 1115, 1400, 1401
BseMI GCAATG 3 cut(s) 430, 1174, 1638
BseMII CTCAG 4 cut(s) 422, 464, 839, 1794
BseNI ACTGG 5 cut(s) 486, 866, 961, 1528, 1579
BseRI GAGGAG 4 cut(s) 490, 583, 1067, 1580
BseXI GCAGC 6 cut(s) 117, 432, 760, 984, 987, 1594
BsgI GTGCAG 1 cut(s) 376
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 881
BshFI GGCC 4 cut(s) 177, 623, 1062, 1678
BshNI GGYRCC 1 cut(s) 587
BshTI ACCGGT 1 cut(s) 876
BsiEI CGRYCG 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 888
BsiSI CCGG 1 cut(s) 877
BslFI GGGAC 2 cut(s) 66, 1826
BslI CCNNNNNNNGG 6 cut(s) 361, 653, 985, 1115, 1400, 1401
BsmAI GTCTC 7 cut(s) 472, 574, 623, 637, 810, 1066, 1799
BsmFI GGGAC 2 cut(s) 66, 1826
BsmI GAATGC 2 cut(s) 187, 710
BsnI GGCC 4 cut(s) 177, 623, 1062, 1678
Bso31I GGTCTC 2 cut(s) 637, 1799
Bsp1286I GDGCHC 1 cut(s) 888
Bsp143I GATC 3 cut(s) 151, 1149, 1823
Bsp19I CCATGG 2 cut(s) 1223, 1554
BspACI CCGC 6 cut(s) 89, 193, 513, 761, 836, 1685
BspANI GGCC 4 cut(s) 177, 623, 1062, 1678
BspCNI CTCAG 4 cut(s) 423, 465, 838, 1795
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 589
BspMI ACCTGC 1 cut(s) 1194
BspPI GGATC 2 cut(s) 146, 1831
BspT107I GGYRCC 1 cut(s) 587
BspTNI GGTCTC 2 cut(s) 637, 1799
BsrDI GCAATG 3 cut(s) 430, 1174, 1638
BsrFI RCCGGY 1 cut(s) 876
BsrI ACTGG 5 cut(s) 486, 866, 961, 1528, 1579
BssAI RCCGGY 1 cut(s) 876
BssECI CCNNGG 5 cut(s) 447, 686, 1223, 1554, 1809
BssMI GATC 3 cut(s) 151, 1149, 1823
BssT1I CCWWGG 3 cut(s) 447, 1223, 1554
Bst2UI CCWGG 2 cut(s) 1810, 1821
Bst4CI ACNGT 5 cut(s) 687, 1253, 1373, 1513, 1817
Bst6I CTCTTC 4 cut(s) 522, 783, 1635, 1704
BstBAI YACGTR 1 cut(s) 556
BstDEI CTNAG 9 cut(s) 284, 431, 473, 777, 825, 843, 1336, 1539, 1803
BstDSI CCRYGG 3 cut(s) 686, 1223, 1554
BstENI CCTNNNNNAGG 1 cut(s) 983
BstF5I GGATG 2 cut(s) 1093, 1299
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 3 cut(s) 154, 1152, 1826
BstMAI GTCTC 7 cut(s) 472, 574, 623, 637, 810, 1066, 1799
BstMBI GATC 3 cut(s) 151, 1149, 1823
BstMCI CGRYCG 1 cut(s) 881
BstMWI GCNNNNNNNGC 5 cut(s) 102, 782, 1013, 1186, 1607
BstNI CCWGG 2 cut(s) 1810, 1821
BstNSI RCATGY 1 cut(s) 234
BstSCI CCNGG 2 cut(s) 1808, 1819
BstSFI CTRYAG 1 cut(s) 1749
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 6 cut(s) 117, 432, 760, 984, 987, 1594
BstV2I GAAGAC 3 cut(s) 883, 933, 1576
Bsu36I CCTNAGG 1 cut(s) 843
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 4 cut(s) 177, 623, 1062, 1678
BtgI CCRYGG 3 cut(s) 686, 1223, 1554
BtsCI GGATG 2 cut(s) 1093, 1299
BtsI GCAGTG 3 cut(s) 388, 658, 664
BtsIMutI CAGTG 6 cut(s) 388, 624, 658, 664, 873, 1355
BveI ACCTGC 1 cut(s) 1194
Cfr10I RCCGGY 1 cut(s) 876
Csp6I GTAC 7 cut(s) 164, 691, 754, 1043, 1369, 1407, 1746
CspAI ACCGGT 1 cut(s) 876
CviQI GTAC 7 cut(s) 164, 691, 754, 1043, 1369, 1407, 1746
DdeI CTNAG 9 cut(s) 284, 431, 473, 777, 825, 843, 1336, 1539, 1803
DpnI GATC 3 cut(s) 153, 1151, 1825
DpnII GATC 3 cut(s) 151, 1149, 1823
DraI TTTAAA 1 cut(s) 919
EaeI YGGCCR 3 cut(s) 175, 621, 1060
Eam1104I CTCTTC 4 cut(s) 522, 783, 1635, 1704
EarI CTCTTC 4 cut(s) 522, 783, 1635, 1704
Ecl136II GAGCTC 1 cut(s) 886
Eco130I CCWWGG 3 cut(s) 447, 1223, 1554
Eco24I GRGCYC 1 cut(s) 888
Eco31I GGTCTC 2 cut(s) 637, 1799
Eco53kI GAGCTC 1 cut(s) 886
Eco57I CTGAAG 3 cut(s) 68, 546, 807
Eco81I CCTNAGG 1 cut(s) 843
EcoICRI GAGCTC 1 cut(s) 886
EcoNI CCTNNNNNAGG 1 cut(s) 983
EcoRII CCWGG 2 cut(s) 1808, 1819
EcoT14I CCWWGG 3 cut(s) 447, 1223, 1554
EcoT22I ATGCAT 3 cut(s) 65, 98, 710
EcoT38I GRGCYC 1 cut(s) 888
ErhI CCWWGG 3 cut(s) 447, 1223, 1554
FalI AAGNNNNNCTT 6 cut(s) 150, 182, 918, 950, 1128, 1160
FaqI GGGAC 2 cut(s) 66, 1826
FauI CCCGC 1 cut(s) 754
Fnu4HI GCNGC 8 cut(s) 131, 194, 421, 774, 836, 973, 976, 1608
FokI GGATG 2 cut(s) 1080, 1286
FriOI GRGCYC 1 cut(s) 888
Fsp4HI GCNGC 8 cut(s) 131, 194, 421, 774, 836, 973, 976, 1608
FspBI CTAG 3 cut(s) 344, 681, 1268
GluI GCNGC 8 cut(s) 131, 194, 421, 774, 836, 973, 976, 1608
GsuI CTGGAG 1 cut(s) 1511
HaeIII GGCC 4 cut(s) 177, 623, 1062, 1678
HapII CCGG 1 cut(s) 877
HindIII AAGCTT 2 cut(s) 123, 1319
HpaII CCGG 1 cut(s) 877
HphI GGTGA 3 cut(s) 102, 1558, 1656
Hpy166II GTNNAC 4 cut(s) 308, 1407, 1572, 1696
Hpy188III TCNNGA 6 cut(s) 158, 322, 681, 815, 851, 1490
Hpy8I GTNNAC 4 cut(s) 308, 1407, 1572, 1696
HpyAV CCTTC 8 cut(s) 43, 214, 432, 759, 763, 1199, 1511, 1544
HpyCH4III ACNGT 5 cut(s) 687, 1253, 1373, 1513, 1817
HpyCH4IV ACGT 1 cut(s) 555
HpyF10VI GCNNNNNNNGC 5 cut(s) 102, 782, 1013, 1186, 1607
HpyF3I CTNAG 9 cut(s) 284, 431, 473, 777, 825, 843, 1336, 1539, 1803
HpySE526I ACGT 1 cut(s) 555
Kzo9I GATC 3 cut(s) 151, 1149, 1823
LmnI GCTCC 1 cut(s) 883
Lsp1109I GCAGC 6 cut(s) 117, 432, 760, 984, 987, 1594
LweI GCATC 3 cut(s) 702, 1102, 1308
MaeI CTAG 3 cut(s) 344, 681, 1268
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 6 cut(s) 108, 226, 1118, 1424, 1494, 1546
MalI GATC 3 cut(s) 153, 1151, 1825
MboI GATC 3 cut(s) 151, 1149, 1823
MhlI GDGCHC 1 cut(s) 888
MlsI TGGCCA 2 cut(s) 177, 1062
MluNI TGGCCA 2 cut(s) 177, 1062
MlyI GAGTC 4 cut(s) 13, 116, 644, 812
MmeI TCCRAC 3 cut(s) 32, 516, 861
Mox20I TGGCCA 2 cut(s) 177, 1062
Mph1103I ATGCAT 3 cut(s) 65, 98, 710
MroXI GAANNNNTTC 2 cut(s) 370, 667
MscI TGGCCA 2 cut(s) 177, 1062
MseI TTAA 6 cut(s) 168, 719, 918, 1560, 1635, 1834
MslI CAYNNNNRTG 1 cut(s) 68
Msp20I TGGCCA 2 cut(s) 177, 1062
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 1 cut(s) 877
MspR9I CCNGG 2 cut(s) 1810, 1821
Mva1269I GAATGC 2 cut(s) 187, 710
MvaI CCWGG 2 cut(s) 1810, 1821
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 5 cut(s) 102, 782, 1013, 1186, 1607
NcoI CCATGG 2 cut(s) 1223, 1554
NdeII GATC 3 cut(s) 151, 1149, 1823
NlaIV GGNNCC 1 cut(s) 589
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 3 cut(s) 108, 226, 1546
NsiI ATGCAT 3 cut(s) 65, 98, 710
NspI RCATGY 1 cut(s) 234
PctI GAATGC 2 cut(s) 187, 710
PdmI GAANNNNTTC 2 cut(s) 370, 667
PfeI GAWTC 7 cut(s) 280, 325, 544, 668, 799, 1312, 1483
PflFI GACNNNGTC 1 cut(s) 634
PflMI CCANNNNNTGG 2 cut(s) 653, 1115
PfoI TCCNGGA 1 cut(s) 1819
PinAI ACCGGT 1 cut(s) 876
PkrI GCNGC 8 cut(s) 132, 195, 422, 775, 837, 974, 977, 1609
PleI GAGTC 4 cut(s) 12, 115, 643, 812
PpsI GAGTC 4 cut(s) 12, 115, 643, 812
Ppu21I YACGTR 1 cut(s) 556
PshBI ATTAAT 1 cut(s) 719
PsiI TTATAA 2 cut(s) 1088, 1718
Psp124BI GAGCTC 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 1808, 1819
PspGI CCWGG 2 cut(s) 1808, 1819
PspN4I GGNNCC 1 cut(s) 589
PsrI GAACNNNNNNTAC 2 cut(s) 358, 390
PsyI GACNNNGTC 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 7 cut(s) 165, 692, 755, 1044, 1370, 1408, 1747
RsaNI GTAC 7 cut(s) 164, 691, 754, 1043, 1369, 1407, 1746
RseI CAYNNNNRTG 1 cut(s) 68
SacI GAGCTC 1 cut(s) 888
SaqAI TTAA 6 cut(s) 168, 719, 918, 1560, 1635, 1834
SatI GCNGC 8 cut(s) 131, 194, 421, 774, 836, 973, 976, 1608
Sau3AI GATC 3 cut(s) 151, 1149, 1823
SchI GAGTC 4 cut(s) 13, 116, 644, 812
ScrFI CCNGG 2 cut(s) 1810, 1821
SduI GDGCHC 1 cut(s) 888
SfaNI GCATC 3 cut(s) 702, 1102, 1308
SfcI CTRYAG 1 cut(s) 1749
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 5 cut(s) 156, 952, 1190, 1316, 1646
SmoI CTYRAG 5 cut(s) 156, 952, 1190, 1316, 1646
SsiI CCGC 6 cut(s) 89, 193, 513, 761, 836, 1685
SspI AATATT 1 cut(s) 384
SspMI CTAG 3 cut(s) 344, 681, 1268
SstI GAGCTC 1 cut(s) 888
StyD4I CCNGG 2 cut(s) 1808, 1819
StyI CCWWGG 3 cut(s) 447, 1223, 1554
TaaI ACNGT 5 cut(s) 687, 1253, 1373, 1513, 1817
TaiI ACGT 1 cut(s) 558
TaqI TCGA 5 cut(s) 251, 331, 566, 814, 1587
TatI WGTACW 3 cut(s) 163, 753, 1745
TauI GCSGC 2 cut(s) 196, 838
TfiI GAWTC 7 cut(s) 280, 325, 544, 668, 799, 1312, 1483
Tru1I TTAA 6 cut(s) 168, 719, 918, 1560, 1635, 1834
Tru9I TTAA 6 cut(s) 168, 719, 918, 1560, 1635, 1834
TscAI CASTG 6 cut(s) 395, 624, 658, 664, 873, 1362
TseFI GTSAC 3 cut(s) 108, 226, 1546
TseI GCWGC 6 cut(s) 130, 420, 773, 972, 975, 1607
Tsp45I GTSAC 3 cut(s) 108, 226, 1546
TspGWI ACGGA 1 cut(s) 1713
TspRI CASTG 6 cut(s) 395, 624, 658, 664, 873, 1362
Tth111I GACNNNGTC 1 cut(s) 634
Van91I CCANNNNNTGG 2 cut(s) 653, 1115
VspI ATTAAT 1 cut(s) 719
XagI CCTNNNNNAGG 1 cut(s) 983
XapI RAATTY 3 cut(s) 274, 613, 1732
XbaI TCTAGA 1 cut(s) 680
XceI RCATGY 1 cut(s) 234
XmnI GAANNNNTTC 2 cut(s) 370, 667
XspI CTAG 3 cut(s) 344, 681, 1268
Zsp2I ATGCAT 3 cut(s) 65, 98, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.