MD03G1222500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015239
Physical Location & Seq
Reverse (-)
30676958 .. 30677290
333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1222500.v1.1.491

Sequence Viewer

Length: 333 bp
ATGCAAAAGAACAACCCTCACTACAGGTACATTGGAGAGAGGCTACAAACACTCACTGCTCAAAACAAGGGTTGTATGGTAGCGCCACCCTCATCATCATCATCCCCAAGGCCTCGGAAACAGGTATGTACGTGCTCGAATCGTCCCGGATTGAACAGGTGTAGCCGGCACGGATATGCAATGCCTCCGGGTGGCGAAAAGTGGAGGAGGAACTATGCAAACAAGGAGGTTTTGAGGAGGGCGTTGAGACATACACCAAATCGTAGCTTAAGTCTTCGGTGGTGGAATTTCCAACCGATGCCCAGTCGGCTTTCTAACATGTCAATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.74

Weight (kDa)

11.45

Isoelectric Point (pI)

95.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15518
fragaria_vesca FvH4_3g20710
malus_domestica MD03G1222500.v1.1 MD11G1239800.v1.1
prunus_persica Prupe.4G187200_v2.0.a1
pyrus_communis pycom03g17110
rosa_chinensis RchiOBHm_Chr5g0034861
rosa_laevigata RLG00000033581
rosa_multiflora Rmu_sc0002460.1_g000038
rosa_roxburghii Rroxscaffold_1G00045470
rosa_rugosa Rorug05G0148700
rosa_samantha Rh5AG241100 Rh5BG241300 Rh5CG272300 Rh5DG249800
rosa_wichuraiana Rw5G022040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 2 cut(s) 29, 130
AfiI CCNNNNNNNGG 1 cut(s) 191
AflII CTTAAG 1 cut(s) 268
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 1 cut(s) 154
AluBI AGCT 1 cut(s) 267
AluI AGCT 1 cut(s) 267
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 241
AoxI GGCC 1 cut(s) 110
ApoI RAATTY 1 cut(s) 286
AspLEI GCGC 1 cut(s) 85
AsuC2I CCSGG 2 cut(s) 147, 189
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 137
BcnI CCSGG 2 cut(s) 147, 189
BcoDI GTCTC 1 cut(s) 241
BfmI CTRYAG 1 cut(s) 22
BfoI RGCGCY 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 268
BglI GCCNNNNNGGC 1 cut(s) 307
Bme1390I CCNGG 2 cut(s) 147, 189
BmrFI CCNGG 2 cut(s) 147, 189
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 288
BmuI ACTGGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 147, 189
BsaAI YACGTR 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 107, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse118I RCCGGY 1 cut(s) 165
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 107, 113
BseGI GGATG 1 cut(s) 101
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMI GCAATG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 2 cut(s) 220, 250
BshFI GGCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 3 cut(s) 147, 166, 188
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 1 cut(s) 191
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 137
BspANI GGCC 1 cut(s) 112
BspTI CTTAAG 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 165
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 107, 113
BssT1I CCWWGG 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 268
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 167
BstF5I GGATG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 145, 187
BstSFI CTRYAG 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 266
BsuRI GGCC 1 cut(s) 112
BtsCI GGATG 1 cut(s) 101
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 167
CfoI GCGC 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 165
Csp6I GTAC 2 cut(s) 28, 129
CviAII CATG 1 cut(s) 319
CviJI RGCY 6 cut(s) 43, 112, 165, 267, 310, 329
CviKI_1 RGCY 6 cut(s) 43, 112, 165, 267, 310, 329
CviQI GTAC 2 cut(s) 28, 129
Eco130I CCWWGG 1 cut(s) 107
Eco147I AGGCCT 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 1 cut(s) 322
FaiI YATR 6 cut(s) 77, 127, 177, 216, 252, 320
FaqI GGGAC 1 cut(s) 129
FatI CATG 1 cut(s) 318
FokI GGATG 1 cut(s) 88
GlaI GCGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 1 cut(s) 112
HapII CCGG 3 cut(s) 147, 166, 188
HhaI GCGC 1 cut(s) 85
Hin1II CATG 1 cut(s) 322
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HinfI GANTC 1 cut(s) 139
HpaII CCGG 3 cut(s) 147, 166, 188
Hpy188I TCNGA 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 3 cut(s) 4, 179, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 307
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 1 cut(s) 322
HspAI GCGC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 165
KroNI GCCGGC 1 cut(s) 167
LpnPI CCDG 7 cut(s) 10, 107, 142, 160, 179, 201, 316
LweI GCATC 1 cut(s) 288
MaeII ACGT 1 cut(s) 131
MboII GAAGA 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 1 cut(s) 286
MmeI TCCRAC 1 cut(s) 316
MroNI GCCGGC 1 cut(s) 165
MseI TTAA 2 cut(s) 269, 331
MslI CAYNNNNRTG 2 cut(s) 174, 323
MspCI CTTAAG 1 cut(s) 268
MspI CCGG 3 cut(s) 147, 166, 188
MspR9I CCNGG 2 cut(s) 147, 189
MwoI GCNNNNNNNGC 1 cut(s) 307
NaeI GCCGGC 1 cut(s) 167
NciI CCSGG 2 cut(s) 147, 189
NgoMIV GCCGGC 1 cut(s) 165
NlaIII CATG 1 cut(s) 322
NspI RCATGY 1 cut(s) 322
PceI AGGCCT 1 cut(s) 112
PciI ACATGT 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 139
PfoI TCCNGGA 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 132
PscI ACATGT 1 cut(s) 318
RsaI GTAC 2 cut(s) 29, 130
RsaNI GTAC 2 cut(s) 28, 129
RseI CAYNNNNRTG 2 cut(s) 174, 323
SaqAI TTAA 2 cut(s) 269, 331
ScrFI CCNGG 2 cut(s) 147, 189
SduI GDGCHC 1 cut(s) 137
SetI ASST 6 cut(s) 29, 126, 134, 161, 231, 269
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 174, 323
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 1 cut(s) 286
SseBI AGGCCT 1 cut(s) 112
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 2 cut(s) 145, 187
StyI CCWWGG 1 cut(s) 107
TaiI ACGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 137
TasI AATT 1 cut(s) 286
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 2 cut(s) 269, 331
Tru9I TTAA 2 cut(s) 269, 331
TscAI CASTG 1 cut(s) 61
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 1 cut(s) 61
Vha464I CTTAAG 1 cut(s) 268
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.