Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010960

Physical Location & Seq

Genomic Coordinates Forward (+)

34370869 .. 34373943

Gene Structure 3075 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1256200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGGAAGCATCACATTTTCTACAAAGCAGGAGCTTTGTAACGACAACTATGGCTCGGCCAAGGCATGTGCATGAACGCAACAAGCTACGGCGTTGTTCGATTGTTGCGGTGCTTACAACGCCGGCGGAGAACATGAACATAGCAACAGATACAAGACCGGCAGGTGCTGCAGTAGAAGACAAGAGAGTCTACAAAGATAACTGGTTCGATCGAATGGCCATTAATCATCTTTCACAGAATGTCCAAGCTGCAACAAGGTTGAGAAACAATAAGAGCGGGTACGAAAGCTTGGTGGAGGCAGCAACAGTTGCATCCAGGAAGTTTAATCCAGAAAAACAGAGAGAACTAGTTCTTCAAGCTCTTGATACTGCCTTCCCAAAGCCCGTATTTTCCTTGCTTAGGACAATCCTACCAGAATCAAAATTTGCCAGGGAATACTTCGCAATCTTCACCACCATTTTCTTTGCCTGGTTGGTGGGACCCTGTGAGGTAAAAGAATCGGAGATCAATGGAAGAAAAGAGAAAAATGTAGTGTACATCAAGAAATGCAGGTTTTTAGAGGAAAGCAATTGTGTAGGGATGTGCCTTAATATGTGCAAGGTGCCATCTCAAAGCTTCATCAAGACTTCCCTTGGAACTCCAGTCAACATGGTTCCAAATTTTGATGATATGAGCTGTGAGATGATATTTGGTCAGAATCCTCCAGAGATATCCAACGATCCAGCACTCAAGCAACCATGCTACAAACTATGTAATACTTCATCATTTTACTCGGTTTTATTAGCTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009653 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010817 GO:0016106 GO:0016114 GO:0016853 GO:0016859 GO:0018130 GO:0032501 GO:0032502 GO:0042445 GO:0042446 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046872 GO:0046914 GO:0048367 GO:0048646 GO:0048731 GO:0048856 GO:0065007 GO:0065008 GO:0071704 GO:1901334 GO:1901336 GO:1901360 GO:1901362 GO:1901576 GO:1901600 GO:1901601 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko00906 map00906

Pfam Domains

Protein Families

DUF4033

Protein Analysis

264

Amino Acids

29.84

Weight (kDa)

9.04

Isoelectric Point (pI)

50.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
D27-like_C	PF13225	157 - 238	4.3e-35	Beta-carotene isomerase D27-like, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0010960)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03055 AT1G03055
fragaria_vesca	FvH4_2g22790
malus_domestica	MD03G1256200.v1.1 MD11G1277000.v1.1
prunus_persica	Prupe.8G233300_v2.0.a1
pyrus_communis	pycom11g24500
rosa_chinensis	RchiOBHm_Chr6g0289711
rosa_laevigata	RLG00000012273
rosa_multiflora	Rmu_sc0005640.1_g000030
rosa_roxburghii	Rroxscaffold_7G00177620 Rroxscaffold_7G00178120
rosa_rugosa	Rorug06G0205900 Rorug06G0206000 Rorug06G0206000
rosa_samantha	Rh6AG317600 Rh6BG325200 Rh6CG330800 Rh6DG316500
rosa_wichuraiana	Rw6G027390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	152
AasI	GACNNNNNNGTC	1 cut(s)	185
Acc36I	ACCTGC	2 cut(s)	152, 540
AccB1I	GGYRCC	1 cut(s)	601
AccBSI	CCGCTC	1 cut(s)	276
AccI	GTMKAC	1 cut(s)	189
AciI	CCGC	3 cut(s)	107, 125, 276
AclWI	GGATC	1 cut(s)	713
AcoI	YGGCCR	2 cut(s)	56, 216
AcsI	RAATTY	2 cut(s)	422, 658
AfaI	GTAC	2 cut(s)	281, 536
AfiI	CCNNNNNNNGG	1 cut(s)	399
AgsI	TTSAA	1 cut(s)	356
AhlI	ACTAGT	1 cut(s)	346
AjnI	CCWGG	3 cut(s)	314, 428, 467
AluBI	AGCT	8 cut(s)	33, 85, 248, 288, 359, 615, 675, 785
AluI	AGCT	8 cut(s)	33, 85, 248, 288, 359, 615, 675, 785
AlwI	GGATC	1 cut(s)	713
AlwNI	CAGNNNCTG	1 cut(s)	167
AoxI	GGCC	2 cut(s)	56, 216
ApeKI	GCWGC	3 cut(s)	167, 248, 299
ApoI	RAATTY	2 cut(s)	422, 658
AseI	ATTAAT	1 cut(s)	222
Asp700I	GAANNNNTTC	1 cut(s)	348
AspS9I	GGNCC	1 cut(s)	479
AsuHPI	GGTGA	1 cut(s)	442
AvaII	GGWCC	1 cut(s)	479
BalI	TGGCCA	1 cut(s)	218
BanI	GGYRCC	1 cut(s)	601
BbsI	GAAGAC	1 cut(s)	183
BbvI	GCAGC	3 cut(s)	154, 235, 311
BccI	CCATC	1 cut(s)	613
BceAI	ACGGC	1 cut(s)	104
BciT130I	CCWGG	3 cut(s)	316, 430, 469
BcuI	ACTAGT	1 cut(s)	346
BfaI	CTAG	1 cut(s)	347
BfmI	CTRYAG	1 cut(s)	168
BfuAI	ACCTGC	2 cut(s)	152, 540
BisI	GCNGC	3 cut(s)	168, 249, 300
BlsI	GCNGC	3 cut(s)	169, 250, 301
Bme1390I	CCNGG	3 cut(s)	316, 430, 469
Bme18I	GGWCC	1 cut(s)	479
BmgT120I	GGNCC	1 cut(s)	479
BmiI	GGNNCC	4 cut(s)	480, 481, 603, 654
BmrFI	CCNGG	3 cut(s)	316, 430, 469
BmsI	GCATC	2 cut(s)	17, 320
BpiI	GAAGAC	1 cut(s)	183
BpmI	CTGGAG	2 cut(s)	624, 687
Bpu10I	CCTNAGC	1 cut(s)	398
BpuEI	CTTGAG	1 cut(s)	713
BsaJI	CCNNGG	3 cut(s)	59, 429, 631
Bsc4I	CCNNNNNNNGG	1 cut(s)	399
Bse118I	RCCGGY	2 cut(s)	121, 157
Bse1I	ACTGG	2 cut(s)	206, 641
BseBI	CCWGG	3 cut(s)	316, 430, 469
BseDI	CCNNGG	3 cut(s)	59, 429, 631
BseGI	GGATG	2 cut(s)	311, 585
BseLI	CCNNNNNNNGG	1 cut(s)	399
BseNI	ACTGG	2 cut(s)	206, 641
BseXI	GCAGC	3 cut(s)	154, 235, 311
Bsh1285I	CGRYCG	1 cut(s)	211
BshFI	GGCC	2 cut(s)	58, 218
BshNI	GGYRCC	1 cut(s)	601
BsiEI	CGRYCG	1 cut(s)	211
BsiSI	CCGG	2 cut(s)	122, 158
BslFI	GGGAC	1 cut(s)	492
BslI	CCNNNNNNNGG	1 cut(s)	399
BsmFI	GGGAC	1 cut(s)	492
BsnI	GGCC	2 cut(s)	58, 218
Bsp1407I	TGTACA	1 cut(s)	534
Bsp143I	GATC	3 cut(s)	208, 504, 718
BspACI	CCGC	3 cut(s)	107, 125, 276
BspANI	GGCC	2 cut(s)	58, 218
BspLI	GGNNCC	4 cut(s)	480, 481, 603, 654
BspMAI	CTGCAG	1 cut(s)	172
BspMI	ACCTGC	2 cut(s)	152, 540
BspPI	GGATC	1 cut(s)	713
BspT107I	GGYRCC	1 cut(s)	601
BsrBI	CCGCTC	1 cut(s)	276
BsrFI	RCCGGY	2 cut(s)	121, 157
BsrGI	TGTACA	1 cut(s)	534
BsrI	ACTGG	2 cut(s)	206, 641
BssAI	RCCGGY	2 cut(s)	121, 157
BssECI	CCNNGG	3 cut(s)	59, 429, 631
BssMI	GATC	3 cut(s)	208, 504, 718
BssT1I	CCWWGG	2 cut(s)	59, 631
Bst2UI	CCWGG	3 cut(s)	316, 430, 469
Bst4CI	ACNGT	1 cut(s)	307
BstAPI	GCANNNNNTGC	2 cut(s)	167, 308
BstAUI	TGTACA	1 cut(s)	534
BstC8I	GCNNGC	1 cut(s)	123
BstDEI	CTNAG	1 cut(s)	398
BstENI	CCTNNNNNAGG	1 cut(s)	397
BstF5I	GGATG	2 cut(s)	311, 585
BstKTI	GATC	3 cut(s)	211, 507, 721
BstMBI	GATC	3 cut(s)	208, 504, 718
BstMCI	CGRYCG	1 cut(s)	211
BstMWI	GCNNNNNNNGC	3 cut(s)	118, 167, 308
BstNI	CCWGG	3 cut(s)	316, 430, 469
BstNSI	RCATGY	1 cut(s)	68
BstSCI	CCNGG	3 cut(s)	314, 428, 467
BstSFI	CTRYAG	1 cut(s)	168
BstV1I	GCAGC	3 cut(s)	154, 235, 311
BstV2I	GAAGAC	1 cut(s)	183
BsuRI	GGCC	2 cut(s)	58, 218
BtsCI	GGATG	2 cut(s)	311, 585
BveI	ACCTGC	2 cut(s)	152, 540
Cac8I	GCNNGC	1 cut(s)	123
CaiI	CAGNNNCTG	1 cut(s)	167
Cfr10I	RCCGGY	2 cut(s)	121, 157
Cfr13I	GGNCC	1 cut(s)	479
Csp6I	GTAC	2 cut(s)	280, 535
CviAII	CATG	5 cut(s)	65, 71, 133, 649, 738
CviQI	GTAC	2 cut(s)	280, 535
DdeI	CTNAG	1 cut(s)	398
DpnI	GATC	3 cut(s)	210, 506, 720
DpnII	GATC	3 cut(s)	208, 504, 718
DrdI	GACNNNNNNGTC	1 cut(s)	185
DseDI	GACNNNNNNGTC	1 cut(s)	185
EaeI	YGGCCR	2 cut(s)	56, 216
EciI	GGCGGA	1 cut(s)	140
Eco130I	CCWWGG	2 cut(s)	59, 631
Eco32I	GATATC	1 cut(s)	711
Eco47I	GGWCC	1 cut(s)	479
EcoNI	CCTNNNNNAGG	1 cut(s)	397
EcoO109I	RGGNCCY	1 cut(s)	479
EcoRII	CCWGG	3 cut(s)	314, 428, 467
EcoRV	GATATC	1 cut(s)	711
EcoT14I	CCWWGG	2 cut(s)	59, 631
ErhI	CCWWGG	2 cut(s)	59, 631
FaeI	CATG	5 cut(s)	68, 74, 136, 652, 741
FaqI	GGGAC	1 cut(s)	492
FatI	CATG	5 cut(s)	64, 70, 132, 648, 737
FauI	CCCGC	1 cut(s)	269
FblI	GTMKAC	1 cut(s)	189
Fnu4HI	GCNGC	3 cut(s)	168, 249, 300
FokI	GGATG	2 cut(s)	298, 592
Fsp4HI	GCNGC	3 cut(s)	168, 249, 300
FspBI	CTAG	1 cut(s)	347
GluI	GCNGC	3 cut(s)	168, 249, 300
GsuI	CTGGAG	2 cut(s)	624, 687
HaeIII	GGCC	2 cut(s)	58, 218
HapII	CCGG	2 cut(s)	122, 158
Hin1II	CATG	5 cut(s)	68, 74, 136, 652, 741
HincII	GTYRAC	1 cut(s)	646
HindII	GTYRAC	1 cut(s)	646
HindIII	AAGCTT	2 cut(s)	286, 613
HinfI	GANTC	4 cut(s)	186, 416, 497, 697
HpaII	CCGG	2 cut(s)	122, 158
HphI	GGTGA	1 cut(s)	442
Hpy166II	GTNNAC	3 cut(s)	190, 535, 646
Hpy188I	TCNGA	2 cut(s)	502, 696
Hpy188III	TCNNGA	5 cut(s)	329, 362, 541, 622, 704
Hpy8I	GTNNAC	3 cut(s)	190, 535, 646
HpyAV	CCTTC	1 cut(s)	382
HpyCH4III	ACNGT	1 cut(s)	307
HpyCH4V	TGCA	6 cut(s)	70, 170, 251, 311, 549, 597
HpyF10VI	GCNNNNNNNGC	3 cut(s)	118, 167, 308
HpyF3I	CTNAG	1 cut(s)	398
Hsp92II	CATG	5 cut(s)	68, 74, 136, 652, 741
KflI	GGGWCCC	1 cut(s)	479
KroI	GCCGGC	1 cut(s)	121
KroNI	GCCGGC	1 cut(s)	123
Kzo9I	GATC	3 cut(s)	208, 504, 718
LmnI	GCTCC	1 cut(s)	30
Lsp1109I	GCAGC	3 cut(s)	154, 235, 311
LweI	GCATC	2 cut(s)	17, 320
MaeI	CTAG	1 cut(s)	347
MaeIII	GTNAC	1 cut(s)	37
MalI	GATC	3 cut(s)	210, 506, 720
MbiI	CCGCTC	1 cut(s)	276
MboI	GATC	3 cut(s)	208, 504, 718
MboII	GAAGA	4 cut(s)	188, 344, 439, 525
MfeI	CAATTG	1 cut(s)	568
MlsI	TGGCCA	1 cut(s)	218
MluCI	AATT	3 cut(s)	422, 568, 658
MluNI	TGGCCA	1 cut(s)	218
MlyI	GAGTC	1 cut(s)	195
MmeI	TCCRAC	1 cut(s)	738
MnlI	CCTC	4 cut(s)	289, 481, 553, 711
Mox20I	TGGCCA	1 cut(s)	218
MreI	CGCCGGCG	1 cut(s)	121
MroNI	GCCGGC	1 cut(s)	121
MroXI	GAANNNNTTC	1 cut(s)	348
MscI	TGGCCA	1 cut(s)	218
MseI	TTAA	3 cut(s)	222, 324, 588
MslI	CAYNNNNRTG	1 cut(s)	69
Msp20I	TGGCCA	1 cut(s)	218
MspI	CCGG	2 cut(s)	122, 158
MspR9I	CCNGG	3 cut(s)	316, 430, 469
MunI	CAATTG	1 cut(s)	568
MvaI	CCWGG	3 cut(s)	316, 430, 469
MwoI	GCNNNNNNNGC	3 cut(s)	118, 167, 308
NaeI	GCCGGC	1 cut(s)	123
NdeII	GATC	3 cut(s)	208, 504, 718
NgoMIV	GCCGGC	1 cut(s)	121
NlaIII	CATG	5 cut(s)	68, 74, 136, 652, 741
NlaIV	GGNNCC	4 cut(s)	480, 481, 603, 654
NmeAIII	GCCGAG	1 cut(s)	34
NspI	RCATGY	1 cut(s)	68
PaqCI	CACCTGC	1 cut(s)	152
PdiI	GCCGGC	1 cut(s)	123
PdmI	GAANNNNTTC	1 cut(s)	348
PfeI	GAWTC	3 cut(s)	416, 497, 697
PfoI	TCCNGGA	1 cut(s)	314
PkrI	GCNGC	3 cut(s)	169, 250, 301
Ple19I	CGATCG	1 cut(s)	211
PleI	GAGTC	1 cut(s)	194
PpsI	GAGTC	1 cut(s)	194
PpuMI	RGGWCCY	1 cut(s)	479
PshBI	ATTAAT	1 cut(s)	222
Psp5II	RGGWCCY	1 cut(s)	479
Psp6I	CCWGG	3 cut(s)	314, 428, 467
PspGI	CCWGG	3 cut(s)	314, 428, 467
PspN4I	GGNNCC	4 cut(s)	480, 481, 603, 654
PspPI	GGNCC	1 cut(s)	479
PspPPI	RGGWCCY	1 cut(s)	479
PstI	CTGCAG	1 cut(s)	172
PstNI	CAGNNNCTG	1 cut(s)	167
PvuI	CGATCG	1 cut(s)	211
RsaI	GTAC	2 cut(s)	281, 536
RsaNI	GTAC	2 cut(s)	280, 535
RseI	CAYNNNNRTG	1 cut(s)	69
SaqAI	TTAA	3 cut(s)	222, 324, 588
SatI	GCNGC	3 cut(s)	168, 249, 300
Sau3AI	GATC	3 cut(s)	208, 504, 718
Sau96I	GGNCC	1 cut(s)	479
SchI	GAGTC	1 cut(s)	195
ScrFI	CCNGG	3 cut(s)	316, 430, 469
SfaNI	GCATC	2 cut(s)	17, 320
SfcI	CTRYAG	1 cut(s)	168
SgrAI	CRCCGGYG	1 cut(s)	121
SinI	GGWCC	1 cut(s)	479
SmiMI	CAYNNNNRTG	1 cut(s)	69
SmlI	CTYRAG	1 cut(s)	728
SmoI	CTYRAG	1 cut(s)	728
SpeI	ACTAGT	1 cut(s)	346
Sse9I	AATT	3 cut(s)	422, 568, 658
SsiI	CCGC	3 cut(s)	107, 125, 276
SspMI	CTAG	1 cut(s)	347
StyD4I	CCNGG	3 cut(s)	314, 428, 467
StyI	CCWWGG	2 cut(s)	59, 631
TaaI	ACNGT	1 cut(s)	307
TaqI	TCGA	3 cut(s)	98, 207, 211
TasI	AATT	3 cut(s)	422, 568, 658
TatI	WGTACW	1 cut(s)	534
TfiI	GAWTC	3 cut(s)	416, 497, 697
Tru1I	TTAA	3 cut(s)	222, 324, 588
Tru9I	TTAA	3 cut(s)	222, 324, 588
TseI	GCWGC	3 cut(s)	167, 248, 299
TspDTI	ATGAA	4 cut(s)	87, 149, 607, 750
VpaK11BI	GGWCC	1 cut(s)	479
VspI	ATTAAT	1 cut(s)	222
XagI	CCTNNNNNAGG	1 cut(s)	397
XapI	RAATTY	2 cut(s)	422, 658
XceI	RCATGY	1 cut(s)	68
XmiI	GTMKAC	1 cut(s)	189
XmnI	GAANNNNTTC	1 cut(s)	348
XspI	CTAG	1 cut(s)	347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.