MD03G1258200.v1.1

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009751
Physical Location & Seq
Reverse (-)
34526653 .. 34530186
3534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1258200.v1.1.491

Sequence Viewer

Length: 1395 bp
ATGTTTCCTATCTCTATGTCCAATTCAACTTCTTTGTCTGAGGAAGGTAATAGTGTTTCTTCAGACTTTGCTGCTGCCTTAAACCCTCTTCTCCTTTCCACCAATATTTCTCCTCATCAGCATGATCAGCCACCTAACAAAATAAAAAAGAAAAGAAGCCTCCCCGGAAACCCAGACCCAGATGCTGAAGTGATTGCATTATCCCCCAAAACCCTACTTGCCACAAACAGATTTGTGTGTGAGATTTGCAACAAGGGTTTTCAGAGAGATCAGAACCTTCAGCTTCACAGGAGAGGCCACAACCTTCCATGGAAGCTGAAGCAAAGGAACAGCAAGGATCAGATCAGAAAGAGAGCCTATGTATGCCCTGAGCCCTCATGTGTTCATCACCACCCTTCAAGAGCACTTGGTGACCTCACAGGGATCAAAAAGCACTTTTGCAGGAAGCACGGGGAGAAGAAGTGGAAGTGTGACAAGTGCTCCAAGATCTATGCTGTTCAATCTGATTGGAAGGCTCACTCCAAAACCTGTGGTACAAGAGAATATAGATGTGACTGTGGAACCCTTTTCTCCAGGAAGGATAGCTTCATAACTCACAGGGCGTTCTGTGATGCTTTGGCTGAAGAAAGTGCGAGACTCTCAGCAAACCAATTTGCTACAGCCTCCATCACCGCCGCCACAATTAAACCTCAAATCTCCCTCTTCCCTTTTCCAACTCACCAACAACAACACTTCACAAACCCAAACCCACCATTGCCACCCTTCACTACAATCTCCCTCACCCAATGGGAACCCCCACAACCTCATCAAAACCCTAACCCTAGCCCGAATCCCACCCAAAACTGCCCACTCCATCACCTCATCAAGCCCGAATCTCATCCCCATTTCCCACCACCACCACAACCCCACAAGGGTTTGATCATCTCACCCTTCCAAAACCTCCATGTGAGCTCACAACCCAACTCCAACCCCACTACGTCATCTGCCCACCTTTCAGCTACTGCACTCCTCCAAAAGGCGGCAACCGTTGGTGCAGCAGTAGCCTCTGGAGCCCAACAGGGACAGTCAGCGGGCTTGACCGTGGGAGAGTTTGGGACCGCGAGCCACATGATCTCCACAGAGTATCTAGGCGGGTTCGCGTCCGGAGAGCTCGCGACGTGGCGCAAGACTGACAGTCTGACAAGAGACTTTCTAGGTCTGAACGGGGATGGCACGGGGCATAATAATAATAACGTTCGCGGTGTTCACGTCAAGGACATGCTAACGTACGCCGGTGGCGTAGGGTTCCATCAGCCGCCGTACGAGCGTCTTCGTGGCCATGATTCGCTGCTGAAGCCCCAGGGTTTCGGGTTCGCTGAGACTACTACTGCTTCGGAAACGTGGGGAGACTGTTGA

Protein Analysis

465

Amino Acids

50.95

Weight (kDa)

9.16

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 116 - 150 3.4e-21 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 154 - 178 2.5e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 181 - 219 1.5e-23 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 1100, 1139, 1154, 1239
AccIII TCCGGA 1 cut(s) 1142
AciI CCGC 8 cut(s) 672, 675, 1019, 1070, 1098, 1131, 1239, 1295
AclI AACGTT 1 cut(s) 1233
AclWI GGATC 2 cut(s) 345, 431
AcoI YGGCCR 1 cut(s) 1315
AcuI CTGAAG 6 cut(s) 45, 207, 263, 338, 642, 1352
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 3 cut(s) 535, 1268, 1301
AfiI CCNNNNNNNGG 3 cut(s) 911, 1015, 1018
AgsI TTSAA 3 cut(s) 27, 399, 500
AhdI GACNNNNNGTC 1 cut(s) 1173
AjiI CACGTC 2 cut(s) 1158, 1249
AjnI CCWGG 2 cut(s) 572, 1338
AluBI AGCT 6 cut(s) 283, 316, 585, 951, 998, 1150
AluI AGCT 6 cut(s) 283, 316, 585, 951, 998, 1150
Alw21I GWGCWC 4 cut(s) 406, 482, 953, 1152
Alw26I GTCTC 4 cut(s) 628, 1179, 1352, 1380
AlwI GGATC 2 cut(s) 345, 431
AlwNI CAGNNNCTG 2 cut(s) 185, 1001
Aor13HI TCCGGA 1 cut(s) 1142
AoxI GGCC 2 cut(s) 295, 1315
ApeKI GCWGC 4 cut(s) 71, 74, 1034, 1327
AspLEI GCGC 1 cut(s) 1164
AspS9I GGNCC 1 cut(s) 1095
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 7 cut(s) 380, 422, 661, 710, 772, 848, 918
AvaII GGWCC 1 cut(s) 1095
BalI TGGCCA 1 cut(s) 1317
BanII GRGCYC 4 cut(s) 375, 953, 1054, 1152
BbsI GAAGAC 1 cut(s) 1301
Bbv12I GWGCWC 4 cut(s) 406, 482, 953, 1152
BbvI GCAGC 4 cut(s) 58, 61, 1046, 1314
BccI CCATC 4 cut(s) 674, 861, 1202, 1296
BceAI ACGGC 1 cut(s) 1282
BciT130I CCWGG 2 cut(s) 574, 1340
BclI TGATCA 2 cut(s) 124, 918
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 4 cut(s) 628, 1179, 1352, 1380
BfaI CTAG 3 cut(s) 822, 1127, 1193
BfmI CTRYAG 1 cut(s) 657
BglII AGATCT 1 cut(s) 486
BisI GCNGC 7 cut(s) 72, 75, 675, 1020, 1035, 1295, 1328
BlsI GCNGC 7 cut(s) 73, 76, 676, 1021, 1036, 1296, 1329
Bme1390I CCNGG 3 cut(s) 165, 574, 1340
Bme18I GGWCC 1 cut(s) 1095
BmeRI GACNNNNNGTC 1 cut(s) 1173
BmgBI CACGTC 2 cut(s) 1158, 1249
BmgT120I GGNCC 1 cut(s) 1095
BmiI GGNNCC 5 cut(s) 562, 792, 1051, 1096, 1286
BmrFI CCNGG 3 cut(s) 165, 574, 1340
BmsI GCATC 2 cut(s) 172, 601
BpiI GAAGAC 1 cut(s) 1301
BpmI CTGGAG 2 cut(s) 556, 1068
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 165
BsaJI CCNNGG 5 cut(s) 163, 308, 1080, 1338, 1339
BsaWI WCCGGW 1 cut(s) 1142
BsaXI ACNNNNNCTCC 6 cut(s) 446, 464, 476, 494, 1097, 1127
Bsc4I CCNNNNNNNGG 3 cut(s) 911, 1015, 1018
Bse118I RCCGGY 1 cut(s) 1271
Bse3DI GCAATG 1 cut(s) 752
BseAI TCCGGA 1 cut(s) 1142
BseBI CCWGG 2 cut(s) 574, 1340
BseDI CCNNGG 5 cut(s) 163, 308, 1080, 1338, 1339
BseGI GGATG 2 cut(s) 877, 1213
BseLI CCNNNNNNNGG 3 cut(s) 911, 1015, 1018
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 4 cut(s) 30, 360, 654, 1347
BseRI GAGGAG 2 cut(s) 102, 998
BseXI GCAGC 4 cut(s) 58, 61, 1046, 1314
BsgI GTGCAG 2 cut(s) 987, 1053
Bsh1236I CGCG 4 cut(s) 1100, 1139, 1154, 1239
BshFI GGCC 2 cut(s) 297, 1317
BsiHKAI GWGCWC 4 cut(s) 406, 482, 953, 1152
BsiSI CCGG 3 cut(s) 165, 1143, 1272
BsiWI CGTACG 2 cut(s) 1266, 1299
BslFI GGGAC 2 cut(s) 1074, 1108
BslI CCNNNNNNNGG 3 cut(s) 911, 1015, 1018
BsmAI GTCTC 4 cut(s) 628, 1179, 1352, 1380
BsmFI GGGAC 2 cut(s) 1074, 1108
BsnI GGCC 2 cut(s) 297, 1317
Bsp1286I GDGCHC 6 cut(s) 375, 406, 482, 953, 1054, 1152
Bsp13I TCCGGA 1 cut(s) 1142
Bsp143I GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
Bsp19I CCATGG 1 cut(s) 308
Bsp68I TCGCGA 1 cut(s) 1154
BspACI CCGC 8 cut(s) 672, 675, 1019, 1070, 1098, 1131, 1239, 1295
BspANI GGCC 2 cut(s) 297, 1317
BspCNI CTCAG 4 cut(s) 31, 361, 653, 1348
BspEI TCCGGA 1 cut(s) 1142
BspFNI CGCG 4 cut(s) 1100, 1139, 1154, 1239
BspLI GGNNCC 5 cut(s) 562, 792, 1051, 1096, 1286
BspPI GGATC 2 cut(s) 345, 431
BsrDI GCAATG 1 cut(s) 752
BsrFI RCCGGY 1 cut(s) 1271
BssAI RCCGGY 1 cut(s) 1271
BssECI CCNNGG 5 cut(s) 163, 308, 1080, 1338, 1339
BssMI GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 2 cut(s) 574, 1340
Bst4CI ACNGT 6 cut(s) 557, 1027, 1065, 1081, 1175, 1391
Bst6I CTCTTC 2 cut(s) 93, 707
BstC8I GCNNGC 3 cut(s) 1072, 1102, 1152
BstDEI CTNAG 4 cut(s) 39, 369, 640, 1356
BstDSI CCRYGG 2 cut(s) 308, 1080
BstEII GGTNACC 1 cut(s) 410
BstENI CCTNNNNNAGG 1 cut(s) 1013
BstF5I GGATG 2 cut(s) 877, 1213
BstFNI CGCG 4 cut(s) 1100, 1139, 1154, 1239
BstHHI GCGC 1 cut(s) 1164
BstKTI GATC 8 cut(s) 127, 271, 340, 345, 426, 489, 921, 1113
BstMAI GTCTC 4 cut(s) 628, 1179, 1352, 1380
BstMBI GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
BstMWI GCNNNNNNNGC 5 cut(s) 127, 1040, 1049, 1303, 1333
BstNI CCWGG 2 cut(s) 574, 1340
BstNSI RCATGY 1 cut(s) 1261
BstPI GGTNACC 1 cut(s) 410
BstSCI CCNGG 3 cut(s) 163, 572, 1338
BstSFI CTRYAG 1 cut(s) 657
BstUI CGCG 4 cut(s) 1100, 1139, 1154, 1239
BstV1I GCAGC 4 cut(s) 58, 61, 1046, 1314
BstV2I GAAGAC 1 cut(s) 1301
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
BsuRI GGCC 2 cut(s) 297, 1317
BtgI CCRYGG 2 cut(s) 308, 1080
BtrI CACGTC 2 cut(s) 1158, 1249
BtsCI GGATG 2 cut(s) 877, 1213
BtuMI TCGCGA 1 cut(s) 1154
Cac8I GCNNGC 3 cut(s) 1072, 1102, 1152
CaiI CAGNNNCTG 2 cut(s) 185, 1001
CfoI GCGC 1 cut(s) 1164
Cfr10I RCCGGY 1 cut(s) 1271
Cfr13I GGNCC 1 cut(s) 1095
CseI GACGC 2 cut(s) 1128, 1295
Csp6I GTAC 3 cut(s) 534, 1267, 1300
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviAII CATG 7 cut(s) 122, 309, 378, 944, 1108, 1258, 1319
CviQI GTAC 3 cut(s) 534, 1267, 1300
DdeI CTNAG 4 cut(s) 39, 369, 640, 1356
DpnI GATC 8 cut(s) 126, 270, 339, 344, 425, 488, 920, 1112
DpnII GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
DraIII CACNNNGTG 1 cut(s) 410
DriI GACNNNNNGTC 1 cut(s) 1173
EaeI YGGCCR 1 cut(s) 1315
Eam1104I CTCTTC 2 cut(s) 93, 707
Eam1105I GACNNNNNGTC 1 cut(s) 1173
EarI CTCTTC 2 cut(s) 93, 707
Ecl136II GAGCTC 2 cut(s) 951, 1150
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 4 cut(s) 375, 953, 1054, 1152
Eco47I GGWCC 1 cut(s) 1095
Eco53kI GAGCTC 2 cut(s) 951, 1150
Eco57I CTGAAG 6 cut(s) 45, 207, 263, 338, 642, 1352
Eco91I GGTNACC 1 cut(s) 410
EcoICRI GAGCTC 2 cut(s) 951, 1150
EcoNI CCTNNNNNAGG 1 cut(s) 1013
EcoO65I GGTNACC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 572, 1338
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 4 cut(s) 375, 953, 1054, 1152
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 7 cut(s) 125, 312, 381, 947, 1111, 1261, 1322
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 2 cut(s) 1074, 1108
FatI CATG 7 cut(s) 121, 308, 377, 943, 1107, 1257, 1318
FauI CCCGC 2 cut(s) 1063, 1124
FbaI TGATCA 2 cut(s) 124, 918
Fnu4HI GCNGC 7 cut(s) 72, 75, 675, 1020, 1035, 1295, 1328
FokI GGATG 2 cut(s) 864, 1220
FriOI GRGCYC 4 cut(s) 375, 953, 1054, 1152
Fsp4HI GCNGC 7 cut(s) 72, 75, 675, 1020, 1035, 1295, 1328
FspBI CTAG 3 cut(s) 822, 1127, 1193
GlaI GCGC 1 cut(s) 1163
GluI GCNGC 7 cut(s) 72, 75, 675, 1020, 1035, 1295, 1328
GsuI CTGGAG 2 cut(s) 556, 1068
HaeIII GGCC 2 cut(s) 297, 1317
HapII CCGG 3 cut(s) 165, 1143, 1272
HgaI GACGC 2 cut(s) 1128, 1295
HhaI GCGC 1 cut(s) 1164
Hin1II CATG 7 cut(s) 125, 312, 381, 947, 1111, 1261, 1322
Hin6I GCGC 1 cut(s) 1162
HinP1I GCGC 1 cut(s) 1162
HinfI GANTC 4 cut(s) 636, 829, 872, 1322
HpaII CCGG 3 cut(s) 165, 1143, 1272
HphI GGTGA 7 cut(s) 380, 422, 661, 710, 772, 848, 918
Hpy166II GTNNAC 1 cut(s) 1246
Hpy188III TCNNGA 4 cut(s) 399, 1047, 1143, 1153
Hpy8I GTNNAC 1 cut(s) 1246
Hpy99I CGWCG 1 cut(s) 1159
HpyAV CCTTC 8 cut(s) 38, 287, 314, 405, 505, 571, 772, 940
HpyCH4III ACNGT 6 cut(s) 557, 1027, 1065, 1081, 1175, 1391
HpyCH4IV ACGT 6 cut(s) 977, 1157, 1233, 1248, 1265, 1379
HpyCH4V TGCA 5 cut(s) 197, 249, 441, 1004, 1034
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 1040, 1049, 1303, 1333
HpyF3I CTNAG 4 cut(s) 39, 369, 640, 1356
HpySE526I ACGT 6 cut(s) 977, 1157, 1233, 1248, 1265, 1379
Hsp92II CATG 7 cut(s) 125, 312, 381, 947, 1111, 1261, 1322
HspAI GCGC 1 cut(s) 1162
Kpn2I TCCGGA 1 cut(s) 1142
Ksp22I TGATCA 2 cut(s) 124, 918
Kzo9I GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
LmnI GCTCC 2 cut(s) 485, 1049
Lsp1109I GCAGC 4 cut(s) 58, 61, 1046, 1314
LweI GCATC 2 cut(s) 172, 601
MaeI CTAG 3 cut(s) 822, 1127, 1193
MaeII ACGT 6 cut(s) 977, 1157, 1233, 1248, 1265, 1379
MaeIII GTNAC 3 cut(s) 410, 470, 551
MalI GATC 8 cut(s) 126, 270, 339, 344, 425, 488, 920, 1112
MboI GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
MboII GAAGA 6 cut(s) 51, 80, 469, 635, 694, 1301
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 6 cut(s) 375, 406, 482, 953, 1054, 1152
MlsI TGGCCA 1 cut(s) 1317
MluCI AATT 3 cut(s) 22, 650, 681
MluNI TGGCCA 1 cut(s) 1317
MlyI GAGTC 1 cut(s) 630
MmeI TCCRAC 2 cut(s) 737, 990
Mox20I TGGCCA 1 cut(s) 1317
MroI TCCGGA 1 cut(s) 1142
MscI TGGCCA 1 cut(s) 1317
MseI TTAA 2 cut(s) 80, 684
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 1317
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 3 cut(s) 165, 1143, 1272
MspR9I CCNGG 3 cut(s) 165, 574, 1340
MvaI CCWGG 2 cut(s) 574, 1340
MvnI CGCG 4 cut(s) 1100, 1139, 1154, 1239
MwoI GCNNNNNNNGC 5 cut(s) 127, 1040, 1049, 1303, 1333
NciI CCSGG 1 cut(s) 165
NcoI CCATGG 1 cut(s) 308
NdeII GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
NlaIII CATG 7 cut(s) 125, 312, 381, 947, 1111, 1261, 1322
NlaIV GGNNCC 5 cut(s) 562, 792, 1051, 1096, 1286
NmuCI GTSAC 3 cut(s) 410, 470, 551
NruI TCGCGA 1 cut(s) 1154
NspI RCATGY 1 cut(s) 1261
PasI CCCWGGG 1 cut(s) 1339
PcsI WCGNNNNNNNCGW 1 cut(s) 1275
PfeI GAWTC 3 cut(s) 829, 872, 1322
Pfl23II CGTACG 2 cut(s) 1266, 1299
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 7 cut(s) 73, 76, 676, 1021, 1036, 1296, 1329
PleI GAGTC 1 cut(s) 630
PpsI GAGTC 1 cut(s) 630
Psp124BI GAGCTC 2 cut(s) 953, 1152
Psp1406I AACGTT 1 cut(s) 1233
Psp6I CCWGG 2 cut(s) 572, 1338
PspEI GGTNACC 1 cut(s) 410
PspGI CCWGG 2 cut(s) 572, 1338
PspLI CGTACG 2 cut(s) 1266, 1299
PspN4I GGNNCC 5 cut(s) 562, 792, 1051, 1096, 1286
PspPI GGNCC 1 cut(s) 1095
PstNI CAGNNNCTG 2 cut(s) 185, 1001
PsuI RGATCY 1 cut(s) 486
RruI TCGCGA 1 cut(s) 1154
RsaI GTAC 3 cut(s) 535, 1268, 1301
RsaNI GTAC 3 cut(s) 534, 1267, 1300
RseI CAYNNNNRTG 1 cut(s) 120
SacI GAGCTC 2 cut(s) 953, 1152
SaqAI TTAA 2 cut(s) 80, 684
SatI GCNGC 7 cut(s) 72, 75, 675, 1020, 1035, 1295, 1328
Sau3AI GATC 8 cut(s) 124, 268, 337, 342, 423, 486, 918, 1110
Sau96I GGNCC 1 cut(s) 1095
SchI GAGTC 1 cut(s) 630
ScrFI CCNGG 3 cut(s) 165, 574, 1340
SduI GDGCHC 6 cut(s) 375, 406, 482, 953, 1054, 1152
SfaNI GCATC 2 cut(s) 172, 601
SfcI CTRYAG 1 cut(s) 657
SgrAI CRCCGGYG 1 cut(s) 1271
SinI GGWCC 1 cut(s) 1095
SmiMI CAYNNNNRTG 1 cut(s) 120
Sse9I AATT 3 cut(s) 22, 650, 681
SsiI CCGC 8 cut(s) 672, 675, 1019, 1070, 1098, 1131, 1239, 1295
SspI AATATT 1 cut(s) 106
SspMI CTAG 3 cut(s) 822, 1127, 1193
SstI GAGCTC 2 cut(s) 953, 1152
StyD4I CCNGG 3 cut(s) 163, 572, 1338
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 6 cut(s) 557, 1027, 1065, 1081, 1175, 1391
TaiI ACGT 6 cut(s) 980, 1160, 1236, 1251, 1268, 1382
TasI AATT 3 cut(s) 22, 650, 681
TauI GCSGC 3 cut(s) 677, 1022, 1297
TfiI GAWTC 3 cut(s) 829, 872, 1322
Tru1I TTAA 2 cut(s) 80, 684
Tru9I TTAA 2 cut(s) 80, 684
TseFI GTSAC 3 cut(s) 410, 470, 551
TseI GCWGC 4 cut(s) 71, 74, 1034, 1327
Tsp45I GTSAC 3 cut(s) 410, 470, 551
TspDTI ATGAA 2 cut(s) 374, 577
VpaK11BI GGWCC 1 cut(s) 1095
XagI CCTNNNNNAGG 1 cut(s) 1013
XceI RCATGY 1 cut(s) 1261
XspI CTAG 3 cut(s) 822, 1127, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.