MD03G1259400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016094
Physical Location & Seq
Reverse (-)
34635436 .. 34637662
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1259400.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGAAGCCGCCGAAGTGCTACAACTCTATGAGCTCCGCTACTCCGACCTCGTACTCGTGTCGTCAAACGATGAATCATTGTCGTCGGCAGAAGAGCTCGAAAGGGCGGAGTCGACGAGCAAAGCCATAATGGAAGCCCTAGGTCCCACAGGCCCAGGTCTTCTCGCCATTACCGGCGTCCCCAAATCCGCCTCTCTCCGCCGTGCCCTCCTCCCTCTCGCTCGCAAGCTCGCCCTTCTCAACCCTAATCATCGCAAAGGCATTCTCAAGGATCACAGCTTGGGAAGTGATGTGCCTTTGAAAAACCCTGAGAGAAATGTGTCGTCTTTCGCAATGCAAATCAAATATTCACATGATTTTGATGAACCCCAATGGAATTCAGAACATGGATCCGTGAATGAGTTTGAGAATCTCGGAAATGGATTTAGGGAGTTGGGGTTTTGCATGATGGAGCTAGGCCTTAAACTTGCTCGAGTATGCGATCGTGCCATTGGTGGAAATGAGCTTGAGGAGAGCTTGTTGGAGTCCTGCTCTGCCAAAGCTCGTCTCATTCACTATCATTCACCGCTTGATAGTTCCGTTCTTGTTGAAGAAGCAATGAGCATGAAGAGAACATCGAAAAGACCCTTCAATTCTAGCAAAAATTGGATTGGTGACAAACGCAAACAAGTTTCGGGGATTTGCTCTGATAATCTGTGGCAGCAATGGCATTATGATTATGGGATTTTCACCGTCTTGACAGCTCCAATGTTTCTCTTGTCAAATCATCCACAAGCGAAAGAAGAAGGGGATCAAGAGTATGCTTACCCTAATGGAAATACTTATTTACAAATATTTGATCCCAGAAAGGATAATGTGTTCATGGTGAAGGCCTCTCCTGAGAGTTTTATAGTTCAGGTTGGCGAGTCGGCTAATATGATATCACGAGGAAAGCTCCGTGCCACCCTTCACTCTGTTTGCAGGCCTTCCAAGTTCGAAAATTTGAGCAGAGAAACTTTTGTTGTGTTCTTGCAGCCGGCGTGGAACAAAACTTTCTCCGTAACAGATTATTCCATGGACAAATCCTTGGAGATATCCTCAGAGGTCAAACAGGTTGATGACCAGGAGCAAAGAAGGCTAACTGAAGAAATACAGAAAATTGTACCACCTCTAGCATTGCGGTTGAAAGATGGGATGACATTTGCAGATTTCTCGCGCGAAACGACAAAGCAATACTATGGTGGATCTGGTTTGCAGTCAAATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy

Protein Analysis

416

Amino Acids

46.76

Weight (kDa)

5.94

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63290 AT3G63290
fragaria_vesca FvH4_2g23081
malus_domestica MD03G1259400.v1.1
prunus_persica Prupe.8G235900_v2.0.a1
pyrus_communis pycom03g20740
rosa_chinensis RchiOBHm_Chr6g0290231
rosa_laevigata RLG00000012232
rosa_roxburghii Rroxscaffold_7G00177220
rosa_rugosa Rorug06G0211100
rosa_samantha Rh6AG321400 Rh6BG328500 Rh6CG334500 Rh6DG321500
rosa_wichuraiana Rw6G027760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 113
AccII CGCG 2 cut(s) 1195, 1197
AciI CCGC 7 cut(s) 9, 37, 107, 189, 199, 566, 1159
AclWI GGATC 6 cut(s) 279, 384, 397, 798, 833, 1231
AcsI RAATTY 2 cut(s) 376, 979
AcuI CTGAAG 1 cut(s) 1143
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 2 cut(s) 54, 1143
AgsI TTSAA 4 cut(s) 301, 590, 631, 1165
AjnI CCWGG 2 cut(s) 154, 1101
AloI GAACNNNNNNTCC 2 cut(s) 842, 874
Alw21I GWGCWC 2 cut(s) 36, 99
Alw26I GTCTC 1 cut(s) 551
AlwI GGATC 6 cut(s) 279, 384, 397, 798, 833, 1231
Ama87I CYCGRG 1 cut(s) 471
AoxI GGCC 4 cut(s) 151, 457, 870, 962
ApeKI GCWGC 2 cut(s) 700, 1012
ApoI RAATTY 2 cut(s) 376, 979
AspA2I CCTAGG 1 cut(s) 139
AspLEI GCGC 1 cut(s) 1197
AspS9I GGNCC 2 cut(s) 143, 152
AsuHPI GGTGA 4 cut(s) 555, 665, 721, 877
AsuII TTCGAA 1 cut(s) 975
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 1 cut(s) 143
AvrII CCTAGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 208
BamHI GGATCC 1 cut(s) 389
BanII GRGCYC 2 cut(s) 36, 99
BauI CACGAG 2 cut(s) 56, 924
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 36, 99
BbvI GCAGC 2 cut(s) 712, 1024
BccI CCATC 2 cut(s) 442, 1163
BceAI ACGGC 1 cut(s) 186
BcgI CGANNNNNNTGC 2 cut(s) 1173, 1207
BciT130I CCWGG 2 cut(s) 156, 1103
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 4 cut(s) 140, 455, 636, 1151
BisI GCNGC 3 cut(s) 9, 701, 1013
BlnI CCTAGG 1 cut(s) 139
BlsI GCNGC 3 cut(s) 10, 702, 1014
Bme1390I CCNGG 2 cut(s) 156, 1103
Bme18I GGWCC 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 143, 152
BmiI GGNNCC 2 cut(s) 145, 391
BmrFI CCNGG 2 cut(s) 156, 1103
BpiI GAAGAC 1 cut(s) 152
BplI GAGNNNNNCTC 6 cut(s) 515, 547, 918, 950, 1061, 1093
Bpu14I TTCGAA 1 cut(s) 975
BpuEI CTTGAG 2 cut(s) 251, 527
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 139, 154, 1053, 1065
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 503, 533
Bse118I RCCGGY 2 cut(s) 173, 1015
Bse3DI GCAATG 4 cut(s) 339, 603, 710, 1154
BseBI CCWGG 2 cut(s) 156, 1103
BseDI CCNNGG 4 cut(s) 139, 154, 1053, 1065
BseGI GGATG 2 cut(s) 766, 1179
BseMI GCAATG 4 cut(s) 339, 603, 710, 1154
BseMII CTCAG 3 cut(s) 300, 870, 1092
BseRI GAGGAG 2 cut(s) 200, 524
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 2 cut(s) 712, 1024
Bsh1236I CGCG 2 cut(s) 1195, 1197
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 4 cut(s) 153, 459, 872, 964
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 2 cut(s) 36, 99
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 2 cut(s) 174, 1016
BslFI GGGAC 2 cut(s) 129, 164
BsmAI GTCTC 1 cut(s) 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 129, 164
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 4 cut(s) 153, 459, 872, 964
BsoBI CYCGRG 1 cut(s) 471
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 3 cut(s) 36, 99, 208
Bsp143I GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 7 cut(s) 9, 37, 107, 189, 199, 566, 1159
BspANI GGCC 4 cut(s) 153, 459, 872, 964
BspCNI CTCAG 3 cut(s) 301, 871, 1091
BspFNI CGCG 2 cut(s) 1195, 1197
BspLI GGNNCC 2 cut(s) 145, 391
BspPI GGATC 6 cut(s) 279, 384, 397, 798, 833, 1231
BspQI GCTCTTC 1 cut(s) 87
BspT104I TTCGAA 1 cut(s) 975
BsrDI GCAATG 4 cut(s) 339, 603, 710, 1154
BsrFI RCCGGY 2 cut(s) 173, 1015
BssAI RCCGGY 2 cut(s) 173, 1015
BssECI CCNNGG 4 cut(s) 139, 154, 1053, 1065
BssMI GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 2 cut(s) 56, 924
BssT1I CCWWGG 3 cut(s) 139, 1053, 1065
Bst2BI CACGAG 2 cut(s) 56, 924
Bst2UI CCWGG 2 cut(s) 156, 1103
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 2 cut(s) 87, 602
BstACI GRCGYC 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 5 cut(s) 223, 227, 231, 962, 1017
BstDEI CTNAG 3 cut(s) 309, 879, 1078
BstDSI CCRYGG 1 cut(s) 1053
BstF5I GGATG 2 cut(s) 766, 1179
BstFNI CGCG 2 cut(s) 1195, 1197
BstHHI GCGC 1 cut(s) 1197
BstKTI GATC 6 cut(s) 274, 392, 484, 793, 841, 1226
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 2 cut(s) 706, 1114
BstNI CCWGG 2 cut(s) 156, 1103
BstSCI CCNGG 2 cut(s) 154, 1101
BstSLI GKGCMC 1 cut(s) 208
BstUI CGCG 2 cut(s) 1195, 1197
BstV1I GCAGC 2 cut(s) 712, 1024
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 2 cut(s) 389, 1223
BstYI RGATCY 2 cut(s) 389, 1223
BsuRI GGCC 4 cut(s) 153, 459, 872, 964
BtgI CCRYGG 1 cut(s) 1053
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 2 cut(s) 766, 1179
Cac8I GCNNGC 5 cut(s) 223, 227, 231, 962, 1017
CfoI GCGC 1 cut(s) 1197
Cfr10I RCCGGY 2 cut(s) 173, 1015
Cfr13I GGNCC 2 cut(s) 143, 152
CseI GACGC 1 cut(s) 166
Csp6I GTAC 2 cut(s) 53, 1142
CviAII CATG 6 cut(s) 353, 386, 445, 604, 862, 1054
CviQI GTAC 2 cut(s) 53, 1142
DdeI CTNAG 3 cut(s) 309, 879, 1078
DpnI GATC 6 cut(s) 273, 391, 483, 792, 840, 1225
DpnII GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
Eam1104I CTCTTC 2 cut(s) 87, 602
EarI CTCTTC 2 cut(s) 87, 602
EciI GGCGGA 3 cut(s) 122, 178, 188
Ecl136II GAGCTC 2 cut(s) 34, 97
Eco130I CCWWGG 3 cut(s) 139, 1053, 1065
Eco147I AGGCCT 3 cut(s) 459, 872, 964
Eco24I GRGCYC 2 cut(s) 36, 99
Eco32I GATATC 2 cut(s) 921, 1074
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 2 cut(s) 34, 97
Eco57I CTGAAG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 2 cut(s) 34, 97
EcoO109I RGGNCCY 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 2 cut(s) 154, 1101
EcoRV GATATC 2 cut(s) 921, 1074
EcoT14I CCWWGG 3 cut(s) 139, 1053, 1065
EcoT38I GRGCYC 2 cut(s) 36, 99
ErhI CCWWGG 3 cut(s) 139, 1053, 1065
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 6 cut(s) 356, 389, 448, 607, 865, 1057
FaqI GGGAC 2 cut(s) 129, 164
FatI CATG 6 cut(s) 352, 385, 444, 603, 861, 1053
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 9, 701, 1013
FokI GGATG 2 cut(s) 753, 1186
FriOI GRGCYC 2 cut(s) 36, 99
Fsp4HI GCNGC 3 cut(s) 9, 701, 1013
FspBI CTAG 4 cut(s) 140, 455, 636, 1151
GlaI GCGC 1 cut(s) 1196
GluI GCNGC 3 cut(s) 9, 701, 1013
HaeIII GGCC 4 cut(s) 153, 459, 872, 964
HapII CCGG 2 cut(s) 174, 1016
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 1197
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 6 cut(s) 356, 389, 448, 607, 865, 1057
Hin6I GCGC 1 cut(s) 1195
HinP1I GCGC 1 cut(s) 1195
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 5 cut(s) 74, 110, 409, 524, 905
HpaII CCGG 2 cut(s) 174, 1016
HphI GGTGA 4 cut(s) 555, 665, 721, 877
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 5 cut(s) 46, 382, 416, 688, 1081
Hpy188III TCNNGA 4 cut(s) 736, 794, 878, 924
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 2 cut(s) 88, 118
HpyAV CCTTC 7 cut(s) 245, 637, 779, 862, 956, 975, 1107
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4V TGCA 6 cut(s) 337, 444, 960, 1012, 1184, 1234
HpyF10VI GCNNNNNNNGC 2 cut(s) 706, 1114
HpyF3I CTNAG 3 cut(s) 309, 879, 1078
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 6 cut(s) 356, 389, 448, 607, 865, 1057
HspAI GCGC 1 cut(s) 1195
KroI GCCGGC 1 cut(s) 1015
KroNI GCCGGC 1 cut(s) 1017
Kzo9I GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 5 cut(s) 39, 451, 748, 939, 1105
Lsp1109I GCAGC 2 cut(s) 712, 1024
MaeI CTAG 4 cut(s) 140, 455, 636, 1151
MaeIII GTNAC 2 cut(s) 653, 1039
MalI GATC 6 cut(s) 273, 391, 483, 792, 840, 1225
MboI GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
MboII GAAGA 6 cut(s) 104, 152, 602, 619, 794, 1136
MflI RGATCY 2 cut(s) 389, 1223
MhlI GDGCHC 3 cut(s) 36, 99, 208
MluCI AATT 5 cut(s) 376, 631, 643, 979, 1137
MlyI GAGTC 3 cut(s) 119, 533, 914
MmeI TCCRAC 2 cut(s) 69, 501
MroNI GCCGGC 1 cut(s) 1015
MseI TTAA 1 cut(s) 462
MspI CCGG 2 cut(s) 174, 1016
MspR9I CCNGG 2 cut(s) 156, 1103
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 2 cut(s) 156, 1103
MvnI CGCG 2 cut(s) 1195, 1197
MwoI GCNNNNNNNGC 2 cut(s) 706, 1114
NaeI GCCGGC 1 cut(s) 1017
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
NgoMIV GCCGGC 1 cut(s) 1015
NlaIII CATG 6 cut(s) 356, 389, 448, 607, 865, 1057
NlaIV GGNNCC 2 cut(s) 145, 391
NmuCI GTSAC 1 cut(s) 653
NspV TTCGAA 1 cut(s) 975
PaeR7I CTCGAG 1 cut(s) 471
PceI AGGCCT 3 cut(s) 459, 872, 964
PciSI GCTCTTC 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 1199
PctI GAATGC 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 1017
PfeI GAWTC 2 cut(s) 74, 409
PkrI GCNGC 3 cut(s) 10, 702, 1014
Ple19I CGATCG 1 cut(s) 484
PleI GAGTC 3 cut(s) 118, 532, 913
PpsI GAGTC 3 cut(s) 118, 532, 913
PpuMI RGGWCCY 1 cut(s) 143
Psp124BI GAGCTC 2 cut(s) 36, 99
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 154, 1101
PspGI CCWGG 2 cut(s) 154, 1101
PspN4I GGNNCC 2 cut(s) 145, 391
PspPI GGNCC 2 cut(s) 143, 152
PspPPI RGGWCCY 1 cut(s) 143
PspXI VCTCGAGB 1 cut(s) 471
PsuI RGATCY 2 cut(s) 389, 1223
PvuI CGATCG 1 cut(s) 484
RsaI GTAC 2 cut(s) 54, 1143
RsaNI GTAC 2 cut(s) 53, 1142
SacI GAGCTC 2 cut(s) 36, 99
SalI GTCGAC 1 cut(s) 112
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 3 cut(s) 9, 701, 1013
Sau3AI GATC 6 cut(s) 271, 389, 481, 790, 838, 1223
Sau96I GGNCC 2 cut(s) 143, 152
SchI GAGTC 3 cut(s) 119, 533, 914
ScrFI CCNGG 2 cut(s) 156, 1103
SduI GDGCHC 3 cut(s) 36, 99, 208
Sfr274I CTCGAG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 975
SinI GGWCC 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 471
SmlI CTYRAG 3 cut(s) 266, 471, 506
SmoI CTYRAG 3 cut(s) 266, 471, 506
Sse9I AATT 5 cut(s) 376, 631, 643, 979, 1137
SseBI AGGCCT 3 cut(s) 459, 872, 964
SsiI CCGC 7 cut(s) 9, 37, 107, 189, 199, 566, 1159
SspI AATATT 2 cut(s) 347, 834
SspMI CTAG 4 cut(s) 140, 455, 636, 1151
SstI GAGCTC 2 cut(s) 36, 99
StuI AGGCCT 3 cut(s) 459, 872, 964
StyD4I CCNGG 2 cut(s) 154, 1101
StyI CCWWGG 3 cut(s) 139, 1053, 1065
TaaI ACNGT 1 cut(s) 733
TaqI TCGA 5 cut(s) 99, 113, 472, 617, 975
TasI AATT 5 cut(s) 376, 631, 643, 979, 1137
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 74, 409
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 700, 1012
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 4 cut(s) 87, 378, 620, 850
TspGWI ACGGA 4 cut(s) 382, 568, 926, 1027
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 2 cut(s) 376, 979
XhoI CTCGAG 1 cut(s) 471
XmaJI CCTAGG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 4 cut(s) 140, 455, 636, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.