pycom03g20740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016094
Physical Location & Seq
Reverse (-)
21173345 .. 21175222
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20740.2

Sequence Viewer

Length: 1248 bp
ATGGAAGCCGCCGAAGTCCTACAACTCTACGAGCTCCCCTACTCCGATCTCGTACTCGTGTCGTCAAACGATGAATCATTGTCGTCGGCAGGAGAGCTCGAAAGGGCGGAGTCGACGAGCAAAGCCATAATGGAAGCCCTAGGTCCCGCTGGCCCAGGCCTTCTCGCCATTACCGGCGTCCCCAAATCCACCTCTCTCCGCCGTGCCCTCCTCCCTCTCGCTCGCAAGCTCGCCCTTCTCAACCCTAATCATCGCAAAGGCATTCTCAAGGATCACAACTTGGGAAGTGATGTGCCTTTGAAAAACCCTGAGAGAAATGTGTCGTCTTTCGCAATGCAAATCAAATATTCACATGATTTTGATGAACCCGAATTGAATTCAGAACATGGATCCGTGAACGAGTTTGAGAATCTCGGAAATGGATTTAGGGAGTTGGGTTTTTGCATGATGGAGCTAGGCCTTAAACTTGCTCGAGTATGCGATCGTGCCATTGGTGGAAATGAGCTTGAGGAGAGCTTGTTGGAGTCCTGCTCTGCCAAAGCTCGTCTCATTCACTATCATTCACCGCTTGATAGTTCCATTCTTGTTGAAGAAGCAATGAGCACGAAGAGAACATCGAAAAGACCCTTCAATTCTAGCAGGAATTGGATTGGTGACAAATGCAAACAAGTTTCGGGGATTTGCTCTGATAACCTGTGGCAGCAATGGCATTATGACTACGGGATTTTCACCGTCTTGACAGCTCCGATGTTTCTCTTGTCAAATCATCCACAGGCGAAAGAAGAAAGGGACCAAGAGTATGCTTACCCTAATGGAAATACTTATTTACAAATATTTGATCCCAGAAAGGATAATGTGTTCATGGTGAAGGCCTCTCCTGAGAGTTTTATAGTTCAGGTTGGCGAGTCGGCTAATATCATATCACGAGGAAAGCTCCATGCCACCCTTCACTCTGTTTGCAGGCCTTCCAAGTTCGAAAATTTGAGCAGAGAAACTTTTGTTGTGTTCTTGCAGCCGGCGTGGGACAAAACTTTCTCTGTAACAGATTATTCCATGGACAAATCCTTGGAGATATCATCAGAGGTCAAACAGGTTGATGATCCGGAACAAAGTAGGCTAACTGAAGAATTACAGAAAATTGTACCACCTCTGGCATTGCGGTTGAAAGATGGGATGACATTTGCAGATTTCTCGCGCGAAACGACAAAGCAATACTATGGTGGATCTGGTTTGCAGTCAAATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy

Protein Analysis

416

Amino Acids

46.51

Weight (kDa)

5.59

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63290 AT3G63290
fragaria_vesca FvH4_2g23081
malus_domestica MD03G1259400.v1.1
prunus_persica Prupe.8G235900_v2.0.a1
pyrus_communis pycom03g20740
rosa_chinensis RchiOBHm_Chr6g0290231
rosa_laevigata RLG00000012232
rosa_roxburghii Rroxscaffold_7G00177220
rosa_rugosa Rorug06G0211100
rosa_samantha Rh6AG321400 Rh6BG328500 Rh6CG334500 Rh6DG321500
rosa_wichuraiana Rw6G027760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 113
AccII CGCG 2 cut(s) 1195, 1197
AccIII TCCGGA 1 cut(s) 1102
AciI CCGC 6 cut(s) 9, 107, 147, 199, 566, 1159
AclWI GGATC 6 cut(s) 279, 384, 397, 833, 1094, 1231
AcsI RAATTY 2 cut(s) 376, 979
AcuI CTGAAG 1 cut(s) 1143
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 2 cut(s) 54, 1143
AgsI TTSAA 5 cut(s) 301, 376, 590, 631, 1165
AjnI CCWGG 1 cut(s) 154
AloI GAACNNNNNNTCC 2 cut(s) 842, 874
AluBI AGCT 9 cut(s) 34, 97, 229, 454, 505, 516, 542, 743, 934
AluI AGCT 9 cut(s) 34, 97, 229, 454, 505, 516, 542, 743, 934
Alw21I GWGCWC 3 cut(s) 36, 99, 605
Alw26I GTCTC 1 cut(s) 551
AlwI GGATC 6 cut(s) 279, 384, 397, 833, 1094, 1231
Ama87I CYCGRG 1 cut(s) 471
Aor13HI TCCGGA 1 cut(s) 1102
AoxI GGCC 5 cut(s) 151, 157, 457, 870, 962
ApeKI GCWGC 2 cut(s) 700, 1012
ApoI RAATTY 2 cut(s) 376, 979
AspA2I CCTAGG 1 cut(s) 139
AspLEI GCGC 1 cut(s) 1197
AspS9I GGNCC 3 cut(s) 143, 152, 790
AsuHPI GGTGA 4 cut(s) 555, 665, 721, 877
AsuII TTCGAA 1 cut(s) 975
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 2 cut(s) 143, 790
AvrII CCTAGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 208
BamHI GGATCC 1 cut(s) 389
BanII GRGCYC 2 cut(s) 36, 99
BauI CACGAG 2 cut(s) 56, 924
Bbv12I GWGCWC 3 cut(s) 36, 99, 605
BbvI GCAGC 2 cut(s) 712, 1024
BccI CCATC 2 cut(s) 442, 1163
BceAI ACGGC 1 cut(s) 186
BcgI CGANNNNNNTGC 2 cut(s) 1173, 1207
BciT130I CCWGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 3 cut(s) 140, 455, 636
BisI GCNGC 3 cut(s) 9, 701, 1013
BlnI CCTAGG 1 cut(s) 139
BlsI GCNGC 3 cut(s) 10, 702, 1014
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 2 cut(s) 143, 790
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 3 cut(s) 143, 152, 790
BmiI GGNNCC 3 cut(s) 145, 391, 791
BmrFI CCNGG 1 cut(s) 156
BplI GAGNNNNNCTC 4 cut(s) 515, 547, 918, 950
Bpu14I TTCGAA 1 cut(s) 975
BpuEI CTTGAG 2 cut(s) 251, 527
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 139, 154, 1053, 1065
BsaWI WCCGGW 1 cut(s) 1102
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bse118I RCCGGY 2 cut(s) 173, 1015
Bse3DI GCAATG 4 cut(s) 339, 603, 710, 1154
BseAI TCCGGA 1 cut(s) 1102
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 4 cut(s) 139, 154, 1053, 1065
BseGI GGATG 2 cut(s) 766, 1179
BseMI GCAATG 4 cut(s) 339, 603, 710, 1154
BseMII CTCAG 2 cut(s) 300, 870
BseRI GAGGAG 2 cut(s) 200, 524
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 2 cut(s) 712, 1024
Bsh1236I CGCG 2 cut(s) 1195, 1197
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 5 cut(s) 153, 159, 459, 872, 964
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 3 cut(s) 36, 99, 605
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 3 cut(s) 174, 1016, 1103
BslFI GGGAC 4 cut(s) 129, 164, 803, 1037
BsmAI GTCTC 1 cut(s) 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 4 cut(s) 129, 164, 803, 1037
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 5 cut(s) 153, 159, 459, 872, 964
BsoBI CYCGRG 1 cut(s) 471
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 4 cut(s) 36, 99, 208, 605
Bsp13I TCCGGA 1 cut(s) 1102
Bsp143I GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 6 cut(s) 9, 107, 147, 199, 566, 1159
BspANI GGCC 5 cut(s) 153, 159, 459, 872, 964
BspCNI CTCAG 2 cut(s) 301, 871
BspEI TCCGGA 1 cut(s) 1102
BspFNI CGCG 2 cut(s) 1195, 1197
BspLI GGNNCC 3 cut(s) 145, 391, 791
BspPI GGATC 6 cut(s) 279, 384, 397, 833, 1094, 1231
BspT104I TTCGAA 1 cut(s) 975
BsrDI GCAATG 4 cut(s) 339, 603, 710, 1154
BsrFI RCCGGY 2 cut(s) 173, 1015
BssAI RCCGGY 2 cut(s) 173, 1015
BssECI CCNNGG 4 cut(s) 139, 154, 1053, 1065
BssMI GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 2 cut(s) 56, 924
BssT1I CCWWGG 3 cut(s) 139, 1053, 1065
Bst2BI CACGAG 2 cut(s) 56, 924
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 1 cut(s) 602
BstACI GRCGYC 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 6 cut(s) 151, 223, 227, 231, 962, 1017
BstDEI CTNAG 2 cut(s) 309, 879
BstDSI CCRYGG 1 cut(s) 1053
BstF5I GGATG 2 cut(s) 766, 1179
BstFNI CGCG 2 cut(s) 1195, 1197
BstHHI GCGC 1 cut(s) 1197
BstKTI GATC 7 cut(s) 49, 274, 392, 484, 841, 1102, 1226
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 1 cut(s) 706
BstNI CCWGG 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 208
BstUI CGCG 2 cut(s) 1195, 1197
BstV1I GCAGC 2 cut(s) 712, 1024
BstX2I RGATCY 2 cut(s) 389, 1223
BstYI RGATCY 2 cut(s) 389, 1223
BsuRI GGCC 5 cut(s) 153, 159, 459, 872, 964
BtgI CCRYGG 1 cut(s) 1053
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 2 cut(s) 766, 1179
Cac8I GCNNGC 6 cut(s) 151, 223, 227, 231, 962, 1017
CfoI GCGC 1 cut(s) 1197
Cfr10I RCCGGY 2 cut(s) 173, 1015
Cfr13I GGNCC 3 cut(s) 143, 152, 790
CseI GACGC 1 cut(s) 166
Csp6I GTAC 2 cut(s) 53, 1142
CviAII CATG 6 cut(s) 353, 386, 445, 862, 938, 1054
CviQI GTAC 2 cut(s) 53, 1142
DdeI CTNAG 2 cut(s) 309, 879
DpnI GATC 7 cut(s) 48, 273, 391, 483, 840, 1101, 1225
DpnII GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
EciI GGCGGA 2 cut(s) 122, 188
Ecl136II GAGCTC 2 cut(s) 34, 97
Eco130I CCWWGG 3 cut(s) 139, 1053, 1065
Eco147I AGGCCT 4 cut(s) 159, 459, 872, 964
Eco24I GRGCYC 2 cut(s) 36, 99
Eco32I GATATC 1 cut(s) 1074
Eco47I GGWCC 2 cut(s) 143, 790
Eco53kI GAGCTC 2 cut(s) 34, 97
Eco57I CTGAAG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 2 cut(s) 34, 97
EcoO109I RGGNCCY 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 154
EcoRV GATATC 1 cut(s) 1074
EcoT14I CCWWGG 3 cut(s) 139, 1053, 1065
EcoT38I GRGCYC 2 cut(s) 36, 99
ErhI CCWWGG 3 cut(s) 139, 1053, 1065
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 6 cut(s) 356, 389, 448, 865, 941, 1057
FaqI GGGAC 4 cut(s) 129, 164, 803, 1037
FatI CATG 6 cut(s) 352, 385, 444, 861, 937, 1053
FauI CCCGC 1 cut(s) 154
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 9, 701, 1013
FokI GGATG 2 cut(s) 753, 1186
FriOI GRGCYC 2 cut(s) 36, 99
Fsp4HI GCNGC 3 cut(s) 9, 701, 1013
FspBI CTAG 3 cut(s) 140, 455, 636
GlaI GCGC 1 cut(s) 1196
GluI GCNGC 3 cut(s) 9, 701, 1013
HaeIII GGCC 5 cut(s) 153, 159, 459, 872, 964
HapII CCGG 3 cut(s) 174, 1016, 1103
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 1197
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 6 cut(s) 356, 389, 448, 865, 941, 1057
Hin6I GCGC 1 cut(s) 1195
HinP1I GCGC 1 cut(s) 1195
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 5 cut(s) 74, 110, 409, 524, 905
HpaII CCGG 3 cut(s) 174, 1016, 1103
HphI GGTGA 4 cut(s) 555, 665, 721, 877
Hpy166II GTNNAC 2 cut(s) 114, 397
Hpy188I TCNGA 6 cut(s) 46, 382, 416, 688, 747, 1081
Hpy188III TCNNGA 4 cut(s) 736, 878, 924, 1103
Hpy8I GTNNAC 2 cut(s) 114, 397
Hpy99I CGWCG 2 cut(s) 88, 118
HpyAV CCTTC 6 cut(s) 170, 245, 637, 862, 956, 975
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4V TGCA 7 cut(s) 337, 444, 663, 960, 1012, 1184, 1234
HpyF10VI GCNNNNNNNGC 1 cut(s) 706
HpyF3I CTNAG 2 cut(s) 309, 879
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 6 cut(s) 356, 389, 448, 865, 941, 1057
HspAI GCGC 1 cut(s) 1195
Kpn2I TCCGGA 1 cut(s) 1102
KroI GCCGGC 1 cut(s) 1015
KroNI GCCGGC 1 cut(s) 1017
Kzo9I GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
LmnI GCTCC 4 cut(s) 39, 451, 748, 939
Lsp1109I GCAGC 2 cut(s) 712, 1024
MaeI CTAG 3 cut(s) 140, 455, 636
MaeIII GTNAC 2 cut(s) 653, 1039
MalI GATC 7 cut(s) 48, 273, 391, 483, 840, 1101, 1225
MboI GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
MboII GAAGA 4 cut(s) 602, 619, 794, 1136
MflI RGATCY 2 cut(s) 389, 1223
MhlI GDGCHC 4 cut(s) 36, 99, 208, 605
MluCI AATT 7 cut(s) 371, 376, 631, 643, 979, 1127, 1137
MlyI GAGTC 3 cut(s) 119, 533, 914
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 9 cut(s) 202, 218, 221, 225, 502, 883, 920, 1075, 1158
MroI TCCGGA 1 cut(s) 1102
MroNI GCCGGC 1 cut(s) 1015
MseI TTAA 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 3 cut(s) 174, 1016, 1103
MspR9I CCNGG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 1 cut(s) 156
MvnI CGCG 2 cut(s) 1195, 1197
MwoI GCNNNNNNNGC 1 cut(s) 706
NaeI GCCGGC 1 cut(s) 1017
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
NgoMIV GCCGGC 1 cut(s) 1015
NlaIII CATG 6 cut(s) 356, 389, 448, 865, 941, 1057
NlaIV GGNNCC 3 cut(s) 145, 391, 791
NmuCI GTSAC 1 cut(s) 653
NspV TTCGAA 1 cut(s) 975
PaeR7I CTCGAG 1 cut(s) 471
PceI AGGCCT 4 cut(s) 159, 459, 872, 964
PcsI WCGNNNNNNNCGW 1 cut(s) 1199
PctI GAATGC 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 1017
PfeI GAWTC 2 cut(s) 74, 409
PkrI GCNGC 3 cut(s) 10, 702, 1014
Ple19I CGATCG 1 cut(s) 484
PleI GAGTC 3 cut(s) 118, 532, 913
PpsI GAGTC 3 cut(s) 118, 532, 913
PpuMI RGGWCCY 1 cut(s) 143
Psp124BI GAGCTC 2 cut(s) 36, 99
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 3 cut(s) 145, 391, 791
PspPI GGNCC 3 cut(s) 143, 152, 790
PspPPI RGGWCCY 1 cut(s) 143
PspXI VCTCGAGB 1 cut(s) 471
PsuI RGATCY 2 cut(s) 389, 1223
PvuI CGATCG 1 cut(s) 484
RsaI GTAC 2 cut(s) 54, 1143
RsaNI GTAC 2 cut(s) 53, 1142
SacI GAGCTC 2 cut(s) 36, 99
SalI GTCGAC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 3 cut(s) 9, 701, 1013
Sau3AI GATC 7 cut(s) 46, 271, 389, 481, 838, 1099, 1223
Sau96I GGNCC 3 cut(s) 143, 152, 790
SchI GAGTC 3 cut(s) 119, 533, 914
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 4 cut(s) 36, 99, 208, 605
Sfr274I CTCGAG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 975
SinI GGWCC 2 cut(s) 143, 790
SlaI CTCGAG 1 cut(s) 471
SmlI CTYRAG 3 cut(s) 266, 471, 506
SmoI CTYRAG 3 cut(s) 266, 471, 506
Sse9I AATT 7 cut(s) 371, 376, 631, 643, 979, 1127, 1137
SseBI AGGCCT 4 cut(s) 159, 459, 872, 964
SsiI CCGC 6 cut(s) 9, 107, 147, 199, 566, 1159
SspI AATATT 2 cut(s) 347, 834
SspMI CTAG 3 cut(s) 140, 455, 636
SstI GAGCTC 2 cut(s) 36, 99
StuI AGGCCT 4 cut(s) 159, 459, 872, 964
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 3 cut(s) 139, 1053, 1065
TaaI ACNGT 1 cut(s) 733
TaqI TCGA 5 cut(s) 99, 113, 472, 617, 975
TasI AATT 7 cut(s) 371, 376, 631, 643, 979, 1127, 1137
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 74, 409
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 700, 1012
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 3 cut(s) 87, 378, 850
TspGWI ACGGA 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 143, 790
XapI RAATTY 2 cut(s) 376, 979
XhoI CTCGAG 1 cut(s) 471
XmaJI CCTAGG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 3 cut(s) 140, 455, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.